Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013532

Physical Location & Seq

Genomic Coordinates Forward (+)

24033994 .. 24036148

Gene Structure 2155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1160500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGGTCGACTTCCCCAAGTCTCTCTCGCCGAAGCGAGTCCTGAAGCTCCTCAAAGCCGAGAAGAACCCTCATTCCGCACTCGCCCTGCTCGACTCAGCGACCCGCCACCCGAACTACAACCACTCCCCCGACGTCTTCCACCACATTCTTCGTCGGCTCGTGGACCCGAAGCTCGTCGTTCACGTCGAGCGGGTGGTCGAGCTGATTCGGACCCAGAAATGCAAGTGCCCCGAGGACGTGGCATTGACGGTGATCAAAGCGTATACGAAAAACTCCATGCCCGATAAAGCGTTGGCCGTGTTTCAGCAAATGGAAGAGATTTTTGGGTGTGCGCCCGGCGTAAGGTCGTACAATTCGCTGCTGAATGCGTTCATTGAGTCGAACCAATGGGACCGAGCTGAGAAATTTTTTGCTTATTTCGAAACGGTGGGTTTGGAGCCAAATTTGCAAACTTATAATGTTCTGATCAAGATTTCGTGCAAGAAGAAGCAGTTTGAGAAGGCTAGAGGGTTGCTGAATTGGATGTGGGAAAAGGGTTTGGAGCCTGATGTGTTTAGTTATGGGACTTTGATCAACGGGCTTGCGAAAGGTGGGAACTTGAGTGATGCATTGGAGGTGTTTGATGAAATGTTTGAGAGAGGGGTTGGTCCTGATGTGATGTGTTATAATATTTTGATTGATGGTTTTTTCCGGAAAGGCGATAGTGTGAGTGCCAATGAGATTTGGGAGAGGTTGGTGAAGGACTCAGAGGTTTATCCTAATATTGTTACTTATAACGTTATGATCAATGGTTTGTGTAAATGTGGGAAGTTCAGTGAGAGTTTAGAGATATGGAATCGGATGAAGACAAATGATCGAGGGCCTGACTTGTATACTTGTAGTTCTTTGATTCATGGGTTGTGCAAAGCAGGGAATGTGGATGGGGCTGAGAGAGTTTATAAAGAGATGGTTGATAAAGGGGTTGTTCCGGATGTGGTTGTCTATAATGCAATGCTCAATGGGTTCTGTATAGCAGGGAAGACTAAAGAGTGCTTTGAGCTGTGGGACATGATGGAGATGTGTGGTTGTCGTAATGTTGTGAGTTATAACACATTGATTAGAGGGTTGTTTGAAAACGAGAAGGTTGATGAAGCAATTTCTGTCTGGGGACTAATGTGTGATAAGGCTTGTGTTGCAGATGCCACAACCTATGGAGTATTAATCCATGGACTGTGTAGAAATGGGTATTTAAACAAGGCTTTGCAGATTTTAAAAGTGGCAGAAAACACAGGAGCTGATCTGGATGCCTTTGCGTATTCGTCAATGATTAATGGGTTATGCAAGGAAGGGATACTAGATGAAGCAGCCAGGCTAGTGGGTAAGATGGATAAGTGTGGCTATGAACCGAATTCTCATGTTTGCAATGCATTGATATACGGGTATATCCAAGCTTCCAAACTTGAAGATGCCATTTTGTTTTTTAGAGGAATGTGCACCAAGTTTTGCTCTCCAAATGTTATCTCCTACAATACTCTCATAAATGGTTTATGCAAAGCAGAAAGATTTAGTGACGCATATGTGTTTGTGAGGGAAATGCTGGAAAAAGGATGGAAGCCGGATGTGATCACATATAGCTTGTTAATCGATGGCCTTTGTCAAGGCAAAAAGATTGACATGGCCCTCAACGTGTGGCATCAAGCCCTTGACAAGGGCTTCGAGCCTGATGTAACTATGCATAACATTATAATTCATGGTCTTTGCTCTGCAGGCAAGGCTGAAGATGCTTTGCAGCTTTATTTTCAGATGTGGCGTTGGGACTGCGTTCCAAATCTTGTAACCTACAACACCCTAATGGAGGGTTTTTACAAAATTACAGACTGTGAAAAGGCATCAGAAATTTGGGCCTGCCTTCTAAAAGACGGACTACAACCAGATATCATCACCTATAATGTTACTCTCAAAGGGTTTTGTTCATGCAGTAGAATATCAGATGCCATTAGGTTTTTAGAAAAGGCTTTACGTCTTGGAATTCTTCCAACTTCCATTACGTGGTACATACTTGTTAGGGCAGTGCTAAACAATGGGACCACTTCAAACTCTTCTTGGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

