MD05G1039400.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010037
Physical Location & Seq
Forward (+)
6635271 .. 6637866
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1039400.v1.1.491

Sequence Viewer

Length: 2541 bp
ATGGCGCTCCAAACCCACATCAGTCTTTCACACCTTCTCTTGATCGCCTTTCTCCTCTCCGCCGCCGTTTCCGCCGCTTCCGCCGCCACTACAATCTCACCAGGCTCATCTCTCTCCGCCGCCAACCCAAACACCGCCTGGTCCTCCCCAAACGACACCTTCTCCCTCGGCTTCATCTCCTCCGACCCCCCCACTTCACCGCCTTCTTACGTCGCCGCCATCTCCTACTCCGGCGGCGTCCCCGTCTGGTCCACTGGTGCAGGAAAAGTAGTTGACTCCGGTGGAACCCTCCAGTTCCTCCAATCCGGCACCCTTCGCCTCGTCAACGGCTCCGGTGCCACTCTCTGGGACTCCAACACCACCAGCCGCGGCGTCTCTTCGGCTGAGATTGACGATTCGGGCAACTTGGTACTCCGAAACGGCACTGTTACGGTCTGGTCCAGCTTCGACAACCCCACTGATTCGATCGTCCCCGGGCAGAACTTCACTGTGAGTAAGGTTTTGCGATCTGGGTTGTACTCGTTTAAGCTTGTGAAAAACGGGAACCTTACACTTTTGTGGAATGATATTAATGTGTATTGGAATCAGGGATTGAATTCTTCTGTTAATACGAATTTGAGTTCGCCTACTCTAGGATTGCGGTCAATTGGTATTCTGTCAATTTCTGATCCACAATTGTCCACTGATGCTATTGTTGCTTATAGTAGTGATTATGCCGAAGCGGGTGATATTTTGCGGTTTTTGAAGTTGGGGAGTGATGGGAATCTGAGGATTTATAGCTCTACTAGAGGTAGTGGGACTGTAACAGAGAGATGGGCAGCTGTTACTGATCAGTGTGAGGTTTTCGGGTATTGTGGGGACATGGGAGTTTGTAGTTATAACGATTCTGATCCGGTATGCGGATGTATGTCTCTTAATTTTGAGCTTGTTGATCCCAAGGATAGCAGGAAAGGGTGTAAGAGGAAGATGGAGATTAAGGATTGTCCTCAGAGTGTGACTATGTTGGACTTGGAACATACTCGGTTCTTGACGTACCCTCCTGAGACAGAGTCGCAGATTTTCTTTGCGGGAATATCGGCTTGCAGGTTGAATTGTCTTGGAAGTAGTGCTTGTGATGCTTCAACGTCTTTGTCTGATGGCACCGGGCTATGTTACTCCAAGACACCAGGTTTTCTGAGCGGGTATCACAGTCCAGCTATGTCCAGTAGTTCATATATCAAGGTATGTGGACCAGTGGTTCCAAATCCATCATCATCACTGGGGACTGCTGGCAACAAAAAAGATTGGAAGTTGCGTGCTTGGATAGTGGTTGTTGTAGTTATTGCCACCCTCTTTGGTTTGATGGCATTTGAAGGTGGTTTGTGGTGGTGGTTTTGCAGAAACAGTCGCAGCTTTGGTGGATTGTCTGCTCAATATGCGCTTCTTGAGTATGCTTCTGGTGCACCGGTTCAGTTCTCGTATAAGGAGCTCCAACGCTCAACGAAGGGATTCAAGGAGAAGCTTGGGGCAGGAGGATTTGGGACTGTTTATAAAGGCATTCTTGCTAATAGAACTGTTGTTGCAGTTAAGCAACTTGAGGGGATTGAGCAAGGGGAGAAACAGTTCAGGATGGAGGTTGCAACAATTAGTAGCACACACCATTTGAATCTGGTGAGGCTGATTGGTTTTTGCTCTGAGGGGCGCCATAGGCTTCTGGTATATGAGTTCATGAAAAATGGGTCCCTTGATAGTTTCCTTTTTGCAACAGAAGAGCAATCAGGAAAGTTGTTGAATTGGGAGTCTCGGTTCAACGTTGCCCTCGGCACTGCAAGGGGGATCACATACCTTCATGAGGAGTGCCGAGACTGCATTGTGCATTGTGATATAAAACCGGAGAACATTCTGTTAGATGAGAATTACAATGCCAAAGTGTCAGATTTTGGCCTCGCAAAGCTCGTTAATCCAAAGGACCATAGGTATCGAACCTTGACAAGTGTTAGAGGAACTAGAGGGTATTTGGCACCAGAATGGCTAGCAAATCTTCCAATAACTTCCAAATCTGATGTTTACAGTTACGGTATGGTTTTGCTAGAGATAGTGAGTGGGAGAAGGAATTTTGAAGTTTCTGAGGCGACAAATAGGAAGAAGTTGTCCTTATGGGCTTTTGAGGAATTTGAGAAGGGTAACATCAAGGGAATTGTCGATAGAAGGTTAGTCGACCAAGATGTTGATATGGATCAAGTGAAGAGAGCAATTCAGGTAACCTTCTGGTGCATACAGGAACAACCATCTCACAGGCCAATGATGGGAAAGGTTGTGCAGATGCTGGAAGGAATTACAGAAATTGAAAACCCCCCAGACCCAAGGGCTGTCATCGAAGGGTCTGCCAGTGGAACCAGCATGACTGTGAGCGGTAATATCAGCCCTTTATCCACTGCCGCAGACTCAGCCCCAGCTCCCTCCTCATTTTCATCGTTCCAAATTTCGGGAGTATCGCCTCTAACATCAGGGAGGAATACGGAGAAGGCAACTGCATCCCTTATACAGTCCGGCCCAAATTGA

