MD12G1253100.v1.1

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010024
Physical Location & Seq
Forward (+)
32197466 .. 32207274
9809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1253100.v1.1.491

Sequence Viewer

Length: 2943 bp
ATGATGATCACGGTTCAGGGCGAGGCTCGTCAGCCGCCGGCGGTGGTCGGAGCTTCTAGGGCACACTTCGGCGCCGCTCGCGATGATTCCTTTGGAATTAAGCCGGAGCCGTCGTCGCTCAGCTTGGTCTCTTTCAAGCCTCATCACGATGCTCGTGAAGTTGATGAAGACGCACATATGACTATTGCTCATCAAATGTACAAGGCTGGAAACTATAAGGAGGCTCTAGAGCATAGCAAAATTGTGTATGAGAAAACCCCGATTCGCACCGACAATCTCCTTCTTCTGGGTGCAATCTATTATCAGTTGCATGACTTTGATATGTGTATTGCCAAAAATGAAGAAGCCCTTCGAATTGAGCCGCATTTTGCCGAGTGTTATGGGAACATGGCTAATGCTTGGAAGGAAAAAGGAAATAATGACCTTGCTATTCGCTATTATTTGGTTGCCATTGAGCTTCGTCCGAATTTCTGTGATGCATGGTCAAACTTAGCTAGTGCATACATGCGGAAAGGAAGACATGAAGAAGCGGCACAGTGTTGTCGCCAGGCACTTGCATTAAATCCCCATTTGGTTGATGCCCATAGTAATCTTGGGAATCTAATGAAGGCACGGGGATTGGTGCAAGAGGCATACAGTTGCTACCTCGAGGCTATACGTATCCAACCAAACTTCGCTATTGCATGGTCTAATCTTGCTGGTCTTTTCATGGAGTCTGGTGATCTTAATAGGGCCCTGAAGTACTATAAGGAGGCCGTGAAACTCAAACCTGCTTTTCCAGATGCGTATCTCAATCTTGGGAATGTTTATAAGGCTTTGGGACTGCCACAGGAGGCAATCGTGTGTTATCAACGTGCTCTCCAGACAAGACCTAACTATGCAATGGCTTTTGGTAATTTGGCTAGTACGTATTATGAACAAGGTCAACTGGATCTGGCAATCCTACATTATAAGCAAGCCATTTCTTATGATGGAAGGTTTTTGGAGGCTTACAATAATTTGGGTAATGCTCTTAAAGACATTGGCAGAGTAGATGAAGCAATTCAATACTACAATCAATGCCTCACTTTACAGCCTAACCATCCACAAGCGCTTACCAACCTTGGGAATATTTATATGGAATGGAACATGGTGTCTGCTGCTGCTCAATATTATAAGGCCACGTTGACTGTAACAACTGGATTGTCTGCTCCTTTTAATAATCTTGCCATCATCTATAAACAACAGGGGAATTATGCAGATGCAATTTCTTGCTACAATGAGGTTCTCCGCATAGATCCCTTGGCAGCTGATGGGCTTGTCAATAGGGGGAACACATATAAAGAGATTGGTAGAGTCAGTGAAGCAATTCAGGACTACATACACGCAATATCTGTTCGGCCAACTATGGCTGAAGCTCATGCAAATTTGGCGTCAGCTTACAAGGATAGTGGACATGTGGAGGCTGCTATTAAGAGCTATAAACAGGCATTGATTATTCGACCTGATTTTTCAGAGGCAATTTGTAATCTTTTACATACATTACAGTGTGTATGCAGTTGGGAGGACCGCGACAAAATGTTTTCTGAAGTTGAAGGCATAATCAGGAGGCAGATTAATATGTCTCTTCTGCCTAGTGTGCAACCTTTCCATGCAATAGCCTATCCGATTGATCCAATTCTGGCACTTGAAATTAGTCGTAAATATGCTGCACACTGCTCCATAGTGGCATCACGATTTGGACTTTCTCCTTTCAACCATCCTGCTCCAATCCCTATAAAGCGTAATGGTGGACCCCAGAGACTAAGGATTGGATATGTAAGCAGTGATTTTGGTAATCACCCCTTGTCACATCTTATGGGATCTGTGTTTGGGATGCATAATAAGGACAATGTTGAGGTTTTCTGTTATGCCTTGAGTCCAAATGATGGAACGGAATGGAGACAACGCATCCAGTCGGAAGCAGAGCACTTTATGGATGTCTCATCTTTGTCATCAGATATGATTGCCAAAATGATTAATGAAGATAAGATACAGATCTTAATTAACCTTAATGGTTATACAAAGGGTGCAAGAAATGAAATATTTGCCATGCAGCCAGCACCTATTCAGGTTTCATATATGGGTTTCCCTGGTACTACAGGGGCAAATTACATCGATTACTTGGTCACTGATGAGTTTGTTTCACCTTTGCGCTTCTCACATATTTACTCCGAGAAGATTGTTCATCTCCCGCATTGCTACTTTGTGAATGATTACAAGCAGAAAAATCAAGATGTTTTGGAACCAAGCTGTCGGCAGAAGCGATCCAGTTATGGTCTGCCCGAGGATAAATTTATATTTGCATGCTTCAATCAGTTGTACAAAATGGATCCTGAAATCTTTAATACATGGTGCAATATTCTTAAACGTGTACCCAACAGTGCACTTTGGCTCCTCAGATTCCCAGCTCCAGGTGAAATGAGACTTCGTGCATATGCTGTTGCTCAAGGAGTGCAGCCAGACCAAATCATCTTCACAGATGTTGCTATGAAAGGTGAACATATCAAGCGGAGTGCATTGGCAGATTTATTCCTTGACACGCCTCTGTGCAATGCACATACAACAGGCACGGATATTCTATGGGCAGGTCTGCCAATGGTGACCCTTCCCCTTGAAAAGATGGCTACCAGGGTTGCTGGATCACTGTGTCTCGCTACTGGACTGGGAGACGAGATGATTGTTAGCAGCATGAAAGAGTATGAAGAGAAGGCGGTGTCTCTGGCACTGAATCCCCCAAAGCTCTGTGCTCTTACCAGCAAGCTCAAGGCAGCCCGGCTGACTTGTCCTCTGTTTGACACCTTACGCTGGGTGAGGAATCTGGAGAGGAGTTATTTCAAAATGTGGAATCTGCATTGCTCGGGCCAGAAGCCGCATCATTTCAAAGTCACCGAGAACAACTTAGAGTTTCCGTACGATAGATAG

Protein Analysis

981

Amino Acids

110.47

Weight (kDa)

6.79

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_11 PF13414 132 - 172 4.5e-06 TPR repeat
TPR_16 PF13432 136 - 189 2.3e-06 Tetratricopeptide repeat
TPR_8 PF13181 159 - 189 4e-06 Tetratricopeptide repeat
TPR_1 PF00515 159 - 190 1.1e-09 Tetratricopeptide repeat
TPR_2 PF07719 159 - 190 2.3e-08 Tetratricopeptide repeat
TPR_11 PF13414 166 - 204 1.4e-07 TPR repeat
TPR_1 PF00515 226 - 257 7.7e-06 Tetratricopeptide repeat
TPR_11 PF13414 236 - 274 5.8e-11 TPR repeat
TPR_19 PF14559 237 - 292 5.8e-08 Tetratricopeptide repeat
TPR_2 PF07719 260 - 293 5.5e-06 Tetratricopeptide repeat
TPR_1 PF00515 260 - 293 7.4e-10 Tetratricopeptide repeat
TPR_12 PF13424 262 - 321 1.9e-10 Tetratricopeptide repeat
TPR_11 PF13414 278 - 308 9.9e-06 TPR repeat
TPR_11 PF13414 303 - 342 1e-08 TPR repeat
TPR_12 PF13424 328 - 390 4e-07 Tetratricopeptide repeat
TPR_2 PF07719 329 - 361 5.2e-07 Tetratricopeptide repeat
TPR_1 PF00515 329 - 361 2.7e-09 Tetratricopeptide repeat
TPR_8 PF13181 329 - 361 1.6e-08 Tetratricopeptide repeat
TPR_11 PF13414 335 - 373 3.3e-07 TPR repeat
TPR_1 PF00515 399 - 427 6.7e-08 Tetratricopeptide repeat
TPR_2 PF07719 400 - 427 1.6e-06 Tetratricopeptide repeat
TPR_9 PF13371 404 - 467 3.3e-06 Tetratricopeptide repeat
TPR_11 PF13414 405 - 444 1.6e-07 TPR repeat
TPR_11 PF13414 437 - 478 2.7e-07 TPR repeat
Glyco_transf_41 PF13844 511 - 750 1.4e-83 Glycosyl transferase family 41
Glyco_transf_41 PF13844 761 - 946 5.8e-66 Glycosyl transferase family 41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 810, 951, 1155
AbsI CCTCGAGG 1 cut(s) 647
Acc36I ACCTGC 2 cut(s) 778, 2597
