MD04G1224900.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009239
Physical Location & Seq
Reverse (-)
30465611 .. 30473788
8178 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1224900.v1.1.491

Sequence Viewer

Length: 5436 bp
ATGGCGTCGGAGCTAGGGCAACAGACGGTGGAGTTCTCCGCCCTGGTGAGCCGCGCCGCCGAGGAATCTTTCCTCGCGTTGAAAGAGCTCACCGAGAAATCGAAGGCGGCGCCGGACCAGTCCGATACCGATAAGAAAATTGGACTGCTCAAGTACTTGGCCAAGACCCAGCAACGCATGCTTAGGCTCAATGTCCTCGCCAAGTGGTGCCAACAGGTCCCGTTAATACAATACTGCCAGCAACTTTCCTCCACCCTGTCAAGTCATGATACCTGTTTCACTCAAGCAGCAGATTCACTATTTTTCATGCATGACGGGCTGCAGCAAGCTTGTGCTCCTGTTTATGATGTCCCATCTGCAATTGAAATCCTCCTAACAGGGTCATATCAGCGTTTGCCGAAATGTGTAGAAGATGTGGGCATCCAGAGTTCACTAAATGAGGAACAGCAAAAGCCGGCTTTGAAAAAACTGGACACACTTGTGCGATCTAAATTACTAGAAGTATCACTTCCAAAAGAAATTACTGATGTTAAAGTCTCCGATGGTACAGCAGTGATTCGTGTTGATGGAGAATTCAAGGTTTTGGTGACTCTAGGCTACCGAGGGCACCTATCAATGTGGAGGATACTGCATTTGGATCTGCTTGTTGGTGAGAAAAGTGGACCTGTAAAGCTTGAAGTATCGCGACGTCATCTTCTTGGGGATGATTTAGAGCGCAGAATGGCTGATGCAGAAGATCCGTTCATGATACTATATTCAGTTCTGCATGAGCTTTGTGTTGCACTTATCATGGGTACTGTCACAAGGCAAGTACAAGCTCTTCGGCAAGGGAGATGGAAAGACGCAATTCGATTTGAGCTTATATCTGATGGGAGCATGGGTCATGCAGGGACTTCTGCCTCTGCGCAATTAAATCAAGATGGAGAAACTGATTCATCTGGTCTACGAACGCCAGGGTTGAAAATTTTATACTGGCTAGATTTTGATAAAAACAATGGTATTTCTGGTTCAGGATCATGTCCGTCTATAAAGATTGAACCAGGACCGGATCTGCAGATAAAGTGTCTTCATAACACATTTGTCATAGATCCATTAACTGGAAAGGAGGCAGAATTTTCTCTTGACCAAAACTGTATTGATGTTGAGAAGTTGCTTCTGAGAGCCATCTGCTGCAATAGATATACTCGTCTACTTGAAATTCAGAAAGAGCTCGTGAAGAATGTTCAAATCTGCCGAGGTGCAGGGGATGTTTCTCTTCAGTCTCATGTGGAAGAAGTGGAAGCGGACCATAAAAAGAAAGATGATAAGTCCAATGCTGGAGAGTATGAAGGGCAGGAAGTATTACGTGTGCGTGCCTATGGCTCATCATTTTTCACCCTGGGAATAAATTTAAGGAATGGGCGATTTCTTCTTCAGTCCTCTCGTAATATTCTTGCTTCTTCGGGAGTTTTGTCCGAATGTGAAGATGCTTTAAATCAAGGAAGTCTGACTGCAGCTGAAGTTTTTATAAGCTTGAGAAGCAAAAGTATTTTGCACTTATTTGCATCGACTGGCAGGTTTTTAGGCCTTGAGGTGTATGAACATGGTTTTCCTGCAGTTAAAATACCAAAGAACATTTTGAATGGTTCAACTATGTTGCTAATGGGGTTTCCTGATTGCAGTACCTCTTATTTCCTGCTGATGCAACTTGATAAGGACTTTAAACCCCTATTTAAGTTGCTTGAAACTCATCCGGATCCGACTAGAAAACCTGATTCTTTTAATGACCTAAATCATGTGATGCGGATCAAGAAAATTGATGTTAGCCAGATGCAGATGCATGAAGATGATATGAATTTAAGTTTGCTTGACTTGGGAAAACTACATTCCTTTCTGCCCAGTTCCAGGGGTTCTAATCAGTCTGCTGAAAATGGTCTTCTTTCAGAAATCAGCCATGAGGGTTCTATGCCTATTGCTGGGTGTCCACCATCAAGTTTTTCTTCTGTTGTAGACGAAGTGTTTGAACTGGAGAAGGGGTTATCTGTACCTCCTTTTTCTGTTCCTGCTTCTCATTTTGGCTCTGTTCCGATGAATCGTCCCTCTCCTAAGTGGGAAGGAGGTGCGCAGATACCACAGTTAAACAATTCTTCAAAGCTTTCTAGTATGGCCACCCACTATAATGGATCGTTATATCCATCAAATAATTTGAAAAGCCCCGTGCATTCTACTTCTCTTGGTAATCTATCTTCTGGCCCAGGAAGGAGTGCCTCTGTGAAGAAAATACCAGTGTCAAAGTCTGATCAGGATTTGGCTTCTCTTAGGTCTCCACAGTCAGTTGAGTATGGTTCTGGTACTTCAATGGACGAAGATCAGCTGAGATTTCTGAATGAAACTCCAAAGAGTGCAATTTATGGGAGTAAATCATCCCGGCTGTTATCTCCAACCCGGTCTACTGGCCCCAGAGTTTCTGGGCCAGGTGTGAGACCCAACGGGTCCAAAAGTTCACCTAATGGGCCTTTAACTGGACCTTTCAGAGTTTCCGTATCAACATGTGCAACAACTCCCGTATCCCAGGCCCCAGATTCTGGAGTATGTCATAGCCCTAATCTTGATGTTGCGAAAAATGATAGAAAACCTCGCAAACGCACACTTTCAGATATGTTGAATTTGATCCCATCACTTCAGGGTTTCGAAGCTGATTCAGGAGTTCTTAAAAAGAGGAAAACTTCTGAAGTAACTCGTCCTCAGCAGTCTTCCTCACAGGTGCTTATGTCGAGGGATATAATTTCAAATTTTGAAGTATACAATTACGGGGATCTTATATCTGAAGCAAACAGGGGAAATGCACCTTCCAGCATTTATGTTTCAGCCCTTCTTCATGTGATCAGGCACTGTTCACTTTGTATTAAGCATGCCAGATTAACTAGCCAGATGGCGGCTCTGGACATCCCTTATGTTGAAGAAGTGGGTCTAAGAAGTACATCATCAAACATATGGTTTCGAATTCCATTTGCCAGAGGTGATTCATGGCAACACTTATGCTTGCGACTTGGCAGACCTGGAAGCATTTATTGGGATGTCAAAATAAATGACCAGCATTTCAGGGATTTATGGGAGCTTCAAAAAGGAAGCAATAGTACACCTTGGGGTACTGGTGTTCGAATTGCCAATACGTCTGACATAGACTCTCATGTTCGCTATGATCCAGAAGGTGTTGTTCTTTGTTACCAGTCTGTGGAGGCTGATAGTATTAAGAAGTTAGTGGCAGACATCCAAAGACTTTCTAATGCCAGAATGTTTGCTCTTGGGATGCGGAAATTGCTTGGTGTACGAGCAGATGATAAGCCAGAAGAAAGTAGCACAAACCCTGATTTTAAATCACCAGGAGTCAAAGGTTCTCAAGAGGCAACCGATAGACTATCTGAGCAAATGCGAAGGGCATTTAGAATTGAGGCTGTTGGACTTATGAGTCTGTGGTTCAGTTTTGGATCAGGTGTCCTTGCTCGCTTTGTTGTAGAGTGGGAATCAGGTAAAGAGGGTTGTACTATGCATGTATCCCCCGACCAACTTTGGCCCCATACCAAGTTTCTGGAAGATTTCATAAATGGAGCTGAAGTTGCATCACTTTTGGATTGCATTCGACTCACTGCAGGGCCCTTACATGCTCTTGCAGCCGCAACACGACCTGCACGAGCTAGTCCTATCCCGGGGGTCCCTGGTGGAGCAGTTCTCTCTTCTATCCCAAAACAGGCTGGGTACTTATCATCTCAGGGCCTTATGCCAACTAGTTCAACCACAAATGCTGGTCAGTCTCCTGGCCCTATGGGGAACCCAGTTTCATCTCCTGCTACAGGGGCTCTTGCAAATCATAGCCTCCATGGGCCTGCTGGGTTAGCTGGTGCTGGCCGAGGTGGACCTGGCATTGTACCCAGCTCACTGTTGCCCATTGATGTCTCTGTTGTGCTACGTGGACCCTATTGGATACGGATCATATACCGAAAGAATTTCGCAGTTGACATGCGCTGTTTTGCAGGAGATCAGGTTTGGTTGCAACCAGCAACGCCACCCTATGGGGGGCCTTCAATTGGAGGGTCATTACCTTGCCCTCAGTTTAGACCATTTATTATGGAGCATGTAGCCCAAGAATTGAATGGTTTAGATACTAATTTCACCGGTGGTCAACAGACAGGACTGTCAAGTTCAATCAATCAAAACCCCAGTTCAGGTTCCCAACTGTCAACTGTAAATGGAAATAGGGTTAACCTTCCTAGTTCTGCTGCAATGTCTAGGACAGGCAACAACCAAGTGGCTGGTTTAAACCGTGTCGGAAATGCTTCTCCAGTATCCTCAAATTTGGCTGTTGTGAGCTCAGCAGGGCCGGCTTTACGAAGATCCCCAGGTCCTGGTGCTGCTCATGTGAGAGGAGAACTGAATACAGCCATTATTGGTCTTGGAGATGACGGAGGGTATGGAGGTGGTTGGGTTCCTCTTGTTGCTCTTAAAAAGGTGCTAAGAGGTATTCTCAAGTATCTTGGAGTTCTATGGCTATTCGCACAGCTACCAAATCTTTTGAAAGAGATTTTGGGGTCAATTCTGAAAGACAATGAAGGTGCCCTTCTAAATTTAGACCAGGAGCAGCCCGCCTTGAGATTCTTTGTTGGTGGCTATGTATTTGCAGTAAGTGTTCACAGAGTTCAACTTCTTCTTCAAGTACTGAGCGTGAAACGATTCCATCAGCAGCAGCAACAACAAGGGCAACAGCCAAATACAAACACTGCTAATGAGGAATTATCTACAGCCGAAATAGGAGAAATATGTGACTACTTCAGTCGACGTGTTGCTTCAGAGCCTTACGATGCTTCTCGTGTCGCGTCGTTCATTACTCTCCTCACCTTACCCATATCAGTTTTAAGAGAATTCTTGAAATTAATAGCATGGAAAAAAGCACTAGCCCTGGCACAAGGGGGAGATATAGCACCTGCTCAAAAACCCCGTATTGAATTATGCCTTGAAAACCATGCTGGCTCGAGTATGGATCATTCATCTGTTGCCAAAAGCAATATTCATTATGATCGGCCTCATAACTCGGTTGATTTTGCACTGACTCTTGTTCTTGATCCTGCTCACATACCTCATATAAATGCCGCTGGTGGAGCTGCATGGTTGCCATACTGTGTCTCAGTGAGGTTGAGATATTCGTTTGGTGAAAATCCTAACGTGTCCTTTCTCGGTATGGAAGGAAGCCATGGAGGTCGAGCTTGCTGGTTGCGCGCTGATGACTGGGAGAAGTGCAAACACAAGGTTGCTCGAACTGTGGAACATCATGGTAGCTCAGGAGTAGACAGTGGTCAAGGAAGGTTAAGAATCGTAGCTGACTATGTGCAAAGGACACTGCACATATGGCTTCAAGGGTTGAGGGACGGTAGCGCGGTTAGTGCAACCTCCATGGCAACATGA

Protein Analysis

1812

Amino Acids

196.9

Weight (kDa)

8.23

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med14 PF08638 9 - 199 1e-50 Mediator complex subunit MED14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1508
AarI CACCTGC 1 cut(s) 4966
AasI GACNNNNNNGTC 2 cut(s) 3447, 4171
AatII GACGTC 1 cut(s) 691
Acc16I TGCGCA 2 cut(s) 906, 2103
Acc36I ACCTGC 3 cut(s) 1545, 3673, 4966
AccB1I GGYRCC 4 cut(s) 109, 207, 606, 4589
AccB7I CCANNNNNTGG 5 cut(s) 1097, 2564, 3214, 4049, 4382
AccI GTMKAC 7 cut(s) 943, 1189, 1989, 2429, 2781, 4810, 5319
AccII CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
AccIII TCCGGA 1 cut(s) 1732
AcoI YGGCCR 3 cut(s) 159, 2145, 3883
AcuI CTGAAG 9 cut(s) 1241, 1397, 1518, 2645, 2730, 2826, 3612, 4789, 4806
AcyI GRCGYC 3 cut(s) 5, 110, 688
AflIII ACRYGT 4 cut(s) 1345, 2528, 4813, 5196
AgeI ACCGGT 1 cut(s) 4151
AhlI ACTAGT 1 cut(s) 3764
AjiI CACGTC 1 cut(s) 4814
AjuI GAANNNNNNNTTGG 4 cut(s) 3034, 3066, 4544, 4576
AleI CACNNNNGTG 1 cut(s) 479
Alw21I GWGCWC 4 cut(s) 90, 337, 1212, 4349
Alw26I GTCTC 7 cut(s) 541, 1266, 2307, 2455, 3795, 3937, 5161
AlwNI CAGNNNCTG 3 cut(s) 2564, 2871, 4382
Ama87I CYCGRG 2 cut(s) 3685, 5005
Aor13HI TCCGGA 1 cut(s) 1732
ApaI GGGCCC 1 cut(s) 3636
ArsI GACNNNNNNTTYG 2 cut(s) 1982, 2014
AseI ATTAAT 1 cut(s) 4907
AsiGI ACCGGT 1 cut(s) 4151
Asp700I GAANNNNTTC 2 cut(s) 4312, 4706
AspLEI GCGC 9 cut(s) 56, 112, 717, 907, 2104, 4002, 5250, 5252, 5408
AsuC2I CCSGG 4 cut(s) 2407, 2425, 3686, 3687
AsuII TTCGAA 3 cut(s) 2670, 2980, 3139
AvaI CYCGRG 2 cut(s) 3685, 5005
BaeGI GKGCMC 3 cut(s) 609, 3636, 4594
BaeI ACNNNNGTAYC 8 cut(s) 537, 570, 2099, 2132, 2529, 2562, 3120, 3153
BalI TGGCCA 2 cut(s) 161, 2147
BamHI GGATCC 1 cut(s) 1735
BanI GGYRCC 4 cut(s) 109, 207, 606, 4589
