MD04G1207600.v1.1

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012048
Physical Location & Seq
Reverse (-)
29274566 .. 29277830
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1207600.v1.1.491

Sequence Viewer

Length: 1641 bp
ATGGCCATCTCTCTCTCCTCCTCTTTCAAAACCTGCATTCTCTCACTCTCTCTCCTCTCCTTCTTCCTCTTCTTCCTCCTCACAACCCCAACTCCCAATTCCCCAAACTACCCTAACCCATCTCCCATAACGTTCCACAGGTCCCTCATCGCCACCGCAGCAAAAACCCCATCTTGCTCCTCCGCCGCCGTAACCCACTCCGATGGCCTCTTTAACTACCTCTACCTCCACTTCTGCCTCTTCCGCCAAAACCCATTTCTCTCTCTCTCCTCCCTCTCTCTTATCCTCCTCCTCCTCTTCTACATCCTCATCAAAACAGCGCAGGATCACTTCTCTCTCGTCACCACCAAGCTCGTTTTTGCCCTCGATTTGACCCCCAGCATGGCCGCCGTCACCCTCCTCGCGCTCGGAAACGGCGCCCCTGACGTCTTTGCCTCGGTCGCGGCGGTCCGGACGGGGCAGTACCGAACCGGGTTCGGCGCGATTCTGTCCGCCGGCACTTTCGTGTCGGCGTTCGTGGTGGGGTTTGTGGCGATTTACGCCGCGCCCTTTCCGCTCAAGCCGGCGCCGTTTGTGAGGGACGTGATGTTTTATCTGACGGCGGCGCTGTTCTTGTTCTATGTGTATCTGAGCGCCGAGATTTATCTGTGGCAAGCGGTCGGGTTTGTTGGGTTTTACTTATTTTTTGTTGGGTTGGTGTTTTGGATGGATTTGGGAATGGGAGGAGGAGGAAAGGAGAGCAGAGTTGAGGCGGGATCGGTCGGAGACATTGAAATTCAGAGGGGTTTGGTTGCACCAGATTCCAGGATCGGACATGTTTTGAAGAGTTCAGAGGATAGGAAGGGGAGTTTTGGATTTGGGAGAGCTCTTGGAATGATCTCGAGAGCATGGGAGCTTCCCGTCTCATTTCTTTTGAAGCTCACAATTCCCCAAGCTTCACCTTCCGAATGGAGCAGATTCTACATGTCAGCCAATATTGCTCTTTGCCCTCTCGCGCTGCTATATGCTTGCAACTCATTCATGCCATTTAATCACCCCGTTGTTTTTCTCCTTTCAAACACCCATTTGCCCCTATGGCTTGTAATCCTCTTGGCAAGCTCCTCTCTCGCACTTCTTCATTATGCAGTAGAAAAAGACCCCCCCAAAACCGAGCAACTGCCTGTATTGCTTATAGCATTTGTTATGAGTGTCTTTTGGATATCTACAACTGCTGGTGAATTGCTGAACTGCCTCGCTGCACTCGGAACACTTCTGGAGTTGCCACCTGCACTTCTTGGGCTCACAGTTCTTGCATGGGGAAATTCAGTTGGGGATCTTGTTGCTGATGTTGCAGTTGCCAAGGCTGGCCACCCTGCAATGGCCATGGCTGGATGTTTTGCTGGGCCAATGTTTAACATGCTCATTGGACTTGGAACTGCCTTGGTTATACAGACGTATGATGTCTATCCCGAGGCATACGAGCTTCAGTTCCACGTGGGTATAGTGATTGCATTTGTTTTCTTGCTTCTGAGCCTGATGGGGTCTCTATTGGTGATTACATGGTGCAGATTTCGGGTGCCTAGGTTCTGGGGATTCTGTCTTGTTGGTCTCTATGTTGTTTTTATGGCAGTTAGTTTGGTAATCGCCAAGTTTACAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0003712 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009987 GO:0010029 GO:0010033 GO:0010099 GO:0010228 GO:0010468 GO:0010476 GO:0010556 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015318 GO:0015672 GO:0016020 GO:0019219 GO:0019222 GO:0022414 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034220 GO:0034399 GO:0035725 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046873 