MD10G1017800.v1.1

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015373
Physical Location & Seq
Reverse (-)
2245517 .. 2246428
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1017800.v1.1.491

Sequence Viewer

Length: 912 bp
ATGTTCATAGCTCCTAGCGTTAAACAAATACTACAATGCCCTCCCTCTGGGCAAGAGCAATTCATGATGATGGAGATGAAGACACCATGGAACAAATCCCATATTGAGATTGCTCCAAACTGCCCTCGATGTGCCTCTTCCAACACAAAATTTTGCTACTACAACAACTACAGCCTCTCACAGCCAAGGTACTTCTGCAAAGGGTGCAGGAGATATTGGACTAAAGGCGGCTCCTTACGCAACGTCCCCGTTGGTGGTGGCTGCCGCAAGAACCGCCGTGGTAAGGCAGCCAGGCTCTCACAGGCTGACCGCGGTTCATTAAGTTATTTTCAGCATCACAACTCATCAACTACTACTGAAGATGATCTGGCCGGAAATACTCAGCCAGGTGGATCTAATGTACCAGATATTGATTTAGCTGTGGTGTTTGCCAAATTCTTGAACCACAATTCCACTACCCCTGATGGTCATGACCATGACCCCAACTTGGTAATTTCAAGTTCAGCATTGAACGATCGTGACGGCTCGCATAATTCTTCAAAGGCTGCTGATGATCTTGTTGAAGCAGTTGGTCATCATTATTTACTTGAAGGACATCAATCACAAGAAGAAAATGTTCAAACTTTTATGGGAATTAATCACAATGATGATATGAACATTCATGAATTTGAGTTACAGGGTTTACTAGGCGATGATGAACAAGTGGTGCAAGATGTGTTTTGGTCCGATGATGCAACGACGTCTTTGACGAGCAGCACTGCTAGTTTTACATGGCAACCTATGATGGATCTGCAAGAGTTGGACTGTTCACTACCATCCGACGATGATCAAATGAAGATATCAACCAATTTGTGTGGCGATAATTGGAGCTCCTTTGAATTTTCAGGGTTTGAAGTTTTCTCACGATCTTAA

Protein Analysis

304

Amino Acids

33.8

Weight (kDa)

