Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014426

Physical Location & Seq

Genomic Coordinates Forward (+)

27256030 .. 27259385

Gene Structure 3356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1181500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGCTGCTTGTGCTCTCCTCGGTGACAATGTCGGCGACCACATCAGCGGCAATGGCAGCAATAACAACAACATCAGTAGCCACGGCGGCGGTGGAGGAAGCCCTTCTTGTCCTATGAACAACAGCACTACCAATTCCAACAGCAGCAGCGTCGAGCAGCAGCCAACCAAGATGGTGAGAAAGCGCATGGCGTCCGAGATCGAAGTCCAAACCACCCCGACAAGCAGAACCGCCGGTTCGGACTATTTCCGGCTCTCCCGCCGCGGGGTTATTAATTATAGTCCGCCAGTCCAGCCGGTATATCCAAACCCAAATCAAAATCCAAACCCCAGTAAAGTGAATAGTAGTATGTTTTATCCCAACTACTCCACTATGCTGTTACCCGTACCATCTTCCACAAATTTGACCGCTTTGACGTCAGCTGGTGGGGCTTTATTGTCACCTGCTTCTCCTTCTCCTTCCCCTTCTCCTTCTCCTTCTTCCGCTGCAGCAGCTGGCAGCTGGGGTCCTATCGATCCATTGTCGTTTCATCGCCCTCTTTCGATTCAGCCGACAACGACGTCCACCCAGATCACTACCACTCCGGCCGTTTGTGGGTTTTCTGGTCTGCCCTTGTTCCCACCAGAAAAGACTCCTCCTTCGAATCCTACTACCGCTGCTACTACTATTTCCGCCATGGAAGACGGCTCCTCTGCCGCCACGGCATGGATAGACGGGATCATAAAGGACCTCATCCACAGCTCCACCAACGTCTCCATCCCCCAGCTCATTCACAATGTTAGGGAGATTATCTTCCCTTGCAACCCCAGCCTCGCGGCGCTCCTCGAGTACAGACTCCGGTCCATATCCGAGCCGCCGCCTCCTCTGCCTCTTCCAGTCCCGAGCTTTAATCCGACCCCAATCCCGGACATGAGGAGGAGACAACAAGGTGGTCCGGGCCCTGGGGCTCTTAAGATCAATCTGGACTCTGGTGCCCTACATGACGTCGCAATCTTCAACACATCAACGGCGGATAACAATGACTTGTACTTGCAGTCGTGGTCTGGAGGTGGCGGAGGTGGCGGAGGGGTTGGGCTGTCCCTACCCCAACCCAACCCTACTCCTGTCCCTATGACCTGCAACCAAACAAACCCACATCACCAAAGCCCTTCCTTTAATCAGACAATCCATCAAACACAAGACAAACAAATAGAAAACTCATCATCCTCTTCCCCTGCGGCGGAGTCCACCACCCCAACCGCTGCACCCGCACCCACAACCGCTACCGCCACAGCCCCGCCGCAACCAACAACCTCAGCAGTCTCCCTACTCAGAGAGAAAAAAGAAGAGATGCGGCAGCAAAAGCGAGACGAGGAAGGCCTACACCTCCTCACCCTCCTCCTCCAATGCGCGGAGGCGGTCTCCGCAGACAACTACGACGAAGCCACCAAAATGCTCCTCGAAATTTCCGAGCTCTCCACCCCATTCGGCACCTCCGCCCAGCGCGTCGCGGCCTACTTCTCCGAAGCCATGTCAGCCCGCCTCGTCAGCTCCTGCCTCGGCATCTACGCCTCCCTCCCTCACTCCTCCGTCCCCATCACCTACACCCAAAAAATGGTCTCCGCCTTCCAAGTCTTCAACGGCATCAGCCCCTTCGTCAAGTTTTCCCACTTCACCGCCAATCAGGCGATCCAGGAGGCGTTCGAGGGGGAGGACAGGGTCCACATCGTCGATCTCGACATTATGCAGGGCCTCCAATGGCCCGGGCTGTTCCACATCCTGGCCTCCAGACCCGGTGGTCCTCCATACGTCAGGCTCACCGGGCTCGGGACCTCAATGGAGGCCTTGGAGGCCACGGGGAAGCGGTTGTCCGATTTCGCAGACAAGCTAGGGCTTCCGTTCGAGTTCTTCCCCGTGGCGGAGAAAGTTGGGAACTTGGACCCGGAGAGGCTGAATATCAGCAAGAGAGAGGCTGTGGCGGTGCACTGGCTGCAGCATTCGCTTTACGATATCACGGGTTCCGATTCCAACACGCTCTGGCTTTTGCAGAGATTGGCGCCAAAAGTGGTAACGGTGGTGGAGCAAGACCTGAGCCACGCGGGCTCGTTCTTGGGCCGGTTTGTGGAGGCCATACACTACTACTCTGCCCTGTTTGATTCCTTGGGAGCGAGCTACAGCGAAGAGAGCGAGGAGAGGCACGTGGTGGAGCAGCAGCTGCTCTCTAGAGAGATTCGTAACGTTTTAGCAGTGGGCGGGCCGTCGAGGAGCGGAGAGGTGAAGTTCCACAATTGGAGGGAGAAGTTTCAGCAGAGCGGGTTTCGGGGCATTTCCCTGGCGGGAAATGCGGCAACTCAGGCCACCTTGCTGCTCGGGATGTTCCCTTCCGACGGGTACACTCTGGTGGAGGACAATGGGACTCTCAAGCTTGGGTGGAAGGATCTCTGCTTGCTCACCGCTTCCGCATGGCGGCCGCCGTTTCATGCCGCCGCCAATCCGAACCTTCACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008356 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009889 GO:0009956 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032507 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045165 GO:0045185 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051235 GO:0051252 GO:0051301 GO:0051457 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0072595 GO:0080090 GO:0090610 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

830

Amino Acids

88.69

Weight (kDa)

5.88

Isoelectric Point (pI)

60.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	457 - 816	1.5e-129	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014426)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54220
fragaria_vesca	FvH4_6g09040
malus_domestica	MD04G1181500.v1.1 MD12G1196300.v1.1
prunus_persica	Prupe.6G300900_v2.0.a1
pyrus_communis	pycom04g16040 pycom12g18250
rosa_chinensis	RchiOBHm_Chr3g0459291
rosa_laevigata	RLG00000025096
rosa_multiflora	Rmu_sc0012999.1_g000002
rosa_roxburghii	Rroxscaffold_6G00420330
rosa_rugosa	Rorug03G0035100
rosa_samantha	Rh3AG092900 Rh3BG096000 Rh3CG097000 Rh3DG096600
rosa_wichuraiana	Rw3G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	451
AasI	GACNNNNNNGTC	2 cut(s)	559, 1776
AatII	GACGTC	3 cut(s)	419, 563, 987
Acc36I	ACCTGC	2 cut(s)	451, 1124
AccB1I	GGYRCC	3 cut(s)	971, 1469, 2035
AccB7I	CCANNNNNTGG	3 cut(s)	705, 1594, 1759
AccBSI	CCGCTC	2 cut(s)	2247, 2292
AccII	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
AclI	AACGTT	1 cut(s)	2217
AclWI	GGATC	4 cut(s)	509, 725, 1663, 2424
AcoI	YGGCCR	2 cut(s)	585, 2447
AcsI	RAATTY	2 cut(s)	400, 1443
AcvI	CACGTG	1 cut(s)	2179
AcyI	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
AdeI	CACNNNGTG	1 cut(s)	2182
AfaI	GTAC	4 cut(s)	387, 830, 1028, 2372
AflII	CTTAAG	1 cut(s)	950
AgsI	TTSAA	2 cut(s)	997, 1618
