Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005510

Physical Location & Seq

Genomic Coordinates Forward (+)

46766966 .. 46771490

Gene Structure 4525 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00425770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGCGGTTCAGAGGCAGGGGGGACTTGGACCTCATTCACTTACTCGAAGCGAAAGCCACATCCATGGAGGTTCATCTAAAACCTCTATGCAGCTCAACAACTATCTTATCCCCCAAAGCTTCAAATACTAGACTACCACAGTTACCACGCAAGTCCACTTCCTCTTCTGCTTCTCGTATATCCTACAATTTACCCTTCTCCACTACGCTTTCCACACCTTCTAGATTCTCTCCATCTGTTTTATTTGCGACACCGGAGAGTCAAGTAAGCGCGGAAGTCAACACCAACGAAAGAGAATGGGCAATGCAAGATTTCTATACATTGAGGAGGGAGGTAGAAATTGCGGCAGGACGTGTTGAAGAGATAAGAGCTTCCGCTTGTCTGGAACAGTTGGAAAAAGAAGTTGCCGAGTTGGAATCCAAAGCAGCTGATAGCTCCTTTTGGGATGATCGAGCTAAGGCTCAAGAGAACCTTTTGGCCCTCACTGATGTCAAAGACAGAATAAAATCGCTTACTCACTTCAAAACACAGGTTGAAGATGCAGAAACAATAGTTAAGCTAACTGAAGAGATGGACTCCACAGATAATGCACTTCTTGAAGAGGCTGCCAGCATCATCAAGGAATTGAACAAGGGAATGGATCGGTTTGAGTTAACTCAACTTCTTTCAGGGCCTTATGACAAGGAAGGTGCTGTTATTTCTGTTACAGCCGGTGCTGGAGGCACTGACGCACAGGATTGGGCTGAAATGTTACTCAGGATGTATATGAGGTGGGGAGAAAAGCAGAGATACAAAACTCGAGTGGTTGAGAGATCCCCAGGAGAGGAAGCTGGAATTAAGTCGGCGACACTCGAAGTTGAAGGTCGTTATGCTTATGGTTATTTGTCTGGGGAGAAAGGCACACACCGCATTGTACGACAGTCTCCTTTTAATGCCAAAGGTCTTCGCCAGACAAGCTTCTCTGGTGTGGAAGTGATGCCTCTCCTACCTGAGGAATCACTGAAAGTTGAAATACCTGAAGAAGACCTGGAGATAACCACTTCAAGGGCAGGTGGGAAAGGAGGTCAGAATGTGAATAAAATTGAAAGTGCTGTCCGGATTACTCACATCCCCACTGGTGTTACTGTTCGCTGCACAGAAGAGAGATCCCAGCTTGCAAACAGAATAAAGGCTCTAAGCAGGTTGAAAGCGAAGCTGTTGGTGATAGCTGAGGAGCAACGAGCATCGGAAATTAAGCAAATACGAGGAGATGCAGTGAAGGCAGAGTGGGGGCAACAAATAAGGAACTATGTGTTCCATCCGTACAAACTCGTCAAGGATGTAAGGACAGGATATGAGACATCTGATATCACTTCTGTGATGGACGGTGATTTGGAGCCTTACATCAAAGCCTACCTCAAACATAAATACAGTATGAAAATAAGCGCATCAAGTAAAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006412 GO:0006415 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0019538 GO:0022411 GO:0032984 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCRF RF-1

Protein Analysis

480

Amino Acids

53.64

Weight (kDa)

6.47

Isoelectric Point (pI)