5.04

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 39 - 93 4e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21340
fragaria_vesca FvH4_2g14390
malus_domestica MD05G1018200.v1.1 MD10G1017800.v1.1
prunus_persica Prupe.8G022200_v2.0.a1
pyrus_communis pycom05g01080
rosa_chinensis RchiOBHm_Chr6g0276721
rosa_laevigata RLG00000013370
rosa_multiflora Rmu_sc0000263.1_g000003
rosa_roxburghii Rroxscaffold_7G00192050
rosa_rugosa Rorug06G0103100
rosa_samantha Rh6AG213500 Rh6BG218400 Rh6CG221200 Rh6DG211100
rosa_wichuraiana Rw6G018670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 743
AccII CGCG 1 cut(s) 312
AciI CCGC 5 cut(s) 228, 265, 274, 310, 312
AclWI GGATC 2 cut(s) 400, 795
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 4 cut(s) 149, 434, 665, 878
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 740
AfaI GTAC 2 cut(s) 191, 402
AfiI CCNNNNNNNGG 4 cut(s) 47, 254, 283, 487
AgsI TTSAA 9 cut(s) 442, 498, 511, 540, 563, 590, 620, 878, 893
AjnI CCWGG 2 cut(s) 290, 385
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 3 cut(s) 11, 419, 870
AluI AGCT 3 cut(s) 11, 419, 870
Alw21I GWGCWC 1 cut(s) 872
AlwI GGATC 2 cut(s) 400, 795
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 4 cut(s) 261, 287, 545, 753
ApoI RAATTY 4 cut(s) 149, 434, 665, 878
AseI ATTAAT 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 615
AspS9I GGNCC 1 cut(s) 723
AvaII GGWCC 1 cut(s) 723
BanII GRGCYC 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 872
BbvI GCAGC 4 cut(s) 248, 299, 532, 765
BccI CCATC 4 cut(s) 64, 458, 778, 823
BceAI ACGGC 2 cut(s) 261, 538
BciT130I CCWGG 2 cut(s) 292, 387
BclI TGATCA 1 cut(s) 826
BfaI CTAG 3 cut(s) 15, 686, 762
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 6 cut(s) 229, 262, 265, 288, 546, 754
BlsI GCNGC 6 cut(s) 230, 263, 266, 289, 547, 755
Bme1390I CCNGG 2 cut(s) 292, 387
Bme18I GGWCC 1 cut(s) 723
BmgT120I GGNCC 1 cut(s) 723
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 292, 387
BmsI GCATC 2 cut(s) 343, 721
BpiI GAAGAC 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 740
BsaJI CCNNGG 4 cut(s) 86, 185, 277, 310
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 254, 283, 487
BseBI CCWGG 2 cut(s) 292, 387
BseDI CCNNGG 4 cut(s) 86, 185, 277, 310
BseGI GGATG 1 cut(s) 815
BseLI CCNNNNNNNGG 4 cut(s) 47, 254, 283, 487
BseMII CTCAG 1 cut(s) 395
BseXI GCAGC 4 cut(s) 248, 299, 532, 765
BsgI GTGCAG 1 cut(s) 226
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 517
BshFI GGCC 1 cut(s) 371
BsiEI CGRYCG 1 cut(s) 517
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 4 cut(s) 47, 254, 283, 487
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 872
Bsp143I GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 5 cut(s) 228, 265, 274, 310, 312
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 1 cut(s) 312
BspHI TCATGA 3 cut(s) 63, 469, 661
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 2 cut(s) 400, 795
BssECI CCNNGG 4 cut(s) 86, 185, 277, 310
BssMI GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
BssNI GRCGYC 1 cut(s) 740
BssT1I CCWWGG 2 cut(s) 86, 185
Bst2UI CCWGG 2 cut(s) 292, 387
Bst4CI ACNGT 1 cut(s) 806
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 381
BstDSI CCRYGG 3 cut(s) 86, 277, 310
BstF5I GGATG 1 cut(s) 815
BstFNI CGCG 1 cut(s) 312
BstKTI GATC 7 cut(s) 367, 395, 517, 556, 790, 829, 908
BstMBI GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
BstMCI CGRYCG 1 cut(s) 517
BstMWI GCNNNNNNNGC 3 cut(s) 204, 237, 273
BstNI CCWGG 2 cut(s) 292, 387
BstSCI CCNGG 2 cut(s) 290, 385
BstSFI CTRYAG 1 cut(s) 169
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 248, 299, 532, 765
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 392, 787
BstYI RGATCY 2 cut(s) 392, 787
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 3 cut(s) 86, 277, 310
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 815
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 1 cut(s) 527
CciI TCATGA 3 cut(s) 63, 469, 661
Cfr13I GGNCC 1 cut(s) 723
Cfr42I CCGCGG 1 cut(s) 313
Csp6I GTAC 2 cut(s) 190, 401
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 64, 87, 470, 476, 662, 771
CviQI GTAC 2 cut(s) 190, 401
DdeI CTNAG 1 cut(s) 381
DpnI GATC 7 cut(s) 366, 394, 516, 555, 789, 828, 907
DpnII GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 870
Eco130I CCWWGG 2 cut(s) 86, 185
Eco24I GRGCYC 1 cut(s) 872
