Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009239

Physical Location & Seq

Genomic Coordinates Reverse (-)

31325378 .. 31333452

Gene Structure 8075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1241600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5409 bp

ATGGCGTCGGAGCTAGGGCAACAGACGGTGGAGTTCTCCGCCCTGGTGAGCCGCGCCGCCGAGGAATCCTTCCTCGCGCTGAAAGAGCTCACCGAGAAATCGAAGGGGGCGCCGGACCAGTCCGATACCGATAAGAAAATTGGACTGCTCAAGTACTTGGCTAAGACCCAGCAACGCATGCTCCGGCTCAATGTCCTCGCCAAGTGGTGCCAACAGGTCCCGTTAATACAATACTGCCAGCAACTTACCTCCACTCTATCAAGTCATGATACCTGTTTCACTCAAGCAGCAGATTCACTATTTTTCATGCATGATGGGCTGCAGCAAGCTTGTGCTCCTGTTTATGATGTTCCATCTGCAATTGAAATCCTCCTAACAGGGTCATATCAGCGTTTGCCGAAATGTGTAGAAGACGTGGGCATCCAGAGCTCACTAAATGAGGAACAGCAGAAGCCGGCTTTGAAAAAGCTGGACACACTTGTGCGATCTAAATTACTGGAAGTATCACTTCCAAAAGAAATTACTGATGTTAAAGTCTCCGATGGTACAGCAGTGCTTCGTGTTGATGGAGAATTCAAGGTTTTGGTGACTCTAGGCTACCGAGGGCACCTATCAATGTGGAGGATACTGCATTTGGAACTGCTTGTTGGTGAGAGAAGTGGACCTGTAAAGCTTGAAGTATCGCGGCGGCATCTTCTTGGGGATGATTTAGAGCGCAGAATGGCTGCTACAGAAAATCCGTTCATGATATTATACTCAGTTCTACATGAGCTTTGTGTTGCACTTATCATGGGCACTGTCACAAGGCAAGTACAAGCTCTTCGGCAAGGTAGATGGAAAGATGCAATTCGATTTGAGCTCATATCCGATGGGAGCATGAGTCATGCAGGGACTTCTGCCTCTGCACAATTAAATCAAGATGGAGAAACTGATTCATCTGGTCTACGAACACCAGGGTTAAAATTTTTATACTGGTTAGATTTTGATAAAAACAATGGTATTTCTGATTCAGCATCATGTCCGTCTATAAAAATTGAACCAGGACCGGATCTGCAGATAAAGTGTCTTCATAGCACATTTGTCATAGATCCATTAACTGGAAAGGAGGCAGAATTTTCTCTTGACCAAAACTGTATTGATGTTGAGAGGTTGCTTCTGAGAGCCATCTGCTGTAACAGATATACTCGTCTGCTTGAAATTCAGAAAGAGCTGGTGAAAAATGTTCAAATCTGGCGAGGTGCAGGGGATGTTTCTCTTCAGTCTCATGTGGAAGAAGCGGATGTTGACCATAAAAAGAAAGAAGATAAGTCCAATGCTGGAGAATATGAAGGGCAGGAAGTATTACGTGTGCGCGCCTATGGCTCATCATTTTTCACCCTGGGAATAAACATTAGGAATGGGCGATTTCGTCTTCAGTCCTCTCATAATATTCTTGCTCCTTCGGGAGTTTTGTCTGAATGTGAAGATACTTTAAATCAAGGAAGTATGACTGCAGCTGAAGTTTTCATAAATTTGAGAAGCAGAAGTATTTTGCACTTATTTGCATCTACTGGCCGATTTCTAGGGCTTGAGGTGTATGAACATAGTTTTCCTGCAGTTAAAATACCGAAGAACATTTTGAATGGTTCAACTATGTTGCTAATGGGGTTTCCTGATTGCGGGAGCTCTTATTTCTTGCTGATGCAACTTGATAAGGACTTTAAACCCCTATTTAAATTGCTTGAAACTCAGCCAGATCCATCTAGAAAACCCGATTCTTTAAATGACTTAAACCAAGTGATGCGGATCAAGAAAATTGATGTTAGCCAGATGCAGATGCATGAAGATGATATGAATTTAAGCTTGCTTGACTTGGGAAAACTACAATCCTTTCTGCCCAGTTCCAGGGGTTCTAATCAGTCTTCTGAAAATGGTTTTCTTTCGGATATCAGCAATGAAGGTTCTATGCCTATTGCAGGGTGTCCACCTTCGAGTTTTTCTTCTGTTGTAGATGAAGTGTTTGAACTAGAGAAGGGGTTATCTGTACTTCCTTTTTCTGTTCCTGGTTCTCATTTTGGATCTGCTCCGATGAATTGTCCCTCTCCTAAATGGGAAGGAGTTATGCAGATATCACAGTTAAATAATTCTTCAAATCTTTCTAGCATGGCCACCCACTATAATGGATCCTTATATCCATCAAATAATTTGAAAGGCCCCGTACATTCTGCTTCTCTTGGTAATCTACCTTCTGGCCCAGGAAGGAGTGCCACTGTGAGGAAAATACCAGTGTCAAAATCTGATCAGGATTTGGCTTCTCTTAGGTCTCCACAGTCAGTTGAGTATGGTTCTGGTACTTCAGTGGATGAAGATCAGCTGAGATTTATGAATGAAACTTCAAAGGGTGCAATATATGGGAATAAATCATCCCGGTTATTATCTCCACCTCGGTCTACTGGTCCCAGAATTTCTGGGCCTGGTGTGAGACCCAACGGGCCCAAAAGTACACCTATCGGGCCTTTAACTGGACCTTCCAGAGTTGCCGGATCAAACTCATATGCAACCACTCCCGTATCCCAGGTCCCAGATTCGGGAGTATGTCATAGCCCTAACCATGATGTTTCGAAAAATGATAGAAAACCTCGCAAACGCACAGTTTCAGATATGTTGAATTTGATCCCATCACTTCAGGGTGTGGAAGCCGATTCAGGAGTTTTTAAAAGGAGGAAAACTTCTGAAGTAACTCATCCTCATCAGTCTTCTTCACAGATGCTTATGTCAAGGGATATAATTTCAAAATTTGAAGTAAACAGTTTCGGGGATCTGATATCTGAAGCAAATAAGGGAAATGCACCTTCCAGCATTTATGTTTCAGCTCTTCTTCATGTGATCAGACACTGTTCTCTTTGTATTAAGCATGCCAGATTAACTAGCCAGATGGCGGCTCTGGATATCCCTTATGTTGAAGAAGTGGGTCTAAGAAGTACATCATCAAACATATGGTTTCGACTTCCATTTGCCAGAGGTGATGCATGGCAACACTTATGCTTGCGACTTGGCAGACCTGGAAGCATTTATTGGGATGTCAAAATAAATGACCAGCATTTCAGGGATTTGTGGGAGCTTCAGAAAGGAAGCAATGGTACACCTTGGGGTTCTGGTGTTCGAATTGCCAATACATCTGACATTGACTCTCATATTCGCTATGATCCAGAGGGTGTTGTTCTTAGTTATCAGTCTGTGGAGGCTGATAGTATTAAGAAGTTAGTGGCTGACATCCAAAGACTTTCTAATGCAAGAATGTTTGCCCTTGGGATGCGGAGATTGCTTGGTGTAAGAGCAGATGAGAAGCCAGAAGAAAGTAGTACAAACCCTGATTTTAAAGCACTAGGAGTCAAAGTTTCTCCTGAGGCAACTGATAGACTATCTGAGCAAATGCGAAGGGCATTTAGAATTGAGGCTGTTGGACTTATGAGTCTGTGGTTCAGTTTTGGATCAGGTGTCCTTGCTCGCTTTGTTGTAGAGTGGGAGTCAGGTAAAGAGGGTTGTACTATGCATGTAACCCCCGACCAACTTTGGCCCCATACCAAGTTCCTGGAAGATTTCATAAATGGAGCTGAAGTTGCTTCACTTTTGGATTGCATTCGACTCACTGCAGGGCCCTTGCATGCTCTTGCAGCGGCAACACGACCTGCACGAGCTAGTCCTATACCGGGGGTCCCTGGAGGAGCAGTCCTCTCTTCTATCCCAAAGCAGGCTGGGTACTTACCATCTCAGGGCCTTATGCCTACTAGTTCAACCACAAATGCCAGTCAGTCTCCTGGCCTTATGGGGAACGCAGTTTCATCTCCTTCTACAGGGCCTCTTGCAAATCATAGCCCGCATGGGGCTGCTGGTTTAGCTGGTGCTGGCCGAGGTGGACCTGGCATTGTACCCAGCTCACTGTTGCCCATTGATGTCTCTGTTGTGCTACGTGGACCCTATTGGATACGGATCATATACCGAAAGCACTTCGCGGTTGACATGCGCTGTTTTGCAGGAGATCAGGTTTGGTTGCAACCAGCAACCCCACCCAAGGGGGGCCCTTCCATTGGTGGGTCACTACCTTGCCCTCAATTTAGACCATTTATTATGGAGCATGTAGCCCAAGAATTGAATGGTTTAGATACTAATTTCACCGGTGGTCAGCAGACAGGACTGTCAAGTTCAATCAATCAAACCCCAAGTTCAGGTTCCCAACTGTCTACTGTAAATGGAAATAGGGTTAACCTCCCTGGTTCTGCTGCTATGTCCAGGACAGGCAACAACCAAGTAGCTGGTTTAAACCGTGTCGGAAATGCTTCTCTGGTATCCTCAAATTTGGCTGTTGTGAGCTCAGCAGGGCCGGCTTTACGTAGATCCCCAGGTCCTGGTGTCCCTGCTCATGTGAGAGGAGAACTGAATACAGCCATTATTGGTCTTGGAGATGACGGAGGGTATGGAGGTGGTTGGGTTCCTCTTGTTGCTCTTAAAAAGGTGCTAAGAGGTATTCTCAAGTACCTTGGAGTTCTATGGCTATTTGCCCAGCTACCAGGGCTCTTGAAAGAGATTTTGGGGTCAATTCTGAAAGACAATGAAGGCTCACTTCTAAATTTAGACCAGGAGCAGCCCGCCTTGAGATTCTTTGTTGGTGGCTATGTATTTGCTGTAAGTGTTCACAGAGTTCAACTTCTTCTTCAAGTACTGAGCGTGAAACGATTCCATCAGCAGCAGCAACAAGGGCAACAGCCAAATACAAACACTGCCAATGAGGAATTATCTACAGCCGAAATAGGAGAAATATGTGACTACTTCAGTCGGCGTGTTGCTTCAGAGCCTTATGATGCATCTCGTGTCGCGTCATTCATTACTCTCCTTACCTTACCCATATCAGTTCTAAGAGAATTCTTGAAATTAATAGCATGGAAAAAAGGACTAGCCCAGGCACAAGGGGGAGATGTATCACCTGCTCAAAAACCCCGTATTGAATTATGCCTTGAAAACCATGCTGGCTCGAGTATGGATGGGAGCTCAGATAATTCATCTGTTGCCAAAAGTAATATTTATTATAATCGGCCTCATAACTCAGTTGATTTTGCATTGACACTTGTTCTTGATCCTGCTCACATACCTCCTATAAACGCCGCTGGTGGAGCTGCATGGTTGCCATACTGCGTCTCAGTGAGGTTGAGGTATTCATTTGGCGAAAATCCTAGCGTGTCCTTTCTTGGTATGGAAGGAAGCCATGGAGGTCGAGCTTGCTGGTTGCGCGTTGATGACTGGGAGAAGTGTAAAAACAAGGTTGCTCGAACTGTGGAACATCAGGGTAGTTCAGGAGTAGACGGTAGTCAAGGAAGGTTAAGAATCGTAGCTGACTATGTGCAAAGGACACTGCATATGTGCTTCAAGGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008284 GO:0009266 GO:0009409 GO:0009506 GO:0009605 GO:0009607 GO:0009627 GO:0009628 GO:0009631 GO:0009814 GO:0016591 GO:0016592 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032991 GO:0042127 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045087 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055029 GO:0055044 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0090575 GO:0098542 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med14

Protein Analysis

1803

Amino Acids

196.08

Weight (kDa)

8.31

Isoelectric Point (pI)

44.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med14	PF08638	9 - 199	1.2e-50	Mediator complex subunit MED14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009239)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04740
fragaria_vesca	FvH4_6g02880 FvH4_6g02880 FvH4_6g02880 FvH4_6g02880
malus_domestica	MD04G1224900.v1.1 MD12G1241600.v1.1
prunus_persica	Prupe.6G343600_v2.0.a1
pyrus_communis	pycom04g19810 pycom12g22110
rosa_chinensis	RchiOBHm_Chr3g0451091
rosa_laevigata	RLG00000025691
rosa_multiflora	Rmu_co8423931.1_g000001 Rmu_ssc0000461.1_g000005
rosa_roxburghii	Rroxscaffold_6G00428560
rosa_rugosa	Rorug02G0631200 Rorug02G0631300
rosa_samantha	Rh3AG034200 Rh3BG034500 Rh3CG033200 Rh3DG034200
rosa_wichuraiana	Rw3G002480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5064
AarI	CACCTGC	1 cut(s)	4969
AasI	GACNNNNNNGTC	2 cut(s)	3450, 4174
Acc36I	ACCTGC	2 cut(s)	3676, 4969
AccB1I	GGYRCC	3 cut(s)	109, 207, 606
AccB7I	CCANNNNNTGG	2 cut(s)	1097, 4385
AccI	GTMKAC	4 cut(s)	943, 2429, 4220, 5334
AccII	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
AcoI	YGGCCR	3 cut(s)	1552, 2145, 3886