42.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCRF	PF03462	131 - 323	2.2e-51	PCRF domain
RF-1	PF00472	334 - 442	3e-36	RF-1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36170 AT5G36170 AT5G36170 AT5G36170
fragaria_vesca	FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190 FvH4_6g00190
malus_domestica	MD04G1247400.v1.1 MD12G1263000.v1.1
prunus_persica	Prupe.6G363800_v2.0.a1 Prupe.6G363800_v2.0.a1
pyrus_communis	pycom04g21810
rosa_chinensis	RchiOBHm_Chr3g0447561
rosa_laevigata	RLG00000025989
rosa_multiflora	Rmu_co8330713.1_g000001 Rmu_co8444065.1_g000001 Rmu_sc0031754.1_g000001 Rmu_sc0037294.1_g000002
rosa_roxburghii	Rroxscaffold_6G00425770
rosa_rugosa	Rorug02G0602900
rosa_samantha	Rh3AG003200 Rh3BG002700 Rh3CG003000 Rh3DG003000
rosa_wichuraiana	Rw3G000210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1040
Acc36I	ACCTGC	2 cut(s)	1040, 1170
AccII	CGCG	1 cut(s)	272
AccIII	TCCGGA	1 cut(s)	1095
AciI	CCGC	5 cut(s)	4, 272, 344, 375, 907
AclWI	GGATC	3 cut(s)	648, 807, 1140
AcuI	CTGAAG	2 cut(s)	585, 1038
AfaI	GTAC	2 cut(s)	915, 1304
AfiI	CCNNNNNNNGG	3 cut(s)	823, 991, 1044
AflIII	ACRYGT	1 cut(s)	352
AjiI	CACGTC	1 cut(s)	353
AjnI	CCWGG	2 cut(s)	817, 1026
AleI	CACNNNNGTG	1 cut(s)	1355
Alw26I	GTCTC	2 cut(s)	927, 1331
AlwI	GGATC	3 cut(s)	648, 807, 1140
Ama87I	CYCGRG	1 cut(s)	798
Aor13HI	TCCGGA	1 cut(s)	1095
AoxI	GGCC	2 cut(s)	477, 671
ApeKI	GCWGC	4 cut(s)	90, 425, 605, 1131
AspLEI	GCGC	2 cut(s)	272, 1427
AspS9I	GGNCC	3 cut(s)	28, 478, 671
AsuHPI	GGTGA	2 cut(s)	1213, 1379
AvaI	CYCGRG	1 cut(s)	798
AvaII	GGWCC	1 cut(s)	28
AxyI	CCTNAGG	1 cut(s)	990
BbsI	GAAGAC	2 cut(s)	935, 1029
BbvCI	CCTCAGC	1 cut(s)	1209
BbvI	GCAGC	4 cut(s)	102, 437, 592, 1118
BccI	CCATC	4 cut(s)	241, 565, 1305, 1354
BciT130I	CCWGG	2 cut(s)	819, 1028
BcoDI	GTCTC	2 cut(s)	927, 1331
BfaI	CTAG	2 cut(s)	129, 222
BfuAI	ACCTGC	2 cut(s)	1040, 1170
BisI	GCNGC	5 cut(s)	91, 345, 426, 606, 1132
BlsI	GCNGC	5 cut(s)	92, 346, 427, 607, 1133
Bme1390I	CCNGG	2 cut(s)	819, 1028
Bme18I	GGWCC	1 cut(s)	28
BmeT110I	CYCGRG	1 cut(s)	798
BmgBI	CACGTC	1 cut(s)	353
BmgT120I	GGNCC	3 cut(s)	28, 478, 671
BmiI	GGNNCC	1 cut(s)	1377
BmrFI	CCNGG	2 cut(s)	819, 1028
BmsI	GCATC	6 cut(s)	529, 621, 966, 1232, 1240, 1436
BpiI	GAAGAC	2 cut(s)	935, 1029
BplI	GAGNNNNNCTC	2 cut(s)	560, 592
BpmI	CTGGAG	2 cut(s)	738, 1049
Bpu10I	CCTNAGC	2 cut(s)	456, 1209
BpuEI	CTTGAG	1 cut(s)	447
BsaJI	CCNNGG	2 cut(s)	63, 817
BsaWI	WCCGGW	2 cut(s)	253, 1095
BsaXI	ACNNNNNCTCC	2 cut(s)	1239, 1269
Bsc4I	CCNNNNNNNGG	3 cut(s)	823, 991, 1044
