Rroxscaffold_6G00428560

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009239
Physical Location & Seq
Forward (+)
48984297 .. 48992806
8510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428560.1

Sequence Viewer

Length: 5331 bp
ATGGCGTCGGAGCTAGGGCAACAGACGGTGGACCTCTCCACGGTGGTGAACCGCGCCGCGGAGGAGTCGTTCCTCTCTTTAAAGGAGCTCATGGAGAAATCGAAGGCGGCGGCGCCGGAGCTCTCCGATACCGATAAGAAAATAGGGCTGCTCAAGTACTTGGTCAAGACCCAGCAACGCATGCTCCGGCTCAATGTTCTCGCCAAGTGGTGCCAGCAGGTCCCATTGATACAATATTGCCAGCAACTTACTTCTACGTTGTCAAGTCATGATACCTGTTTTACTCAAGCAGCAGATTCATTGTTTTTCATGCATGAAGGTCTACAACAAGCTTGTGCTCCTGTTTATGATGTTCCATCTGCAGTTGAAATCCTCCTATCAGGATCATATCAGCGGTTACCAAAATGTATAGAAGATGTGGGTCTTCAGAGTTCGCTAAGTGAGGATGAGCAGAAGCCGGCTTTGAAAAAACTGGACATGCTTGTGCGGAGACAATTACTAGAAGTGTCAATCCCAAAAGAAATAAGTGAGGTTAAAGTCTCTGATGGTACAGCACTGCTTCGTGTAAATGGGGAATTCAAGGTTTTAATTACTCTTGGCTACCGAGGGCACCTATCAATGTGGAGGATACTGCACTTGGAGCTGCTTGTTGGCGAGAGAAGTGGACTTATAAAGCTAGAAGTATCTCAGCGGTATACTCTTGGAGATGATTTAGAGCGCAGAATGGCTGCTGCAGAAAATCCATTCAAGACATTGTACTCTTTTCTACATGAGATGTGTGTTAAACTTGTTATAGACACTGTCTTAAGGCAAGTACAGGCTCTTCGCCTGGGGAGATGGAAGGATGCAATCCGATTTGAGGTCCTATCTGATGGGAGCACCAGTCATGCAGGGACTTCTGGCTCTGCGCAATTAAATCAAGATGGAGAAACTGAAACATCTGGTTTACGAACGCCAGGGTTAAAAATTGTATACTGGTTAGATTTCGATAAAAACAGTGGTATTTCTGATTCATCATTATGTCCGTCTATAAAAATTGATCCAGGACCAGATCTACTGATAAAATGTGTTCATAGCACATTTGTAATAGATCCATTAACTGGAAAGGAGGCAGAATTCTCTCTTGACCAAAGTTGTATTGATGTTGAGAAATTGCTTCTGAGAGCCATATGCTGTAATAGATATACTCGTCTTCTCGAAATTCAGAAAGAGCTGGAGAAAAACGTTCAAATCTGTCGAGGTTCAGGGGATGTTGCTTTTCAGTCCTGTGTGGAAGAAGCGGACAGTGACCATAAAAAGAAAGATTTTAAGTCAGATGTCAGAGAATATGAAGGGCACGAAGTATTACGTGTGCGTGCCTATGGGAACGGCCGTTTTCGTCTTCAGTCCTCCCGGAATATTCTTGCTTCTTCTGCATTTTTGTCTGAATGTGAAGATGCTTTAAATCAGGGAACTATGACTGCAGCCGAAGTTTTCATTAGCTTGAGAAGCAAAAGTATTTTGCACTTATTTGCATCTATTGGAAGGTTTTTAGGCCTTGAGGTATATGAAAATGGTTTACCTGCAGTTAAATTACCAAAGAACATTTTGAATGGCTCAACTATGTTGCTAATGGGATTTCCAGATTGTGGGAGCTCTTATTTTTTGCTGATGCAGCTTGATAAGGATTTCAAACCCCTTTTTAAATTGCTTGAGACTCAGCCCGATTCTTCTGGAAAAGCTGATTCTCTCAATGACCAAAATCATGTGATACGGATCAAGAAAATTGATGTTAACCAGATGCAGATGCATGAAGATGATATGAATTTAAGCTTGCTTGACTGGGGAAAACTACCATCCATATTGCCTAGCGCTGGTAGATCTAATCAGTCATCTGAAAATGGTCTTCTTACAGATATCAGCCTTGAGGGTTCTATGCCAATTGCAGGGTGTCCACCATCGAGTTTTTCTTCTGTGGTTGATGAAGTGTTTGAACTGGAGAAAGGGTTATCTGCACCTTCCTTCTCTCTTCAAAATGGAACTTCATCATTCAACACATCTTCTGCCTCTCATTTTGGCTCTGCTCCAATGAATCTTCATTCCATAAAAGCTGGAAGTCCAGCTTCCAAATGGGAGGGAGGTATGCAGATGTCGCAGTTAAACAGTGCTGCGAATGTTTCTGGTATGGCAGCCCATTATAATGGATCCTTGTATTCATCAAGTAACTTGAAGGGCACCATACAGTCTTCTTCTCTCAGTTCTCTGTCTTCTGCTACTGGAAGGAGTGTCTCTGTGAAGAAAATGTCTGTTTCAAAGTCTGATCAGGATTTGGCTTCGCTTAGGTCTCCCCAGTTAGTAGATTATGGTTCTACTTCAATGGATGAAGATCACCTGAGATTTATGAGTGATACTTCAAAGGGTGCAACATATGGATTCAGATCTTCTCACTTATTATCTCCACCTGGTCCCAGTGGCCCCCGAATATCAGGGCCAGGCATGAGACCTGTAACTGGATCTTTCAGAGTCACTGGTTCAAACTCATGTGTAACAATTCCTGCAACCCATGCACCTGATTCTGGAGTCTGCGATGGCCCTAATCATGATGATTCAAATCATGATAGAAAACTTCGAAAGCGTACACTTTCAGAAATGTTGAATCTGATCCCATCACTGCAAGGTGTGGAAGCCAATTTAGGATCTTGTAAAAGGAGGAAAGTTTCTGAAGTCGATCAGGCTCAGCACTCTTCATCGCAGGTGTTAATGTCAACGGATATGACTTCAAAAACTGGATTATACAGTTATGGGGATCTTATATCTGAAGCTAATAAGGGATATGCACCTTCTAGCATTTATGTTTCAGCTCTTCTTCATGTGGTCAGGCACTGTTCACTTGGTATTAAGCATGCCAGACTAACTAGCCAGATGGGGGCTCTGGACATCCCTTATGTTGAAGAAGTGGGGTTAAGAAGTACATCTTCAAACATATGGTTCCGACTTCCATTTGCCAGGGGAGATTCATGGCAACACCTGTGCTTGCGACTTGGCAGAGCTGGAAGCATATATTGGGATGTAAAAATAAATGACCAGCATTTCCGCGATTTGTGGGAGCTTCAGAAAGGAAGCAATACTACACCTTGGGGTTCTGGTGTTCGTATTGCCAATACTTCTGACATTGACTCTCATATTCGTTACGATCCAGAAGGTGTTGTTCTCAGTTATCAGTCTGTGGAGGCTGATAGTATAAAGAAGTTAGTGGCAGATATCCAAAGGCTTTCTAATGCAAGAATGTTTTCCCTGGGGATGCGGAAACTGCTTGGAGTAAGAGCAGATGAGAAACCAGAAGAAAGTGTAAACTCCGATTCTAAAGCACCAGGAGGCAAAGGTTCTTTTGAGGGAGCTGATAGGTTATCTGAACAAATGAGAAGGGCATTTAGAATTGAGGCAGTAGGACTCATGAGTTTGTGGTTCAGTTTTGGATCTGGTGTCCTGGCTCGCTTTGTTGTTGAGTGGGAATCAGGTAAAGAGGGTTGTACTATGCACGTATCTCCTGACCAACTTTGGCCTCATACCAAGTTTCTGGAAGATTTCATAAACGGAGCTGAAGTTGCATCACTTTTGGACTGCATTCGCCTCACTGCGGGTCCCTTACATGCTCTTGCAGCTGCAACTCGACCGGCGCGAGCTGGACCTATTCCAGGGGTTATTGGGATGACAATTCTCTCTTCTGTCCCAAAACAGGCTGGGTACATACCATCCCAGGGCCTTATGCAAACCAGTTCAACCACAAACGTTAGTCAGTCTCCTGTCCCTGTTGGGAACTCTGTTTCATCTGCTGCTACAGGTCCTCTAACAAATCACGGCCTTCACGGGGCTGCAATGTTAGCTGCTGCTGCTGGCCGAGGTGGACCTGGCATTGTACCTAGCTCACTGTTGCCCATTGATGTTTCTGTTGTGTTGCGTGGTCCATACTGGATACGAATTATATATCGGAAACACTTTGCAGTTGACATGCGATGTTTTGCAGGAGATCAGGTCTGGTTACAACCAGCAACGCCACCAAAGGGGGCCCTTCAGTTGGAGACGGGATTGGCAAATTTACACACTCAAAACCTCAGTGCAGGTCCCCAGCTGTCTGCTGTCAATGGAAACAGGGTTAACCTCCCTAGTTCTGCTGCCTTGTCTAGGACAGGCAATCAAGTAGCTAGTTTGAACCGTGCTGGAAATGCTTCTCCAGTATCCTCGAATTTGGCTGTCATGAGCTCAGGGATGCCTTTACGTAGATCACCAGGTGCAGGTGTCCCTGCTCATGTGAGAGGAGAACTGAATACAGCCATTATTGGTCTTGGAGATGATGGGGGGTATGGAGGTGGTTGGGTCCCCCTTGTTGCTCTTAAAAAGGTGCTGAGAGGTATTCTCAAGTACCTTGGAGTGCTGTGGCTATTTGCCCAGCTGCCCGATCTTTTGAAAGAGATTCTAGGGTCCATTTTGAAAGACAATGAAGGTGCACTATTAAATTTGGACCAGGAGCAGCCTGCTTTGCGTTTCTTTGTGGGTGGCTATGTATTTGCAGTAAGTGTTCATAGAGTTCAACTTCTTCTTCAAGTCCTGAGTGTGAAAAGATTCCATCATCAGCAGCAGCAACAGCAGCAAAATCCAACTACTGCTCAAGAGGAATTGACTCCAACTGAAATAGGAGAAATTTGTGACTACTTTAGTCGACGTGTTGCATCAGAGCCTTACGATGCTTCTCGTGTTGCATCATTCATCACTCTTCTGACCTTACCCATATCAGTTCTGAGAGAATTCTTGAAATTAATAGCATGGAAAAAAGGACTAGCTCAGCAGGTGCAGGGTGGTGATGTAGCTCCTGCTCAGAAACCCCGTATTGAATTATGTCTTGAATATCATGCTGGGTCGAATATAGATGAGAACTCAGATAATTCGTCTGTCGCCAAAAGTAATATCCATTATGATCGGCCTCACAACTCGGTTGATTTTGCACTGACTCTTGTTCTTGATTCTGCTCACATACCCCACATAAATGCCGCAGGCGGTGCAGCATGGCTGCCTTACTGTGTATCAGTGAAGTTGAGGTATTCATTTGGTGAAAATCCTAATGTGTCCTTTCTTGGCATGGAAGGAAGCCATGGAGGTCGAGCTTGCTGGTTGCGTGTTGATGACTGGGAGAAGTGTAAACAGAAGGTTGCTCGGACTGTGGAGAGTTGTTCAGGAGGAGACAATAGTCTCGGAAGGTTGAGACTTGTTGCTGACTATGTGCAAAGAACGCTACATGTGTGGCTTCAAGGTTTGAGGGATGGCGGTGTGGTTAGTTCAACCCCTGGGGGCACATGA

Protein Analysis

1776

Amino Acids

193.26

Weight (kDa)

7.26

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med14 PF08638 9 - 200 3.7e-50 Mediator complex subunit MED14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 671, 2178
AarI CACCTGC 3 cut(s) 2722, 4262, 4813
Acc16I TGCGCA 1 cut(s) 909
Acc36I ACCTGC 6 cut(s) 208, 1570, 2722, 4088, 4262, 4813
AccB1I GGYRCC 4 cut(s) 112, 210, 609, 2213
AccB7I CCANNNNNTGG 2 cut(s) 1100, 1628
AccI GTMKAC 4 cut(s) 322, 695, 972, 4696
AccII CGCG 4 cut(s) 54, 59, 3075, 3658
AclI AACGTT 2 cut(s) 1224, 3768
AcoI YGGCCR 2 cut(s) 1369, 3874
AcsI RAATTY 9 cut(s) 575, 1115, 1200, 1804, 4072, 4222, 4492, 4677, 4781
AcuI CTGAAG 7 cut(s) 410, 1367, 2721, 2817, 3074, 3600, 4034
AcyI GRCGYC 2 cut(s) 5, 113
AdeI CACNNNGTG 1 cut(s) 4268
AfeI AGCGCT 1 cut(s) 1852
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 3 cut(s) 1348, 4699, 5269
AjiI CACGTC 1 cut(s) 4700
AjuI GAANNNNNNNTTGG 2 cut(s) 3025, 3057
AleI CACNNNNGTG 1 cut(s) 44
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
Alw21I GWGCWC 7 cut(s) 90, 123, 340, 881, 1637, 4241, 4486
Alw44I GTGCAC 1 cut(s) 4482
AlwNI CAGNNNCTG 1 cut(s) 2862
Aor51HI AGCGCT 1 cut(s) 1852
ApaI GGGCCC 1 cut(s) 4048
ApaLI GTGCAC 1 cut(s) 4482
ApoI RAATTY 9 cut(s) 575, 1115, 1200, 1804, 4072, 4222, 4492, 4677, 4781
AseI ATTAAT 1 cut(s) 4793
Asp700I GAANNNNTTC 3 cut(s) 3268, 4204, 4598
AspLEI GCGC 6 cut(s) 56, 115, 720, 910, 1853, 3658
AsuC2I CCSGG 1 cut(s) 1393