697

Amino Acids

78.44

Weight (kDa)

6.13

Isoelectric Point (pI)

26.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	83 - 124	6.1e-06	PPR repeat family
PPR_2	PF13041	112 - 161	4.7e-10	PPR repeat family
PPR_3	PF13812	114 - 158	5.1e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	132 - 227	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	147 - 196	2.2e-14	PPR repeat family
PPR_3	PF13812	173 - 227	3e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	178 - 210	1.2e-11	PPR repeat
PPR_2	PF13041	182 - 230	1.8e-14	PPR repeat family
PPR	PF01535	185 - 215	7.4e-08	PPR repeat
PPR_3	PF13812	213 - 263	1.9e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	231 - 353	8e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	251 - 282	1.4e-09	PPR repeat
PPR_2	PF13041	253 - 302	2.3e-16	PPR repeat family
PPR	PF01535	256 - 284	7.1e-09	PPR repeat
PPR_3	PF13812	279 - 333	9e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	288 - 336	4e-15	PPR repeat family
PPR_1	PF12854	288 - 317	1.3e-08	PPR repeat
PPR	PF01535	291 - 321	2.9e-08	PPR repeat
PPR_3	PF13812	311 - 366	1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	319 - 352	1.6e-09	PPR repeat
PPR_2	PF13041	323 - 356	1.5e-07	PPR repeat family
PPR	PF01535	326 - 356	4.7e-07	PPR repeat
PPR_1	PF12854	356 - 385	1.6e-06	PPR repeat
PPR_2	PF13041	357 - 406	1.5e-14	PPR repeat family
PPR_1	PF12854	390 - 418	2.2e-07	PPR repeat
PPR_2	PF13041	393 - 441	5.4e-10	PPR repeat family
PPR_1	PF12854	425 - 455	6.4e-08	PPR repeat
PPR_2	PF13041	428 - 465	9.4e-07	PPR repeat family
PPR_3	PF13812	450 - 507	2.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	462 - 511	2.2e-12	PPR repeat family
PPR_1	PF12854	495 - 525	3e-12	PPR repeat
PPR_2	PF13041	497 - 546	3.4e-18	PPR repeat family
PPR	PF01535	500 - 529	2e-06	PPR repeat
PPR_1	PF12854	528 - 558	9.1e-07	PPR repeat
PPR_2	PF13041	540 - 580	3.4e-07	PPR repeat family
PPR_3	PF13812	558 - 612	5.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	563 - 595	3e-08	PPR repeat
