MD12G1149300.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012635
Physical Location & Seq
Reverse (-)
22911220 .. 22915459
4240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1149300.v1.1.491

Sequence Viewer

Length: 2007 bp
ATGGGGACTACTGCTCTGAGGCAGTTGCTGAAGAGCCTCTGCAGTGATTCGCCTTGGAATTATTCAGTGTTTTGGAAGCTCAAGCACCAGAGTGATTTGATTTTGTCTTGGGAGGATGGGTACTGTCATCACCCAAATCCAAGAGAAACTGTGGAACATGCATCGGATGACATCTACTTCAGCGACGCAAATCAGATGCATTTTAAGGATTTTGCAACAAGTATCCATGATGGTGGATCTGCAGGATATCCAATTGGACTGGCTGTGGCTGATATGGTACATCTTCAGTACACATTTGGAAAAGGGGTTGTTGGTGAGGTGGCATGTACAGGGAGCTTTACCTGGGTTTTCCTTGACAGTCTATGCACCAGAGAGTCTGATTTAGTTCCTCAGTGTCCAGATGAATGGCTTCTTCAGTTTGCATTGGGTATCAAGACAATTTTGCTTGTACCTGTACTTCCATATGGAGTTCTGCAACTTGGCTCCCTGGAGACGGTTGCTGAAGATCCTGCAGTGGTTGCTTTTGTGAAAGATAGATTCAATTCTATTCACAATGTTGAGGGGAAAACTGTACCTTTTACCTTAAACAAAGACATTGAAGCTCAATCATCATGGTCACAATCATTTAGTTTAATGCAGAACACATTTGAGCCATCAGCTGTTACCATGAATCCACTCAACGTTGAAGGGTCAGAAGATGTTCATAACATCAGACTGAATGATAACTTATTGTCGACCTTCGGGCAATTTTTGCAATTGCCAACTGCTGAAGATTCACTTCTAGAGACCGGAACAGACGAGCCAGAAGTTCTTAAGAGTACAGGCGAAAGTGTGATTGCTGTTCCGCCAACTTTTCACTGTGGAGAATCAAGTCCACTCAGTCAGTCGATAGATACCAGCTTGGGTATGATGGAAAGTCAGATGTTTGGGCTGTCTTGTCTAGAGGAAGAACTTCTAGCCTATTCTCAATTTGGTGGCTATGGTTTGGAAGTGTTTGGAGAATCCATGAGCGGTTTCAATTCTTACTCTGTTGGAGGTGTAGCAGAACTGTTCGAAGACGTTAATGCTAAAGATACAAGTTACAACACAGTGAATAATTTCTTTAGCTTTCCTGAAAATTGTGAACTACACAAAGCACTTGGAACACCTTTGCAGAGGCAGACAGATGAACATATATGGGACCCATCTATCGGCATTGATGATACATGTAGCAGCTCTAGTTTGCAGAAAGATCTCCCTACCCGTATTGAGCCATCATGGTTTTCCAAAGCAAGTGATGCTGAGAACCTCTCGAAAGCTTTTGTAGCCAAAGATGAAACCTCATCTAGCAGATCAGAGAATATTAAATCATCTATTACCTCATCAAGTCAACTTCCTGCTTCTTTCAAACAATTGAAATTTGAAGCGAGTGCCCCAGTTGGAAGTGATTCAGCAACATGGAACCATACATCTGCTTTACCTGCCAGGGGCGAGATTTCAACTTCTTTCACAGGCACGATAAGTACTTTGGTTGACGAAGAGCAGAAGGAGAAAGGGTACAAGTCTACAAAGCCTAAAAAGGAACAAAAGTTGTCCGGTGGCACTGCAAGAAAGACCAGGCTTGGCAATAGCCGGAAGCTAAGACCAAGAGATAGACAACTGATTCAGGATCGTATGAAAGAGCTACGAGACCTTGTCCCAAATGGTGCTAAGTGCAGCATTGACGGCCTTTTAGACCGAACCGTTAAACACATGCAGTTTTTAAGAAACATGACTGACCAAACCGAAAAACTGAGGTGCTACACACCACAAGAGGCACCTCATTCCAATAACACAAGCGAAGCCACAAGTGGCTGCGAAAGTGGGACAAGCAGGGCTTTCGAAGTTGGAAGTGAACTCCAGATTTGCCCCATTGTGGTAGAAGATCTTGAACACCCAGGACACATGCTTATAGAGGTTTGTTGCATGCCCCCCTATTTGTCAACGCGAAGCTTCGTTGCAGAAGCGTTTCGTCTAAAATTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bHLH-MYC_N

Protein Analysis

669

Amino Acids

73.73

Weight (kDa)

4.92

