MD04G1232100.v1.1

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015642
Physical Location & Seq
Forward (+)
31135912 .. 31138020
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1232100.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGATTCAACTTTTCTCCGTTTTGCATCGCTACTACTTCTACTTTCTCTCGCATCATCTGCAAACCCTAATTCCGACTACAACAGTTTGGTATACAAGAAATGTGCAAATCAAACATTTACAACTCTCTCAACTAAATCGCAAGTCCAGCAACAAACCCTTTCATCTTTTTTTCAACAACTAGCATCACACTCCTCCCAATCAAAGTTCTATAAGCACGCCGAAGCCAGCGGAGACAACAACGAGGCGGGTATTTCCGGCCTCTTCCAGTGCCGGCAGGATATCAGCAACGAGGAGTGTCACACCTGCGTGAAAACTCTTCGTAACTTATCAAACGCCTTGTGTAAAGAGTCGGTTTCGGCTCGTGTTCAGCTGCATGGGTGCTACGTGCACTACGAGCTCGATGGAGTCGACTCAATCCCTTCTAGCCATAGATTAATGCAGAAGACTTGTGGGAAGGATTTGGCAAATGGGGTGGCGGCAGGGTTTGAGAAAATGAGAGCTGCGGCGTTTGCTGCCTTGGAGAGCGGCGTTGCGGACGGTGGTGGGTTTTGCCAAACGAGTTATGAGGCGGTGCAAGTTATGGCTCAGTGTGTCGGGGGCTTGGGGGGTTGCGCGTGTGGAGAGTGTGTGAGTAGGGCGGTACAAACTGCTGAAGAGGAATGTGGTGGTGCTGTTTCTGGGCAGATTTATTTGGAGGAGTGCTTCTTGAGCTATAGTTATCATCCAGATGGGATACCTGAAGATGACCACCCACATGATCGAGAAAACAAAGGCGGAGGAAATGGAGGAAATGGGAAAAAGGTGGCAATTGTTGTGGGAGGAGCAGCTGCTTTGGTTTTTGCCTTCATTTTTCTCTTGTTTGTCAGGTCTTGGTCAAAGAAAGAAGATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

300

Amino Acids

32.01

Weight (kDa)