PPR_2	PF13041	567 - 616	6.1e-13	PPR repeat family
PPR_3	PF13812	600 - 647	1.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	602 - 650	1.6e-11	PPR repeat family
PPR_1	PF12854	633 - 664	4.5e-06	PPR repeat
PPR_2	PF13041	637 - 683	2.3e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013532)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09060 AT3G09060
fragaria_vesca	FvH4_6g11981
malus_domestica	MD04G1147200.v1.1 MD12G1160500.v1.1
prunus_persica	Prupe.6G271000_v2.0.a1
pyrus_communis	pycom12g15230
rosa_chinensis	RchiOBHm_Chr3g0463821
rosa_laevigata	RLG00000024754
rosa_multiflora	Rmu_sc0004796.1_g000010
rosa_roxburghii	Rroxscaffold_6G00416270
rosa_rugosa	Rorug03G0068000 Rorug03G0068100
rosa_samantha	Rh3AG125000 Rh3BG129300 Rh3CG131900 Rh3DG130400
rosa_wichuraiana	Rw3G010560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	6 cut(s)	456, 666, 774, 939, 1086, 1724
AatII	GACGTC	1 cut(s)	135
AccB7I	CCANNNNNTGG	1 cut(s)	2028
AccBSI	CCGCTC	1 cut(s)	190
AccI	GTMKAC	3 cut(s)	6, 263, 872
AccIII	TCCGGA	2 cut(s)	690, 967
AciI	CCGC	3 cut(s)	75, 103, 190
AclI	AACGTT	1 cut(s)	777
AcoI	YGGCCR	1 cut(s)	294
AcsI	RAATTY	5 cut(s)	404, 442, 1387, 1875, 2007
AcuI	CTGAAG	2 cut(s)	62, 1776
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	2 cut(s)	350, 2033
AfiI	CCNNNNNNNGG	4 cut(s)	342, 1687, 1834, 2028
AflIII	ACRYGT	1 cut(s)	1665
AgsI	TTSAA	3 cut(s)	1112, 1442, 2073
AjiI	CACGTC	2 cut(s)	184, 238
AjnI	CCWGG	1 cut(s)	1346
AjuI	GAANNNNNNNTTGG	2 cut(s)	306, 338
AloI	GAACNNNNNNTCC	2 cut(s)	56, 88
AluBI	AGCT	9 cut(s)	46, 172, 202, 398, 1039, 1274, 1430, 1614, 1771
AluI	AGCT	9 cut(s)	46, 172, 202, 398, 1039, 1274, 1430, 1614, 1771
Alw21I	GWGCWC	1 cut(s)	1475
Alw26I	GTCTC	1 cut(s)	24
Alw44I	GTGCAC	1 cut(s)	1471
AlwNI	CAGNNNCTG	1 cut(s)	1274
Ama87I	CYCGRG	1 cut(s)	230
Aor13HI	TCCGGA	2 cut(s)	690, 967
AoxI	GGCC	5 cut(s)	294, 860, 1627, 1656, 1881
ApaLI	GTGCAC	1 cut(s)	1471
ApeKI	GCWGC	3 cut(s)	358, 1343, 1768
ApoI	RAATTY	5 cut(s)	404, 442, 1387, 1875, 2007
ArsI	GACNNNNNNTTYG	2 cut(s)	1676, 1708
AseI	ATTAAT	2 cut(s)	1199, 1308
Asp700I	GAANNNNTTC	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	334