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 6 - 180 2e-31 bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_LHW PF23176 540 - 591 3.9e-26 Transcription factor LHW bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13090 FvH4_6g13090 FvH4_6g13090 FvH4_6g13090 FvH4_6g13090
malus_domestica MD04G1135600.v1.1 MD12G1149300.v1.1
prunus_persica Prupe.6G260900_v2.0.a1
pyrus_communis pycom04g12270 pycom12g14310
rosa_chinensis RchiOBHm_Chr3g0465361
rosa_laevigata RLG00000024643
rosa_multiflora Rmu_sc0011781.1_g000003
rosa_roxburghii Rroxscaffold_6G00415100
rosa_rugosa Rorug03G0076300
rosa_samantha Rh3BG141300 Rh3CG142400 Rh3DG142400
rosa_wichuraiana Rw3G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1468
AccB1I GGYRCC 1 cut(s) 1795
AccBSI CCGCTC 1 cut(s) 1011
AccI GTMKAC 2 cut(s) 734, 1544
AccII CGCG 1 cut(s) 1966
AciI CCGC 2 cut(s) 845, 1011
AclI AACGTT 1 cut(s) 681
AclWI GGATC 3 cut(s) 244, 500, 1656
AcsI RAATTY 2 cut(s) 1397, 1997
AcuI CTGAAG 6 cut(s) 50, 163, 269, 398, 522, 789
AfiI CCNNNNNNNGG 4 cut(s) 493, 1190, 1466, 1894
AflII CTTAAG 1 cut(s) 812
AflIII ACRYGT 1 cut(s) 1205
AgsI TTSAA 9 cut(s) 541, 599, 686, 1018, 1387, 1396, 1403, 1479, 1910
AjnI CCWGG 5 cut(s) 341, 486, 1463, 1595, 1915
AleI CACNNNNGTG 1 cut(s) 90
Alw26I GTCTC 3 cut(s) 485, 779, 1662
AlwI GGATC 3 cut(s) 244, 500, 1656
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 1 cut(s) 1705
ApeKI GCWGC 3 cut(s) 1212, 1695, 1833
ApoI RAATTY 2 cut(s) 1397, 1997
Asp700I GAANNNNTTC 6 cut(s) 408, 699, 951, 1097, 1426, 1986
AspS9I GGNCC 1 cut(s) 1180
AsuHPI GGTGA 2 cut(s) 122, 326
AsuII TTCGAA 2 cut(s) 1053, 1860
AvaII GGWCC 1 cut(s) 1180
BaeGI GKGCMC 1 cut(s) 1414
BanI GGYRCC 1 cut(s) 1795
BarI GAAGNNNNNNTAC 2 cut(s) 441, 473
BbsI GAAGAC 1 cut(s) 1062
BbvI GCAGC 3 cut(s) 1224, 1707, 1820
BccI CCATC 6 cut(s) 110, 224, 661, 904, 1192, 1261
BceAI ACGGC 1 cut(s) 1720
BcgI CGANNNNNNTGC 2 cut(s) 1840, 1874
BciT130I CCWGG 5 cut(s) 343, 488, 1465, 1597, 1917
BciVI GTATCC 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 485, 779, 1662
BfaI CTAG 5 cut(s) 782, 941, 956, 1218, 1326
BfmI CTRYAG 3 cut(s) 40, 240, 510
BfrI CTTAAG 1 cut(s) 812
BfuAI ACCTGC 1 cut(s) 1468
BfuI GTATCC 1 cut(s) 233
BglII AGATCT 2 cut(s) 1231, 1903
BisI GCNGC 3 cut(s) 1213, 1696, 1834
BlsI GCNGC 3 cut(s) 1214, 1697, 1835
BmcAI AGTACT 1 cut(s) 1504
Bme1390I CCNGG 5 cut(s) 343, 488, 1465, 1597, 1917
Bme18I GGWCC 1 cut(s) 1180
BmgT120I GGNCC 1 cut(s) 1180
BmiI GGNNCC 5 cut(s) 484, 1181, 1182, 1442, 1797
