Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0014426

Physical Location & Seq

Genomic Coordinates Forward (+)

20448199 .. 20451407

Gene Structure 3209 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g18250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGGCTGCTTGTGCTCTCCTCGGTGACAATGGTGGCGATCGCATCAGCGGCAATGGCAGTAGTAACAACAACAATAGCGATAGCCTCGGCGGCGGTGGAGGAAACCCTTCTTACCCCATGAACAACAGCACTACCAATTCCAACAGCAGCAGCGTCGAGCAGCAGCCACCCAAGATGGTGAGAAAGCGAATGGCGTCTGAGATCGAAGTCCAAACCACCCTGACAAGCAGAACCGCCGGTTCGGACTATTTCAGGCTTTCCCGCCGCGGGATTATTAATAATAGTCCGCCAGTCCAGCCGGTTAGTCAAAATCCAAACGCCAGTAAAGTGAATAGTAGTATGTTTTATCCCAACCACTCCACTATGCTGTTACCAGTACCATCTTCCACAAATTTGGCCGCTTTGACGTCACCTGGTGGGGCTTTATTGTCACCTACTTCTCCTTCTCCTTCTCCTTCTTCCGCTGCAGCAGCCGGCACCTGGGGTCCTATCGATCCATTGTCTTTTCATCGTCCTCCTCCGGTTCAGCCGACGACGACCACCCAGACCACTACTACTCCTGCCGTTTGTGGGTTTTCAGGCCTGCCGTTGTTTCCGCCGGAAAAGACTACTCCTTTGAATACTAGTATTACTGCTACTACTCCGTCGTCCGTGTCCATGGAAGACGGGTCCTCCGCCGCCACGGCGTGGATAGACGGGATCATAAAGGACCTCGTCCACAGCTCCACCAACGTCTCCATCCCCCAGCTCATTCATAATGTTAGGGAGATTATCTTCCCTTGCAACCCCAGCCTCGCCGCTCTTCTGGAGTATAGACTCCGGTCAATATCCGAGCCGCTGCCGCCGCCTCCTCTGCCTCTTCCAGTTCCAAGCTTTAATCCAACCCCAATCCCCGACATGAGGAGGAGACAACAAGGTGGTCCTGGCCCTGGGGCTCTTAAGCTCAATCTGGACTCTGGTGCAATACATGACGTTGCAATCTTCAACCCATCAACGGTGGAAAACAATGACTTGTACTTGCAGTCGTGGTCTGGAGGAGCTGGCGGAGGAGGAGTTGGGCTGTCCCTACCCCAACCCAACCCTACTGCTGCCCCTATCACCTGCAACCAAACCAACCCACATCACCAAAGCCCTTCCTTCAATCAGGCAATTCATCAAACACAAGACAAGCAGTTGGAGAACTCTTCATCATCCTCCCCTGCGGCGGAGTCCACCACCCCAACAGCTGCACCCGCACCCACAACCACTGCCGCCACTGCCCCGCCAGAACCAACAACCTCAGCAGTATCCCTACTCAGAGAGAAAAAAGAAGAGATACGGCAGCAAAAGCGAGACGAGGAAGGCCTCCACCTCCTCACCCTCCTCCTCAAATGCGCGGAGGCCGTCTCCGCCGACAACTTCGACGAAGCCACAAAAATCCTCCTCGAAATCTCGGAGCTCTCCACCCCGTTCGGCACCTCCGCCCAGCGCGTCGCGGCCTACTTCTCTGAAGCCATGTCGGCCCGCCTCGTCAGCTCCTGCCTAGGCATCTACGCCTCCCTCCCTCGCTCCTCCGTCCCCACCACCCACACACAAAAAATGGTCTCCGCCTTCCAAGTCTTCAACGGCATCAGCCCCTTCATCAAGTTCTCCCACTTCACAGCCAATCAGGCGATCCAGGAGGCGTTCGAGCGGGAGGACAGGGTTCACATCGTCGATCTCGACATAATGCAGGGTCTCCAATGGCCCGGGCTGTTTCACATCCTGGCCTCCAGGCCTGGTGGGCCTCCGTACGTGAGACTCACCGGCCTCGGAACCTCAATGGAGGCCTTGGAGGCCACAGGGAAGCGGTTGTCCGACTTCGCGGACAAGCTAGGGCTTCCATTTGAGTTCTTCCCCGTGGTGGAGAAAGTTGCGAACTTGGACCCGGAGAGGCTGAATATCAGCAAGAGAGAGGCTGTGGCGGTGCATTGGCTTCAGCATTCGCTTTACGACGTCACGGGTTCCGATTCCAGCACGCTCTGGCTTTTGCAGAGATTGGCGCCAAAAGTGGTAACGGTGGTGGAGCAAGACCTGAGCCATGCGGGGTCGTTCTTGGGCCGGTTTGTGGAGGCCATACACTACTACTCTGCCCTGTTTGATTCCTTGGGAGCGAGCTACGGCGAGGAGAGCGAGGAGAGGCACGTGGTGGAGCAGCAGCTGCTCTCCAGAGAGATTCGTAACGTTTTGGCAGTGGGTGGGCCGTCGAGGAGCGGAGAGGTGAAGTTCCACAACTGGAGGGAGAAGTTTCAGCAGACCGGGTTCAGGGGGATTTCCCTGGCGGGAAATGCTGCAAATCAGGCCTCCTTGCTGCTCGGGATGTTCCCTTCCGATGGGTACACTCTGGTGGAGGACAATGGGACTCTTAAACTGGGGTGGAAGGATCTCTGCTTGCTCACCGCTTCTGCATGGCGGCCGCCGTTTCATGCCGCCGCTAATCCAAACCTACACTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008356 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009889 GO:0009956 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032507 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045165 GO:0045185 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051235 GO:0051252 GO:0051301 GO:0051457 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0072595 GO:0080090 GO:0090610 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

825

Amino Acids

88.16

Weight (kDa)

5.98

Isoelectric Point (pI)

56.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	452 - 811	1e-130	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014426)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54220
fragaria_vesca	FvH4_6g09040
malus_domestica	MD04G1181500.v1.1 MD12G1196300.v1.1
prunus_persica	Prupe.6G300900_v2.0.a1
pyrus_communis	pycom04g16040 pycom12g18250
rosa_chinensis	RchiOBHm_Chr3g0459291
rosa_laevigata	RLG00000025096
rosa_multiflora	Rmu_sc0012999.1_g000002
rosa_roxburghii	Rroxscaffold_6G00420330
rosa_rugosa	Rorug03G0035100
rosa_samantha	Rh3AG092900 Rh3BG096000 Rh3CG097000 Rh3DG096600
rosa_wichuraiana	Rw3G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1109
AatII	GACGTC	2 cut(s)	410, 1977
Acc36I	ACCTGC	1 cut(s)	1109
AccB1I	GGYRCC	3 cut(s)	476, 1454, 2020
AccB7I	CCANNNNNTGG	1 cut(s)	687
AccBSI	CCGCTC	3 cut(s)	800, 1672, 2232
AccII	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
AclI	AACGTT	1 cut(s)	2202
AclWI	GGATC	4 cut(s)	488, 707, 1648, 2409
AcoI	YGGCCR	2 cut(s)	396, 2432
AcsI	RAATTY	1 cut(s)	391
AcuI	CTGAAG	2 cut(s)	1509, 1940
AcvI	CACGTG	1 cut(s)	2164
AcyI	GRCGYC	4 cut(s)	194, 407, 1974, 2021
AdeI	CACNNNGTG	3 cut(s)	416, 687, 2167
AfaI	GTAC	4 cut(s)	378, 1016, 1772, 2357
AflII	CTTAAG	1 cut(s)	938
AgsI	TTSAA	4 cut(s)	619, 985, 1141, 1603
