Rroxscaffold_6G00427370

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015642
Physical Location & Seq
Reverse (-)
48007929 .. 48009131
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427370.1

Sequence Viewer

Length: 777 bp
ATGGATTCAACCTCTAAACCACTCTCTCATGCAGTGTTTCTCTGTTTCGGAGCCCTGTTTCTCTCGTCTGTGATATCAATCTCCGATCACAGTTCATTAGTGTACAAAAACTGTGCAAACCAAACATTCACAACCACAGCTCCCACTAGTCCTCACACACAGACCCTCAGATCTCTTTTCCAAGAACTGACCGCCCACTCAAACCAGTCCAAGTTCTTCAAACACACCGAATCAACAGACGACGATGAGATCTCCGGTGTATACCAATGCCGGGGAGACATCACTAACCAAGAGTGTCACAGCTGCGTGAGCACGCTTCCGGATATCTCCAACACCATGTGCAGAGAATCCGTGGCGGCTAGGGTTCAGCTCCACGGGTGCTACATGCACTACAAGCTTGAGGGGATCGACACTGACGACTCCAGCAAGGAAAGGTTGCTGCACAAGAGTTGCGGCGAGGGTGGTGGATCCATAAATGGGGGTTCGGGCGGGTTCGAGGAGATGATGAGAGCAGCGTTTGGGGTTTTGGAGAATGGTGTGTGTGTGAGTAGTGGGGTGCAGATTGCTGAGGAAGAGTGTGGTGGTTCGGTTTCCGGTGAGATTTATTTGGCGGAGTGCTTCTTGAGCTTTGCTTATCATCCAGATGAAGAACAGAAGGGCAAGAAAGATAGCAACGGGAAAACGTTAGCAATTGTTGTGGGAGGGGCAGCAGCTTTGTGTTTTGGGCTCATCTTTTTGTTGTTCCTCAAATCTTGGGGAAAGAAAGATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

258

Amino Acids

27.91

Weight (kDa)

