Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0009239

Physical Location & Seq

Genomic Coordinates Forward (+)

2279152 .. 2288282

Gene Structure 9131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG034200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5151 bp

ATGCATGAAGGTCTACAACAAGCTTGTGCTCCTGTTTATGATGTTCCATCTGCAGTTGAAATCCTCCTATCAGGATCATATCAGCGGTTACCAAAATGTATAGAAGATGTGGGTCTTCAGAGTTCACTAAGTGAGGATGAGCAGAAGCCGGCTTTGAAAAAACTGGACATGCTTGTGCGGAGACAATTACTAGAAGTGTCAATCCCAAAAGAAATAAGCGAGGTTAAAGTCTCTGATGGTACGGCACTGCTTCGTGTAAATGGGGAATTCAAGGTTTTAATTACTCTTGGCTACCGAGGGCACCTATCAATGTGGAGGATACTGCACTTGGAGCTGCTTGTTGGCGAGAGAAGTGGACTTATAAAGCTAGAAGTATCTCAGCGGTATACTCTTGGGGATGATTTAGAGCGCAGAATGGCTGCTGCAGAAAATCCATTCAAGACATTGTACTCTTTTCTACATGAGATGTGTGTTAAACTTGTTATAGACACTGTCTTAAGGCAAGTACAGGCTCTTCGCCAGGGGAGATGGAAGGATGCAATCCGATTTGAGGTCCTATCTGATGGGAGCACCAGTCATGCAGGGACTTCTGGCTCTGCGCAATTAAATCAAGATGGAGAAACTGAAACATCTGGTTTACGAACGCCAGGGTTAAAAATTGTATACTGGTTAGATTTCGATAAAAACAGTGGTATTTCTGATTCATCATTATGTCCGTCTATAAAAATTGATCCAGGACCAGATCTACTGATAAAATGTGTTCATAGCACATTTGTAATAGATCCATTAACTGGAAAGGAGGCAGAATTCTCTCTTGACCAAAGTTGTATTGATGTTGAAAAATTGCTTCTGAGAGCCATATGCTGTAATAGATATACTCGTCTTCTTGAAATTCAGAAAGAGCTGGAGAAAAACGTTCAAATCTGTCGAGGTTCAGGGGATGTTGCTTTTCAGTCCTGTGTGGAAGAAGTGGACAGTGACCATAAAAAGAAAGATTTTAAGTCAGATGTCAGAGAATATGAAGGGCACGAAGTATTATGTGTGCGTGCCTATGGGTCATCATTTTTTACTCTGGGAATAAATATAAGGAACGGCCGCTTTCGTCTTCAGTCCTCCCGGAATATTCTTGCTTCTTCGGCATTTTTGTCTGAATGTGAAGATGCTTTAAATCAGGGAACTATGACTGCAGCCGAAGTTTTCATTAGCTTGAGAAGCAAAAGTATTTTGCACTTATTTGCATCTATTGGAAGGTTTTTAGGCCTTGAGGTATATGAAAATGGTTTACCTGCAGTTAAATTACCAAAGAACATTTTGAATGGCTCAACTATGTTGCTGATGGGATTTCCAGATTGTGGGAGCTCTTATTTTTTGCTGATGCAGCTTGATAAGGATTTCAAACCCCTTTTTAAATTGCTTGAGACTCAGCCGGATTCTTCTGGAAAAGCTGATTCTCTCAATGACCAAAATCATGTGATACGGATCAAGAAAATTGATGTTAACCAGATGCAGATGCATGAAGATGATATGAATTTAAGCTTGCTTGACTGGGGAAAACTACAATCCATATTGTCTAGCGCTGGTAGATCTAATCAGTCATCTGAAAATGGTCTTCTTATGGATATCAGCCTCGAGGGTTCTATGCCAATTGCTGGGTGTCCACCATCGAGTTTTTCTTCTGTGGTTGATGAAGTGTTTGAACTGGAGAAAGGGTTATCTGCACCTTCCTTCTCTCTTCAAAATGGAACTTCATCATTCAACGCATCTTCTGCCTCTCATTTTGGCTCTGCTCCAATGAATCTTCATTCCATAAAAGCTGGAAGTCCAGCTTCCAAATGGGAGGGAGGTATGCAGATGTCGCAGTTAAACAGTGCTGCAAATGGTTCTGGTATGGCAGCCCATTATAATGGATCCTTGCATTCATCAAGTAACTTGAAGGGCACCATACAGTCTTCTTCTCTCAGTTCTCTGTCTTCTGCTACTGGAAGGAGTGTCTCTGTGAAGAAAATGTCTGTTTCAAAGTCTGATCAGGATTTGGCTTCGCTTAGGTCTCCCCAGTTAGTAGATTATGGTTCTACTTCAATGGATGAAGATCACGTGAGATTTATGAGTGATACTTCAAAGGGTGCAACATATGGATTTAGATCTTCTCGCCTATTATCTCCACCTGGCCCCAGTGGCCCCCGAATATCCGGGCCAGGCATGAGACCTGTAACTGGATCTTTCAGAGTCGCTGGTTCAAACTCATGTGTAACAACTCCTGCAACCCATGCACCTGATTCTGGAGTCTGCGATGGCCCTAATCATGATGATTCAAATCATGATAGAAAACTTCGAAAGCGTACACTTTCAGAAATGTTGAATCTGATCCCATCACTGCAAGGTGTGGAAGCCAATTTAGGATCTTGTAAAAGGAGGAAAGTTTCTGAAGTCGATCAGGCTCAGCACTCTTCATCGCAGGTGTTAATGTCAACGGATATGACTTCAAAAACTGGATTATACAGTTATGGGGATCTTATATCTGAAGCTAATAAGGGATATGCACCTTCTAGCATTTATGTTTCAGCTCTTCTTCATGTGGTCAGGCACTGTTCACTTGGTATTAAGCATGCCAGACTAACTAGCCAGATGGGGGCTCTGGACATTCCTTATGTTGAAGAAGTGGGGTTAAGAAGTACATCTTCAAACATATGGTTCCGACTTCCATTTGCCAGGGGTGATTCATGGCAACACCTGTGCTTGCGACTTGGCAGAGCTGGAAGCATATATTGGGATGTAAAAATAAATGACCAGCATTTCCGCGATTTGTGGGAGCTTCAAAAAGGAAGCAATACTACACCTTGGGGTTCTGGTGTTCGTATTGCCAATACTTCTGACATTGACTCTCATATTCGTTACGATCCAGAAGGTGTTGTTCTCAGTTATCAGTCTGTGGAGGCTGATAGTATAAAGAAGTTAGTGGCAGATATCCAAAGGCTTTCTAATGCAAGAATGTTTTCCCTGGGGATGCGGAAACTGCTTGGAGTAAGACCAGATGAGAAACCAGAAGAAAGTGTAAACTCCGATTCTAAAGCACCAGGAGGCAAAGGTTCTTTTGAGGGAGCTGATAGGTTATCTGAACAAATGAGAAGGGCATTTAGAATTGAGGCAGTAGGACTCATGAGTTTGTGGTTCAGTTTTGGATCTGGTGTCCTGGCTCGCTTTGTTGTTGAGTGGGAATCAGGTAAAGAGGGTTGTACTATGCACGTATCTCCTGACCAACTTTGGCCTCATACCAAGTTTCTGGAAGATTTCATAAACGGAGCTGAAGTTGCATCACTTTTGGACTGCATTCGCCTCACTGCTGGTCCCTTACATGCTCTTGCAGCTGCAACTCGACCGGCGCGAGCTGGTCCTATTCCAGGGGTTAGTGGGATGACAATTCTCTCTTCTGTCCCAAAACAGGCCGGGTACATACCATCCCAGGGCCTTATGCAAACCAGTTCAACCACAAACGTTAGTCAGTCTCCTGTCCCTGTTGGGAACTCTGTTTCATCTGCTGCTACAGGTCCTCTTACAAATCACGGCCTTCACGGGGCTGCAATGTTAGCTGCTGCTGCTGGCCGAGGTGGACCTGGCATTGTACCTAGCTCACTGTTGCCCATTGATGTTTCTGTTGTGCTGCGTGGTCCATACTGGATACGAATTATATATCGGAAGCACTTTGCAGTTGACATGCGATGTTTTGCAGGAGATCAGGTCTGGTTACAACCAGCAACGCCACCAAAGGGGGGCCCTTCAGTTGGAGGTTCTTTGCCTTGCCCACAATTTCGGCCATTTATTATGGAGCATGTGGCCCAAGAATTAAATGGTTTAGATACTAATTTCAATGGTGTTCAACAAACGGGATTGGCAAATTTACACACTCAAAACCTCGGTGCAGGTCCCCAGCTGTCTGCTGTCAATGGAAATAGGGTTAACCTCCCTAGTTCTGCTGCATTGTCTAGGACAGGCAATCAAGTAGCTAGTTTGAACCGTGCTGGAAATGCTTCTCCAGTGTCCTCGAATTTGGCTGTCATGAGCTCAGGGATGCCTTTACGTAGATCACCAGGTGCAGGTGTCCCTGCTCATGTGAGAGGAGAACTGAATACAGCCATTATTGGTCTTGGAGATGATGGGGGGTATGGAGGTGGTTGGGTCCCCCTTGTTGCTCTTAAAAAGGTGCTGAGAGGTATTCTCAAGTACCTTGGAGTGCTGTGGCTATTTGCCCAGCTGCCCGATCTTTTGAAAGAGATTCTAGGGTCCATTTTGAAAGACAATGAAGGTGCACTATTAAATTTGGACCAGGAGCAGCCTGCTTTGCGTTTCTTTGTGGGTGGCTATGTATTTGCAGTAAGTGTTCATAGAGTTCAACTTCTTCTTCAAGTCCTGAGTGTGAAAAGATTCCATCATCAGCAGCAGCAACAGCAGCAAAATCCAACCACTGCTCAAGAGGAATTGACTCCAACTGAAATAGGAGAAATTTGCGACTACTTTAGTCGACGTGTTGCATCAGAGCCTTACGATGCTTCTCGTGTTGCATCATTCATCACTCTTCTGACCTTACCCATATCAGTTCTGAGAGAATTCTTGAAATTAATAGCATGGAAAAAAGGACTAGCCCAGCAGGTGCAGGGTGGTGATGTAGCTCCTGCTCAGAAGCCCCGTATTGAATTATGTCTTGAATATCATGCTGGGTCGAATATAGATGAGAACTCAGATAATTCATCTGTTGCCAAAAGTAATATCCATTATGATCGGCCTCACAACTCGGTTGATTTTGCACTGACTCTTGTTCTTGATTCTGCTCACATACCCCACATAAATGCCGCAGGCGGTGCAGCATGGCTGCCTTACTGTGTATCAGTGAAGTTGAGGTATTCATTTGGCGAAAATCCTAATGTATCCTTTCTTGGCATGGAAGGAAGCCATGGAGGTCGAGCTTGCTGGTTGCGTGTTGATGACTGGGAGAAGTGTAAACAAAAGGTTGCTCGGACTGTGGAGAGTTGTTCAGGAGGAGACAATAGTCTCGGAAGGTTGAGACTTGTTGCTGACTATGTGCAAAGAACGCTACATGTGTGGCTTCAAGGTTTGAGGGATGGCGGTGTGGTTAGTTCAACCCCCGGGGGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008284 GO:0009266 GO:0009409 GO:0009506 GO:0009605 GO:0009607 GO:0009627 GO:0009628 GO:0009631 GO:0009814 GO:0016591 GO:0016592 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032991 GO:0042127 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045087 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055029 GO:0055044 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0090575 GO:0098542 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med14

Protein Analysis

1716

Amino Acids

186.23

Weight (kDa)

7.27

Isoelectric Point (pI)

44.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med14	PF08638	1 - 97	3.2e-21	Mediator complex subunit MED14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009239)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04740
fragaria_vesca	FvH4_6g02880 FvH4_6g02880 FvH4_6g02880 FvH4_6g02880
malus_domestica	MD04G1224900.v1.1 MD12G1241600.v1.1
prunus_persica	Prupe.6G343600_v2.0.a1
pyrus_communis	pycom04g19810 pycom12g22110
rosa_chinensis	RchiOBHm_Chr3g0451091
rosa_laevigata	RLG00000025691
rosa_multiflora	Rmu_co8423931.1_g000001 Rmu_ssc0000461.1_g000005
rosa_roxburghii	Rroxscaffold_6G00428560
rosa_rugosa	Rorug02G0631200 Rorug02G0631300
rosa_samantha	Rh3AG034200 Rh3BG034500 Rh3CG033200 Rh3DG034200
rosa_wichuraiana	Rw3G002480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	362, 1902
AarI	CACCTGC	3 cut(s)	2446, 4082, 4633
AbsI	CCTCGAGG	1 cut(s)	1628
Acc16I	TGCGCA	1 cut(s)	600
Acc36I	ACCTGC	5 cut(s)	1294, 2446, 3908, 4082, 4633
AccB1I	GGYRCC	2 cut(s)	300, 1937
AccB7I	CCANNNNNTGG	3 cut(s)	791, 1352, 1649
AccI	GTMKAC	4 cut(s)	13, 386, 663, 4516
AccII	CGCG	2 cut(s)	2799, 3382
AciI	CCGC	9 cut(s)	85, 178, 382, 1096, 2797, 3007, 4845, 4851, 5118
AclI	AACGTT	2 cut(s)	915, 3492
AcoI	YGGCCR	3 cut(s)	1093, 3598, 3809
AcsI	RAATTY	9 cut(s)	266, 806, 891, 1528, 3892, 4042, 4312, 4497, 4601
AcuI	CTGAAG	6 cut(s)	101, 1091, 2445, 2541, 3324, 3759