AcyI	GRCGYC	2 cut(s)	5, 110
AflIII	ACRYGT	1 cut(s)	1345
AgeI	ACCGGT	1 cut(s)	4154
AhlI	ACTAGT	1 cut(s)	3767
AjiI	CACGTC	1 cut(s)	415
AjuI	GAANNNNNNNTTGG	8 cut(s)	630, 662, 3037, 3069, 4192, 4224, 4550, 4582
AleI	CACNNNNGTG	1 cut(s)	479
Alw21I	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4352, 5027
Alw26I	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3798, 3940, 5176
AlwNI	CAGNNNCTG	2 cut(s)	2874, 4385
Ama87I	CYCGRG	1 cut(s)	5008
ApaI	GGGCCC	3 cut(s)	2475, 3639, 4061
AseI	ATTAAT	1 cut(s)	4910
AsiGI	ACCGGT	1 cut(s)	4154
Asp700I	GAANNNNTTC	2 cut(s)	4315, 4712
AspLEI	GCGC	8 cut(s)	56, 79, 112, 717, 1353, 1355, 4005, 5265
AsuC2I	CCSGG	2 cut(s)	2407, 3690
AsuHPI	GGTGA	9 cut(s)	58, 82, 598, 662, 1225, 1366, 3014, 4144, 4950
AsuII	TTCGAA	2 cut(s)	2600, 3142
AvaI	CYCGRG	1 cut(s)	5008
AxyI	CCTNAGG	1 cut(s)	3384
BaeGI	GKGCMC	5 cut(s)	609, 797, 2475, 3639, 4061
BaeI	ACNNNNGTAYC	6 cut(s)	537, 570, 2322, 2355, 2532, 2565
BalI	TGGCCA	1 cut(s)	2147
BamHI	GGATCC	1 cut(s)	2162
BanI	GGYRCC	3 cut(s)	109, 207, 606
BarI	GAAGNNNNNNTAC	6 cut(s)	804, 836, 2209, 2241, 3103, 3135
BauI	CACGAG	2 cut(s)	3672, 4845
BbsI	GAAGAC	5 cut(s)	417, 1058, 1403, 1893, 2727
Bbv12I	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4352, 5027
BciVI	GTATCC	4 cut(s)	618, 2560, 3957, 4336
BclI	TGATCA	2 cut(s)	2278, 2865
BcnI	CCSGG	2 cut(s)	2407, 3690
BcoDI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3798, 3940, 5176
BcuI	ACTAGT	1 cut(s)	3767
BfmI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3630, 3831, 4776
BfoI	RGCGCY	1 cut(s)	113
BfuAI	ACCTGC	2 cut(s)	3676, 4969
BfuI	GTATCC	4 cut(s)	618, 2560, 3957, 4336
BlpI	GCTNAGC	1 cut(s)	4351
BmcAI	AGTACT	2 cut(s)	155, 4698
BmeT110I	CYCGRG	1 cut(s)	5008
BmgBI	CACGTC	1 cut(s)	415
BmrI	ACTGGG	2 cut(s)	1872, 5284
BmuI	ACTGGG	2 cut(s)	1872, 5284
BoxI	GACNNNNGTC	1 cut(s)	5340
BpiI	GAAGAC	5 cut(s)	417, 1058, 1403, 1893, 2727
BplI	GAGNNNNNCTC	4 cut(s)	3696, 3728, 4491, 4523
BpmI	CTGGAG	2 cut(s)	1338, 3720
Bpu1102I	GCTNAGC	1 cut(s)	4351
Bpu14I	TTCGAA	2 cut(s)	2600, 3142
BpuEI	CTTGAG	5 cut(s)	134, 267, 1589, 4493, 4651
BpuMI	CCSGG	2 cut(s)	2407, 3690
BsaAI	YACGTR	3 cut(s)	1346, 3950, 4370
BsaBI	GATNNNNATC	3 cut(s)	1784, 2346, 3968
BsaHI	GRCGYC	2 cut(s)	5, 110
BsaI	GGTCTC	2 cut(s)	2307, 2455
BsaWI	WCCGGW	2 cut(s)	1045, 4154
Bse118I	RCCGGY	3 cut(s)	454, 4154, 4360
Bse21I	CCTNAGG	1 cut(s)	3384
Bse3DI	GCAATG	2 cut(s)	1939, 3121
Bse8I	GATNNNNATC	3 cut(s)	1784, 2346, 3968
BseJI	GATNNNNATC	3 cut(s)	1784, 2346, 3968
