pycom04g20550

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015642
Physical Location & Seq
Forward (+)
22020946 .. 22023196
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20550.2

Sequence Viewer

Length: 903 bp
ATGGATTCAACCTTTCTCCGTTTTGCATCGCTACTACTTCTACTTTCTCTCGCATCATCTGCAAACCCTAATTCCGACTACAACAGTTTGGTATACAAGAAATGTGCAAATCAAACATTTACAGCTCTCCCAACTAAATCGCAAGTCCCACAACAAACCCTTTCATCTTTTTTTCAACAACTGACATCACACTCCTCCCAATCAAAGTTCTATAAGCACGCCGAAGCCAGCGGAGACAACAACGAGGCGGGTATTTCCGGCCTCTTCCAGTGCCGGCAGGACATCAGCAATGAGGAGTGTCACACCTGCGTGAACACCCTTCGTAACTTATCGAACACCTTGTGCAAAGAGTCGGTTTCGGCTCGTGTTCAGCTGCATGGGTGCTACGTGCGCTACGAGCTCGATGGAGTCGACTCAAAGCCCCCCAGCCATAGATTAATGCAGAAGACTTGTGGGAAGGATTTGGCAAATGGGGTGGCGGCAGGGTTTGAGAAAATGAGAGCTGCGGCGTTTGCTGCCTTGGAGAGCGGCGTTGCGGATGGTGGTGGGTTTTGCCAAACGAGTTATGAGGCGGTGCAAGTTATGGCTCAGTGTGTCGGGGGCTTGGGGGGTTGCGTGTGTGGAGAGTGTGTGAGTAGGGCGGTACAAAGTGCTGAAGAAGAATGTGGTGGTGCTGTTTCTGGGCAGATTTATTTTGAGGAGTGCTTCTTGAGCTATAGTTATCATCCAGATGGGATACCTGCAGATGACCACCCAGATGATCGAAAAAACAAAGGTGGAGGAAATGGGGGAAGTGGGAAAAAGGTGGCAATTCTTGTGGGAGGAGCAGCTGCTTTGGCTTTTGCCTTCATTTTTCTCTTGTTCGTCAGGTCTTGGTCAAAGAAAGAAGATGACTGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

301

Amino Acids

32.08

Weight (kDa)