AcvI	CACGTG	1 cut(s)	2095
AdeI	CACNNNGTG	2 cut(s)	131, 4088
AfaI	GTAC	9 cut(s)	241, 449, 507, 2341, 2674, 3235, 3449, 3621, 4220
AfeI	AGCGCT	1 cut(s)	1576
AflII	CTTAAG	1 cut(s)	496
AflIII	ACRYGT	2 cut(s)	4519, 5089
AjiI	CACGTC	1 cut(s)	4520
AjuI	GAANNNNNNNTTGG	2 cut(s)	2749, 2781
Alw21I	GWGCWC	5 cut(s)	31, 572, 1361, 4061, 4306
Alw44I	GTGCAC	1 cut(s)	4302
AlwNI	CAGNNNCTG	1 cut(s)	2586
Ama87I	CYCGRG	2 cut(s)	1628, 5138
Aor51HI	AGCGCT	1 cut(s)	1576
ApaI	GGGCCC	1 cut(s)	3773
ApaLI	GTGCAC	1 cut(s)	4302
ApoI	RAATTY	9 cut(s)	266, 806, 891, 1528, 3892, 4042, 4312, 4497, 4601
AseI	ATTAAT	1 cut(s)	4613
Asp700I	GAANNNNTTC	3 cut(s)	2992, 4024, 4418
AspLEI	GCGC	4 cut(s)	411, 601, 1577, 3382
AsuC2I	CCSGG	5 cut(s)	1117, 2191, 3445, 5139, 5140
AsuHPI	GGTGA	3 cut(s)	2726, 4074, 4667
AsuII	TTCGAA	1 cut(s)	2332
AvaI	CYCGRG	2 cut(s)	1628, 5138
BaeGI	GKGCMC	6 cut(s)	303, 1029, 1940, 3773, 4306, 5147
BamHI	GGATCC	1 cut(s)	1907
BanI	GGYRCC	2 cut(s)	300, 1937
BanII	GRGCYC	4 cut(s)	1361, 2635, 3773, 4061
BarI	GAAGNNNNNNTAC	4 cut(s)	498, 530, 2815, 2847
BauI	CACGAG	1 cut(s)	4548
BbrPI	CACGTG	1 cut(s)	2095
BbsI	GAAGAC	6 cut(s)	107, 875, 1097, 1601, 1941, 1962
Bbv12I	GWGCWC	5 cut(s)	31, 572, 1361, 4061, 4306
BceAI	ACGGC	3 cut(s)	258, 1108, 3577
BciVI	GTATCC	3 cut(s)	312, 3669, 4930
BclI	TGATCA	1 cut(s)	2023
BcnI	CCSGG	5 cut(s)	1117, 2191, 3445, 5139, 5140
BfmI	CTRYAG	5 cut(s)	51, 423, 1185, 1287, 3540
BfoI	RGCGCY	1 cut(s)	1578
BfrI	CTTAAG	1 cut(s)	496
BfuAI	ACCTGC	5 cut(s)	1294, 2446, 3908, 4082, 4633
BfuI	GTATCC	3 cut(s)	312, 3669, 4930
BglI	GCCNNNNNGGC	1 cut(s)	2175
BglII	AGATCT	3 cut(s)	742, 1583, 2141
BlpI	GCTNAGC	1 cut(s)	2439
BmeT110I	CYCGRG	2 cut(s)	1628, 5138
BmgBI	CACGTC	1 cut(s)	4520
BmrI	ACTGGG	4 cut(s)	1555, 2047, 2166, 4990
BmuI	ACTGGG	4 cut(s)	1555, 2047, 2166, 4990
BoxI	GACNNNNGTC	1 cut(s)	5040
BpiI	GAAGAC	6 cut(s)	107, 875, 1097, 1601, 1941, 1962
BplI	GAGNNNNNCTC	2 cut(s)	4197, 4229
BpmI	CTGGAG	4 cut(s)	926, 1721, 2301, 4014
Bpu10I	CCTNAGC	2 cut(s)	2042, 4060
Bpu1102I	GCTNAGC	1 cut(s)	2439
Bpu14I	TTCGAA	1 cut(s)	2332
BpuEI	CTTGAG	5 cut(s)	1228, 1283, 1436, 4199, 4449
BpuMI	CCSGG	5 cut(s)	1117, 2191, 3445, 5139, 5140
BsaAI	YACGTR	3 cut(s)	2095, 3244, 4076
BsaBI	GATNNNNATC	4 cut(s)	1478, 2088, 2140, 2313
BsaI	GGTCTC	2 cut(s)	2052, 2197
BsaXI	ACNNNNNCTCC	8 cut(s)	559, 589, 1694, 1724, 3291, 3321, 3720, 3750
Bse118I	RCCGGY	2 cut(s)	148, 3376
Bse3DI	GCAATG	1 cut(s)	3585
Bse8I	GATNNNNATC	4 cut(s)	1478, 2088, 2140, 2313