BseMI	GCAATG	2 cut(s)	1939, 3121
BsePI	GCGCGC	1 cut(s)	1351
BseRI	GAGGAG	2 cut(s)	3717, 4422
BseSI	GKGCMC	5 cut(s)	609, 797, 2475, 3639, 4061
BseYI	CCCAGC	4 cut(s)	168, 3734, 3911, 4539
BsgI	GTGCAG	3 cut(s)	888, 1260, 3654
Bsh1236I	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
BshNI	GGYRCC	3 cut(s)	109, 207, 606
BshTI	ACCGGT	1 cut(s)	4154
BsiHKAI	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4352, 5027
BsiHKCI	CYCGRG	1 cut(s)	5008
BsiSI	CCGG	9 cut(s)	113, 184, 455, 1046, 2407, 2520, 3689, 4155, 4361
BslFI	GGGAC	7 cut(s)	203, 904, 2061, 2421, 2543, 3680, 4376
BsmAI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3798, 3940, 5176
BsmBI	CGTCTC	1 cut(s)	5176
BsmFI	GGGAC	7 cut(s)	203, 904, 2061, 2421, 2543, 3680, 4376
BsmI	GAATGC	1 cut(s)	3618
Bso31I	GGTCTC	2 cut(s)	2307, 2455
BsoBI	CYCGRG	1 cut(s)	5008
Bsp119I	TTCGAA	2 cut(s)	2600, 3142
Bsp120I	GGGCCC	3 cut(s)	2471, 3635, 4057
Bsp1720I	GCTNAGC	1 cut(s)	4351
Bsp19I	CCATGG	1 cut(s)	5239
BspFNI	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
BspHI	TCATGA	2 cut(s)	265, 744
BspMAI	CTGCAG	5 cut(s)	324, 1056, 1495, 1597, 3634
BspMI	ACCTGC	2 cut(s)	3676, 4969
BspQI	GCTCTTC	2 cut(s)	825, 2859
BspT104I	TTCGAA	2 cut(s)	2600, 3142
BspT107I	GGYRCC	3 cut(s)	109, 207, 606
BspTNI	GGTCTC	2 cut(s)	2307, 2455
BsrDI	GCAATG	2 cut(s)	1939, 3121
BsrFI	RCCGGY	3 cut(s)	454, 4154, 4360
BssAI	RCCGGY	3 cut(s)	454, 4154, 4360
BssHII	GCGCGC	1 cut(s)	1351
BssNI	GRCGYC	2 cut(s)	5, 110
BssSI	CACGAG	2 cut(s)	3672, 4845
BssT1I	CCWWGG	5 cut(s)	3125, 3286, 4050, 4516, 5239
Bst2BI	CACGAG	2 cut(s)	3672, 4845
Bst6I	CTCTTC	4 cut(s)	825, 1260, 2859, 3721
BstACI	GRCGYC	2 cut(s)	5, 110
BstAPI	GCANNNNNTGC	1 cut(s)	178
BstBAI	YACGTR	3 cut(s)	1346, 3950, 4370
BstBI	TTCGAA	2 cut(s)	2600, 3142
BstDSI	CCRYGG	1 cut(s)	5239
BstENI	CCTNNNNNAGG	2 cut(s)	1953, 3831
BstFNI	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
BstH2I	RGCGCY	1 cut(s)	113
BstHHI	GCGC	8 cut(s)	56, 79, 112, 717, 1353, 1355, 4005, 5265
BstMAI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3798, 3940, 5176
BstNSI	RCATGY	6 cut(s)	181, 2897, 3536, 3647, 4003, 4118
BstPAI	GACNNNNGTC	1 cut(s)	5340
BstSFI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3630, 3831, 4776
BstSLI	GKGCMC	5 cut(s)	609, 797, 2475, 3639, 4061
BstSNI	TACGTA	1 cut(s)	4370
BstUI	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
BstV2I	GAAGAC	5 cut(s)	417, 1058, 1403, 1893, 2727
BstX2I	RGATCY	7 cut(s)	1048, 1087, 1735, 2057, 2162, 2797, 4373
