MD04G1147400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014486
Physical Location & Seq
Reverse (-)
23560785 .. 23562255
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1147400.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGATAATGTAACGCCAGATGAGAGGCTCACAAGCACTTTCAACAGTTCTAACTGTCGACATACAAATCTCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCCTCTCCTCTCTCTGCCCTTTATCCAAACTCAAAACCAGGACTGAAACCGGAGAAGATGATTGGCCTCAAGCTCATTCAAACCTCTTTCACCACCACCCAACATACCATTTTATACACAGGAAGATCGTCCAGCGCCAAGACGTCCATCTTGCGCTGCAGCAAGTCCAATGATTCAGACTCCGAGTCGGAAGCTTCCCCGCCCGAAGGCGACACTCGCAAGCAAGAGCTGCTGGTGAGGATAGCAATGCTTCAAGCTCAGAAAGTCCGTCTTGCCGATTACTTGGACGAAAGGTCAGAATATCTTACCAAATTTGGAGAGGAGGTAACCGCCGAGTTTGACAAGATTGGAGAAGATGCCCTCAAAGACCTGGACGCAGCCAGCGACAGGATATTGGAGAATATAAACAGCCGCATGCAAGCCTTTGAGGAATCTGCGGGAGTAAACATAGTGGAGATGGAGAAAAACGAAAACGAGTTAGAAGCATTCGAAGGTCAGATTGAAAAGGAGCGAAATGAAGGGTTATTTTTCAAGAGTCTAACGCAGGGGAAACCGAAAGAAAAAGTGAACGCTGCCGAGGAAATAAATAAGATCAAGGAGCTTACCAAAAACACTGCAGGATCAGAAATCAGGAGGAATATTTACCTTGCACTAATTGGCCTGCTACTCATACAACTCGTCGACTCTTTCATCTCTCCAACACCTGATTGGAGAAAAGTTGCACTTCTCGGGGCGATTTTTGTGGGTTTGGTTTCTCAGGTCATCTATGAGCAGAAAATGTTATCAGAAACAGAAAGTACAGAAGAGGGGAAGACAGAGGAAGAAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.69

Weight (kDa)

4.97

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09050
fragaria_vesca FvH4_6g11980
malus_domestica MD04G1147400.v1.1
pyrus_communis pycom04g13300 pycom12g15240
rosa_chinensis RchiOBHm_Chr3g0463811
rosa_laevigata RLG00000024755
rosa_multiflora Rmu_sc0004796.1_g000012
rosa_roxburghii Rroxscaffold_6G00416280
rosa_rugosa Rorug03G0067800 Rorug03G0067900 Rorug03G0067900
rosa_samantha Rh3AG124900 Rh3BG129400 Rh3CG131800 Rh3DG130300
rosa_wichuraiana Rw3G010570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 245
AccI GTMKAC 2 cut(s) 58, 780
AciI CCGC 4 cut(s) 299, 429, 511, 536
AclWI GGATC 1 cut(s) 727
AcsI RAATTY 1 cut(s) 410
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 1 cut(s) 898
AfiI CCNNNNNNNGG 2 cut(s) 305, 486
AgsI TTSAA 5 cut(s) 43, 179, 353, 602, 631
AhdI GACNNNNNGTC 2 cut(s) 283, 391
AjnI CCWGG 2 cut(s) 136, 468
AluBI AGCT 5 cut(s) 172, 293, 328, 356, 700
AluI AGCT 5 cut(s) 172, 293, 328, 356, 700
AlwI GGATC 1 cut(s) 727
Ama87I CYCGRG 1 cut(s) 827
AoxI GGCC 2 cut(s) 163, 757
ApeKI GCWGC 5 cut(s) 255, 258, 328, 476, 671
ApoI RAATTY 1 cut(s) 410
AspLEI GCGC 2 cut(s) 236, 255
AsuHPI GGTGA 2 cut(s) 181, 346
AsuII TTCGAA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 917
BbvI GCAGC 5 cut(s) 242, 270, 315, 488, 658
BccI CCATC 2 cut(s) 254, 550
BciT130I CCWGG 2 cut(s) 138, 470
BfmI CTRYAG 2 cut(s) 256, 714
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 6 cut(s) 256, 259, 329, 477, 511, 672
BlsI GCNGC 6 cut(s) 257, 260, 330, 478, 512, 673
Bme1390I CCNGG 2 cut(s) 138, 470
BmeRI GACNNNNNGTC 2 cut(s) 283, 391
BmeT110I CYCGRG 1 cut(s) 827
BmrFI CCNGG 2 cut(s) 138, 470
