Rh6DG211100

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0015373
Physical Location & Seq
Forward (+)
37619391 .. 37620771
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG211100.1

Sequence Viewer

Length: 900 bp
ATGTTCACAGCTCCAGTTCTCGAACAGATGCTGCACTCTCAATCCGGACAATTGGTCATGAACCAGAAGCCGTCGTGGAAGCCCCACGTCGAGATCGCTCCCAATTGCCCCCGCTGCGCGTCTTCCAACACCAAGTTCTGCTACTACAACAACTACAGCCTCTCGCAGCCGCGCTACTTCTGCAAAGGCTGCCGGAGATACTGGACCAAAGGCGGGTCCCTGAGAAACGTGCCCGTCGGCGGCGGTTGCAGAAAGAACCGCCGCGGCAGGTCGGTCAGGCTCTCGCAGGCTGACCGGGTTTCGTTGAGTTACTATCGTCACAACAGCTCGAGTGGTAGCGGTGGTGATCTATCCGAGCAGCAGTCCATGGAAGGTGGAGCCAACGGATCGGATATCGACTTGGCTGCTGTGTTTGCTAAGTTCTTGAACCAAGACTCTAGCAACGAACATGATCCCAATTTGGTTGGATCAGAATCGATCCCCGACGATGTTCATGAGGCAAACGCATTATCTGATCACTCCCAAATTTCTTCAAACCCCGAGGAAGATCTTCTTGAATCAGTGGAGCTACTTGAAGGACTCGTACTTCCTGATCAGGATCATCAACAATTACACGAAGAGGATAATATTCAGAATATTATGGGAAATCAACACCATCATGATTTGAGTATTCATGAATTTGGGTTGCAGGGTTTGCTAGGCGATGAGGATGTGTTTTGGACTGATACAACTGCAAGTTTGACAAGTAGTACTACTAGTTTCACATGGCAAGAGTTGGATAATTCATTCCCATCTAATGATGATGATCATATGAAGATATCGACCACCACGACGAATCTATGTAGCGATAACTGGAGCTCCTTTGATTTTTCAGGCTTGGAAGTTTTCTCAGGATCATAA

Protein Analysis

299

Amino Acids

33.22

Weight (kDa)

