pycom04g13300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014486
Physical Location & Seq
Reverse (-)
16264560 .. 16265442
883 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13300.1

Sequence Viewer

Length: 777 bp
ATGATTGGCCTCAAGCTCATTCAAACCTCTTTTACCACCACCCAACATACCATTTTATACTCAGGAAGATCGTCCAGCGCCAAGACTTCCATCTTGCGCTGCAGCAAGTCCAATGATTCAGACTCCGAGTCGGAAGCTTCCCCGCCTGAAGGCGACACTCGCAAGCAAGAGCTGCTGGTGAGGATAGCAATGCTTCAAGCTCAGAAAGTCCGTCTTGCCGATTACTTGGACGAAAGGTCAGAATATCTTACCAAATTTGGAGAGGAGGCAACCGCCGAGTTTGACAAGATTGGAGAAGATGCCCTCAAAGACCTGGACGCAGCCAGCGACAGGATATTGGAGAATATAAACAGCCGCATGCAAGCCTTTGAAGAATCTGCAGGAGTAAACATAGTGGAGATGGAGAAAAACGAAAACGACTTAGAAGCATTCGAAGGTCAGATTGAAAAGGAGCGAAATGAAGGGTTATTTTTCAAGAGTCTAACGCAGGGGAAACCGAAAGAAAAAGTGAACGCTGCCGAGGAAATAAATAAGATCAAGGAGCTTACCAAAAACAGTGCAGGATCAGAAATCAGGAGGAATATTTACCTTGCACTAATTGGCCTGCTACTCATACAACTCGTCGACTCTTTCATCTCTCCAACACCTGATTGGAGAAAAGTTGCACTTCTCGGGGCGATTTTTGTGGGTTTGGTTTCTCAGGTCATCTATGAGCAGAAAATGTTATCAGAAACAGAAAGTACAGAAGAGGGCAAGACAGAGGAAGAAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.98

Weight (kDa)

