RLG00000013370

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015373
Physical Location & Seq
Reverse (-)
30450660 .. 30452213
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013370

Sequence Viewer

Length: 903 bp
ATGTTCACAGCTCCAGTTCTCGAACAGATGCTGCACTCTCAATCCGGACAATTGGTCATGAACCAGAAGCCGTCGTGGAAGCCCCACGTTGAGATCGCTCCCAATTGCCCCCGCTGCGCGTCTTCCAACACCAAGTTCTGCTACTACAACAACTACAGCTTCTCGCAGCCGCGCTACTTCTGCAAAGGCTGCCGGAGATACTGGACCAAAGGCGGGTCCCTGAGAAACGTGCCCGTCGGCGGCGGTTGTAGAAAGAACCGTCGCGGCAGGTCGGTCAGGCTCTCGCAGGCTGACCGGGTTTCGTTGAGTTACTATCGTCACAACAGCTCGAGTAGTAGCGGTGGTGATCTATCCGAGCAGCAGTCCATGGAAGGTGGAGCCAACGGATCGGATATCGACTTGGCTGCTGTGTTTGCTAGGTTCTTGAACCAAGACTCTAGCAACGAACATGATCCCAATTTGGTTGGATCAGAATCGATCCCCGATGATGTTCATGAGGCAAACGCATTATCTGATCACTCCCAAATTTCTTCGAACCCCCCCGCGGAAGAGCTTCTTGAATCAGTGGAGCTACTTGAAGGACTCGTACTTCCTGATCAGGATCATCAACAATTACACGAAGAGGATAATATTCAGAATATTATGGGAAATCAACACCATCATGATTTGAGTATTCATGAATTTGGGTTGCAGGGTTTGCTAGGCGATGAGGATGTGTTTTGGGCTGATACAACTACACGTTCGACAAGCAGTACTACTAGTTTCACATGGCAAGAGTTGGATAATTCATTCCCATCTAATGATGATGATCATATGAAGATATCGACCACCACGACGAATCTATGTAGCGATAACTGGAGCTCCTTTGATTTTTCAGGCTTGGAAGTTTTCTCAGGATCATAA

Protein Analysis

301

Amino Acids

33.41

Weight (kDa)