Protein Analysis

847

Amino Acids

91.47

Weight (kDa)

6.31

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 167 2e-13 D-mannose binding lectin
S_locus_glycop PF00954 241 - 303 9.5e-06 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 497 - 769 1.2e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 498 - 767 7.8e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 879, 1530
Acc36I ACCTGC 1 cut(s) 1074
AccB1I GGYRCC 5 cut(s) 308, 335, 1139, 1680, 1999
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 2 cut(s) 1179, 2391
AccI GTMKAC 1 cut(s) 2196
AccII CGCG 1 cut(s) 369
AclI AACGTT 1 cut(s) 1791
AclWI GGATC 5 cut(s) 662, 884, 926, 1823, 2223
AcsI RAATTY 5 cut(s) 595, 613, 2092, 2150, 2460
AcyI GRCGYC 3 cut(s) 237, 372, 1681
AfaI GTAC 3 cut(s) 411, 518, 1034
AfiI CCNNNNNNNGG 6 cut(s) 345, 632, 899, 1831, 2285, 2464
AgeI ACCGGT 1 cut(s) 1444
AjnI CCWGG 3 cut(s) 100, 137, 1165
AleI CACNNNNGTG 1 cut(s) 556
AloI GAACNNNNNNTCC 6 cut(s) 1221, 1253, 1586, 1618, 1769, 1801
Alw21I GWGCWC 2 cut(s) 1444, 1470
Alw26I GTCTC 5 cut(s) 379, 915, 1037, 1785, 1836
Alw44I GTGCAC 1 cut(s) 1440
AlwI GGATC 5 cut(s) 662, 884, 926, 1823, 2223
AlwNI CAGNNNCTG 1 cut(s) 2305
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 3 cut(s) 1921, 2276, 2530
ApaLI GTGCAC 1 cut(s) 1440
ApeKI GCWGC 2 cut(s) 818, 1389
ApoI RAATTY 5 cut(s) 595, 613, 2092, 2150, 2460
AseI ATTAAT 1 cut(s) 570
AsiGI ACCGGT 1 cut(s) 1444
Asp700I GAANNNNTTC 2 cut(s) 1487, 1601
AspLEI GCGC 3 cut(s) 7, 1420, 1683
AspS9I GGNCC 7 cut(s) 141, 249, 438, 1229, 1719, 1948, 2531
AsuC2I CCSGG 3 cut(s) 474, 475, 1144
AsuHPI GGTGA 4 cut(s) 90, 189, 737, 1663
AsuNHI GCTAGC 1 cut(s) 2011
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 6 cut(s) 141, 249, 438, 1229, 1719, 1948
BaeGI GKGCMC 1 cut(s) 1444
BanI GGYRCC 5 cut(s) 308, 335, 1139, 1680, 1999
BanII GRGCYC 1 cut(s) 1470
Bbv12I GWGCWC 2 cut(s) 1444, 1470
BbvI GCAGC 2 cut(s) 830, 1401
BceAI ACGGC 3 cut(s) 50, 343, 436
BcgI CGANNNNNNTGC 2 cut(s) 2345, 2379
BciT130I CCWGG 3 cut(s) 102, 139, 1167
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 3 cut(s) 474, 475, 1144