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 1907
AccBSI CCGCTC 1 cut(s) 77
AccII CGCG 2 cut(s) 81, 1551
AclWI GGATC 8 cut(s) 939, 1271, 1646, 1849, 2280, 2345, 2358, 2668
AcoI YGGCCR 1 cut(s) 1379
AcsI RAATTY 3 cut(s) 466, 1405, 2312
AcuI CTGAAG 3 cut(s) 758, 1413, 1587
AcyI GRCGYC 2 cut(s) 72, 1412
AfaI GTAC 7 cut(s) 200, 743, 907, 2116, 2342, 2394, 2933
AfeI AGCGCT 1 cut(s) 1092
AfiI CCNNNNNNNGG 5 cut(s) 286, 1104, 1907, 2432, 2826
AflIII ACRYGT 2 cut(s) 1435, 2389
AgsI TTSAA 9 cut(s) 136, 1046, 1574, 1670, 1735, 2332, 2636, 2857, 2902
AjnI CCWGG 4 cut(s) 546, 2110, 2431, 2648
Alw21I GWGCWC 4 cut(s) 859, 1950, 2407, 2770
Alw26I GTCTC 9 cut(s) 133, 1608, 1774, 1915, 1966, 2437, 2675, 2682, 2742
Alw44I GTGCAC 1 cut(s) 2403
AlwI GGATC 8 cut(s) 939, 1271, 1646, 1849, 2280, 2345, 2358, 2668
Ama87I CYCGRG 3 cut(s) 647, 2303, 2878
Aor51HI AGCGCT 1 cut(s) 1092
AoxI GGCC 5 cut(s) 732, 753, 1158, 1379, 2881
ApaI GGGCCC 1 cut(s) 736
ApaLI GTGCAC 1 cut(s) 2403
ApeKI GCWGC 9 cut(s) 1139, 1142, 1286, 1445, 1688, 2074, 2476, 2706, 2789
ApoI RAATTY 3 cut(s) 466, 1405, 2312
ArsI GACNNNNNNTTYG 2 cut(s) 1397, 1429
AseI ATTAAT 2 cut(s) 1596, 1998
Asp700I GAANNNNTTC 3 cut(s) 348, 1041, 1347
AspLEI GCGC 3 cut(s) 74, 1093, 2175
AspS9I GGNCC 5 cut(s) 732, 733, 1546, 1772, 2881
AsuC2I CCSGG 1 cut(s) 2794
AsuHPI GGTGA 8 cut(s) 731, 1811, 2157, 2447, 2528, 2632, 2842, 2899
AsuII TTCGAA 1 cut(s) 352
AvaI CYCGRG 3 cut(s) 647, 2303, 2878
AvaII GGWCC 2 cut(s) 1546, 1772
BaeGI GKGCMC 3 cut(s) 64, 736, 2407
BamHI GGATCC 1 cut(s) 2350
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 736
BarI GAAGNNNNNNTAC 2 cut(s) 1995, 2027
BauI CACGAG 1 cut(s) 153
BbsI GAAGAC 2 cut(s) 174, 523
Bbv12I GWGCWC 4 cut(s) 859, 1950, 2407, 2770
BbvI GCAGC 9 cut(s) 1126, 1129, 1298, 1432, 1675, 2086, 2488, 2718, 2801
BccI CCATC 7 cut(s) 965, 1089, 1217, 1286, 1746, 1901, 2635
BceAI ACGGC 2 cut(s) 94, 740
BciT130I CCWGG 4 cut(s) 548, 2112, 2433, 2650
BciVI GTATCC 1 cut(s) 671
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 1 cut(s) 2794
BcoDI GTCTC 9 cut(s) 133, 1608, 1774, 1915, 1966, 2437, 2675, 2682, 2742
BfaI CTAG 5 cut(s) 57, 227, 495, 903, 1614
BfmI CTRYAG 1 cut(s) 2118
BfoI RGCGCY 2 cut(s) 75, 1094
BfuAI ACCTGC 2 cut(s) 778, 2597
BfuI GTATCC 1 cut(s) 671
BglII AGATCT 1 cut(s) 2016
BlpI GCTNAGC 1 cut(s) 119
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 5 cut(s) 548, 2112, 2433, 2650, 2794
Bme18I GGWCC 2 cut(s) 1546, 1772