GO:0048518 GO:0048571 GO:0048573 GO:0048574 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051716 GO:0055085 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071804 GO:0071805 GO:0071944 GO:0080090 GO:0097305 GO:0097306 GO:0098655 GO:0098660 GO:0098662 GO:0140110 GO:1900140 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000030 GO:2000112 GO:2000306 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

547

Amino Acids

59.29

Weight (kDa)

8.02

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 93 - 235 1.6e-20 Sodium/calcium exchanger protein
Na_Ca_ex PF01699 389 - 539 3.4e-30 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08960
fragaria_vesca FvH4_6g05530 FvH4_6g05530 FvH4_6g05530
malus_domestica MD04G1207600.v1.1 MD12G1221900.v1.1
prunus_persica Prupe.6G328600_v2.0.a1
pyrus_communis pycom04g18380 pycom12g20800
rosa_chinensis RchiOBHm_Chr3g0454301
rosa_laevigata RLG00000025464
rosa_multiflora Rmu_sc0001340.1_g000003
rosa_roxburghii Rroxscaffold_165G00437120
rosa_rugosa Rorug02G0654100
rosa_samantha Rh3AG058000 Rh3BG059700 Rh3CG058900 Rh3DG060200
rosa_wichuraiana Rw3G004440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1273
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 41, 1273
AccB1I GGYRCC 3 cut(s) 416, 565, 1555
AccBSI CCGCTC 1 cut(s) 556
AccII CGCG 5 cut(s) 404, 443, 482, 545, 995
AccIII TCCGGA 1 cut(s) 450
AclI AACGTT 1 cut(s) 131
AclWI GGATC 4 cut(s) 333, 763, 815, 1320
AcoI YGGCCR 4 cut(s) 3, 384, 1345, 1359
AcsI RAATTY 2 cut(s) 774, 1300
AcuI CTGAAG 1 cut(s) 1448
AcvI CACGTG 1 cut(s) 1474
AcyI GRCGYC 3 cut(s) 417, 426, 566
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 2 cut(s) 382, 1357
AflIII ACRYGT 2 cut(s) 814, 963
AgsI TTSAA 5 cut(s) 28, 773, 823, 916, 1056
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 1 cut(s) 803
AjuI GAANNNNNNNTTGG 4 cut(s) 82, 114, 240, 272
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 7 cut(s) 352, 866, 895, 919, 935, 1098, 1462
AluI AGCT 7 cut(s) 352, 866, 895, 919, 935, 1098, 1462
Alw21I GWGCWC 1 cut(s) 868
Alw26I GTCTC 4 cut(s) 759, 907, 1527, 1592
AlwI GGATC 4 cut(s) 333, 763, 815, 1320
Ama87I CYCGRG 2 cut(s) 880, 1448
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 6 cut(s) 3, 205, 384, 1345, 1359, 1382
ApeKI GCWGC 3 cut(s) 158, 997, 1235
ApoI RAATTY 2 cut(s) 774, 1300
AspA2I CCTAGG 1 cut(s) 1559