BanII GRGCYC 5 cut(s) 90, 1212, 3636, 3838, 4349
BarI GAAGNNNNNNTAC 6 cut(s) 804, 836, 2209, 2241, 3100, 3132
BauI CACGAG 3 cut(s) 1211, 3669, 4842
BbsI GAAGAC 3 cut(s) 1058, 1907, 2724
Bbv12I GWGCWC 4 cut(s) 90, 337, 1212, 4349
BbvCI CCTCAGC 1 cut(s) 2724
BciVI GTATCC 5 cut(s) 618, 2557, 3544, 3954, 4333
BclI TGATCA 2 cut(s) 2278, 2862
BcnI CCSGG 4 cut(s) 2407, 2425, 3686, 3687
BcoDI GTCTC 7 cut(s) 541, 1266, 2307, 2455, 3795, 3937, 5161
BcuI ACTAGT 1 cut(s) 3764
BfmI CTRYAG 7 cut(s) 320, 1052, 1491, 1593, 3627, 3828, 4773
BfoI RGCGCY 1 cut(s) 113
BfuAI ACCTGC 3 cut(s) 1545, 3673, 4966
BfuI GTATCC 5 cut(s) 618, 2557, 3544, 3954, 4333
BlpI GCTNAGC 1 cut(s) 4348
BmcAI AGTACT 2 cut(s) 155, 4692
BmeT110I CYCGRG 2 cut(s) 3685, 5005
BmgBI CACGTC 1 cut(s) 4814
BmrI ACTGGG 4 cut(s) 1872, 3806, 4191, 5269
BmuI ACTGGG 4 cut(s) 1872, 3806, 4191, 5269
BoxI GACNNNNGTC 1 cut(s) 5325
BpiI GAAGAC 3 cut(s) 1058, 1907, 2724
BplI GAGNNNNNCTC 4 cut(s) 3693, 3725, 4485, 4517
BpmI CTGGAG 4 cut(s) 1338, 2027, 2586, 4302
Bpu10I CCTNAGC 3 cut(s) 182, 2724, 5311
Bpu1102I GCTNAGC 1 cut(s) 4348
Bpu14I TTCGAA 3 cut(s) 2670, 2980, 3139
BpuEI CTTGAG 7 cut(s) 134, 267, 1534, 1589, 3363, 4487, 4645
BpuMI CCSGG 4 cut(s) 2407, 2425, 3686, 3687
BsaAI YACGTR 2 cut(s) 1346, 3947
BsaBI GATNNNNATC 2 cut(s) 1784, 3965
BsaHI GRCGYC 3 cut(s) 5, 110, 688
BsaI GGTCTC 2 cut(s) 2307, 2455
BsaWI WCCGGW 3 cut(s) 1045, 1732, 4151
BsaXI ACNNNNNCTCC 8 cut(s) 865, 895, 2000, 2030, 3579, 3609, 4005, 4035
Bse118I RCCGGY 3 cut(s) 454, 4151, 4357
Bse3DI GCAATG 1 cut(s) 4266
Bse8I GATNNNNATC 2 cut(s) 1784, 3965
BseAI TCCGGA 1 cut(s) 1732
BseGI GGATG 9 cut(s) 420, 709, 1252, 1729, 2402, 2925, 3061, 3249, 3294
BseJI GATNNNNATC 2 cut(s) 1784, 3965
BseMI GCAATG 1 cut(s) 4266
BsePI GCGCGC 1 cut(s) 5248
BseRI GAGGAG 2 cut(s) 4416, 4856
BseSI GKGCMC 3 cut(s) 609, 3636, 4594
BseYI CCCAGC 5 cut(s) 168, 1955, 3731, 3866, 3908
BsgI GTGCAG 3 cut(s) 1260, 3651, 5357
Bsh1236I CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
BshNI GGYRCC 4 cut(s) 109, 207, 606, 4589
BshTI ACCGGT 1 cut(s) 4151
BsiHKAI GWGCWC 4 cut(s) 90, 337, 1212, 4349
BsiHKCI CYCGRG 2 cut(s) 3685, 5005
BsiSI CCGG 9 cut(s) 113, 455, 1046, 1733, 2407, 2425, 3686, 4152, 4358
BslFI GGGAC 6 cut(s) 203, 335, 904, 2061, 3677, 5411
BsmAI GTCTC 7 cut(s) 541, 1266, 2307, 2455, 3795, 3937, 5161
BsmFI GGGAC 6 cut(s) 203, 335, 904, 2061, 3677, 5411
BsmI GAATGC 2 cut(s) 2200, 3615
Bso31I GGTCTC 2 cut(s) 2307, 2455
BsoBI CYCGRG 2 cut(s) 3685, 5005