BseJI	GATNNNNATC	4 cut(s)	1478, 2088, 2140, 2313
BseMI	GCAATG	1 cut(s)	3585
BseRI	GAGGAG	2 cut(s)	4128, 5046
BseSI	GKGCMC	6 cut(s)	303, 1029, 1940, 3773, 4306, 5147
BseX3I	CGGCCG	1 cut(s)	1093
BseYI	CCCAGC	5 cut(s)	1649, 3924, 4245, 4638, 4709
BsgI	GTGCAG	6 cut(s)	308, 1701, 3936, 4110, 4667, 4875
Bsh1236I	CGCG	2 cut(s)	2799, 3382
Bsh1285I	CGRYCG	2 cut(s)	1096, 3377
BshNI	GGYRCC	2 cut(s)	300, 1937
BsiEI	CGRYCG	2 cut(s)	1096, 3377
BsiHKAI	GWGCWC	5 cut(s)	31, 572, 1361, 4061, 4306
BsiHKCI	CYCGRG	2 cut(s)	1628, 5138
BsiSI	CCGG	7 cut(s)	149, 1117, 1427, 2190, 3377, 3444, 5139
BslFI	GGGAC	7 cut(s)	598, 3330, 3416, 3494, 3906, 4082, 4160
BsmFI	GGGAC	7 cut(s)	598, 3330, 3416, 3494, 3906, 4082, 4160
BsmI	GAATGC	2 cut(s)	1915, 3327
Bso31I	GGTCTC	2 cut(s)	2052, 2197
BsoBI	CYCGRG	2 cut(s)	1628, 5138
Bsp119I	TTCGAA	1 cut(s)	2332
Bsp120I	GGGCCC	1 cut(s)	3769
Bsp1720I	GCTNAGC	1 cut(s)	2439
Bsp19I	CCATGG	1 cut(s)	4945
BspACI	CCGC	9 cut(s)	85, 178, 382, 1096, 2797, 3007, 4845, 4851, 5118
BspFNI	CGCG	2 cut(s)	2799, 3382
BspHI	TCATGA	4 cut(s)	2302, 2317, 3156, 4053
BspMAI	CTGCAG	4 cut(s)	55, 427, 1189, 1291
BspMI	ACCTGC	5 cut(s)	1294, 2446, 3908, 4082, 4633
BspQI	GCTCTTC	2 cut(s)	519, 2571
BspT104I	TTCGAA	1 cut(s)	2332
BspT107I	GGYRCC	2 cut(s)	300, 1937
BspTI	CTTAAG	1 cut(s)	496
BspTNI	GGTCTC	2 cut(s)	2052, 2197
BsrDI	GCAATG	1 cut(s)	3585
BsrFI	RCCGGY	2 cut(s)	148, 3376
BssAI	RCCGGY	2 cut(s)	148, 3376
BssNAI	GTATAC	2 cut(s)	387, 664
BssSI	CACGAG	1 cut(s)	4548
BssT1I	CCWWGG	3 cut(s)	2837, 4222, 4945
Bst1107I	GTATAC	2 cut(s)	387, 664
Bst2BI	CACGAG	1 cut(s)	4548
Bst6I	CTCTTC	6 cut(s)	519, 1737, 2452, 2571, 3430, 4575
BstAFI	CTTAAG	1 cut(s)	496
BstAPI	GCANNNNNTGC	3 cut(s)	1766, 2267, 4853
BstBAI	YACGTR	3 cut(s)	2095, 3244, 4076
BstBI	TTCGAA	1 cut(s)	2332
BstDSI	CCRYGG	1 cut(s)	4945
BstEII	GGTNACC	1 cut(s)	87
BstENI	CCTNNNNNAGG	1 cut(s)	3396
BstFNI	CGCG	2 cut(s)	2799, 3382
BstH2I	RGCGCY	1 cut(s)	1578
BstHHI	GCGC	4 cut(s)	411, 601, 1577, 3382
BstMCI	CGRYCG	2 cut(s)	1096, 3377
BstNSI	RCATGY	6 cut(s)	172, 2609, 3356, 3715, 3830, 5093
BstPAI	GACNNNNGTC	1 cut(s)	5040
BstPI	GGTNACC	1 cut(s)	87
BstSFI	CTRYAG	5 cut(s)	51, 423, 1185, 1287, 3540
BstSLI	GKGCMC	6 cut(s)	303, 1029, 1940, 3773, 4306, 5147
BstSNI	TACGTA	1 cut(s)	4076
BstUI	CGCG	2 cut(s)	2799, 3382
BstV2I	GAAGAC	6 cut(s)	107, 875, 1097, 1601, 1941, 1962
BstX2I	RGATCY	9 cut(s)	742, 781, 1583, 1907, 2141, 2216, 2399, 2509, 3179
BstXI	CCANNNNNNTGG	2 cut(s)	1904, 3280
