pycom05g01080

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015373
Physical Location & Seq
Reverse (-)
1296880 .. 1297791
912 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01080.1

Sequence Viewer

Length: 912 bp
ATGTTCACAGATCCTACTGTTAAACAAATGCTACAGTGCCATCCATCTGGGCAAGGGCAATTCATGATGATGGAGATGAAGAGACCATGGAACAAATCCCATATTGAGGTTGCCCCAAACTGCCCTCGATGTGCCTCTTCCAACACCAAATTTTGCTATTACAACAACTACAGCCTCTCACAGCCAAGGTACTTTTGCAAAGGGTGCAGGAGATATTGGACAAAAGGAGGCTCCTTACGCAACGTCCCAGTTGGTGGCGGCTGCCGCAAGAACCGCCGCGGTAAAGCGGCCAGACTCTCACAGCCTGACCGCGCTTCATTAAGTTACTTTCAGCATCACAACTCATCAAGTGGTACTGAAGATGATCCGTCCGGAAATAATCAGCCAGGTGGATCTAATGCATCAGATATTGATTTGGCTGTTGTGTTTGCCAAATTCTTAAACCACAACTCCACTACCCCTGATGATCATTATCATGACCCCAATTTGGTAATTTCAAGCTCAGCATTGAACGATCACGATGGCTCACAAAATTCTTCCAAGGCTGATGATCTTGTTGAAGCAGTTGATCATTATTTACTTGAAGGACATCAACAATCACAGGAAGAAAATGTTCAAACGTTTATGGGAATTAATCACAATGATGATATGAACATTCATGAATTTGAGTTACAGGGTTTACTAGGAGACGAAGACCAAGTGGTGCAAGATGTGTTTTGGTCCCATGATGCAACGACGTCGTCTTTGCCAAGCAGTACTGCTAGTTTTACATGGCAACCGATGGTGCATTTGCAAGAGTTGGACTGTTCACTACCATCAGACGATGATCAAATGAAGATATCGACCAATTTATGTGGCGACAATTGGAGCTCCATTGATTTTTCAGGGTTTGAAGTTTTCTCACGATCTTAA

Protein Analysis

304

Amino Acids

33.93

Weight (kDa)

