MD05G1033000.v1.1

conserved protein UCP009193

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013109
Physical Location & Seq
Reverse (-)
5356325 .. 5359893
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1033000.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGGGAAGAAGAGAAAGTCGGTGGCCACCAGCCTCGACGAGGTTGATCGAAGCATGTATACCTCCTTCTGCAGCGCCGCTAATTCCCTCTCTCAGCTCTACACTCAGGCCATGAACCACCAAAAACTCTCCTTTCAAGCCGGTGAACGCCACGCCCTTGATAAAATTTATCAGTGGATCTGTAGACAACAAGAAGGAGGCTCACGAGTTACAACAGTCGATATAGTAAATTATCTTCAGAATGAGTTGGACTATTGTGGAGAGGAGCCATCAATGTCCCCTAGGTTGCCACTCCAACATCAGCATCTGCAGCCCACTGCGCATTTCTCAAACTCAGGTTTGCCAGTTACTTCTGGTTCATCTGGTCCAACAAATACTGGGCAGGGAATTCGTTCTGAGCAGTGCGATCATCAATCTAAGAATTCGGTCTTCTCAAATGCTTTGTCGAGCCCTGTTCGCCAGAGTCTTCAGCACTATCACATTTCTCAAGATGGATATTATCCAGGAGCAGTTCTGCCATCTGGAAATGGAGCCCGAAACAATGAACCTAGCTTTCTCCACCCAAACCGGGATGCTAATCCTCCAAGTTCCAATGATACCTCGATGGACATGCATGCAGATAGTCCTGGTCATGACTCTACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4588

Protein Analysis

216

Amino Acids

23.67

Weight (kDa)