4.93

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 34 - 88 1e-31 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21340
fragaria_vesca FvH4_2g14390
malus_domestica MD05G1018200.v1.1 MD10G1017800.v1.1
prunus_persica Prupe.8G022200_v2.0.a1
pyrus_communis pycom05g01080
rosa_chinensis RchiOBHm_Chr6g0276721
rosa_laevigata RLG00000013370
rosa_multiflora Rmu_sc0000263.1_g000003
rosa_roxburghii Rroxscaffold_7G00192050
rosa_rugosa Rorug06G0103100
rosa_samantha Rh6AG213500 Rh6BG218400 Rh6CG221200 Rh6DG211100
rosa_wichuraiana Rw6G018670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 258
AccII CGCG 4 cut(s) 119, 172, 264, 545
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 9 cut(s) 112, 170, 213, 240, 243, 264, 339, 543, 545
AclWI GGATC 5 cut(s) 394, 446, 472, 475, 609
AcsI RAATTY 2 cut(s) 525, 680
AfaI GTAC 2 cut(s) 588, 754
AfiI CCNNNNNNNGG 3 cut(s) 213, 239, 544
AflIII ACRYGT 1 cut(s) 737
AgsI TTSAA 3 cut(s) 427, 560, 578
AhdI GACNNNNNGTC 1 cut(s) 53
AhlI ACTAGT 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 6 cut(s) 11, 159, 327, 553, 571, 861
AluI AGCT 6 cut(s) 11, 159, 327, 553, 571, 861
Alw21I GWGCWC 1 cut(s) 863
AlwI GGATC 5 cut(s) 394, 446, 472, 475, 609
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 328
Aor13HI TCCGGA 1 cut(s) 44
ApeKI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
ApoI RAATTY 2 cut(s) 525, 680
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 2 cut(s) 119, 174
AspS9I GGNCC 2 cut(s) 204, 216
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 356
AsuII TTCGAA 1 cut(s) 533
AvaI CYCGRG 1 cut(s) 328
AvaII GGWCC 2 cut(s) 204, 216
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 863
BarI GAAGNNNNNNTAC 2 cut(s) 570, 602
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 863
BbvI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BccI CCATC 2 cut(s) 666, 802
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BclI TGATCA 3 cut(s) 514, 595, 808
BcnI CCSGG 1 cut(s) 296
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 4 cut(s) 417, 438, 701, 759
BfmI CTRYAG 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 258
BisI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
BlsI GCNGC 9 cut(s) 33, 116, 168, 171, 191, 242, 266, 360, 406
BmcAI AGTACT 1 cut(s) 754
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 204, 216
BmeRI GACNNNNNGTC 1 cut(s) 53
BmeT110I CYCGRG 1 cut(s) 328
BmgT120I GGNCC 2 cut(s) 204, 216
BmiI GGNNCC 3 cut(s) 217, 218, 379
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 877
Bpu14I TTCGAA 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 476
BsaBI GATNNNNATC 3 cut(s) 472, 600, 807
BsaJI CCNNGG 2 cut(s) 366, 543
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 239, 544
Bse1I ACTGG 3 cut(s) 14, 206, 860
Bse8I GATNNNNATC 3 cut(s) 472, 600, 807
BseAI TCCGGA 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 2 cut(s) 366, 543
BseGI GGATG 1 cut(s) 718
BseJI GATNNNNATC 3 cut(s) 472, 600, 807
BseLI CCNNNNNNNGG 3 cut(s) 213, 239, 544
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 3 cut(s) 14, 206, 860
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BsgI GTGCAG 1 cut(s) 17
Bsh1236I CGCG 4 cut(s) 119, 172, 264, 545
BshVI ATCGAT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 863
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 3 cut(s) 45, 193, 295
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 213, 239, 544
BsmFI GGGAC 1 cut(s) 202
BsoBI CYCGRG 1 cut(s) 328
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 234, 863
Bsp13I TCCGGA 1 cut(s) 44
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 9 cut(s) 112, 170, 213, 240, 243, 264, 339, 543, 545
BspCNI CTCAG 1 cut(s) 213
BspDI ATCGAT 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 44
BspFNI CGCG 4 cut(s) 119, 172, 264, 545
BspHI TCATGA 4 cut(s) 57, 493, 661, 676
BspLI GGNNCC 3 cut(s) 217, 218, 379
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 5 cut(s) 394, 446, 472, 475, 609
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 533
BsrI ACTGG 3 cut(s) 14, 206, 860
BssECI CCNNGG 2 cut(s) 366, 543
BssT1I CCWWGG 1 cut(s) 366
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 2 cut(s) 543, 615
BstAPI GCANNNNNTGC 2 cut(s) 189, 697
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 221, 892
BstDSI CCRYGG 2 cut(s) 366, 543
BstF5I GGATG 1 cut(s) 718
BstFNI CGCG 4 cut(s) 119, 172, 264, 545
BstHHI GCGC 2 cut(s) 119, 174
BstMWI GCNNNNNNNGC 5 cut(s) 114, 180, 189, 413, 697
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 4 cut(s) 119, 172, 264, 545
BstV1I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BstV2I GAAGAC 1 cut(s) 114
Bsu15I ATCGAT 1 cut(s) 476
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 2 cut(s) 366, 543
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 1 cut(s) 570
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 4 cut(s) 57, 493, 661, 676
CfoI GCGC 2 cut(s) 119, 174
Cfr13I GGNCC 2 cut(s) 204, 216
Cfr42I CCGCGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 108
Csp6I GTAC 2 cut(s) 587, 753
CviAII CATG 7 cut(s) 58, 367, 449, 494, 662, 677, 768
CviQI GTAC 2 cut(s) 587, 753
DdeI CTNAG 2 cut(s) 221, 892