Bse118I	RCCGGY	1 cut(s)	710
Bse1I	ACTGG	1 cut(s)	1120
Bse21I	CCTNAGG	1 cut(s)	990
Bse3DI	GCAATG	1 cut(s)	309
BseAI	TCCGGA	1 cut(s)	1095
BseBI	CCWGG	2 cut(s)	819, 1028
BseDI	CCNNGG	2 cut(s)	63, 817
BseGI	GGATG	6 cut(s)	59, 451, 765, 1107, 1297, 1324
BseLI	CCNNNNNNNGG	3 cut(s)	823, 991, 1044
BseMI	GCAATG	1 cut(s)	309
BseMII	CTCAG	3 cut(s)	769, 981, 1200
BseNI	ACTGG	1 cut(s)	1120
BseRI	GAGGAG	3 cut(s)	340, 1226, 1260
BseXI	GCAGC	4 cut(s)	102, 437, 592, 1118
BseYI	CCCAGC	1 cut(s)	1149
BsgI	GTGCAG	1 cut(s)	1117
Bsh1236I	CGCG	1 cut(s)	272
BshFI	GGCC	2 cut(s)	479, 673
BsiHKCI	CYCGRG	1 cut(s)	798
BsiSI	CCGG	3 cut(s)	254, 711, 1096
BslFI	GGGAC	1 cut(s)	35
BslI	CCNNNNNNNGG	3 cut(s)	823, 991, 1044
BsmAI	GTCTC	2 cut(s)	927, 1331
BsmFI	GGGAC	1 cut(s)	35
BsnI	GGCC	2 cut(s)	479, 673
BsoBI	CYCGRG	1 cut(s)	798
Bsp13I	TCCGGA	1 cut(s)	1095
Bsp143I	GATC	4 cut(s)	448, 640, 812, 1145
Bsp19I	CCATGG	1 cut(s)	63
BspACI	CCGC	5 cut(s)	4, 272, 344, 375, 907
BspANI	GGCC	2 cut(s)	479, 673
BspCNI	CTCAG	3 cut(s)	768, 982, 1201
BspEI	TCCGGA	1 cut(s)	1095
BspFNI	CGCG	1 cut(s)	272
BspLI	GGNNCC	1 cut(s)	1377
BspMI	ACCTGC	2 cut(s)	1040, 1170
BspPI	GGATC	3 cut(s)	648, 807, 1140
BsrDI	GCAATG	1 cut(s)	309
BsrFI	RCCGGY	1 cut(s)	710
BsrI	ACTGG	1 cut(s)	1120
BssAI	RCCGGY	1 cut(s)	710
BssECI	CCNNGG	2 cut(s)	63, 817
BssMI	GATC	4 cut(s)	448, 640, 812, 1145
BssT1I	CCWWGG	1 cut(s)	63
Bst2UI	CCWGG	2 cut(s)	819, 1028
Bst4CI	ACNGT	6 cut(s)	141, 390, 921, 1126, 1367, 1412
Bst6I	CTCTTC	5 cut(s)	169, 354, 561, 594, 1134
BstC8I	GCNNGC	2 cut(s)	610, 1155
BstDEI	CTNAG	6 cut(s)	456, 755, 990, 1175, 1209, 1440
BstDSI	CCRYGG	1 cut(s)	63
BstENI	CCTNNNNNAGG	1 cut(s)	989
BstF5I	GGATG	6 cut(s)	59, 451, 765, 1107, 1297, 1324
BstFNI	CGCG	1 cut(s)	272
BstHHI	GCGC	2 cut(s)	272, 1427
BstKTI	GATC	4 cut(s)	451, 643, 815, 1148
BstMAI	GTCTC	2 cut(s)	927, 1331
BstMBI	GATC	4 cut(s)	448, 640, 812, 1145
BstMWI	GCNNNNNNNGC	3 cut(s)	906, 954, 1259
BstNI	CCWGG	2 cut(s)	819, 1028
BstSCI	CCNGG	2 cut(s)	817, 1026
BstUI	CGCG	1 cut(s)	272
BstV1I	GCAGC	4 cut(s)	102, 437, 592, 1118
BstV2I	GAAGAC	2 cut(s)	935, 1029
BstX2I	RGATCY	2 cut(s)	812, 1145
BstXI	CCANNNNNNTGG	1 cut(s)	64
BstYI	RGATCY	2 cut(s)	812, 1145
Bsu36I	CCTNAGG	1 cut(s)	990
BsuRI	GGCC	2 cut(s)	479, 673
BtgI	CCRYGG	1 cut(s)	63
BtrI	CACGTC	1 cut(s)	353
BtsCI	GGATG	6 cut(s)	59, 451, 765, 1107, 1297, 1324
BtsI	GCAGTG	1 cut(s)	1260
BtsIMutI	CAGTG	5 cut(s)	483, 723, 998, 1113, 1260
BveI	ACCTGC	2 cut(s)	1040, 1170
Cac8I	GCNNGC	2 cut(s)	610, 1155
CfoI	GCGC	2 cut(s)	272, 1427
Cfr10I	RCCGGY	1 cut(s)	710