AsuHPI GGTGA 5 cut(s) 58, 2360, 4254, 4847, 5096
AsuII TTCGAA 1 cut(s) 2608
BaeGI GKGCMC 6 cut(s) 612, 1338, 2216, 4048, 4486, 5327
BamHI GGATCC 1 cut(s) 2183
BanI GGYRCC 4 cut(s) 112, 210, 609, 2213
BanII GRGCYC 6 cut(s) 90, 123, 1637, 2911, 4048, 4241
BarI GAAGNNNNNNTAC 4 cut(s) 807, 839, 3091, 3123
BauI CACGAG 1 cut(s) 4728
BbsI GAAGAC 6 cut(s) 416, 1184, 1373, 1877, 2217, 2238
Bbv12I GWGCWC 7 cut(s) 90, 123, 340, 881, 1637, 4241, 4486
BceAI ACGGC 3 cut(s) 1356, 1384, 3853
BciVI GTATCC 3 cut(s) 621, 3945, 4225
BclI TGATCA 1 cut(s) 2299
BcnI CCSGG 1 cut(s) 1393
BfmI CTRYAG 5 cut(s) 360, 732, 1461, 1563, 3816
BfoI RGCGCY 2 cut(s) 116, 1854
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 6 cut(s) 208, 1570, 2722, 4088, 4262, 4813
BfuI GTATCC 3 cut(s) 621, 3945, 4225
BglII AGATCT 3 cut(s) 1051, 1859, 2417
BlpI GCTNAGC 2 cut(s) 2715, 4818
BmcAI AGTACT 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 4700
BmrI ACTGGG 4 cut(s) 1831, 2323, 2442, 5170
BmuI ACTGGG 4 cut(s) 1831, 2323, 2442, 5170
BoxI GACNNNNGTC 1 cut(s) 5220
BpiI GAAGAC 6 cut(s) 416, 1184, 1373, 1877, 2217, 2238
BplI GAGNNNNNCTC 2 cut(s) 4377, 4409
BpmI CTGGAG 4 cut(s) 1235, 1997, 2577, 4194
Bpu10I CCTNAGC 2 cut(s) 2318, 4240
Bpu1102I GCTNAGC 2 cut(s) 2715, 4818
Bpu14I TTCGAA 1 cut(s) 2608
BpuEI CTTGAG 8 cut(s) 137, 270, 1504, 1559, 1712, 1925, 4379, 4629
BpuMI CCSGG 1 cut(s) 1393
BsaAI YACGTR 3 cut(s) 1349, 3520, 4256
BsaBI GATNNNNATC 4 cut(s) 1754, 2364, 2416, 2589
BsaHI GRCGYC 2 cut(s) 5, 113
BsaI GGTCTC 2 cut(s) 2328, 2473
Bse118I RCCGGY 2 cut(s) 457, 3652
Bse3DI GCAATG 1 cut(s) 3861
Bse8I GATNNNNATC 4 cut(s) 1754, 2364, 2416, 2589
BseJI GATNNNNATC 4 cut(s) 1754, 2364, 2416, 2589
BseMI GCAATG 1 cut(s) 3861
BseRI GAGGAG 3 cut(s) 77, 4308, 5226
BseSI GKGCMC 6 cut(s) 612, 1338, 2216, 4048, 4486, 5327
BseX3I CGGCCG 1 cut(s) 1369
BseYI CCCAGC 5 cut(s) 171, 3719, 4104, 4425, 4889
BsgI GTGCAG 6 cut(s) 617, 1977, 4116, 4290, 4847, 5055
Bsh1236I CGCG 4 cut(s) 54, 59, 3075, 3658
Bsh1285I CGRYCG 2 cut(s) 1372, 3653
BshNI GGYRCC 4 cut(s) 112, 210, 609, 2213
BsiEI CGRYCG 2 cut(s) 1372, 3653
BsiHKAI GWGCWC 7 cut(s) 90, 123, 340, 881, 1637, 4241, 4486
BsiSI CCGG 5 cut(s) 116, 187, 458, 1393, 3653
BslFI GGGAC 9 cut(s) 206, 907, 2430, 3606, 3692, 3770, 4086, 4262, 4340
BsmBI CGTCTC 1 cut(s) 4052
BsmFI GGGAC 9 cut(s) 206, 907, 2430, 3606, 3692, 3770, 4086, 4262, 4340