4.82

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 34 - 88 3.9e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21340
fragaria_vesca FvH4_2g14390
malus_domestica MD05G1018200.v1.1 MD10G1017800.v1.1
prunus_persica Prupe.8G022200_v2.0.a1
pyrus_communis pycom05g01080
rosa_chinensis RchiOBHm_Chr6g0276721
rosa_laevigata RLG00000013370
rosa_multiflora Rmu_sc0000263.1_g000003
rosa_roxburghii Rroxscaffold_7G00192050
rosa_rugosa Rorug06G0103100
rosa_samantha Rh6AG213500 Rh6BG218400 Rh6CG221200 Rh6DG211100
rosa_wichuraiana Rw6G018670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 258
AccII CGCG 3 cut(s) 119, 172, 264
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 9 cut(s) 112, 170, 213, 240, 243, 259, 262, 264, 339
AclWI GGATC 5 cut(s) 394, 446, 472, 475, 606
AcsI RAATTY 2 cut(s) 525, 677
AfaI GTAC 2 cut(s) 585, 751
AfiI CCNNNNNNNGG 2 cut(s) 213, 239
AgsI TTSAA 4 cut(s) 427, 534, 557, 575
AhdI GACNNNNNGTC 1 cut(s) 53
AhlI ACTAGT 1 cut(s) 755
AjiI CACGTC 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 4 cut(s) 11, 327, 568, 858
AluI AGCT 4 cut(s) 11, 327, 568, 858
Alw21I GWGCWC 1 cut(s) 860
AlwI GGATC 5 cut(s) 394, 446, 472, 475, 606
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 2 cut(s) 328, 539
Aor13HI TCCGGA 1 cut(s) 44
ApeKI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
ApoI RAATTY 2 cut(s) 525, 677
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 2 cut(s) 119, 174
AspS9I GGNCC 2 cut(s) 204, 216
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 356
AvaI CYCGRG 2 cut(s) 328, 539
AvaII GGWCC 2 cut(s) 204, 216
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 860
BarI GAAGNNNNNNTAC 2 cut(s) 567, 599
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 860
BbvI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BccI CCATC 2 cut(s) 663, 799
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BclI TGATCA 3 cut(s) 514, 592, 805
BcnI CCSGG 1 cut(s) 296
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 3 cut(s) 438, 698, 756
BfmI CTRYAG 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 258
BglII AGATCT 1 cut(s) 547
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 204, 216
BmeRI GACNNNNNGTC 1 cut(s) 53
BmeT110I CYCGRG 2 cut(s) 328, 539
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 204, 216
BmiI GGNNCC 3 cut(s) 217, 218, 379
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 874
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 476
BsaBI GATNNNNATC 3 cut(s) 472, 597, 804
BsaJI CCNNGG 3 cut(s) 262, 366, 540
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 239
Bse1I ACTGG 3 cut(s) 14, 206, 857
Bse8I GATNNNNATC 3 cut(s) 472, 597, 804
BseAI TCCGGA 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 3 cut(s) 262, 366, 540
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 3 cut(s) 472, 597, 804
BseLI CCNNNNNNNGG 2 cut(s) 213, 239
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 3 cut(s) 14, 206, 857
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BsgI GTGCAG 1 cut(s) 17
Bsh1236I CGCG 3 cut(s) 119, 172, 264
BshVI ATCGAT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 860
BsiHKCI CYCGRG 2 cut(s) 328, 539
BsiSI CCGG 3 cut(s) 45, 193, 295
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 213, 239
BsmFI GGGAC 1 cut(s) 202
BsoBI CYCGRG 2 cut(s) 328, 539
Bsp1286I GDGCHC 2 cut(s) 234, 860
Bsp13I TCCGGA 1 cut(s) 44
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 9 cut(s) 112, 170, 213, 240, 243, 259, 262, 264, 339
BspCNI CTCAG 1 cut(s) 213
BspDI ATCGAT 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 44
BspFNI CGCG 3 cut(s) 119, 172, 264
BspHI TCATGA 4 cut(s) 57, 493, 658, 673
BspLI GGNNCC 3 cut(s) 217, 218, 379
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 5 cut(s) 394, 446, 472, 475, 606
BsrI ACTGG 3 cut(s) 14, 206, 857
BssECI CCNNGG 3 cut(s) 262, 366, 540
BssT1I CCWWGG 1 cut(s) 366
Bst6I CTCTTC 1 cut(s) 612
BstAPI GCANNNNNTGC 2 cut(s) 189, 694
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 3 cut(s) 221, 417, 889
BstDSI CCRYGG 2 cut(s) 262, 366
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 3 cut(s) 119, 172, 264
BstHHI GCGC 2 cut(s) 119, 174
BstMWI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 694
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 3 cut(s) 119, 172, 264
BstV1I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 476
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 2 cut(s) 262, 366
BtgZI GCGATG 1 cut(s) 717
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 4 cut(s) 57, 493, 658, 673
CfoI GCGC 2 cut(s) 119, 174
Cfr13I GGNCC 2 cut(s) 204, 216
Cfr42I CCGCGG 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 108