BmrFI CCNGG 5 cut(s) 343, 488, 1465, 1597, 1917
BmrI ACTGGG 1 cut(s) 1409
BmsI GCATC 3 cut(s) 170, 186, 1267
BmuI ACTGGG 1 cut(s) 1409
BpiI GAAGAC 1 cut(s) 1062
BplI GAGNNNNNCTC 2 cut(s) 1274, 1306
BpmI CTGGAG 2 cut(s) 509, 1862
Bpu14I TTCGAA 2 cut(s) 1053, 1860
BpuEI CTTGAG 1 cut(s) 65
BsaI GGTCTC 2 cut(s) 779, 1662
BsaJI CCNNGG 5 cut(s) 53, 342, 486, 1464, 1915
BsaWI WCCGGW 2 cut(s) 788, 1574
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 1520, 1550
Bsc4I CCNNNNNNNGG 4 cut(s) 493, 1190, 1466, 1894
Bse1I ACTGG 2 cut(s) 264, 1415
BseBI CCWGG 5 cut(s) 343, 488, 1465, 1597, 1917
BseDI CCNNGG 5 cut(s) 53, 342, 486, 1464, 1915
BseGI GGATG 2 cut(s) 121, 172
BseLI CCNNNNNNNGG 4 cut(s) 493, 1190, 1466, 1894
BseMII CTCAG 5 cut(s) 8, 404, 892, 1272, 1763
BseNI ACTGG 2 cut(s) 264, 1415
BseSI GKGCMC 1 cut(s) 1414
BseXI GCAGC 3 cut(s) 1224, 1707, 1820
BsgI GTGCAG 1 cut(s) 1714
Bsh1236I CGCG 1 cut(s) 1966
BshFI GGCC 1 cut(s) 1707
BshNI GGYRCC 1 cut(s) 1795
BsiSI CCGG 3 cut(s) 789, 1575, 1612
BslFI GGGAC 4 cut(s) 19, 1193, 1661, 1858
BslI CCNNNNNNNGG 4 cut(s) 493, 1190, 1466, 1894
BsmAI GTCTC 3 cut(s) 485, 779, 1662
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 4 cut(s) 19, 1193, 1661, 1858
BsnI GGCC 1 cut(s) 1707
Bso31I GGTCTC 2 cut(s) 779, 1662
Bsp119I TTCGAA 2 cut(s) 1053, 1860
Bsp1286I GDGCHC 1 cut(s) 1414
Bsp1407I TGTACA 1 cut(s) 326
Bsp143I GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
BspACI CCGC 2 cut(s) 845, 1011
BspANI GGCC 1 cut(s) 1707
BspCNI CTCAG 5 cut(s) 9, 403, 891, 1273, 1764
BspFNI CGCG 1 cut(s) 1966
BspLI GGNNCC 5 cut(s) 484, 1181, 1182, 1442, 1797
BspMAI CTGCAG 3 cut(s) 44, 244, 514
BspMI ACCTGC 1 cut(s) 1468
BspPI GGATC 3 cut(s) 244, 500, 1656
BspQI GCTCTTC 2 cut(s) 26, 1512
BspT104I TTCGAA 2 cut(s) 1053, 1860
BspT107I GGYRCC 1 cut(s) 1795
BspTI CTTAAG 1 cut(s) 812
BspTNI GGTCTC 2 cut(s) 779, 1662
BsrBI CCGCTC 1 cut(s) 1011
BsrGI TGTACA 1 cut(s) 326
BsrI ACTGG 2 cut(s) 264, 1415
BssECI CCNNGG 5 cut(s) 53, 342, 486, 1464, 1915
BssMI GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 5 cut(s) 343, 488, 1465, 1597, 1917
Bst4CI ACNGT 9 cut(s) 125, 151, 359, 496, 571, 860, 1050, 1090, 1723
Bst6I CTCTTC 2 cut(s) 26, 1512
BstAFI CTTAAG 1 cut(s) 812
BstAPI GCANNNNNTGC 3 cut(s) 518, 751, 1277
BstAUI TGTACA 1 cut(s) 326
BstBI TTCGAA 2 cut(s) 1053, 1860
BstC8I GCNNGC 1 cut(s) 1946
BstDEI CTNAG 7 cut(s) 17, 390, 878, 1281, 1619, 1689, 1772