BsmI GAATGC 1 cut(s) 3603
Bso31I GGTCTC 2 cut(s) 2328, 2473
Bsp119I TTCGAA 1 cut(s) 2608
Bsp120I GGGCCC 1 cut(s) 4044
Bsp1720I GCTNAGC 2 cut(s) 2715, 4818
Bsp19I CCATGG 1 cut(s) 5125
BspFNI CGCG 4 cut(s) 54, 59, 3075, 3658
BspHI TCATGA 5 cut(s) 268, 2578, 2593, 3432, 4233
BspMAI CTGCAG 4 cut(s) 364, 736, 1465, 1567
BspMI ACCTGC 6 cut(s) 208, 1570, 2722, 4088, 4262, 4813
BspQI GCTCTTC 2 cut(s) 828, 2847
BspT104I TTCGAA 1 cut(s) 2608
BspT107I GGYRCC 4 cut(s) 112, 210, 609, 2213
BspTI CTTAAG 1 cut(s) 805
BspTNI GGTCTC 2 cut(s) 2328, 2473
BsrDI GCAATG 1 cut(s) 3861
BsrFI RCCGGY 2 cut(s) 457, 3652
BssAI RCCGGY 2 cut(s) 457, 3652
BssNAI GTATAC 2 cut(s) 696, 973
BssNI GRCGYC 2 cut(s) 5, 113
BssSI CACGAG 1 cut(s) 4728
BssT1I CCWWGG 3 cut(s) 3113, 4402, 5125
Bst1107I GTATAC 2 cut(s) 696, 973
Bst2BI CACGAG 1 cut(s) 4728
Bst6I CTCTTC 6 cut(s) 828, 2013, 2728, 2847, 3706, 4755
BstACI GRCGYC 2 cut(s) 5, 113
BstAFI CTTAAG 1 cut(s) 805
BstAPI GCANNNNNTGC 3 cut(s) 181, 2543, 5033
BstBAI YACGTR 3 cut(s) 1349, 3520, 4256
BstBI TTCGAA 1 cut(s) 2608
BstDSI CCRYGG 3 cut(s) 39, 57, 5125
BstEII GGTNACC 1 cut(s) 396
BstENI CCTNNNNNAGG 2 cut(s) 3672, 4159
BstFNI CGCG 4 cut(s) 54, 59, 3075, 3658
BstH2I RGCGCY 2 cut(s) 116, 1854
BstHHI GCGC 6 cut(s) 56, 115, 720, 910, 1853, 3658
BstMCI CGRYCG 2 cut(s) 1372, 3653
BstNSI RCATGY 6 cut(s) 184, 481, 2885, 3632, 3991, 5273
BstPAI GACNNNNGTC 1 cut(s) 5220
BstPI GGTNACC 1 cut(s) 396
BstSFI CTRYAG 5 cut(s) 360, 732, 1461, 1563, 3816
BstSLI GKGCMC 6 cut(s) 612, 1338, 2216, 4048, 4486, 5327
BstSNI TACGTA 1 cut(s) 4256
BstUI CGCG 4 cut(s) 54, 59, 3075, 3658
BstV2I GAAGAC 6 cut(s) 416, 1184, 1373, 1877, 2217, 2238
BstX2I RGATCY 9 cut(s) 1051, 1090, 1859, 2183, 2417, 2492, 2675, 2785, 3455
BstXI CCANNNNNNTGG 2 cut(s) 2180, 3556
BstYI RGATCY 9 cut(s) 1051, 1090, 1859, 2183, 2417, 2492, 2675, 2785, 3455
BstZ17I GTATAC 2 cut(s) 696, 973
BstZI CGGCCG 1 cut(s) 1369
BsuI GTATCC 3 cut(s) 621, 3945, 4225
BtgI CCRYGG 3 cut(s) 39, 57, 5125
BtgZI GCGATG 3 cut(s) 2580, 2712, 4006
BtrI CACGTC 1 cut(s) 4700
BtsI GCAGTG 3 cut(s) 554, 2648, 3612
BveI ACCTGC 6 cut(s) 208, 1570, 2722, 4088, 4262, 4813
CaiI CAGNNNCTG 1 cut(s) 2862
CciI TCATGA 5 cut(s) 268, 2578, 2593, 3432, 4233
CfoI GCGC 6 cut(s) 56, 115, 720, 910, 1853, 3658
Cfr10I RCCGGY 2 cut(s) 457, 3652
Cfr42I CCGCGG 1 cut(s) 60
CsiI ACCWGGT 2 cut(s) 2440, 4264
