RLG00000024898

At5g01610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009228
Physical Location & Seq
Reverse (-)
40130907 .. 40132491
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024898

Sequence Viewer

Length: 516 bp
ATGGATCAGATTCTGAACAAGGCGGGGTCCTACTGGTTCAGCCAGAAGGCCACCAAGGAGATCTCCTCCGTCGGCGATGACATCAACTCATTGCAATCCAGCATTTCAGGAGGAGCCAGTTGGTTGGTTAATAAGGTCAAAGGAAGCATGCAAAAGCCATTACCTGAGCTTCTTAAGGAGTATGACCTCGCAGTAGGAATCTTTCCTCGGGATGCTACAAACTATGAATTCAATCAGGAGACAGGAAAACTTACCGTCTACATACCCTCAGTCTGTGAAGTGGGCTATAGGGATTCGTCTGTCTTGCGGTTTTTCACGACTGTAACTGGATACTTGAAGAAAGGAAAGCTAGAAGACATTCAGGGGATCAAGACAAAGGTGACCTTACTTTGGGTAAAAGTGACCTCTATCTCGTCTGGGGGATCGAAGCTTAATTTCCAGGCCGGGATGAACAGAAGCAGGAGTAGAGAGGCTTATGAGGTTTCTAGAGATGGAATTACCGTAGACAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

172

Amino Acids

18.98

Weight (kDa)

