RchiOBHm_Chr3g0449411

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015642
Physical Location & Seq
Reverse (-)
1209212 .. 1210353
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41677

Sequence Viewer

Length: 894 bp
ATGGATTCAACCTCGAAACCATTCTCTCATGTAGTGTTTCTCTGTTTCGCAGCCCTGTTTCTCTCGTCTGTGATATCAATCTCCGATCACAGTTCATTAGTGTACAAAAACTGTGCAAACCAAACATTCACAACCACAACTCCTACTAGTCCTCACACACAAACCCTCAGATCTCTTTTCCAACAACTGACCGCCCACTCAAACCAGTCCAAGTTCTTCAAACACACCGAATCAACAGACGACGATGAGATCTCCGGTGTATACCAATGCCGGGGAGACATCACTAACCAAGAGTGTCACAGCTGCGTGAGCACGCTTCCGGATATCTCCAACACGATGTGCAGAGAGTCCGTGGCGGCCAGGGTTCAGCTCCACGGGTGCTACATGCACTACAAGCTCGAGGGGACCGACACTGACGACTCCAGCAAGGAAAGTTTGCTGCACAAGAGTTGTGGCGAGGGTGGTGGATCCATCAATGGGGGTCCGGGCGGGTTCGAGGAGATGATGAGAGCAGCGTTTGGGGTTTTGGAGAATGGTGTGGTGAGTGGTAAAGGGTTTTTCAAGACGAGGTATGAGTTAGTGGACGTGATGGCTCAGTGTGAAGGAGGGTTGGGGGGTTGTGAATGTGGGGAGTGTGTGAGTAGTGGGGTGCAGATTGCTGAGGAAGAGTGTGGTGGTTCGGTTTCCGGTGAGATTTATTTGGCGGAGTGCTTCTTGAGCTTTGCTTATCATCCAGATGATGAGTCACACCCAGGCCCAGGTGAAGAACAGAAGGACAAGAAAGATAGCAACGGGAAAACGTTAGCAATTGTTGTGGGAGGGGCAGCAGCTTTGTTTTTTGGGCTCATCTTTTTGTTGTTCCTCAAATCTTGGGGAAAGAAAGATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

297

Amino Acids

32.07

Weight (kDa)