Bsp119I TTCGAA 3 cut(s) 2670, 2980, 3139
Bsp120I GGGCCC 1 cut(s) 3632
Bsp1286I GDGCHC 8 cut(s) 90, 337, 609, 1212, 3636, 3838, 4349, 4594
Bsp13I TCCGGA 1 cut(s) 1732
Bsp1720I GCTNAGC 1 cut(s) 4348
Bsp19I CCATGG 3 cut(s) 3856, 5224, 5424
Bsp68I TCGCGA 1 cut(s) 685
BspEI TCCGGA 1 cut(s) 1732
BspFNI CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
BspHI TCATGA 2 cut(s) 265, 744
BspMAI CTGCAG 5 cut(s) 324, 1056, 1495, 1597, 3631
BspMI ACCTGC 3 cut(s) 1545, 3673, 4966
BspQI GCTCTTC 1 cut(s) 825
BspT104I TTCGAA 3 cut(s) 2670, 2980, 3139
BspT107I GGYRCC 4 cut(s) 109, 207, 606, 4589
BspTNI GGTCTC 2 cut(s) 2307, 2455
BsrDI GCAATG 1 cut(s) 4266
BsrFI RCCGGY 3 cut(s) 454, 4151, 4357
BssAI RCCGGY 3 cut(s) 454, 4151, 4357
BssHII GCGCGC 1 cut(s) 5248
BssNAI GTATAC 1 cut(s) 2782
BssNI GRCGYC 3 cut(s) 5, 110, 688
BssSI CACGAG 3 cut(s) 1211, 3669, 4842
BssT1I CCWWGG 4 cut(s) 3122, 3856, 5224, 5424
Bst1107I GTATAC 1 cut(s) 2782
Bst2BI CACGAG 3 cut(s) 1211, 3669, 4842
Bst6I CTCTTC 3 cut(s) 825, 1260, 3718
BstACI GRCGYC 3 cut(s) 5, 110, 688
BstAPI GCANNNNNTGC 1 cut(s) 178
BstBAI YACGTR 2 cut(s) 1346, 3947
BstBI TTCGAA 3 cut(s) 2670, 2980, 3139
BstDSI CCRYGG 3 cut(s) 3856, 5224, 5424
BstENI CCTNNNNNAGG 1 cut(s) 3828
BstF5I GGATG 9 cut(s) 420, 709, 1252, 1729, 2402, 2925, 3061, 3249, 3294
BstFNI CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 9 cut(s) 56, 112, 717, 907, 2104, 4002, 5250, 5252, 5408
BstMAI GTCTC 7 cut(s) 541, 1266, 2307, 2455, 3795, 3937, 5161
BstNSI RCATGY 7 cut(s) 181, 2532, 2894, 3533, 3644, 4000, 4115
BstPAI GACNNNNGTC 1 cut(s) 5325
BstSFI CTRYAG 7 cut(s) 320, 1052, 1491, 1593, 3627, 3828, 4773
BstSLI GKGCMC 3 cut(s) 609, 3636, 4594
BstUI CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
BstV2I GAAGAC 3 cut(s) 1058, 1907, 2724
BstX2I RGATCY 7 cut(s) 637, 736, 1048, 1087, 1735, 2794, 4370
BstXI CCANNNNNNTGG 3 cut(s) 2159, 3568, 4289
BstYI RGATCY 7 cut(s) 637, 736, 1048, 1087, 1735, 2794, 4370
BstZ17I GTATAC 1 cut(s) 2782
BsuI GTATCC 5 cut(s) 618, 2557, 3544, 3954, 4333
BtgI CCRYGG 3 cut(s) 3856, 5224, 5424
BtrI CACGTC 1 cut(s) 4814
BtsCI GGATG 9 cut(s) 420, 709, 1252, 1729, 2402, 2925, 3061, 3249, 3294
BtsI GCAGTG 4 cut(s) 558, 3624, 4752, 5369
BtuMI TCGCGA 1 cut(s) 685
BveI ACCTGC 3 cut(s) 1545, 3673, 4966
CaiI CAGNNNCTG 3 cut(s) 2564, 2871, 4382
CciI TCATGA 2 cut(s) 265, 744
CfoI GCGC 9 cut(s) 56, 112, 717, 907, 2104, 4002, 5250, 5252, 5408
Cfr10I RCCGGY 3 cut(s) 454, 4151, 4357
Cfr9I CCCGGG 1 cut(s) 3685
CseI GACGC 2 cut(s) 851, 4839
