MD04G1135600.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012635
Physical Location & Seq
Reverse (-)
22245201 .. 22250758
5558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1135600.v1.1.491

Sequence Viewer

Length: 2298 bp
ATGGGGACTACTGCTCTGAGGCAGTTGCTGAATAACCTCTGCAGCAATTCGCCTTGGAATTATGCGGTGTTTTGGAAGCTCAAGCACCAGAGTGAATTGATTTTGTTGTGGGAGGATGGGTACTGTCATCACCCAAAGCCAAGAGAAGCTGTGGAACATGCATCGGATGATATTTATTTCAGCAACGCAAATCAGATGCTTTTTAAAGGTTTTGCAACAAGTATACATGATGGTGGATCTACAGGATATCCAATTGGACTAGCAGTGGCTGACATGTCATATCTTCAGCACACATTTGGAAAAGGGGTTGTTGGTGAGGTGGCATGTACAGGAAACTATAGCTGGGTATTCCTTGACAATCTGTGCACCAGAGAGTCTGGCTCTAAGTTAGTTCCTGAGTGTCCAGATGAATGGATTCTTCAGTTTTCATCGGGTATCAAGACAATTTTACTTGTACCTGTGCTTCCATATGGAGTTTTGCAACTTGGCTCATTGGAGACGGTTGCTGAAGATCCAACAGTGGTTGCTTTTGTGAAAGATAGATTCAATGACATTCACAATGGTGTGGGGAAAACTGTACCTTACCGTCAAGCTCAATCGTCATGGTCACAATCATCAAATTTAATGGAGACCACATTTGAGCCATCAGGTGTTACCATTAACCCACTGAAAGTTGAAAAGTCAGAAGATGTTGATAACATCAGATATAATAACCTATTGTCGACATTGGGGCACTTTTTGCCAACTGCTGAAGATTCACTTCTAGAGACCGGAACAGATCAGCCAGAAGTTCTAAAGAGTAGAGACGAAAGTGTGACTGGTGTTCCACCAATTTATCACAGTGGAGAATCAAGTCCACTCAGTCAGTCGATAGATACCAGCTTGCTGGGTATGATGGAAAGTCAGATGTTTGGGTTGTCTTGTCTGGAGGAAGAACTCCTAGCCTATTCTCAATTTGGTGGTTACAATTTGGAAGTGTTTGGAGAATCCATGAGCGATTTCACTTCTTACCCTGTTGGAGGTGTAGCTGAACTGTTCGAAGACGTTAATGCTAAAGATACAAATTACAACATGGTGAATAATTTCTTTAGCTTTCCTGAAAATTGTGAACTACACAAAGCACTTGGAACACTTCCGCAGAGGCAGACTGACGAAAATTTATGGGATCCATCCATTGGCATTGATGATACGTGTAGCAGCTCTAGTTTGCAGAAAGATCTTGCTAGCCGTATTGAGCCATCATGGTTTTCCAAAGGAAGTGATGCTGAGAACCTCGTGAAAGCGTTTGCAGCCAAAGATGAAACCTCATCCAGCCAATCAGACAATATTAAATCATCTATCACCTCATCAAGTCAATTTCCTGCTTCTTTTAAACAATTGAAATTTGAAGCGTGTGCCCCAATAGGAAGTGGTTCGGTGACATGGAACCAGTCATCTGCTTTACTTGCCAGGGGTGCTACTCCAGCTTCTTTCACAGACATGATGAGCACTTTGGTTGACGAAGAGCAGCAGGGCAAAGGGTACAAATCTGTGAAGCCTAAAAGGGAACAAAAGTTATCCGGTGGCACTGCAAGGAAGACCAAGATTGGCAATAGCCCGAAGCTAAGACCAAGAGATAGACAACTGATTCAAGATCGTGTCAAGGAGCTGCGGGAACTTGTCCCAAATGGTGCCAAGTGCAGCATTGATGGCCTTTTAGACCGAACCATAAAACACATGCAGTTTTTAAGAAACATGACTGATCAAGCTGAAAAACTGAAGTGCTACACACAGCAAGAGGCACCTCGTTCCAATAACATGAGCGAAGCCACAAGTGGCTGTGAAAATGGGACAAGCAGGGCTTTTGAAGTTGGAAGCGAACACCAGATTTGCCCCATTGTGGTAGAAGATCTTGAACACTCGGGACACATTCTCATAGAGATGCTATGTGATGAACATGGACTTTTCTTAGACATAGCCCAAGCTATCCGACGGTTGGAACTGACTATCTTGAAGGGTGTCATGGAAACTCGCTCAAGCAACATGTGGGCACACTTTGTTGTTGAGAATGGATGTGTTTTGGCCGCTGCTGCATCTTCTGCAACGCAGAAGAAGTTCAATATCAAGCAGGATATGATGCGCCTCCATTTGCATCCAAGAAAAGCGGGTAAGCCGTCAAATATTGTGCTTCGAAACCACATGACGACGACGAGACCTAGGAGACATCACCATCAATCCTCATCAGAAGACTGTAAATTATCAAATGGGTTGCAGAAGATTTCTATGCAATTTGATGAAAACCAGCATCTTAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bHLH-MYC_N

Protein Analysis

766

Amino Acids

84.86

Weight (kDa)

5.59

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 6 - 182 3.5e-32 bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_LHW PF23176 536 - 587 3e-28 Transcription factor LHW bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13090 FvH4_6g13090 FvH4_6g13090 FvH4_6g13090 FvH4_6g13090
malus_domestica MD04G1135600.v1.1 MD12G1149300.v1.1
prunus_persica Prupe.6G260900_v2.0.a1
pyrus_communis pycom04g12270 pycom12g14310
rosa_chinensis RchiOBHm_Chr3g0465361
rosa_laevigata RLG00000024643
rosa_multiflora Rmu_sc0011781.1_g000003
rosa_roxburghii Rroxscaffold_6G00415100
rosa_rugosa Rorug03G0076300
rosa_samantha Rh3BG141300 Rh3CG142400 Rh3DG142400
rosa_wichuraiana Rw3G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1670, 1780
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccI GTMKAC 2 cut(s) 223, 722
AciI CCGC 5 cut(s) 65, 1136, 1651, 2064, 2144
AclWI GGATC 4 cut(s) 244, 506, 1160, 1173
AcoI YGGCCR 1 cut(s) 2061
AcsI RAATTY 3 cut(s) 619, 1156, 1382
AcuI CTGAAG 5 cut(s) 269, 404, 528, 771, 1778
AfaI GTAC 5 cut(s) 122, 328, 456, 579, 1523
AfiI CCNNNNNNNGG 4 cut(s) 1019, 1175, 1879, 1975
AflIII ACRYGT 3 cut(s) 273, 1190, 2022
AgsI TTSAA 9 cut(s) 547, 677, 1381, 1388, 1631, 1847, 1895, 1993, 2098
AjnI CCWGG 1 cut(s) 1448
AleI CACNNNNGTG 2 cut(s) 90, 561
Alw21I GWGCWC 2 cut(s) 368, 1490
Alw26I GTCTC 6 cut(s) 491, 623, 761, 798, 2185, 2194
Alw44I GTGCAC 1 cut(s) 364
AlwI GGATC 4 cut(s) 244, 506, 1160, 1173
AlwNI CAGNNNCTG 2 cut(s) 28, 269
Ama87I CYCGRG 1 cut(s) 1900
AoxI GGCC 2 cut(s) 1690, 2061
ApaLI GTGCAC 1 cut(s) 364
ApeKI GCWGC 8 cut(s) 42, 1197, 1289, 1507, 1648, 1680, 2066, 2069
ApoI RAATTY 3 cut(s) 619, 1156, 1382
Asp700I GAANNNNTTC 4 cut(s) 414, 1082, 1411, 2093
AspA2I CCTAGG 1 cut(s) 2195
AspLEI GCGC 1 cut(s) 2121
AsuHPI GGTGA 6 cut(s) 122, 326, 1087, 1333, 1429, 2198