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 723
Eco53kI GAGCTC 1 cut(s) 870
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 870
EcoRII CCWGG 2 cut(s) 290, 385
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 2 cut(s) 86, 185
EcoT38I GRGCYC 1 cut(s) 872
ErhI CCWWGG 2 cut(s) 86, 185
FaeI CATG 6 cut(s) 67, 90, 473, 479, 665, 774
FaqI GGGAC 1 cut(s) 230
FatI CATG 6 cut(s) 63, 86, 469, 475, 661, 770
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 6 cut(s) 229, 262, 265, 288, 546, 754
FokI GGATG 1 cut(s) 802
FriOI GRGCYC 1 cut(s) 872
Fsp4HI GCNGC 6 cut(s) 229, 262, 265, 288, 546, 754
FspBI CTAG 3 cut(s) 15, 686, 762
GluI GCNGC 6 cut(s) 229, 262, 265, 288, 546, 754
HaeIII GGCC 1 cut(s) 371
HapII CCGG 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 740
Hin1II CATG 6 cut(s) 67, 90, 473, 479, 665, 774
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 2 cut(s) 683, 809
Hpy188I TCNGA 2 cut(s) 727, 820
Hpy188III TCNNGA 6 cut(s) 64, 439, 470, 518, 662, 903
Hpy8I GTNNAC 2 cut(s) 683, 809
Hpy99I CGWCG 2 cut(s) 742, 824
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 1 cut(s) 806
HpyCH4IV ACGT 2 cut(s) 243, 740
HpyCH4V TGCA 5 cut(s) 198, 207, 709, 734, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 237, 273
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 2 cut(s) 243, 740
Hsp92I GRCGYC 1 cut(s) 740
Hsp92II CATG 6 cut(s) 67, 90, 473, 479, 665, 774
Ksp22I TGATCA 1 cut(s) 826
KspI CCGCGG 1 cut(s) 313
Kzo9I GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
LmnI GCTCC 5 cut(s) 16, 118, 236, 867, 875
Lsp1109I GCAGC 4 cut(s) 248, 299, 532, 765
LweI GCATC 2 cut(s) 343, 721
MaeI CTAG 3 cut(s) 15, 686, 762
MaeII ACGT 2 cut(s) 243, 740
MaeIII GTNAC 2 cut(s) 518, 672
MalI GATC 7 cut(s) 366, 394, 516, 555, 789, 828, 907
MboI GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
MboII GAAGA 6 cut(s) 91, 129, 371, 528, 620, 847
MflI RGATCY 2 cut(s) 392, 787
MhlI GDGCHC 1 cut(s) 872
MmeI TCCRAC 3 cut(s) 165, 780, 843
MnlI CCTC 5 cut(s) 51, 55, 135, 145, 185
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 4 cut(s) 21, 320, 636, 910
MslI CAYNNNNRTG 3 cut(s) 68, 474, 645
MspA1I CMGCKG 1 cut(s) 312
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 292, 387
MvaI CCWGG 2 cut(s) 292, 387
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 204, 237, 273
NcoI CCATGG 1 cut(s) 86
NdeII GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
NlaIII CATG 6 cut(s) 67, 90, 473, 479, 665, 774
NlaIV GGNNCC 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 518
PagI TCATGA 3 cut(s) 63, 469, 661
PcsI WCGNNNNNNNCGW 1 cut(s) 746
PdmI GAANNNNTTC 1 cut(s) 615
PkrI GCNGC 6 cut(s) 230, 263, 266, 289, 547, 755
Ple19I CGATCG 1 cut(s) 517
PshBI ATTAAT 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 872
Psp6I CCWGG 2 cut(s) 290, 385
PspGI CCWGG 2 cut(s) 290, 385
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 723
PsuI RGATCY 2 cut(s) 392, 787
PvuI CGATCG 1 cut(s) 517
RsaI GTAC 2 cut(s) 191, 402
RsaNI GTAC 2 cut(s) 190, 401
RseI CAYNNNNRTG 3 cut(s) 68, 474, 645
SacI GAGCTC 1 cut(s) 872
SacII CCGCGG 1 cut(s) 313
SaqAI TTAA 4 cut(s) 21, 320, 636, 910
SatI GCNGC 6 cut(s) 229, 262, 265, 288, 546, 754
Sau3AI GATC 7 cut(s) 364, 392, 514, 553, 787, 826, 905
Sau96I GGNCC 1 cut(s) 723
ScrFI CCNGG 2 cut(s) 292, 387
SduI GDGCHC 1 cut(s) 872
SetI ASST 8 cut(s) 13, 191, 246, 391, 421, 743, 781, 872
SfaNI GCATC 2 cut(s) 343, 721
SfcI CTRYAG 1 cut(s) 169
Sfr303I CCGCGG 1 cut(s) 313
SgrBI CCGCGG 1 cut(s) 313
SinI GGWCC 1 cut(s) 723
SmiMI CAYNNNNRTG 3 cut(s) 68, 474, 645
SsiI CCGC 5 cut(s) 228, 265, 274, 310, 312
SspMI CTAG 3 cut(s) 15, 686, 762
SstI GAGCTC 1 cut(s) 872
StyD4I CCNGG 2 cut(s) 290, 385
StyI CCWWGG 2 cut(s) 86, 185
TaaI ACNGT 1 cut(s) 806
TaiI ACGT 2 cut(s) 246, 743
TaqI TCGA 1 cut(s) 127
TauI GCSGC 2 cut(s) 231, 267
Tru1I TTAA 4 cut(s) 21, 320, 636, 910
Tru9I TTAA 4 cut(s) 21, 320, 636, 910
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 4 cut(s) 261, 287, 545, 753
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 8 cut(s) 52, 92, 306, 650, 668, 678, 711, 848
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 723
VspI ATTAAT 1 cut(s) 636
XapI RAATTY 4 cut(s) 149, 434, 665, 878
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 3 cut(s) 15, 686, 762
ZraI GACGTC 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.