5.25

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 39 - 93 4.5e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21340
fragaria_vesca FvH4_2g14390
malus_domestica MD05G1018200.v1.1 MD10G1017800.v1.1
prunus_persica Prupe.8G022200_v2.0.a1
pyrus_communis pycom05g01080
rosa_chinensis RchiOBHm_Chr6g0276721
rosa_laevigata RLG00000013370
rosa_multiflora Rmu_sc0000263.1_g000003
rosa_roxburghii Rroxscaffold_7G00192050
rosa_rugosa Rorug06G0103100
rosa_samantha Rh6AG213500 Rh6BG218400 Rh6CG221200 Rh6DG211100
rosa_wichuraiana Rw6G018670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 740
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 2 cut(s) 279, 312
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 7 cut(s) 258, 265, 274, 277, 279, 287, 310
AclI AACGTT 1 cut(s) 620
AclWI GGATC 3 cut(s) 5, 359, 400
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 4 cut(s) 149, 434, 532, 662
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 737
AfaI GTAC 3 cut(s) 191, 355, 757
AfiI CCNNNNNNNGG 3 cut(s) 106, 254, 487
AgsI TTSAA 6 cut(s) 498, 511, 560, 584, 617, 893
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 2 cut(s) 501, 870
AluI AGCT 2 cut(s) 501, 870
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 2 cut(s) 76, 681
AlwI GGATC 3 cut(s) 5, 359, 400
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 4 cut(s) 149, 434, 532, 662
ArsI GACNNNNNNTTYG 2 cut(s) 727, 759
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 612
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 720
AvaII GGWCC 1 cut(s) 720
BanII GRGCYC 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 872
BbvI GCAGC 1 cut(s) 248
BccI CCATC 6 cut(s) 48, 52, 64, 515, 775, 823
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 1 cut(s) 387
BclI TGATCA 3 cut(s) 466, 568, 826
BcoDI GTCTC 2 cut(s) 76, 681
BfaI CTAG 2 cut(s) 683, 762
BfmI CTRYAG 2 cut(s) 32, 169
BisI GCNGC 5 cut(s) 259, 262, 265, 277, 288
BlpI GCTNAGC 1 cut(s) 502
BlsI GCNGC 5 cut(s) 260, 263, 266, 278, 289
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 720
BmgT120I GGNCC 1 cut(s) 720
BmiI GGNNCC 2 cut(s) 232, 722
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 343, 410, 718
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 86, 185, 277, 540
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 254, 487
Bse1I ACTGG 1 cut(s) 248
Bse8I GATNNNNATC 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 86, 185, 277, 540
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 3 cut(s) 106, 254, 487
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 226
Bsh1236I CGCG 2 cut(s) 279, 312
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 2 cut(s) 230, 706
BslI CCNNNNNNNGG 3 cut(s) 106, 254, 487
BsmAI GTCTC 2 cut(s) 76, 681
BsmBI CGTCTC 1 cut(s) 681
BsmFI GGGAC 2 cut(s) 230, 706
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 872
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
Bsp1720I GCTNAGC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 7 cut(s) 258, 265, 274, 277, 279, 287, 310
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 515
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 279, 312
BspHI TCATGA 3 cut(s) 63, 475, 658
BspLI GGNNCC 2 cut(s) 232, 722
BspPI GGATC 3 cut(s) 5, 359, 400
BspTNI GGTCTC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 4 cut(s) 86, 185, 277, 540
BssMI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
BssNI GRCGYC 1 cut(s) 737
BssT1I CCWWGG 3 cut(s) 86, 185, 540
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 3 cut(s) 19, 36, 806
Bst6I CTCTTC 2 cut(s) 74, 142
BstACI GRCGYC 1 cut(s) 737
BstAPI GCANNNNNTGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 502
BstDSI CCRYGG 2 cut(s) 86, 277
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 2 cut(s) 279, 312
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 9 cut(s) 13, 367, 395, 469, 517, 553, 571, 829, 908
BstMAI GTCTC 2 cut(s) 76, 681
BstMBI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
BstMWI GCNNNNNNNGC 4 cut(s) 204, 237, 264, 273
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 2 cut(s) 32, 169
BstUI CGCG 2 cut(s) 279, 312
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 2 cut(s) 10, 392
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 2 cut(s) 10, 392
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 2 cut(s) 86, 277
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 41
CciI TCATGA 3 cut(s) 63, 475, 658
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 720
Cfr42I CCGCGG 1 cut(s) 280
Csp6I GTAC 3 cut(s) 190, 354, 756
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 64, 87, 476, 659, 725, 771
CviQI GTAC 3 cut(s) 190, 354, 756
DdeI CTNAG 1 cut(s) 502
DpnI GATC 9 cut(s) 12, 366, 394, 468, 516, 552, 570, 828, 907
DpnII GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 74, 142
EarI CTCTTC 2 cut(s) 74, 142
