MD04G1164700.v1.1

At5g01610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009228
Physical Location & Seq
Reverse (-)
25521520 .. 25523492
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1164700.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGATCAGATTTTGAACAAGGCGGGATCCTACTGGTTCAACCAGAAGGCCACCAAGGAGCTCGGCTCCGTCGGTGATGACCTCAACTCATTGCAAAACAGTGTCGAAGGAGGAGCCAAATGGTTGGTTAATAAATTCAAAGGGAAAATGCAAAAGCCGTTGCCTGAGCTTCTAAAGGATTTTGACATGGCAATAGGAATCTTTCCTCGTGATGCTACCAACTATGAGTTTAATGAGGAGACTGGGAAAATTACTGTCTACATACCGACAGTCTGTGAAGTGGGCTACAGAGATTCGACTGTTTTACGTTTTTTCACCATTGTAACTGGATATTTGAAGAAAGGAAGGCTAGAAGATATACAGGGGATGAAGACAAAGGTGCTTATATGGGTAAATGTGTCATGCATCACATCGGAGGGATCGAAGCTCCATTTCACAGCTGGGTTGAAGAGAACCAGGAGTAGAGAGGCTTATGAGGTTCAAAGAGACGGAATTACTGTCGAGAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

171

Amino Acids

19.29

Weight (kDa)

8.98

Isoelectric Point (pI)

19.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 55 - 159 9.7e-26 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AciI CCGC 1 cut(s) 23
AclWI GGATC 4 cut(s) 12, 21, 34, 427
AcsI RAATTY 1 cut(s) 134
AgsI TTSAA 6 cut(s) 16, 40, 139, 337, 448, 482
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 4 cut(s) 61, 169, 427, 440
AluI AGCT 4 cut(s) 61, 169, 427, 440
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 233, 480
AlwI GGATC 4 cut(s) 12, 21, 34, 427
AoxI GGCC 1 cut(s) 48
ApoI RAATTY 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 86, 307
BamHI GGATCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 63
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 233, 480
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 286
Bme1390I CCNGG 1 cut(s) 457
BmiI GGNNCC 3 cut(s) 28, 67, 115
BmrFI CCNGG 1 cut(s) 457
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 202, 414
BmuI ACTGGG 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 54
Bse1I ACTGG 3 cut(s) 38, 247, 331
Bse3DI GCAATG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 372
BseMI GCAATG 1 cut(s) 89
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 38, 247, 331
BseRI GAGGAG 2 cut(s) 126, 251
BseYI CCCAGC 1 cut(s) 440
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 63
BsmAI GTCTC 2 cut(s) 233, 480
BsmBI CGTCTC 1 cut(s) 480
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 63
Bsp143I GATC 3 cut(s) 4, 26, 419
BspACI CCGC 1 cut(s) 23
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 28, 67, 115
BspPI GGATC 4 cut(s) 12, 21, 34, 427
BsrDI GCAATG 1 cut(s) 89
BsrI ACTGG 3 cut(s) 38, 247, 331
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 3 cut(s) 4, 26, 419
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 54
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 5 cut(s) 101, 256, 271, 301, 499
Bst6I CTCTTC 1 cut(s) 443
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 372
BstKTI GATC 3 cut(s) 7, 29, 422
BstMAI GTCTC 2 cut(s) 233, 480
BstMBI GATC 3 cut(s) 4, 26, 419
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 455
BstSFI CTRYAG 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 26
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 1 cut(s) 26
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 106
CviAII CATG 2 cut(s) 187, 402
DdeI CTNAG 1 cut(s) 165
DpnI GATC 3 cut(s) 6, 28, 421
DpnII GATC 3 cut(s) 4, 26, 419
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 407
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 480
FaeI CATG 2 cut(s) 190, 405
FaiI YATR 8 cut(s) 188, 225, 263, 359, 386, 388, 403, 474
FatI CATG 2 cut(s) 186, 401
FauI CCCGC 1 cut(s) 16
FblI GTMKAC 1 cut(s) 258
FokI GGATG 1 cut(s) 379
FriOI GRGCYC 1 cut(s) 63
FspBI CTAG 1 cut(s) 350
GsaI CCCAGC 1 cut(s) 444
HaeIII GGCC 1 cut(s) 50
Hin1II CATG 2 cut(s) 190, 405
HinfI GANTC 2 cut(s) 198, 293
HphI GGTGA 2 cut(s) 86, 307
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 2 cut(s) 9, 415
Hpy188III TCNNGA 2 cut(s) 209, 502
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 40, 101, 339
HpyCH4III ACNGT 5 cut(s) 101, 256, 271, 301, 499
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 94, 151, 405
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 2 cut(s) 190, 405
Kzo9I GATC 3 cut(s) 4, 26, 419
LmnI GCTCC 4 cut(s) 58, 71, 113, 432
LpnPI CCDG 9 cut(s) 19, 56, 177, 228, 312, 347, 426, 442, 469
LweI GCATC 2 cut(s) 202, 414
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 6, 28, 421
MboI GATC 3 cut(s) 4, 26, 419
MboII GAAGA 4 cut(s) 349, 365, 382, 460
MflI RGATCY 1 cut(s) 26
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 3 cut(s) 134, 249, 492
MnlI CCTC 7 cut(s) 92, 104, 216, 229, 409, 460, 469
Mph1103I ATGCAT 1 cut(s) 407
MseI TTAA 3 cut(s) 129, 231, 511
MspA1I CMGCKG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 457
NdeII GATC 3 cut(s) 4, 26, 419
NlaIII CATG 2 cut(s) 190, 405
NlaIV GGNNCC 3 cut(s) 28, 67, 115
NmeAIII GCCGAG 1 cut(s) 42
NsiI ATGCAT 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PfeI GAWTC 2 cut(s) 198, 293
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 28, 67, 115
PsuI RGATCY 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 129, 231, 511
Sau3AI GATC 3 cut(s) 4, 26, 419
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 1 cut(s) 63
SetI ASST 8 cut(s) 63, 84, 171, 310, 381, 429, 442, 480
SfaNI GCATC 2 cut(s) 202, 414
SfcI CTRYAG 1 cut(s) 286
Sse9I AATT 3 cut(s) 134, 249, 492
SsiI CCGC 1 cut(s) 23
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 455
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 5 cut(s) 101, 256, 271, 301, 499
TaiI ACGT 1 cut(s) 310
TaqI TCGA 4 cut(s) 105, 296, 422, 501
TasI AATT 3 cut(s) 134, 249, 492
TfiI GAWTC 2 cut(s) 198, 293
Tru1I TTAA 3 cut(s) 129, 231, 511
Tru9I TTAA 3 cut(s) 129, 231, 511
TscAI CASTG 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 2 cut(s) 58, 504
TspRI CASTG 1 cut(s) 106
XapI RAATTY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.