CspAI ACCGGT 1 cut(s) 4151
DinI GGCGCC 1 cut(s) 111
DraI TTTAAA 4 cut(s) 1473, 1702, 3355, 4296
DrdI GACNNNNNNGTC 2 cut(s) 3447, 4171
DseDI GACNNNNNNGTC 2 cut(s) 3447, 4171
EaeI YGGCCR 3 cut(s) 159, 2145, 3883
Eam1104I CTCTTC 3 cut(s) 825, 1260, 3718
EarI CTCTTC 3 cut(s) 825, 1260, 3718
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 3 cut(s) 88, 1210, 4347
Eco130I CCWWGG 4 cut(s) 3122, 3856, 5224, 5424
Eco147I AGGCCT 1 cut(s) 1566
Eco24I GRGCYC 5 cut(s) 90, 1212, 3636, 3838, 4349
Eco31I GGTCTC 2 cut(s) 2307, 2455
Eco53kI GAGCTC 3 cut(s) 88, 1210, 4347
Eco57I CTGAAG 9 cut(s) 1241, 1397, 1518, 2645, 2730, 2826, 3612, 4789, 4806
Eco88I CYCGRG 2 cut(s) 3685, 5005
EcoICRI GAGCTC 3 cut(s) 88, 1210, 4347
EcoNI CCTNNNNNAGG 1 cut(s) 3828
EcoO109I RGGNCCY 8 cut(s) 217, 2554, 3632, 3633, 3691, 3751, 4055, 4379
EcoRI GAATTC 3 cut(s) 572, 2982, 4895
EcoT14I CCWWGG 4 cut(s) 3122, 3856, 5224, 5424
EcoT22I ATGCAT 3 cut(s) 312, 1821, 3531
EcoT38I GRGCYC 5 cut(s) 90, 1212, 3636, 3838, 4349
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 4 cut(s) 3122, 3856, 5224, 5424
FalI AAGNNNNNCTT 6 cut(s) 492, 524, 1963, 1995, 4578, 4610
FaqI GGGAC 6 cut(s) 203, 335, 904, 2061, 3677, 5411
FauI CCCGC 1 cut(s) 4627
FauNDI CATATG 2 cut(s) 2972, 5378
FbaI TGATCA 2 cut(s) 2278, 2862
FblI GTMKAC 7 cut(s) 943, 1189, 1989, 2429, 2781, 4810, 5319
FokI GGATG 9 cut(s) 407, 716, 1259, 1716, 2389, 2912, 3068, 3236, 3301
FriOI GRGCYC 5 cut(s) 90, 1212, 3636, 3838, 4349
FspI TGCGCA 2 cut(s) 906, 2103
GlaI GCGC 9 cut(s) 55, 111, 716, 906, 2103, 4001, 5249, 5251, 5407
GsaI CCCAGC 5 cut(s) 172, 1959, 3735, 3870, 3912
GsuI CTGGAG 4 cut(s) 1338, 2027, 2586, 4302
HaeII RGCGCY 1 cut(s) 113
HapII CCGG 9 cut(s) 113, 455, 1046, 1733, 2407, 2425, 3686, 4152, 4358
HgaI GACGC 2 cut(s) 851, 4839
HhaI GCGC 9 cut(s) 56, 112, 717, 907, 2104, 4002, 5250, 5252, 5408
Hin1I GRCGYC 3 cut(s) 5, 110, 688
Hin6I GCGC 9 cut(s) 54, 110, 715, 905, 2102, 4000, 5248, 5250, 5406
HinP1I GCGC 9 cut(s) 54, 110, 715, 905, 2102, 4000, 5248, 5250, 5406
HincII GTYRAC 5 cut(s) 3994, 4160, 4218, 4240, 4811
HindII GTYRAC 5 cut(s) 3994, 4160, 4218, 4240, 4811
HindIII AAGCTT 4 cut(s) 327, 671, 1510, 2132
HpaI GTTAAC 1 cut(s) 4240
HpaII CCGG 9 cut(s) 113, 455, 1046, 1733, 2407, 2425, 3686, 4152, 4358
Hpy99I CGWCG 4 cut(s) 10, 690, 4815, 4855
HpyCH4IV ACGT 6 cut(s) 688, 1345, 3152, 3946, 4813, 5196
HpySE526I ACGT 6 cut(s) 688, 1345, 3152, 3946, 4813, 5196
Hsp92I GRCGYC 3 cut(s) 5, 110, 688