BmeT110I CYCGRG 3 cut(s) 647, 2303, 2878
BmgT120I GGNCC 5 cut(s) 732, 733, 1546, 1772, 2881
BmiI GGNNCC 7 cut(s) 73, 108, 734, 1774, 2265, 2352, 2414
BmrFI CCNGG 5 cut(s) 548, 2112, 2433, 2650, 2794
BmrI ACTGGG 1 cut(s) 2693
BmsI GCATC 9 cut(s) 139, 466, 568, 772, 1231, 1718, 1845, 1938, 2902
BmuI ACTGGG 1 cut(s) 2693
BpiI GAAGAC 2 cut(s) 174, 523
BpmI CTGGAG 3 cut(s) 845, 2415, 2861
Bpu1102I GCTNAGC 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 352
BpuEI CTTGAG 3 cut(s) 1915, 2451, 2768
BpuMI CCSGG 1 cut(s) 2794
Bsa29I ATCGAT 1 cut(s) 2136
BsaAI YACGTR 2 cut(s) 659, 909
BsaBI GATNNNNATC 2 cut(s) 786, 2015
BsaHI GRCGYC 2 cut(s) 72, 1412
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 5 cut(s) 1102, 1281, 2110, 2304, 2649
BsaXI ACNNNNNCTCC 6 cut(s) 98, 128, 843, 873, 2397, 2427
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 1104, 1907, 2432, 2826
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 6 cut(s) 933, 1183, 1933, 2289, 2683, 2688
Bse3DI GCAATG 4 cut(s) 888, 2215, 2578, 2872
Bse8I GATNNNNATC 2 cut(s) 786, 2015
BseBI CCWGG 4 cut(s) 548, 2112, 2433, 2650
BseCI ATCGAT 1 cut(s) 2136
BseDI CCNNGG 5 cut(s) 1102, 1281, 2110, 2304, 2649
BseGI GGATG 5 cut(s) 1081, 1738, 1860, 1929, 1963
BseJI GATNNNNATC 2 cut(s) 786, 2015
BseLI CCNNNNNNNGG 5 cut(s) 286, 1104, 1907, 2432, 2826
BseMI GCAATG 4 cut(s) 888, 2215, 2578, 2872
BseMII CTCAG 2 cut(s) 133, 2431
BseNI ACTGG 6 cut(s) 933, 1183, 1933, 2289, 2683, 2688
BseRI GAGGAG 2 cut(s) 2405, 2860
BseSI GKGCMC 3 cut(s) 64, 736, 2407
BseXI GCAGC 9 cut(s) 1126, 1129, 1298, 1432, 1675, 2086, 2488, 2718, 2801
BseYI CCCAGC 2 cut(s) 2425, 2826
BsgI GTGCAG 2 cut(s) 1674, 2495
Bsh1236I CGCG 2 cut(s) 81, 1551
BshFI GGCC 5 cut(s) 734, 755, 1160, 1381, 2883
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 2136
BsiHKAI GWGCWC 4 cut(s) 859, 1950, 2407, 2770
BsiHKCI CYCGRG 3 cut(s) 647, 2303, 2878
BsiSI CCGG 3 cut(s) 38, 104, 2794
BsiWI CGTACG 1 cut(s) 2931
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 5 cut(s) 286, 1104, 1907, 2432, 2826
BsmAI GTCTC 9 cut(s) 133, 1608, 1774, 1915, 1966, 2437, 2675, 2682, 2742
BsmBI CGTCTC 1 cut(s) 2682
BsmFI GGGAC 1 cut(s) 834
BsnI GGCC 5 cut(s) 734, 755, 1160, 1381, 2883
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 3 cut(s) 647, 2303, 2878
Bsp119I TTCGAA 1 cut(s) 352
Bsp120I GGGCCC 1 cut(s) 732
Bsp1286I GDGCHC 6 cut(s) 64, 736, 859, 1950, 2407, 2770