AsuII TTCGAA 2 cut(s) 1038, 2170
AsuNHI GCTAGC 1 cut(s) 1223
AvaI CYCGRG 1 cut(s) 1900
AvrII CCTAGG 1 cut(s) 2195
BaeGI GKGCMC 4 cut(s) 368, 735, 1399, 2032
BamHI GGATCC 1 cut(s) 1165
BanI GGYRCC 2 cut(s) 1670, 1780
BarI GAAGNNNNNNTAC 2 cut(s) 447, 479
BauI CACGAG 1 cut(s) 1274
BbsI GAAGAC 3 cut(s) 1047, 1583, 2232
Bbv12I GWGCWC 2 cut(s) 368, 1490
BbvI GCAGC 8 cut(s) 54, 1209, 1301, 1519, 1635, 1692, 2053, 2056
BccI CCATC 8 cut(s) 110, 224, 652, 889, 1177, 1246, 1682, 2217
BceAI ACGGC 2 cut(s) 1212, 2137
BciT130I CCWGG 1 cut(s) 1450
BclI TGATCA 1 cut(s) 1741
BcoDI GTCTC 6 cut(s) 491, 623, 761, 798, 2185, 2194
BfaI CTAG 7 cut(s) 260, 764, 941, 1203, 1224, 2196, 2296
BfmI CTRYAG 3 cut(s) 40, 240, 337
BglII AGATCT 2 cut(s) 1216, 1888
BisI GCNGC 9 cut(s) 43, 1198, 1290, 1508, 1649, 1681, 2064, 2067, 2070
BlnI CCTAGG 1 cut(s) 2195
BlsI GCNGC 9 cut(s) 44, 1199, 1291, 1509, 1650, 1682, 2065, 2068, 2071
Bme1390I CCNGG 1 cut(s) 1450
BmeT110I CYCGRG 1 cut(s) 1900
BmiI GGNNCC 4 cut(s) 1167, 1427, 1672, 1782
BmrFI CCNGG 1 cut(s) 1450
BmsI GCATC 8 cut(s) 170, 186, 1252, 1911, 2081, 2106, 2140, 2293
BmtI GCTAGC 1 cut(s) 1227
BpiI GAAGAC 3 cut(s) 1047, 1583, 2232
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpmI CTGGAG 2 cut(s) 947, 1446
Bpu14I TTCGAA 2 cut(s) 1038, 2170
BpuEI CTTGAG 2 cut(s) 65, 1999
BsaAI YACGTR 1 cut(s) 1191
BsaI GGTCTC 3 cut(s) 623, 761, 2185
BsaJI CCNNGG 3 cut(s) 53, 1449, 2195
BsaWI WCCGGW 2 cut(s) 770, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 4 cut(s) 1019, 1175, 1879, 1975
Bse1I ACTGG 2 cut(s) 823, 1429
BseBI CCWGG 1 cut(s) 1450
BseDI CCNNGG 3 cut(s) 53, 1449, 2195
BseGI GGATG 6 cut(s) 121, 172, 1169, 1307, 2057, 2131
BseLI CCNNNNNNNGG 4 cut(s) 1019, 1175, 1879, 1975
BseMII CTCAG 4 cut(s) 8, 387, 874, 1257
BseNI ACTGG 2 cut(s) 823, 1429
BseSI GKGCMC 4 cut(s) 368, 735, 1399, 2032
BseXI GCAGC 8 cut(s) 54, 1209, 1301, 1519, 1635, 1692, 2053, 2056
BseYI CCCAGC 2 cut(s) 342, 886
BsgI GTGCAG 1 cut(s) 1699
BshFI GGCC 2 cut(s) 1692, 2063
BshNI GGYRCC 2 cut(s) 1670, 1780
BsiHKAI GWGCWC 2 cut(s) 368, 1490
BsiHKCI CYCGRG 1 cut(s) 1900
BsiSI CCGG 2 cut(s) 771, 1560
BslFI GGGAC 4 cut(s) 19, 1646, 1843, 1917
BslI CCNNNNNNNGG 4 cut(s) 1019, 1175, 1879, 1975
BsmAI GTCTC 6 cut(s) 491, 623, 761, 798, 2185, 2194