AspS9I	GGNCC	8 cut(s)	163, 210, 391, 647, 860, 1657, 1881, 2064
AsuC2I	CCSGG	1 cut(s)	336
AsuHPI	GGTGA	3 cut(s)	262, 748, 1912
AsuII	TTCGAA	1 cut(s)	420
AvaI	CYCGRG	1 cut(s)	230
AvaII	GGWCC	5 cut(s)	163, 210, 391, 647, 2064
BaeGI	GKGCMC	2 cut(s)	230, 1475
BauI	CACGAG	1 cut(s)	158
BbsI	GAAGAC	3 cut(s)	127, 851, 1025
Bbv12I	GWGCWC	1 cut(s)	1475
BbvI	GCAGC	3 cut(s)	345, 1355, 1780
BccI	CCATC	7 cut(s)	674, 914, 940, 1045, 1357, 1581, 1619
BceAI	ACGGC	1 cut(s)	281
BciT130I	CCWGG	1 cut(s)	1348
BciVI	GTATCC	1 cut(s)	1323
BclI	TGATCA	5 cut(s)	252, 465, 570, 783, 1602
BcnI	CCSGG	1 cut(s)	336
BcoDI	GTCTC	1 cut(s)	24
BfaI	CTAG	3 cut(s)	504, 1334, 1352
BfmI	CTRYAG	1 cut(s)	1743
BfuI	GTATCC	1 cut(s)	1323
BisI	GCNGC	3 cut(s)	359, 1344, 1769
BlsI	GCNGC	3 cut(s)	360, 1345, 1770
Bme1390I	CCNGG	2 cut(s)	336, 1348
Bme18I	GGWCC	5 cut(s)	163, 210, 391, 647, 2064
BmeT110I	CYCGRG	1 cut(s)	230
BmgBI	CACGTC	2 cut(s)	184, 238
BmgT120I	GGNCC	8 cut(s)	163, 210, 391, 647, 860, 1657, 1881, 2064
BmiI	GGNNCC	6 cut(s)	165, 212, 392, 438, 543, 2065
BmrFI	CCNGG	2 cut(s)	336, 1348
BmsI	GCATC	8 cut(s)	595, 1168, 1273, 1435, 1681, 1750, 1877, 1960
BpiI	GAAGAC	3 cut(s)	127, 851, 1025
Bpu14I	TTCGAA	1 cut(s)	420
BpuEI	CTTGAG	1 cut(s)	619
BpuMI	CCSGG	1 cut(s)	336
Bsa29I	ATCGAT	1 cut(s)	1623
BsaAI	YACGTR	1 cut(s)	2028
BsaHI	GRCGYC	1 cut(s)	132
BsaJI	CCNNGG	2 cut(s)	231, 1204
BsaWI	WCCGGW	2 cut(s)	690, 967
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bsc4I	CCNNNNNNNGG	4 cut(s)	342, 1687, 1834, 2028
Bse3DI	GCAATG	2 cut(s)	996, 1408
BseAI	TCCGGA	2 cut(s)	690, 967
BseBI	CCWGG	1 cut(s)	1348
BseCI	ATCGAT	1 cut(s)	1623
BseDI	CCNNGG	2 cut(s)	231, 1204
BseGI	GGATG	7 cut(s)	528, 846, 925, 976, 1288, 1592, 1603
BseLI	CCNNNNNNNGG	4 cut(s)	342, 1687, 1834, 2028
BseMI	GCAATG	2 cut(s)	996, 1408
BseMII	CTCAG	4 cut(s)	108, 390, 759, 918
BseRI	GAGGAG	1 cut(s)	38
BseSI	GKGCMC	2 cut(s)	230, 1475
BseXI	GCAGC	3 cut(s)	345, 1355, 1780
BshFI	GGCC	5 cut(s)	296, 862, 1629, 1658, 1883
BshVI	ATCGAT	1 cut(s)	1623
BsiHKAI	GWGCWC	1 cut(s)	1475
BsiHKCI	CYCGRG	1 cut(s)	230
BsiSI	CCGG	4 cut(s)	336, 691, 968, 1595
BslFI	GGGAC	6 cut(s)	404, 577, 1058, 1161, 1808, 2077
BslI	CCNNNNNNNGG	4 cut(s)	342, 1687, 1834, 2028
BsmAI	GTCTC	1 cut(s)	24