Bsp1407I TGTACA 2 cut(s) 198, 2340
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp68I TCGCGA 1 cut(s) 81
BspANI GGCC 5 cut(s) 734, 755, 1160, 1381, 2883
BspCNI CTCAG 2 cut(s) 132, 2430
BspDI ATCGAT 1 cut(s) 2136
BspFNI CGCG 2 cut(s) 81, 1551
BspLI GGNNCC 7 cut(s) 73, 108, 734, 1774, 2265, 2352, 2414
BspMI ACCTGC 2 cut(s) 778, 2597
BspPI GGATC 8 cut(s) 939, 1271, 1646, 1849, 2280, 2345, 2358, 2668
BspT104I TTCGAA 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 133
BsrBI CCGCTC 1 cut(s) 77
BsrDI GCAATG 4 cut(s) 888, 2215, 2578, 2872
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 2 cut(s) 198, 2340
BsrI ACTGG 6 cut(s) 933, 1183, 1933, 2289, 2683, 2688
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 5 cut(s) 1102, 1281, 2110, 2304, 2649
BssNI GRCGYC 2 cut(s) 72, 1412
BssSI CACGAG 1 cut(s) 153
BssT1I CCWWGG 2 cut(s) 1102, 1281
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 4 cut(s) 548, 2112, 2433, 2650
Bst4CI ACNGT 7 cut(s) 13, 537, 638, 1171, 1527, 2402, 2667
Bst6I CTCTTC 2 cut(s) 1611, 2718
BstACI GRCGYC 2 cut(s) 72, 1412
BstAUI TGTACA 2 cut(s) 198, 2340
BstBAI YACGTR 2 cut(s) 659, 909
BstBI TTCGAA 1 cut(s) 352
BstC8I GCNNGC 6 cut(s) 39, 79, 957, 2079, 2326, 2780
BstDEI CTNAG 5 cut(s) 119, 490, 1784, 2417, 2920
BstEII GGTNACC 1 cut(s) 2620
BstF5I GGATG 5 cut(s) 1081, 1738, 1860, 1929, 1963
BstFNI CGCG 2 cut(s) 81, 1551
BstH2I RGCGCY 2 cut(s) 75, 1094
BstHHI GCGC 3 cut(s) 74, 1093, 2175
BstMAI GTCTC 9 cut(s) 133, 1608, 1774, 1915, 1966, 2437, 2675, 2682, 2742
BstMWI GCNNNNNNNGC 5 cut(s) 59, 78, 115, 1409, 1618
BstNI CCWGG 4 cut(s) 548, 2112, 2433, 2650
BstNSI RCATGY 3 cut(s) 508, 1439, 2328
BstPI GGTNACC 1 cut(s) 2620
BstSCI CCNGG 5 cut(s) 546, 2110, 2431, 2648, 2792
BstSFI CTRYAG 1 cut(s) 2118
BstSLI GKGCMC 3 cut(s) 64, 736, 2407
BstSNI TACGTA 2 cut(s) 659, 909
BstUI CGCG 2 cut(s) 81, 1551
BstV1I GCAGC 9 cut(s) 1126, 1129, 1298, 1432, 1675, 2086, 2488, 2718, 2801
BstV2I GAAGAC 2 cut(s) 174, 523
BstX2I RGATCY 5 cut(s) 931, 1276, 1841, 2016, 2350
BstYI RGATCY 5 cut(s) 931, 1276, 1841, 2016, 2350
Bsu15I ATCGAT 1 cut(s) 2136
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 5 cut(s) 734, 755, 1160, 1381, 2883
BsuTUI ATCGAT 1 cut(s) 2136
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 5 cut(s) 1081, 1738, 1860, 1929, 1963
BtsI GCAGTG 2 cut(s) 1693, 1810
BtsIMutI CAGTG 9 cut(s) 542, 1345, 1532, 1693, 1810, 2148, 2407, 2663, 2744
BtuMI TCGCGA 1 cut(s) 81