4.98

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 132 7e-16 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01600
malus_domestica MD00G1149100.v1.1 MD04G1232100.v1.1
prunus_persica Prupe.6G351500_v2.0.a1
pyrus_communis pycom04g20550
rosa_chinensis RchiOBHm_Chr3g0449411
rosa_laevigata RLG00000025833
rosa_multiflora Rmu_sc0019012.1_g000009 Rmu_sc0020017.1_g000002
rosa_roxburghii Rroxscaffold_6G00427370
rosa_rugosa Rorug02G0620400
rosa_samantha Rh3AG018200 Rh3BG018500 Rh3CG017400 Rh3DG018900
rosa_wichuraiana Rw3G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 4 cut(s) 192, 356, 489, 704
AclI AACGTT 1 cut(s) 800
AclWI GGATC 2 cut(s) 462, 475
AcoI YGGCCR 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 3 cut(s) 271, 477, 758
AgsI TTSAA 3 cut(s) 9, 220, 562
AhlI ACTAGT 1 cut(s) 146
AjiI CACGTC 1 cut(s) 586
AjnI CCWGG 3 cut(s) 359, 751, 757
AluBI AGCT 5 cut(s) 303, 370, 397, 720, 830
AluI AGCT 5 cut(s) 303, 370, 397, 720, 830
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 462, 475
Ama87I CYCGRG 1 cut(s) 398
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 2 cut(s) 357, 754
ApeKI GCWGC 6 cut(s) 50, 303, 439, 512, 824, 827
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 3 cut(s) 405, 482, 755
AsuC2I CCSGG 2 cut(s) 272, 486
AsuHPI GGTGA 3 cut(s) 553, 701, 773
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 2 cut(s) 405, 482
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BccI CCATC 2 cut(s) 479, 583
BciT130I CCWGG 3 cut(s) 361, 753, 759
BcnI CCSGG 2 cut(s) 272, 486
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 2 cut(s) 147, 892
BglII AGATCT 2 cut(s) 170, 249
BisI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
BlsI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 826, 829
Bme1390I CCNGG 5 cut(s) 272, 361, 486, 753, 759
Bme18I GGWCC 2 cut(s) 405, 482
BmeT110I CYCGRG 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 405, 482, 755
BmiI GGNNCC 3 cut(s) 406, 469, 483
BmrFI CCNGG 5 cut(s) 272, 361, 486, 753, 759
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 272, 486
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 6 cut(s) 271, 351, 360, 373, 751, 757
BsaWI WCCGGW 3 cut(s) 254, 319, 686
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 477, 758
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 3 cut(s) 361, 753, 759
BseDI CCNNGG 6 cut(s) 271, 351, 360, 373, 751, 757
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 3 cut(s) 271, 477, 758
BseMII CTCAG 3 cut(s) 181, 608, 651
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BsgI GTGCAG 3 cut(s) 361, 425, 671
BshFI GGCC 2 cut(s) 359, 756
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 5 cut(s) 255, 271, 320, 485, 687
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 3 cut(s) 271, 477, 758
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 2 cut(s) 359, 756
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 314, 846
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 4 cut(s) 85, 170, 249, 467
BspACI CCGC 4 cut(s) 192, 356, 489, 704
BspANI GGCC 2 cut(s) 359, 756
BspCNI CTCAG 3 cut(s) 180, 607, 652
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 406, 469, 483
BspPI GGATC 2 cut(s) 462, 475
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 6 cut(s) 271, 351, 360, 373, 751, 757
BssMI GATC 4 cut(s) 85, 170, 249, 467
BssNAI GTATAC 1 cut(s) 262
Bst1107I GTATAC 1 cut(s) 262
Bst2UI CCWGG 3 cut(s) 361, 753, 759
Bst4CI ACNGT 2 cut(s) 92, 113
Bst6I CTCTTC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 3 cut(s) 167, 594, 660
BstDSI CCRYGG 2 cut(s) 351, 373
BstF5I GGATG 1 cut(s) 730
BstKTI GATC 4 cut(s) 88, 173, 252, 470
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 85, 170, 249, 467
BstMWI GCNNNNNNNGC 3 cut(s) 309, 394, 717
BstNI CCWGG 3 cut(s) 361, 753, 759
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 5 cut(s) 270, 359, 484, 751, 757
BstV1I GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
BstX2I RGATCY 3 cut(s) 170, 249, 467
BstYI RGATCY 3 cut(s) 170, 249, 467
BstZ17I GTATAC 1 cut(s) 262
BsuRI GGCC 2 cut(s) 359, 756
BtgI CCRYGG 2 cut(s) 351, 373
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 2 cut(s) 411, 602
Cac8I GCNNGC 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 405, 482, 755