HspAI GCGC 9 cut(s) 54, 110, 715, 905, 2102, 4000, 5248, 5250, 5406
KasI GGCGCC 1 cut(s) 109
KflI GGGWCCC 1 cut(s) 3691
Kpn2I TCCGGA 1 cut(s) 1732
KroI GCCGGC 2 cut(s) 454, 4357
KroNI GCCGGC 2 cut(s) 456, 4359
Ksp22I TGATCA 2 cut(s) 2278, 2862
KspAI GTTAAC 1 cut(s) 4240
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 9 cut(s) 10, 340, 873, 3094, 3587, 3701, 4108, 4612, 5132
MaeII ACGT 6 cut(s) 688, 1345, 3152, 3946, 4813, 5196
MaeIII GTNAC 5 cut(s) 586, 799, 2713, 3203, 4796
MfeI CAATTG 2 cut(s) 360, 4062
MflI RGATCY 7 cut(s) 637, 736, 1048, 1087, 1735, 2794, 4370
MhlI GDGCHC 8 cut(s) 90, 337, 609, 1212, 3636, 3838, 4349, 4594
MlsI TGGCCA 2 cut(s) 161, 2147
MluNI TGGCCA 2 cut(s) 161, 2147
Mly113I GGCGCC 1 cut(s) 110
MlyI GAGTC 6 cut(s) 583, 3158, 3375, 3457, 3615, 5077
MmeI TCCRAC 4 cut(s) 1763, 2444, 3418, 4285
Mox20I TGGCCA 2 cut(s) 161, 2147
Mph1103I ATGCAT 3 cut(s) 312, 1821, 3531
MroI TCCGGA 1 cut(s) 1732
MroNI GCCGGC 2 cut(s) 454, 4357
MroXI GAANNNNTTC 2 cut(s) 4312, 4706
MscI TGGCCA 2 cut(s) 161, 2147
MslI CAYNNNNRTG 4 cut(s) 479, 1824, 2157, 5118
Msp20I TGGCCA 2 cut(s) 161, 2147
MspA1I CMGCKG 3 cut(s) 1496, 2353, 5126
MspI CCGG 9 cut(s) 113, 455, 1046, 1733, 2407, 2425, 3686, 4152, 4358
MssI GTTTAAAC 1 cut(s) 4296
MunI CAATTG 2 cut(s) 360, 4062
Mva1269I GAATGC 2 cut(s) 2200, 3615
MvnI CGCG 6 cut(s) 54, 77, 685, 4850, 5250, 5408
NaeI GCCGGC 2 cut(s) 456, 4359
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 4 cut(s) 2407, 2425, 3686, 3687
NcoI CCATGG 3 cut(s) 3856, 5224, 5424
NdeI CATATG 2 cut(s) 2972, 5378
NgoMIV GCCGGC 2 cut(s) 454, 4357
NmeAIII GCCGAG 3 cut(s) 85, 1259, 3911
NmuCI GTSAC 3 cut(s) 586, 799, 4796
NruI TCGCGA 1 cut(s) 685
NsbI TGCGCA 2 cut(s) 906, 2103
NsiI ATGCAT 3 cut(s) 312, 1821, 3531
NspI RCATGY 7 cut(s) 181, 2532, 2894, 3533, 3644, 4000, 4115
NspV TTCGAA 3 cut(s) 2670, 2980, 3139
OliI CACNNNNGTG 1 cut(s) 479
PaeI GCATGC 2 cut(s) 181, 2894
PaeR7I CTCGAG 1 cut(s) 5005
PagI TCATGA 2 cut(s) 265, 744
PaqCI CACCTGC 1 cut(s) 4966
PasI CCCWGGG 1 cut(s) 1378
PauI GCGCGC 1 cut(s) 5248
PceI AGGCCT 1 cut(s) 1566
PciI ACATGT 1 cut(s) 2528
PciSI GCTCTTC 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 3667
PctI GAATGC 2 cut(s) 2200, 3615
PdiI GCCGGC 2 cut(s) 456, 4359
PdmI GAANNNNTTC 2 cut(s) 4312, 4706
PflMI CCANNNNNTGG 5 cut(s) 1097, 2564, 3214, 4049, 4382
PinAI ACCGGT 1 cut(s) 4151
PleI GAGTC 6 cut(s) 583, 3158, 3374, 3456, 3615, 5077
PluTI GGCGCC 1 cut(s) 113
PmeI GTTTAAAC 1 cut(s) 4296
