MD04G1235300.v1.1

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010024
Physical Location & Seq
Forward (+)
31432347 .. 31440952
8606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1235300.v1.1.491

Sequence Viewer

Length: 2985 bp
ATGATCACGGTTAAGGGAGAGGCTCGTCAGCCGCCGGCGGTGGTCGGAGCTTCTAGGGCTCACTTCGGCGTCGCTCGCGATGACACCTTTGGGATCAAGCCGGAGCCGTCGTCGCTCAGCTTGGTCTCTTTCAAGCCTCATCACGAGGCTCGTGAAGTTGATGAAGACGCACATTTGACTGTTGCTCATCAAATGTACAAGGCTGGAAACTATAAGGAGGCACTAGAGCATAGCAAAATCGTGTATGAGAAAAACCCGATTCGCACCGACAATCTCCTGCTTTTGGGTGCAATCTATTATCAGTTGCATGACTTTGATATGTGTATTGCCAAAAATGAAGAAGCCCTTCGAATTGAGCCCCATTTCGCCGAGTGTTATGGGAACATGGCTAATGCTTGGAAGGAAAAAGGAAATAATGACCTTGCTATTCGCTATTATTTGGTTGCCATTGAGCTTCGTCCCAACTTCTGTGATGCATGGTCAAACTTAGCTAGTGCATACATGCGGAAAGGAAGACATGAAGAAGCAGCACAGTGTTGTCGCCAGGCACTTGCATTAAATCCCCATTTGGTTGATGCCCATAGTAATCTTGGAAATCTAATGAAGGCACGGGGATTGGTGCAAGAGGCATACAGTTGCTACCTTGAGGCTATACGTATACAACCACACTTCGCAATTGCATGGTCTAATCTTGCTGGTCTTTTCATGGAGTCGGGTGATCTTAATAGAGCCCTGCAGTACTATAAGGAGGCTGTGAAACTCAAACCTGCTTTTCCAGATGCGTATCTCAACCTTGGGAATGTTTATAAGGCTTTGGGACTGCCTCAGGAGGCAATCGTGTGTTATCAGCGTGCTCTCCAGACAAGACCCAACTATGCAATGGCTTTCGGAAATTTAGCTAGTTCGTATTATGAGCAAGGTCAACTGGATCTGGCAATCCTACATTATAAGCAAGCCATTTCCTGTGATGCAAGATTTTTGGAGGCGTACAATAATTTGGGCAATGCTCTGAAAGACATTGGCAGAGTGGATGAAGCAATTCAATGCTACAACCAATGCCTTACTTTACAACCTAACCATCCACAAGCGCTTACAAACCTTGGGAATATTTATATGGAATGGAACATGGTGGCTGCTGCTGCTCAATATTATAAGGCCACATTGACCGTAACAACTGGATTGTCTGCTCCTTTTAATAATCTTGCCATCATCTATAAGCAACAGGGGAACTATGCGGATGCAATTTCTTGCTACAATGAGGTTCTTCGCATTGATCCCTTGGCAGCTGATGGGCTTGTCAATAGGGGCAACACATACAAAGAGATTGGTAGAGTCAGTGAAGCAATTCAGGACTACATACATGCAATATCTGTCCGGCCAACCATGGCTGAAGCTCATGCAAACTTGGCTTCAGCTTACAAGGATAGTGGACATGTTGAGGCTGCTATTAAGAGCTATAGTCAGGCATTGCTTCTTCGAACTGATTTTCCAGAGGCAACTTGTAATCTTTTACATACATTACAGTGTGTATGCAATTGGGAGGATCGTGACAAAATGTTTTCTGAAGTGGAAGGCATAATCAGGAGGCAGATTAATATGTCTCTTCTGCCTAGTGTGCAACCTTTCCACGCAATAGCATATCCTATTGATCCAATTCTGGCACTTGAAATTAGGAATTATTTTTCTTCTCATTCACAAGTTTGTTCTCTCTTTCAAAGTCGTAAATATGCTGCACACTGCTCCATAGTTGCATCCCGATTTGGACTTTCTCCCTTCAACCATCCTGCTCCTGTCCCCATAAGGCATAATGGAGGACCTCAGAGACTAAGGGTTGGATATGTAAGCAGTGACTTTGGTAATCACCCTTTGTCACATCTTATGGGATCTGTGTTTGGGATGCATAACAAGGACAATGTTGAGGTTTTCTGTTATGCCTTGAGTCCAAATGATGGAACGGAATGGAGACAACGCATCCAGTCTGAAGCAGAGCACTTTATCGATGTCTCTTCTTTGTCATCAGATATGATTGCCAAAATGATTAATGAAGATAAGATACAAGTGTTAATTAACCTCAATGGCTATACAAAGGGGGCAAGAAATGAGATATTTGCTATGCAGCCAGCTCCCATTCAGGTTTCATACATGGGGTTCCCTGGTACCACGGGGGCAAATTACATCGATTATCTAGTCACTGATGAGTTCGTTTCACCTTTACGCTACGCACATATTTATTCTGAGAAAATTGTTCATCTCCCTCATTGCTACTTTGTGAATGATTATAAGCAGAAAAATCAAGATGTGTTGGATCCAAGCTGCCGCCACAAGCGATCGGATTATGGGCTGCCCGAGGATAAATTTATATTCGCATGCTTCAATCAGTTGTACAAAATGGATCCTGAAATTTTTAATACTTGGTGCAATATTCTTAAATGTGTACCCAACAGTGCACTTTGGCTCCTCAGATTCCCAGCTGCAGGCGAAACGAGACTTCGTAAATATGCTGTTGATCAAGGAGTCCAGCCAGACCAAATCATCTTCACGGATGTTGCTATGAAAGGTGAACATATCAGGCGGAGTGCGTTGGCAGATTTATTCCTTGACACGCCTTTGTGCAATGCACATACGACAGGCACGGATATTCTATGGGCAGGTCTGCCGATGGTGACCCTTCCCCTTGAAAAGATGGCTACCAGGGTTGCTGGATCACTGTGTCTCGCCACTGGACTGGGAGATGAGATGATTGTTAGCAGCATGAAAGAGTATGAAGAGAAGGCAGTGTCTCTTTCGTTGAATCCTCCAAAGCTCCGTGCTCTTGCCAACAAGCTCAAGGCAGCTCGACTGACTTGTCCTCTGTTCGACACTGCACGCTGGGTGAGGAATCTGGAGAGGAGTTACTTCAAAATGTGGAATCTGCATTGCTCGGGCCAGAAGCCGCAGCATTTCAAAGTCACCGAGAACGATTTAGAGTTCCCCTACGATAGATAG

Protein Analysis

995

Amino Acids

112.04

Weight (kDa)

6.59

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_11 PF13414 131 - 171 4.6e-06 TPR repeat
TPR_16 PF13432 135 - 188 2.4e-06 Tetratricopeptide repeat
TPR_1 PF00515 158 - 189 1.2e-09 Tetratricopeptide repeat
TPR_8 PF13181 158 - 188 4.1e-06 Tetratricopeptide repeat
TPR_2 PF07719 158 - 189 2.3e-08 Tetratricopeptide repeat
TPR_11 PF13414 165 - 203 1.4e-07 TPR repeat
TPR_1 PF00515 225 - 256 9.2e-06 Tetratricopeptide repeat
TPR_11 PF13414 235 - 273 5.8e-11 TPR repeat
TPR_19 PF14559 236 - 292 3.2e-08 Tetratricopeptide repeat
TPR_1 PF00515 259 - 292 7.5e-10 Tetratricopeptide repeat
TPR_2 PF07719 259 - 292 5.6e-06 Tetratricopeptide repeat
TPR_12 PF13424 261 - 321 6.4e-10 Tetratricopeptide repeat
TPR_16 PF13432 263 - 322 3.6e-06 Tetratricopeptide repeat
TPR_11 PF13414 302 - 341 5.2e-09 TPR repeat
TPR_12 PF13424 327 - 389 4.3e-07 Tetratricopeptide repeat
TPR_2 PF07719 328 - 360 4.3e-07 Tetratricopeptide repeat
TPR_8 PF13181 328 - 360 2.9e-08 Tetratricopeptide repeat
TPR_1 PF00515 328 - 360 1.4e-09 Tetratricopeptide repeat
TPR_11 PF13414 334 - 372 1.2e-08 TPR repeat
TPR_1 PF00515 398 - 426 6.8e-08 Tetratricopeptide repeat
TPR_2 PF07719 399 - 426 1.6e-06 Tetratricopeptide repeat
TPR_9 PF13371 403 - 466 3.1e-06 Tetratricopeptide repeat
TPR_11 PF13414 404 - 443 1.7e-07 TPR repeat
TPR_11 PF13414 436 - 477 2.7e-07 TPR repeat