6.23

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAPR1-like PF15251 15 - 204 1.3e-07 Telomere attrition and p53 response 1 protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09830
fragaria_vesca FvH4_2g13040
malus_domestica MD05G1033000.v1.1 MD10G1034000.v1.1
prunus_persica Prupe.8G040300_v2.0.a1
pyrus_communis pycom05g02400 pycom10g02250
rosa_chinensis RchiOBHm_Chr6g0274261
rosa_laevigata RLG00000013560
rosa_multiflora Rmu_sc0005132.1_g000023 Rmu_sc0007877.1_g000001
rosa_roxburghii Rroxscaffold_7G00194380
rosa_rugosa Rorug06G0080700.1
rosa_samantha Rh6AG195300 Rh6BG199900 Rh6CG199800 Rh6DG191300
rosa_wichuraiana Rw6G017090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 321
AccI GTMKAC 2 cut(s) 59, 184
AciI CCGC 1 cut(s) 78
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 3 cut(s) 165, 387, 421
AcuI CTGAAG 2 cut(s) 221, 452
AfiI CCNNNNNNNGG 2 cut(s) 40, 568
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 2 cut(s) 502, 625
AluBI AGCT 2 cut(s) 97, 552
AluI AGCT 2 cut(s) 97, 552
AlwI GGATC 1 cut(s) 185
AlwNI CAGNNNCTG 1 cut(s) 307
AoxI GGCC 2 cut(s) 24, 108
ApeKI GCWGC 2 cut(s) 72, 310
ApoI RAATTY 3 cut(s) 165, 387, 421
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
Asp700I GAANNNNTTC 1 cut(s) 391
AspA2I CCTAGG 1 cut(s) 281
AspLEI GCGC 2 cut(s) 77, 322
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 365
AvrII CCTAGG 1 cut(s) 281
BalI TGGCCA 1 cut(s) 26
BanII GRGCYC 2 cut(s) 452, 535
BarI GAAGNNNNNNTAC 6 cut(s) 43, 50, 75, 82, 219, 251
BauI CACGAG 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 421, 458
BbvI GCAGC 2 cut(s) 84, 322
BccI CCATC 4 cut(s) 277, 485, 526, 598
BcgI CGANNNNNNTGC 4 cut(s) 371, 405, 592, 626
BciT130I CCWGG 2 cut(s) 504, 627
BcnI CCSGG 1 cut(s) 569
BfaI CTAG 2 cut(s) 282, 549
BfmI CTRYAG 3 cut(s) 70, 181, 308
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 3 cut(s) 73, 78, 311
BlnI CCTAGG 1 cut(s) 281
BlsI GCNGC 3 cut(s) 74, 79, 312
Bme1390I CCNGG 3 cut(s) 504, 569, 627
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 2 cut(s) 267, 532
BmrFI CCNGG 3 cut(s) 504, 569, 627
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 313, 562
BmuI ACTGGG 1 cut(s) 387
BpiI GAAGAC 2 cut(s) 421, 458
BpuEI CTTGAG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 1 cut(s) 281
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 568
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 344, 382
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 2 cut(s) 504, 627
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 577
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 40, 568
BseMII CTCAG 4 cut(s) 107, 119, 348, 387
BseNI ACTGG 2 cut(s) 344, 382
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 84, 322
BshFI GGCC 2 cut(s) 26, 110
BsiSI CCGG 2 cut(s) 141, 568
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 40, 568
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 2 cut(s) 26, 110
Bsp1286I GDGCHC 2 cut(s) 452, 535
Bsp143I GATC 3 cut(s) 46, 177, 406
BspACI CCGC 1 cut(s) 78
BspANI GGCC 2 cut(s) 26, 110
BspCNI CTCAG 4 cut(s) 106, 118, 347, 388
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 267, 532
BspMAI CTGCAG 2 cut(s) 74, 312
BspPI GGATC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 344, 382
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 3 cut(s) 46, 177, 406
BssNAI GTATAC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 281
Bst1107I GTATAC 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 504, 627
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 615
BstDEI CTNAG 5 cut(s) 93, 105, 334, 396, 417
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 322
BstKTI GATC 3 cut(s) 49, 180, 409
BstMBI GATC 3 cut(s) 46, 177, 406
BstMWI GCNNNNNNNGC 3 cut(s) 310, 319, 456
BstNI CCWGG 2 cut(s) 504, 627
BstNSI RCATGY 3 cut(s) 58, 613, 617
BstSCI CCNGG 3 cut(s) 502, 567, 625
BstSFI CTRYAG 3 cut(s) 70, 181, 308
BstV1I GCAGC 2 cut(s) 84, 322
BstV2I GAAGAC 2 cut(s) 421, 458
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BstZ17I GTATAC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 26, 110
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 2 cut(s) 315, 407
BtsIMutI CAGTG 3 cut(s) 179, 315, 407
Cac8I GCNNGC 1 cut(s) 615
CaiI CAGNNNCTG 1 cut(s) 307
CciI TCATGA 1 cut(s) 631
CfoI GCGC 2 cut(s) 77, 322
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 365
CviAII CATG 5 cut(s) 55, 112, 610, 614, 632