5.48

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 34 - 133 1.9e-13 Salt stress response/antifungal
Stress-antifung PF01657 160 - 240 1.1e-08 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 528
AccI GTMKAC 2 cut(s) 93, 411
AccII CGCG 1 cut(s) 617
AciI CCGC 9 cut(s) 231, 248, 479, 506, 528, 536, 572, 641, 777
AcuI CTGAAG 2 cut(s) 675, 762
AfaI GTAC 1 cut(s) 645
AgsI TTSAA 2 cut(s) 9, 176
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 5 cut(s) 373, 400, 503, 714, 830
AluI AGCT 5 cut(s) 373, 400, 503, 714, 830
Alw21I GWGCWC 2 cut(s) 393, 402
Alw26I GTCTC 1 cut(s) 228
Alw44I GTGCAC 1 cut(s) 389
AoxI GGCC 1 cut(s) 259
ApaLI GTGCAC 1 cut(s) 389
ApeKI GCWGC 5 cut(s) 373, 503, 515, 827, 830
AseI ATTAAT 1 cut(s) 437
AspLEI GCGC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 2 cut(s) 393, 402
BbvI GCAGC 5 cut(s) 360, 490, 502, 817, 839
BccI CCATC 2 cut(s) 398, 725
BciVI GTATCC 1 cut(s) 729
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 2 cut(s) 182, 426
BfmI CTRYAG 1 cut(s) 715
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 729
BisI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
BlsI GCNGC 8 cut(s) 375, 481, 505, 508, 517, 530, 829, 832
BmsI GCATC 3 cut(s) 35, 62, 194
BpiI GAAGAC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 730
BsaAI YACGTR 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 1 cut(s) 724
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 4 cut(s) 184, 308, 713, 837
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 5 cut(s) 360, 490, 502, 817, 839
Bsh1236I CGCG 1 cut(s) 617
BshFI GGCC 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 393, 402
BsiSI CCGG 2 cut(s) 258, 274
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 393, 402
Bsp143I GATC 1 cut(s) 760
BspACI CCGC 9 cut(s) 231, 248, 479, 506, 528, 536, 572, 641, 777
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 601
BspFNI CGCG 1 cut(s) 617
BspMI ACCTGC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 1 cut(s) 760
BssNAI GTATAC 1 cut(s) 94
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 86, 542
Bst6I CTCTTC 3 cut(s) 269, 324, 651
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 3 cut(s) 219, 229, 275
BstDEI CTNAG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 617
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 1 cut(s) 763
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 1 cut(s) 760
BstMWI GCNNNNNNNGC 6 cut(s) 59, 148, 397, 512, 515, 711
BstSFI CTRYAG 1 cut(s) 715
BstSLI GKGCMC 1 cut(s) 393
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 5 cut(s) 360, 490, 502, 817, 839
BstV2I GAAGAC 1 cut(s) 452
BstZ17I GTATAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 724
BtsIMutI CAGTG 2 cut(s) 275, 596
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 3 cut(s) 219, 229, 275
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 273
Csp6I GTAC 1 cut(s) 644
CspCI CAANNNNNGTGG 4 cut(s) 456, 491, 798, 833
CviAII CATG 2 cut(s) 377, 758
CviQI GTAC 1 cut(s) 644
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 762
DpnII GATC 1 cut(s) 760
Eam1104I CTCTTC 3 cut(s) 269, 324, 651
EarI CTCTTC 3 cut(s) 269, 324, 651
EciI GGCGGA 1 cut(s) 792
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 675, 762
EcoICRI GAGCTC 1 cut(s) 400
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 2 cut(s) 380, 761
FaiI YATR 8 cut(s) 94, 213, 378, 432, 567, 584, 717, 759
FatI CATG 2 cut(s) 376, 757
FauI CCCGC 1 cut(s) 241
FblI GTMKAC 2 cut(s) 93, 411
Fnu4HI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
FokI GGATG 1 cut(s) 711
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
FspBI CTAG 2 cut(s) 182, 426
GlaI GCGC 1 cut(s) 616
GluI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 258, 274
HhaI GCGC 1 cut(s) 617
Hin1II CATG 2 cut(s) 380, 761
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 4 cut(s) 5, 350, 408, 413
HpaII CCGG 2 cut(s) 258, 274
Hpy166II GTNNAC 3 cut(s) 94, 391, 412
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 3 cut(s) 709, 728, 764
Hpy8I GTNNAC 3 cut(s) 94, 391, 412
HpyAV CCTTC 3 cut(s) 432, 451, 856
HpyCH4III ACNGT 2 cut(s) 86, 542
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 7 cut(s) 26, 62, 107, 376, 391, 442, 577
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 148, 397, 512, 515, 711
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 2 cut(s) 380, 761
HspAI GCGC 1 cut(s) 615
KroI GCCGGC 1 cut(s) 273
KroNI GCCGGC 1 cut(s) 275
Kzo9I GATC 1 cut(s) 760
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 5 cut(s) 360, 490, 502, 817, 839
LweI GCATC 3 cut(s) 35, 62, 194
MaeI CTAG 2 cut(s) 182, 426
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 2 cut(s) 299, 323
MalI GATC 1 cut(s) 762
MbiI CCGCTC 1 cut(s) 528
MboI GATC 1 cut(s) 760
MboII GAAGA 6 cut(s) 256, 311, 457, 668, 755, 899
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 2 cut(s) 393, 402
MluCI AATT 2 cut(s) 70, 810
MlyI GAGTC 3 cut(s) 359, 407, 417
MmeI TCCRAC 1 cut(s) 99
MroNI GCCGGC 1 cut(s) 273
MseI TTAA 1 cut(s) 437
MslI CAYNNNNRTG 3 cut(s) 308, 729, 756
MspA1I CMGCKG 3 cut(s) 231, 373, 830
MspI CCGG 2 cut(s) 258, 274
MunI CAATTG 1 cut(s) 810
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 6 cut(s) 59, 148, 397, 512, 515, 711
NaeI GCCGGC 1 cut(s) 275
NdeII GATC 1 cut(s) 760
NgoMIV GCCGGC 1 cut(s) 273
NlaIII CATG 2 cut(s) 380, 761
NmuCI GTSAC 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 408
PdiI GCCGGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 8 cut(s) 375, 481, 505, 508, 517, 530, 829, 832
PleI GAGTC 3 cut(s) 358, 407, 416
PpsI GAGTC 3 cut(s) 358, 407, 416
Ppu21I YACGTR 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 402
PvuII CAGCTG 2 cut(s) 373, 830
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
RseI CAYNNNNRTG 3 cut(s) 308, 729, 756
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
Sau3AI GATC 1 cut(s) 760
SchI GAGTC 3 cut(s) 359, 407, 417
SduI GDGCHC 2 cut(s) 393, 402
SfaNI GCATC 3 cut(s) 35, 62, 194
SfcI CTRYAG 1 cut(s) 715
SmiMI CAYNNNNRTG 3 cut(s) 308, 729, 756
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse9I AATT 2 cut(s) 70, 810
SsiI CCGC 9 cut(s) 231, 248, 479, 506, 528, 536, 572, 641, 777
SspMI CTAG 2 cut(s) 182, 426
SstI GAGCTC 1 cut(s) 402
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 86, 542
TaiI ACGT 1 cut(s) 390
TaqI TCGA 3 cut(s) 402, 411, 763
TasI AATT 2 cut(s) 70, 810
TauI GCSGC 3 cut(s) 482, 509, 531
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 2 cut(s) 275, 596
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 5 cut(s) 373, 503, 515, 827, 830
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 153, 838
TspGWI ACGGA 1 cut(s) 8
TspRI CASTG 2 cut(s) 275, 596
VneI GTGCAC 1 cut(s) 389
VspI ATTAAT 1 cut(s) 437
XmiI GTMKAC 2 cut(s) 93, 411
XspI CTAG 2 cut(s) 182, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.