Rw3G001460

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015642
Physical Location & Seq
Reverse (-)
1234286 .. 1235427
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001460.1

Sequence Viewer

Length: 888 bp
ATGGATTCAACCTCGAAACCATTCTCTCATGTAGTGTTTCTCTGTTTCGCAGCCCTGTTTCTCTCGTCTGTGATATCAATCTCCGATCACAGTTCATTAGTGTACAAAAACTGTGCAAACCAAACATTCACAACCACAACTCCTACTAGTCCTTACACACAAACCCTCAGATCTCTTTTCCAACAACTGACCGCCCACTCAAACCAGTCCAAGTTCTTCAAACACACCGAATCAACAGACGACGATGAGATCTCCGGTGTATACCAATGCCGGGGAGACATCACTAACCAAGAGTGTCACAGCTGCGTGAGCACGCTTCCGGATATCTCCAACACGATGTGCAGAGAGTCCGTGGCGGCTAGGGTTCAGCTCCACGGGTGCTACATGCACTACAAGCTCGAGGGGACCGACACTGACGACTCCAGCAAGGAAAGTTTGCTGCACAAGAGTTGTGGCGAGGGTGGTGGATCAATCAATGGGGGTTCGGGCGGGTTCGAGGAGATGATGAGAGCAGCGTTTGGGGTTTTGGAGAATGGTGTGGTGAGTGGTAAAGGGTTTTTCAAGACGAGGTATGAGTTAGTGGACGTGATGGCTCAGTGTGAAGGAGGGTTGGGGGGTTGTGAATGTGGGGAGTGTGTGAGTAGTGGGGTGCAGATTGCTGAGGAAGAGTGTGGTGGTTCGGTTTCCGGTGAGATTTATTTGGCGCAGTGCTTCTTGAGCTTTGCTTATCATCCAGATGATGAGTCACACCCAGGCCCAGAACAGAAGGACAAGAAAGATAGCAACGGGAAAACGTTAGCAATTGTTGTGGGAGGGGCAGCAGCTTTGTTTTTTGGGCTCATCTTTTTGTTGTTCCTCAAATCTTGGGGAAAGAAAGATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

295

Amino Acids

31.9

Weight (kDa)