Csp6I GTAC 2 cut(s) 584, 750
CviAII CATG 7 cut(s) 58, 367, 449, 494, 659, 674, 765
CviQI GTAC 2 cut(s) 584, 750
DdeI CTNAG 3 cut(s) 221, 417, 889
DriI GACNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 860
Eco32I GATATC 2 cut(s) 394, 819
Eco47I GGWCC 2 cut(s) 204, 216
Eco53kI GAGCTC 1 cut(s) 858
Eco88I CYCGRG 2 cut(s) 328, 539
EcoICRI GAGCTC 1 cut(s) 858
EcoO109I RGGNCCY 1 cut(s) 216
EcoRV GATATC 2 cut(s) 394, 819
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 7 cut(s) 61, 370, 452, 497, 662, 677, 768
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 202
FatI CATG 7 cut(s) 57, 366, 448, 493, 658, 673, 764
FauI CCCGC 2 cut(s) 119, 206
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 3 cut(s) 514, 592, 805
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 1 cut(s) 860
FspBI CTAG 3 cut(s) 438, 698, 756
GlaI GCGC 2 cut(s) 118, 173
GsuI CTGGAG 1 cut(s) 874
HapII CCGG 3 cut(s) 45, 193, 295
HgaI GACGC 1 cut(s) 108
HhaI GCGC 2 cut(s) 119, 174
Hin1II CATG 7 cut(s) 61, 370, 452, 497, 662, 677, 768
Hin6I GCGC 2 cut(s) 117, 172
HinP1I GCGC 2 cut(s) 117, 172
HinfI GANTC 5 cut(s) 434, 473, 557, 579, 835
HpaII CCGG 3 cut(s) 45, 193, 295
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 5 cut(s) 355, 391, 472, 514, 633
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 5 cut(s) 76, 92, 239, 488, 835
HpyAV CCTTC 2 cut(s) 365, 569
HpyCH4IV ACGT 2 cut(s) 87, 228
HpyCH4V TGCA 5 cut(s) 34, 183, 249, 688, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 694
HpyF3I CTNAG 3 cut(s) 221, 417, 889
HpySE526I ACGT 2 cut(s) 87, 228
Hsp92II CATG 7 cut(s) 61, 370, 452, 497, 662, 677, 768
HspAI GCGC 2 cut(s) 117, 172
KflI GGGWCCC 1 cut(s) 216
Kpn2I TCCGGA 1 cut(s) 44
Ksp22I TGATCA 3 cut(s) 514, 592, 805
KspI CCGCGG 1 cut(s) 265
LmnI GCTCC 6 cut(s) 16, 103, 377, 565, 855, 863
Lsp1109I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
LweI GCATC 1 cut(s) 18
MaeI CTAG 3 cut(s) 438, 698, 756
MaeII ACGT 2 cut(s) 87, 228
MaeIII GTNAC 2 cut(s) 308, 317
MboII GAAGA 6 cut(s) 114, 522, 542, 557, 629, 826
MfeI CAATTG 2 cut(s) 50, 103
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 2 cut(s) 234, 860
MluCI AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
MlyI GAGTC 2 cut(s) 428, 573
MmeI TCCRAC 3 cut(s) 150, 445, 756
MnlI CCTC 5 cut(s) 170, 490, 535, 613, 700
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 2 cut(s) 114, 264
MspI CCGG 3 cut(s) 45, 193, 295
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 2 cut(s) 50, 103
MvnI CGCG 3 cut(s) 119, 172, 264
MwoI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 694
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 810
NlaIII CATG 7 cut(s) 61, 370, 452, 497, 662, 677, 768
NlaIV GGNNCC 3 cut(s) 217, 218, 379
NmuCI GTSAC 1 cut(s) 317
PaeR7I CTCGAG 1 cut(s) 328
PagI TCATGA 4 cut(s) 57, 493, 658, 673
PcsI WCGNNNNNNNCGW 1 cut(s) 827
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 473, 557, 835
PleI GAGTC 2 cut(s) 428, 573
PpsI GAGTC 2 cut(s) 428, 573
PpuMI RGGWCCY 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 860
Psp5II RGGWCCY 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 217, 218, 379
PspPI GGNCC 2 cut(s) 204, 216
PspPPI RGGWCCY 1 cut(s) 216
PspXI VCTCGAGB 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 2 cut(s) 585, 751
RsaNI GTAC 2 cut(s) 584, 750
RseI CAYNNNNRTG 1 cut(s) 657
SacI GAGCTC 1 cut(s) 860
SacII CCGCGG 1 cut(s) 265
Sau96I GGNCC 2 cut(s) 204, 216
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 2 cut(s) 428, 573
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 234, 860
SetI ASST 8 cut(s) 13, 90, 231, 272, 329, 376, 570, 860
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 328
Sfr303I CCGCGG 1 cut(s) 265
SgrBI CCGCGG 1 cut(s) 265
SinI GGWCC 2 cut(s) 204, 216
SlaI CTCGAG 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
SsiI CCGC 9 cut(s) 112, 170, 213, 240, 243, 259, 262, 264, 339
SspI AATATT 2 cut(s) 628, 637
SspMI CTAG 3 cut(s) 438, 698, 756
SstI GAGCTC 1 cut(s) 860
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 366
TaiI ACGT 2 cut(s) 90, 231
TaqI TCGA 6 cut(s) 21, 90, 329, 396, 476, 821
TaqII GACCGA 1 cut(s) 262
TasI AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
TatI WGTACW 1 cut(s) 749
TauI GCSGC 4 cut(s) 172, 243, 264, 267
TfiI GAWTC 3 cut(s) 473, 557, 835
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 6 cut(s) 74, 482, 662, 690, 774, 827
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 204, 216
XapI RAATTY 2 cut(s) 525, 677
XhoI CTCGAG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 3 cut(s) 438, 698, 756
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.