AspLEI GCGC 9 cut(s) 322, 406, 419, 482, 547, 568, 607, 635, 997
AspS9I GGNCC 3 cut(s) 141, 448, 1382
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 6 cut(s) 334, 385, 930, 1025, 1226, 1543
AvaI CYCGRG 2 cut(s) 880, 1448
AvaII GGWCC 2 cut(s) 141, 448
AvrII CCTAGG 1 cut(s) 1559
BalI TGGCCA 3 cut(s) 5, 1347, 1361
BanI GGYRCC 3 cut(s) 416, 565, 1555
BanII GRGCYC 2 cut(s) 868, 1281
BbrPI CACGTG 1 cut(s) 1474
Bbv12I GWGCWC 1 cut(s) 868
BbvI GCAGC 3 cut(s) 170, 984, 1222
BccI CCATC 6 cut(s) 14, 127, 178, 197, 700, 1510
BceAI ACGGC 5 cut(s) 173, 374, 430, 553, 615
BciT130I CCWGG 1 cut(s) 805
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 759, 907, 1527, 1592
BfaI CTAG 1 cut(s) 1560
BfoI RGCGCY 4 cut(s) 420, 569, 608, 636
BfuAI ACCTGC 2 cut(s) 41, 1273
BglI GCCNNNNNGGC 1 cut(s) 1075
BisI GCNGC 8 cut(s) 159, 186, 387, 444, 543, 603, 998, 1236
BlnI CCTAGG 1 cut(s) 1559
BlsI GCNGC 8 cut(s) 160, 187, 388, 445, 544, 604, 999, 1237
Bme1390I CCNGG 2 cut(s) 472, 805
Bme18I GGWCC 2 cut(s) 141, 448
BmeT110I CYCGRG 2 cut(s) 880, 1448
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 3 cut(s) 141, 448, 1382
BmiI GGNNCC 4 cut(s) 143, 418, 567, 1557
BmrFI CCNGG 2 cut(s) 472, 805
BpmI CTGGAG 1 cut(s) 1274
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 1474
BsaBI GATNNNNATC 1 cut(s) 1443
BsaHI GRCGYC 3 cut(s) 417, 426, 566
BsaI GGTCTC 2 cut(s) 1527, 1592
BsaJI CCNNGG 6 cut(s) 435, 1338, 1362, 1419, 1449, 1559
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 76, 106
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 1357
Bse118I RCCGGY 2 cut(s) 494, 562
Bse3DI GCAATG 1 cut(s) 1362
Bse8I GATNNNNATC 1 cut(s) 1443
BseAI TCCGGA 1 cut(s) 450
BseBI CCWGG 1 cut(s) 805
BseDI CCNNGG 6 cut(s) 435, 1338, 1362, 1419, 1449, 1559
BseGI GGATG 3 cut(s) 303, 711, 1376
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 2 cut(s) 382, 1357
BseMI GCAATG 1 cut(s) 1362
BseMII CTCAG 2 cut(s) 620, 1499
BseXI GCAGC 3 cut(s) 170, 984, 1222
BseYI CCCAGC 2 cut(s) 377, 1379
BsgI GTGCAG 3 cut(s) 1221, 1251, 1564
Bsh1236I CGCG 5 cut(s) 404, 443, 482, 545, 995
Bsh1285I CGRYCG 3 cut(s) 441, 660, 762
BshFI GGCC 6 cut(s) 5, 207, 386, 1347, 1361, 1384
BshNI GGYRCC 3 cut(s) 416, 565, 1555
BsiEI CGRYCG 3 cut(s) 441, 660, 762
BsiHKAI GWGCWC 1 cut(s) 868
BsiHKCI CYCGRG 2 cut(s) 880, 1448
BsiSI CCGG 4 cut(s) 451, 471, 495, 563