BveI ACCTGC 2 cut(s) 778, 2597
Cac8I GCNNGC 6 cut(s) 39, 79, 957, 2079, 2326, 2780
CfoI GCGC 3 cut(s) 74, 1093, 2175
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 5 cut(s) 732, 733, 1546, 1772, 2881
ClaI ATCGAT 1 cut(s) 2136
CseI GACGC 2 cut(s) 179, 1401
Csp6I GTAC 7 cut(s) 199, 742, 906, 2115, 2341, 2393, 2932
CspCI CAANNNNNGTGG 2 cut(s) 1411, 1446
CviQI GTAC 7 cut(s) 199, 742, 906, 2115, 2341, 2393, 2932
DdeI CTNAG 5 cut(s) 119, 490, 1784, 2417, 2920
DinI GGCGCC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 1379
Eam1104I CTCTTC 2 cut(s) 1611, 2718
EarI CTCTTC 2 cut(s) 1611, 2718
Eco105I TACGTA 2 cut(s) 659, 909
Eco130I CCWWGG 2 cut(s) 1102, 1281
Eco24I GRGCYC 1 cut(s) 736
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 2 cut(s) 1546, 1772
Eco47III AGCGCT 1 cut(s) 1092
Eco57I CTGAAG 3 cut(s) 758, 1413, 1587
Eco88I CYCGRG 3 cut(s) 647, 2303, 2878
Eco91I GGTNACC 1 cut(s) 2620
EcoO109I RGGNCCY 2 cut(s) 732, 733
EcoO65I GGTNACC 1 cut(s) 2620
EcoRII CCWGG 4 cut(s) 546, 2110, 2431, 2648
EcoT14I CCWWGG 2 cut(s) 1102, 1281
EcoT22I ATGCAT 2 cut(s) 481, 1860
EcoT38I GRGCYC 1 cut(s) 736
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 1102, 1281
Esp3I CGTCTC 1 cut(s) 2682
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 1 cut(s) 834
FauI CCCGC 1 cut(s) 2220
FauNDI CATATG 2 cut(s) 177, 2455
FbaI TGATCA 1 cut(s) 6
FokI GGATG 5 cut(s) 1068, 1725, 1867, 1916, 1970
FriOI GRGCYC 1 cut(s) 736
FspBI CTAG 5 cut(s) 57, 227, 495, 903, 1614
GlaI GCGC 3 cut(s) 73, 1092, 2174
GsaI CCCAGC 2 cut(s) 2429, 2830
GsuI CTGGAG 3 cut(s) 845, 2415, 2861
HaeII RGCGCY 2 cut(s) 75, 1094
HaeIII GGCC 5 cut(s) 734, 755, 1160, 1381, 2883
HapII CCGG 3 cut(s) 38, 104, 2794
HgaI GACGC 2 cut(s) 179, 1401
HhaI GCGC 3 cut(s) 74, 1093, 2175
Hin1I GRCGYC 2 cut(s) 72, 1412
Hin6I GCGC 3 cut(s) 72, 1091, 2173
HinP1I GCGC 3 cut(s) 72, 1091, 2173
HincII GTYRAC 2 cut(s) 926, 1167
HindII GTYRAC 2 cut(s) 926, 1167
HpaII CCGG 3 cut(s) 38, 104, 2794
HphI GGTGA 8 cut(s) 731, 1811, 2157, 2447, 2528, 2632, 2842, 2899
Hpy166II GTNNAC 7 cut(s) 926, 1167, 1433, 1772, 2393, 2405, 2519
Hpy188I TCNGA 9 cut(s) 50, 465, 1495, 1567, 1647, 1939, 1978, 2194, 2420
Hpy8I GTNNAC 7 cut(s) 926, 1167, 1433, 1772, 2393, 2405, 2519
Hpy99I CGWCG 2 cut(s) 115, 118
HpyAV CCTTC 8 cut(s) 290, 359, 397, 601, 969, 1568, 2636, 2722
HpyCH4III ACNGT 7 cut(s) 13, 537, 638, 1171, 1527, 2402, 2667
HpyCH4IV ACGT 5 cut(s) 658, 853, 908, 1163, 2389
