Rorug03G0053600

At5g01610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009228
Physical Location & Seq
Forward (+)
4147618 .. 4150377
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0053600.1

Sequence Viewer

Length: 1755 bp
ATGAATAAGCAAGACTTCATGAAGATTCAGAGCTGTGTTCTCAGAGTGAACATTCACTGTGAAGGTTGTAAGCAGAAAGTTAAGAAATTGGTGCAGAAAATTGATGGTGTGTTCACTACTAGTATAGATGCAGAGCAAGGCAAAGTGACTGTCACAGGGAACGTAGATCCAGCCACAATCATCAAGAAGCTTGAAAAGTCAGGCAAACATGCAGAGCTTTGGGGAGGGGGAGGAGGAGGAGGAGGACAGAAGGGTAATTCCCAAGCGCAGCAGTTCAAGAACATGTCAATGGAGAATCATGGCAAAGGGGGAAAAGACAATGGTAGTAACAAGGCTCAGAAGGGTGGTGGTGGTGGTGGAAACCAACAGAAGGGCAATAATGTCCAGCAACAAATTCAGCAGCAACAGCAGATGATGAAAGGGGCTAAGGTGATGATGCCAACGAAGGAGCAGAAAAAGTCTGTTAAGTTCACTTTGCCTGCTGAGGAGGAGTTTAGTGATGGTGAGTTTGATGATGAGTTTGATGATGATGATTATGATGATGATGAGTTTGATGATGAGGATTATGAAGATGATGAGGAGGAAGAGCTTAACCATGGCCATCAGGTACCACACAACAAGATGATGATGATGCCTGGTATGGGTGCAAATGGGCCACAAGGGCCTTATGGGAAGATGATGCCTGTGATGGGTAATAATGGACATGGGCCACACGGGCCGGGTGGGATGGTCAATATGAATGCTATGGGCGGCAAGAAGTTAGGTGGTGGTGGTGGGGGAGGAGGGTCTGTCAAGAAAGGTGAGGTTATTGATTTCCCAATGCAAGGGATGAAGGGCATGGGTGGAAATAAGGTAAAGAAGGATGGCAAAGATGGCAAGGGAGGAAAGAAAGGGGGAGGAGATGATGAGAAGGGCAAGAGCAAGAAAAAGAGCAAGGGGGGTAAAGATGGTAAAGGAGGTAAAGACAAAAGTGAGGGTAAAAAGGGTGGGTTGCTTGGGTGGCTTAGCAGAAGCACCAGTAAAAAAGAGAGTGATGGAAACCACAAGGGGGGCAAGGGAGGTAAAAAAGGTGGTGACAAGTATGATGATTTCATGGAAATTGATGTCATGAATCATAAGAAAGGAGGCAAAGAAGGTAAAGAAAGCAAGGAAAGCAAAGAAGGCAAAGGAAGCAAAGGAGGCAAAGGTGGTGGTGGCAATGGTGGAGATGTTCATCAGATGGGTCAAAAGGGTCAAATGGGCCAGGCAGGTCAGATGGGGCATATGGGCCAAATGGGTCAAATGGGCAATATGGGTCAGATGGGCCATATGGGGCAGATGAGGCCAATGGGCCAAATGGGGCGTGGAGGTCAGAATATGGGCCAGATGGGTAACTACCCAATGGGCAGTATGGGTCAGATGGGCAATGTGCCTGCAGTACAAGGACTACCTGCACCGGGGATGATGAACGGTGGCGGTGGTGGTGGCTATTATCAAGGGATGGGAAATGCCGGAAATCCCTATACTCAACAGCAGCTCCAGCAACAGCAACAGCAACAACAGCTCCAGCAGCAGCAATATATGGCTATGATGATGAACCAGCAACAGCAGCAGCAAAGGGCTAATGGGAATGACATGTACCAACCCATGATGTATGCGCGGCCACAGCCACCGATGAACTACATGCCCCATCAGCCAATGCCTTCAGCAGCGCCATCTGAGCCTTACACTAACTTCTTCAGTGATGAGAATGCCAACAGTTGCAGCGTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

584

Amino Acids

62.18

Weight (kDa)