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 917
Bpu14I TTCGAA 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 486
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 138, 470
BseDI CCNNGG 1 cut(s) 675
BseLI CCNNNNNNNGG 2 cut(s) 305, 486
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 2 cut(s) 371, 869
BseRI GAGGAG 4 cut(s) 68, 91, 96, 434
BseXI GCAGC 5 cut(s) 242, 270, 315, 488, 658
BshFI GGCC 2 cut(s) 165, 759
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 305, 486
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 2 cut(s) 165, 759
BsoBI CYCGRG 1 cut(s) 827
Bsp119I TTCGAA 1 cut(s) 588
Bsp143I GATC 3 cut(s) 224, 690, 719
BspACI CCGC 4 cut(s) 299, 429, 511, 536
BspANI GGCC 2 cut(s) 165, 759
BspCNI CTCAG 2 cut(s) 370, 868
BspMAI CTGCAG 2 cut(s) 260, 718
BspPI GGATC 1 cut(s) 727
BspT104I TTCGAA 1 cut(s) 588
BsrDI GCAATG 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 3 cut(s) 224, 690, 719
BssNI GRCGYC 1 cut(s) 242
Bst2UI CCWGG 2 cut(s) 138, 470
Bst4CI ACNGT 2 cut(s) 47, 56
Bst6I CTCTTC 1 cut(s) 897
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 5 cut(s) 320, 481, 515, 519, 761
BstDEI CTNAG 2 cut(s) 357, 855
BstEII GGTNACC 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 255
BstKTI GATC 3 cut(s) 227, 693, 722
BstMBI GATC 3 cut(s) 224, 690, 719
BstMWI GCNNNNNNNGC 2 cut(s) 315, 328
BstNI CCWGG 2 cut(s) 138, 470
BstNSI RCATGY 1 cut(s) 517
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 136, 468
BstSFI CTRYAG 2 cut(s) 256, 714
BstV1I GCAGC 5 cut(s) 242, 270, 315, 488, 658
BstV2I GAAGAC 1 cut(s) 917
BsuRI GGCC 2 cut(s) 165, 759
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 1 cut(s) 711
Cac8I GCNNGC 5 cut(s) 320, 481, 515, 519, 761
CfoI GCGC 2 cut(s) 236, 255
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 897
CviAII CATG 1 cut(s) 514
CviQI GTAC 1 cut(s) 897
DdeI CTNAG 2 cut(s) 357, 855
DpnI GATC 3 cut(s) 226, 692, 721
DpnII GATC 3 cut(s) 224, 690, 719
DriI GACNNNNNGTC 2 cut(s) 283, 391
Eam1104I CTCTTC 1 cut(s) 897
Eam1105I GACNNNNNGTC 2 cut(s) 283, 391
EarI CTCTTC 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 136, 468
FaeI CATG 1 cut(s) 517
FaiI YATR 8 cut(s) 63, 204, 214, 503, 515, 548, 770, 867
FalI AAGNNNNNCTT 4 cut(s) 354, 386, 807, 839
FatI CATG 1 cut(s) 513
FauI CCCGC 2 cut(s) 306, 529
FblI GTMKAC 2 cut(s) 58, 780
Fnu4HI GCNGC 6 cut(s) 256, 259, 329, 477, 511, 672
Fsp4HI GCNGC 6 cut(s) 256, 259, 329, 477, 511, 672
GlaI GCGC 2 cut(s) 235, 254
GluI GCNGC 6 cut(s) 256, 259, 329, 477, 511, 672
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 165, 759
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 236, 255
Hin1I GRCGYC 1 cut(s) 242
Hin1II CATG 1 cut(s) 517
Hin6I GCGC 2 cut(s) 234, 253
HinP1I GCGC 2 cut(s) 234, 253
HincII GTYRAC 2 cut(s) 59, 781
HindII GTYRAC 2 cut(s) 59, 781
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 6 cut(s) 272, 278, 284, 530, 634, 782