BstF5I GGATG 2 cut(s) 121, 172
BstFNI CGCG 1 cut(s) 1966
BstKTI GATC 6 cut(s) 239, 508, 1234, 1334, 1651, 1906
BstMAI GTCTC 3 cut(s) 485, 779, 1662
BstMBI GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
BstMWI GCNNNNNNNGC 6 cut(s) 518, 751, 1277, 1304, 1460, 1704
BstNI CCWGG 5 cut(s) 343, 488, 1465, 1597, 1917
BstNSI RCATGY 6 cut(s) 161, 327, 1209, 1735, 1927, 1948
BstSCI CCNGG 5 cut(s) 341, 486, 1463, 1595, 1915
BstSFI CTRYAG 3 cut(s) 40, 240, 510
BstSLI GKGCMC 1 cut(s) 1414
BstUI CGCG 1 cut(s) 1966
BstV1I GCAGC 3 cut(s) 1224, 1707, 1820
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 4 cut(s) 236, 505, 1231, 1903
BstXI CCANNNNNNTGG 2 cut(s) 233, 405
BstYI RGATCY 4 cut(s) 236, 505, 1231, 1903
BsuI GTATCC 1 cut(s) 233
BsuRI GGCC 1 cut(s) 1707
BtsCI GGATG 2 cut(s) 121, 172
BtsI GCAGTG 3 cut(s) 49, 519, 1581
BtsIMutI CAGTG 7 cut(s) 49, 72, 398, 519, 856, 1095, 1581
BveI ACCTGC 1 cut(s) 1468
Cac8I GCNNGC 1 cut(s) 1946
CaiI CAGNNNCTG 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 1180
CseI GACGC 1 cut(s) 194
DdeI CTNAG 7 cut(s) 17, 390, 878, 1281, 1619, 1689, 1772
DpnI GATC 6 cut(s) 238, 507, 1233, 1333, 1650, 1905
DpnII GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
Eam1104I CTCTTC 2 cut(s) 26, 1512
EarI CTCTTC 2 cut(s) 26, 1512
EciI GGCGGA 1 cut(s) 834
Eco130I CCWWGG 1 cut(s) 53
Eco31I GGTCTC 2 cut(s) 779, 1662
Eco32I GATATC 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 1180
Eco57I CTGAAG 6 cut(s) 50, 163, 269, 398, 522, 789
EcoO109I RGGNCCY 1 cut(s) 1180
EcoRII CCWGG 5 cut(s) 341, 486, 1463, 1595, 1915
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 2 cut(s) 163, 201
ErhI CCWWGG 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 485
FalI AAGNNNNNCTT 4 cut(s) 762, 794, 1840, 1872
FaqI GGGAC 4 cut(s) 19, 1193, 1661, 1858
FauNDI CATATG 1 cut(s) 463
FblI GTMKAC 2 cut(s) 734, 1544
Fnu4HI GCNGC 3 cut(s) 1213, 1696, 1834
FokI GGATG 2 cut(s) 128, 179
Fsp4HI GCNGC 3 cut(s) 1213, 1696, 1834
FspBI CTAG 5 cut(s) 782, 941, 956, 1218, 1326
GluI GCNGC 3 cut(s) 1213, 1696, 1834
GsuI CTGGAG 2 cut(s) 509, 1862
HaeIII GGCC 1 cut(s) 1707
HapII CCGG 3 cut(s) 789, 1575, 1612
HgaI GACGC 1 cut(s) 194
HincII GTYRAC 4 cut(s) 735, 1370, 1513, 1962
HindII GTYRAC 4 cut(s) 735, 1370, 1513, 1962
HindIII AAGCTT 2 cut(s) 1296, 1969
HinfI GANTC 9 cut(s) 47, 374, 537, 670, 773, 866, 1001, 1427, 1642
HpaII CCGG 3 cut(s) 789, 1575, 1612
HphI GGTGA 2 cut(s) 122, 326