AhlI	ACTAGT	1 cut(s)	623
AjnI	CCWGG	9 cut(s)	412, 479, 922, 928, 1656, 1743, 1751, 1756, 2295
AleI	CACNNNNGTG	1 cut(s)	2363
Alw21I	GWGCWC	2 cut(s)	16, 1440
Alw26I	GTCTC	7 cut(s)	739, 901, 1326, 1390, 1588, 1721, 1771
AlwI	GGATC	4 cut(s)	488, 707, 1648, 2409
AlwNI	CAGNNNCTG	2 cut(s)	1518, 2179
Ama87I	CYCGRG	2 cut(s)	1727, 2333
ApoI	RAATTY	1 cut(s)	391
ArsI	GACNNNNNNTTYG	2 cut(s)	598, 630
AseI	ATTAAT	1 cut(s)	276
AsiSI	GCGATCGC	1 cut(s)	40
Asp700I	GAANNNNTTC	1 cut(s)	106
AspA2I	CCTAGG	1 cut(s)	1522
AspLEI	GCGC	3 cut(s)	1376, 1470, 2023
AsuC2I	CCSGG	4 cut(s)	1728, 1729, 1907, 2278
AvaI	CYCGRG	2 cut(s)	1727, 2333
AvaII	GGWCC	5 cut(s)	485, 669, 709, 920, 1903
AvrII	CCTAGG	1 cut(s)	1522
BanI	GGYRCC	3 cut(s)	476, 1454, 2020
BanII	GRGCYC	2 cut(s)	937, 1440
BbrPI	CACGTG	1 cut(s)	2164
BbsI	GAAGAC	2 cut(s)	669, 1591
Bbv12I	GWGCWC	2 cut(s)	16, 1440
BbvCI	CCTCAGC	1 cut(s)	1279
BccI	CCATC	5 cut(s)	169, 388, 746, 997, 2345
BceAI	ACGGC	9 cut(s)	548, 571, 699, 1334, 1367, 1621, 2155, 2206, 2422
BcgI	CGANNNNNNTGC	2 cut(s)	136, 170
BciT130I	CCWGG	9 cut(s)	414, 481, 924, 930, 1658, 1745, 1753, 1758, 2297
BciVI	GTATCC	1 cut(s)	1297
BcnI	CCSGG	4 cut(s)	1728, 1729, 1907, 2278
BcoDI	GTCTC	7 cut(s)	739, 901, 1326, 1390, 1588, 1721, 1771
BcuI	ACTAGT	1 cut(s)	623
BfaI	CTAG	3 cut(s)	624, 1523, 1853
BfmI	CTRYAG	1 cut(s)	465
BfoI	RGCGCY	1 cut(s)	2024
BfrI	CTTAAG	1 cut(s)	938
BfuAI	ACCTGC	1 cut(s)	1109
BfuI	GTATCC	1 cut(s)	1297
BglI	GCCNNNNNGGC	6 cut(s)	90, 683, 1499, 1649, 1762, 1814
BlnI	CCTAGG	1 cut(s)	1522
Bme18I	GGWCC	5 cut(s)	485, 669, 709, 920, 1903
BmeT110I	CYCGRG	2 cut(s)	1727, 2333
BmiI	GGNNCC	8 cut(s)	478, 486, 670, 1456, 1795, 1905, 1984, 2022
BmrI	ACTGGG	1 cut(s)	2399
BmsI	GCATC	3 cut(s)	51, 1536, 1617
BmuI	ACTGGG	1 cut(s)	2399
BoxI	GACNNNNGTC	1 cut(s)	820
BpiI	GAAGAC	2 cut(s)	669, 1591
BplI	GAGNNNNNCTC	2 cut(s)	1370, 1402
BpmI	CTGGAG	5 cut(s)	827, 1053, 1735, 2170, 2275
Bpu10I	CCTNAGC	2 cut(s)	1279, 2054
BpuMI	CCSGG	4 cut(s)	1728, 1729, 1907, 2278
Bsa29I	ATCGAT	1 cut(s)	492
BsaAI	YACGTR	2 cut(s)	1774, 2164
BsaHI	GRCGYC	4 cut(s)	194, 407, 1974, 2021
BsaI	GGTCTC	2 cut(s)	1588, 1721
BsaWI	WCCGGW	2 cut(s)	520, 819
BsaXI	ACNNNNNCTCC	6 cut(s)	541, 571, 656, 686, 1038, 1068
Bse118I	RCCGGY	5 cut(s)	236, 298, 473, 1784, 2079
Bse1I	ACTGG	6 cut(s)	290, 321, 374, 863, 2258, 2394
Bse3DI	GCAATG	1 cut(s)	58
