MD12G1238700.v1.1

PITH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013361
Physical Location & Seq
Forward (+)
31173360 .. 31176273
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1238700.v1.1.491

Sequence Viewer

Length: 534 bp
ATGTCTGCGGAATCAGCCAGTGCAATCCAAAAAAGCCAAGTTGATTTATTGGACTTCATAGATTGGTCTGGTGTTGAATGCCTCAACCAAAGCACCTCTCACTCTGTTGCTAACGCTCTCAAGCAGGGTTATAGAGAAGATGATGGTTTGAATCTGGAGAGCGATGCGGATGAGCAGCTTTTGATTTACATACCTTTTCTTCAAGTCGTTAAACTGCACTCTGTCGTCGTCAAAGGACCTGAAGATGAAGGTCCTAAGACCGTGAAACTTTTTTCAAACAAGGAGCACATGGGATTCAGCAATGTCAATGACTACCCAGCAAGTGACACCATTGATTTATCCCCTGATAATCTAAAGGGAAAACCAGTTGTTTTGAAGTATGTCAAGTTTCAAAATGTTCGCAGCTTGACTGTTTTTATCGAGGACAATCAATCCGGCTCGGAAGTCACAAAAGTTCAAAAGATTGTTCTTTGTGGAACAACGGTGGAAACGACAGACATGAAGGGCTTGAAGAAAATTGAGGACGGACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

178

Amino Acids

19.52

Weight (kDa)

4.87

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 8 - 173 5.1e-54 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04780
fragaria_vesca FvH4_6g03140
malus_domestica MD04G1222600.v1.1 MD12G1238700.v1.1
prunus_persica Prupe.6G341600_v2.0.a1
pyrus_communis pycom04g19690 pycom12g21940
rosa_chinensis RchiOBHm_Chr3g0451391
rosa_laevigata RLG00000025663
rosa_multiflora Rmu_sc0021369.1_g000001
rosa_roxburghii Rroxscaffold_164G00436530 Rroxscaffold_6G00428810
rosa_rugosa Rorug02G0633500
rosa_samantha Rh3AG036500 Rh3BG037100 Rh3CG035700 Rh3DG036600
rosa_wichuraiana Rw3G002690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 167
AcuI CTGAAG 1 cut(s) 261
AgsI TTSAA 8 cut(s) 77, 151, 203, 276, 376, 392, 458, 511
AluBI AGCT 2 cut(s) 178, 405
AluI AGCT 2 cut(s) 178, 405
Alw21I GWGCWC 1 cut(s) 288
ApeKI GCWGC 2 cut(s) 175, 402
AspS9I GGNCC 2 cut(s) 236, 251
AvaII GGWCC 2 cut(s) 236, 251
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 187, 414
BccI CCATC 1 cut(s) 137
BisI GCNGC 2 cut(s) 176, 403
BlsI GCNGC 2 cut(s) 177, 404
Bme18I GGWCC 2 cut(s) 236, 251
BmgT120I GGNCC 2 cut(s) 236, 251
BmsI GCATC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 18, 365
Bse3DI GCAATG 1 cut(s) 307
BseGI GGATG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 307
BseNI ACTGG 2 cut(s) 18, 365
BseXI GCAGC 2 cut(s) 187, 414
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 1 cut(s) 435
BsmI GAATGC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 288
BspACI CCGC 2 cut(s) 8, 167
BsrDI GCAATG 1 cut(s) 307
BsrI ACTGG 2 cut(s) 18, 365
Bst4CI ACNGT 3 cut(s) 262, 412, 484
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 187, 414
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 25, 529
Cfr13I GGNCC 2 cut(s) 236, 251
CviAII CATG 2 cut(s) 289, 499
CviJI RGCY 6 cut(s) 17, 36, 178, 405, 438, 507
CviKI_1 RGCY 6 cut(s) 17, 36, 178, 405, 438, 507
DdeI CTNAG 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 236, 251
Eco57I CTGAAG 1 cut(s) 261
EcoO109I RGGNCCY 2 cut(s) 236, 251
FaeI CATG 2 cut(s) 292, 502
FaiI YATR 6 cut(s) 59, 132, 191, 290, 381, 500
FatI CATG 2 cut(s) 288, 498
Fnu4HI GCNGC 2 cut(s) 176, 403
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 176, 403
GluI GCNGC 2 cut(s) 176, 403
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 1 cut(s) 176
HapII CCGG 1 cut(s) 435
Hin1II CATG 2 cut(s) 292, 502
HinfI GANTC 3 cut(s) 11, 151, 294
HpaII CCGG 1 cut(s) 435
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 155
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 242, 496
HpyCH4III ACNGT 3 cut(s) 262, 412, 484
HpyCH4V TGCA 2 cut(s) 23, 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 2 cut(s) 292, 502
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 9 cut(s) 31, 54, 110, 140, 252, 330, 357, 378, 448
Lsp1109I GCAGC 2 cut(s) 187, 414
LweI GCATC 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 323, 445
MboII GAAGA 4 cut(s) 149, 191, 254, 523
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 1 cut(s) 516
MnlI CCTC 4 cut(s) 92, 106, 415, 514
MseI TTAA 1 cut(s) 210
MspI CCGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 14
NlaIII CATG 2 cut(s) 292, 502
NmuCI GTSAC 2 cut(s) 323, 445
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 3 cut(s) 11, 151, 294
PkrI GCNGC 2 cut(s) 177, 404
PpuMI RGGWCCY 2 cut(s) 236, 251
Psp5II RGGWCCY 2 cut(s) 236, 251
PspFI CCCAGC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 236, 251
PspPPI RGGWCCY 2 cut(s) 236, 251
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 2 cut(s) 176, 403
Sau96I GGNCC 2 cut(s) 236, 251
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 98, 180, 196, 241, 253, 407
SfaNI GCATC 1 cut(s) 154
SinI GGWCC 2 cut(s) 236, 251
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 516
SsiI CCGC 2 cut(s) 8, 167
TaaI ACNGT 3 cut(s) 262, 412, 484
TaqI TCGA 1 cut(s) 420
TasI AATT 1 cut(s) 516
TfiI GAWTC 3 cut(s) 11, 151, 294
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 2 cut(s) 323, 445
TseI GCWGC 2 cut(s) 175, 402
Tsp45I GTSAC 2 cut(s) 323, 445
TspDTI ATGAA 3 cut(s) 46, 261, 515
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 236, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.