8.98

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 14 - 70 5.6e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1238, 1438
Acc65I GGTACC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 1 cut(s) 1639
AciI CCGC 3 cut(s) 750, 1455, 1639
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 2 cut(s) 598, 1640
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 2 cut(s) 1668, 1702
AfaI GTAC 3 cut(s) 609, 1419, 1619
AfiI CCNNNNNNNGG 6 cut(s) 370, 641, 689, 824, 1048, 1436
AflIII ACRYGT 2 cut(s) 282, 1614
AgsI TTSAA 2 cut(s) 194, 277
AhlI ACTAGT 1 cut(s) 119
AjnI CCWGG 2 cut(s) 634, 1242
AluBI AGCT 6 cut(s) 33, 190, 217, 589, 1516, 1543
AluI AGCT 6 cut(s) 33, 190, 217, 589, 1516, 1543
AlwI GGATC 1 cut(s) 161
ApeKI GCWGC 9 cut(s) 268, 400, 1513, 1549, 1552, 1588, 1591, 1688, 1743
ApoI RAATTY 1 cut(s) 393
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
Asp718I GGTACC 1 cut(s) 607
AspLEI GCGC 3 cut(s) 268, 1639, 1693
AspS9I GGNCC 9 cut(s) 653, 662, 707, 716, 1240, 1267, 1303, 1330, 1360
AsuC2I CCSGG 2 cut(s) 720, 1437
AsuHPI GGTGA 4 cut(s) 442, 515, 812, 1085
BalI TGGCCA 1 cut(s) 600
BanI GGYRCC 1 cut(s) 607
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 8 cut(s) 280, 412, 1525, 1561, 1564, 1600, 1603, 1700
BciT130I CCWGG 2 cut(s) 636, 1244
BcnI CCSGG 2 cut(s) 720, 1437
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 1413
BfoI RGCGCY 1 cut(s) 1694
BfuAI ACCTGC 2 cut(s) 1238, 1438
BglI GCCNNNNNGGC 2 cut(s) 661, 715
BlpI GCTNAGC 1 cut(s) 1004
Bme1390I CCNGG 4 cut(s) 636, 720, 1244, 1437
BmgT120I GGNCC 9 cut(s) 653, 662, 707, 716, 1240, 1267, 1303, 1330, 1360
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 4 cut(s) 636, 720, 1244, 1437
BmsI GCATC 4 cut(s) 118, 426, 621, 669
BpmI CTGGAG 2 cut(s) 1502, 1529
Bpu10I CCTNAGC 2 cut(s) 426, 483
Bpu1102I GCTNAGC 1 cut(s) 1004
BpuMI CCSGG 2 cut(s) 720, 1437
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 2 cut(s) 595, 1436
BsaXI ACNNNNNCTCC 6 cut(s) 774, 804, 1500, 1527, 1530, 1557
Bsc4I CCNNNNNNNGG 6 cut(s) 370, 641, 689, 824, 1048, 1436
Bse1I ACTGG 1 cut(s) 1017
Bse3DI GCAATG 2 cut(s) 1204, 1411
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 636, 1244
BseDI CCNNGG 2 cut(s) 595, 1436
BseGI GGATG 5 cut(s) 732, 834, 868, 1446, 1485
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 6 cut(s) 370, 641, 689, 824, 1048, 1436
BseMI GCAATG 2 cut(s) 1204, 1411
BseMII CTCAG 4 cut(s) 55, 350, 474, 1689
BseNI ACTGG 1 cut(s) 1017
BseRI GAGGAG 9 cut(s) 246, 249, 252, 255, 500, 503, 593, 795, 912
BseXI GCAGC 8 cut(s) 280, 412, 1525, 1561, 1564, 1600, 1603, 1700
BsgI GTGCAG 2 cut(s) 113, 1416
Bsh1236I CGCG 1 cut(s) 1639
BshNI GGYRCC 1 cut(s) 607
BsiSI CCGG 3 cut(s) 719, 1436, 1491
BslI CCNNNNNNNGG 6 cut(s) 370, 641, 689, 824, 1048, 1436
BsmI GAATGC 2 cut(s) 745, 1735
Bsp143I GATC 1 cut(s) 166
Bsp1720I GCTNAGC 1 cut(s) 1004
Bsp19I CCATGG 1 cut(s) 595
BspACI CCGC 3 cut(s) 750, 1455, 1639