BcoDI GTCTC 5 cut(s) 379, 915, 1037, 1785, 1836
BfaI CTAG 5 cut(s) 632, 786, 1986, 2012, 2069
BfoI RGCGCY 2 cut(s) 8, 1684
BfuAI ACCTGC 1 cut(s) 1074
Bme1390I CCNGG 6 cut(s) 102, 139, 474, 475, 1144, 1167
Bme18I GGWCC 6 cut(s) 141, 249, 438, 1229, 1719, 1948
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 7 cut(s) 141, 249, 438, 1229, 1719, 1948, 2531
BmrFI CCNGG 6 cut(s) 102, 139, 474, 475, 1144, 1167
BmrI ACTGGG 1 cut(s) 1268
BmsI GCATC 4 cut(s) 676, 1105, 2292, 2522
BmtI GCTAGC 1 cut(s) 2015
BmuI ACTGGG 1 cut(s) 1268
BpmI CTGGAG 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 1445, 1595
BpuMI CCSGG 3 cut(s) 474, 475, 1144
BsaBI GATNNNNATC 3 cut(s) 762, 888, 2214
BsaHI GRCGYC 3 cut(s) 237, 372, 1681
BsaJI CCNNGG 7 cut(s) 166, 367, 472, 473, 936, 1798, 2342
BsaWI WCCGGW 5 cut(s) 278, 332, 892, 1444, 1870
BsaXI ACNNNNNCTCC 8 cut(s) 146, 176, 1021, 1051, 1586, 1616, 1769, 1799
Bsc4I CCNNNNNNNGG 6 cut(s) 345, 632, 899, 1831, 2285, 2464
Bse118I RCCGGY 1 cut(s) 1444
Bse1I ACTGG 6 cut(s) 259, 292, 1203, 1232, 1263, 2367
Bse8I GATNNNNATC 3 cut(s) 762, 888, 2214
BseBI CCWGG 3 cut(s) 102, 139, 1167
BseDI CCNNGG 7 cut(s) 166, 367, 472, 473, 936, 1798, 2342
BseGI GGATG 3 cut(s) 908, 1614, 2513
BseJI GATNNNNATC 3 cut(s) 762, 888, 2214
BseLI CCNNNNNNNGG 6 cut(s) 345, 632, 899, 1831, 2285, 2464
BseMII CTCAG 8 cut(s) 375, 758, 1001, 1032, 1166, 1665, 2097, 2439
BseNI ACTGG 6 cut(s) 259, 292, 1203, 1232, 1263, 2367
BseRI GAGGAG 4 cut(s) 44, 169, 1847, 2431
BseSI GKGCMC 1 cut(s) 1444
BseXI GCAGC 2 cut(s) 830, 1401
BseYI CCCAGC 1 cut(s) 2431
BsgI GTGCAG 2 cut(s) 279, 2318
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 468
BshFI GGCC 3 cut(s) 1923, 2278, 2532
BshNI GGYRCC 5 cut(s) 308, 335, 1139, 1680, 1999
BshTI ACCGGT 1 cut(s) 1444
BsiEI CGRYCG 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 1444, 1470
BsiHKCI CYCGRG 1 cut(s) 473
BslFI GGGAC 8 cut(s) 224, 362, 455, 811, 872, 1276, 1534, 1705
BslI CCNNNNNNNGG 6 cut(s) 345, 632, 899, 1831, 2285, 2464
BsmAI GTCTC 5 cut(s) 379, 915, 1037, 1785, 1836
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 8 cut(s) 224, 362, 455, 811, 872, 1276, 1534, 1705