BstXI	CCANNNNNNTGG	3 cut(s)	2159, 3571, 4292
BstYI	RGATCY	7 cut(s)	1048, 1087, 1735, 2057, 2162, 2797, 4373
Bsu36I	CCTNAGG	1 cut(s)	3384
BsuI	GTATCC	4 cut(s)	618, 2560, 3957, 4336
BtgI	CCRYGG	1 cut(s)	5239
BtrI	CACGTC	1 cut(s)	415
BtsI	GCAGTG	4 cut(s)	558, 3627, 4755, 5384
BveI	ACCTGC	2 cut(s)	3676, 4969
CaiI	CAGNNNCTG	2 cut(s)	2874, 4385
CciI	TCATGA	2 cut(s)	265, 744
CfoI	GCGC	8 cut(s)	56, 79, 112, 717, 1353, 1355, 4005, 5265
Cfr10I	RCCGGY	3 cut(s)	454, 4154, 4360
CseI	GACGC	2 cut(s)	4842, 5158
CspAI	ACCGGT	1 cut(s)	4154
DinI	GGCGCC	1 cut(s)	111
DraI	TTTAAA	7 cut(s)	1473, 1702, 1714, 1761, 2695, 3358, 4299
DrdI	GACNNNNNNGTC	2 cut(s)	3450, 4174
DseDI	GACNNNNNNGTC	2 cut(s)	3450, 4174
EaeI	YGGCCR	3 cut(s)	1552, 2145, 3886
Eam1104I	CTCTTC	4 cut(s)	825, 1260, 2859, 3721
EarI	CTCTTC	4 cut(s)	825, 1260, 2859, 3721
EciI	GGCGGA	1 cut(s)	28
Ecl136II	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4350, 5025
Eco105I	TACGTA	1 cut(s)	4370
Eco130I	CCWWGG	5 cut(s)	3125, 3286, 4050, 4516, 5239
Eco31I	GGTCTC	2 cut(s)	2307, 2455
Eco32I	GATATC	4 cut(s)	1927, 2109, 2805, 2929
Eco53kI	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4350, 5025
Eco81I	CCTNAGG	1 cut(s)	3384
Eco88I	CYCGRG	1 cut(s)	5008
EcoICRI	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4350, 5025
EcoNI	CCTNNNNNAGG	2 cut(s)	1953, 3831
EcoRI	GAATTC	2 cut(s)	572, 4898
EcoRV	GATATC	4 cut(s)	1927, 2109, 2805, 2929
EcoT14I	CCWWGG	5 cut(s)	3125, 3286, 4050, 4516, 5239
EcoT22I	ATGCAT	5 cut(s)	312, 1821, 3010, 3534, 4843
EgeI	GGCGCC	1 cut(s)	111
EheI	GGCGCC	1 cut(s)	111
ErhI	CCWWGG	5 cut(s)	3125, 3286, 4050, 4516, 5239
Esp3I	CGTCTC	1 cut(s)	5176
FalI	AAGNNNNNCTT	4 cut(s)	492, 524, 4584, 4616
FaqI	GGGAC	7 cut(s)	203, 904, 2061, 2421, 2543, 3680, 4376
FauI	CCCGC	3 cut(s)	1652, 3864, 4633
FauNDI	CATATG	3 cut(s)	2533, 2975, 5391
FbaI	TGATCA	2 cut(s)	2278, 2865
FblI	GTMKAC	4 cut(s)	943, 2429, 4220, 5334
GlaI	GCGC	8 cut(s)	55, 78, 111, 716, 1352, 1354, 4004, 5264
GsaI	CCCAGC	4 cut(s)	172, 3738, 3915, 4543
GsuI	CTGGAG	2 cut(s)	1338, 3720
HaeII	RGCGCY	1 cut(s)	113
HapII	CCGG	9 cut(s)	113, 184, 455, 1046, 2407, 2520, 3689, 4155, 4361
HgaI	GACGC	2 cut(s)	4842, 5158
HhaI	GCGC	8 cut(s)	56, 79, 112, 717, 1353, 1355, 4005, 5265
Hin1I	GRCGYC	2 cut(s)	5, 110
Hin6I	GCGC	8 cut(s)	54, 77, 110, 715, 1351, 1353, 4003, 5263
HinP1I	GCGC	8 cut(s)	54, 77, 110, 715, 1351, 1353, 4003, 5263
HincII	GTYRAC	3 cut(s)	1285, 3997, 4243
HindII	GTYRAC	3 cut(s)	1285, 3997, 4243