DdeI CTNAG 5 cut(s) 93, 105, 334, 396, 417
DpnI GATC 3 cut(s) 48, 179, 408
DpnII GATC 3 cut(s) 46, 177, 406
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 2 cut(s) 452, 535
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 221, 452
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoRI GAATTC 2 cut(s) 387, 421
EcoRII CCWGG 2 cut(s) 502, 625
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 2 cut(s) 452, 535
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 5 cut(s) 58, 115, 613, 617, 635
FaiI YATR 7 cut(s) 56, 60, 113, 224, 611, 615, 633
FaqI GGGAC 1 cut(s) 262
FatI CATG 5 cut(s) 54, 111, 609, 613, 631
FblI GTMKAC 2 cut(s) 59, 184
Fnu4HI GCNGC 3 cut(s) 73, 78, 311
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 2 cut(s) 452, 535
Fsp4HI GCNGC 3 cut(s) 73, 78, 311
FspBI CTAG 2 cut(s) 282, 549
FspI TGCGCA 1 cut(s) 321
GlaI GCGC 2 cut(s) 76, 321
GluI GCNGC 3 cut(s) 73, 78, 311
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 26, 110
HapII CCGG 2 cut(s) 141, 568
HhaI GCGC 2 cut(s) 77, 322
Hin1II CATG 5 cut(s) 58, 115, 613, 617, 635
Hin6I GCGC 2 cut(s) 75, 320
HinP1I GCGC 2 cut(s) 75, 320
HinfI GANTC 2 cut(s) 463, 635
HpaII CCGG 2 cut(s) 141, 568
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 60, 146, 185
Hpy188I TCNGA 2 cut(s) 240, 397
Hpy188III TCNNGA 4 cut(s) 204, 488, 522, 632
Hpy8I GTNNAC 3 cut(s) 60, 146, 185
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 76, 188
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 4 cut(s) 72, 310, 613, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 310, 319, 456
HpyF3I CTNAG 5 cut(s) 93, 105, 334, 396, 417
Hsp92II CATG 5 cut(s) 58, 115, 613, 617, 635
HspAI GCGC 2 cut(s) 75, 320
Kzo9I GATC 3 cut(s) 46, 177, 406
LmnI GCTCC 3 cut(s) 265, 506, 530
Lsp1109I GCAGC 2 cut(s) 84, 322
LweI GCATC 2 cut(s) 313, 562
MaeI CTAG 2 cut(s) 282, 549
MaeIII GTNAC 2 cut(s) 208, 346
MalI GATC 3 cut(s) 48, 179, 408
MboI GATC 3 cut(s) 46, 177, 406
MboII GAAGA 5 cut(s) 19, 22, 227, 421, 458
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 452, 535
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 5 cut(s) 82, 165, 229, 387, 421
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 2 cut(s) 472, 629
MmeI TCCRAC 3 cut(s) 228, 319, 392
MnlI CCTC 8 cut(s) 34, 44, 73, 98, 191, 256, 591, 610
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 615
MroXI GAANNNNTTC 1 cut(s) 391
MscI TGGCCA 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 2 cut(s) 141, 568
MspR9I CCNGG 3 cut(s) 504, 569, 627
MvaI CCWGG 2 cut(s) 504, 627
MwoI GCNNNNNNNGC 3 cut(s) 310, 319, 456
NciI CCSGG 1 cut(s) 569
NdeII GATC 3 cut(s) 46, 177, 406
NlaIII CATG 5 cut(s) 58, 115, 613, 617, 635
NlaIV GGNNCC 2 cut(s) 267, 532
NsbI TGCGCA 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 3 cut(s) 58, 613, 617
PaeI GCATGC 1 cut(s) 617
PagI TCATGA 1 cut(s) 631
PdmI GAANNNNTTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 3 cut(s) 74, 79, 312
PleI GAGTC 2 cut(s) 471, 629
PpsI GAGTC 2 cut(s) 471, 629
Psp6I CCWGG 2 cut(s) 502, 625
PspGI CCWGG 2 cut(s) 502, 625
PspN4I GGNNCC 2 cut(s) 267, 532
PspPI GGNCC 1 cut(s) 365
PstI CTGCAG 2 cut(s) 74, 312
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 177
SatI GCNGC 3 cut(s) 73, 78, 311
Sau3AI GATC 3 cut(s) 46, 177, 406
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 2 cut(s) 472, 629
ScrFI CCNGG 3 cut(s) 504, 569, 627
SduI GDGCHC 2 cut(s) 452, 535
SetI ASST 9 cut(s) 45, 65, 99, 287, 340, 550, 554, 602, 644
SfaNI GCATC 2 cut(s) 313, 562
SfcI CTRYAG 3 cut(s) 70, 181, 308
SinI GGWCC 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
SphI GCATGC 1 cut(s) 617
Sse9I AATT 5 cut(s) 82, 165, 229, 387, 421
SsiI CCGC 1 cut(s) 78
SspMI CTAG 2 cut(s) 282, 549
StyD4I CCNGG 3 cut(s) 502, 567, 625
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 217
TaqI TCGA 5 cut(s) 36, 49, 219, 446, 602
TaqII GACCGA 1 cut(s) 415
TasI AATT 5 cut(s) 82, 165, 229, 387, 421
TauI GCSGC 1 cut(s) 80
TscAI CASTG 3 cut(s) 179, 322, 407
TseI GCWGC 2 cut(s) 72, 310
TspDTI ATGAA 3 cut(s) 128, 348, 558
TspRI CASTG 3 cut(s) 179, 322, 407
VpaK11BI GGWCC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 3 cut(s) 165, 387, 421
XceI RCATGY 3 cut(s) 58, 613, 617
XmaJI CCTAGG 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 59, 184
XmnI GAANNNNTTC 1 cut(s) 391
XspI CTAG 2 cut(s) 282, 549
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.