5.67

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 2 cut(s) 314, 748
AccBSI CCGCTC 1 cut(s) 528
AccI GTMKAC 2 cut(s) 93, 411
AciI CCGC 8 cut(s) 231, 248, 479, 506, 528, 536, 572, 641
AcuI CTGAAG 1 cut(s) 675
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 1 cut(s) 645
AgsI TTSAA 2 cut(s) 9, 176
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 6 cut(s) 125, 373, 400, 503, 714, 830
AluI AGCT 6 cut(s) 125, 373, 400, 503, 714, 830
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 228
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 5 cut(s) 373, 503, 515, 827, 830
AseI ATTAAT 1 cut(s) 437
AspLEI GCGC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 5 cut(s) 360, 490, 502, 817, 839
BccI CCATC 3 cut(s) 398, 533, 725
BciVI GTATCC 1 cut(s) 729
BcoDI GTCTC 1 cut(s) 228
BfmI CTRYAG 2 cut(s) 715, 741
BfuAI ACCTGC 2 cut(s) 314, 748
BfuI GTATCC 1 cut(s) 729
BisI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
BlsI GCNGC 8 cut(s) 375, 481, 505, 508, 517, 530, 829, 832
BmsI GCATC 2 cut(s) 35, 62
BpiI GAAGAC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 730
BsaAI YACGTR 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 2 cut(s) 544, 724
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 4 cut(s) 184, 308, 713, 837
BseXI GCAGC 5 cut(s) 360, 490, 502, 817, 839
BseYI CCCAGC 1 cut(s) 425
BshFI GGCC 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 2 cut(s) 258, 274
BslFI GGGAC 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 1 cut(s) 760
BspACI CCGC 8 cut(s) 231, 248, 479, 506, 528, 536, 572, 641
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 601
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 2 cut(s) 314, 748
BsrBI CCGCTC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 1 cut(s) 760
BssNAI GTATAC 1 cut(s) 94
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 86, 896
Bst6I CTCTTC 1 cut(s) 269
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 3 cut(s) 219, 229, 275
BstDEI CTNAG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 544, 724
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 1 cut(s) 763
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 1 cut(s) 760
BstMWI GCNNNNNNNGC 7 cut(s) 59, 390, 397, 512, 515, 711, 836
BstSFI CTRYAG 2 cut(s) 715, 741
BstV1I GCAGC 5 cut(s) 360, 490, 502, 817, 839
BstV2I GAAGAC 1 cut(s) 452
BstZ17I GTATAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 544, 724
BtsIMutI CAGTG 2 cut(s) 275, 596
BveI ACCTGC 2 cut(s) 314, 748
Cac8I GCNNGC 3 cut(s) 219, 229, 275
CfoI GCGC 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 273
Csp6I GTAC 1 cut(s) 644
CspCI CAANNNNNGTGG 4 cut(s) 456, 491, 798, 833
CviAII CATG 1 cut(s) 377
CviQI GTAC 1 cut(s) 644
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 762
DpnII GATC 1 cut(s) 760
DraIII CACNNNGTG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 675
EcoICRI GAGCTC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 1 cut(s) 380
FaiI YATR 7 cut(s) 94, 213, 378, 432, 567, 584, 717
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 376
FauI CCCGC 1 cut(s) 241
FblI GTMKAC 2 cut(s) 93, 411
Fnu4HI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
FokI GGATG 2 cut(s) 551, 711
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
GlaI GCGC 1 cut(s) 392
GluI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 258, 274
HhaI GCGC 1 cut(s) 393
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 4 cut(s) 5, 350, 408, 413
HpaII CCGG 2 cut(s) 258, 274
Hpy166II GTNNAC 3 cut(s) 94, 313, 412
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 709, 728
Hpy8I GTNNAC 3 cut(s) 94, 313, 412
HpyAV CCTTC 3 cut(s) 329, 451, 856
HpyCH4III ACNGT 2 cut(s) 86, 896
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 8 cut(s) 26, 62, 107, 345, 376, 442, 577, 743
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 390, 397, 512, 515, 711, 836
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 1 cut(s) 391
KroI GCCGGC 1 cut(s) 273
KroNI GCCGGC 1 cut(s) 275
Kzo9I GATC 1 cut(s) 760
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 5 cut(s) 360, 490, 502, 817, 839
LweI GCATC 2 cut(s) 35, 62
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 299, 323, 896
MalI GATC 1 cut(s) 762
MbiI CCGCTC 1 cut(s) 528
MboI GATC 1 cut(s) 760
MboII GAAGA 5 cut(s) 256, 457, 668, 671, 899
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 70, 810
MlyI GAGTC 3 cut(s) 359, 407, 417
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 8 cut(s) 205, 238, 272, 286, 562, 691, 773, 815
MroNI GCCGGC 1 cut(s) 273
MseI TTAA 1 cut(s) 437
MslI CAYNNNNRTG 3 cut(s) 308, 729, 756
MspA1I CMGCKG 3 cut(s) 231, 373, 830
MspI CCGG 2 cut(s) 258, 274
MwoI GCNNNNNNNGC 7 cut(s) 59, 390, 397, 512, 515, 711, 836
NaeI GCCGGC 1 cut(s) 275
NdeII GATC 1 cut(s) 760
NgoMIV GCCGGC 1 cut(s) 273
NlaIII CATG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 299, 896
OliI CACNNNNGTG 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 408
PdiI GCCGGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 8 cut(s) 375, 481, 505, 508, 517, 530, 829, 832
PleI GAGTC 3 cut(s) 358, 407, 416
PpsI GAGTC 3 cut(s) 358, 407, 416
Ppu21I YACGTR 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 425
PstI CTGCAG 1 cut(s) 745
PvuII CAGCTG 2 cut(s) 373, 830
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
RseI CAYNNNNRTG 3 cut(s) 308, 729, 756
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 8 cut(s) 374, 480, 504, 507, 516, 529, 828, 831
Sau3AI GATC 1 cut(s) 760
SchI GAGTC 3 cut(s) 359, 407, 417
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 2 cut(s) 35, 62
SfcI CTRYAG 2 cut(s) 715, 741
SmiMI CAYNNNNRTG 3 cut(s) 308, 729, 756
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse9I AATT 2 cut(s) 70, 810
SsiI CCGC 8 cut(s) 231, 248, 479, 506, 528, 536, 572, 641
SstI GAGCTC 1 cut(s) 402
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 86, 896
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 332, 402, 411, 763
TasI AATT 2 cut(s) 70, 810
TauI GCSGC 3 cut(s) 482, 509, 531
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 2 cut(s) 275, 596
TseFI GTSAC 2 cut(s) 299, 896
TseI GCWGC 5 cut(s) 373, 503, 515, 827, 830
Tsp45I GTSAC 2 cut(s) 299, 896
TspDTI ATGAA 2 cut(s) 153, 838
TspGWI ACGGA 1 cut(s) 8
TspRI CASTG 2 cut(s) 275, 596
VspI ATTAAT 1 cut(s) 437
XmiI GTMKAC 2 cut(s) 93, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.