4.82

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09050
fragaria_vesca FvH4_6g11980
malus_domestica MD04G1147400.v1.1
pyrus_communis pycom04g13300 pycom12g15240
rosa_chinensis RchiOBHm_Chr3g0463811
rosa_laevigata RLG00000024755
rosa_multiflora Rmu_sc0004796.1_g000012
rosa_roxburghii Rroxscaffold_6G00416280
rosa_rugosa Rorug03G0067800 Rorug03G0067900 Rorug03G0067900
rosa_samantha Rh3AG124900 Rh3BG129400 Rh3CG131800 Rh3DG130300
rosa_wichuraiana Rw3G010570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 624
AciI CCGC 3 cut(s) 143, 273, 355
AclWI GGATC 1 cut(s) 571
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 742
AfiI CCNNNNNNNGG 2 cut(s) 149, 330
AgsI TTSAA 5 cut(s) 23, 197, 371, 446, 475
AhdI GACNNNNNGTC 2 cut(s) 127, 235
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 5 cut(s) 16, 137, 172, 200, 544
AluI AGCT 5 cut(s) 16, 137, 172, 200, 544
AlwI GGATC 1 cut(s) 571
Ama87I CYCGRG 1 cut(s) 671
AoxI GGCC 2 cut(s) 7, 601
ApeKI GCWGC 5 cut(s) 99, 102, 172, 320, 515
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 2 cut(s) 80, 99
AsuHPI GGTGA 1 cut(s) 190
AsuII TTCGAA 1 cut(s) 432
AvaI CYCGRG 1 cut(s) 671
BbvI GCAGC 5 cut(s) 86, 114, 159, 332, 502
BccI CCATC 2 cut(s) 98, 394
BciT130I CCWGG 1 cut(s) 314
BfmI CTRYAG 2 cut(s) 100, 378
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 6 cut(s) 100, 103, 173, 321, 355, 516
BlsI GCNGC 6 cut(s) 101, 104, 174, 322, 356, 517
Bme1390I CCNGG 1 cut(s) 314
BmeRI GACNNNNNGTC 2 cut(s) 127, 235
BmeT110I CYCGRG 1 cut(s) 671
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 1 cut(s) 289
Bpu14I TTCGAA 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 519
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 330
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 519
BseLI CCNNNNNNNGG 2 cut(s) 149, 330
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 3 cut(s) 75, 215, 713
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 5 cut(s) 86, 114, 159, 332, 502
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 2 cut(s) 9, 603
BsiHKCI CYCGRG 1 cut(s) 671
BslI CCNNNNNNNGG 2 cut(s) 149, 330
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 2 cut(s) 9, 603
BsoBI CYCGRG 1 cut(s) 671
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 3 cut(s) 68, 534, 563
BspACI CCGC 3 cut(s) 143, 273, 355
BspANI GGCC 2 cut(s) 9, 603
BspCNI CTCAG 3 cut(s) 74, 214, 712
BspMAI CTGCAG 2 cut(s) 104, 382
BspPI GGATC 1 cut(s) 571
BspT104I TTCGAA 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 3 cut(s) 68, 534, 563
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 741
BstAPI GCANNNNNTGC 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 5 cut(s) 164, 325, 359, 363, 605
BstDEI CTNAG 4 cut(s) 61, 201, 421, 699
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 2 cut(s) 80, 99
BstKTI GATC 3 cut(s) 71, 537, 566
BstMBI GATC 3 cut(s) 68, 534, 563
BstMWI GCNNNNNNNGC 2 cut(s) 159, 172
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 2 cut(s) 100, 378
BstV1I GCAGC 5 cut(s) 86, 114, 159, 332, 502
BsuRI GGCC 2 cut(s) 9, 603
BtsIMutI CAGTG 1 cut(s) 562
Cac8I GCNNGC 5 cut(s) 164, 325, 359, 363, 605
CfoI GCGC 2 cut(s) 80, 99
CseI GACGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 741
CviAII CATG 1 cut(s) 358
CviQI GTAC 1 cut(s) 741
DdeI CTNAG 4 cut(s) 61, 201, 421, 699
DpnI GATC 3 cut(s) 70, 536, 565
DpnII GATC 3 cut(s) 68, 534, 563
DriI GACNNNNNGTC 2 cut(s) 127, 235
Eam1104I CTCTTC 1 cut(s) 741
Eam1105I GACNNNNNGTC 2 cut(s) 127, 235
EarI CTCTTC 1 cut(s) 741
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 312
FaeI CATG 1 cut(s) 361
FaiI YATR 7 cut(s) 48, 58, 347, 359, 392, 614, 711
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 651, 683
FatI CATG 1 cut(s) 357
FauI CCCGC 1 cut(s) 150
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 6 cut(s) 100, 103, 173, 321, 355, 516
Fsp4HI GCNGC 6 cut(s) 100, 103, 173, 321, 355, 516
GlaI GCGC 2 cut(s) 79, 98
GluI GCNGC 6 cut(s) 100, 103, 173, 321, 355, 516
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 9, 603
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 80, 99
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 78, 97
HinP1I GCGC 2 cut(s) 78, 97
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 6 cut(s) 116, 122, 128, 374, 478, 626
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 388, 511, 625
Hpy188I TCNGA 8 cut(s) 121, 127, 133, 204, 241, 441, 568, 730
Hpy188III TCNNGA 3 cut(s) 63, 475, 574
Hpy8I GTNNAC 3 cut(s) 388, 511, 625
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 4 cut(s) 143, 428, 455, 765
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4V TGCA 6 cut(s) 102, 361, 380, 560, 593, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 172
HpyF3I CTNAG 4 cut(s) 61, 201, 421, 699
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 78, 97
Kzo9I GATC 3 cut(s) 68, 534, 563
LmnI GCTCC 2 cut(s) 451, 541
Lsp1109I GCAGC 5 cut(s) 86, 114, 159, 332, 502
LweI GCATC 1 cut(s) 289
MalI GATC 3 cut(s) 70, 536, 565
MboI GATC 3 cut(s) 68, 534, 563
MboII GAAGA 5 cut(s) 78, 308, 383, 758, 776
MluCI AATT 2 cut(s) 254, 597
MlyI GAGTC 4 cut(s) 116, 137, 487, 620
MmeI TCCRAC 2 cut(s) 111, 665
MspR9I CCNGG 1 cut(s) 314
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 159, 172
NdeII GATC 3 cut(s) 68, 534, 563
NlaIII CATG 1 cut(s) 361
NmeAIII GCCGAG 2 cut(s) 301, 544
NspI RCATGY 1 cut(s) 361
NspV TTCGAA 1 cut(s) 432
PaeI GCATGC 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 116, 374
PkrI GCNGC 6 cut(s) 101, 104, 174, 322, 356, 517
PleI GAGTC 4 cut(s) 116, 136, 486, 620
PpsI GAGTC 4 cut(s) 116, 136, 486, 620
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PstI CTGCAG 2 cut(s) 104, 382
RsaI GTAC 1 cut(s) 742
RsaNI GTAC 1 cut(s) 741
SalI GTCGAC 1 cut(s) 623
SatI GCNGC 6 cut(s) 100, 103, 173, 321, 355, 516
Sau3AI GATC 3 cut(s) 68, 534, 563
SchI GAGTC 4 cut(s) 116, 137, 487, 620
ScrFI CCNGG 1 cut(s) 314
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 2 cut(s) 100, 378
SfuI TTCGAA 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 1 cut(s) 361
Sse9I AATT 2 cut(s) 254, 597
SsiI CCGC 3 cut(s) 143, 273, 355
SspI AATATT 1 cut(s) 583
StyD4I CCNGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 557
TaqI TCGA 2 cut(s) 432, 624
TasI AATT 2 cut(s) 254, 597
TatI WGTACW 1 cut(s) 740
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 2 cut(s) 116, 374
TscAI CASTG 1 cut(s) 562
TseI GCWGC 5 cut(s) 99, 102, 172, 320, 515
TspDTI ATGAA 2 cut(s) 474, 622
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 1 cut(s) 562
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.