MD05G1018200.v1.1

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015373
Physical Location & Seq
Reverse (-)
3198536 .. 3199447
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1018200.v1.1.491

Sequence Viewer

Length: 912 bp
ATGTTCACAGATCCTACTGTTAAACAAATGCTACAGTGCCCTCCATCTGGGCAAGGGCAATTCATGATGATGGAGATGAAGAGACCATGGAACAAATCCCATATTGAGGTTGCCCCAAACTGCCCTCGATGTGCCTCTTCCAACACCAAATTTTGCTATTACAACAACTACAGCCTCTCACAGCCAAGGTACTTTTGCAAAGGGTGCAGGAGATATTGGACAAAAGGAGGCTCCTTACGCAACGTCCCAGTTGGTGGTGGCTGCCGCAAGAACCGCCGTGGTAAAGCGACCAGGCTCTCACAGACTGACAGCGCTTCATTAAGTTACTTTCAGCATCACAACTCATCAAGTGGTACTGAAGATGATCCGTCCGGAAATAATCAGCCAGGTGGATCTAATGCATCAGATATTGATTTGGCTGTTGTGTTTGCCAAATTCTTAAACCACAACTCCACTACCCCTGATGATCATTATCATGACCCCAATTTGGTAATTTCAAGCTCAGCATTGAACGATCACGATGGCTCACAAAATTCTTCCAAGGCTGGTGATCTTGTTGAAGCAGTTGATCATTATTTACTTGAAGGACATCAACAATCACAGGAAGAAAATGTTCAAACTTTTATGGGAATTAATCACAATGATGATATGAACATTCATGAATTTGAGTTACAGGGTTTACCAGTAGACGAAGACCAAGTGGTGCAAGATGTGTTTTGGTCCGATGATGCAACGATGTCGTCTTTGGCAAGCAGTACTGCTAGTTTTACATGGCAACCGATGGTGCATTTGCAAGAGTTGGACTGTTCACTACCATCAGACGATGATCAAATGAAGATATCGACCAATTTATGTGGCGATAATTGGAGCACCTTTGATTTCTCAGGGTTTGAAGTTTTCTCACGATCTTAA

Protein Analysis

304

Amino Acids

33.86

Weight (kDa)