HpaII CCGG 1 cut(s) 149
HphI GGTGA 2 cut(s) 181, 346
Hpy166II GTNNAC 4 cut(s) 59, 544, 667, 781
Hpy188I TCNGA 8 cut(s) 277, 283, 289, 360, 397, 597, 724, 886
Hpy188III TCNNGA 2 cut(s) 631, 730
Hpy8I GTNNAC 4 cut(s) 59, 544, 667, 781
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 4 cut(s) 299, 584, 611, 921
HpyCH4III ACNGT 2 cut(s) 47, 56
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 5 cut(s) 258, 517, 716, 749, 821
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 328
HpyF3I CTNAG 2 cut(s) 357, 855
HpySE526I ACGT 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 242
Hsp92II CATG 1 cut(s) 517
HspAI GCGC 2 cut(s) 234, 253
Kzo9I GATC 3 cut(s) 224, 690, 719
LmnI GCTCC 2 cut(s) 607, 697
Lsp1109I GCAGC 5 cut(s) 242, 270, 315, 488, 658
LweI GCATC 1 cut(s) 445
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 2 cut(s) 10, 424
MalI GATC 3 cut(s) 226, 692, 721
MboI GATC 3 cut(s) 224, 690, 719
MboII GAAGA 6 cut(s) 166, 234, 464, 914, 922, 932
MluCI AATT 2 cut(s) 410, 753
MlyI GAGTC 4 cut(s) 272, 293, 643, 776
MmeI TCCRAC 2 cut(s) 267, 821
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 138, 470
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 2 cut(s) 138, 470
MwoI GCNNNNNNNGC 2 cut(s) 315, 328
NdeII GATC 3 cut(s) 224, 690, 719
NlaIII CATG 1 cut(s) 517
NmeAIII GCCGAG 2 cut(s) 457, 700
NspI RCATGY 1 cut(s) 517
NspV TTCGAA 1 cut(s) 588
PaeI GCATGC 1 cut(s) 517
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PctI GAATGC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 272, 530
PkrI GCNGC 6 cut(s) 257, 260, 330, 478, 512, 673
PleI GAGTC 4 cut(s) 272, 292, 642, 776
PpsI GAGTC 4 cut(s) 272, 292, 642, 776
Psp6I CCWGG 2 cut(s) 136, 468
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 136, 468
PstI CTGCAG 2 cut(s) 260, 718
RsaI GTAC 1 cut(s) 898
RsaNI GTAC 1 cut(s) 897
SalI GTCGAC 2 cut(s) 57, 779
SatI GCNGC 6 cut(s) 256, 259, 329, 477, 511, 672
Sau3AI GATC 3 cut(s) 224, 690, 719
SchI GAGTC 4 cut(s) 272, 293, 643, 776
ScrFI CCNGG 2 cut(s) 138, 470
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 2 cut(s) 256, 714
SfuI TTCGAA 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SphI GCATGC 1 cut(s) 517
Sse9I AATT 2 cut(s) 410, 753
SsiI CCGC 4 cut(s) 299, 429, 511, 536
SspI AATATT 1 cut(s) 739
StyD4I CCNGG 2 cut(s) 136, 468
TaaI ACNGT 2 cut(s) 47, 56
TaiI ACGT 1 cut(s) 245
TaqI TCGA 3 cut(s) 58, 588, 780
TasI AATT 2 cut(s) 410, 753
TatI WGTACW 1 cut(s) 896
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 2 cut(s) 272, 530
TscAI CASTG 1 cut(s) 718
TseI GCWGC 5 cut(s) 255, 258, 328, 476, 671
TspDTI ATGAA 2 cut(s) 630, 778
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 718
XapI RAATTY 1 cut(s) 410
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 804
XmiI GTMKAC 2 cut(s) 58, 780
ZraI GACGTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.