BseBI	CCWGG	9 cut(s)	414, 481, 924, 930, 1658, 1745, 1753, 1758, 2297
BseCI	ATCGAT	1 cut(s)	492
BseGI	GGATG	4 cut(s)	738, 1190, 1740, 2343
BseMI	GCAATG	1 cut(s)	58
BseMII	CTCAG	4 cut(s)	189, 1293, 1309, 2045
BseNI	ACTGG	6 cut(s)	290, 321, 374, 863, 2258, 2394
BseX3I	CGGCCG	1 cut(s)	2432
BseYI	CCCAGC	3 cut(s)	744, 788, 1464
BsgI	GTGCAG	1 cut(s)	1212
Bsh1236I	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
Bsh1285I	CGRYCG	2 cut(s)	40, 2435
BshNI	GGYRCC	3 cut(s)	476, 1454, 2020
BshVI	ATCGAT	1 cut(s)	492
BsiEI	CGRYCG	2 cut(s)	40, 2435
BsiHKAI	GWGCWC	2 cut(s)	16, 1440
BsiHKCI	CYCGRG	2 cut(s)	1727, 2333
BsiWI	CGTACG	1 cut(s)	1770
BslFI	GGGAC	3 cut(s)	1048, 1541, 2392
BsmAI	GTCTC	7 cut(s)	739, 901, 1326, 1390, 1588, 1721, 1771
BsmBI	CGTCTC	3 cut(s)	739, 1326, 1390
BsmFI	GGGAC	3 cut(s)	1048, 1541, 2392
BsmI	GAATGC	1 cut(s)	1960
Bso31I	GGTCTC	2 cut(s)	1588, 1721
BsoBI	CYCGRG	2 cut(s)	1727, 2333
Bsp1286I	GDGCHC	3 cut(s)	16, 937, 1440
Bsp143I	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
Bsp19I	CCATGG	1 cut(s)	657
BspCNI	CTCAG	4 cut(s)	190, 1292, 1308, 2046
BspDI	ATCGAT	1 cut(s)	492
BspFNI	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
BspLI	GGNNCC	8 cut(s)	478, 486, 670, 1456, 1795, 1905, 1984, 2022
BspMAI	CTGCAG	1 cut(s)	469
BspMI	ACCTGC	1 cut(s)	1109
BspPI	GGATC	4 cut(s)	488, 707, 1648, 2409
BspQI	GCTCTTC	1 cut(s)	807
BspT107I	GGYRCC	3 cut(s)	476, 1454, 2020
BspTI	CTTAAG	1 cut(s)	938
BspTNI	GGTCTC	2 cut(s)	1588, 1721
BsrBI	CCGCTC	3 cut(s)	800, 1672, 2232
BsrDI	GCAATG	1 cut(s)	58
BsrFI	RCCGGY	5 cut(s)	236, 298, 473, 1784, 2079
BsrI	ACTGG	6 cut(s)	290, 321, 374, 863, 2258, 2394
BssAI	RCCGGY	5 cut(s)	236, 298, 473, 1784, 2079
BssMI	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
BssNI	GRCGYC	4 cut(s)	194, 407, 1974, 2021
BssT1I	CCWWGG	4 cut(s)	657, 1522, 1809, 2124
Bst2UI	CCWGG	9 cut(s)	414, 481, 924, 930, 1658, 1745, 1753, 1758, 2297
Bst4CI	ACNGT	2 cut(s)	997, 2038
Bst6I	CTCTTC	4 cut(s)	807, 864, 1189, 1305
BstACI	GRCGYC	4 cut(s)	194, 407, 1974, 2021
BstAFI	CTTAAG	1 cut(s)	938
BstAPI	GCANNNNNTGC	1 cut(s)	2179
BstBAI	YACGTR	2 cut(s)	1774, 2164
BstC8I	GCNNGC	7 cut(s)	475, 584, 1042, 1504, 1997, 2134, 2411
BstDEI	CTNAG	4 cut(s)	198, 1279, 1295, 2054
BstDSI	CCRYGG	4 cut(s)	265, 657, 681, 1878
BstF5I	GGATG	4 cut(s)	738, 1190, 1740, 2343