Glyco_transf_41 PF13844 510 - 763 1.5e-79 Glycosyl transferase family 41
Glyco_transf_41 PF13844 775 - 961 1.6e-67 Glycosyl transferase family 41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 807, 948, 1152, 2280
AasI GACNNNNNNGTC 1 cut(s) 2844
Acc36I ACCTGC 2 cut(s) 775, 2639
Acc65I GGTACC 1 cut(s) 2156
AccB1I GGYRCC 1 cut(s) 2156
AccB7I CCANNNNNTGG 1 cut(s) 1949
AccI GTMKAC 1 cut(s) 658
AccII CGCG 1 cut(s) 78
AciI CCGC 7 cut(s) 32, 38, 505, 1235, 2317, 2572, 2933
AcoI YGGCCR 1 cut(s) 1376
AcsI RAATTY 3 cut(s) 892, 2354, 2400
AcuI CTGAAG 4 cut(s) 1395, 1410, 1584, 2001
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 6 cut(s) 197, 740, 989, 2158, 2384, 2436
AfeI AGCGCT 1 cut(s) 1089
AfiI CCNNNNNNNGG 3 cut(s) 283, 1949, 2474
AflIII ACRYGT 1 cut(s) 1432
AjnI CCWGG 3 cut(s) 543, 2152, 2690
AloI GAACNNNNNNTCC 2 cut(s) 1471, 1503
Alw21I GWGCWC 4 cut(s) 856, 1992, 2449, 2812
Alw26I GTCTC 8 cut(s) 130, 1605, 1816, 1957, 2008, 2479, 2717, 2784
Alw44I GTGCAC 1 cut(s) 2445
Ama87I CYCGRG 2 cut(s) 2345, 2920
Aor51HI AGCGCT 1 cut(s) 1089
AoxI GGCC 3 cut(s) 1155, 1376, 2923
ApaLI GTGCAC 1 cut(s) 2445
ApoI RAATTY 3 cut(s) 892, 2354, 2400
AseI ATTAAT 2 cut(s) 1593, 2040
Asp700I GAANNNNTTC 3 cut(s) 345, 1038, 1344
Asp718I GGTACC 1 cut(s) 2156
AspLEI GCGC 1 cut(s) 1090
AspS9I GGNCC 2 cut(s) 1814, 2923
AsuHPI GGTGA 7 cut(s) 728, 1853, 2199, 2570, 2674, 2884, 2941
AsuII TTCGAA 2 cut(s) 349, 1477
AvaI CYCGRG 2 cut(s) 2345, 2920
AvaII GGWCC 1 cut(s) 1814
AxyI CCTNAGG 1 cut(s) 825
BaeGI GKGCMC 1 cut(s) 2449
BaeI ACNNNNGTAYC 2 cut(s) 2045, 2078
BamHI GGATCC 2 cut(s) 2305, 2392
BanI GGYRCC 1 cut(s) 2156
BanII GRGCYC 3 cut(s) 61, 360, 733
BarI GAAGNNNNNNTAC 2 cut(s) 2037, 2069
BauI CACGAG 2 cut(s) 143, 150
BbsI GAAGAC 2 cut(s) 171, 520
Bbv12I GWGCWC 4 cut(s) 856, 1992, 2449, 2812
BccI CCATC 7 cut(s) 1086, 1214, 1283, 1788, 1943, 2653, 2677
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 545, 2154, 2692
BclI TGATCA 2 cut(s) 3, 2506
BcoDI GTCTC 8 cut(s) 130, 1605, 1816, 1957, 2008, 2479, 2717, 2784
BfaI CTAG 6 cut(s) 54, 224, 492, 900, 1611, 2186
BfmI CTRYAG 3 cut(s) 734, 1456, 2472
BfoI RGCGCY 1 cut(s) 1091
BfuAI ACCTGC 2 cut(s) 775, 2639
BlpI GCTNAGC 1 cut(s) 116
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 3 cut(s) 545, 2154, 2692
Bme18I GGWCC 1 cut(s) 1814
