Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005510

Physical Location & Seq

Genomic Coordinates Reverse (-)

32209334 .. 32212728

Gene Structure 3395 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1247400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGGAGCGTCATCTTCGACCTGTGTGCAGCTCAACGGCCATCTTATGCCCCTCGAAGTCTCTAATTACTAAACTGCCACTGTTACTGCAAAAGCCCACTTCCTCTCCTTGCCGTTTCTCCTCCTACACTTACCCTTCCTCTACACAGCTTTCTACCTCTCATCCGCTTCGTCCTGCAGTTTTAGTGGCGAAGCCGGAGAGCCAAGCAAGCGGCGGGGTCAACACCGACGCAAGAGAATGGGCAATGCAAGATTTTTATTTCTTAAGAAAGGATGTAGAAACTGCCGCAATACGCGTTAAGGAGATAAGAAACTCTGCTTGTCTGCACAAGTTAGAGCAAGAAGTTGCTGATTTGGAGTTGAAAGCAGCTGATACCGCCTTTTGGGATGATCGAGCTCAGGCTCAAGAAAACCTTTCGGCCCTGACTGATGTCAAAGACAGGATAAAATTGCTGAATGAGTTCAAATCACAGGTTGAAGATGTAGAAACAATAGTCAAGCTAACTGAAGAGATGGACTCCATAGACCATGGACTTCTTGAAGAGGCTGCCAGTATCATCAAAGAATTGAACAAGGGAATGGATAGGTTTGAGTTAACTCAACTTCTTTCGGGGCCTTATGACAAGGAAGGTGCTGTCATCTTTATTACAGCTGGTGCTGGAGGTACTGATGCACAGGACTGGGCTGAAATGTTACTCAGGATGTATGTGAGGTGGGCAGAAAAGCAGAGATACAAGACACGAGTAGTTGAGAAATCACAGGGAGAGGAAGCTGGAATTAAGTCAGCGACAATTGAAGTTGAAGGTCGTTATGCTTATGGGTATTTGTCGGGGGAGAAAGGAACACACCGCATTGTAAGGCAGTCCCCTTTTAATGCCAAAGGTCTTCGCCAGACAAGTTTCTCTGGCGTTGATGTCATGCCTCTTCTACCTGAGGAGTCTTTGAAAGTTGAATTACCTGAAGAGGACCTGGACATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGCCAGAATGTGAACAAAATTGAAAGTGCTGTCCGGATTACTCACATCCCGACTGGTGTTACTGTTCGCTGCACAGAGGAGAGGTCCCAGCTTGCAAACAAGATCAAGGCTCTAGCCAGGTTGAAAGCCAAGCTGTTAGTGATTGCCGAGGAGCAAAGGGCGTCGGAAATTAAGCAAATACGAGGAGATGTGGTGAAGGCAGAATGGGGCCAACAAATCAGAAACTACGTGTTCCATCCCTACAAACTCGTCAAGGACGTGAGGACGGGACATGAGACGTCGGATATCACTTCGGTAATGGACGGTGATTTGGAGCCCTACATAAAAGCTTACCTCAAATACAAATACAGTATGAAGGTGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006412 GO:0006415 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0019538 GO:0022411 GO:0032984 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCRF RF-1

Protein Analysis

456

Amino Acids

51.02

Weight (kDa)

6.75

Isoelectric Point (pI)