BsmBI CGTCTC 2 cut(s) 491, 798
BsmFI GGGAC 4 cut(s) 19, 1646, 1843, 1917
BsnI GGCC 2 cut(s) 1692, 2063
Bso31I GGTCTC 3 cut(s) 623, 761, 2185
BsoBI CYCGRG 1 cut(s) 1900
Bsp119I TTCGAA 2 cut(s) 1038, 2170
Bsp1286I GDGCHC 5 cut(s) 368, 735, 1399, 1490, 2032
Bsp1407I TGTACA 1 cut(s) 326
Bsp143I GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
BspACI CCGC 5 cut(s) 65, 1136, 1651, 2064, 2144
BspANI GGCC 2 cut(s) 1692, 2063
BspCNI CTCAG 4 cut(s) 9, 388, 873, 1258
BspLI GGNNCC 4 cut(s) 1167, 1427, 1672, 1782
BspMAI CTGCAG 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 1227
BspPI GGATC 4 cut(s) 244, 506, 1160, 1173
BspQI GCTCTTC 1 cut(s) 1497
BspT104I TTCGAA 2 cut(s) 1038, 2170
BspT107I GGYRCC 2 cut(s) 1670, 1780
BspTNI GGTCTC 3 cut(s) 623, 761, 2185
BsrGI TGTACA 1 cut(s) 326
BsrI ACTGG 2 cut(s) 823, 1429
BssECI CCNNGG 3 cut(s) 53, 1449, 2195
BssMI GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
BssNAI GTATAC 1 cut(s) 224
BssSI CACGAG 1 cut(s) 1274
BssT1I CCWWGG 2 cut(s) 53, 2195
Bst1107I GTATAC 1 cut(s) 224
Bst2BI CACGAG 1 cut(s) 1274
Bst2UI CCWGG 1 cut(s) 1450
Bst4CI ACNGT 9 cut(s) 125, 502, 520, 577, 587, 842, 1035, 1974, 2231
Bst6I CTCTTC 1 cut(s) 1497
BstAPI GCANNNNNTGC 2 cut(s) 739, 2078
BstAUI TGTACA 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 1191
BstBI TTCGAA 2 cut(s) 1038, 2170
BstC8I GCNNGC 2 cut(s) 884, 1225
BstDEI CTNAG 7 cut(s) 17, 384, 396, 860, 1266, 1604, 1948
BstENI CCTNNNNNAGG 1 cut(s) 1017
BstF5I GGATG 6 cut(s) 121, 172, 1169, 1307, 2057, 2131
BstHHI GCGC 1 cut(s) 2121
BstKTI GATC 8 cut(s) 239, 514, 781, 1168, 1219, 1636, 1744, 1891
BstMAI GTCTC 6 cut(s) 491, 623, 761, 798, 2185, 2194
BstMBI GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
BstMWI GCNNNNNNNGC 8 cut(s) 739, 1289, 1445, 1454, 1463, 1689, 2069, 2078
BstNI CCWGG 1 cut(s) 1450
BstNSI RCATGY 5 cut(s) 161, 277, 327, 1720, 2026
BstSCI CCNGG 1 cut(s) 1448
BstSFI CTRYAG 3 cut(s) 40, 240, 337
BstSLI GKGCMC 4 cut(s) 368, 735, 1399, 2032
BstV1I GCAGC 8 cut(s) 54, 1209, 1301, 1519, 1635, 1692, 2053, 2056
BstV2I GAAGAC 3 cut(s) 1047, 1583, 2232
BstX2I RGATCY 5 cut(s) 236, 511, 1165, 1216, 1888
BstXI CCANNNNNNTGG 2 cut(s) 411, 886
BstYI RGATCY 5 cut(s) 236, 511, 1165, 1216, 1888
