MD04G1222600.v1.1

PITH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013361
Physical Location & Seq
Forward (+)
30333177 .. 30335909
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1222600.v1.1.491

Sequence Viewer

Length: 534 bp
ATGTCTGCAGAATCGGCTAGTGCAATCCAAAGAAGCCAAGTTGATCTATTGGACTTCATAGATTGGTCAGGTGTTGAATGCCTCAACCAAAGCACTTCTCACTCTGTTGCCAACGCTCTCAAGCAGGGTTATAGAGAAGATGATGGTTTGAATCTGGAGAGCGATGCGGATGAGCAGCTTTTGATTTATATACCTTTTCTTCAAGTCGTAAAACTACACTCTATCATCGTCAAGGGGCCTGAAGACGAAGGTCCCAAGACCGTGAAACTTTTTTCAAACAAGGAGCACATGGGATTCAGCAACGTCAATGACTACCCAGCAAGTGACACCATTGATTTATCCCCTGATAATTTAAAGGGAAAACCAGTTGTTTTGAAGTATGTCAAGTTTCAAAATGTTCGCAGCTTGACTGTTTTTATCGAGGACAATCAATCCGGCTCAGAAAGCACAAAAGTTCAAAAGATTGTTCTTTGTGGAACAACGGTGGAAACGACAGACATGAAGGGCTTGAAGAAAATTGAGGACGGACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

178

Amino Acids

19.56

Weight (kDa)

4.87

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 8 - 173 1.3e-54 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04780
fragaria_vesca FvH4_6g03140
malus_domestica MD04G1222600.v1.1 MD12G1238700.v1.1
prunus_persica Prupe.6G341600_v2.0.a1
pyrus_communis pycom04g19690 pycom12g21940
rosa_chinensis RchiOBHm_Chr3g0451391
rosa_laevigata RLG00000025663
rosa_multiflora Rmu_sc0021369.1_g000001
rosa_roxburghii Rroxscaffold_164G00436530 Rroxscaffold_6G00428810
rosa_rugosa Rorug02G0633500
rosa_samantha Rh3AG036500 Rh3BG037100 Rh3CG035700 Rh3DG036600
rosa_wichuraiana Rw3G002690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 261
AgsI TTSAA 8 cut(s) 77, 151, 203, 276, 376, 392, 458, 511
AluBI AGCT 2 cut(s) 178, 405
AluI AGCT 2 cut(s) 178, 405
Alw21I GWGCWC 1 cut(s) 288
AoxI GGCC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 175, 402
AspS9I GGNCC 2 cut(s) 236, 251
AvaII GGWCC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 187, 414
BccI CCATC 1 cut(s) 137
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 2 cut(s) 176, 403
BlsI GCNGC 2 cut(s) 177, 404
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 236, 251
BmiI GGNNCC 2 cut(s) 237, 253
BmsI GCATC 1 cut(s) 154
BoxI GACNNNNGTC 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 365
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 187, 414
BseYI CCCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 237
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 1 cut(s) 43
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 238
BspCNI CTCAG 1 cut(s) 452
BspLI GGNNCC 2 cut(s) 237, 253
BspMAI CTGCAG 1 cut(s) 10
BsrI ACTGG 1 cut(s) 365
BssMI GATC 1 cut(s) 43
Bst4CI ACNGT 3 cut(s) 262, 412, 484
BstDEI CTNAG 1 cut(s) 439
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 1 cut(s) 46
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 14, 444
BstPAI GACNNNNGTC 1 cut(s) 249
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 187, 414
BstV2I GAAGAC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 238
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 529
Cfr13I GGNCC 2 cut(s) 236, 251
CviAII CATG 2 cut(s) 289, 499
CviJI RGCY 7 cut(s) 17, 36, 178, 238, 405, 438, 507
CviKI_1 RGCY 7 cut(s) 17, 36, 178, 238, 405, 438, 507
DdeI CTNAG 1 cut(s) 439
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
DraI TTTAAA 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 261
EcoO109I RGGNCCY 2 cut(s) 236, 251
FaeI CATG 2 cut(s) 292, 502
FaiI YATR 7 cut(s) 59, 132, 189, 191, 290, 381, 500
FaqI GGGAC 1 cut(s) 237
FatI CATG 2 cut(s) 288, 498
Fnu4HI GCNGC 2 cut(s) 176, 403
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 176, 403
FspBI CTAG 1 cut(s) 18
GluI GCNGC 2 cut(s) 176, 403
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 238
HapII CCGG 1 cut(s) 435
Hin1II CATG 2 cut(s) 292, 502
HinfI GANTC 3 cut(s) 11, 151, 294
HpaII CCGG 1 cut(s) 435
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 242, 496
HpyCH4III ACNGT 3 cut(s) 262, 412, 484
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 8, 23
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 444
HpyF3I CTNAG 1 cut(s) 439
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 2 cut(s) 292, 502
Kzo9I GATC 1 cut(s) 43
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 8 cut(s) 54, 110, 140, 252, 330, 357, 378, 448
Lsp1109I GCAGC 2 cut(s) 187, 414
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 18
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 323
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MboII GAAGA 4 cut(s) 149, 191, 254, 523
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 2 cut(s) 349, 516
MnlI CCTC 3 cut(s) 92, 415, 514
MseI TTAA 1 cut(s) 353
MspI CCGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 14, 444
NdeII GATC 1 cut(s) 43
NlaIII CATG 2 cut(s) 292, 502
NlaIV GGNNCC 2 cut(s) 237, 253
NmuCI GTSAC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 3 cut(s) 11, 151, 294
PkrI GCNGC 2 cut(s) 177, 404
PpuMI RGGWCCY 1 cut(s) 251
PshAI GACNNNNGTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 237, 253
PspPI GGNCC 2 cut(s) 236, 251
PspPPI RGGWCCY 1 cut(s) 251
PstI CTGCAG 1 cut(s) 10
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 2 cut(s) 176, 403
Sau3AI GATC 1 cut(s) 43
Sau96I GGNCC 2 cut(s) 236, 251
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 73, 180, 196, 253, 306, 407
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 349, 516
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 18
TaaI ACNGT 3 cut(s) 262, 412, 484
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 420
TasI AATT 2 cut(s) 349, 516
TfiI GAWTC 3 cut(s) 11, 151, 294
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 2 cut(s) 175, 402
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 2 cut(s) 46, 515
VpaK11BI GGWCC 1 cut(s) 251
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.