AjnI	CCWGG	4 cut(s)	940, 1671, 1758, 2310
AleI	CACNNNNGTG	1 cut(s)	2378
Alw21I	GWGCWC	3 cut(s)	16, 1455, 1965
Alw26I	GTCTC	6 cut(s)	757, 913, 1306, 1341, 1405, 1603
Alw44I	GTGCAC	1 cut(s)	1961
AlwI	GGATC	4 cut(s)	509, 725, 1663, 2424
AlwNI	CAGNNNCTG	3 cut(s)	494, 1533, 2194
Ama87I	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
ApaI	GGGCCC	1 cut(s)	941
ApaLI	GTGCAC	1 cut(s)	1961
ApoI	RAATTY	2 cut(s)	400, 1443
ArsI	GACNNNNNNTTYG	2 cut(s)	622, 654
AseI	ATTAAT	1 cut(s)	273
Asp700I	GAANNNNTTC	1 cut(s)	103
AspLEI	GCGC	5 cut(s)	186, 820, 1391, 1485, 2038
AsuC2I	CCSGG	7 cut(s)	905, 936, 1743, 1744, 1773, 1801, 1922
AsuII	TTCGAA	1 cut(s)	641
AvaI	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
AvaII	GGWCC	8 cut(s)	506, 727, 840, 932, 1699, 1778, 1809, 1918
BaeGI	GKGCMC	3 cut(s)	941, 976, 1965
BanI	GGYRCC	3 cut(s)	971, 1469, 2035
BanII	GRGCYC	5 cut(s)	941, 949, 1455, 1806, 2084
BbrPI	CACGTG	1 cut(s)	2179
BbsI	GAAGAC	2 cut(s)	687, 1606
Bbv12I	GWGCWC	3 cut(s)	16, 1455, 1965
BbvCI	CCTCAGC	1 cut(s)	1294
BccI	CCATC	5 cut(s)	166, 397, 764, 1176, 1583
BceAI	ACGGC	8 cut(s)	100, 572, 700, 717, 1023, 1636, 2221, 2437
BcgI	CGANNNNNNTGC	2 cut(s)	133, 167
BciT130I	CCWGG	4 cut(s)	942, 1673, 1760, 2312
BcnI	CCSGG	7 cut(s)	905, 936, 1743, 1744, 1773, 1801, 1922
BcoDI	GTCTC	6 cut(s)	757, 913, 1306, 1341, 1405, 1603
BfaI	CTAG	2 cut(s)	1868, 2202
BfmI	CTRYAG	3 cut(s)	486, 1970, 2152
BfoI	RGCGCY	2 cut(s)	821, 2039
BfrI	CTTAAG	1 cut(s)	950
BfuAI	ACCTGC	2 cut(s)	451, 1124
BglI	GCCNNNNNGGC	5 cut(s)	87, 701, 1664, 1829, 2079
Bme18I	GGWCC	8 cut(s)	506, 727, 840, 932, 1699, 1778, 1809, 1918
BmeT110I	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
BmrI	ACTGGG	1 cut(s)	324
BmsI	GCATC	3 cut(s)	1320, 1551, 1632
BmuI	ACTGGG	1 cut(s)	324
BoxI	GACNNNNGTC	1 cut(s)	838
BpiI	GAAGAC	2 cut(s)	687, 1606
BplI	GAGNNNNNCTC	2 cut(s)	1385, 1417
BpmI	CTGGAG	2 cut(s)	1065, 1750
Bpu10I	CCTNAGC	2 cut(s)	1294, 2069
Bpu14I	TTCGAA	1 cut(s)	641
BpuEI	CTTGAG	1 cut(s)	2384
BpuMI	CCSGG	7 cut(s)	905, 936, 1743, 1744, 1773, 1801, 1922
Bsa29I	ATCGAT	1 cut(s)	513
BsaAI	YACGTR	1 cut(s)	2179
BsaHI	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
BsaI	GGTCTC	2 cut(s)	1405, 1603
BsaWI	WCCGGW	1 cut(s)	837
BsaXI	ACNNNNNCTCC	2 cut(s)	565, 595
Bse118I	RCCGGY	3 cut(s)	233, 295, 2094
Bse1I	ACTGG	4 cut(s)	287, 330, 875, 1970
Bse3DI	GCAATG	1 cut(s)	58