Cfr13I	GGNCC	3 cut(s)	28, 478, 671
CseI	GACGC	1 cut(s)	737
Csp6I	GTAC	2 cut(s)	914, 1303
CviAII	CATG	1 cut(s)	64
CviQI	GTAC	2 cut(s)	914, 1303
DdeI	CTNAG	6 cut(s)	456, 755, 990, 1175, 1209, 1440
DpnI	GATC	4 cut(s)	450, 642, 814, 1147
DpnII	GATC	4 cut(s)	448, 640, 812, 1145
Eam1104I	CTCTTC	5 cut(s)	169, 354, 561, 594, 1134
EarI	CTCTTC	5 cut(s)	169, 354, 561, 594, 1134
Eco130I	CCWWGG	1 cut(s)	63
Eco32I	GATATC	1 cut(s)	1348
Eco47I	GGWCC	1 cut(s)	28
Eco57I	CTGAAG	2 cut(s)	585, 1038
Eco81I	CCTNAGG	1 cut(s)	990
Eco88I	CYCGRG	1 cut(s)	798
EcoNI	CCTNNNNNAGG	1 cut(s)	989
EcoO109I	RGGNCCY	1 cut(s)	671
EcoRII	CCWGG	2 cut(s)	817, 1026
EcoRV	GATATC	1 cut(s)	1348
EcoT14I	CCWWGG	1 cut(s)	63
ErhI	CCWWGG	1 cut(s)	63
FaeI	CATG	1 cut(s)	67
FalI	AAGNNNNNCTT	3 cut(s)	456, 488, 1423
FaqI	GGGAC	1 cut(s)	35
FatI	CATG	1 cut(s)	63
Fnu4HI	GCNGC	5 cut(s)	91, 345, 426, 606, 1132
FokI	GGATG	6 cut(s)	46, 458, 772, 1094, 1284, 1331
Fsp4HI	GCNGC	5 cut(s)	91, 345, 426, 606, 1132
FspBI	CTAG	2 cut(s)	129, 222
GlaI	GCGC	2 cut(s)	271, 1426
GluI	GCNGC	5 cut(s)	91, 345, 426, 606, 1132
GsaI	CCCAGC	1 cut(s)	1153
GsuI	CTGGAG	2 cut(s)	738, 1049
HaeIII	GGCC	2 cut(s)	479, 673
HapII	CCGG	3 cut(s)	254, 711, 1096
HgaI	GACGC	1 cut(s)	737
HhaI	GCGC	2 cut(s)	272, 1427
Hin1II	CATG	1 cut(s)	67
Hin6I	GCGC	2 cut(s)	270, 1425
HinP1I	GCGC	2 cut(s)	270, 1425
HincII	GTYRAC	2 cut(s)	280, 654
HindII	GTYRAC	2 cut(s)	280, 654
HindIII	AAGCTT	3 cut(s)	117, 955, 1437
HinfI	GANTC	5 cut(s)	225, 259, 416, 575, 995
HpaI	GTTAAC	1 cut(s)	654
HpaII	CCGG	3 cut(s)	254, 711, 1096
HphI	GGTGA	2 cut(s)	1213, 1379
Hpy166II	GTNNAC	3 cut(s)	156, 280, 654
Hpy188I	TCNGA	4 cut(s)	11, 1068, 1228, 1345
Hpy188III	TCNNGA	6 cut(s)	222, 383, 464, 596, 757, 1096
Hpy8I	GTNNAC	3 cut(s)	156, 280, 654
HpyAV	CCTTC	5 cut(s)	205, 228, 680, 854, 1252
HpyCH4III	ACNGT	6 cut(s)	141, 390, 921, 1126, 1367, 1412
HpyCH4IV	ACGT	1 cut(s)	352
HpyCH4V	TGCA	7 cut(s)	90, 307, 542, 590, 1134, 1157, 1253
HpyF10VI	GCNNNNNNNGC	3 cut(s)	906, 954, 1259
HpyF3I	CTNAG	6 cut(s)	456, 755, 990, 1175, 1209, 1440
HpySE526I	ACGT	1 cut(s)	352
Hsp92II	CATG	1 cut(s)	67
HspAI	GCGC	2 cut(s)	270, 1425
Kpn2I	TCCGGA	1 cut(s)	1095
KspAI	GTTAAC	1 cut(s)	654
Kzo9I	GATC	4 cut(s)	448, 640, 812, 1145
LmnI	GCTCC	3 cut(s)	440, 1213, 1375
Lsp1109I	GCAGC	4 cut(s)	102, 437, 592, 1118
LweI	GCATC	6 cut(s)	529, 621, 966, 1232, 1240, 1436
MaeI	CTAG	2 cut(s)	129, 222
MaeII	ACGT	1 cut(s)	352
MaeIII	GTNAC	4 cut(s)	141, 703, 750, 1120
MalI	GATC	4 cut(s)	450, 642, 814, 1147
MboI	GATC	4 cut(s)	448, 640, 812, 1145