Hpy166II GTNNAC 9 cut(s) 291, 735, 875, 1124, 1370, 1513, 1545, 1874, 1962
Hpy188I TCNGA 8 cut(s) 18, 166, 195, 379, 694, 713, 921, 1336
Hpy188III TCNNGA 9 cut(s) 398, 433, 782, 941, 1112, 1291, 1646, 1879, 1907
Hpy8I GTNNAC 9 cut(s) 291, 735, 875, 1124, 1370, 1513, 1545, 1874, 1962
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 680, 748, 1519
HpyCH4III ACNGT 9 cut(s) 125, 151, 359, 496, 571, 860, 1050, 1090, 1723
HpyCH4IV ACGT 2 cut(s) 681, 1059
HpyF10VI GCNNNNNNNGC 6 cut(s) 518, 751, 1277, 1304, 1460, 1704
HpyF3I CTNAG 7 cut(s) 17, 390, 878, 1281, 1619, 1689, 1772
HpySE526I ACGT 2 cut(s) 681, 1059
KflI GGGWCCC 1 cut(s) 1180
Kzo9I GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
LguI GCTCTTC 2 cut(s) 26, 1512
LmnI GCTCC 2 cut(s) 333, 488
Lsp1109I GCAGC 3 cut(s) 1224, 1707, 1820
LweI GCATC 3 cut(s) 170, 186, 1267
MaeI CTAG 5 cut(s) 782, 941, 956, 1218, 1326
MaeII ACGT 2 cut(s) 681, 1059
MaeIII GTNAC 3 cut(s) 615, 661, 1079
MalI GATC 6 cut(s) 238, 507, 1233, 1333, 1650, 1905
MbiI CCGCTC 1 cut(s) 1011
MboI GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
MfeI CAATTG 3 cut(s) 252, 755, 1391
MflI RGATCY 4 cut(s) 236, 505, 1231, 1903
MhlI GDGCHC 1 cut(s) 1414
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 3 cut(s) 1012, 1399, 1846
Mph1103I ATGCAT 2 cut(s) 163, 201
MroXI GAANNNNTTC 6 cut(s) 408, 699, 951, 1097, 1426, 1986
MseI TTAA 8 cut(s) 204, 584, 632, 813, 1062, 1344, 1725, 1742
MslI CAYNNNNRTG 2 cut(s) 90, 231
MspA1I CMGCKG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 812
MspI CCGG 3 cut(s) 789, 1575, 1612
MspR9I CCNGG 5 cut(s) 343, 488, 1465, 1597, 1917
MunI CAATTG 3 cut(s) 252, 755, 1391
MvaI CCWGG 5 cut(s) 343, 488, 1465, 1597, 1917
MvnI CGCG 1 cut(s) 1966
MwoI GCNNNNNNNGC 6 cut(s) 518, 751, 1277, 1304, 1460, 1704
NdeI CATATG 1 cut(s) 463
NdeII GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
NlaIV GGNNCC 5 cut(s) 484, 1181, 1182, 1442, 1797
NmuCI GTSAC 1 cut(s) 615
NsiI ATGCAT 2 cut(s) 163, 201
NspI RCATGY 6 cut(s) 161, 327, 1209, 1735, 1927, 1948
NspV TTCGAA 2 cut(s) 1053, 1860
OliI CACNNNNGTG 1 cut(s) 90
PaeI GCATGC 1 cut(s) 1948
PciI ACATGT 1 cut(s) 1205
PciSI GCTCTTC 2 cut(s) 26, 1512
PdmI GAANNNNTTC 6 cut(s) 408, 699, 951, 1097, 1426, 1986
PfeI GAWTC 8 cut(s) 47, 537, 670, 773, 866, 1001, 1427, 1642
PflFI GACNNNGTC 1 cut(s) 1673
PkrI GCNGC 3 cut(s) 1214, 1697, 1835
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 1180