BspCNI CTCAG 4 cut(s) 54, 349, 475, 1690
BspFNI CGCG 1 cut(s) 1639
BspHI TCATGA 2 cut(s) 18, 1107
BspLI GGNNCC 1 cut(s) 609
BspMAI CTGCAG 1 cut(s) 1417
BspMI ACCTGC 2 cut(s) 1238, 1438
BspPI GGATC 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 607
BsrDI GCAATG 2 cut(s) 1204, 1411
BsrI ACTGG 1 cut(s) 1017
BssECI CCNNGG 2 cut(s) 595, 1436
BssMI GATC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 595
Bst2UI CCWGG 2 cut(s) 636, 1244
Bst4CI ACNGT 4 cut(s) 59, 151, 1451, 1739
Bst6I CTCTTC 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 480, 1413
BstDEI CTNAG 6 cut(s) 41, 336, 426, 483, 1004, 1698
BstDSI CCRYGG 1 cut(s) 595
BstF5I GGATG 5 cut(s) 732, 834, 868, 1446, 1485
BstFNI CGCG 1 cut(s) 1639
BstH2I RGCGCY 1 cut(s) 1694
BstHHI GCGC 3 cut(s) 268, 1639, 1693
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstNI CCWGG 2 cut(s) 636, 1244
BstNSI RCATGY 4 cut(s) 212, 286, 1618, 1666
BstSCI CCNGG 4 cut(s) 634, 718, 1242, 1435
BstSFI CTRYAG 1 cut(s) 1413
BstUI CGCG 1 cut(s) 1639
BstV1I GCAGC 8 cut(s) 280, 412, 1525, 1561, 1564, 1600, 1603, 1700
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BtgI CCRYGG 1 cut(s) 595
BtsCI GGATG 5 cut(s) 732, 834, 868, 1446, 1485
BtsIMutI CAGTG 2 cut(s) 55, 1726
BveI ACCTGC 2 cut(s) 1238, 1438
Cac8I GCNNGC 2 cut(s) 480, 1413
CciI TCATGA 2 cut(s) 18, 1107
CfoI GCGC 3 cut(s) 268, 1639, 1693
Cfr13I GGNCC 9 cut(s) 653, 662, 707, 716, 1240, 1267, 1303, 1330, 1360
CseI GACGC 1 cut(s) 1735
Csp6I GTAC 3 cut(s) 608, 1418, 1618
CspCI CAANNNNNGTGG 2 cut(s) 1171, 1206
CviQI GTAC 3 cut(s) 608, 1418, 1618
DdeI CTNAG 6 cut(s) 41, 336, 426, 483, 1004, 1698
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 2 cut(s) 598, 1640
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 595
Eco57I CTGAAG 2 cut(s) 1668, 1702
EcoO109I RGGNCCY 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 634, 1242
EcoT14I CCWWGG 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 595
FalI AAGNNNNNCTT 1 cut(s) 31
FauNDI CATATG 2 cut(s) 1263, 1308
FokI GGATG 5 cut(s) 739, 841, 875, 1453, 1492
FspBI CTAG 1 cut(s) 120
GlaI GCGC 3 cut(s) 267, 1638, 1692
GsuI CTGGAG 2 cut(s) 1502, 1529
HaeII RGCGCY 1 cut(s) 1694
HapII CCGG 3 cut(s) 719, 1436, 1491
HgaI GACGC 1 cut(s) 1735
HhaI GCGC 3 cut(s) 268, 1639, 1693
Hin6I GCGC 3 cut(s) 266, 1637, 1691
HinP1I GCGC 3 cut(s) 266, 1637, 1691
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 3 cut(s) 25, 295, 1111
HpaII CCGG 3 cut(s) 719, 1436, 1491
HphI GGTGA 4 cut(s) 442, 515, 812, 1085
Hpy166II GTNNAC 3 cut(s) 49, 114, 471
Hpy188I TCNGA 9 cut(s) 30, 44, 339, 1218, 1254, 1299, 1353, 1398, 1699
Hpy188III TCNNGA 5 cut(s) 19, 184, 277, 793, 1108
Hpy8I GTNNAC 3 cut(s) 49, 114, 471
HpyCH4III ACNGT 4 cut(s) 59, 151, 1451, 1739
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 8 cut(s) 94, 131, 212, 647, 823, 1415, 1433, 1743
HpyF3I CTNAG 6 cut(s) 41, 336, 426, 483, 1004, 1698