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 78, 115, 1409, 1618
HpyF3I CTNAG 5 cut(s) 119, 490, 1784, 2417, 2920
HpySE526I ACGT 5 cut(s) 658, 853, 908, 1163, 2389
Hsp92I GRCGYC 2 cut(s) 72, 1412
HspAI GCGC 3 cut(s) 72, 1091, 2173
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 6
LmnI GCTCC 7 cut(s) 50, 106, 1195, 1703, 1750, 2418, 2434
Lsp1109I GCAGC 9 cut(s) 1126, 1129, 1298, 1432, 1675, 2086, 2488, 2718, 2801
LweI GCATC 9 cut(s) 139, 466, 568, 772, 1231, 1718, 1845, 1938, 2902
MaeI CTAG 5 cut(s) 57, 227, 495, 903, 1614
MaeII ACGT 5 cut(s) 658, 853, 908, 1163, 2389
MaeIII GTNAC 5 cut(s) 1171, 1827, 2146, 2620, 2905
MbiI CCGCTC 1 cut(s) 77
MflI RGATCY 5 cut(s) 931, 1276, 1841, 2016, 2350
MhlI GDGCHC 6 cut(s) 64, 736, 859, 1950, 2407, 2770
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 722, 1344, 1906
MmeI TCCRAC 3 cut(s) 28, 688, 1917
Mph1103I ATGCAT 2 cut(s) 481, 1860
MreI CGCCGGCG 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 37
MroXI GAANNNNTTC 3 cut(s) 348, 1041, 1347
MslI CAYNNNNRTG 2 cut(s) 147, 1525
MspA1I CMGCKG 1 cut(s) 1289
MspI CCGG 3 cut(s) 38, 104, 2794
MspR9I CCNGG 5 cut(s) 548, 2112, 2433, 2650, 2794
MvaI CCWGG 4 cut(s) 548, 2112, 2433, 2650
MvnI CGCG 2 cut(s) 81, 1551
MwoI GCNNNNNNNGC 5 cut(s) 59, 78, 115, 1409, 1618
NaeI GCCGGC 1 cut(s) 39
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 2794
NdeI CATATG 2 cut(s) 177, 2455
NgoMIV GCCGGC 1 cut(s) 37
NlaIV GGNNCC 7 cut(s) 73, 108, 734, 1774, 2265, 2352, 2414
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 4 cut(s) 1827, 2146, 2620, 2905
NruI TCGCGA 1 cut(s) 81
NsiI ATGCAT 2 cut(s) 481, 1860
NspI RCATGY 3 cut(s) 508, 1439, 2328
NspV TTCGAA 1 cut(s) 352
PacI TTAATTAA 1 cut(s) 2025
PaeI GCATGC 1 cut(s) 2328
PaeR7I CTCGAG 1 cut(s) 647
PciI ACATGT 1 cut(s) 1435
PcsI WCGNNNNNNNCGW 1 cut(s) 2281
PdiI GCCGGC 1 cut(s) 39
PdmI GAANNNNTTC 3 cut(s) 348, 1041, 1347
PfeI GAWTC 7 cut(s) 86, 262, 598, 2421, 2749, 2836, 2866
Pfl23II CGTACG 1 cut(s) 2931
PflMI CCANNNNNTGG 1 cut(s) 1907
PleI GAGTC 3 cut(s) 721, 1343, 1905
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 721, 1343, 1905
Ppu21I YACGTR 2 cut(s) 659, 909
PscI ACATGT 1 cut(s) 1435
PshBI ATTAAT 2 cut(s) 1596, 1998
PsiI TTATAA 3 cut(s) 810, 951, 1155
Psp6I CCWGG 4 cut(s) 546, 2110, 2431, 2648
PspEI GGTNACC 1 cut(s) 2620
PspFI CCCAGC 2 cut(s) 2425, 2826
PspGI CCWGG 4 cut(s) 546, 2110, 2431, 2648
PspLI CGTACG 1 cut(s) 2931