BslFI GGGAC 2 cut(s) 127, 593
BslI CCNNNNNNNGG 2 cut(s) 382, 1357
BsmAI GTCTC 4 cut(s) 759, 907, 1527, 1592
BsmBI CGTCTC 1 cut(s) 907
BsmFI GGGAC 2 cut(s) 127, 593
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 6 cut(s) 5, 207, 386, 1347, 1361, 1384
Bso31I GGTCTC 2 cut(s) 1527, 1592
BsoBI CYCGRG 2 cut(s) 880, 1448
Bsp1286I GDGCHC 2 cut(s) 868, 1281
Bsp13I TCCGGA 1 cut(s) 450
Bsp143I GATC 5 cut(s) 325, 755, 807, 876, 1312
Bsp19I CCATGG 1 cut(s) 1362
BspANI GGCC 6 cut(s) 5, 207, 386, 1347, 1361, 1384
BspCNI CTCAG 2 cut(s) 621, 1500
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 5 cut(s) 404, 443, 482, 545, 995
BspLI GGNNCC 4 cut(s) 143, 418, 567, 1557
BspMI ACCTGC 2 cut(s) 41, 1273
BspPI GGATC 4 cut(s) 333, 763, 815, 1320
BspT107I GGYRCC 3 cut(s) 416, 565, 1555
BspTNI GGTCTC 2 cut(s) 1527, 1592
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 1 cut(s) 1362
BsrFI RCCGGY 2 cut(s) 494, 562
BssAI RCCGGY 2 cut(s) 494, 562
BssECI CCNNGG 6 cut(s) 435, 1338, 1362, 1419, 1449, 1559
BssMI GATC 5 cut(s) 325, 755, 807, 876, 1312
BssNI GRCGYC 3 cut(s) 417, 426, 566
BssT1I CCWWGG 4 cut(s) 1338, 1362, 1419, 1559
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 1 cut(s) 1285
Bst6I CTCTTC 4 cut(s) 74, 245, 302, 818
BstACI GRCGYC 3 cut(s) 417, 426, 566
BstBAI YACGTR 1 cut(s) 1474
BstC8I GCNNGC 6 cut(s) 496, 564, 654, 1009, 1096, 1345
BstDEI CTNAG 2 cut(s) 629, 1508
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 3 cut(s) 303, 711, 1376
BstFNI CGCG 5 cut(s) 404, 443, 482, 545, 995
BstH2I RGCGCY 4 cut(s) 420, 569, 608, 636
BstHHI GCGC 9 cut(s) 322, 406, 419, 482, 547, 568, 607, 635, 997
BstKTI GATC 5 cut(s) 328, 758, 810, 879, 1315
BstMAI GTCTC 4 cut(s) 759, 907, 1527, 1592
BstMBI GATC 5 cut(s) 325, 755, 807, 876, 1312
BstMCI CGRYCG 3 cut(s) 441, 660, 762
BstMWI GCNNNNNNNGC 9 cut(s) 158, 243, 440, 539, 553, 977, 1075, 1165, 1328
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 3 cut(s) 818, 967, 1399
BstSCI CCNGG 2 cut(s) 470, 803
BstUI CGCG 5 cut(s) 404, 443, 482, 545, 995
BstV1I GCAGC 3 cut(s) 170, 984, 1222
BstX2I RGATCY 1 cut(s) 1312
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 1 cut(s) 1312
BsuRI GGCC 6 cut(s) 5, 207, 386, 1347, 1361, 1384
BtgI CCRYGG 1 cut(s) 1362
BtgZI GCGATG 1 cut(s) 133
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 3 cut(s) 303, 711, 1376