HpySE526I ACGT 1 cut(s) 162
HspAI GCGC 3 cut(s) 266, 1637, 1691
KpnI GGTACC 1 cut(s) 611
Kzo9I GATC 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 3 cut(s) 448, 1521, 1548
Lsp1109I GCAGC 8 cut(s) 280, 412, 1525, 1561, 1564, 1600, 1603, 1700
LweI GCATC 4 cut(s) 118, 426, 621, 669
MaeI CTAG 1 cut(s) 120
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 5 cut(s) 145, 151, 326, 1073, 1370
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 5 cut(s) 34, 581, 596, 685, 1708
MflI RGATCY 1 cut(s) 166
MlsI TGGCCA 1 cut(s) 600
MluCI AATT 5 cut(s) 86, 99, 256, 393, 1098
MluNI TGGCCA 1 cut(s) 600
Mox20I TGGCCA 1 cut(s) 600
MscI TGGCCA 1 cut(s) 600
MseI TTAA 3 cut(s) 81, 465, 591
MslI CAYNNNNRTG 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 600
MspI CCGG 3 cut(s) 719, 1436, 1491
MspR9I CCNGG 4 cut(s) 636, 720, 1244, 1437
Mva1269I GAATGC 2 cut(s) 745, 1735
MvaI CCWGG 2 cut(s) 636, 1244
MvnI CGCG 1 cut(s) 1639
NciI CCSGG 2 cut(s) 720, 1437
NcoI CCATGG 1 cut(s) 595
NdeI CATATG 2 cut(s) 1263, 1308
NdeII GATC 1 cut(s) 166
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 3 cut(s) 145, 151, 1073
NspI RCATGY 4 cut(s) 212, 286, 1618, 1666
PagI TCATGA 2 cut(s) 18, 1107
PciI ACATGT 2 cut(s) 282, 1614
PciSI GCTCTTC 1 cut(s) 579
PctI GAATGC 2 cut(s) 745, 1735
PfeI GAWTC 3 cut(s) 25, 295, 1111
PscI ACATGT 2 cut(s) 282, 1614
Psp6I CCWGG 2 cut(s) 634, 1242
PspGI CCWGG 2 cut(s) 634, 1242
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 9 cut(s) 653, 662, 707, 716, 1240, 1267, 1303, 1330, 1360
PstI CTGCAG 1 cut(s) 1417
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 3 cut(s) 609, 1419, 1619
RsaNI GTAC 3 cut(s) 608, 1418, 1618
RseI CAYNNNNRTG 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 81, 465, 591
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 9 cut(s) 653, 662, 707, 716, 1240, 1267, 1303, 1330, 1360
ScrFI CCNGG 4 cut(s) 636, 720, 1244, 1437
SfaNI GCATC 4 cut(s) 118, 426, 621, 669
SfcI CTRYAG 1 cut(s) 1413
SfiI GGCCNNNNNGGCC 2 cut(s) 661, 715
SmiMI CAYNNNNRTG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 119
Sse9I AATT 5 cut(s) 86, 99, 256, 393, 1098
SsiI CCGC 3 cut(s) 750, 1455, 1639
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 634, 718, 1242, 1435
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 4 cut(s) 59, 151, 1451, 1739
TaiI ACGT 1 cut(s) 165
TasI AATT 5 cut(s) 86, 99, 256, 393, 1098
TatI WGTACW 1 cut(s) 1417
TauI GCSGC 2 cut(s) 753, 1642
TfiI GAWTC 3 cut(s) 25, 295, 1111
Tru1I TTAA 3 cut(s) 81, 465, 591
Tru9I TTAA 3 cut(s) 81, 465, 591
TscAI CASTG 2 cut(s) 62, 1726
TseFI GTSAC 3 cut(s) 145, 151, 1073
TseI GCWGC 9 cut(s) 268, 400, 1513, 1549, 1552, 1588, 1591, 1688, 1743
Tsp45I GTSAC 3 cut(s) 145, 151, 1073
TspRI CASTG 2 cut(s) 62, 1726
XapI RAATTY 1 cut(s) 393
XceI RCATGY 4 cut(s) 212, 286, 1618, 1666
XcmI CCANNNNNNNNNTGG 1 cut(s) 1340
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.