5.01

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 39 - 93 4.5e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21340
fragaria_vesca FvH4_2g14390
malus_domestica MD05G1018200.v1.1 MD10G1017800.v1.1
prunus_persica Prupe.8G022200_v2.0.a1
pyrus_communis pycom05g01080
rosa_chinensis RchiOBHm_Chr6g0276721
rosa_laevigata RLG00000013370
rosa_multiflora Rmu_sc0000263.1_g000003
rosa_roxburghii Rroxscaffold_7G00192050
rosa_rugosa Rorug06G0103100
rosa_samantha Rh6AG213500 Rh6BG218400 Rh6CG221200 Rh6DG211100
rosa_wichuraiana Rw6G018670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 687
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 2 cut(s) 265, 274
AclWI GGATC 3 cut(s) 5, 359, 400
AcsI RAATTY 4 cut(s) 149, 434, 532, 662
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 3 cut(s) 191, 355, 757
AfeI AGCGCT 1 cut(s) 313
AfiI CCNNNNNNNGG 4 cut(s) 47, 106, 254, 487
AgsI TTSAA 6 cut(s) 498, 511, 560, 584, 617, 893
AjnI CCWGG 2 cut(s) 290, 385
AluBI AGCT 1 cut(s) 501
AluI AGCT 1 cut(s) 501
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 3 cut(s) 5, 359, 400
Aor13HI TCCGGA 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 313
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 4 cut(s) 149, 434, 532, 662
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 612
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 560
AvaII GGWCC 1 cut(s) 720
BaeGI GKGCMC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 872
BbvI GCAGC 1 cut(s) 248
BccI CCATC 5 cut(s) 52, 64, 515, 775, 823
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 2 cut(s) 292, 387
BclI TGATCA 3 cut(s) 466, 568, 826
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 32, 169
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 2 cut(s) 262, 265
BlpI GCTNAGC 1 cut(s) 502
BlsI GCNGC 2 cut(s) 263, 266
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 2 cut(s) 292, 387
Bme18I GGWCC 1 cut(s) 720
BmgT120I GGNCC 1 cut(s) 720
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 292, 387
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 343, 410, 718
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 86, 185, 277, 540
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 106, 254, 487
Bse1I ACTGG 2 cut(s) 248, 683
Bse8I GATNNNNATC 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 2 cut(s) 292, 387
BseDI CCNNGG 4 cut(s) 86, 185, 277, 540
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 4 cut(s) 47, 106, 254, 487
BseMII CTCAG 2 cut(s) 516, 897
BseNI ACTGG 2 cut(s) 248, 683
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 4 cut(s) 47, 106, 254, 487
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 41, 872
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
Bsp1720I GCTNAGC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 2 cut(s) 265, 274
BspCNI CTCAG 2 cut(s) 515, 896
BspEI TCCGGA 1 cut(s) 371
BspHI TCATGA 3 cut(s) 63, 475, 658
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 3 cut(s) 5, 359, 400
BspTNI GGTCTC 1 cut(s) 76
BsrI ACTGG 2 cut(s) 248, 683
BssECI CCNNGG 4 cut(s) 86, 185, 277, 540
BssMI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
BssT1I CCWWGG 3 cut(s) 86, 185, 540
Bst2UI CCWGG 2 cut(s) 292, 387
Bst4CI ACNGT 3 cut(s) 19, 36, 806
Bst6I CTCTTC 2 cut(s) 74, 142
BstAPI GCANNNNNTGC 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 2 cut(s) 502, 883
BstDSI CCRYGG 2 cut(s) 86, 277
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 9 cut(s) 13, 367, 395, 469, 517, 553, 571, 829, 908
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
BstMWI GCNNNNNNNGC 3 cut(s) 204, 237, 273
BstNI CCWGG 2 cut(s) 292, 387
BstSCI CCNGG 2 cut(s) 290, 385
BstSFI CTRYAG 2 cut(s) 32, 169
BstSLI GKGCMC 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 2 cut(s) 10, 392
BstYI RGATCY 2 cut(s) 10, 392
BtgI CCRYGG 2 cut(s) 86, 277
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 1 cut(s) 751
CciI TCATGA 3 cut(s) 63, 475, 658
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 720
Csp6I GTAC 3 cut(s) 190, 354, 756
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 5 cut(s) 64, 87, 476, 659, 771
CviQI GTAC 3 cut(s) 190, 354, 756
DdeI CTNAG 2 cut(s) 502, 883
DpnI GATC 9 cut(s) 12, 366, 394, 468, 516, 552, 570, 828, 907
DpnII GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