BveI ACCTGC 2 cut(s) 41, 1273
Cac8I GCNNGC 6 cut(s) 496, 564, 654, 1009, 1096, 1345
CfoI GCGC 9 cut(s) 322, 406, 419, 482, 547, 568, 607, 635, 997
Cfr10I RCCGGY 2 cut(s) 494, 562
Cfr13I GGNCC 3 cut(s) 141, 448, 1382
CpoI CGGWCCG 1 cut(s) 448
Csp6I GTAC 1 cut(s) 463
CspI CGGWCCG 1 cut(s) 448
CviAII CATG 9 cut(s) 382, 815, 888, 964, 1021, 1293, 1363, 1396, 1539
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 2 cut(s) 629, 1508
DinI GGCGCC 2 cut(s) 418, 567
DpnI GATC 5 cut(s) 327, 757, 809, 878, 1314
DpnII GATC 5 cut(s) 325, 755, 807, 876, 1312
EaeI YGGCCR 4 cut(s) 3, 384, 1345, 1359
Eam1104I CTCTTC 4 cut(s) 74, 245, 302, 818
EarI CTCTTC 4 cut(s) 74, 245, 302, 818
EciI GGCGGA 3 cut(s) 172, 233, 481
Ecl136II GAGCTC 1 cut(s) 866
Eco130I CCWWGG 4 cut(s) 1338, 1362, 1419, 1559
Eco24I GRGCYC 2 cut(s) 868, 1281
Eco31I GGTCTC 2 cut(s) 1527, 1592
Eco32I GATATC 1 cut(s) 1200
Eco47I GGWCC 2 cut(s) 141, 448
Eco53kI GAGCTC 1 cut(s) 866
Eco57I CTGAAG 1 cut(s) 1448
Eco72I CACGTG 1 cut(s) 1474
Eco88I CYCGRG 2 cut(s) 880, 1448
EcoICRI GAGCTC 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 803
EcoRV GATATC 1 cut(s) 1200
EcoT14I CCWWGG 4 cut(s) 1338, 1362, 1419, 1559
EcoT38I GRGCYC 2 cut(s) 868, 1281
EgeI GGCGCC 2 cut(s) 418, 567
EheI GGCGCC 2 cut(s) 418, 567
ErhI CCWWGG 4 cut(s) 1338, 1362, 1419, 1559
Esp3I CGTCTC 1 cut(s) 907
FaeI CATG 9 cut(s) 385, 818, 891, 967, 1024, 1296, 1366, 1399, 1542
FaqI GGGAC 2 cut(s) 127, 593
FatI CATG 9 cut(s) 381, 814, 887, 963, 1020, 1292, 1362, 1395, 1538
FauI CCCGC 1 cut(s) 745
Fnu4HI GCNGC 8 cut(s) 159, 186, 387, 444, 543, 603, 998, 1236
FokI GGATG 3 cut(s) 290, 718, 1383
FriOI GRGCYC 2 cut(s) 868, 1281
Fsp4HI GCNGC 8 cut(s) 159, 186, 387, 444, 543, 603, 998, 1236
FspBI CTAG 1 cut(s) 1560
GlaI GCGC 9 cut(s) 321, 405, 418, 481, 546, 567, 606, 634, 996
GluI GCNGC 8 cut(s) 159, 186, 387, 444, 543, 603, 998, 1236
GsaI CCCAGC 2 cut(s) 381, 1383
GsuI CTGGAG 1 cut(s) 1274
HaeII RGCGCY 4 cut(s) 420, 569, 608, 636
HaeIII GGCC 6 cut(s) 5, 207, 386, 1347, 1361, 1384
HapII CCGG 4 cut(s) 451, 471, 495, 563
HhaI GCGC 9 cut(s) 322, 406, 419, 482, 547, 568, 607, 635, 997
Hin1I GRCGYC 3 cut(s) 417, 426, 566
Hin1II CATG 9 cut(s) 385, 818, 891, 967, 1024, 1296, 1366, 1399, 1542
Hin6I GCGC 9 cut(s) 320, 404, 417, 480, 545, 566, 605, 633, 995