BmeT110I CYCGRG 2 cut(s) 2345, 2920
BmgT120I GGNCC 2 cut(s) 1814, 2923
BmiI GGNNCC 6 cut(s) 105, 2150, 2158, 2307, 2394, 2456
BmrFI CCNGG 3 cut(s) 545, 2154, 2692
BmrI ACTGGG 1 cut(s) 2735
BmsI GCATC 8 cut(s) 463, 565, 769, 958, 1228, 1760, 1887, 1980
BmuI ACTGGG 1 cut(s) 2735
BpiI GAAGAC 2 cut(s) 171, 520
BpmI CTGGAG 2 cut(s) 842, 2903
Bpu1102I GCTNAGC 1 cut(s) 116
Bpu14I TTCGAA 2 cut(s) 349, 1477
BpuEI CTTGAG 3 cut(s) 665, 1957, 2810
Bsa29I ATCGAT 2 cut(s) 1998, 2178
BsaAI YACGTR 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 8 cut(s) 793, 1099, 1278, 1383, 2152, 2160, 2346, 2691
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 2439, 2469
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 1949, 2474
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 5 cut(s) 930, 1180, 1975, 2725, 2730
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 6 cut(s) 885, 1009, 1466, 2257, 2620, 2914
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 3 cut(s) 545, 2154, 2692
BseCI ATCGAT 2 cut(s) 1998, 2178
BseDI CCNNGG 8 cut(s) 793, 1099, 1278, 1383, 2152, 2160, 2346, 2691
BseGI GGATG 8 cut(s) 1036, 1078, 1243, 1751, 1780, 1902, 1971, 2548
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 283, 1949, 2474
BseMI GCAATG 6 cut(s) 885, 1009, 1466, 2257, 2620, 2914
BseMII CTCAG 5 cut(s) 130, 839, 1832, 2226, 2473
BseNI ACTGG 5 cut(s) 930, 1180, 1975, 2725, 2730
BseRI GAGGAG 2 cut(s) 2447, 2902
BseSI GKGCMC 1 cut(s) 2449
BseYI CCCAGC 2 cut(s) 2467, 2868
BsgI GTGCAG 2 cut(s) 1716, 2847
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 2330
BshFI GGCC 3 cut(s) 1157, 1378, 2925
BshNI GGYRCC 1 cut(s) 2156
BshVI ATCGAT 2 cut(s) 1998, 2178
BsiEI CGRYCG 1 cut(s) 2330
BsiHKAI GWGCWC 4 cut(s) 856, 1992, 2449, 2812
BsiHKCI CYCGRG 2 cut(s) 2345, 2920
BsiSI CCGG 3 cut(s) 35, 101, 1375
BslFI GGGAC 3 cut(s) 444, 831, 1778
BslI CCNNNNNNNGG 3 cut(s) 283, 1949, 2474
BsmAI GTCTC 8 cut(s) 130, 1605, 1816, 1957, 2008, 2479, 2717, 2784
BsmFI GGGAC 3 cut(s) 444, 831, 1778
BsnI GGCC 3 cut(s) 1157, 1378, 2925
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 2345, 2920
Bsp119I TTCGAA 2 cut(s) 349, 1477
Bsp1286I GDGCHC 7 cut(s) 61, 360, 733, 856, 1992, 2449, 2812
Bsp1407I TGTACA 2 cut(s) 195, 2382
Bsp1720I GCTNAGC 1 cut(s) 116
Bsp19I CCATGG 1 cut(s) 1383