BseBI	CCWGG	4 cut(s)	942, 1673, 1760, 2312
BseCI	ATCGAT	1 cut(s)	513
BseGI	GGATG	5 cut(s)	732, 756, 1202, 1755, 2358
BseMI	GCAATG	1 cut(s)	58
BseMII	CTCAG	4 cut(s)	1308, 1324, 2060, 2345
BseNI	ACTGG	4 cut(s)	287, 330, 875, 1970
BseSI	GKGCMC	3 cut(s)	941, 976, 1965
BseX3I	CGGCCG	2 cut(s)	585, 2447
BseYI	CCCAGC	4 cut(s)	501, 762, 806, 1479
BsgI	GTGCAG	1 cut(s)	1227
Bsh1236I	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
Bsh1285I	CGRYCG	2 cut(s)	588, 2450
BshNI	GGYRCC	3 cut(s)	971, 1469, 2035
BshVI	ATCGAT	1 cut(s)	513
BsiEI	CGRYCG	2 cut(s)	588, 2450
BsiHKAI	GWGCWC	3 cut(s)	16, 1455, 1965
BsiHKCI	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
BslFI	GGGAC	6 cut(s)	863, 1063, 1091, 1556, 1822, 2407
BsmAI	GTCTC	6 cut(s)	757, 913, 1306, 1341, 1405, 1603
BsmBI	CGTCTC	2 cut(s)	757, 1341
BsmFI	GGGAC	6 cut(s)	863, 1063, 1091, 1556, 1822, 2407
BsmI	GAATGC	1 cut(s)	1975
Bso31I	GGTCTC	2 cut(s)	1405, 1603
BsoBI	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
Bsp119I	TTCGAA	1 cut(s)	641
Bsp120I	GGGCCC	1 cut(s)	937
Bsp1286I	GDGCHC	8 cut(s)	16, 941, 949, 976, 1455, 1806, 1965, 2084
Bsp143I	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
Bsp19I	CCATGG	1 cut(s)	675
BspCNI	CTCAG	4 cut(s)	1307, 1323, 2061, 2344
BspDI	ATCGAT	1 cut(s)	513
BspFNI	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
BspMAI	CTGCAG	2 cut(s)	490, 1974
BspMI	ACCTGC	2 cut(s)	451, 1124
BspPI	GGATC	4 cut(s)	509, 725, 1663, 2424
BspT104I	TTCGAA	1 cut(s)	641
BspT107I	GGYRCC	3 cut(s)	971, 1469, 2035
BspTI	CTTAAG	1 cut(s)	950
BspTNI	GGTCTC	2 cut(s)	1405, 1603
BsrBI	CCGCTC	2 cut(s)	2247, 2292
BsrDI	GCAATG	1 cut(s)	58
BsrFI	RCCGGY	3 cut(s)	233, 295, 2094
BsrI	ACTGG	4 cut(s)	287, 330, 875, 1970
BssAI	RCCGGY	3 cut(s)	233, 295, 2094
BssMI	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
BssNI	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
BssT1I	CCWWGG	3 cut(s)	675, 1824, 2139
Bst2UI	CCWGG	4 cut(s)	942, 1673, 1760, 2312
Bst4CI	ACNGT	1 cut(s)	2053
Bst6I	CTCTTC	4 cut(s)	876, 1213, 1320, 2154
BstACI	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
BstAFI	CTTAAG	1 cut(s)	950
BstAPI	GCANNNNNTGC	2 cut(s)	1969, 2194
BstBAI	YACGTR	1 cut(s)	2179
BstBI	TTCGAA	1 cut(s)	641
BstC8I	GCNNGC	6 cut(s)	496, 1519, 2080, 2149, 2234, 2426
BstDEI	CTNAG	4 cut(s)	1294, 1310, 2069, 2331