41.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCRF	PF03462	111 - 303	5.2e-51	PCRF domain
RF-1	PF00472	314 - 422	4.7e-35	RF-1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36170 AT5G36170 AT5G36170 AT5G36170
fragaria_vesca	FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190
malus_domestica	MD04G1247400.v1.1 MD12G1263000.v1.1
prunus_persica	Prupe.6G363800_v2.0.a1 Prupe.6G363800_v2.0.a1
pyrus_communis	pycom04g21810
rosa_chinensis	RchiOBHm_Chr3g0447561
rosa_laevigata	RLG00000025989
rosa_multiflora	Rmu_co8330713.1_g000001 Rmu_co8444065.1_g000001 Rmu_sc0031754.1_g000001 Rmu_sc0037294.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425770
rosa_rugosa	Rorug02G0602900
rosa_samantha	Rh3AG003200 Rh3BG002700 Rh3CG003000 Rh3DG003000
rosa_wichuraiana	Rw3G000210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1283
AccII	CGCG	1 cut(s)	294
AccIII	TCCGGA	1 cut(s)	1035
AciI	CCGC	7 cut(s)	164, 210, 213, 285, 375, 847, 993
AcoI	YGGCCR	1 cut(s)	36
AcuI	CTGAAG	2 cut(s)	525, 978
AcyI	GRCGYC	2 cut(s)	1163, 1280
AfaI	GTAC	1 cut(s)	664
AfiI	CCNNNNNNNGG	1 cut(s)	381
AflII	CTTAAG	1 cut(s)	262
AflIII	ACRYGT	2 cut(s)	292, 1230
AjiI	CACGTC	1 cut(s)	1261
AjnI	CCWGG	2 cut(s)	966, 1118
Alw21I	GWGCWC	1 cut(s)	397
Alw26I	GTCTC	2 cut(s)	63, 1271
Aor13HI	TCCGGA	1 cut(s)	1035
AoxI	GGCC	5 cut(s)	36, 417, 611, 1003, 1210
ApeKI	GCWGC	4 cut(s)	27, 365, 545, 1071
Asp700I	GAANNNNTTC	1 cut(s)	458
AspS9I	GGNCC	5 cut(s)	418, 611, 964, 1086, 1210
AsuHPI	GGTGA	2 cut(s)	1207, 1319
AvaII	GGWCC	2 cut(s)	964, 1086
AxyI	CCTNAGG	1 cut(s)	930
BanII	GRGCYC	2 cut(s)	397, 1320
BauI	CACGAG	1 cut(s)	738
BbsI	GAAGAC	1 cut(s)	875
Bbv12I	GWGCWC	1 cut(s)	397
BbvI	GCAGC	4 cut(s)	39, 377, 532, 1058
BccI	CCATC	3 cut(s)	47, 505, 1245
BceAI	ACGGC	2 cut(s)	51, 96
BcgI	CGANNNNNNTGC	2 cut(s)	6, 40
BciT130I	CCWGG	2 cut(s)	968, 1120
BcoDI	GTCTC	2 cut(s)	63, 1271
BfaI	CTAG	1 cut(s)	1115
BfmI	CTRYAG	1 cut(s)	174
BfrI	CTTAAG	1 cut(s)	262
BisI	GCNGC	6 cut(s)	28, 211, 285, 366, 546, 1072
BlsI	GCNGC	6 cut(s)	29, 212, 286, 367, 547, 1073
Bme1390I	CCNGG	2 cut(s)	968, 1120
Bme18I	GGWCC	2 cut(s)	964, 1086