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 7 cut(s) 32, 38, 505, 1235, 2317, 2572, 2933
BspANI GGCC 3 cut(s) 1157, 1378, 2925
BspCNI CTCAG 5 cut(s) 129, 838, 1831, 2227, 2472
BspDI ATCGAT 2 cut(s) 1998, 2178
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 6 cut(s) 105, 2150, 2158, 2307, 2394, 2456
BspMAI CTGCAG 2 cut(s) 738, 2476
BspMI ACCTGC 2 cut(s) 775, 2639
BspT104I TTCGAA 2 cut(s) 349, 1477
BspT107I GGYRCC 1 cut(s) 2156
BspTNI GGTCTC 1 cut(s) 130
BsrDI GCAATG 6 cut(s) 885, 1009, 1466, 2257, 2620, 2914
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 2 cut(s) 195, 2382
BsrI ACTGG 5 cut(s) 930, 1180, 1975, 2725, 2730
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 8 cut(s) 793, 1099, 1278, 1383, 2152, 2160, 2346, 2691
BssNAI GTATAC 1 cut(s) 659
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 2 cut(s) 143, 150
BssT1I CCWWGG 4 cut(s) 793, 1099, 1278, 1383
Bst1107I GTATAC 1 cut(s) 659
Bst2BI CACGAG 2 cut(s) 143, 150
Bst2UI CCWGG 3 cut(s) 545, 2154, 2692
Bst4CI ACNGT 8 cut(s) 10, 181, 534, 635, 1168, 1524, 2444, 2709
Bst6I CTCTTC 3 cut(s) 1608, 2011, 2760
BstACI GRCGYC 1 cut(s) 69
BstAUI TGTACA 2 cut(s) 195, 2382
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 2 cut(s) 349, 1477
BstC8I GCNNGC 8 cut(s) 36, 76, 852, 954, 2121, 2368, 2476, 2866
BstDEI CTNAG 7 cut(s) 116, 487, 825, 1818, 1826, 2235, 2459
BstDSI CCRYGG 2 cut(s) 1383, 2160
BstEII GGTNACC 1 cut(s) 2662
BstF5I GGATG 8 cut(s) 1036, 1078, 1243, 1751, 1780, 1902, 1971, 2548
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 1091
BstHHI GCGC 1 cut(s) 1090
BstMAI GTCTC 8 cut(s) 130, 1605, 1816, 1957, 2008, 2479, 2717, 2784
BstMCI CGRYCG 1 cut(s) 2330
BstMWI GCNNNNNNNGC 6 cut(s) 56, 75, 112, 1139, 1406, 1615
BstNI CCWGG 3 cut(s) 545, 2154, 2692
BstNSI RCATGY 4 cut(s) 505, 1364, 1436, 2370
BstPI GGTNACC 1 cut(s) 2662
BstSCI CCNGG 3 cut(s) 543, 2152, 2690
BstSFI CTRYAG 3 cut(s) 734, 1456, 2472
BstSLI GKGCMC 1 cut(s) 2449
BstSNI TACGTA 1 cut(s) 656
BstUI CGCG 1 cut(s) 78
BstV2I GAAGAC 2 cut(s) 171, 520
BstX2I RGATCY 4 cut(s) 928, 1883, 2305, 2392
BstXI CCANNNNNNTGG 1 cut(s) 2725
BstYI RGATCY 4 cut(s) 928, 1883, 2305, 2392
BstZ17I GTATAC 1 cut(s) 659
Bsu15I ATCGAT 2 cut(s) 1998, 2178
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 3 cut(s) 1157, 1378, 2925
BsuTUI ATCGAT 2 cut(s) 1998, 2178