BstZ17I GTATAC 1 cut(s) 224
BsuRI GGCC 2 cut(s) 1692, 2063
BtsCI GGATG 6 cut(s) 121, 172, 1169, 1307, 2057, 2131
BtsI GCAGTG 2 cut(s) 270, 1566
BtsIMutI CAGTG 5 cut(s) 270, 525, 665, 847, 1566
Cac8I GCNNGC 2 cut(s) 884, 1225
CaiI CAGNNNCTG 2 cut(s) 28, 269
CfoI GCGC 1 cut(s) 2121
Csp6I GTAC 5 cut(s) 121, 327, 455, 578, 1522
CviQI GTAC 5 cut(s) 121, 327, 455, 578, 1522
DdeI CTNAG 7 cut(s) 17, 384, 396, 860, 1266, 1604, 1948
DpnI GATC 8 cut(s) 238, 513, 780, 1167, 1218, 1635, 1743, 1890
DpnII GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
DraI TTTAAA 2 cut(s) 205, 1372
EaeI YGGCCR 1 cut(s) 2061
Eam1104I CTCTTC 1 cut(s) 1497
EarI CTCTTC 1 cut(s) 1497
Eco130I CCWWGG 2 cut(s) 53, 2195
Eco31I GGTCTC 3 cut(s) 623, 761, 2185
Eco32I GATATC 1 cut(s) 248
Eco57I CTGAAG 5 cut(s) 269, 404, 528, 771, 1778
Eco88I CYCGRG 1 cut(s) 1900
EcoNI CCTNNNNNAGG 1 cut(s) 1017
EcoRII CCWGG 1 cut(s) 1448
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 53, 2195
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 53, 2195
Esp3I CGTCTC 2 cut(s) 491, 798
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 1825, 1857
FaqI GGGAC 4 cut(s) 19, 1646, 1843, 1917
FauI CCCGC 2 cut(s) 1644, 2137
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 1741
FblI GTMKAC 2 cut(s) 223, 722
Fnu4HI GCNGC 9 cut(s) 43, 1198, 1290, 1508, 1649, 1681, 2064, 2067, 2070
FokI GGATG 6 cut(s) 128, 179, 1156, 1294, 2064, 2118
Fsp4HI GCNGC 9 cut(s) 43, 1198, 1290, 1508, 1649, 1681, 2064, 2067, 2070
FspBI CTAG 7 cut(s) 260, 764, 941, 1203, 1224, 2196, 2296
GlaI GCGC 1 cut(s) 2120
GluI GCNGC 9 cut(s) 43, 1198, 1290, 1508, 1649, 1681, 2064, 2067, 2070
GsaI CCCAGC 2 cut(s) 346, 890
GsuI CTGGAG 2 cut(s) 947, 1446
HaeIII GGCC 2 cut(s) 1692, 2063
HapII CCGG 2 cut(s) 771, 1560
HhaI GCGC 1 cut(s) 2121
Hin6I GCGC 1 cut(s) 2119
HinP1I GCGC 1 cut(s) 2119
HincII GTYRAC 2 cut(s) 723, 1498
HindII GTYRAC 2 cut(s) 723, 1498
HinfI GANTC 7 cut(s) 374, 415, 543, 755, 848, 986, 1627
HpaII CCGG 2 cut(s) 771, 1560
HphI GGTGA 6 cut(s) 122, 326, 1087, 1333, 1429, 2198
Hpy166II GTNNAC 6 cut(s) 224, 366, 723, 857, 1109, 1498
Hpy188I TCNGA 9 cut(s) 18, 166, 195, 685, 704, 906, 1321, 1970, 2224
Hpy8I GTNNAC 6 cut(s) 224, 366, 723, 857, 1109, 1498
Hpy99I CGWCG 3 cut(s) 1974, 2188, 2191