BmgBI	CACGTC	1 cut(s)	1261
BmgT120I	GGNCC	5 cut(s)	418, 611, 964, 1086, 1210
BmiI	GGNNCC	4 cut(s)	612, 1088, 1211, 1317
BmrFI	CCNGG	2 cut(s)	968, 1120
BmrI	ACTGGG	1 cut(s)	688
BmsI	GCATC	1 cut(s)	658
BmuI	ACTGGG	1 cut(s)	688
BoxI	GACNNNNGTC	1 cut(s)	428
BpiI	GAAGAC	1 cut(s)	875
BplI	GAGNNNNNCTC	2 cut(s)	500, 532
BpmI	CTGGAG	1 cut(s)	678
Bpu10I	CCTNAGC	1 cut(s)	396
BpuEI	CTTGAG	1 cut(s)	387
BsaAI	YACGTR	1 cut(s)	1231
BsaHI	GRCGYC	2 cut(s)	1163, 1280
BsaJI	CCNNGG	2 cut(s)	526, 1149
BsaWI	WCCGGW	1 cut(s)	1035
BsaXI	ACNNNNNCTCC	4 cut(s)	88, 118, 1179, 1209
Bsc4I	CCNNNNNNNGG	1 cut(s)	381
Bse1I	ACTGG	3 cut(s)	549, 683, 1060
Bse21I	CCTNAGG	1 cut(s)	930
Bse3DI	GCAATG	1 cut(s)	249
BseAI	TCCGGA	1 cut(s)	1035
BseBI	CCWGG	2 cut(s)	968, 1120
BseDI	CCNNGG	2 cut(s)	526, 1149
BseGI	GGATG	6 cut(s)	160, 277, 391, 705, 1047, 1237
BseLI	CCNNNNNNNGG	1 cut(s)	381
BseMI	GCAATG	1 cut(s)	249
BseMII	CTCAG	3 cut(s)	410, 709, 921
BseNI	ACTGG	3 cut(s)	549, 683, 1060
BseRI	GAGGAG	5 cut(s)	109, 947, 1094, 1166, 1200
BseXI	GCAGC	4 cut(s)	39, 377, 532, 1058
BseYI	CCCAGC	1 cut(s)	1089
BsgI	GTGCAG	3 cut(s)	46, 308, 1057
Bsh1236I	CGCG	1 cut(s)	294
BshFI	GGCC	5 cut(s)	38, 419, 613, 1005, 1212
BsiHKAI	GWGCWC	1 cut(s)	397
BsiSI	CCGG	2 cut(s)	194, 1036
BslFI	GGGAC	3 cut(s)	847, 1072, 1284
BslI	CCNNNNNNNGG	1 cut(s)	381
BsmAI	GTCTC	2 cut(s)	63, 1271
BsmBI	CGTCTC	1 cut(s)	1271
BsmFI	GGGAC	3 cut(s)	847, 1072, 1284
BsnI	GGCC	5 cut(s)	38, 419, 613, 1005, 1212
Bsp1286I	GDGCHC	2 cut(s)	397, 1320
Bsp13I	TCCGGA	1 cut(s)	1035
Bsp143I	GATC	2 cut(s)	388, 1104
Bsp19I	CCATGG	1 cut(s)	526
BspACI	CCGC	7 cut(s)	164, 210, 213, 285, 375, 847, 993
BspANI	GGCC	5 cut(s)	38, 419, 613, 1005, 1212
BspCNI	CTCAG	3 cut(s)	409, 708, 922
BspEI	TCCGGA	1 cut(s)	1035
BspFNI	CGCG	1 cut(s)	294
BspLI	GGNNCC	4 cut(s)	612, 1088, 1211, 1317
BspMAI	CTGCAG	1 cut(s)	178
BspTI	CTTAAG	1 cut(s)	262
BsrDI	GCAATG	1 cut(s)	249
BsrI	ACTGG	3 cut(s)	549, 683, 1060
BssECI	CCNNGG	2 cut(s)	526, 1149
BssMI	GATC	2 cut(s)	388, 1104
BssNI	GRCGYC	2 cut(s)	1163, 1280
BssSI	CACGAG	1 cut(s)	738
BssT1I	CCWWGG	1 cut(s)	526
Bst2BI	CACGAG	1 cut(s)	738
