MD04G1147200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013532
Physical Location & Seq
Forward (+)
23557770 .. 23558594
825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1147200.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGTCGACTTCCCCAAATCTCTCTCCCCGAAGCGAGTCCTGAAGCTCCTCAAAGCCGAGAAGAATCCTCACGCCGCATTCGCACTGCTCGACTCAGTGACCCGCCACCCGAAGTACAACCACTCCCCCGATAAAGCTATGGCCGTGTTTCAACAGATGGAAGAGATTTTCGGGTGTGCGCCCGGCGTAAGGTCGTACAATTCTCTGCTGAATGCATTCATTGAGTCCAATCAGCAGGACCGAGCTGAGAAGTTTTTCGCCTATTTCGAAACAGTTGGTTTGGAGCCGAATTGGCAGACTTATAATGTTCTGATCAAGATTTCGTGCAAGAAGAAGCATTTTGAGAAGGCCAAAGGGTTGCTGAACTGGATGTGGGAAAAGGGTTTGGAGCCTGATGTGTTTGGTTATGGGGCTTTGATCAACGGGCTGGCGAAAGGTGGAAACTTGAGTGATGCATTGGACGTGTTTGACGAAAAGCTTGAGAGAGGGGACTCAGAGGTTTATCCTAATGTCGTTACTTATAATGTTGTGGTCAATGGTCTGTGCAAATGCGGGAAGGTCAATGAGAGTTTGGAGATATGGAGTGGGATGAAGACAAATGACAGAGGGCCCGATTTTTTTTTTTTTACTTGTAGTTCTTTGATTCGACATTCACCTCTCCAAGACCCTGCGTAA

Protein Analysis

225

Amino Acids

25.29

Weight (kDa)