HinP1I GCGC 9 cut(s) 320, 404, 417, 480, 545, 566, 605, 633, 995
HindIII AAGCTT 1 cut(s) 933
HinfI GANTC 4 cut(s) 484, 800, 957, 1572
HpaII CCGG 4 cut(s) 451, 471, 495, 563
HphI GGTGA 6 cut(s) 334, 385, 930, 1025, 1226, 1543
Hpy166II GTNNAC 1 cut(s) 1632
Hpy188III TCNNGA 5 cut(s) 451, 880, 882, 1253, 1448
Hpy8I GTNNAC 1 cut(s) 1632
HpyAV CCTTC 3 cut(s) 70, 835, 951
HpyCH4III ACNGT 1 cut(s) 1285
HpyCH4IV ACGT 5 cut(s) 131, 426, 582, 1433, 1473
HpyF10VI GCNNNNNNNGC 9 cut(s) 158, 243, 440, 539, 553, 977, 1075, 1165, 1328
HpyF3I CTNAG 2 cut(s) 629, 1508
HpySE526I ACGT 5 cut(s) 131, 426, 582, 1433, 1473
Hsp92I GRCGYC 3 cut(s) 417, 426, 566
Hsp92II CATG 9 cut(s) 385, 818, 891, 967, 1024, 1296, 1366, 1399, 1542
HspAI GCGC 9 cut(s) 320, 404, 417, 480, 545, 566, 605, 633, 995
KasI GGCGCC 2 cut(s) 416, 565
Kpn2I TCCGGA 1 cut(s) 450
KroI GCCGGC 2 cut(s) 494, 562
KroNI GCCGGC 2 cut(s) 496, 564
Kzo9I GATC 5 cut(s) 325, 755, 807, 876, 1312
LmnI GCTCC 4 cut(s) 182, 892, 951, 1103
Lsp1109I GCAGC 3 cut(s) 170, 984, 1222
MaeI CTAG 1 cut(s) 1560
MaeII ACGT 5 cut(s) 131, 426, 582, 1433, 1473
MaeIII GTNAC 3 cut(s) 190, 340, 391
MalI GATC 5 cut(s) 327, 757, 809, 878, 1314
MbiI CCGCTC 1 cut(s) 556
MboI GATC 5 cut(s) 325, 755, 807, 876, 1312
MboII GAAGA 7 cut(s) 55, 61, 64, 232, 289, 835, 1106
MflI RGATCY 1 cut(s) 1312
MhlI GDGCHC 2 cut(s) 868, 1281
MlsI TGGCCA 3 cut(s) 5, 1347, 1361
MluCI AATT 5 cut(s) 97, 774, 924, 1217, 1300
MluNI TGGCCA 3 cut(s) 5, 1347, 1361
Mly113I GGCGCC 2 cut(s) 417, 566
MmeI TCCRAC 1 cut(s) 742
Mox20I TGGCCA 3 cut(s) 5, 1347, 1361
MroI TCCGGA 1 cut(s) 450
MroNI GCCGGC 2 cut(s) 494, 562
MscI TGGCCA 3 cut(s) 5, 1347, 1361
MseI TTAA 3 cut(s) 213, 1029, 1392
MslI CAYNNNNRTG 2 cut(s) 201, 503
Msp20I TGGCCA 3 cut(s) 5, 1347, 1361
MspI CCGG 4 cut(s) 451, 471, 495, 563
MspR9I CCNGG 2 cut(s) 472, 805
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 5 cut(s) 404, 443, 482, 545, 995
MwoI GCNNNNNNNGC 9 cut(s) 158, 243, 440, 539, 553, 977, 1075, 1165, 1328
NaeI GCCGGC 2 cut(s) 496, 564
NarI GGCGCC 2 cut(s) 417, 566
NciI CCSGG 1 cut(s) 472
NcoI CCATGG 1 cut(s) 1362
NdeII GATC 5 cut(s) 325, 755, 807, 876, 1312
NgoMIV GCCGGC 2 cut(s) 494, 562
NlaIII CATG 9 cut(s) 385, 818, 891, 967, 1024, 1296, 1366, 1399, 1542
NlaIV GGNNCC 4 cut(s) 143, 418, 567, 1557
NmeAIII GCCGAG 1 cut(s) 661
NmuCI GTSAC 2 cut(s) 340, 391
