RLG00000025833

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015642
Physical Location & Seq
Forward (+)
48577854 .. 48578981
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025833

Sequence Viewer

Length: 894 bp
ATGGATTCAACCTCGAAACCATTCTCTCATGTAGTGTTTCTCTGTTTCGCAGCCCTGTTTCTCTCGTCTGTGATATCAATCTCCGATCACAGTTCATTAGTGTACAAAAACTGTGCAAACCAAACATTCACAACCACAACTCCTACTAGTCCTCACACACAAACCCTCAGATCTCTTTTCCAACAACTGACCGCCCACTCAAACCAGTCCAAGTTCTTCAAACACACCGAATCAACAGACGACGATGAGATCTCCGGTGTATACCAATGCCGGGGAGACATCACTAACCAAGAGTGTCACAGCTGCGTGAGCACGCTTCCGGATATCTCCAAGACGATGTGCAGAGAGTCCGTGGCGGCTCGGGTTCAGCTCCACGGGTGCTACATGCACTACAAGCTCGAGGGGACCGACACTGACGACTCCAGCAAGGAAAGGTTGCTGCACAAGAGTTGTGGCGAGGGTGGTGGATCCATCAATGGGGGTTCGGGCGGGTTCGAAGAGATGATGAGAGCAGCGTTTGGGGTTTTGGAGAATGGTGTGGTGAGTGGTGAAGGGTTTTTCAAGACGAGGTATGAGTTAGTGGACGTGATGGCTCAGTGTGAAGGAGGGTTGGGGGGTTGTGAATGTGGGGAGTGTGTGAGTAGTGGGGTGCAGATTGCTGAGGAAGAGTGTGGTGGTTCGGTTTCCGGTGAGATTTATTTGGCGGAGTGCTTCTTGAGCTTTGCTTATCATCCAGATGATGAGTCACACCCACGCCCAGATGACGAACAGAAGGACAAGAAAGATAGCAACGGGAAAACGTTGGCAATTGTTGTGGGAGGGGCAGCAGCTTTGTGTTTTGGGCTCATCTTTTTGTTGTTCCTCAGATCTTGGGGAAAGAAAGATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

298

Amino Acids

32.27

Weight (kDa)