PspN4I GGNNCC 7 cut(s) 73, 108, 734, 1774, 2265, 2352, 2414
PspOMI GGGCCC 1 cut(s) 732
PspPI GGNCC 5 cut(s) 732, 733, 1546, 1772, 2881
PspXI VCTCGAGB 1 cut(s) 647
PsuI RGATCY 5 cut(s) 931, 1276, 1841, 2016, 2350
PvuII CAGCTG 1 cut(s) 1289
RruI TCGCGA 1 cut(s) 81
RsaI GTAC 7 cut(s) 200, 743, 907, 2116, 2342, 2394, 2933
RsaNI GTAC 7 cut(s) 199, 742, 906, 2115, 2341, 2393, 2932
RseI CAYNNNNRTG 2 cut(s) 147, 1525
Sau96I GGNCC 5 cut(s) 732, 733, 1546, 1772, 2881
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 3 cut(s) 722, 1344, 1906
ScrFI CCNGG 5 cut(s) 548, 2112, 2433, 2650, 2794
SduI GDGCHC 6 cut(s) 64, 736, 859, 1950, 2407, 2770
SfaNI GCATC 9 cut(s) 139, 466, 568, 772, 1231, 1718, 1845, 1938, 2902
SfcI CTRYAG 1 cut(s) 2118
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 647
SfuI TTCGAA 1 cut(s) 352
SgrAI CRCCGGYG 1 cut(s) 37
SinI GGWCC 2 cut(s) 1546, 1772
SlaI CTCGAG 1 cut(s) 647
SmiMI CAYNNNNRTG 2 cut(s) 147, 1525
SmlI CTYRAG 4 cut(s) 647, 1894, 2466, 2783
SmoI CTYRAG 4 cut(s) 647, 1894, 2466, 2783
SnaBI TACGTA 2 cut(s) 659, 909
SphI GCATGC 1 cut(s) 2328
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 4 cut(s) 1111, 1151, 2064, 2380
SspMI CTAG 5 cut(s) 57, 227, 495, 903, 1614
StyD4I CCNGG 5 cut(s) 546, 2110, 2431, 2648, 2792
StyI CCWWGG 2 cut(s) 1102, 1281
TaaI ACNGT 7 cut(s) 13, 537, 638, 1171, 1527, 2402, 2667
TaiI ACGT 5 cut(s) 661, 856, 911, 1166, 2392
TaqI TCGA 4 cut(s) 352, 648, 1480, 2136
TatI WGTACW 3 cut(s) 198, 741, 2340
TauI GCSGC 5 cut(s) 37, 77, 364, 533, 2893
TfiI GAWTC 7 cut(s) 86, 262, 598, 2421, 2749, 2836, 2866
TscAI CASTG 9 cut(s) 542, 1345, 1532, 1700, 1810, 2155, 2407, 2670, 2751
TseFI GTSAC 4 cut(s) 1827, 2146, 2620, 2905
TseI GCWGC 9 cut(s) 1139, 1142, 1286, 1445, 1688, 2074, 2476, 2706, 2789
Tsp45I GTSAC 4 cut(s) 1827, 2146, 2620, 2905
TspGWI ACGGA 3 cut(s) 1928, 2606, 2919
TspRI CASTG 9 cut(s) 542, 1345, 1532, 1700, 1810, 2155, 2407, 2670, 2751
Van91I CCANNNNNTGG 1 cut(s) 1907
VneI GTGCAC 1 cut(s) 2403
VpaK11BI GGWCC 2 cut(s) 1546, 1772
VspI ATTAAT 2 cut(s) 1596, 1998
XapI RAATTY 3 cut(s) 466, 1405, 2312
XbaI TCTAGA 1 cut(s) 226
XceI RCATGY 3 cut(s) 508, 1439, 2328
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XhoI CTCGAG 1 cut(s) 647
XmnI GAANNNNTTC 3 cut(s) 348, 1041, 1347
XspI CTAG 5 cut(s) 57, 227, 495, 903, 1614
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 2 cut(s) 481, 1860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.