HpyAV CCTTC 1 cut(s) 1987
HpyCH4III ACNGT 9 cut(s) 125, 502, 520, 577, 587, 842, 1035, 1974, 2231
HpyCH4IV ACGT 2 cut(s) 1044, 1190
HpyF10VI GCNNNNNNNGC 8 cut(s) 739, 1289, 1445, 1454, 1463, 1689, 2069, 2078
HpyF3I CTNAG 7 cut(s) 17, 384, 396, 860, 1266, 1604, 1948
HpySE526I ACGT 2 cut(s) 1044, 1190
HspAI GCGC 1 cut(s) 2119
Ksp22I TGATCA 1 cut(s) 1741
Kzo9I GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
LguI GCTCTTC 1 cut(s) 1497
LmnI GCTCC 1 cut(s) 1645
Lsp1109I GCAGC 8 cut(s) 54, 1209, 1301, 1519, 1635, 1692, 2053, 2056
LweI GCATC 8 cut(s) 170, 186, 1252, 1911, 2081, 2106, 2140, 2293
MaeI CTAG 7 cut(s) 260, 764, 941, 1203, 1224, 2196, 2296
MaeII ACGT 2 cut(s) 1044, 1190
MaeIII GTNAC 5 cut(s) 606, 652, 814, 962, 1417
MalI GATC 8 cut(s) 238, 513, 780, 1167, 1218, 1635, 1743, 1890
MboI GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
MfeI CAATTG 2 cut(s) 252, 1376
MflI RGATCY 5 cut(s) 236, 511, 1165, 1216, 1888
MhlI GDGCHC 5 cut(s) 368, 735, 1399, 1490, 2032
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 5 cut(s) 539, 997, 1831, 1956, 1993
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 4 cut(s) 414, 1082, 1411, 2093
MseI TTAA 8 cut(s) 204, 623, 660, 1047, 1329, 1371, 1727, 2289
MslI CAYNNNNRTG 5 cut(s) 90, 231, 561, 1478, 1919
MspA1I CMGCKG 1 cut(s) 2066
MspI CCGG 2 cut(s) 771, 1560
MspR9I CCNGG 1 cut(s) 1450
MunI CAATTG 2 cut(s) 252, 1376
MvaI CCWGG 1 cut(s) 1450
MwoI GCNNNNNNNGC 8 cut(s) 739, 1289, 1445, 1454, 1463, 1689, 2069, 2078
NdeI CATATG 1 cut(s) 469
NdeII GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
NheI GCTAGC 1 cut(s) 1223
NlaIV GGNNCC 4 cut(s) 1167, 1427, 1672, 1782
NmuCI GTSAC 3 cut(s) 606, 814, 1417
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 5 cut(s) 161, 277, 327, 1720, 2026
NspV TTCGAA 2 cut(s) 1038, 2170
OliI CACNNNNGTG 2 cut(s) 90, 561
PciI ACATGT 2 cut(s) 273, 2022
PciSI GCTCTTC 1 cut(s) 1497
PdmI GAANNNNTTC 4 cut(s) 414, 1082, 1411, 2093
PfeI GAWTC 6 cut(s) 415, 543, 755, 848, 986, 1627
PflMI CCANNNNNTGG 1 cut(s) 1175
PkrI GCNGC 9 cut(s) 44, 1199, 1291, 1509, 1650, 1682, 2065, 2068, 2071
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 1191
PscI ACATGT 2 cut(s) 273, 2022
Psp6I CCWGG 1 cut(s) 1448