BstDSI	CCRYGG	6 cut(s)	82, 262, 675, 699, 1833, 1893
BstF5I	GGATG	5 cut(s)	732, 756, 1202, 1755, 2358
BstFNI	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
BstH2I	RGCGCY	2 cut(s)	821, 2039
BstHHI	GCGC	5 cut(s)	186, 820, 1391, 1485, 2038
BstKTI	GATC	8 cut(s)	201, 517, 573, 720, 957, 1671, 1714, 2419
BstMAI	GTCTC	6 cut(s)	757, 913, 1306, 1341, 1405, 1603
BstMBI	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
BstMCI	CGRYCG	2 cut(s)	588, 2450
BstNI	CCWGG	4 cut(s)	942, 1673, 1760, 2312
BstPAI	GACNNNNGTC	1 cut(s)	838
BstSFI	CTRYAG	3 cut(s)	486, 1970, 2152
BstSLI	GKGCMC	3 cut(s)	941, 976, 1965
BstUI	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
BstV2I	GAAGAC	2 cut(s)	687, 1606
BstX2I	RGATCY	1 cut(s)	2416
BstYI	RGATCY	1 cut(s)	2416
BstZI	CGGCCG	2 cut(s)	585, 2447
Bsu15I	ATCGAT	1 cut(s)	513
BsuTUI	ATCGAT	1 cut(s)	513
BtgI	CCRYGG	6 cut(s)	82, 262, 675, 699, 1833, 1893
BtgZI	GCGATG	1 cut(s)	515
BtsCI	GGATG	5 cut(s)	732, 756, 1202, 1755, 2358
BtsI	GCAGTG	1 cut(s)	2232
BtsIMutI	CAGTG	2 cut(s)	1963, 2232
BveI	ACCTGC	2 cut(s)	451, 1124
Cac8I	GCNNGC	6 cut(s)	496, 1519, 2080, 2149, 2234, 2426
CaiI	CAGNNNCTG	3 cut(s)	494, 1533, 2194
CciNI	GCGGCCGC	1 cut(s)	2447
CfoI	GCGC	5 cut(s)	186, 820, 1391, 1485, 2038
Cfr10I	RCCGGY	3 cut(s)	233, 295, 2094
Cfr42I	CCGCGG	1 cut(s)	265
Cfr9I	CCCGGG	1 cut(s)	1742
ClaI	ATCGAT	1 cut(s)	513
CseI	GACGC	3 cut(s)	139, 180, 1474
Csp6I	GTAC	4 cut(s)	386, 829, 1027, 2371
CviAII	CATG	8 cut(s)	187, 676, 705, 910, 980, 1510, 2442, 2459
CviQI	GTAC	4 cut(s)	386, 829, 1027, 2371
DdeI	CTNAG	4 cut(s)	1294, 1310, 2069, 2331
DinI	GGCGCC	1 cut(s)	2037
DpnI	GATC	8 cut(s)	200, 516, 572, 719, 956, 1670, 1713, 2418
DpnII	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
DraIII	CACNNNGTG	1 cut(s)	2182
DrdI	GACNNNNNNGTC	2 cut(s)	559, 1776
DseDI	GACNNNNNNGTC	2 cut(s)	559, 1776
EaeI	YGGCCR	2 cut(s)	585, 2447
EagI	CGGCCG	2 cut(s)	585, 2447
Eam1104I	CTCTTC	4 cut(s)	876, 1213, 1320, 2154
EarI	CTCTTC	4 cut(s)	876, 1213, 1320, 2154
EciI	GGCGGA	9 cut(s)	273, 661, 1025, 1068, 1077, 1235, 1465, 1591, 1913
Ecl136II	GAGCTC	1 cut(s)	1453
EclXI	CGGCCG	2 cut(s)	585, 2447
Eco130I	CCWWGG	3 cut(s)	675, 1824, 2139
Eco147I	AGGCCT	2 cut(s)	1359, 1823
Eco24I	GRGCYC	5 cut(s)	941, 949, 1455, 1806, 2084
Eco31I	GGTCTC	2 cut(s)	1405, 1603
Eco32I	GATATC	1 cut(s)	1990