Bst2UI	CCWGG	2 cut(s)	968, 1120
Bst4CI	ACNGT	4 cut(s)	81, 1066, 1307, 1352
Bst6I	CTCTTC	4 cut(s)	501, 534, 927, 954
BstACI	GRCGYC	2 cut(s)	1163, 1280
BstAFI	CTTAAG	1 cut(s)	262
BstBAI	YACGTR	1 cut(s)	1231
BstC8I	GCNNGC	3 cut(s)	208, 991, 1095
BstDEI	CTNAG	3 cut(s)	396, 695, 930
BstDSI	CCRYGG	1 cut(s)	526
BstF5I	GGATG	6 cut(s)	160, 277, 391, 705, 1047, 1237
BstFNI	CGCG	1 cut(s)	294
BstKTI	GATC	2 cut(s)	391, 1107
BstMAI	GTCTC	2 cut(s)	63, 1271
BstMBI	GATC	2 cut(s)	388, 1104
BstMWI	GCNNNNNNNGC	2 cut(s)	207, 374
BstNI	CCWGG	2 cut(s)	968, 1120
BstPAI	GACNNNNGTC	1 cut(s)	428
BstSCI	CCNGG	2 cut(s)	966, 1118
BstSFI	CTRYAG	1 cut(s)	174
BstUI	CGCG	1 cut(s)	294
BstV1I	GCAGC	4 cut(s)	39, 377, 532, 1058
BstV2I	GAAGAC	1 cut(s)	875
Bsu36I	CCTNAGG	1 cut(s)	930
BsuRI	GGCC	5 cut(s)	38, 419, 613, 1005, 1212
BtgI	CCRYGG	1 cut(s)	526
BtrI	CACGTC	1 cut(s)	1261
BtsCI	GGATG	6 cut(s)	160, 277, 391, 705, 1047, 1237
BtsIMutI	CAGTG	1 cut(s)	77
Cac8I	GCNNGC	3 cut(s)	208, 991, 1095
Cfr13I	GGNCC	5 cut(s)	418, 611, 964, 1086, 1210
CseI	GACGC	2 cut(s)	236, 1152
Csp6I	GTAC	1 cut(s)	663
CviAII	CATG	3 cut(s)	527, 916, 1274
CviQI	GTAC	1 cut(s)	663
DdeI	CTNAG	3 cut(s)	396, 695, 930
DpnI	GATC	2 cut(s)	390, 1106
DpnII	GATC	2 cut(s)	388, 1104
EaeI	YGGCCR	1 cut(s)	36
Eam1104I	CTCTTC	4 cut(s)	501, 534, 927, 954
EarI	CTCTTC	4 cut(s)	501, 534, 927, 954
EciI	GGCGGA	1 cut(s)	1008
Ecl136II	GAGCTC	1 cut(s)	395
Eco130I	CCWWGG	1 cut(s)	526
Eco24I	GRGCYC	2 cut(s)	397, 1320
Eco32I	GATATC	1 cut(s)	1288
Eco47I	GGWCC	2 cut(s)	964, 1086
Eco53kI	GAGCTC	1 cut(s)	395
Eco57I	CTGAAG	2 cut(s)	525, 978
Eco81I	CCTNAGG	1 cut(s)	930
EcoICRI	GAGCTC	1 cut(s)	395
EcoO109I	RGGNCCY	3 cut(s)	611, 964, 1086
EcoRII	CCWGG	2 cut(s)	966, 1118
EcoRV	GATATC	1 cut(s)	1288
EcoT14I	CCWWGG	1 cut(s)	526
EcoT38I	GRGCYC	2 cut(s)	397, 1320
ErhI	CCWWGG	1 cut(s)	526
Esp3I	CGTCTC	1 cut(s)	1271
FaeI	CATG	3 cut(s)	530, 919, 1277
FalI	AAGNNNNNCTT	2 cut(s)	396, 428
FaqI	GGGAC	3 cut(s)	847, 1072, 1284
FatI	CATG	3 cut(s)	526, 915, 1273
FauI	CCCGC	1 cut(s)	206
Fnu4HI	GCNGC	6 cut(s)	28, 211, 285, 366, 546, 1072
FokI	GGATG	6 cut(s)	147, 284, 398, 712, 1034, 1224