BstYI	RGATCY	9 cut(s)	742, 781, 1583, 1907, 2141, 2216, 2399, 2509, 3179
BstZ17I	GTATAC	2 cut(s)	387, 664
BstZI	CGGCCG	1 cut(s)	1093
BsuI	GTATCC	3 cut(s)	312, 3669, 4930
BtgI	CCRYGG	1 cut(s)	4945
BtgZI	GCGATG	3 cut(s)	2304, 2436, 3730
BtrI	CACGTC	1 cut(s)	4520
BtsI	GCAGTG	4 cut(s)	245, 2372, 3336, 4458
BveI	ACCTGC	5 cut(s)	1294, 2446, 3908, 4082, 4633
CaiI	CAGNNNCTG	1 cut(s)	2586
CciI	TCATGA	4 cut(s)	2302, 2317, 3156, 4053
CfoI	GCGC	4 cut(s)	411, 601, 1577, 3382
Cfr10I	RCCGGY	2 cut(s)	148, 3376
Cfr9I	CCCGGG	1 cut(s)	5138
CsiI	ACCWGGT	1 cut(s)	4084
Csp6I	GTAC	9 cut(s)	240, 448, 506, 2340, 2673, 3234, 3448, 3620, 4219
CviQI	GTAC	9 cut(s)	240, 448, 506, 2340, 2673, 3234, 3448, 3620, 4219
DraI	TTTAAA	2 cut(s)	1167, 1408
DraIII	CACNNNGTG	2 cut(s)	131, 4088
EaeI	YGGCCR	3 cut(s)	1093, 3598, 3809
EagI	CGGCCG	1 cut(s)	1093
Eam1104I	CTCTTC	6 cut(s)	519, 1737, 2452, 2571, 3430, 4575
EarI	CTCTTC	6 cut(s)	519, 1737, 2452, 2571, 3430, 4575
Ecl136II	GAGCTC	2 cut(s)	1359, 4059
EclXI	CGGCCG	1 cut(s)	1093
Eco105I	TACGTA	1 cut(s)	4076
Eco130I	CCWWGG	3 cut(s)	2837, 4222, 4945
Eco147I	AGGCCT	1 cut(s)	1260
Eco24I	GRGCYC	4 cut(s)	1361, 2635, 3773, 4061
Eco31I	GGTCTC	2 cut(s)	2052, 2197
Eco32I	GATATC	2 cut(s)	1621, 2965
Eco47III	AGCGCT	1 cut(s)	1576
Eco52I	CGGCCG	1 cut(s)	1093
Eco53kI	GAGCTC	2 cut(s)	1359, 4059
Eco57I	CTGAAG	6 cut(s)	101, 1091, 2445, 2541, 3324, 3759
Eco72I	CACGTG	1 cut(s)	2095
Eco88I	CYCGRG	2 cut(s)	1628, 5138
Eco91I	GGTNACC	1 cut(s)	87
EcoICRI	GAGCTC	2 cut(s)	1359, 4059
EcoNI	CCTNNNNNAGG	1 cut(s)	3396
EcoO109I	RGGNCCY	7 cut(s)	553, 3463, 3545, 3769, 3770, 3920, 4174
EcoO65I	GGTNACC	1 cut(s)	87
EcoRI	GAATTC	3 cut(s)	266, 806, 4601
EcoRV	GATATC	2 cut(s)	1621, 2965
EcoT14I	CCWWGG	3 cut(s)	2837, 4222, 4945
EcoT22I	ATGCAT	2 cut(s)	6, 1515
EcoT38I	GRGCYC	4 cut(s)	1361, 2635, 3773, 4061
ErhI	CCWWGG	3 cut(s)	2837, 4222, 4945
FalI	AAGNNNNNCTT	4 cut(s)	1810, 1842, 2662, 2694
FaqI	GGGAC	7 cut(s)	598, 3330, 3416, 3494, 3906, 4082, 4160
FauNDI	CATATG	3 cut(s)	860, 2131, 2687
FbaI	TGATCA	1 cut(s)	2023
FblI	GTMKAC	4 cut(s)	13, 386, 663, 4516
FriOI	GRGCYC	4 cut(s)	1361, 2635, 3773, 4061
FspI	TGCGCA	1 cut(s)	600
GlaI	GCGC	4 cut(s)	410, 600, 1576, 3381
GsaI	CCCAGC	5 cut(s)	1653, 3928, 4249, 4642, 4713
GsuI	CTGGAG	4 cut(s)	926, 1721, 2301, 4014
HaeII	RGCGCY	1 cut(s)	1578
HapII	CCGG	7 cut(s)	149, 1117, 1427, 2190, 3377, 3444, 5139
HhaI	GCGC	4 cut(s)	411, 601, 1577, 3382
Hin6I	GCGC	4 cut(s)	409, 599, 1575, 3380
HinP1I	GCGC	4 cut(s)	409, 599, 1575, 3380