Eco47I	GGWCC	8 cut(s)	506, 727, 840, 932, 1699, 1778, 1809, 1918
Eco52I	CGGCCG	2 cut(s)	585, 2447
Eco53kI	GAGCTC	1 cut(s)	1453
Eco72I	CACGTG	1 cut(s)	2179
Eco88I	CYCGRG	5 cut(s)	824, 880, 1742, 1805, 2348
EcoICRI	GAGCTC	1 cut(s)	1453
EcoO109I	RGGNCCY	5 cut(s)	506, 727, 938, 1729, 1809
EcoRII	CCWGG	4 cut(s)	940, 1671, 1758, 2310
EcoRV	GATATC	1 cut(s)	1990
EcoT14I	CCWWGG	3 cut(s)	675, 1824, 2139
EcoT38I	GRGCYC	5 cut(s)	941, 949, 1455, 1806, 2084
EgeI	GGCGCC	1 cut(s)	2037
EheI	GGCGCC	1 cut(s)	2037
ErhI	CCWWGG	3 cut(s)	675, 1824, 2139
Esp3I	CGTCTC	2 cut(s)	757, 1341
FaeI	CATG	8 cut(s)	190, 679, 708, 913, 983, 1513, 2445, 2462
FalI	AAGNNNNNCTT	2 cut(s)	91, 123
FaqI	GGGAC	6 cut(s)	863, 1063, 1091, 1556, 1822, 2407
FatI	CATG	8 cut(s)	186, 675, 704, 909, 979, 1509, 2441, 2458
FauI	CCCGC	9 cut(s)	257, 266, 1255, 1284, 1526, 2071, 2225, 2285, 2308
FokI	GGATG	5 cut(s)	719, 743, 1189, 1742, 2365
FriOI	GRGCYC	5 cut(s)	941, 949, 1455, 1806, 2084
FspBI	CTAG	2 cut(s)	1868, 2202
GlaI	GCGC	5 cut(s)	185, 819, 1390, 1484, 2037
GsaI	CCCAGC	4 cut(s)	505, 766, 810, 1483
GsuI	CTGGAG	2 cut(s)	1065, 1750
HaeII	RGCGCY	2 cut(s)	821, 2039
HgaI	GACGC	3 cut(s)	139, 180, 1474
HhaI	GCGC	5 cut(s)	186, 820, 1391, 1485, 2038
Hin1I	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
Hin1II	CATG	8 cut(s)	190, 679, 708, 913, 983, 1513, 2445, 2462
Hin6I	GCGC	5 cut(s)	184, 818, 1389, 1483, 2036
HinP1I	GCGC	5 cut(s)	184, 818, 1389, 1483, 2036
HindIII	AAGCTT	1 cut(s)	2402
Hpy166II	GTNNAC	5 cut(s)	564, 1227, 1702, 1963, 2373
Hpy188III	TCNNGA	8 cut(s)	880, 962, 1044, 1715, 1767, 1807, 2202, 2350
Hpy8I	GTNNAC	5 cut(s)	564, 1227, 1702, 1963, 2373
Hpy99I	CGWCG	8 cut(s)	155, 562, 989, 1421, 1490, 1712, 2242, 2369
HpyCH4III	ACNGT	1 cut(s)	2053
HpyCH4IV	ACGT	7 cut(s)	416, 560, 750, 984, 1788, 2178, 2217
HpyCH4V	TGCA	9 cut(s)	488, 801, 1033, 1119, 1244, 1726, 1963, 1972, 2026
HpyF3I	CTNAG	4 cut(s)	1294, 1310, 2069, 2331
HpySE526I	ACGT	7 cut(s)	416, 560, 750, 984, 1788, 2178, 2217
Hsp92I	GRCGYC	5 cut(s)	191, 416, 560, 984, 2036
Hsp92II	CATG	8 cut(s)	190, 679, 708, 913, 983, 1513, 2445, 2462
HspAI	GCGC	5 cut(s)	184, 818, 1389, 1483, 2036
KasI	GGCGCC	1 cut(s)	2035
KspI	CCGCGG	1 cut(s)	265
Kzo9I	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
LmnI	GCTCC	9 cut(s)	692, 746, 825, 1440, 1535, 2059, 2144, 2185, 2244
LweI	GCATC	3 cut(s)	1320, 1551, 1632