BsmI GAATGC 1 cut(s) 1536
BsnI GGCC 3 cut(s) 1923, 2278, 2532
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 1444, 1470
Bsp143I GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
BspANI GGCC 3 cut(s) 1923, 2278, 2532
BspCNI CTCAG 8 cut(s) 376, 759, 1000, 1033, 1167, 1666, 2098, 2438
BspFNI CGCG 1 cut(s) 369
BspHI TCATGA 2 cut(s) 1707, 1828
BspMI ACCTGC 1 cut(s) 1074
BspOI GCTAGC 1 cut(s) 2015
BspPI GGATC 5 cut(s) 662, 884, 926, 1823, 2223
BspQI GCTCTTC 1 cut(s) 1743
BspT107I GGYRCC 5 cut(s) 308, 335, 1139, 1680, 1999
BsrBI CCGCTC 2 cut(s) 1179, 2391
BsrFI RCCGGY 1 cut(s) 1444
BsrI ACTGG 6 cut(s) 259, 292, 1203, 1232, 1263, 2367
BssAI RCCGGY 1 cut(s) 1444
BssECI CCNNGG 7 cut(s) 166, 367, 472, 473, 936, 1798, 2342
BssMI GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
BssNI GRCGYC 3 cut(s) 237, 372, 1681
BssT1I CCWWGG 2 cut(s) 936, 2342
Bst2UI CCWGG 3 cut(s) 102, 139, 1167
Bst6I CTCTTC 3 cut(s) 382, 1743, 2219
BstACI GRCGYC 3 cut(s) 237, 372, 1681
BstC8I GCNNGC 4 cut(s) 1081, 1270, 1296, 2013
BstDEI CTNAG 8 cut(s) 384, 767, 987, 1041, 1175, 1674, 2106, 2425
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 2239
BstENI CCTNNNNNAGG 2 cut(s) 630, 1829
BstF5I GGATG 3 cut(s) 908, 1614, 2513
BstFNI CGCG 1 cut(s) 369
BstH2I RGCGCY 2 cut(s) 8, 1684
BstHHI GCGC 3 cut(s) 7, 1420, 1683
BstKTI GATC 9 cut(s) 45, 468, 509, 670, 832, 892, 934, 1818, 2218
BstMAI GTCTC 5 cut(s) 379, 915, 1037, 1785, 1836
BstMBI GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
BstMCI CGRYCG 1 cut(s) 468
BstNI CCWGG 3 cut(s) 102, 139, 1167
BstPI GGTNACC 1 cut(s) 2239
BstSCI CCNGG 6 cut(s) 100, 137, 472, 473, 1142, 1165
BstSLI GKGCMC 1 cut(s) 1444
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 830, 1401
BsuRI GGCC 3 cut(s) 1923, 2278, 2532
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 908, 1614, 2513
BtsI GCAGTG 2 cut(s) 1803, 2412
BveI ACCTGC 1 cut(s) 1074
Cac8I GCNNGC 4 cut(s) 1081, 1270, 1296, 2013
CaiI CAGNNNCTG 1 cut(s) 2305
CciI TCATGA 2 cut(s) 1707, 1828
CfoI GCGC 3 cut(s) 7, 1420, 1683
Cfr10I RCCGGY 1 cut(s) 1444
Cfr13I GGNCC 7 cut(s) 141, 249, 438, 1229, 1719, 1948, 2531