HincII	GTYRAC	5 cut(s)	1498, 2469, 3709, 3955, 4517
HindII	GTYRAC	5 cut(s)	1498, 2469, 3709, 3955, 4517
HindIII	AAGCTT	2 cut(s)	21, 1534
HpaI	GTTAAC	2 cut(s)	1498, 3955
HpaII	CCGG	7 cut(s)	149, 1117, 1427, 2190, 3377, 3444, 5139
HphI	GGTGA	3 cut(s)	2726, 4074, 4667
Hpy99I	CGWCG	1 cut(s)	4521
HpyCH4IV	ACGT	6 cut(s)	915, 2094, 3243, 3492, 4075, 4519
HpySE526I	ACGT	6 cut(s)	915, 2094, 3243, 3492, 4075, 4519
HspAI	GCGC	4 cut(s)	409, 599, 1575, 3380
KflI	GGGWCCC	1 cut(s)	4174
KroI	GCCGGC	1 cut(s)	148
KroNI	GCCGGC	1 cut(s)	150
Ksp22I	TGATCA	1 cut(s)	2023
KspAI	GTTAAC	2 cut(s)	1498, 3955
LguI	GCTCTTC	2 cut(s)	519, 2571
MabI	ACCWGGT	1 cut(s)	4084
MaeII	ACGT	6 cut(s)	915, 2094, 3243, 3492, 4075, 4519
MaeIII	GTNAC	7 cut(s)	87, 977, 1925, 2209, 2248, 2891, 3741
MfeI	CAATTG	1 cut(s)	1644
MflI	RGATCY	9 cut(s)	742, 781, 1583, 1907, 2141, 2216, 2399, 2509, 3179
MlyI	GAGTC	7 cut(s)	1414, 2235, 2292, 2873, 3147, 4471, 4798
MmeI	TCCRAC	4 cut(s)	2719, 3760, 4478, 4505
Mph1103I	ATGCAT	2 cut(s)	6, 1515
MroNI	GCCGGC	1 cut(s)	148
MroXI	GAANNNNTTC	3 cut(s)	2992, 4024, 4418
MslI	CAYNNNNRTG	5 cut(s)	173, 709, 1518, 1902, 4839
MspA1I	CMGCKG	5 cut(s)	85, 382, 3365, 3928, 4249
MspCI	CTTAAG	1 cut(s)	496
MspI	CCGG	7 cut(s)	149, 1117, 1427, 2190, 3377, 3444, 5139
MunI	CAATTG	1 cut(s)	1644
Mva1269I	GAATGC	2 cut(s)	1915, 3327
MvnI	CGCG	2 cut(s)	2799, 3382
NaeI	GCCGGC	1 cut(s)	150
NciI	CCSGG	5 cut(s)	1117, 2191, 3445, 5139, 5140
NcoI	CCATGG	1 cut(s)	4945
NdeI	CATATG	3 cut(s)	860, 2131, 2687
NgoMIV	GCCGGC	1 cut(s)	148
NmeAIII	GCCGAG	1 cut(s)	3626
NmuCI	GTSAC	1 cut(s)	977
NsbI	TGCGCA	1 cut(s)	600
NsiI	ATGCAT	2 cut(s)	6, 1515
NspI	RCATGY	6 cut(s)	172, 2609, 3356, 3715, 3830, 5093
NspV	TTCGAA	1 cut(s)	2332
PaeI	GCATGC	1 cut(s)	2609
PaeR7I	CTCGAG	1 cut(s)	1628
PagI	TCATGA	4 cut(s)	2302, 2317, 3156, 4053
PaqCI	CACCTGC	3 cut(s)	2446, 4082, 4633
PasI	CCCWGGG	2 cut(s)	2998, 3460
PceI	AGGCCT	1 cut(s)	1260
PciI	ACATGT	1 cut(s)	5089
PciSI	GCTCTTC	2 cut(s)	519, 2571
PcsI	WCGNNNNNNNCGW	1 cut(s)	3379
PctI	GAATGC	2 cut(s)	1915, 3327
PdiI	GCCGGC	1 cut(s)	150
PdmI	GAANNNNTTC	3 cut(s)	2992, 4024, 4418
PflFI	GACNNNGTC	1 cut(s)	491
PflMI	CCANNNNNTGG	3 cut(s)	791, 1352, 1649
PfoI	TCCNGGA	2 cut(s)	733, 1115
PleI	GAGTC	7 cut(s)	1414, 2234, 2291, 2873, 3147, 4471, 4798
PmaCI	CACGTG	1 cut(s)	2095
PmlI	CACGTG	1 cut(s)	2095
PpsI	GAGTC	7 cut(s)	1414, 2234, 2291, 2873, 3147, 4471, 4798
Ppu21I	YACGTR	3 cut(s)	2095, 3244, 4076
PpuMI	RGGWCCY	4 cut(s)	553, 3545, 3920, 4174
PscI	ACATGT	1 cut(s)	5089