MaeI	CTAG	2 cut(s)	1868, 2202
MaeII	ACGT	7 cut(s)	416, 560, 750, 984, 1788, 2178, 2217
MaeIII	GTNAC	5 cut(s)	23, 378, 438, 2047, 2213
MalI	GATC	8 cut(s)	200, 516, 572, 719, 956, 1670, 1713, 2418
MbiI	CCGCTC	2 cut(s)	2247, 2292
MboI	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
MfeI	CAATTG	1 cut(s)	2266
MflI	RGATCY	1 cut(s)	2416
MhlI	GDGCHC	8 cut(s)	16, 941, 949, 976, 1455, 1806, 1965, 2084
MluCI	AATT	5 cut(s)	133, 274, 400, 1443, 2266
Mly113I	GGCGCC	1 cut(s)	2036
MlyI	GAGTC	5 cut(s)	625, 828, 959, 1232, 2389
MmeI	TCCRAC	4 cut(s)	162, 917, 2031, 2388
MroXI	GAANNNNTTC	1 cut(s)	103
MseI	TTAA	4 cut(s)	273, 888, 951, 1155
MslI	CAYNNNNRTG	2 cut(s)	1430, 2378
MspA1I	CMGCKG	9 cut(s)	48, 264, 422, 485, 494, 501, 656, 1241, 2194
MspCI	CTTAAG	1 cut(s)	950
MunI	CAATTG	1 cut(s)	2266
Mva1269I	GAATGC	1 cut(s)	1975
MvaI	CCWGG	4 cut(s)	942, 1673, 1760, 2312
MvnI	CGCG	6 cut(s)	264, 815, 1391, 1485, 1490, 2078
NarI	GGCGCC	1 cut(s)	2036
NciI	CCSGG	7 cut(s)	905, 936, 1743, 1744, 1773, 1801, 1922
NcoI	CCATGG	1 cut(s)	675
NdeII	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
NlaIII	CATG	8 cut(s)	190, 679, 708, 913, 983, 1513, 2445, 2462
NmeAIII	GCCGAG	1 cut(s)	1518
NmuCI	GTSAC	2 cut(s)	23, 438
NotI	GCGGCCGC	1 cut(s)	2447
NspV	TTCGAA	1 cut(s)	641
OliI	CACNNNNGTG	1 cut(s)	2378
PaeR7I	CTCGAG	1 cut(s)	824
PaqCI	CACCTGC	1 cut(s)	451
PasI	CCCWGGG	1 cut(s)	941
PceI	AGGCCT	2 cut(s)	1359, 1823
PcsI	WCGNNNNNNNCGW	3 cut(s)	548, 1446, 1713
PctI	GAATGC	1 cut(s)	1975
PdmI	GAANNNNTTC	1 cut(s)	103
PfeI	GAWTC	5 cut(s)	544, 643, 2003, 2135, 2209
PflFI	GACNNNGTC	2 cut(s)	29, 1697
PflMI	CCANNNNNTGG	3 cut(s)	705, 1594, 1759
PfoI	TCCNGGA	2 cut(s)	903, 1671
PleI	GAGTC	5 cut(s)	625, 828, 959, 1231, 2389
PluTI	GGCGCC	1 cut(s)	2039
PmaCI	CACGTG	1 cut(s)	2179
PmlI	CACGTG	1 cut(s)	2179
PpsI	GAGTC	5 cut(s)	625, 828, 959, 1231, 2389
Ppu21I	YACGTR	1 cut(s)	2179
PpuMI	RGGWCCY	3 cut(s)	506, 727, 1809
PshAI	GACNNNNGTC	1 cut(s)	838
PshBI	ATTAAT	1 cut(s)	273
Psp124BI	GAGCTC	1 cut(s)	1455
Psp1406I	AACGTT	1 cut(s)	2217
Psp5II	RGGWCCY	3 cut(s)	506, 727, 1809
Psp6I	CCWGG	4 cut(s)	940, 1671, 1758, 2310
PspCI	CACGTG	1 cut(s)	2179
PspFI	CCCAGC	4 cut(s)	501, 762, 806, 1479
PspGI	CCWGG	4 cut(s)	940, 1671, 1758, 2310
PspOMI	GGGCCC	1 cut(s)	937
PspPPI	RGGWCCY	3 cut(s)	506, 727, 1809
PspXI	VCTCGAGB	1 cut(s)	824
PsrI	GAACNNNNNNTAC	1 cut(s)	2468