FriOI	GRGCYC	2 cut(s)	397, 1320
Fsp4HI	GCNGC	6 cut(s)	28, 211, 285, 366, 546, 1072
FspBI	CTAG	1 cut(s)	1115
GluI	GCNGC	6 cut(s)	28, 211, 285, 366, 546, 1072
GsaI	CCCAGC	1 cut(s)	1093
GsuI	CTGGAG	1 cut(s)	678
HaeIII	GGCC	5 cut(s)	38, 419, 613, 1005, 1212
HapII	CCGG	2 cut(s)	194, 1036
HgaI	GACGC	2 cut(s)	236, 1152
Hin1I	GRCGYC	2 cut(s)	1163, 1280
Hin1II	CATG	3 cut(s)	530, 919, 1277
HincII	GTYRAC	2 cut(s)	220, 594
HindII	GTYRAC	2 cut(s)	220, 594
HindIII	AAGCTT	1 cut(s)	1329
HinfI	GANTC	2 cut(s)	515, 935
HpaI	GTTAAC	1 cut(s)	594
HpaII	CCGG	2 cut(s)	194, 1036
HphI	GGTGA	2 cut(s)	1207, 1319
Hpy166II	GTNNAC	3 cut(s)	220, 594, 1015
Hpy188I	TCNGA	3 cut(s)	1168, 1223, 1285
Hpy188III	TCNNGA	5 cut(s)	404, 536, 697, 1036, 1051
Hpy8I	GTNNAC	3 cut(s)	220, 594, 1015
Hpy99I	CGWCG	3 cut(s)	230, 1168, 1285
HpyAV	CCTTC	5 cut(s)	144, 620, 794, 1192, 1351
HpyCH4III	ACNGT	4 cut(s)	81, 1066, 1307, 1352
HpyCH4IV	ACGT	3 cut(s)	1230, 1260, 1280
HpyCH4V	TGCA	8 cut(s)	27, 88, 176, 247, 325, 671, 1074, 1097
HpyF10VI	GCNNNNNNNGC	2 cut(s)	207, 374
HpyF3I	CTNAG	3 cut(s)	396, 695, 930
HpySE526I	ACGT	3 cut(s)	1230, 1260, 1280
Hsp92I	GRCGYC	2 cut(s)	1163, 1280
Hsp92II	CATG	3 cut(s)	530, 919, 1277
Kpn2I	TCCGGA	1 cut(s)	1035
KspAI	GTTAAC	1 cut(s)	594
Kzo9I	GATC	2 cut(s)	388, 1104
LmnI	GCTCC	3 cut(s)	4, 1153, 1315
Lsp1109I	GCAGC	4 cut(s)	39, 377, 532, 1058
LweI	GCATC	1 cut(s)	658
MaeI	CTAG	1 cut(s)	1115
MaeII	ACGT	3 cut(s)	1230, 1260, 1280
MaeIII	GTNAC	3 cut(s)	81, 690, 1060
MalI	GATC	2 cut(s)	390, 1106
MboI	GATC	2 cut(s)	388, 1104
MboII	GAAGA	7 cut(s)	5, 488, 518, 551, 875, 914, 971
MfeI	CAATTG	1 cut(s)	789
MhlI	GDGCHC	2 cut(s)	397, 1320
MluCI	AATT	8 cut(s)	63, 446, 563, 774, 789, 950, 1020, 1170
MluI	ACGCGT	1 cut(s)	292
MlyI	GAGTC	2 cut(s)	509, 944
MmeI	TCCRAC	2 cut(s)	1146, 1263
MroI	TCCGGA	1 cut(s)	1035
MroXI	GAANNNNTTC	1 cut(s)	458
MseI	TTAA	6 cut(s)	263, 297, 593, 777, 870, 1173
MspA1I	CMGCKG	2 cut(s)	368, 650
MspCI	CTTAAG	1 cut(s)	262
MspI	CCGG	2 cut(s)	194, 1036
MspR9I	CCNGG	2 cut(s)	968, 1120
MunI	CAATTG	1 cut(s)	789
MvaI	CCWGG	2 cut(s)	968, 1120