Ecl136II GAGCTC 1 cut(s) 870
Eco130I CCWWGG 3 cut(s) 86, 185, 540
Eco24I GRGCYC 1 cut(s) 872
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 720
Eco53kI GAGCTC 1 cut(s) 870
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 870
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 3 cut(s) 86, 185, 540
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 872
ErhI CCWWGG 3 cut(s) 86, 185, 540
Esp3I CGTCTC 1 cut(s) 681
FaeI CATG 6 cut(s) 67, 90, 479, 662, 728, 774
FaqI GGGAC 2 cut(s) 230, 706
FatI CATG 6 cut(s) 63, 86, 475, 658, 724, 770
FbaI TGATCA 3 cut(s) 466, 568, 826
Fnu4HI GCNGC 5 cut(s) 259, 262, 265, 277, 288
FokI GGATG 1 cut(s) 27
FriOI GRGCYC 1 cut(s) 872
Fsp4HI GCNGC 5 cut(s) 259, 262, 265, 277, 288
FspBI CTAG 2 cut(s) 683, 762
GlaI GCGC 1 cut(s) 313
GluI GCNGC 5 cut(s) 259, 262, 265, 277, 288
HaeIII GGCC 1 cut(s) 290
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 737
Hin1II CATG 6 cut(s) 67, 90, 479, 662, 728, 774
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HinfI GANTC 1 cut(s) 294
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 3 cut(s) 6, 680, 809
Hpy188I TCNGA 2 cut(s) 406, 820
Hpy188III TCNNGA 6 cut(s) 64, 372, 476, 518, 659, 903
Hpy8I GTNNAC 3 cut(s) 6, 680, 809
Hpy99I CGWCG 2 cut(s) 739, 742
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 3 cut(s) 19, 36, 806
HpyCH4IV ACGT 3 cut(s) 243, 620, 737
HpyCH4V TGCA 7 cut(s) 198, 207, 401, 706, 731, 787, 793
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 237, 264, 273
HpyF3I CTNAG 1 cut(s) 502
HpySE526I ACGT 3 cut(s) 243, 620, 737
Hsp92I GRCGYC 1 cut(s) 737
Hsp92II CATG 6 cut(s) 67, 90, 479, 662, 728, 774
HspAI GCGC 1 cut(s) 312
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 3 cut(s) 466, 568, 826
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
LmnI GCTCC 3 cut(s) 236, 867, 875
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 3 cut(s) 343, 410, 718
MaeI CTAG 2 cut(s) 683, 762
MaeII ACGT 3 cut(s) 243, 620, 737
MaeIII GTNAC 2 cut(s) 323, 669
MalI GATC 9 cut(s) 12, 366, 394, 468, 516, 552, 570, 828, 907
MboI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
MboII GAAGA 7 cut(s) 91, 129, 371, 528, 617, 704, 847
MfeI CAATTG 1 cut(s) 862
MflI RGATCY 2 cut(s) 10, 392
MhlI GDGCHC 1 cut(s) 872
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 2 cut(s) 165, 780
MnlI CCTC 5 cut(s) 100, 135, 145, 185, 221
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 5 cut(s) 21, 320, 440, 633, 910
MslI CAYNNNNRTG 3 cut(s) 68, 474, 642
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 1 cut(s) 862
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 2 cut(s) 279, 312
MwoI GCNNNNNNNGC 4 cut(s) 204, 237, 264, 273
NcoI CCATGG 1 cut(s) 86
NdeII GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
NlaIII CATG 6 cut(s) 67, 90, 479, 662, 728, 774
NlaIV GGNNCC 2 cut(s) 232, 722
NsiI ATGCAT 1 cut(s) 403
PagI TCATGA 3 cut(s) 63, 475, 658
PdmI GAANNNNTTC 1 cut(s) 612
PflFI GACNNNGTC 1 cut(s) 739
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 5 cut(s) 260, 263, 266, 278, 289
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 872
Psp1406I AACGTT 1 cut(s) 620
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 232, 722
PspPI GGNCC 1 cut(s) 720
PsuI RGATCY 2 cut(s) 10, 392
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 3 cut(s) 191, 355, 757
RsaNI GTAC 3 cut(s) 190, 354, 756
RseI CAYNNNNRTG 3 cut(s) 68, 474, 642
SacI GAGCTC 1 cut(s) 872
SacII CCGCGG 1 cut(s) 280
SaqAI TTAA 5 cut(s) 21, 320, 440, 633, 910
SatI GCNGC 5 cut(s) 259, 262, 265, 277, 288
Sau3AI GATC 9 cut(s) 10, 364, 392, 466, 514, 550, 568, 826, 905
Sau96I GGNCC 1 cut(s) 720
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 872
SetI ASST 8 cut(s) 111, 191, 246, 391, 503, 623, 740, 872
SfaNI GCATC 3 cut(s) 343, 410, 718
SfcI CTRYAG 2 cut(s) 32, 169
Sfr303I CCGCGG 1 cut(s) 280
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 1 cut(s) 720
SmiMI CAYNNNNRTG 3 cut(s) 68, 474, 642
SsiI CCGC 7 cut(s) 258, 265, 274, 277, 279, 287, 310
SspMI CTAG 2 cut(s) 683, 762
SstI GAGCTC 1 cut(s) 872
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 3 cut(s) 86, 185, 540
TaaI ACNGT 3 cut(s) 19, 36, 806
TaiI ACGT 3 cut(s) 246, 623, 740
TaqI TCGA 2 cut(s) 127, 842
TatI WGTACW 1 cut(s) 755
TauI GCSGC 4 cut(s) 261, 267, 279, 290
Tru1I TTAA 5 cut(s) 21, 320, 440, 633, 910
Tru9I TTAA 5 cut(s) 21, 320, 440, 633, 910
TscAI CASTG 1 cut(s) 41
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 7 cut(s) 52, 92, 306, 647, 665, 675, 848
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 41
Tth111I GACNNNGTC 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 720
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 4 cut(s) 149, 434, 532, 662
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 683, 762
ZraI GACGTC 1 cut(s) 738
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.