HindIII	AAGCTT	3 cut(s)	327, 671, 1840
HpaI	GTTAAC	1 cut(s)	4243
HpaII	CCGG	9 cut(s)	113, 184, 455, 1046, 2407, 2520, 3689, 4155, 4361
HphI	GGTGA	9 cut(s)	58, 82, 598, 662, 1225, 1366, 3014, 4144, 4950
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4IV	ACGT	4 cut(s)	414, 1345, 3949, 4369
HpySE526I	ACGT	4 cut(s)	414, 1345, 3949, 4369
Hsp92I	GRCGYC	2 cut(s)	5, 110
HspAI	GCGC	8 cut(s)	54, 77, 110, 715, 1351, 1353, 4003, 5263
KasI	GGCGCC	1 cut(s)	109
KflI	GGGWCCC	1 cut(s)	3694
KroI	GCCGGC	2 cut(s)	454, 4360
KroNI	GCCGGC	2 cut(s)	456, 4362
Ksp22I	TGATCA	2 cut(s)	2278, 2865
KspAI	GTTAAC	1 cut(s)	4243
LguI	GCTCTTC	2 cut(s)	825, 2859
MaeII	ACGT	4 cut(s)	414, 1345, 3949, 4369
MaeIII	GTNAC	7 cut(s)	586, 799, 1172, 2716, 3535, 4074, 4799
MfeI	CAATTG	1 cut(s)	360
MflI	RGATCY	7 cut(s)	1048, 1087, 1735, 2057, 2162, 2797, 4373
MlsI	TGGCCA	1 cut(s)	2147
MluNI	TGGCCA	1 cut(s)	2147
Mly113I	GGCGCC	1 cut(s)	110
MlyI	GAGTC	7 cut(s)	583, 889, 3161, 3378, 3460, 3515, 3618
MmeI	TCCRAC	2 cut(s)	3421, 4288
Mox20I	TGGCCA	1 cut(s)	2147
Mph1103I	ATGCAT	5 cut(s)	312, 1821, 3010, 3534, 4843
MroNI	GCCGGC	2 cut(s)	454, 4360
MroXI	GAANNNNTTC	2 cut(s)	4315, 4712
MscI	TGGCCA	1 cut(s)	2147
MslI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
Msp20I	TGGCCA	1 cut(s)	2147
MspA1I	CMGCKG	4 cut(s)	1496, 2353, 3656, 5141
MspI	CCGG	9 cut(s)	113, 184, 455, 1046, 2407, 2520, 3689, 4155, 4361
MssI	GTTTAAAC	1 cut(s)	4299
MunI	CAATTG	1 cut(s)	360
Mva1269I	GAATGC	1 cut(s)	3618
MvnI	CGCG	7 cut(s)	54, 77, 685, 1353, 3992, 4853, 5265
NaeI	GCCGGC	2 cut(s)	456, 4362
NarI	GGCGCC	1 cut(s)	110
NciI	CCSGG	2 cut(s)	2407, 3690
NcoI	CCATGG	1 cut(s)	5239
NdeI	CATATG	3 cut(s)	2533, 2975, 5391
NgoMIV	GCCGGC	2 cut(s)	454, 4360
NmeAIII	GCCGAG	2 cut(s)	85, 3914
NmuCI	GTSAC	4 cut(s)	586, 799, 4074, 4799
NsiI	ATGCAT	5 cut(s)	312, 1821, 3010, 3534, 4843
NspI	RCATGY	6 cut(s)	181, 2897, 3536, 3647, 4003, 4118
NspV	TTCGAA	2 cut(s)	2600, 3142
OliI	CACNNNNGTG	1 cut(s)	479
PaeI	GCATGC	3 cut(s)	181, 2897, 3647
PaeR7I	CTCGAG	1 cut(s)	5008
PagI	TCATGA	2 cut(s)	265, 744
PaqCI	CACCTGC	1 cut(s)	4969
PasI	CCCWGGG	1 cut(s)	1378
PauI	GCGCGC	1 cut(s)	1351
PciSI	GCTCTTC	2 cut(s)	825, 2859
PcsI	WCGNNNNNNNCGW	1 cut(s)	3670
PctI	GAATGC	1 cut(s)	3618
PdiI	GCCGGC	2 cut(s)	456, 4362
PdmI	GAANNNNTTC	2 cut(s)	4315, 4712
PflMI	CCANNNNNTGG	2 cut(s)	1097, 4385
PfoI	TCCNGGA	2 cut(s)	3570, 4268
PinAI	ACCGGT	1 cut(s)	4154
PleI	GAGTC	7 cut(s)	583, 888, 3161, 3377, 3459, 3514, 3618
PluTI	GGCGCC	1 cut(s)	113
PmeI	GTTTAAAC	1 cut(s)	4299