MvnI	CGCG	1 cut(s)	294
MwoI	GCNNNNNNNGC	2 cut(s)	207, 374
NcoI	CCATGG	1 cut(s)	526
NdeII	GATC	2 cut(s)	388, 1104
NlaIII	CATG	3 cut(s)	530, 919, 1277
NlaIV	GGNNCC	4 cut(s)	612, 1088, 1211, 1317
NmeAIII	GCCGAG	1 cut(s)	1174
PcsI	WCGNNNNNNNCGW	1 cut(s)	1257
PdmI	GAANNNNTTC	1 cut(s)	458
PkrI	GCNGC	6 cut(s)	29, 212, 286, 367, 547, 1073
PleI	GAGTC	2 cut(s)	509, 943
PpsI	GAGTC	2 cut(s)	509, 943
Ppu21I	YACGTR	1 cut(s)	1231
PpuMI	RGGWCCY	2 cut(s)	964, 1086
PshAI	GACNNNNGTC	1 cut(s)	428
Psp124BI	GAGCTC	1 cut(s)	397
Psp5II	RGGWCCY	2 cut(s)	964, 1086
Psp6I	CCWGG	2 cut(s)	966, 1118
PspFI	CCCAGC	1 cut(s)	1089
PspGI	CCWGG	2 cut(s)	966, 1118
PspN4I	GGNNCC	4 cut(s)	612, 1088, 1211, 1317
PspPI	GGNCC	5 cut(s)	418, 611, 964, 1086, 1210
PspPPI	RGGWCCY	2 cut(s)	964, 1086
PstI	CTGCAG	1 cut(s)	178
PvuII	CAGCTG	2 cut(s)	368, 650
RsaI	GTAC	1 cut(s)	664
RsaNI	GTAC	1 cut(s)	663
SacI	GAGCTC	1 cut(s)	397
SaqAI	TTAA	6 cut(s)	263, 297, 593, 777, 870, 1173
SatI	GCNGC	6 cut(s)	28, 211, 285, 366, 546, 1072
Sau3AI	GATC	2 cut(s)	388, 1104
Sau96I	GGNCC	5 cut(s)	418, 611, 964, 1086, 1210
SchI	GAGTC	2 cut(s)	509, 944
ScrFI	CCNGG	2 cut(s)	968, 1120
SduI	GDGCHC	2 cut(s)	397, 1320
SfaNI	GCATC	1 cut(s)	658
SfcI	CTRYAG	1 cut(s)	174
SinI	GGWCC	2 cut(s)	964, 1086
SmlI	CTYRAG	2 cut(s)	262, 402
SmoI	CTYRAG	2 cut(s)	262, 402
Sse9I	AATT	8 cut(s)	63, 446, 563, 774, 789, 950, 1020, 1170
SsiI	CCGC	7 cut(s)	164, 210, 213, 285, 375, 847, 993
SspMI	CTAG	1 cut(s)	1115
SstI	GAGCTC	1 cut(s)	397
StyD4I	CCNGG	2 cut(s)	966, 1118
StyI	CCWWGG	1 cut(s)	526
TaaI	ACNGT	4 cut(s)	81, 1066, 1307, 1352
TaiI	ACGT	3 cut(s)	1233, 1263, 1283
TaqI	TCGA	3 cut(s)	16, 53, 391
TasI	AATT	8 cut(s)	63, 446, 563, 774, 789, 950, 1020, 1170
TauI	GCSGC	2 cut(s)	213, 287
Tru1I	TTAA	6 cut(s)	263, 297, 593, 777, 870, 1173
Tru9I	TTAA	6 cut(s)	263, 297, 593, 777, 870, 1173
TscAI	CASTG	1 cut(s)	84
TseI	GCWGC	4 cut(s)	27, 365, 545, 1071
TspRI	CASTG	1 cut(s)	84
Vha464I	CTTAAG	1 cut(s)	262
VpaK11BI	GGWCC	2 cut(s)	964, 1086
XmnI	GAANNNNTTC	1 cut(s)	458
XspI	CTAG	1 cut(s)	1115
ZraI	GACGTC	1 cut(s)	1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.