PshAI	GACNNNNGTC	1 cut(s)	5040
PshBI	ATTAAT	1 cut(s)	4613
PsiI	TTATAA	2 cut(s)	362, 1902
Psp124BI	GAGCTC	2 cut(s)	1361, 4061
Psp1406I	AACGTT	2 cut(s)	915, 3492
Psp5II	RGGWCCY	4 cut(s)	553, 3545, 3920, 4174
PspCI	CACGTG	1 cut(s)	2095
PspEI	GGTNACC	1 cut(s)	87
PspFI	CCCAGC	5 cut(s)	1649, 3924, 4245, 4638, 4709
PspOMI	GGGCCC	1 cut(s)	3769
PspPPI	RGGWCCY	4 cut(s)	553, 3545, 3920, 4174
PspXI	VCTCGAGB	1 cut(s)	1628
PstI	CTGCAG	4 cut(s)	55, 427, 1189, 1291
PstNI	CAGNNNCTG	1 cut(s)	2586
PsuI	RGATCY	9 cut(s)	742, 781, 1583, 1907, 2141, 2216, 2399, 2509, 3179
PsyI	GACNNNGTC	1 cut(s)	491
PvuII	CAGCTG	3 cut(s)	3365, 3928, 4249
RsaI	GTAC	9 cut(s)	241, 449, 507, 2341, 2674, 3235, 3449, 3621, 4220
RsaNI	GTAC	9 cut(s)	240, 448, 506, 2340, 2673, 3234, 3448, 3620, 4219
RseI	CAYNNNNRTG	5 cut(s)	173, 709, 1518, 1902, 4839
SacI	GAGCTC	2 cut(s)	1361, 4061
SalI	GTCGAC	1 cut(s)	4515
SapI	GCTCTTC	2 cut(s)	519, 2571
SchI	GAGTC	7 cut(s)	1414, 2235, 2292, 2873, 3147, 4471, 4798
SexAI	ACCWGGT	1 cut(s)	4084
SfcI	CTRYAG	5 cut(s)	51, 423, 1185, 1287, 3540
SfiI	GGCCNNNNNGGCC	1 cut(s)	2175
Sfr274I	CTCGAG	1 cut(s)	1628
SfuI	TTCGAA	1 cut(s)	2332
SlaI	CTCGAG	1 cut(s)	1628
SmaI	CCCGGG	1 cut(s)	5140
SmiMI	CAYNNNNRTG	5 cut(s)	173, 709, 1518, 1902, 4839
SmlI	CTYRAG	7 cut(s)	496, 1207, 1262, 1415, 1628, 4214, 4464
SmoI	CTYRAG	7 cut(s)	496, 1207, 1262, 1415, 1628, 4214, 4464
SnaBI	TACGTA	1 cut(s)	4076
SphI	GCATGC	1 cut(s)	2609
SseBI	AGGCCT	1 cut(s)	1260
SsiI	CCGC	9 cut(s)	85, 178, 382, 1096, 2797, 3007, 4845, 4851, 5118
SspI	AATATT	1 cut(s)	1123
SstI	GAGCTC	2 cut(s)	1361, 4061
StuI	AGGCCT	1 cut(s)	1260
StyI	CCWWGG	3 cut(s)	2837, 4222, 4945
TaiI	ACGT	6 cut(s)	918, 2097, 3246, 3495, 4078, 4522
TatI	WGTACW	4 cut(s)	447, 505, 2672, 3233
TauI	GCSGC	2 cut(s)	1098, 4847
TseFI	GTSAC	1 cut(s)	977
Tsp45I	GTSAC	1 cut(s)	977
TspGWI	ACGGA	4 cut(s)	705, 1492, 2486, 3312
TspMI	CCCGGG	1 cut(s)	5138
Tth111I	GACNNNGTC	1 cut(s)	491
Van91I	CCANNNNNTGG	3 cut(s)	791, 1352, 1649
Vha464I	CTTAAG	1 cut(s)	496
VneI	GTGCAC	1 cut(s)	4302
VspI	ATTAAT	1 cut(s)	4613
XagI	CCTNNNNNAGG	1 cut(s)	3396
XapI	RAATTY	9 cut(s)	266, 806, 891, 1528, 3892, 4042, 4312, 4497, 4601
XceI	RCATGY	6 cut(s)	172, 2609, 3356, 3715, 3830, 5093
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1830, 3842
XhoI	CTCGAG	1 cut(s)	1628
XmaI	CCCGGG	1 cut(s)	5138
XmiI	GTMKAC	4 cut(s)	13, 386, 663, 4516
XmnI	GAANNNNTTC	3 cut(s)	2992, 4024, 4418
Zsp2I	ATGCAT	2 cut(s)	6, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.