PscI ACATGT 1 cut(s) 1205
Psp1406I AACGTT 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 1180
Psp6I CCWGG 5 cut(s) 341, 486, 1463, 1595, 1915
PspGI CCWGG 5 cut(s) 341, 486, 1463, 1595, 1915
PspN4I GGNNCC 5 cut(s) 484, 1181, 1182, 1442, 1797
PspPI GGNCC 1 cut(s) 1180
PspPPI RGGWCCY 1 cut(s) 1180
PstI CTGCAG 3 cut(s) 44, 244, 514
PstNI CAGNNNCTG 1 cut(s) 28
PsuI RGATCY 4 cut(s) 236, 505, 1231, 1903
PsyI GACNNNGTC 1 cut(s) 1673
PvuII CAGCTG 1 cut(s) 659
RseI CAYNNNNRTG 2 cut(s) 90, 231
SalI GTCGAC 1 cut(s) 733
SapI GCTCTTC 2 cut(s) 26, 1512
SaqAI TTAA 8 cut(s) 204, 584, 632, 813, 1062, 1344, 1725, 1742
SatI GCNGC 3 cut(s) 1213, 1696, 1834
Sau3AI GATC 6 cut(s) 236, 505, 1231, 1331, 1648, 1903
Sau96I GGNCC 1 cut(s) 1180
ScaI AGTACT 1 cut(s) 1504
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 5 cut(s) 343, 488, 1465, 1597, 1917
SduI GDGCHC 1 cut(s) 1414
SfaNI GCATC 3 cut(s) 170, 186, 1267
SfcI CTRYAG 3 cut(s) 40, 240, 510
SfuI TTCGAA 2 cut(s) 1053, 1860
SinI GGWCC 1 cut(s) 1180
SmiMI CAYNNNNRTG 2 cut(s) 90, 231
SmlI CTYRAG 2 cut(s) 80, 812
SmoI CTYRAG 2 cut(s) 80, 812
SphI GCATGC 1 cut(s) 1948
SsiI CCGC 2 cut(s) 845, 1011
SspI AATATT 1 cut(s) 1342
SspMI CTAG 5 cut(s) 782, 941, 956, 1218, 1326
StyD4I CCNGG 5 cut(s) 341, 486, 1463, 1595, 1915
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 9 cut(s) 125, 151, 359, 496, 571, 860, 1050, 1090, 1723
TaiI ACGT 2 cut(s) 684, 1062
TaqI TCGA 5 cut(s) 734, 887, 1053, 1292, 1860
TaqII GACCGA 1 cut(s) 1731
TatI WGTACW 5 cut(s) 288, 326, 454, 818, 1502
TfiI GAWTC 8 cut(s) 47, 537, 670, 773, 866, 1001, 1427, 1642
Tru1I TTAA 8 cut(s) 204, 584, 632, 813, 1062, 1344, 1725, 1742
Tru9I TTAA 8 cut(s) 204, 584, 632, 813, 1062, 1344, 1725, 1742
TscAI CASTG 7 cut(s) 49, 72, 398, 519, 863, 1095, 1588
TseFI GTSAC 1 cut(s) 615
TseI GCWGC 3 cut(s) 1212, 1695, 1833
Tsp45I GTSAC 1 cut(s) 615
TspDTI ATGAA 6 cut(s) 417, 683, 692, 1182, 1329, 1670
TspRI CASTG 7 cut(s) 49, 72, 398, 519, 863, 1095, 1588
Tth111I GACNNNGTC 1 cut(s) 1673
Vha464I CTTAAG 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 1180
XapI RAATTY 2 cut(s) 1397, 1997
XbaI TCTAGA 2 cut(s) 781, 940
XceI RCATGY 6 cut(s) 161, 327, 1209, 1735, 1927, 1948
XmiI GTMKAC 2 cut(s) 734, 1544
XmnI GAANNNNTTC 6 cut(s) 408, 699, 951, 1097, 1426, 1986
XspI CTAG 5 cut(s) 782, 941, 956, 1218, 1326
ZrmI AGTACT 1 cut(s) 1504
Zsp2I ATGCAT 2 cut(s) 163, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.