PpsI	GAGTC	7 cut(s)	583, 888, 3161, 3377, 3459, 3514, 3618
Ppu21I	YACGTR	3 cut(s)	1346, 3950, 4370
PpuMI	RGGWCCY	4 cut(s)	217, 2557, 3694, 4382
PshAI	GACNNNNGTC	1 cut(s)	5340
PshBI	ATTAAT	1 cut(s)	4910
PsiI	TTATAA	1 cut(s)	5064
Psp124BI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4352, 5027
Psp5II	RGGWCCY	4 cut(s)	217, 2557, 3694, 4382
PspFI	CCCAGC	4 cut(s)	168, 3734, 3911, 4539
PspOMI	GGGCCC	3 cut(s)	2471, 3635, 4057
PspPPI	RGGWCCY	4 cut(s)	217, 2557, 3694, 4382
PspXI	VCTCGAGB	1 cut(s)	5008
PstI	CTGCAG	5 cut(s)	324, 1056, 1495, 1597, 3634
PstNI	CAGNNNCTG	2 cut(s)	2874, 4385
PsuI	RGATCY	7 cut(s)	1048, 1087, 1735, 2057, 2162, 2797, 4373
PteI	GCGCGC	1 cut(s)	1351
PvuII	CAGCTG	2 cut(s)	1496, 2353
RseI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
SacI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4352, 5027
SapI	GCTCTTC	2 cut(s)	825, 2859
ScaI	AGTACT	2 cut(s)	155, 4698
SchI	GAGTC	7 cut(s)	583, 889, 3161, 3378, 3460, 3515, 3618
SfcI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3630, 3831, 4776
SfoI	GGCGCC	1 cut(s)	111
Sfr274I	CTCGAG	1 cut(s)	5008
SfuI	TTCGAA	2 cut(s)	2600, 3142
SgrAI	CRCCGGYG	1 cut(s)	4154
SlaI	CTCGAG	1 cut(s)	5008
SmiI	ATTTAAAT	1 cut(s)	1714
SmiMI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
SmlI	CTYRAG	6 cut(s)	149, 282, 1568, 4508, 4630, 5008
SmoI	CTYRAG	6 cut(s)	149, 282, 1568, 4508, 4630, 5008
SnaBI	TACGTA	1 cut(s)	4370
SpeI	ACTAGT	1 cut(s)	3767
SphI	GCATGC	3 cut(s)	181, 2897, 3647
SspDI	GGCGCC	1 cut(s)	109
SspI	AATATT	2 cut(s)	1429, 5056
SstI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4352, 5027
StyI	CCWWGG	5 cut(s)	3125, 3286, 4050, 4516, 5239
SwaI	ATTTAAAT	1 cut(s)	1714
TaiI	ACGT	4 cut(s)	417, 1348, 3952, 4372
TaqII	GACCGA	1 cut(s)	2415
TatI	WGTACW	8 cut(s)	153, 811, 2023, 2480, 2960, 3341, 3524, 4696
TauI	GCSGC	7 cut(s)	54, 59, 688, 691, 2921, 3659, 5141
TseFI	GTSAC	4 cut(s)	586, 799, 4074, 4799
Tsp45I	GTSAC	4 cut(s)	586, 799, 4074, 4799
TspGWI	ACGGA	4 cut(s)	729, 1011, 3982, 4461
Van91I	CCANNNNNTGG	2 cut(s)	1097, 4385
VspI	ATTAAT	1 cut(s)	4910
XagI	CCTNNNNNAGG	2 cut(s)	1953, 3831
XbaI	TCTAGA	1 cut(s)	1742
XceI	RCATGY	6 cut(s)	181, 2897, 3536, 3647, 4003, 4118
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4130
XhoI	CTCGAG	1 cut(s)	5008
XmiI	GTMKAC	4 cut(s)	943, 2429, 4220, 5334
XmnI	GAANNNNTTC	2 cut(s)	4315, 4712
ZrmI	AGTACT	2 cut(s)	155, 4698
Zsp2I	ATGCAT	5 cut(s)	312, 1821, 3010, 3534, 4843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.