BstFNI	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
BstH2I	RGCGCY	1 cut(s)	2024
BstHHI	GCGC	3 cut(s)	1376, 1470, 2023
BstKTI	GATC	7 cut(s)	40, 204, 496, 702, 1656, 1699, 2404
BstMAI	GTCTC	7 cut(s)	739, 901, 1326, 1390, 1588, 1721, 1771
BstMBI	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
BstMCI	CGRYCG	2 cut(s)	40, 2435
BstNI	CCWGG	9 cut(s)	414, 481, 924, 930, 1658, 1745, 1753, 1758, 2297
BstPAI	GACNNNNGTC	1 cut(s)	820
BstSFI	CTRYAG	1 cut(s)	465
BstUI	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
BstV2I	GAAGAC	2 cut(s)	669, 1591
BstX2I	RGATCY	1 cut(s)	2401
BstXI	CCANNNNNNTGG	1 cut(s)	394
BstYI	RGATCY	1 cut(s)	2401
BstZI	CGGCCG	1 cut(s)	2432
Bsu15I	ATCGAT	1 cut(s)	492
BsuI	GTATCC	1 cut(s)	1297
BsuTUI	ATCGAT	1 cut(s)	492
BtgI	CCRYGG	4 cut(s)	265, 657, 681, 1878
BtsCI	GGATG	4 cut(s)	738, 1190, 1740, 2343
BtsI	GCAGTG	3 cut(s)	1245, 1254, 2217
BtsIMutI	CAGTG	3 cut(s)	1245, 1254, 2217
BveI	ACCTGC	1 cut(s)	1109
Cac8I	GCNNGC	7 cut(s)	475, 584, 1042, 1504, 1997, 2134, 2411
CaiI	CAGNNNCTG	2 cut(s)	1518, 2179
CciNI	GCGGCCGC	1 cut(s)	2432
CfoI	GCGC	3 cut(s)	1376, 1470, 2023
Cfr10I	RCCGGY	5 cut(s)	236, 298, 473, 1784, 2079
Cfr42I	CCGCGG	1 cut(s)	268
Cfr9I	CCCGGG	1 cut(s)	1727
ClaI	ATCGAT	1 cut(s)	492
CseI	GACGC	3 cut(s)	142, 183, 1459
CsiI	ACCWGGT	1 cut(s)	412
Csp6I	GTAC	4 cut(s)	377, 1015, 1771, 2356
CviAII	CATG	8 cut(s)	118, 658, 898, 968, 1495, 2060, 2427, 2444
CviQI	GTAC	4 cut(s)	377, 1015, 1771, 2356
DdeI	CTNAG	4 cut(s)	198, 1279, 1295, 2054
DinI	GGCGCC	1 cut(s)	2022
DpnI	GATC	7 cut(s)	39, 203, 495, 701, 1655, 1698, 2403
DpnII	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
DraIII	CACNNNGTG	3 cut(s)	416, 687, 2167
EaeI	YGGCCR	2 cut(s)	396, 2432
EagI	CGGCCG	1 cut(s)	2432
Eam1104I	CTCTTC	4 cut(s)	807, 864, 1189, 1305
EarI	CTCTTC	4 cut(s)	807, 864, 1189, 1305
EciI	GGCGGA	8 cut(s)	276, 585, 664, 1059, 1220, 1378, 1450, 1576
Ecl136II	GAGCTC	1 cut(s)	1438
EclXI	CGGCCG	1 cut(s)	2432
Eco130I	CCWWGG	4 cut(s)	657, 1522, 1809, 2124
Eco147I	AGGCCT	5 cut(s)	582, 1344, 1756, 1808, 2321
Eco24I	GRGCYC	2 cut(s)	937, 1440
Eco31I	GGTCTC	2 cut(s)	1588, 1721
Eco47I	GGWCC	5 cut(s)	485, 669, 709, 920, 1903
Eco52I	CGGCCG	1 cut(s)	2432
Eco53kI	GAGCTC	1 cut(s)	1438
Eco57I	CTGAAG	2 cut(s)	1509, 1940
Eco72I	CACGTG	1 cut(s)	2164
Eco88I	CYCGRG	2 cut(s)	1727, 2333