BtgI CCRYGG 2 cut(s) 1383, 2160
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 8 cut(s) 1036, 1078, 1243, 1751, 1780, 1902, 1971, 2548
BtsI GCAGTG 4 cut(s) 1735, 1852, 2781, 2859
BtuMI TCGCGA 1 cut(s) 78
BveI ACCTGC 2 cut(s) 775, 2639
Cac8I GCNNGC 8 cut(s) 36, 76, 852, 954, 2121, 2368, 2476, 2866
CfoI GCGC 1 cut(s) 1090
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 1814, 2923
ClaI ATCGAT 2 cut(s) 1998, 2178
CseI GACGC 2 cut(s) 58, 176
Csp6I GTAC 6 cut(s) 196, 739, 988, 2157, 2383, 2435
CspCI CAANNNNNGTGG 2 cut(s) 1408, 1443
CviQI GTAC 6 cut(s) 196, 739, 988, 2157, 2383, 2435
DdeI CTNAG 7 cut(s) 116, 487, 825, 1818, 1826, 2235, 2459
DrdI GACNNNNNNGTC 1 cut(s) 2844
DseDI GACNNNNNNGTC 1 cut(s) 2844
EaeI YGGCCR 1 cut(s) 1376
Eam1104I CTCTTC 3 cut(s) 1608, 2011, 2760
EarI CTCTTC 3 cut(s) 1608, 2011, 2760
EciI GGCGGA 1 cut(s) 2587
Eco105I TACGTA 1 cut(s) 656
Eco130I CCWWGG 4 cut(s) 793, 1099, 1278, 1383
Eco24I GRGCYC 3 cut(s) 61, 360, 733
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 1814
Eco47III AGCGCT 1 cut(s) 1089
Eco57I CTGAAG 4 cut(s) 1395, 1410, 1584, 2001
Eco81I CCTNAGG 1 cut(s) 825
Eco88I CYCGRG 2 cut(s) 2345, 2920
Eco91I GGTNACC 1 cut(s) 2662
EcoO109I RGGNCCY 1 cut(s) 1814
EcoO65I GGTNACC 1 cut(s) 2662
EcoRII CCWGG 3 cut(s) 543, 2152, 2690
EcoT14I CCWWGG 4 cut(s) 793, 1099, 1278, 1383
EcoT22I ATGCAT 2 cut(s) 478, 1902
EcoT38I GRGCYC 3 cut(s) 61, 360, 733
ErhI CCWWGG 4 cut(s) 793, 1099, 1278, 1383
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 3 cut(s) 444, 831, 1778
FbaI TGATCA 2 cut(s) 3, 2506
FblI GTMKAC 1 cut(s) 658
FokI GGATG 8 cut(s) 1043, 1065, 1250, 1738, 1767, 1909, 1958, 2555
FriOI GRGCYC 3 cut(s) 61, 360, 733
FspBI CTAG 6 cut(s) 54, 224, 492, 900, 1611, 2186
GlaI GCGC 1 cut(s) 1089
GsaI CCCAGC 2 cut(s) 2471, 2872
GsuI CTGGAG 2 cut(s) 842, 2903
HaeII RGCGCY 1 cut(s) 1091
HaeIII GGCC 3 cut(s) 1157, 1378, 2925
HapII CCGG 3 cut(s) 35, 101, 1375
HgaI GACGC 2 cut(s) 58, 176
HhaI GCGC 1 cut(s) 1090
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HincII GTYRAC 1 cut(s) 923
HindII GTYRAC 1 cut(s) 923
HinfI GANTC 9 cut(s) 259, 710, 1332, 1939, 2463, 2514, 2791, 2878, 2908
HpaII CCGG 3 cut(s) 35, 101, 1375
HphI GGTGA 7 cut(s) 728, 1853, 2199, 2570, 2674, 2884, 2941
Hpy166II GTNNAC 6 cut(s) 659, 923, 1430, 2435, 2447, 2561