5.02

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 132 5.4e-17 Salt stress response/antifungal
Stress-antifung PF01657 163 - 241 1e-05 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 3 cut(s) 192, 356, 489
AclI AACGTT 1 cut(s) 794
AclWI GGATC 1 cut(s) 475
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 3 cut(s) 9, 220, 562
AhlI ACTAGT 1 cut(s) 146
AjiI CACGTC 1 cut(s) 586
AjnI CCWGG 1 cut(s) 751
AluBI AGCT 5 cut(s) 303, 370, 397, 720, 824
AluI AGCT 5 cut(s) 303, 370, 397, 720, 824
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 1 cut(s) 475
Ama87I CYCGRG 1 cut(s) 398
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 6 cut(s) 50, 303, 439, 512, 818, 821
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 2 cut(s) 405, 755
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 553, 701
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 6 cut(s) 62, 290, 426, 524, 830, 833
BccI CCATC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 753
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 3 cut(s) 147, 360, 886
BglII AGATCT 2 cut(s) 170, 249
BisI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 819, 822
BlsI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 820, 823
Bme1390I CCNGG 2 cut(s) 272, 753
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 405, 755
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 2 cut(s) 272, 753
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 271, 351, 373, 751
BsaWI WCCGGW 3 cut(s) 254, 319, 686
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 1 cut(s) 753
BseDI CCNNGG 4 cut(s) 271, 351, 373, 751
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMII CTCAG 3 cut(s) 181, 608, 651
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 6 cut(s) 62, 290, 426, 524, 830, 833
BsgI GTGCAG 3 cut(s) 361, 425, 671
BshFI GGCC 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 4 cut(s) 255, 271, 320, 687
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 1 cut(s) 756
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 314, 840
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 4 cut(s) 85, 170, 249, 467
BspACI CCGC 3 cut(s) 192, 356, 489
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 3 cut(s) 180, 607, 652
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 406
BspPI GGATC 1 cut(s) 475
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 271, 351, 373, 751
BssMI GATC 4 cut(s) 85, 170, 249, 467
BssNAI GTATAC 1 cut(s) 262
Bst1107I GTATAC 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 92, 113
Bst6I CTCTTC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 3 cut(s) 167, 594, 660
BstDSI CCRYGG 2 cut(s) 351, 373
BstF5I GGATG 1 cut(s) 730
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 4 cut(s) 88, 173, 252, 470
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 85, 170, 249, 467
BstMWI GCNNNNNNNGC 3 cut(s) 309, 394, 717
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 270, 751
BstV1I GCAGC 6 cut(s) 62, 290, 426, 524, 830, 833
BstX2I RGATCY 2 cut(s) 170, 249
BstYI RGATCY 2 cut(s) 170, 249
BstZ17I GTATAC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 756
BtgI CCRYGG 2 cut(s) 351, 373
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 730
BtsI GCAGTG 1 cut(s) 713
BtsIMutI CAGTG 3 cut(s) 411, 602, 713
Cac8I GCNNGC 1 cut(s) 314
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 405, 755
Csp6I GTAC 1 cut(s) 103
CspCI CAANNNNNGTGG 4 cut(s) 433, 468, 789, 824
CviAII CATG 2 cut(s) 29, 385
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 3 cut(s) 167, 594, 660
DpnI GATC 4 cut(s) 87, 172, 251, 469
DpnII GATC 4 cut(s) 85, 170, 249, 467
DraIII CACNNNGTG 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 840
Eco32I GATATC 2 cut(s) 75, 325
Eco47I GGWCC 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 751
EcoRV GATATC 2 cut(s) 75, 325
EcoT38I GRGCYC 1 cut(s) 840
FaeI CATG 2 cut(s) 32, 388
FaiI YATR 4 cut(s) 30, 262, 386, 573
FaqI GGGAC 1 cut(s) 418
FatI CATG 2 cut(s) 28, 384
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 819, 822
FokI GGATG 1 cut(s) 717
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 819, 822
FspBI CTAG 3 cut(s) 147, 360, 886
GlaI GCGC 1 cut(s) 705
GluI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 819, 822
GsuI CTGGAG 1 cut(s) 406
HaeIII GGCC 1 cut(s) 756
HapII CCGG 4 cut(s) 255, 271, 320, 687
HhaI GCGC 1 cut(s) 706
Hin1II CATG 2 cut(s) 32, 388
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HinfI GANTC 5 cut(s) 5, 230, 347, 419, 743
HpaII CCGG 4 cut(s) 255, 271, 320, 687
HphI GGTGA 2 cut(s) 553, 701
Hpy166II GTNNAC 3 cut(s) 103, 262, 583
Hpy188I TCNGA 2 cut(s) 85, 170
Hpy188III TCNNGA 4 cut(s) 320, 562, 715, 734
Hpy8I GTNNAC 3 cut(s) 103, 262, 583
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 596, 760
HpyCH4III ACNGT 2 cut(s) 92, 113
HpyCH4IV ACGT 2 cut(s) 585, 794
HpyCH4V TGCA 5 cut(s) 116, 342, 388, 442, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 394, 717
HpyF3I CTNAG 3 cut(s) 167, 594, 660
HpySE526I ACGT 2 cut(s) 585, 794
Hsp92II CATG 2 cut(s) 32, 388
HspAI GCGC 1 cut(s) 704
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 85, 170, 249, 467
LmnI GCTCC 1 cut(s) 375
Lsp1109I GCAGC 6 cut(s) 62, 290, 426, 524, 830, 833
MaeI CTAG 3 cut(s) 147, 360, 886
MaeII ACGT 2 cut(s) 585, 794
MaeIII GTNAC 2 cut(s) 296, 744
MalI GATC 4 cut(s) 87, 172, 251, 469
MboI GATC 4 cut(s) 85, 170, 249, 467
MboII GAAGA 2 cut(s) 208, 677
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 2 cut(s) 170, 249
MhlI GDGCHC 2 cut(s) 314, 840
MluCI AATT 1 cut(s) 801
MlyI GAGTC 3 cut(s) 356, 413, 752
MmeI TCCRAC 2 cut(s) 205, 354
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 4 cut(s) 255, 271, 320, 687
MspR9I CCNGG 2 cut(s) 272, 753
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 1 cut(s) 753
MwoI GCNNNNNNNGC 3 cut(s) 309, 394, 717
NciI CCSGG 1 cut(s) 272
NdeII GATC 4 cut(s) 85, 170, 249, 467
NlaIII CATG 2 cut(s) 32, 388
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 2 cut(s) 296, 744
NspI RCATGY 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 5, 230
PkrI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 820, 823
PleI GAGTC 3 cut(s) 355, 413, 751
PpsI GAGTC 3 cut(s) 355, 413, 751
Psp1406I AACGTT 1 cut(s) 794
Psp6I CCWGG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 2 cut(s) 405, 755
PspXI VCTCGAGB 1 cut(s) 398
PsuI RGATCY 2 cut(s) 170, 249
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 735
SatI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 819, 822
Sau3AI GATC 4 cut(s) 85, 170, 249, 467
Sau96I GGNCC 2 cut(s) 405, 755
SchI GAGTC 3 cut(s) 356, 413, 752
ScrFI CCNGG 2 cut(s) 272, 753
SduI GDGCHC 2 cut(s) 314, 840
SetI ASST 9 cut(s) 14, 305, 372, 399, 572, 588, 722, 797, 826
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 2 cut(s) 398, 715
SmoI CTYRAG 2 cut(s) 398, 715
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 1 cut(s) 801
SsiI CCGC 3 cut(s) 192, 356, 489
SspMI CTAG 3 cut(s) 147, 360, 886
StyD4I CCNGG 2 cut(s) 270, 751
TaaI ACNGT 2 cut(s) 92, 113
TaiI ACGT 2 cut(s) 588, 797
TaqI TCGA 3 cut(s) 14, 399, 495
TaqII GACCGA 1 cut(s) 422
TasI AATT 1 cut(s) 801
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 5, 230
TscAI CASTG 3 cut(s) 418, 602, 713
TseFI GTSAC 2 cut(s) 296, 744
TseI GCWGC 6 cut(s) 50, 303, 439, 512, 818, 821
Tsp45I GTSAC 2 cut(s) 296, 744
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 3 cut(s) 418, 602, 713
VpaK11BI GGWCC 1 cut(s) 405
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 3 cut(s) 147, 360, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.