EcoICRI	GAGCTC	1 cut(s)	1438
EcoO109I	RGGNCCY	3 cut(s)	485, 669, 709
EcoRII	CCWGG	9 cut(s)	412, 479, 922, 928, 1656, 1743, 1751, 1756, 2295
EcoT14I	CCWWGG	4 cut(s)	657, 1522, 1809, 2124
EcoT38I	GRGCYC	2 cut(s)	937, 1440
EgeI	GGCGCC	1 cut(s)	2022
EheI	GGCGCC	1 cut(s)	2022
ErhI	CCWWGG	4 cut(s)	657, 1522, 1809, 2124
Esp3I	CGTCTC	3 cut(s)	739, 1326, 1390
FaeI	CATG	8 cut(s)	121, 661, 901, 971, 1498, 2063, 2430, 2447
FaqI	GGGAC	3 cut(s)	1048, 1541, 2392
FatI	CATG	8 cut(s)	117, 657, 897, 967, 1494, 2059, 2426, 2443
FauI	CCCGC	8 cut(s)	260, 269, 1240, 1269, 1511, 1665, 2056, 2293
FokI	GGATG	4 cut(s)	725, 1177, 1727, 2350
FriOI	GRGCYC	2 cut(s)	937, 1440
FspBI	CTAG	3 cut(s)	624, 1523, 1853
GlaI	GCGC	3 cut(s)	1375, 1469, 2022
GsaI	CCCAGC	3 cut(s)	748, 792, 1468
GsuI	CTGGAG	5 cut(s)	827, 1053, 1735, 2170, 2275
HaeII	RGCGCY	1 cut(s)	2024
HgaI	GACGC	3 cut(s)	142, 183, 1459
HhaI	GCGC	3 cut(s)	1376, 1470, 2023
Hin1I	GRCGYC	4 cut(s)	194, 407, 1974, 2021
Hin1II	CATG	8 cut(s)	121, 661, 901, 971, 1498, 2063, 2430, 2447
Hin6I	GCGC	3 cut(s)	1374, 1468, 2021
HinP1I	GCGC	3 cut(s)	1374, 1468, 2021
HindIII	AAGCTT	1 cut(s)	871
HinfI	GANTC	8 cut(s)	816, 953, 1208, 1779, 1988, 2120, 2194, 2380
Hpy166II	GTNNAC	4 cut(s)	718, 1212, 1687, 2358
Hpy188III	TCNNGA	6 cut(s)	806, 950, 1032, 1700, 2187, 2335
Hpy8I	GTNNAC	4 cut(s)	718, 1212, 1687, 2358
Hpy99I	CGWCG	9 cut(s)	158, 535, 538, 649, 1406, 1475, 1697, 1976, 2227
HpyCH4III	ACNGT	2 cut(s)	997, 2038
HpyCH4IV	ACGT	7 cut(s)	407, 732, 972, 1773, 1974, 2163, 2202
HpyF3I	CTNAG	4 cut(s)	198, 1279, 1295, 2054
HpySE526I	ACGT	7 cut(s)	407, 732, 972, 1773, 1974, 2163, 2202
Hsp92I	GRCGYC	4 cut(s)	194, 407, 1974, 2021
Hsp92II	CATG	8 cut(s)	121, 661, 901, 971, 1498, 2063, 2430, 2447
HspAI	GCGC	3 cut(s)	1374, 1468, 2021
KasI	GGCGCC	1 cut(s)	2020
KroI	GCCGGC	1 cut(s)	473
KroNI	GCCGGC	1 cut(s)	475
KspI	CCGCGG	1 cut(s)	268
Kzo9I	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	9 cut(s)	728, 1037, 1435, 1520, 1553, 2044, 2129, 2170, 2229
LweI	GCATC	3 cut(s)	51, 1536, 1617
MabI	ACCWGGT	1 cut(s)	412
MaeI	CTAG	3 cut(s)	624, 1523, 1853
MaeII	ACGT	7 cut(s)	407, 732, 972, 1773, 1974, 2163, 2202
MaeIII	GTNAC	8 cut(s)	23, 62, 369, 408, 429, 1975, 2032, 2198
MalI	GATC	7 cut(s)	39, 203, 495, 701, 1655, 1698, 2403
MbiI	CCGCTC	3 cut(s)	800, 1672, 2232