BsmFI	GGGAC	6 cut(s)	404, 577, 1058, 1161, 1808, 2077
BsmI	GAATGC	1 cut(s)	370
BsnI	GGCC	5 cut(s)	296, 862, 1629, 1658, 1883
BsoBI	CYCGRG	1 cut(s)	230
Bsp119I	TTCGAA	1 cut(s)	420
Bsp1286I	GDGCHC	2 cut(s)	230, 1475
Bsp13I	TCCGGA	2 cut(s)	690, 967
Bsp143I	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
Bsp19I	CCATGG	1 cut(s)	1204
BspACI	CCGC	3 cut(s)	75, 103, 190
BspANI	GGCC	5 cut(s)	296, 862, 1629, 1658, 1883
BspCNI	CTCAG	4 cut(s)	107, 391, 758, 919
BspDI	ATCGAT	1 cut(s)	1623
BspEI	TCCGGA	2 cut(s)	690, 967
BspLI	GGNNCC	6 cut(s)	165, 212, 392, 438, 543, 2065
BspMAI	CTGCAG	1 cut(s)	1747
BspT104I	TTCGAA	1 cut(s)	420
BsrBI	CCGCTC	1 cut(s)	190
BsrDI	GCAATG	2 cut(s)	996, 1408
BssECI	CCNNGG	2 cut(s)	231, 1204
BssMI	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
BssNAI	GTATAC	2 cut(s)	264, 873
BssNI	GRCGYC	1 cut(s)	132
BssSI	CACGAG	1 cut(s)	158
BssT1I	CCWWGG	1 cut(s)	1204
Bst1107I	GTATAC	2 cut(s)	264, 873
Bst2BI	CACGAG	1 cut(s)	158
Bst2UI	CCWGG	1 cut(s)	1348
Bst4CI	ACNGT	4 cut(s)	250, 427, 1212, 1859
Bst6I	CTCTTC	2 cut(s)	309, 2083
BstACI	GRCGYC	1 cut(s)	132
BstBAI	YACGTR	1 cut(s)	2028
BstBI	TTCGAA	1 cut(s)	420
BstC8I	GCNNGC	3 cut(s)	582, 1747, 1885
BstDEI	CTNAG	4 cut(s)	94, 399, 745, 927
BstDSI	CCRYGG	1 cut(s)	1204
BstENI	CCTNNNNNAGG	2 cut(s)	1685, 1832
BstF5I	GGATG	7 cut(s)	528, 846, 925, 976, 1288, 1592, 1603
BstHHI	GCGC	1 cut(s)	334
BstKTI	GATC	7 cut(s)	255, 468, 573, 786, 856, 1279, 1605
BstMAI	GTCTC	1 cut(s)	24
BstMBI	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
BstMWI	GCNNNNNNNGC	5 cut(s)	445, 1172, 1746, 1751, 1760
BstNI	CCWGG	1 cut(s)	1348
BstSCI	CCNGG	2 cut(s)	334, 1346
BstSFI	CTRYAG	1 cut(s)	1743
BstSLI	GKGCMC	2 cut(s)	230, 1475
BstV1I	GCAGC	3 cut(s)	345, 1355, 1780
BstV2I	GAAGAC	3 cut(s)	127, 851, 1025
BstXI	CCANNNNNNTGG	1 cut(s)	1354
BstZ17I	GTATAC	2 cut(s)	264, 873
Bsu15I	ATCGAT	1 cut(s)	1623
BsuI	GTATCC	1 cut(s)	1323
BsuRI	GGCC	5 cut(s)	296, 862, 1629, 1658, 1883
BsuTUI	ATCGAT	1 cut(s)	1623
BtgI	CCRYGG	1 cut(s)	1204
BtrI	CACGTC	2 cut(s)	184, 238
BtsCI	GGATG	7 cut(s)	528, 846, 925, 976, 1288, 1592, 1603
BtsI	GCAGTG	1 cut(s)	2055
BtsIMutI	CAGTG	2 cut(s)	820, 2055
Cac8I	GCNNGC	3 cut(s)	582, 1747, 1885
CaiI	CAGNNNCTG	1 cut(s)	1274