DinI GGCGCC 1 cut(s) 114
DraI TTTAAA 3 cut(s) 81, 1443, 1684
DraIII CACNNNGTG 1 cut(s) 4268
EaeI YGGCCR 2 cut(s) 1369, 3874
EagI CGGCCG 1 cut(s) 1369
Eam1104I CTCTTC 6 cut(s) 828, 2013, 2728, 2847, 3706, 4755
EarI CTCTTC 6 cut(s) 828, 2013, 2728, 2847, 3706, 4755
Ecl136II GAGCTC 4 cut(s) 88, 121, 1635, 4239
EclXI CGGCCG 1 cut(s) 1369
Eco105I TACGTA 1 cut(s) 4256
Eco130I CCWWGG 3 cut(s) 3113, 4402, 5125
Eco147I AGGCCT 1 cut(s) 1536
Eco24I GRGCYC 6 cut(s) 90, 123, 1637, 2911, 4048, 4241
Eco31I GGTCTC 2 cut(s) 2328, 2473
Eco32I GATATC 2 cut(s) 1897, 3241
Eco47III AGCGCT 1 cut(s) 1852
Eco52I CGGCCG 1 cut(s) 1369
Eco53kI GAGCTC 4 cut(s) 88, 121, 1635, 4239
Eco57I CTGAAG 7 cut(s) 410, 1367, 2721, 2817, 3074, 3600, 4034
Eco91I GGTNACC 1 cut(s) 396
EcoICRI GAGCTC 4 cut(s) 88, 121, 1635, 4239
EcoNI CCTNNNNNAGG 2 cut(s) 3672, 4159
EcoO109I RGGNCCY 9 cut(s) 220, 862, 3620, 3739, 3821, 4044, 4045, 4100, 4354
EcoO65I GGTNACC 1 cut(s) 396
EcoRI GAATTC 3 cut(s) 575, 1115, 4781
EcoRV GATATC 2 cut(s) 1897, 3241
EcoT14I CCWWGG 3 cut(s) 3113, 4402, 5125
EcoT22I ATGCAT 2 cut(s) 315, 1791
EcoT38I GRGCYC 6 cut(s) 90, 123, 1637, 2911, 4048, 4241
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
ErhI CCWWGG 3 cut(s) 3113, 4402, 5125
Esp3I CGTCTC 1 cut(s) 4052
FalI AAGNNNNNCTT 4 cut(s) 2086, 2118, 2938, 2970
FaqI GGGAC 9 cut(s) 206, 907, 2430, 3606, 3692, 3770, 4086, 4262, 4340
FauI CCCGC 1 cut(s) 3610
FauNDI CATATG 3 cut(s) 1169, 2407, 2963
FbaI TGATCA 1 cut(s) 2299
FblI GTMKAC 4 cut(s) 322, 695, 972, 4696
FriOI GRGCYC 6 cut(s) 90, 123, 1637, 2911, 4048, 4241
FspI TGCGCA 1 cut(s) 909
GlaI GCGC 6 cut(s) 55, 114, 719, 909, 1852, 3657
GsaI CCCAGC 5 cut(s) 175, 3723, 4108, 4429, 4893
GsuI CTGGAG 4 cut(s) 1235, 1997, 2577, 4194
HaeII RGCGCY 2 cut(s) 116, 1854
HapII CCGG 5 cut(s) 116, 187, 458, 1393, 3653
HhaI GCGC 6 cut(s) 56, 115, 720, 910, 1853, 3658
Hin1I GRCGYC 2 cut(s) 5, 113
Hin6I GCGC 6 cut(s) 54, 113, 718, 908, 1851, 3656
HinP1I GCGC 6 cut(s) 54, 113, 718, 908, 1851, 3656
HincII GTYRAC 5 cut(s) 1774, 2745, 3985, 4135, 4697
HindII GTYRAC 5 cut(s) 1774, 2745, 3985, 4135, 4697
HindIII AAGCTT 2 cut(s) 330, 1810
HpaI GTTAAC 2 cut(s) 1774, 4135
HpaII CCGG 5 cut(s) 116, 187, 458, 1393, 3653
HphI GGTGA 5 cut(s) 58, 2360, 4254, 4847, 5096
Hpy99I CGWCG 2 cut(s) 10, 4701
HpyCH4IV ACGT 7 cut(s) 257, 1224, 1348, 3519, 3768, 4255, 4699