MboI	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
MflI	RGATCY	1 cut(s)	2401
MhlI	GDGCHC	3 cut(s)	16, 937, 1440
MluCI	AATT	3 cut(s)	136, 391, 1149
Mly113I	GGCGCC	1 cut(s)	2021
MlyI	GAGTC	5 cut(s)	810, 947, 1217, 1773, 2374
MmeI	TCCRAC	4 cut(s)	165, 905, 1155, 1860
MroNI	GCCGGC	1 cut(s)	473
MroXI	GAANNNNTTC	1 cut(s)	106
MseI	TTAA	4 cut(s)	276, 876, 939, 2385
MslI	CAYNNNNRTG	1 cut(s)	2363
MspA1I	CMGCKG	6 cut(s)	48, 267, 464, 838, 1226, 2179
MspCI	CTTAAG	1 cut(s)	938
Mva1269I	GAATGC	1 cut(s)	1960
MvaI	CCWGG	9 cut(s)	414, 481, 924, 930, 1658, 1745, 1753, 1758, 2297
MvnI	CGCG	5 cut(s)	267, 1376, 1470, 1475, 1844
NaeI	GCCGGC	1 cut(s)	475
NarI	GGCGCC	1 cut(s)	2021
NciI	CCSGG	4 cut(s)	1728, 1729, 1907, 2278
NcoI	CCATGG	1 cut(s)	657
NdeII	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
NgoMIV	GCCGGC	1 cut(s)	473
NlaIII	CATG	8 cut(s)	121, 661, 901, 971, 1498, 2063, 2430, 2447
NlaIV	GGNNCC	8 cut(s)	478, 486, 670, 1456, 1795, 1905, 1984, 2022
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	4 cut(s)	23, 408, 429, 1975
NotI	GCGGCCGC	1 cut(s)	2432
OliI	CACNNNNGTG	1 cut(s)	2363
PaqCI	CACCTGC	1 cut(s)	1109
PasI	CCCWGGG	1 cut(s)	929
PceI	AGGCCT	5 cut(s)	582, 1344, 1756, 1808, 2321
PciSI	GCTCTTC	1 cut(s)	807
PcsI	WCGNNNNNNNCGW	1 cut(s)	1698
PctI	GAATGC	1 cut(s)	1960
PdiI	GCCGGC	1 cut(s)	475
PdmI	GAANNNNTTC	1 cut(s)	106
PfeI	GAWTC	3 cut(s)	1988, 2120, 2194
Pfl23II	CGTACG	1 cut(s)	1770
PflFI	GACNNNGTC	1 cut(s)	713
PflMI	CCANNNNNTGG	1 cut(s)	687
PfoI	TCCNGGA	1 cut(s)	1656
Ple19I	CGATCG	1 cut(s)	40
PleI	GAGTC	5 cut(s)	810, 947, 1216, 1773, 2374
PluTI	GGCGCC	1 cut(s)	2024
PmaCI	CACGTG	1 cut(s)	2164
PmlI	CACGTG	1 cut(s)	2164
PpsI	GAGTC	5 cut(s)	810, 947, 1216, 1773, 2374
Ppu21I	YACGTR	2 cut(s)	1774, 2164
PpuMI	RGGWCCY	3 cut(s)	485, 669, 709
PshAI	GACNNNNGTC	1 cut(s)	820
PshBI	ATTAAT	1 cut(s)	276
Psp124BI	GAGCTC	1 cut(s)	1440
Psp1406I	AACGTT	1 cut(s)	2202
Psp5II	RGGWCCY	3 cut(s)	485, 669, 709
Psp6I	CCWGG	9 cut(s)	412, 479, 922, 928, 1656, 1743, 1751, 1756, 2295
PspCI	CACGTG	1 cut(s)	2164
PspFI	CCCAGC	3 cut(s)	744, 788, 1464
PspGI	CCWGG	9 cut(s)	412, 479, 922, 928, 1656, 1743, 1751, 1756, 2295
PspLI	CGTACG	1 cut(s)	1770
PspN4I	GGNNCC	8 cut(s)	478, 486, 670, 1456, 1795, 1905, 1984, 2022
PspPPI	RGGWCCY	3 cut(s)	485, 669, 709
PstI	CTGCAG	1 cut(s)	469