Csp6I GTAC 1 cut(s) 103
CspCI CAANNNNNGTGG 4 cut(s) 433, 468, 795, 830
CviAII CATG 2 cut(s) 29, 385
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 3 cut(s) 167, 594, 660
DpnI GATC 4 cut(s) 87, 172, 251, 469
DpnII GATC 4 cut(s) 85, 170, 249, 467
DraIII CACNNNGTG 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 846
Eco32I GATATC 2 cut(s) 75, 325
Eco47I GGWCC 2 cut(s) 405, 482
Eco88I CYCGRG 1 cut(s) 398
EcoRII CCWGG 3 cut(s) 359, 751, 757
EcoRV GATATC 2 cut(s) 75, 325
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 2 cut(s) 32, 388
FaiI YATR 4 cut(s) 30, 262, 386, 573
FaqI GGGAC 1 cut(s) 418
FatI CATG 2 cut(s) 28, 384
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
FokI GGATG 1 cut(s) 717
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
FspBI CTAG 2 cut(s) 147, 892
GluI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
GsuI CTGGAG 1 cut(s) 406
HaeIII GGCC 2 cut(s) 359, 756
HapII CCGG 5 cut(s) 255, 271, 320, 485, 687
Hin1II CATG 2 cut(s) 32, 388
HinfI GANTC 5 cut(s) 5, 230, 347, 419, 743
HpaII CCGG 5 cut(s) 255, 271, 320, 485, 687
HphI GGTGA 3 cut(s) 553, 701, 773
Hpy166II GTNNAC 3 cut(s) 103, 262, 583
Hpy188I TCNGA 2 cut(s) 85, 170
Hpy188III TCNNGA 4 cut(s) 320, 562, 715, 734
Hpy8I GTNNAC 3 cut(s) 103, 262, 583
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 596, 766
HpyCH4III ACNGT 2 cut(s) 92, 113
HpyCH4IV ACGT 2 cut(s) 585, 800
HpyCH4V TGCA 5 cut(s) 116, 342, 388, 442, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 394, 717
HpyF3I CTNAG 3 cut(s) 167, 594, 660
HpySE526I ACGT 2 cut(s) 585, 800
Hsp92II CATG 2 cut(s) 32, 388
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 85, 170, 249, 467
LmnI GCTCC 1 cut(s) 375
Lsp1109I GCAGC 6 cut(s) 62, 290, 426, 524, 836, 839
MaeI CTAG 2 cut(s) 147, 892
MaeII ACGT 2 cut(s) 585, 800
MaeIII GTNAC 2 cut(s) 296, 744
MalI GATC 4 cut(s) 87, 172, 251, 469
MboI GATC 4 cut(s) 85, 170, 249, 467
MboII GAAGA 3 cut(s) 208, 677, 776
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 3 cut(s) 170, 249, 467
MhlI GDGCHC 2 cut(s) 314, 846
MluCI AATT 1 cut(s) 807
MlyI GAGTC 3 cut(s) 356, 413, 752
MmeI TCCRAC 2 cut(s) 205, 354
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 5 cut(s) 255, 271, 320, 485, 687
MspR9I CCNGG 5 cut(s) 272, 361, 486, 753, 759
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 3 cut(s) 361, 753, 759
MwoI GCNNNNNNNGC 3 cut(s) 309, 394, 717
NciI CCSGG 2 cut(s) 272, 486
NdeII GATC 4 cut(s) 85, 170, 249, 467
NlaIII CATG 2 cut(s) 32, 388
NlaIV GGNNCC 3 cut(s) 406, 469, 483
NmuCI GTSAC 2 cut(s) 296, 744
NspI RCATGY 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 5, 230
PkrI GCNGC 7 cut(s) 52, 305, 358, 441, 514, 826, 829
PleI GAGTC 3 cut(s) 355, 413, 751
PpsI GAGTC 3 cut(s) 355, 413, 751
Psp1406I AACGTT 1 cut(s) 800
Psp6I CCWGG 3 cut(s) 359, 751, 757
PspGI CCWGG 3 cut(s) 359, 751, 757
PspN4I GGNNCC 3 cut(s) 406, 469, 483
PspPI GGNCC 3 cut(s) 405, 482, 755
PspXI VCTCGAGB 1 cut(s) 398
PsuI RGATCY 3 cut(s) 170, 249, 467
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 735
SatI GCNGC 7 cut(s) 51, 304, 357, 440, 513, 825, 828
Sau3AI GATC 4 cut(s) 85, 170, 249, 467
Sau96I GGNCC 3 cut(s) 405, 482, 755
SchI GAGTC 3 cut(s) 356, 413, 752
ScrFI CCNGG 5 cut(s) 272, 361, 486, 753, 759
SduI GDGCHC 2 cut(s) 314, 846
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 2 cut(s) 405, 482
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 2 cut(s) 398, 715
SmoI CTYRAG 2 cut(s) 398, 715
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 1 cut(s) 807
SsiI CCGC 4 cut(s) 192, 356, 489, 704
SspMI CTAG 2 cut(s) 147, 892
StyD4I CCNGG 5 cut(s) 270, 359, 484, 751, 757
TaaI ACNGT 2 cut(s) 92, 113
TaiI ACGT 2 cut(s) 588, 803
TaqI TCGA 3 cut(s) 14, 399, 495
TaqII GACCGA 1 cut(s) 422
TasI AATT 1 cut(s) 807
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 5, 230
TscAI CASTG 2 cut(s) 418, 602
TseFI GTSAC 2 cut(s) 296, 744
TseI GCWGC 6 cut(s) 50, 303, 439, 512, 824, 827
Tsp45I GTSAC 2 cut(s) 296, 744
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 2 cut(s) 418, 602
VpaK11BI GGWCC 2 cut(s) 405, 482
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 2 cut(s) 147, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.