Hpy8I GTNNAC 6 cut(s) 659, 923, 1430, 2435, 2447, 2561
Hpy99I CGWCG 3 cut(s) 74, 112, 115
HpyAV CCTTC 7 cut(s) 356, 394, 598, 1565, 1783, 2678, 2764
HpyCH4III ACNGT 8 cut(s) 10, 181, 534, 635, 1168, 1524, 2444, 2709
HpyCH4IV ACGT 1 cut(s) 655
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 75, 112, 1139, 1406, 1615
HpyF3I CTNAG 7 cut(s) 116, 487, 825, 1818, 1826, 2235, 2459
HpySE526I ACGT 1 cut(s) 655
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 1 cut(s) 1088
KpnI GGTACC 1 cut(s) 2160
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 2 cut(s) 3, 2506
LmnI GCTCC 8 cut(s) 47, 103, 1192, 1745, 1792, 2128, 2460, 2808
LweI GCATC 8 cut(s) 463, 565, 769, 958, 1228, 1760, 1887, 1980
MaeI CTAG 6 cut(s) 54, 224, 492, 900, 1611, 2186
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 8 cut(s) 1168, 1547, 1847, 1869, 2188, 2662, 2891, 2947
MfeI CAATTG 2 cut(s) 675, 1534
MflI RGATCY 4 cut(s) 928, 1883, 2305, 2392
MhlI GDGCHC 7 cut(s) 61, 360, 733, 856, 1992, 2449, 2812
MlyI GAGTC 4 cut(s) 719, 1341, 1948, 2523
MmeI TCCRAC 3 cut(s) 25, 1813, 2283
Mph1103I ATGCAT 2 cut(s) 478, 1902
MreI CGCCGGCG 1 cut(s) 34
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 3 cut(s) 345, 1038, 1344
MslI CAYNNNNRTG 1 cut(s) 1522
MspA1I CMGCKG 2 cut(s) 1286, 2471
MspI CCGG 3 cut(s) 35, 101, 1375
MspR9I CCNGG 3 cut(s) 545, 2154, 2692
MunI CAATTG 2 cut(s) 675, 1534
MvaI CCWGG 3 cut(s) 545, 2154, 2692
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 6 cut(s) 56, 75, 112, 1139, 1406, 1615
NaeI GCCGGC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 1383
NgoMIV GCCGGC 1 cut(s) 34
NlaIV GGNNCC 6 cut(s) 105, 2150, 2158, 2307, 2394, 2456
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 6 cut(s) 1547, 1847, 1869, 2188, 2662, 2947
NruI TCGCGA 1 cut(s) 78
NsiI ATGCAT 2 cut(s) 478, 1902
NspI RCATGY 4 cut(s) 505, 1364, 1436, 2370
NspV TTCGAA 2 cut(s) 349, 1477
PacI TTAATTAA 1 cut(s) 2067
PaeI GCATGC 1 cut(s) 2370
PciI ACATGT 1 cut(s) 1432
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 3 cut(s) 345, 1038, 1344
PfeI GAWTC 5 cut(s) 259, 2463, 2791, 2878, 2908
PflMI CCANNNNNTGG 1 cut(s) 1949
Ple19I CGATCG 1 cut(s) 2330
PleI GAGTC 4 cut(s) 718, 1340, 1947, 2522
PpsI GAGTC 4 cut(s) 718, 1340, 1947, 2522
Ppu21I YACGTR 1 cut(s) 656
PpuMI RGGWCCY 1 cut(s) 1814
PscI ACATGT 1 cut(s) 1432