HpySE526I ACGT 7 cut(s) 257, 1224, 1348, 3519, 3768, 4255, 4699
Hsp92I GRCGYC 2 cut(s) 5, 113
HspAI GCGC 6 cut(s) 54, 113, 718, 908, 1851, 3656
KasI GGCGCC 1 cut(s) 112
KflI GGGWCCC 2 cut(s) 3620, 4354
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 2299
KspAI GTTAAC 2 cut(s) 1774, 4135
KspI CCGCGG 1 cut(s) 60
LguI GCTCTTC 2 cut(s) 828, 2847
MabI ACCWGGT 2 cut(s) 2440, 4264
MaeII ACGT 7 cut(s) 257, 1224, 1348, 3519, 3768, 4255, 4699
MaeIII GTNAC 9 cut(s) 396, 1286, 2201, 2485, 2503, 2524, 3167, 4017, 4682
MfeI CAATTG 1 cut(s) 1920
MflI RGATCY 9 cut(s) 1051, 1090, 1859, 2183, 2417, 2492, 2675, 2785, 3455
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 8 cut(s) 74, 1690, 2511, 2568, 3149, 3423, 4651, 4978
MmeI TCCRAC 4 cut(s) 2995, 4035, 4658, 4685
Mph1103I ATGCAT 2 cut(s) 315, 1791
MroNI GCCGGC 1 cut(s) 457
MroXI GAANNNNTTC 3 cut(s) 3268, 4204, 4598
MslI CAYNNNNRTG 6 cut(s) 44, 482, 1018, 1794, 2178, 5019
MspA1I CMGCKG 6 cut(s) 59, 394, 691, 3641, 4108, 4429
MspCI CTTAAG 1 cut(s) 805
MspI CCGG 5 cut(s) 116, 187, 458, 1393, 3653
MunI CAATTG 1 cut(s) 1920
Mva1269I GAATGC 1 cut(s) 3603
MvnI CGCG 4 cut(s) 54, 59, 3075, 3658
NaeI GCCGGC 1 cut(s) 459
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 1 cut(s) 1393
NcoI CCATGG 1 cut(s) 5125
NdeI CATATG 3 cut(s) 1169, 2407, 2963
NgoMIV GCCGGC 1 cut(s) 457
NmeAIII GCCGAG 1 cut(s) 3902
NmuCI GTSAC 3 cut(s) 1286, 2503, 4682
NsbI TGCGCA 1 cut(s) 909
NsiI ATGCAT 2 cut(s) 315, 1791
NspI RCATGY 6 cut(s) 184, 481, 2885, 3632, 3991, 5273
NspV TTCGAA 1 cut(s) 2608
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 2 cut(s) 184, 2885
PagI TCATGA 5 cut(s) 268, 2578, 2593, 3432, 4233
PaqCI CACCTGC 3 cut(s) 2722, 4262, 4813
PasI CCCWGGG 3 cut(s) 3274, 3736, 5318
PceI AGGCCT 1 cut(s) 1536
PciI ACATGT 1 cut(s) 5269
PciSI GCTCTTC 2 cut(s) 828, 2847
PcsI WCGNNNNNNNCGW 1 cut(s) 3655
PctI GAATGC 1 cut(s) 3603
PdiI GCCGGC 1 cut(s) 459
PdmI GAANNNNTTC 3 cut(s) 3268, 4204, 4598
PflFI GACNNNGTC 1 cut(s) 800
PflMI CCANNNNNTGG 2 cut(s) 1100, 1628
PfoI TCCNGGA 2 cut(s) 1042, 1391
PleI GAGTC 8 cut(s) 73, 1690, 2510, 2567, 3149, 3423, 4651, 4978
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 8 cut(s) 73, 1690, 2510, 2567, 3149, 3423, 4651, 4978
Ppu21I YACGTR 3 cut(s) 1349, 3520, 4256
PpuMI RGGWCCY 6 cut(s) 220, 862, 3620, 3821, 4100, 4354
PscI ACATGT 1 cut(s) 5269