PspFI CCCAGC 2 cut(s) 342, 886
PspGI CCWGG 1 cut(s) 1448
PspN4I GGNNCC 4 cut(s) 1167, 1427, 1672, 1782
PstI CTGCAG 1 cut(s) 44
PstNI CAGNNNCTG 2 cut(s) 28, 269
PsuI RGATCY 5 cut(s) 236, 511, 1165, 1216, 1888
RsaI GTAC 5 cut(s) 122, 328, 456, 579, 1523
RsaNI GTAC 5 cut(s) 121, 327, 455, 578, 1522
RseI CAYNNNNRTG 5 cut(s) 90, 231, 561, 1478, 1919
SalI GTCGAC 1 cut(s) 721
SapI GCTCTTC 1 cut(s) 1497
SaqAI TTAA 8 cut(s) 204, 623, 660, 1047, 1329, 1371, 1727, 2289
SatI GCNGC 9 cut(s) 43, 1198, 1290, 1508, 1649, 1681, 2064, 2067, 2070
Sau3AI GATC 8 cut(s) 236, 511, 778, 1165, 1216, 1633, 1741, 1888
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 1450
SduI GDGCHC 5 cut(s) 368, 735, 1399, 1490, 2032
SfaNI GCATC 8 cut(s) 170, 186, 1252, 1911, 2081, 2106, 2140, 2293
SfcI CTRYAG 3 cut(s) 40, 240, 337
SfuI TTCGAA 2 cut(s) 1038, 2170
SmiMI CAYNNNNRTG 5 cut(s) 90, 231, 561, 1478, 1919
SmlI CTYRAG 2 cut(s) 80, 2014
SmoI CTYRAG 2 cut(s) 80, 2014
SsiI CCGC 5 cut(s) 65, 1136, 1651, 2064, 2144
SspI AATATT 2 cut(s) 1327, 2161
SspMI CTAG 7 cut(s) 260, 764, 941, 1203, 1224, 2196, 2296
StyD4I CCNGG 1 cut(s) 1448
StyI CCWWGG 2 cut(s) 53, 2195
TaaI ACNGT 9 cut(s) 125, 502, 520, 577, 587, 842, 1035, 1974, 2231
TaiI ACGT 2 cut(s) 1047, 1193
TaqI TCGA 4 cut(s) 722, 869, 1038, 2170
TaqII GACCGA 1 cut(s) 1716
TatI WGTACW 1 cut(s) 326
TauI GCSGC 1 cut(s) 2066
TfiI GAWTC 6 cut(s) 415, 543, 755, 848, 986, 1627
Tru1I TTAA 8 cut(s) 204, 623, 660, 1047, 1329, 1371, 1727, 2289
Tru9I TTAA 8 cut(s) 204, 623, 660, 1047, 1329, 1371, 1727, 2289
TscAI CASTG 5 cut(s) 270, 525, 672, 847, 1573
TseFI GTSAC 3 cut(s) 606, 814, 1417
TseI GCWGC 8 cut(s) 42, 1197, 1289, 1507, 1648, 1680, 2066, 2069
Tsp45I GTSAC 3 cut(s) 606, 814, 1417
TspDTI ATGAA 5 cut(s) 417, 423, 1314, 1947, 2289
TspRI CASTG 5 cut(s) 270, 525, 672, 847, 1573
Van91I CCANNNNNTGG 1 cut(s) 1175
VneI GTGCAC 1 cut(s) 364
XagI CCTNNNNNAGG 1 cut(s) 1017
XapI RAATTY 3 cut(s) 619, 1156, 1382
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 5 cut(s) 161, 277, 327, 1720, 2026
XmaJI CCTAGG 1 cut(s) 2195
XmiI GTMKAC 2 cut(s) 223, 722
XmnI GAANNNNTTC 4 cut(s) 414, 1082, 1411, 2093
XspI CTAG 7 cut(s) 260, 764, 941, 1203, 1224, 2196, 2296
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.