9.23

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 55 - 160 3.7e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 258, 504
AciI CCGC 2 cut(s) 23, 307
AclWI GGATC 3 cut(s) 12, 374, 430
AcsI RAATTY 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 390
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 232, 337
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 3 cut(s) 169, 349, 430
AluI AGCT 3 cut(s) 169, 349, 430
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 3 cut(s) 12, 374, 430
AlwNI CAGNNNCTG 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 48, 441
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 360
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 1 cut(s) 440
BciVI GTATCC 1 cut(s) 323
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 2 cut(s) 350, 486
BfmI CTRYAG 1 cut(s) 286
BfrI CTTAAG 1 cut(s) 173
BfuI GTATCC 1 cut(s) 323
BglII AGATCT 1 cut(s) 60
Bme1390I CCNGG 2 cut(s) 440, 445
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 28, 115
BmrFI CCNGG 2 cut(s) 440, 445
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 445
BsaJI CCNNGG 2 cut(s) 54, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse1I ACTGG 3 cut(s) 38, 117, 331
Bse3DI GCAATG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 54, 206
BseGI GGATG 2 cut(s) 217, 453
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMI GCAATG 1 cut(s) 89
BseMII CTCAG 2 cut(s) 156, 282
BseNI ACTGG 3 cut(s) 38, 117, 331
BseRI GAGGAG 2 cut(s) 55, 126
BshFI GGCC 2 cut(s) 50, 443
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 2 cut(s) 50, 443
BsoBI CYCGRG 1 cut(s) 207
Bsp143I GATC 4 cut(s) 4, 60, 366, 422
BspACI CCGC 2 cut(s) 23, 307
BspANI GGCC 2 cut(s) 50, 443
BspCNI CTCAG 2 cut(s) 157, 281
BspLI GGNNCC 2 cut(s) 28, 115
BspPI GGATC 3 cut(s) 12, 374, 430
BspTI CTTAAG 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 89
BsrI ACTGG 3 cut(s) 38, 117, 331
BssECI CCNNGG 2 cut(s) 54, 206
BssMI GATC 4 cut(s) 4, 60, 366, 422
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 3 cut(s) 256, 322, 502
BstAFI CTTAAG 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 2 cut(s) 165, 268
BstEII GGTNACC 1 cut(s) 379
BstF5I GGATG 2 cut(s) 217, 453
BstKTI GATC 4 cut(s) 7, 63, 369, 425
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 4 cut(s) 4, 60, 366, 422
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 438, 443
BstSFI CTRYAG 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 1 cut(s) 60
BsuI GTATCC 1 cut(s) 323
BsuRI GGCC 2 cut(s) 50, 443
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 217, 453
Cac8I GCNNGC 1 cut(s) 149
CaiI CAGNNNCTG 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 27
CviAII CATG 1 cut(s) 148
DdeI CTNAG 2 cut(s) 165, 268
DpnI GATC 4 cut(s) 6, 62, 368, 424
DpnII GATC 4 cut(s) 4, 60, 366, 422
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 27
EcoO65I GGTNACC 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 1 cut(s) 151
FaiI YATR 6 cut(s) 149, 183, 225, 263, 288, 477
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FatI CATG 1 cut(s) 147
FauI CCCGC 1 cut(s) 16
FblI GTMKAC 2 cut(s) 258, 504
FokI GGATG 2 cut(s) 224, 460
FspBI CTAG 2 cut(s) 350, 486
HaeIII GGCC 2 cut(s) 50, 443
HapII CCGG 1 cut(s) 444
Hin1II CATG 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 3 cut(s) 10, 198, 293
HpaII CCGG 1 cut(s) 444
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 2 cut(s) 259, 505
Hpy188I TCNGA 2 cut(s) 9, 15
Hpy188III TCNNGA 6 cut(s) 108, 209, 236, 316, 370, 486
Hpy8I GTNNAC 2 cut(s) 259, 505
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 3 cut(s) 256, 322, 502
HpyCH4V TGCA 2 cut(s) 94, 151
HpyF3I CTNAG 2 cut(s) 165, 268
Hsp92II CATG 1 cut(s) 151
Kzo9I GATC 4 cut(s) 4, 60, 366, 422
LmnI GCTCC 1 cut(s) 113
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 350, 486
MaeIII GTNAC 3 cut(s) 322, 379, 400
MalI GATC 4 cut(s) 6, 62, 368, 424
MboI GATC 4 cut(s) 4, 60, 366, 422
MboII GAAGA 2 cut(s) 349, 365
MflI RGATCY 1 cut(s) 60
MluCI AATT 3 cut(s) 227, 433, 495
MnlI CCTC 8 cut(s) 76, 104, 197, 216, 277, 415, 463, 472
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 4 cut(s) 129, 174, 432, 514
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 2 cut(s) 440, 445
MvaI CCWGG 1 cut(s) 440
NciI CCSGG 1 cut(s) 445
NdeII GATC 4 cut(s) 4, 60, 366, 422
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 2 cut(s) 28, 115
NmuCI GTSAC 2 cut(s) 379, 400
NspI RCATGY 1 cut(s) 151
PaeI GCATGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 10, 198, 293
PpuMI RGGWCCY 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 379
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 28, 115
PspPI GGNCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 27
PstNI CAGNNNCTG 1 cut(s) 13
PsuI RGATCY 1 cut(s) 60
SaqAI TTAA 4 cut(s) 129, 174, 432, 514
Sau3AI GATC 4 cut(s) 4, 60, 366, 422
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 2 cut(s) 440, 445
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 1 cut(s) 286
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
SphI GCATGC 1 cut(s) 151
Sse9I AATT 3 cut(s) 227, 433, 495
SsiI CCGC 2 cut(s) 23, 307
SspMI CTAG 2 cut(s) 350, 486
StyD4I CCNGG 2 cut(s) 438, 443
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 3 cut(s) 256, 322, 502
TaqI TCGA 1 cut(s) 425
TasI AATT 3 cut(s) 227, 433, 495
TfiI GAWTC 3 cut(s) 10, 198, 293
Tru1I TTAA 4 cut(s) 129, 174, 432, 514
Tru9I TTAA 4 cut(s) 129, 174, 432, 514
TseFI GTSAC 2 cut(s) 379, 400
Tsp45I GTSAC 2 cut(s) 379, 400
TspDTI ATGAA 2 cut(s) 240, 464
TspGWI ACGGA 1 cut(s) 58
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 485
XceI RCATGY 1 cut(s) 151
XmiI GTMKAC 2 cut(s) 258, 504
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 350, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.