PstNI	CAGNNNCTG	2 cut(s)	1518, 2179
PsuI	RGATCY	1 cut(s)	2401
PsyI	GACNNNGTC	1 cut(s)	713
PvuI	CGATCG	1 cut(s)	40
PvuII	CAGCTG	2 cut(s)	1226, 2179
RgaI	GCGATCGC	1 cut(s)	40
RsaI	GTAC	4 cut(s)	378, 1016, 1772, 2357
RsaNI	GTAC	4 cut(s)	377, 1015, 1771, 2356
RseI	CAYNNNNRTG	1 cut(s)	2363
SacI	GAGCTC	1 cut(s)	1440
SacII	CCGCGG	1 cut(s)	268
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	4 cut(s)	276, 876, 939, 2385
Sau3AI	GATC	7 cut(s)	37, 201, 493, 699, 1653, 1696, 2401
SchI	GAGTC	5 cut(s)	810, 947, 1217, 1773, 2374
SduI	GDGCHC	3 cut(s)	16, 937, 1440
SexAI	ACCWGGT	1 cut(s)	412
SfaAI	GCGATCGC	1 cut(s)	40
SfaNI	GCATC	3 cut(s)	51, 1536, 1617
SfcI	CTRYAG	1 cut(s)	465
SfiI	GGCCNNNNNGGCC	2 cut(s)	1762, 1814
SfoI	GGCGCC	1 cut(s)	2022
Sfr303I	CCGCGG	1 cut(s)	268
SgfI	GCGATCGC	1 cut(s)	40
SgrBI	CCGCGG	1 cut(s)	268
SinI	GGWCC	5 cut(s)	485, 669, 709, 920, 1903
SmaI	CCCGGG	1 cut(s)	1729
SmiMI	CAYNNNNRTG	1 cut(s)	2363
SmlI	CTYRAG	1 cut(s)	938
SmoI	CTYRAG	1 cut(s)	938
SpeI	ACTAGT	1 cut(s)	623
SrfI	GCCCGGGC	1 cut(s)	1729
Sse9I	AATT	3 cut(s)	136, 391, 1149
SseBI	AGGCCT	5 cut(s)	582, 1344, 1756, 1808, 2321
SspDI	GGCGCC	1 cut(s)	2020
SspMI	CTAG	3 cut(s)	624, 1523, 1853
SstI	GAGCTC	1 cut(s)	1440
StuI	AGGCCT	5 cut(s)	582, 1344, 1756, 1808, 2321
StyI	CCWWGG	4 cut(s)	657, 1522, 1809, 2124
TaaI	ACNGT	2 cut(s)	997, 2038
TaiI	ACGT	7 cut(s)	410, 735, 975, 1776, 1977, 2166, 2205
TaqI	TCGA	9 cut(s)	156, 204, 492, 1401, 1425, 1668, 1695, 1701, 2225
TasI	AATT	3 cut(s)	136, 391, 1149
TatI	WGTACW	1 cut(s)	1014
TfiI	GAWTC	3 cut(s)	1988, 2120, 2194
Tru1I	TTAA	4 cut(s)	276, 876, 939, 2385
Tru9I	TTAA	4 cut(s)	276, 876, 939, 2385
TscAI	CASTG	3 cut(s)	1252, 1261, 2217
TseFI	GTSAC	4 cut(s)	23, 408, 429, 1975
Tsp45I	GTSAC	4 cut(s)	23, 408, 429, 1975
TspDTI	ATGAA	7 cut(s)	134, 497, 743, 1142, 1176, 1609, 2432
TspGWI	ACGGA	4 cut(s)	633, 640, 1543, 1758
TspMI	CCCGGG	1 cut(s)	1727
TspRI	CASTG	3 cut(s)	1252, 1261, 2217
Tth111I	GACNNNGTC	1 cut(s)	713
Van91I	CCANNNNNTGG	1 cut(s)	687
Vha464I	CTTAAG	1 cut(s)	938
VpaK11BI	GGWCC	5 cut(s)	485, 669, 709, 920, 1903
VspI	ATTAAT	1 cut(s)	276
XapI	RAATTY	1 cut(s)	391
XmaI	CCCGGG	1 cut(s)	1727
XmaJI	CCTAGG	1 cut(s)	1522
XmnI	GAANNNNTTC	1 cut(s)	106
XspI	CTAG	3 cut(s)	624, 1523, 1853
ZraI	GACGTC	2 cut(s)	408, 1975

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.