Eam1104I CTCTTC 2 cut(s) 74, 142
EarI CTCTTC 2 cut(s) 74, 142
Eco130I CCWWGG 3 cut(s) 86, 185, 540
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 720
Eco47III AGCGCT 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 378
EcoRII CCWGG 2 cut(s) 290, 385
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 3 cut(s) 86, 185, 540
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 3 cut(s) 86, 185, 540
FaeI CATG 5 cut(s) 67, 90, 479, 662, 774
FaiI YATR 9 cut(s) 65, 88, 102, 477, 626, 650, 660, 772, 853
FaqI GGGAC 1 cut(s) 230
FatI CATG 5 cut(s) 63, 86, 475, 658, 770
FbaI TGATCA 3 cut(s) 466, 568, 826
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 2 cut(s) 262, 265
Fsp4HI GCNGC 2 cut(s) 262, 265
FspBI CTAG 1 cut(s) 762
GlaI GCGC 1 cut(s) 313
GluI GCNGC 2 cut(s) 262, 265
HaeII RGCGCY 1 cut(s) 315
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 314
Hin1II CATG 5 cut(s) 67, 90, 479, 662, 774
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HpaII CCGG 1 cut(s) 372
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 4 cut(s) 6, 680, 688, 809
Hpy188I TCNGA 3 cut(s) 406, 724, 820
Hpy188III TCNNGA 6 cut(s) 64, 372, 476, 518, 659, 903
Hpy8I GTNNAC 4 cut(s) 6, 680, 688, 809
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 3 cut(s) 19, 36, 806
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 7 cut(s) 198, 207, 401, 706, 731, 787, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 237, 273
HpyF3I CTNAG 2 cut(s) 502, 883
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 5 cut(s) 67, 90, 479, 662, 774
HspAI GCGC 1 cut(s) 312
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 3 cut(s) 466, 568, 826
Kzo9I GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
LmnI GCTCC 2 cut(s) 236, 867
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 3 cut(s) 343, 410, 718
MaeI CTAG 1 cut(s) 762
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 323, 669
MalI GATC 9 cut(s) 12, 366, 394, 468, 516, 552, 570, 828, 907
MboI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
MboII GAAGA 7 cut(s) 91, 129, 371, 528, 617, 704, 847
MflI RGATCY 2 cut(s) 10, 392
MhlI GDGCHC 2 cut(s) 41, 872
MmeI TCCRAC 2 cut(s) 165, 780
MnlI CCTC 6 cut(s) 51, 100, 135, 145, 185, 221
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 5 cut(s) 21, 320, 440, 633, 910
MslI CAYNNNNRTG 3 cut(s) 68, 474, 642
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 292, 387
MvaI CCWGG 2 cut(s) 292, 387
MwoI GCNNNNNNNGC 3 cut(s) 204, 237, 273
NcoI CCATGG 1 cut(s) 86
NdeII GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
NlaIII CATG 5 cut(s) 67, 90, 479, 662, 774
NlaIV GGNNCC 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 403
PagI TCATGA 3 cut(s) 63, 475, 658
PdmI GAANNNNTTC 1 cut(s) 612
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 2 cut(s) 263, 266
PshBI ATTAAT 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 290, 385
PspGI CCWGG 2 cut(s) 290, 385
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 720
PsuI RGATCY 2 cut(s) 10, 392
RsaI GTAC 3 cut(s) 191, 355, 757
RsaNI GTAC 3 cut(s) 190, 354, 756
RseI CAYNNNNRTG 3 cut(s) 68, 474, 642
SaqAI TTAA 5 cut(s) 21, 320, 440, 633, 910
SatI GCNGC 2 cut(s) 262, 265
Sau3AI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
Sau96I GGNCC 1 cut(s) 720
ScaI AGTACT 1 cut(s) 757
ScrFI CCNGG 2 cut(s) 292, 387
SduI GDGCHC 2 cut(s) 41, 872
SetI ASST 6 cut(s) 111, 191, 246, 391, 503, 875
SfaNI GCATC 3 cut(s) 343, 410, 718
SfcI CTRYAG 2 cut(s) 32, 169
SinI GGWCC 1 cut(s) 720
SmiMI CAYNNNNRTG 3 cut(s) 68, 474, 642
SsiI CCGC 2 cut(s) 265, 274
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 2 cut(s) 290, 385
StyI CCWWGG 3 cut(s) 86, 185, 540
TaaI ACNGT 3 cut(s) 19, 36, 806
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 127, 842
TatI WGTACW 1 cut(s) 755
TauI GCSGC 1 cut(s) 267
Tru1I TTAA 5 cut(s) 21, 320, 440, 633, 910
Tru9I TTAA 5 cut(s) 21, 320, 440, 633, 910
TscAI CASTG 1 cut(s) 41
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 7 cut(s) 52, 92, 306, 647, 665, 675, 848
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 720
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 4 cut(s) 149, 434, 532, 662
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 1 cut(s) 762
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.