CfoI	GCGC	1 cut(s)	334
Cfr13I	GGNCC	8 cut(s)	163, 210, 391, 647, 860, 1657, 1881, 2064
ClaI	ATCGAT	1 cut(s)	1623
CseI	GACGC	1 cut(s)	1559
Csp6I	GTAC	2 cut(s)	349, 2032
CviAII	CATG	8 cut(s)	277, 893, 1048, 1205, 1394, 1654, 1730, 1953
CviQI	GTAC	2 cut(s)	349, 2032
DdeI	CTNAG	4 cut(s)	94, 399, 745, 927
DpnI	GATC	7 cut(s)	254, 467, 572, 785, 855, 1278, 1604
DpnII	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
DraI	TTTAAA	2 cut(s)	1230, 1251
EaeI	YGGCCR	1 cut(s)	294
Eam1104I	CTCTTC	2 cut(s)	309, 2083
EarI	CTCTTC	2 cut(s)	309, 2083
Eco130I	CCWWGG	1 cut(s)	1204
Eco32I	GATATC	1 cut(s)	1915
Eco47I	GGWCC	5 cut(s)	163, 210, 391, 647, 2064
Eco57I	CTGAAG	2 cut(s)	62, 1776
Eco88I	CYCGRG	1 cut(s)	230
EcoNI	CCTNNNNNAGG	2 cut(s)	1685, 1832
EcoO109I	RGGNCCY	1 cut(s)	860
EcoRI	GAATTC	2 cut(s)	1387, 2007
EcoRII	CCWGG	1 cut(s)	1346
EcoRV	GATATC	1 cut(s)	1915
EcoT14I	CCWWGG	1 cut(s)	1204
EcoT22I	ATGCAT	3 cut(s)	610, 1408, 1716
ErhI	CCWWGG	1 cut(s)	1204
FaeI	CATG	8 cut(s)	280, 896, 1051, 1208, 1397, 1657, 1733, 1956
FaqI	GGGAC	6 cut(s)	404, 577, 1058, 1161, 1808, 2077
FatI	CATG	8 cut(s)	276, 892, 1047, 1204, 1393, 1653, 1729, 1952
FauI	CCCGC	2 cut(s)	110, 183
FauNDI	CATATG	1 cut(s)	1555
FbaI	TGATCA	5 cut(s)	252, 465, 570, 783, 1602
FblI	GTMKAC	3 cut(s)	6, 263, 872
Fnu4HI	GCNGC	3 cut(s)	359, 1344, 1769
FokI	GGATG	7 cut(s)	535, 853, 932, 983, 1295, 1599, 1610
Fsp4HI	GCNGC	3 cut(s)	359, 1344, 1769
FspBI	CTAG	3 cut(s)	504, 1334, 1352
GlaI	GCGC	1 cut(s)	333
GluI	GCNGC	3 cut(s)	359, 1344, 1769
HaeIII	GGCC	5 cut(s)	296, 862, 1629, 1658, 1883
HapII	CCGG	4 cut(s)	336, 691, 968, 1595
HgaI	GACGC	1 cut(s)	1559
HhaI	GCGC	1 cut(s)	334
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	8 cut(s)	280, 896, 1051, 1208, 1397, 1657, 1733, 1956
Hin6I	GCGC	1 cut(s)	332
HinP1I	GCGC	1 cut(s)	332
HincII	GTYRAC	1 cut(s)	7
HindII	GTYRAC	1 cut(s)	7
HindIII	AAGCTT	1 cut(s)	1428
HinfI	GANTC	7 cut(s)	36, 92, 205, 377, 743, 835, 889
HpaII	CCGG	4 cut(s)	336, 691, 968, 1595
HphI	GGTGA	3 cut(s)	262, 748, 1912
Hpy166II	GTNNAC	6 cut(s)	7, 163, 181, 264, 873, 1473
Hpy188I	TCNGA	7 cut(s)	210, 465, 748, 840, 1782, 1873, 1969
Hpy188III	TCNNGA	6 cut(s)	40, 469, 650, 691, 968, 1280