PshBI ATTAAT 2 cut(s) 1593, 2040
PsiI TTATAA 4 cut(s) 807, 948, 1152, 2280
Psp5II RGGWCCY 1 cut(s) 1814
Psp6I CCWGG 3 cut(s) 543, 2152, 2690
PspEI GGTNACC 1 cut(s) 2662
PspFI CCCAGC 2 cut(s) 2467, 2868
PspGI CCWGG 3 cut(s) 543, 2152, 2690
PspN4I GGNNCC 6 cut(s) 105, 2150, 2158, 2307, 2394, 2456
PspPI GGNCC 2 cut(s) 1814, 2923
PspPPI RGGWCCY 1 cut(s) 1814
PstI CTGCAG 2 cut(s) 738, 2476
PsuI RGATCY 4 cut(s) 928, 1883, 2305, 2392
PvuI CGATCG 1 cut(s) 2330
PvuII CAGCTG 2 cut(s) 1286, 2471
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 6 cut(s) 197, 740, 989, 2158, 2384, 2436
RsaNI GTAC 6 cut(s) 196, 739, 988, 2157, 2383, 2435
RseI CAYNNNNRTG 1 cut(s) 1522
Sau96I GGNCC 2 cut(s) 1814, 2923
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 4 cut(s) 719, 1341, 1948, 2523
ScrFI CCNGG 3 cut(s) 545, 2154, 2692
SduI GDGCHC 7 cut(s) 61, 360, 733, 856, 1992, 2449, 2812
SfaNI GCATC 8 cut(s) 463, 565, 769, 958, 1228, 1760, 1887, 1980
SfcI CTRYAG 3 cut(s) 734, 1456, 2472
SfuI TTCGAA 2 cut(s) 349, 1477
SgrAI CRCCGGYG 1 cut(s) 34
SinI GGWCC 1 cut(s) 1814
SmiMI CAYNNNNRTG 1 cut(s) 1522
SmlI CTYRAG 3 cut(s) 644, 1936, 2825
SmoI CTYRAG 3 cut(s) 644, 1936, 2825
SnaBI TACGTA 1 cut(s) 656
SphI GCATGC 1 cut(s) 2370
SsiI CCGC 7 cut(s) 32, 38, 505, 1235, 2317, 2572, 2933
SspI AATATT 3 cut(s) 1108, 1148, 2422
SspMI CTAG 6 cut(s) 54, 224, 492, 900, 1611, 2186
StyD4I CCNGG 3 cut(s) 543, 2152, 2690
StyI CCWWGG 4 cut(s) 793, 1099, 1278, 1383
TaaI ACNGT 8 cut(s) 10, 181, 534, 635, 1168, 1524, 2444, 2709
TaiI ACGT 1 cut(s) 658
TaqI TCGA 6 cut(s) 349, 1477, 1998, 2178, 2836, 2856
TatI WGTACW 3 cut(s) 195, 738, 2382
TauI GCSGC 3 cut(s) 34, 2319, 2935
TfiI GAWTC 5 cut(s) 259, 2463, 2791, 2878, 2908
TseFI GTSAC 6 cut(s) 1547, 1847, 1869, 2188, 2662, 2947
Tsp45I GTSAC 6 cut(s) 1547, 1847, 1869, 2188, 2662, 2947
TspGWI ACGGA 4 cut(s) 1970, 2555, 2648, 2795
Van91I CCANNNNNTGG 1 cut(s) 1949
VneI GTGCAC 1 cut(s) 2445
VpaK11BI GGWCC 1 cut(s) 1814
VspI ATTAAT 2 cut(s) 1593, 2040
XapI RAATTY 3 cut(s) 892, 2354, 2400
XceI RCATGY 4 cut(s) 505, 1364, 1436, 2370
XcmI CCANNNNNNNNNTGG 2 cut(s) 587, 877
XmiI GTMKAC 1 cut(s) 658
XmnI GAANNNNTTC 3 cut(s) 345, 1038, 1344
XspI CTAG 6 cut(s) 54, 224, 492, 900, 1611, 2186
ZrmI AGTACT 1 cut(s) 740
Zsp2I ATGCAT 2 cut(s) 478, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.