NspI RCATGY 3 cut(s) 818, 967, 1399
OliI CACNNNNGTG 1 cut(s) 503
PaeR7I CTCGAG 1 cut(s) 880
PaqCI CACCTGC 1 cut(s) 1273
PciI ACATGT 2 cut(s) 814, 963
PctI GAATGC 1 cut(s) 36
PdiI GCCGGC 2 cut(s) 496, 564
PfeI GAWTC 4 cut(s) 484, 800, 957, 1572
PfoI TCCNGGA 1 cut(s) 803
PkrI GCNGC 8 cut(s) 160, 187, 388, 445, 544, 604, 999, 1237
PluTI GGCGCC 2 cut(s) 420, 569
PmaCI CACGTG 1 cut(s) 1474
PmlI CACGTG 1 cut(s) 1474
Ppu21I YACGTR 1 cut(s) 1474
PpuMI RGGWCCY 1 cut(s) 141
PscI ACATGT 2 cut(s) 814, 963
Psp124BI GAGCTC 1 cut(s) 868
Psp1406I AACGTT 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 803
PspCI CACGTG 1 cut(s) 1474
PspFI CCCAGC 2 cut(s) 377, 1379
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 4 cut(s) 143, 418, 567, 1557
PspPI GGNCC 3 cut(s) 141, 448, 1382
PspPPI RGGWCCY 1 cut(s) 141
PsuI RGATCY 1 cut(s) 1312
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 2 cut(s) 201, 503
Rsr2I CGGWCCG 1 cut(s) 448
RsrII CGGWCCG 1 cut(s) 448
SacI GAGCTC 1 cut(s) 868
SaqAI TTAA 3 cut(s) 213, 1029, 1392
SatI GCNGC 8 cut(s) 159, 186, 387, 444, 543, 603, 998, 1236
Sau3AI GATC 5 cut(s) 325, 755, 807, 876, 1312
Sau96I GGNCC 3 cut(s) 141, 448, 1382
ScrFI CCNGG 2 cut(s) 472, 805
SduI GDGCHC 2 cut(s) 868, 1281
SfoI GGCGCC 2 cut(s) 418, 567
Sfr274I CTCGAG 1 cut(s) 880
SinI GGWCC 2 cut(s) 141, 448
SlaI CTCGAG 1 cut(s) 880
SmiMI CAYNNNNRTG 2 cut(s) 201, 503
SmlI CTYRAG 2 cut(s) 557, 880
SmoI CTYRAG 2 cut(s) 557, 880
Sse9I AATT 5 cut(s) 97, 774, 924, 1217, 1300
SspDI GGCGCC 2 cut(s) 416, 565
SspI AATATT 1 cut(s) 976
SspMI CTAG 1 cut(s) 1560
SstI GAGCTC 1 cut(s) 868
StyD4I CCNGG 2 cut(s) 470, 803
StyI CCWWGG 4 cut(s) 1338, 1362, 1419, 1559
TaaI ACNGT 1 cut(s) 1285
TaiI ACGT 5 cut(s) 134, 429, 585, 1436, 1476
TaqI TCGA 2 cut(s) 366, 881
TaqII GACCGA 2 cut(s) 427, 748
TasI AATT 5 cut(s) 97, 774, 924, 1217, 1300
TauI GCSGC 5 cut(s) 188, 389, 446, 545, 605
TfiI GAWTC 4 cut(s) 484, 800, 957, 1572
Tru1I TTAA 3 cut(s) 213, 1029, 1392
Tru9I TTAA 3 cut(s) 213, 1029, 1392
TseFI GTSAC 2 cut(s) 340, 391
TseI GCWGC 3 cut(s) 158, 997, 1235
Tsp45I GTSAC 2 cut(s) 340, 391
TspDTI ATGAA 2 cut(s) 1009, 1106
VpaK11BI GGWCC 2 cut(s) 141, 448
XapI RAATTY 2 cut(s) 774, 1300
XceI RCATGY 3 cut(s) 818, 967, 1399
XhoI CTCGAG 1 cut(s) 880
XmaJI CCTAGG 1 cut(s) 1559
XspI CTAG 1 cut(s) 1560
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.