Hpy8I	GTNNAC	6 cut(s)	7, 163, 181, 264, 873, 1473
Hpy99I	CGWCG	4 cut(s)	134, 156, 179, 188
HpyAV	CCTTC	5 cut(s)	493, 733, 1114, 1319, 1898
HpyCH4III	ACNGT	4 cut(s)	250, 427, 1212, 1859
HpyCH4IV	ACGT	7 cut(s)	132, 183, 237, 777, 1665, 1999, 2027
HpyF10VI	GCNNNNNNNGC	5 cut(s)	445, 1172, 1746, 1751, 1760
HpyF3I	CTNAG	4 cut(s)	94, 399, 745, 927
HpySE526I	ACGT	7 cut(s)	132, 183, 237, 777, 1665, 1999, 2027
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	8 cut(s)	280, 896, 1051, 1208, 1397, 1657, 1733, 1956
HspAI	GCGC	1 cut(s)	332
Kpn2I	TCCGGA	2 cut(s)	690, 967
Ksp22I	TGATCA	5 cut(s)	252, 465, 570, 783, 1602
Kzo9I	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
LmnI	GCTCC	4 cut(s)	51, 436, 541, 1271
Lsp1109I	GCAGC	3 cut(s)	345, 1355, 1780
LweI	GCATC	8 cut(s)	595, 1168, 1273, 1435, 1681, 1750, 1877, 1960
MaeI	CTAG	3 cut(s)	504, 1334, 1352
MaeII	ACGT	7 cut(s)	132, 183, 237, 777, 1665, 1999, 2027
MaeIII	GTNAC	5 cut(s)	766, 1547, 1705, 1813, 1930
MalI	GATC	7 cut(s)	254, 467, 572, 785, 855, 1278, 1604
MbiI	CCGCTC	1 cut(s)	190
MboI	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
MhlI	GDGCHC	2 cut(s)	230, 1475
MlyI	GAGTC	4 cut(s)	45, 86, 386, 737
MmeI	TCCRAC	1 cut(s)	2039
Mph1103I	ATGCAT	3 cut(s)	610, 1408, 1716
MroI	TCCGGA	2 cut(s)	690, 967
MroXI	GAANNNNTTC	1 cut(s)	368
MseI	TTAA	5 cut(s)	1199, 1229, 1250, 1308, 1619
MslI	CAYNNNNRTG	1 cut(s)	1829
MspI	CCGG	4 cut(s)	336, 691, 968, 1595
MspR9I	CCNGG	2 cut(s)	336, 1348
Mva1269I	GAATGC	1 cut(s)	370
MvaI	CCWGG	1 cut(s)	1348
MwoI	GCNNNNNNNGC	5 cut(s)	445, 1172, 1746, 1751, 1760
NciI	CCSGG	1 cut(s)	336
NcoI	CCATGG	1 cut(s)	1204
NdeI	CATATG	1 cut(s)	1555
NdeII	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
NlaIII	CATG	8 cut(s)	280, 896, 1051, 1208, 1397, 1657, 1733, 1956
NlaIV	GGNNCC	6 cut(s)	165, 212, 392, 438, 543, 2065
NmeAIII	GCCGAG	1 cut(s)	82
NmuCI	GTSAC	1 cut(s)	1547
NsiI	ATGCAT	3 cut(s)	610, 1408, 1716
NspV	TTCGAA	1 cut(s)	420
PcsI	WCGNNNNNNNCGW	3 cut(s)	87, 180, 183
PctI	GAATGC	1 cut(s)	370
PdmI	GAANNNNTTC	1 cut(s)	368
PfeI	GAWTC	3 cut(s)	205, 835, 889
PflMI	CCANNNNNTGG	1 cut(s)	2028
PkrI	GCNGC	3 cut(s)	360, 1345, 1770
PleI	GAGTC	4 cut(s)	44, 86, 385, 737
PpsI	GAGTC	4 cut(s)	44, 86, 385, 737