PstI	CTGCAG	2 cut(s)	490, 1974
PstNI	CAGNNNCTG	3 cut(s)	494, 1533, 2194
PsuI	RGATCY	1 cut(s)	2416
PsyI	GACNNNGTC	2 cut(s)	29, 1697
PvuII	CAGCTG	4 cut(s)	422, 494, 501, 2194
RsaI	GTAC	4 cut(s)	387, 830, 1028, 2372
RsaNI	GTAC	4 cut(s)	386, 829, 1027, 2371
RseI	CAYNNNNRTG	2 cut(s)	1430, 2378
SacI	GAGCTC	1 cut(s)	1455
SacII	CCGCGG	1 cut(s)	265
SaqAI	TTAA	4 cut(s)	273, 888, 951, 1155
Sau3AI	GATC	8 cut(s)	198, 514, 570, 717, 954, 1668, 1711, 2416
SchI	GAGTC	5 cut(s)	625, 828, 959, 1232, 2389
SduI	GDGCHC	8 cut(s)	16, 941, 949, 976, 1455, 1806, 1965, 2084
SfaNI	GCATC	3 cut(s)	1320, 1551, 1632
SfcI	CTRYAG	3 cut(s)	486, 1970, 2152
SfiI	GGCCNNNNNGGCC	1 cut(s)	1829
SfoI	GGCGCC	1 cut(s)	2037
Sfr274I	CTCGAG	1 cut(s)	824
Sfr303I	CCGCGG	1 cut(s)	265
SfuI	TTCGAA	1 cut(s)	641
SgrBI	CCGCGG	1 cut(s)	265
SinI	GGWCC	8 cut(s)	506, 727, 840, 932, 1699, 1778, 1809, 1918
SlaI	CTCGAG	1 cut(s)	824
SmaI	CCCGGG	1 cut(s)	1744
SmiMI	CAYNNNNRTG	2 cut(s)	1430, 2378
SmlI	CTYRAG	3 cut(s)	824, 950, 2399
SmoI	CTYRAG	3 cut(s)	824, 950, 2399
SrfI	GCCCGGGC	1 cut(s)	1744
Sse9I	AATT	5 cut(s)	133, 274, 400, 1443, 2266
SseBI	AGGCCT	2 cut(s)	1359, 1823
SspDI	GGCGCC	1 cut(s)	2035
SspMI	CTAG	2 cut(s)	1868, 2202
SstI	GAGCTC	1 cut(s)	1455
StuI	AGGCCT	2 cut(s)	1359, 1823
StyI	CCWWGG	3 cut(s)	675, 1824, 2139
TaaI	ACNGT	1 cut(s)	2053
TaiI	ACGT	7 cut(s)	419, 563, 753, 987, 1791, 2181, 2220
TasI	AATT	5 cut(s)	133, 274, 400, 1443, 2266
TatI	WGTACW	2 cut(s)	828, 1026
TfiI	GAWTC	5 cut(s)	544, 643, 2003, 2135, 2209
Tru1I	TTAA	4 cut(s)	273, 888, 951, 1155
Tru9I	TTAA	4 cut(s)	273, 888, 951, 1155
TscAI	CASTG	2 cut(s)	1970, 2232
TseFI	GTSAC	2 cut(s)	23, 438
Tsp45I	GTSAC	2 cut(s)	23, 438
TspDTI	ATGAA	3 cut(s)	131, 518, 2447
TspGWI	ACGGA	2 cut(s)	1558, 1866
TspMI	CCCGGG	1 cut(s)	1742
TspRI	CASTG	2 cut(s)	1970, 2232
Tth111I	GACNNNGTC	2 cut(s)	29, 1697
Van91I	CCANNNNNTGG	3 cut(s)	705, 1594, 1759
Vha464I	CTTAAG	1 cut(s)	950
VneI	GTGCAC	1 cut(s)	1961
VpaK11BI	GGWCC	8 cut(s)	506, 727, 840, 932, 1699, 1778, 1809, 1918
VspI	ATTAAT	1 cut(s)	273
XapI	RAATTY	2 cut(s)	400, 1443
XbaI	TCTAGA	1 cut(s)	2201
XcmI	CCANNNNNNNNNTGG	1 cut(s)	89
XhoI	CTCGAG	1 cut(s)	824
XmaI	CCCGGG	1 cut(s)	1742
XmnI	GAANNNNTTC	1 cut(s)	103
XspI	CTAG	2 cut(s)	1868, 2202
ZraI	GACGTC	3 cut(s)	417, 561, 985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.