6.95

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 27 - 110 1.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 61 - 110 8.8e-10 PPR repeat family
PPR_3 PF13812 62 - 107 3.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 100 - 145 3.3e-12 PPR repeat family
PPR_3 PF13812 122 - 179 6.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 127 - 158 2.1e-08 PPR repeat
PPR_1 PF12854 167 - 198 6.2e-10 PPR repeat
PPR_2 PF13041 169 - 207 1.2e-08 PPR repeat family
PPR PF01535 172 - 200 1.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013532)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09060 AT3G09060
fragaria_vesca FvH4_6g11981
malus_domestica MD04G1147200.v1.1 MD12G1160500.v1.1
prunus_persica Prupe.6G271000_v2.0.a1
pyrus_communis pycom12g15230
rosa_chinensis RchiOBHm_Chr3g0463821
rosa_laevigata RLG00000024754
rosa_multiflora Rmu_sc0004796.1_g000010
rosa_roxburghii Rroxscaffold_6G00416270
rosa_rugosa Rorug03G0068000 Rorug03G0068100
rosa_samantha Rh3AG125000 Rh3BG129300 Rh3CG131900 Rh3DG130400
rosa_wichuraiana Rw3G010560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 303, 522
AccI GTMKAC 1 cut(s) 6
AciI CCGC 3 cut(s) 75, 103, 552
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 62
AfaI GTAC 2 cut(s) 116, 197
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 1 cut(s) 152
AjiI CACGTC 1 cut(s) 463
AluBI AGCT 4 cut(s) 46, 137, 245, 478
AluI AGCT 4 cut(s) 46, 137, 245, 478
AoxI GGCC 3 cut(s) 141, 348, 608
ApaI GGGCCC 1 cut(s) 612
Asp700I GAANNNNTTC 2 cut(s) 215, 254
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 238, 608, 609
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 645
AsuII TTCGAA 1 cut(s) 267
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 612
BanII GRGCYC 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 599
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 128
BclI TGATCA 2 cut(s) 312, 417
BcnI CCSGG 1 cut(s) 183
BglI GCCNNNNNGGC 1 cut(s) 292
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 238, 608, 609
BmiI GGNNCC 3 cut(s) 285, 390, 610
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 599
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 466, 500
BpuMI CCSGG 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 371
BseGI GGATG 2 cut(s) 375, 594
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 3 cut(s) 108, 237, 507
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 612
BshFI GGCC 3 cut(s) 143, 350, 610
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 3 cut(s) 77, 215, 217
BsnI GGCC 3 cut(s) 143, 350, 610
Bsp119I TTCGAA 1 cut(s) 267
Bsp120I GGGCCC 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 612
Bsp143I GATC 2 cut(s) 312, 417
BspACI CCGC 3 cut(s) 75, 103, 552
BspANI GGCC 3 cut(s) 143, 350, 610
BspCNI CTCAG 3 cut(s) 107, 238, 506
BspLI GGNNCC 3 cut(s) 285, 390, 610
BspT104I TTCGAA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 371
BssMI GATC 2 cut(s) 312, 417
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 156
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 94, 246, 493
BstF5I GGATG 2 cut(s) 375, 594
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 2 cut(s) 315, 420
BstMBI GATC 2 cut(s) 312, 417
BstMWI GCNNNNNNNGC 2 cut(s) 80, 292
BstSCI CCNGG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 612
BstV2I GAAGAC 1 cut(s) 599
BsuRI GGCC 3 cut(s) 143, 350, 610
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 375, 594
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 83, 102
Cac8I GCNNGC 1 cut(s) 429
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 3 cut(s) 238, 608, 609
Csp6I GTAC 2 cut(s) 115, 196
CviQI GTAC 2 cut(s) 115, 196
DdeI CTNAG 3 cut(s) 94, 246, 493
DpnI GATC 2 cut(s) 314, 419
DpnII GATC 2 cut(s) 312, 417
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 608
EcoT22I ATGCAT 2 cut(s) 217, 457
EcoT38I GRGCYC 1 cut(s) 612
FaiI YATR 5 cut(s) 140, 303, 408, 522, 580
FaqI GGGAC 1 cut(s) 503
FauI CCCGC 2 cut(s) 110, 545
FbaI TGATCA 2 cut(s) 312, 417
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 2 cut(s) 382, 601
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 1 cut(s) 75
GlaI GCGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 3 cut(s) 143, 350, 610
HapII CCGG 1 cut(s) 183
HhaI GCGC 1 cut(s) 181
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 6 cut(s) 36, 64, 92, 224, 491, 643
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 645
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 312, 496
Hpy188III TCNNGA 2 cut(s) 40, 316
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 2 cut(s) 340, 550
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 4 cut(s) 215, 327, 455, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 292
HpyF3I CTNAG 3 cut(s) 94, 246, 493
HpySE526I ACGT 1 cut(s) 462
HspAI GCGC 1 cut(s) 179
Ksp22I TGATCA 2 cut(s) 312, 417
Kzo9I GATC 2 cut(s) 312, 417
LmnI GCTCC 3 cut(s) 51, 283, 388
LpnPI CCDG 6 cut(s) 53, 196, 221, 352, 405, 413
LweI GCATC 1 cut(s) 442
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 97, 514
MalI GATC 2 cut(s) 314, 419
MboI GATC 2 cut(s) 312, 417
MboII GAAGA 4 cut(s) 73, 173, 343, 604
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 2 cut(s) 199, 289
MlyI GAGTC 4 cut(s) 45, 86, 233, 485
MnlI CCTC 6 cut(s) 59, 78, 479, 490, 599, 666
Mph1103I ATGCAT 2 cut(s) 217, 457
MroXI GAANNNNTTC 2 cut(s) 215, 254
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 183
Mva1269I GAATGC 3 cut(s) 77, 215, 217
MwoI GCNNNNNNNGC 2 cut(s) 80, 292
NciI CCSGG 1 cut(s) 183
NdeII GATC 2 cut(s) 312, 417
NlaIV GGNNCC 3 cut(s) 285, 390, 610
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 97
NsiI ATGCAT 2 cut(s) 217, 457
NspV TTCGAA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 264, 468
PctI GAATGC 3 cut(s) 77, 215, 217
PdmI GAANNNNTTC 2 cut(s) 215, 254
PfeI GAWTC 2 cut(s) 64, 643
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 4 cut(s) 44, 86, 232, 485
PpsI GAGTC 4 cut(s) 44, 86, 232, 485
PsiI TTATAA 2 cut(s) 303, 522
PspN4I GGNNCC 3 cut(s) 285, 390, 610
PspOMI GGGCCC 1 cut(s) 608
PspPI GGNCC 3 cut(s) 238, 608, 609
RsaI GTAC 2 cut(s) 116, 197
RsaNI GTAC 2 cut(s) 115, 196
SalI GTCGAC 1 cut(s) 5
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 2 cut(s) 312, 417
Sau96I GGNCC 3 cut(s) 238, 608, 609
SchI GAGTC 4 cut(s) 45, 86, 233, 485
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 1 cut(s) 442
SfuI TTCGAA 1 cut(s) 267
SinI GGWCC 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 445, 479
SmoI CTYRAG 2 cut(s) 445, 479
Sse9I AATT 2 cut(s) 199, 289
SsiI CCGC 3 cut(s) 75, 103, 552
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 465
TaqI TCGA 4 cut(s) 6, 90, 267, 646
TaqII GACCGA 1 cut(s) 255
TasI AATT 2 cut(s) 199, 289
TatI WGTACW 1 cut(s) 114
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 2 cut(s) 64, 643
TscAI CASTG 2 cut(s) 90, 102
TseFI GTSAC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 2 cut(s) 208, 605
TspRI CASTG 2 cut(s) 90, 102
VpaK11BI GGWCC 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 2 cut(s) 215, 254
Zsp2I ATGCAT 2 cut(s) 217, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.