Ppu21I	YACGTR	1 cut(s)	2028
PshBI	ATTAAT	2 cut(s)	1199, 1308
PsiI	TTATAA	6 cut(s)	456, 666, 774, 939, 1086, 1724
Psp1406I	AACGTT	1 cut(s)	777
Psp6I	CCWGG	1 cut(s)	1346
PspGI	CCWGG	1 cut(s)	1346
PspN4I	GGNNCC	6 cut(s)	165, 212, 392, 438, 543, 2065
PspPI	GGNCC	8 cut(s)	163, 210, 391, 647, 860, 1657, 1881, 2064
PstI	CTGCAG	1 cut(s)	1747
PstNI	CAGNNNCTG	1 cut(s)	1274
RsaI	GTAC	2 cut(s)	350, 2033
RsaNI	GTAC	2 cut(s)	349, 2032
RseI	CAYNNNNRTG	1 cut(s)	1829
SalI	GTCGAC	1 cut(s)	5
SaqAI	TTAA	5 cut(s)	1199, 1229, 1250, 1308, 1619
SatI	GCNGC	3 cut(s)	359, 1344, 1769
Sau3AI	GATC	7 cut(s)	252, 465, 570, 783, 853, 1276, 1602
Sau96I	GGNCC	8 cut(s)	163, 210, 391, 647, 860, 1657, 1881, 2064
SchI	GAGTC	4 cut(s)	45, 86, 386, 737
ScrFI	CCNGG	2 cut(s)	336, 1348
SduI	GDGCHC	2 cut(s)	230, 1475
SfaNI	GCATC	8 cut(s)	595, 1168, 1273, 1435, 1681, 1750, 1877, 1960
SfcI	CTRYAG	1 cut(s)	1743
SfuI	TTCGAA	1 cut(s)	420
SinI	GGWCC	5 cut(s)	163, 210, 391, 647, 2064
SmiMI	CAYNNNNRTG	1 cut(s)	1829
SmlI	CTYRAG	1 cut(s)	598
SmoI	CTYRAG	1 cut(s)	598
SsiI	CCGC	3 cut(s)	75, 103, 190
SspI	AATATT	2 cut(s)	670, 763
SspMI	CTAG	3 cut(s)	504, 1334, 1352
StyD4I	CCNGG	2 cut(s)	334, 1346
StyI	CCWWGG	1 cut(s)	1204
TaaI	ACNGT	4 cut(s)	250, 427, 1212, 1859
TaiI	ACGT	7 cut(s)	135, 186, 240, 780, 1668, 2002, 2030
TaqI	TCGA	9 cut(s)	6, 90, 186, 198, 380, 420, 856, 1623, 1695
TaqII	GACCGA	1 cut(s)	408
TfiI	GAWTC	3 cut(s)	205, 835, 889
Tru1I	TTAA	5 cut(s)	1199, 1229, 1250, 1308, 1619
Tru9I	TTAA	5 cut(s)	1199, 1229, 1250, 1308, 1619
TscAI	CASTG	2 cut(s)	820, 2055
TseFI	GTSAC	1 cut(s)	1547
TseI	GCWGC	3 cut(s)	358, 1343, 1768
Tsp45I	GTSAC	1 cut(s)	1547
TspDTI	ATGAA	9 cut(s)	361, 639, 857, 881, 1143, 1353, 1395, 1718, 1941
TspGWI	ACGGA	1 cut(s)	1914
TspRI	CASTG	2 cut(s)	820, 2055
Van91I	CCANNNNNTGG	1 cut(s)	2028
VneI	GTGCAC	1 cut(s)	1471
VpaK11BI	GGWCC	5 cut(s)	163, 210, 391, 647, 2064
VspI	ATTAAT	2 cut(s)	1199, 1308
XagI	CCTNNNNNAGG	2 cut(s)	1685, 1832
XapI	RAATTY	5 cut(s)	404, 442, 1387, 1875, 2007
XmiI	GTMKAC	3 cut(s)	6, 263, 872
XmnI	GAANNNNTTC	1 cut(s)	368
XspI	CTAG	3 cut(s)	504, 1334, 1352
ZraI	GACGTC	1 cut(s)	133
Zsp2I	ATGCAT	3 cut(s)	610, 1408, 1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.