PpsI GAGTC 6 cut(s) 583, 3158, 3374, 3456, 3615, 5077
Ppu21I YACGTR 2 cut(s) 1346, 3947
PpuMI RGGWCCY 3 cut(s) 217, 3691, 4379
PscI ACATGT 1 cut(s) 2528
PshAI GACNNNNGTC 1 cut(s) 5325
PshBI ATTAAT 1 cut(s) 4907
PsiI TTATAA 1 cut(s) 1508
Psp124BI GAGCTC 3 cut(s) 90, 1212, 4349
Psp5II RGGWCCY 3 cut(s) 217, 3691, 4379
PspFI CCCAGC 5 cut(s) 168, 1955, 3731, 3866, 3908
PspOMI GGGCCC 1 cut(s) 3632
PspPPI RGGWCCY 3 cut(s) 217, 3691, 4379
PspXI VCTCGAGB 1 cut(s) 5005
PsrI GAACNNNNNNTAC 2 cut(s) 991, 1023
PstI CTGCAG 5 cut(s) 324, 1056, 1495, 1597, 3631
PstNI CAGNNNCTG 3 cut(s) 2564, 2871, 4382
PsuI RGATCY 7 cut(s) 637, 736, 1048, 1087, 1735, 2794, 4370
PteI GCGCGC 1 cut(s) 5248
PvuII CAGCTG 2 cut(s) 1496, 2353
RruI TCGCGA 1 cut(s) 685
RseI CAYNNNNRTG 4 cut(s) 479, 1824, 2157, 5118
SacI GAGCTC 3 cut(s) 90, 1212, 4349
SalI GTCGAC 1 cut(s) 4809
SapI GCTCTTC 1 cut(s) 825
ScaI AGTACT 2 cut(s) 155, 4692
SchI GAGTC 6 cut(s) 583, 3158, 3375, 3457, 3615, 5077
SduI GDGCHC 8 cut(s) 90, 337, 609, 1212, 3636, 3838, 4349, 4594
SfcI CTRYAG 7 cut(s) 320, 1052, 1491, 1593, 3627, 3828, 4773
SfoI GGCGCC 1 cut(s) 111
Sfr274I CTCGAG 1 cut(s) 5005
SfuI TTCGAA 3 cut(s) 2670, 2980, 3139
SgrAI CRCCGGYG 1 cut(s) 4151
SlaI CTCGAG 1 cut(s) 5005
SmaI CCCGGG 1 cut(s) 3687
SmiMI CAYNNNNRTG 4 cut(s) 479, 1824, 2157, 5118
SmlI CTYRAG 8 cut(s) 149, 282, 1513, 1568, 3378, 4502, 4624, 5005
SmoI CTYRAG 8 cut(s) 149, 282, 1513, 1568, 3378, 4502, 4624, 5005
SpeI ACTAGT 1 cut(s) 3764
SphI GCATGC 2 cut(s) 181, 2894
SseBI AGGCCT 1 cut(s) 1566
SspDI GGCGCC 1 cut(s) 109
SspI AATATT 2 cut(s) 1429, 5041
SstI GAGCTC 3 cut(s) 90, 1212, 4349
StuI AGGCCT 1 cut(s) 1566
StyI CCWWGG 4 cut(s) 3122, 3856, 5224, 5424
TaiI ACGT 6 cut(s) 691, 1348, 3155, 3949, 4816, 5199
TatI WGTACW 6 cut(s) 153, 811, 2957, 3116, 3521, 4690
TauI GCSGC 6 cut(s) 54, 59, 110, 2918, 3656, 5126
TseFI GTSAC 3 cut(s) 586, 799, 4796
Tsp45I GTSAC 3 cut(s) 586, 799, 4796
TspGWI ACGGA 5 cut(s) 729, 1011, 2509, 3979, 4455
TspMI CCCGGG 1 cut(s) 3685
Van91I CCANNNNNTGG 5 cut(s) 1097, 2564, 3214, 4049, 4382
VspI ATTAAT 1 cut(s) 4907
XagI CCTNNNNNAGG 1 cut(s) 3828
XceI RCATGY 7 cut(s) 181, 2532, 2894, 3533, 3644, 4000, 4115
XcmI CCANNNNNNNNNTGG 2 cut(s) 3863, 4127
XhoI CTCGAG 1 cut(s) 5005
XmaI CCCGGG 1 cut(s) 3685
XmiI GTMKAC 7 cut(s) 943, 1189, 1989, 2429, 2781, 4810, 5319
XmnI GAANNNNTTC 2 cut(s) 4312, 4706
ZraI GACGTC 1 cut(s) 689
ZrmI AGTACT 2 cut(s) 155, 4692
Zsp2I ATGCAT 3 cut(s) 312, 1821, 3531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.