MboII	GAAGA	9 cut(s)	156, 371, 548, 578, 611, 935, 1031, 1034, 1151
MflI	RGATCY	2 cut(s)	812, 1145
MluCI	AATT	6 cut(s)	187, 339, 623, 834, 1080, 1230
MlyI	GAGTC	2 cut(s)	268, 569
MmeI	TCCRAC	2 cut(s)	372, 393
MroI	TCCGGA	1 cut(s)	1095
MseI	TTAA	5 cut(s)	555, 653, 837, 930, 1233
MslI	CAYNNNNRTG	2 cut(s)	62, 1355
MspA1I	CMGCKG	1 cut(s)	428
MspI	CCGG	3 cut(s)	254, 711, 1096
MspR9I	CCNGG	2 cut(s)	819, 1028
MvaI	CCWGG	2 cut(s)	819, 1028
MvnI	CGCG	1 cut(s)	272
MwoI	GCNNNNNNNGC	3 cut(s)	906, 954, 1259
NcoI	CCATGG	1 cut(s)	63
NdeII	GATC	4 cut(s)	448, 640, 812, 1145
NlaIII	CATG	1 cut(s)	67
NlaIV	GGNNCC	1 cut(s)	1377
NmeAIII	GCCGAG	1 cut(s)	433
OliI	CACNNNNGTG	1 cut(s)	1355
PaeR7I	CTCGAG	1 cut(s)	798
PaqCI	CACCTGC	1 cut(s)	1040
PfeI	GAWTC	3 cut(s)	225, 416, 995
PkrI	GCNGC	5 cut(s)	92, 346, 427, 607, 1133
PleI	GAGTC	2 cut(s)	267, 569
PpsI	GAGTC	2 cut(s)	267, 569
Psp6I	CCWGG	2 cut(s)	817, 1026
PspFI	CCCAGC	1 cut(s)	1149
PspGI	CCWGG	2 cut(s)	817, 1026
PspN4I	GGNNCC	1 cut(s)	1377
PspPI	GGNCC	3 cut(s)	28, 478, 671
PspXI	VCTCGAGB	1 cut(s)	798
PsuI	RGATCY	2 cut(s)	812, 1145
PvuII	CAGCTG	1 cut(s)	428
RsaI	GTAC	2 cut(s)	915, 1304
RsaNI	GTAC	2 cut(s)	914, 1303
RseI	CAYNNNNRTG	2 cut(s)	62, 1355
SaqAI	TTAA	5 cut(s)	555, 653, 837, 930, 1233
SatI	GCNGC	5 cut(s)	91, 345, 426, 606, 1132
Sau3AI	GATC	4 cut(s)	448, 640, 812, 1145
Sau96I	GGNCC	3 cut(s)	28, 478, 671
SchI	GAGTC	2 cut(s)	268, 569
ScrFI	CCNGG	2 cut(s)	819, 1028
SfaNI	GCATC	6 cut(s)	529, 621, 966, 1232, 1240, 1436
Sfr274I	CTCGAG	1 cut(s)	798
SinI	GGWCC	1 cut(s)	28
SlaI	CTCGAG	1 cut(s)	798
SmiMI	CAYNNNNRTG	2 cut(s)	62, 1355
SmlI	CTYRAG	2 cut(s)	462, 798
SmoI	CTYRAG	2 cut(s)	462, 798
Sse9I	AATT	6 cut(s)	187, 339, 623, 834, 1080, 1230
SsiI	CCGC	5 cut(s)	4, 272, 344, 375, 907
SspMI	CTAG	2 cut(s)	129, 222
StyD4I	CCNGG	2 cut(s)	817, 1026
StyI	CCWWGG	1 cut(s)	63
TaaI	ACNGT	6 cut(s)	141, 390, 921, 1126, 1367, 1412
TaiI	ACGT	1 cut(s)	355
TaqI	TCGA	4 cut(s)	45, 451, 799, 852
TasI	AATT	6 cut(s)	187, 339, 623, 834, 1080, 1230
TauI	GCSGC	1 cut(s)	347
TfiI	GAWTC	3 cut(s)	225, 416, 995
Tru1I	TTAA	5 cut(s)	555, 653, 837, 930, 1233
Tru9I	TTAA	5 cut(s)	555, 653, 837, 930, 1233
TscAI	CASTG	5 cut(s)	490, 730, 1005, 1120, 1260
TseI	GCWGC	4 cut(s)	90, 425, 605, 1131
TspDTI	ATGAA	2 cut(s)	62, 1430
TspGWI	ACGGA	1 cut(s)	1290
TspRI	CASTG	5 cut(s)	490, 730, 1005, 1120, 1260
VpaK11BI	GGWCC	1 cut(s)	28
XagI	CCTNNNNNAGG	1 cut(s)	989
XbaI	TCTAGA	1 cut(s)	221
XhoI	CTCGAG	1 cut(s)	798
XspI	CTAG	2 cut(s)	129, 222

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.