5.23

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 132 8.2e-16 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 5 cut(s) 192, 356, 453, 489, 611
AclI AACGTT 1 cut(s) 683
AclWI GGATC 3 cut(s) 413, 462, 475
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 271, 477
AgsI TTSAA 2 cut(s) 9, 220
AhlI ACTAGT 1 cut(s) 146
AluBI AGCT 6 cut(s) 140, 303, 370, 397, 627, 713
AluI AGCT 6 cut(s) 140, 303, 370, 397, 627, 713
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 3 cut(s) 413, 462, 475
Aor13HI TCCGGA 1 cut(s) 319
ApeKI GCWGC 5 cut(s) 303, 439, 512, 707, 710
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 608
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 55, 729
Bbv12I GWGCWC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 290, 426, 524, 719, 722
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 3 cut(s) 147, 360, 775
BglII AGATCT 2 cut(s) 170, 249
BisI GCNGC 7 cut(s) 304, 357, 440, 454, 513, 708, 711
BlsI GCNGC 7 cut(s) 305, 358, 441, 455, 514, 709, 712
Bme1390I CCNGG 1 cut(s) 272
BmiI GGNNCC 2 cut(s) 52, 469
BmrFI CCNGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 419, 643
BpuMI CCSGG 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 271, 351, 373
BsaWI WCCGGW 3 cut(s) 254, 319, 593
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 477
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 319
BseDI CCNNGG 3 cut(s) 271, 351, 373
BseGI GGATG 1 cut(s) 637
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 271, 477
BseMII CTCAG 2 cut(s) 181, 558
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 5 cut(s) 290, 426, 524, 719, 722
BsgI GTGCAG 3 cut(s) 361, 425, 578
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 4 cut(s) 255, 271, 320, 594
BslI CCNNNNNNNGG 2 cut(s) 271, 477
BsmAI GTCTC 1 cut(s) 270
Bsp1286I GDGCHC 3 cut(s) 55, 314, 729
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 5 cut(s) 85, 170, 249, 405, 467
BspACI CCGC 5 cut(s) 192, 356, 453, 489, 611
BspCNI CTCAG 2 cut(s) 180, 559
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 52, 469
BspPI GGATC 3 cut(s) 413, 462, 475
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 271, 351, 373
BssMI GATC 5 cut(s) 85, 170, 249, 405, 467
BssNAI GTATAC 1 cut(s) 262
Bst1107I GTATAC 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 92, 113
Bst6I CTCTTC 1 cut(s) 567
BstAUI TGTACA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 2 cut(s) 167, 567
BstDSI CCRYGG 2 cut(s) 351, 373
BstF5I GGATG 1 cut(s) 637
BstKTI GATC 5 cut(s) 88, 173, 252, 408, 470
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 5 cut(s) 85, 170, 249, 405, 467
BstMWI GCNNNNNNNGC 3 cut(s) 309, 394, 624
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 5 cut(s) 290, 426, 524, 719, 722
BstX2I RGATCY 3 cut(s) 170, 249, 467
BstYI RGATCY 3 cut(s) 170, 249, 467
BstZ17I GTATAC 1 cut(s) 262
BtgI CCRYGG 2 cut(s) 351, 373
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 39, 411
Cac8I GCNNGC 1 cut(s) 314
Csp6I GTAC 1 cut(s) 103
CspCI CAANNNNNGTGG 2 cut(s) 678, 713
CviAII CATG 3 cut(s) 29, 337, 385
CviJI RGCY 9 cut(s) 53, 140, 303, 359, 370, 397, 627, 713, 727
CviKI_1 RGCY 9 cut(s) 53, 140, 303, 359, 370, 397, 627, 713, 727
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 167, 567
DpnI GATC 5 cut(s) 87, 172, 251, 407, 469
DpnII GATC 5 cut(s) 85, 170, 249, 405, 467
DraIII CACNNNGTG 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
EciI GGCGGA 1 cut(s) 626
Eco24I GRGCYC 2 cut(s) 55, 729
Eco32I GATATC 2 cut(s) 75, 325
EcoRV GATATC 2 cut(s) 75, 325
EcoT38I GRGCYC 2 cut(s) 55, 729
FaeI CATG 3 cut(s) 32, 340, 388
FaiI YATR 5 cut(s) 30, 262, 338, 386, 473
FatI CATG 3 cut(s) 28, 336, 384
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 7 cut(s) 304, 357, 440, 454, 513, 708, 711
FokI GGATG 1 cut(s) 624
FriOI GRGCYC 2 cut(s) 55, 729
Fsp4HI GCNGC 7 cut(s) 304, 357, 440, 454, 513, 708, 711
FspBI CTAG 3 cut(s) 147, 360, 775
GluI GCNGC 7 cut(s) 304, 357, 440, 454, 513, 708, 711
GsuI CTGGAG 1 cut(s) 406
HapII CCGG 4 cut(s) 255, 271, 320, 594
Hin1II CATG 3 cut(s) 32, 340, 388
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 4 cut(s) 5, 230, 347, 419
HpaII CCGG 4 cut(s) 255, 271, 320, 594
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 2 cut(s) 103, 262
Hpy188I TCNGA 3 cut(s) 50, 85, 170
Hpy188III TCNNGA 3 cut(s) 320, 622, 641
Hpy8I GTNNAC 2 cut(s) 103, 262
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 2 cut(s) 92, 113
HpyCH4IV ACGT 1 cut(s) 683
HpyCH4V TGCA 6 cut(s) 32, 116, 342, 388, 442, 559
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 394, 624
HpyF3I CTNAG 2 cut(s) 167, 567
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 3 cut(s) 32, 340, 388
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 85, 170, 249, 405, 467
LmnI GCTCC 3 cut(s) 50, 145, 375
LpnPI CCDG 8 cut(s) 68, 218, 268, 284, 333, 436, 607, 654
Lsp1109I GCAGC 5 cut(s) 290, 426, 524, 719, 722
MaeI CTAG 3 cut(s) 147, 360, 775
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 1 cut(s) 296
MalI GATC 5 cut(s) 87, 172, 251, 407, 469
MboI GATC 5 cut(s) 85, 170, 249, 405, 467
MboII GAAGA 3 cut(s) 208, 584, 659
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 3 cut(s) 170, 249, 467
MhlI GDGCHC 3 cut(s) 55, 314, 729
MluCI AATT 1 cut(s) 690
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 9 cut(s) 22, 162, 176, 394, 451, 490, 562, 695, 755
MroI TCCGGA 1 cut(s) 319
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 4 cut(s) 255, 271, 320, 594
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 690
MwoI GCNNNNNNNGC 3 cut(s) 309, 394, 624
NciI CCSGG 1 cut(s) 272
NdeII GATC 5 cut(s) 85, 170, 249, 405, 467
NlaIII CATG 3 cut(s) 32, 340, 388
NlaIV GGNNCC 2 cut(s) 52, 469
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 3 cut(s) 5, 230, 347
PkrI GCNGC 7 cut(s) 305, 358, 441, 455, 514, 709, 712
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 683
PspN4I GGNNCC 2 cut(s) 52, 469
PsuI RGATCY 3 cut(s) 170, 249, 467
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 642
SatI GCNGC 7 cut(s) 304, 357, 440, 454, 513, 708, 711
Sau3AI GATC 5 cut(s) 85, 170, 249, 405, 467
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 55, 314, 729
SetI ASST 9 cut(s) 14, 142, 305, 372, 399, 437, 629, 686, 715
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 398, 622
SmoI CTYRAG 2 cut(s) 398, 622
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 1 cut(s) 690
SsiI CCGC 5 cut(s) 192, 356, 453, 489, 611
SspMI CTAG 3 cut(s) 147, 360, 775
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 92, 113
TaiI ACGT 1 cut(s) 686
TaqI TCGA 2 cut(s) 408, 495
TasI AATT 1 cut(s) 690
TatI WGTACW 1 cut(s) 102
TauI GCSGC 2 cut(s) 359, 456
TfiI GAWTC 3 cut(s) 5, 230, 347
TscAI CASTG 2 cut(s) 39, 418
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 5 cut(s) 303, 439, 512, 707, 710
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 84, 660
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 2 cut(s) 39, 418
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 3 cut(s) 147, 360, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.