DriI GACNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 2 cut(s) 543, 615
Eam1105I GACNNNNNGTC 1 cut(s) 53
EarI CTCTTC 2 cut(s) 543, 615
Ecl136II GAGCTC 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 863
Eco32I GATATC 2 cut(s) 394, 822
Eco47I GGWCC 2 cut(s) 204, 216
Eco53kI GAGCTC 1 cut(s) 861
Eco88I CYCGRG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 216
EcoRV GATATC 2 cut(s) 394, 822
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 863
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 7 cut(s) 61, 370, 452, 497, 665, 680, 771
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 1 cut(s) 202
FatI CATG 7 cut(s) 57, 366, 448, 493, 661, 676, 767
FauI CCCGC 3 cut(s) 119, 206, 550
FauNDI CATATG 1 cut(s) 813
FbaI TGATCA 3 cut(s) 514, 595, 808
Fnu4HI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
FokI GGATG 1 cut(s) 725
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
FspBI CTAG 4 cut(s) 417, 438, 701, 759
GlaI GCGC 2 cut(s) 118, 173
GluI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
GsuI CTGGAG 1 cut(s) 877
HapII CCGG 3 cut(s) 45, 193, 295
HgaI GACGC 1 cut(s) 108
HhaI GCGC 2 cut(s) 119, 174
Hin1II CATG 7 cut(s) 61, 370, 452, 497, 665, 680, 771
Hin6I GCGC 2 cut(s) 117, 172
HinP1I GCGC 2 cut(s) 117, 172
HinfI GANTC 5 cut(s) 434, 473, 560, 582, 838
HpaII CCGG 3 cut(s) 45, 193, 295
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 5 cut(s) 355, 391, 472, 514, 636
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 4 cut(s) 76, 239, 264, 838
HpyAV CCTTC 2 cut(s) 365, 572
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 3 cut(s) 87, 228, 739
HpyCH4V TGCA 3 cut(s) 34, 183, 691
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 180, 189, 413, 697
HpyF3I CTNAG 2 cut(s) 221, 892
HpySE526I ACGT 3 cut(s) 87, 228, 739
Hsp92II CATG 7 cut(s) 61, 370, 452, 497, 665, 680, 771
HspAI GCGC 2 cut(s) 117, 172
KflI GGGWCCC 1 cut(s) 216
Kpn2I TCCGGA 1 cut(s) 44
Ksp22I TGATCA 3 cut(s) 514, 595, 808
KspI CCGCGG 1 cut(s) 546
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 6 cut(s) 16, 103, 377, 568, 858, 866
Lsp1109I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
LweI GCATC 1 cut(s) 18
MaeI CTAG 4 cut(s) 417, 438, 701, 759
MaeII ACGT 3 cut(s) 87, 228, 739
MaeIII GTNAC 2 cut(s) 308, 317
MboII GAAGA 5 cut(s) 114, 522, 560, 632, 829
MfeI CAATTG 2 cut(s) 50, 103
MhlI GDGCHC 2 cut(s) 234, 863
MluCI AATT 7 cut(s) 50, 103, 457, 525, 611, 680, 784
MlyI GAGTC 2 cut(s) 428, 576
MmeI TCCRAC 3 cut(s) 150, 445, 759
MnlI CCTC 3 cut(s) 490, 616, 703
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 552
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 2 cut(s) 114, 545
MspI CCGG 3 cut(s) 45, 193, 295
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 2 cut(s) 50, 103
MvnI CGCG 4 cut(s) 119, 172, 264, 545
MwoI GCNNNNNNNGC 5 cut(s) 114, 180, 189, 413, 697
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 813
NlaIII CATG 7 cut(s) 61, 370, 452, 497, 665, 680, 771
NlaIV GGNNCC 3 cut(s) 217, 218, 379
NmuCI GTSAC 1 cut(s) 317
NspV TTCGAA 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 328
PagI TCATGA 4 cut(s) 57, 493, 661, 676
PciSI GCTCTTC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 3 cut(s) 473, 560, 838
PkrI GCNGC 9 cut(s) 33, 116, 168, 171, 191, 242, 266, 360, 406
PleI GAGTC 2 cut(s) 428, 576
PpsI GAGTC 2 cut(s) 428, 576
PpuMI RGGWCCY 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 863
Psp5II RGGWCCY 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 217, 218, 379
PspPI GGNCC 2 cut(s) 204, 216
PspPPI RGGWCCY 1 cut(s) 216
PspXI VCTCGAGB 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 2 cut(s) 588, 754
RsaNI GTAC 2 cut(s) 587, 753
RseI CAYNNNNRTG 1 cut(s) 660
SacI GAGCTC 1 cut(s) 863
SacII CCGCGG 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 543
SatI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
Sau96I GGNCC 2 cut(s) 204, 216
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 2 cut(s) 428, 576
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 234, 863
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 328
Sfr303I CCGCGG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 533
SgrBI CCGCGG 1 cut(s) 546
SinI GGWCC 2 cut(s) 204, 216
SlaI CTCGAG 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 660
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 7 cut(s) 50, 103, 457, 525, 611, 680, 784
SsiI CCGC 9 cut(s) 112, 170, 213, 240, 243, 264, 339, 543, 545
SspI AATATT 2 cut(s) 631, 640
SspMI CTAG 4 cut(s) 417, 438, 701, 759
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 3 cut(s) 90, 231, 742
TaqI TCGA 7 cut(s) 21, 329, 396, 476, 533, 743, 824
TaqII GACCGA 1 cut(s) 262
TasI AATT 7 cut(s) 50, 103, 457, 525, 611, 680, 784
TatI WGTACW 1 cut(s) 752
TauI GCSGC 3 cut(s) 172, 243, 267
TfiI GAWTC 3 cut(s) 473, 560, 838
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 6 cut(s) 74, 482, 665, 693, 777, 830
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 204, 216
XapI RAATTY 2 cut(s) 525, 680
XhoI CTCGAG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 4 cut(s) 417, 438, 701, 759
ZrmI AGTACT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.