PshAI GACNNNNGTC 1 cut(s) 5220
PshBI ATTAAT 1 cut(s) 4793
PsiI TTATAA 2 cut(s) 671, 2178
Psp124BI GAGCTC 4 cut(s) 90, 123, 1637, 4241
Psp1406I AACGTT 2 cut(s) 1224, 3768
Psp5II RGGWCCY 6 cut(s) 220, 862, 3620, 3821, 4100, 4354
PspEI GGTNACC 1 cut(s) 396
PspFI CCCAGC 5 cut(s) 171, 3719, 4104, 4425, 4889
PspOMI GGGCCC 1 cut(s) 4044
PspPPI RGGWCCY 6 cut(s) 220, 862, 3620, 3821, 4100, 4354
PstI CTGCAG 4 cut(s) 364, 736, 1465, 1567
PstNI CAGNNNCTG 1 cut(s) 2862
PsuI RGATCY 9 cut(s) 1051, 1090, 1859, 2183, 2417, 2492, 2675, 2785, 3455
PsyI GACNNNGTC 1 cut(s) 800
PvuII CAGCTG 3 cut(s) 3641, 4108, 4429
RseI CAYNNNNRTG 6 cut(s) 44, 482, 1018, 1794, 2178, 5019
SacI GAGCTC 4 cut(s) 90, 123, 1637, 4241
SacII CCGCGG 1 cut(s) 60
SalI GTCGAC 1 cut(s) 4695
SapI GCTCTTC 2 cut(s) 828, 2847
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 8 cut(s) 74, 1690, 2511, 2568, 3149, 3423, 4651, 4978
SexAI ACCWGGT 2 cut(s) 2440, 4264
SfcI CTRYAG 5 cut(s) 360, 732, 1461, 1563, 3816
SfoI GGCGCC 1 cut(s) 114
Sfr303I CCGCGG 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 2608
SgrBI CCGCGG 1 cut(s) 60
SmiMI CAYNNNNRTG 6 cut(s) 44, 482, 1018, 1794, 2178, 5019
SmlI CTYRAG 9 cut(s) 152, 285, 805, 1483, 1538, 1691, 1904, 4394, 4644
SmoI CTYRAG 9 cut(s) 152, 285, 805, 1483, 1538, 1691, 1904, 4394, 4644
SnaBI TACGTA 1 cut(s) 4256
SphI GCATGC 2 cut(s) 184, 2885
SseBI AGGCCT 1 cut(s) 1536
SspDI GGCGCC 1 cut(s) 112
SspI AATATT 2 cut(s) 236, 1399
SstI GAGCTC 4 cut(s) 90, 123, 1637, 4241
StuI AGGCCT 1 cut(s) 1536
StyI CCWWGG 3 cut(s) 3113, 4402, 5125
TaiI ACGT 7 cut(s) 260, 1227, 1351, 3522, 3771, 4258, 4702
TatI WGTACW 5 cut(s) 156, 756, 814, 2948, 3509
TauI GCSGC 4 cut(s) 59, 110, 113, 5027
TseFI GTSAC 3 cut(s) 1286, 2503, 4682
Tsp45I GTSAC 3 cut(s) 1286, 2503, 4682
TspGWI ACGGA 4 cut(s) 1014, 1768, 2762, 3588
Tth111I GACNNNGTC 1 cut(s) 800
Van91I CCANNNNNTGG 2 cut(s) 1100, 1628
Vha464I CTTAAG 1 cut(s) 805
VneI GTGCAC 1 cut(s) 4482
VspI ATTAAT 1 cut(s) 4793
XagI CCTNNNNNAGG 2 cut(s) 3672, 4159
XapI RAATTY 9 cut(s) 575, 1115, 1200, 1804, 4072, 4222, 4492, 4677, 4781
XceI RCATGY 6 cut(s) 184, 481, 2885, 3632, 3991, 5273
XcmI CCANNNNNNNNNTGG 1 cut(s) 2106
XmiI GTMKAC 4 cut(s) 322, 695, 972, 4696
XmnI GAANNNNTTC 3 cut(s) 3268, 4204, 4598
ZrmI AGTACT 1 cut(s) 158
Zsp2I ATGCAT 2 cut(s) 315, 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.