4.99

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 132 5.1e-16 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 4 cut(s) 192, 356, 489, 704
AclI AACGTT 1 cut(s) 800
AclWI GGATC 2 cut(s) 462, 475
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 271, 477
AgsI TTSAA 3 cut(s) 9, 220, 562
AhlI ACTAGT 1 cut(s) 146
AjiI CACGTC 1 cut(s) 586
AluBI AGCT 5 cut(s) 303, 370, 397, 720, 830
AluI AGCT 5 cut(s) 303, 370, 397, 720, 830
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 462, 475
Ama87I CYCGRG 2 cut(s) 360, 398
Aor13HI TCCGGA 1 cut(s) 319
ApeKI GCWGC 6 cut(s) 50, 303, 439, 512, 824, 827
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 553, 560, 701
AsuII TTCGAA 1 cut(s) 495
AvaI CYCGRG 2 cut(s) 360, 398
AvaII GGWCC 1 cut(s) 405
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BccI CCATC 2 cut(s) 479, 583
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 2 cut(s) 147, 892
BglII AGATCT 3 cut(s) 170, 249, 866
BisI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
BlsI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 826, 829
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 2 cut(s) 360, 398
BmgBI CACGTC 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 2 cut(s) 406, 469
BmrFI CCNGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 271, 351, 373
BsaWI WCCGGW 3 cut(s) 254, 319, 686
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 477
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 271, 351, 373
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 271, 477
BseMII CTCAG 4 cut(s) 181, 608, 651, 877
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BsgI GTGCAG 3 cut(s) 361, 425, 671
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 2 cut(s) 360, 398
BsiSI CCGG 4 cut(s) 255, 271, 320, 687
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 2 cut(s) 271, 477
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 418
BsoBI CYCGRG 2 cut(s) 360, 398
Bsp119I TTCGAA 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 314, 846
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 5 cut(s) 85, 170, 249, 467, 866
BspACI CCGC 4 cut(s) 192, 356, 489, 704
BspCNI CTCAG 4 cut(s) 180, 607, 652, 876
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 406, 469
BspPI GGATC 2 cut(s) 462, 475
BspT104I TTCGAA 1 cut(s) 495
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 271, 351, 373
BssMI GATC 5 cut(s) 85, 170, 249, 467, 866
BssNAI GTATAC 1 cut(s) 262
Bst1107I GTATAC 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 92, 113
Bst6I CTCTTC 2 cut(s) 492, 660
BstAUI TGTACA 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 4 cut(s) 167, 594, 660, 863
BstDSI CCRYGG 2 cut(s) 351, 373
BstF5I GGATG 1 cut(s) 730
BstKTI GATC 5 cut(s) 88, 173, 252, 470, 869
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 5 cut(s) 85, 170, 249, 467, 866
BstMWI GCNNNNNNNGC 3 cut(s) 309, 394, 717
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BstX2I RGATCY 4 cut(s) 170, 249, 467, 866
BstYI RGATCY 4 cut(s) 170, 249, 467, 866
BstZ17I GTATAC 1 cut(s) 262
BtgI CCRYGG 2 cut(s) 351, 373
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 2 cut(s) 411, 602
Cac8I GCNNGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 1 cut(s) 103
CspCI CAANNNNNGTGG 4 cut(s) 433, 468, 795, 830
CviAII CATG 2 cut(s) 29, 385
CviJI RGCY 9 cut(s) 53, 303, 359, 370, 397, 593, 720, 830, 844
CviKI_1 RGCY 9 cut(s) 53, 303, 359, 370, 397, 593, 720, 830, 844
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 4 cut(s) 167, 594, 660, 863
DpnI GATC 5 cut(s) 87, 172, 251, 469, 868
DpnII GATC 5 cut(s) 85, 170, 249, 467, 866
Eam1104I CTCTTC 2 cut(s) 492, 660
EarI CTCTTC 2 cut(s) 492, 660
EciI GGCGGA 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 846
Eco32I GATATC 2 cut(s) 75, 325
Eco47I GGWCC 1 cut(s) 405
Eco88I CYCGRG 2 cut(s) 360, 398
EcoRV GATATC 2 cut(s) 75, 325
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 2 cut(s) 32, 388
FaiI YATR 4 cut(s) 30, 262, 386, 573
FaqI GGGAC 1 cut(s) 418
FatI CATG 2 cut(s) 28, 384
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
FokI GGATG 1 cut(s) 717
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
FspBI CTAG 2 cut(s) 147, 892
GluI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
GsuI CTGGAG 1 cut(s) 406
HapII CCGG 4 cut(s) 255, 271, 320, 687
Hin1II CATG 2 cut(s) 32, 388
HinfI GANTC 5 cut(s) 5, 230, 347, 419, 743
HpaII CCGG 4 cut(s) 255, 271, 320, 687
HphI GGTGA 3 cut(s) 553, 560, 701
Hpy166II GTNNAC 3 cut(s) 103, 262, 583
Hpy188I TCNGA 3 cut(s) 85, 170, 866
Hpy188III TCNNGA 4 cut(s) 320, 562, 715, 734
Hpy8I GTNNAC 3 cut(s) 103, 262, 583
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 545, 596, 766
HpyCH4III ACNGT 2 cut(s) 92, 113
HpyCH4IV ACGT 2 cut(s) 585, 800
HpyCH4V TGCA 5 cut(s) 116, 342, 388, 442, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 394, 717
HpyF3I CTNAG 4 cut(s) 167, 594, 660, 863
HpySE526I ACGT 2 cut(s) 585, 800
Hsp92II CATG 2 cut(s) 32, 388
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 85, 170, 249, 467, 866
LmnI GCTCC 1 cut(s) 375
LpnPI CCDG 9 cut(s) 68, 218, 268, 284, 333, 436, 700, 747, 771
Lsp1109I GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
MaeI CTAG 2 cut(s) 147, 892
MaeII ACGT 2 cut(s) 585, 800
MaeIII GTNAC 2 cut(s) 296, 744
MalI GATC 5 cut(s) 87, 172, 251, 469, 868
MboI GATC 5 cut(s) 85, 170, 249, 467, 866
MboII GAAGA 3 cut(s) 208, 509, 677
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 4 cut(s) 170, 249, 467, 866
MhlI GDGCHC 2 cut(s) 314, 846
MluCI AATT 1 cut(s) 807
MlyI GAGTC 3 cut(s) 356, 413, 752
MmeI TCCRAC 1 cut(s) 205
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 4 cut(s) 255, 271, 320, 687
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 807
MwoI GCNNNNNNNGC 3 cut(s) 309, 394, 717
NciI CCSGG 1 cut(s) 272
NdeII GATC 5 cut(s) 85, 170, 249, 467, 866
NlaIII CATG 2 cut(s) 32, 388
NlaIV GGNNCC 2 cut(s) 406, 469
NmuCI GTSAC 2 cut(s) 296, 744
NspI RCATGY 1 cut(s) 388
NspV TTCGAA 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 5, 230
PkrI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 826, 829
PleI GAGTC 3 cut(s) 355, 413, 751
PpsI GAGTC 3 cut(s) 355, 413, 751
Psp1406I AACGTT 1 cut(s) 800
PspN4I GGNNCC 2 cut(s) 406, 469
PspPI GGNCC 1 cut(s) 405
PspXI VCTCGAGB 1 cut(s) 398
PsuI RGATCY 4 cut(s) 170, 249, 467, 866
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 735
SatI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
Sau3AI GATC 5 cut(s) 85, 170, 249, 467, 866
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 3 cut(s) 356, 413, 752
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 2 cut(s) 314, 846
Sfr274I CTCGAG 1 cut(s) 398
SfuI TTCGAA 1 cut(s) 495
SinI GGWCC 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 2 cut(s) 398, 715
SmoI CTYRAG 2 cut(s) 398, 715
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 1 cut(s) 807
SsiI CCGC 4 cut(s) 192, 356, 489, 704
SspMI CTAG 2 cut(s) 147, 892
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 92, 113
TaiI ACGT 2 cut(s) 588, 803
TaqI TCGA 3 cut(s) 14, 399, 495
TaqII GACCGA 1 cut(s) 422
TasI AATT 1 cut(s) 807
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 5, 230
TscAI CASTG 2 cut(s) 418, 602
TseFI GTSAC 2 cut(s) 296, 744
TseI GCWGC 6 cut(s) 50, 303, 439, 512, 824, 827
Tsp45I GTSAC 2 cut(s) 296, 744
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 2 cut(s) 418, 602
VpaK11BI GGWCC 1 cut(s) 405
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 2 cut(s) 147, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.