Cfr42I CCGCGG 1 cut(s) 370
Cfr9I CCCGGG 1 cut(s) 473
CseI GACGC 2 cut(s) 226, 361
CsiI ACCWGGT 1 cut(s) 1165
Csp6I GTAC 3 cut(s) 410, 517, 1033
CspAI ACCGGT 1 cut(s) 1444
CspCI CAANNNNNGTGG 4 cut(s) 1207, 1242, 1315, 1350
CviAII CATG 4 cut(s) 862, 1708, 1829, 2380
CviQI GTAC 3 cut(s) 410, 517, 1033
DdeI CTNAG 8 cut(s) 384, 767, 987, 1041, 1175, 1674, 2106, 2425
DinI GGCGCC 1 cut(s) 1682
DpnI GATC 9 cut(s) 44, 467, 508, 669, 831, 891, 933, 1817, 2217
DpnII GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
Eam1104I CTCTTC 3 cut(s) 382, 1743, 2219
EarI CTCTTC 3 cut(s) 382, 1743, 2219
EciI GGCGGA 4 cut(s) 49, 61, 70, 106
Ecl136II GAGCTC 1 cut(s) 1468
Eco130I CCWWGG 2 cut(s) 936, 2342
Eco24I GRGCYC 1 cut(s) 1470
Eco47I GGWCC 6 cut(s) 141, 249, 438, 1229, 1719, 1948
Eco53kI GAGCTC 1 cut(s) 1468
Eco88I CYCGRG 1 cut(s) 473
Eco91I GGTNACC 1 cut(s) 2239
EcoICRI GAGCTC 1 cut(s) 1468
EcoNI CCTNNNNNAGG 2 cut(s) 630, 1829
EcoO109I RGGNCCY 1 cut(s) 1719
EcoO65I GGTNACC 1 cut(s) 2239
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 3 cut(s) 100, 137, 1165
EcoT14I CCWWGG 2 cut(s) 936, 2342
EcoT38I GRGCYC 1 cut(s) 1470
EgeI GGCGCC 1 cut(s) 1682
EheI GGCGCC 1 cut(s) 1682
ErhI CCWWGG 2 cut(s) 936, 2342
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 4 cut(s) 865, 1711, 1832, 2383
FalI AAGNNNNNCTT 6 cut(s) 1093, 1125, 1524, 1556, 2117, 2149
FaqI GGGAC 8 cut(s) 224, 362, 455, 811, 872, 1276, 1534, 1705
FatI CATG 4 cut(s) 861, 1707, 1828, 2379
FauI CCCGC 3 cut(s) 715, 1060, 1172
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 1 cut(s) 2196
FokI GGATG 3 cut(s) 915, 1621, 2500
FriOI GRGCYC 1 cut(s) 1470
FspBI CTAG 5 cut(s) 632, 786, 1986, 2012, 2069
GlaI GCGC 3 cut(s) 6, 1419, 1682
GsaI CCCAGC 1 cut(s) 2435
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 8, 1684
HaeIII GGCC 3 cut(s) 1923, 2278, 2532
HgaI GACGC 2 cut(s) 226, 361
HhaI GCGC 3 cut(s) 7, 1420, 1683
Hin1I GRCGYC 3 cut(s) 237, 372, 1681
Hin1II CATG 4 cut(s) 865, 1711, 1832, 2383
Hin6I GCGC 3 cut(s) 5, 1418, 1681
HinP1I GCGC 3 cut(s) 5, 1418, 1681
HincII GTYRAC 3 cut(s) 274, 325, 2197
HindII GTYRAC 3 cut(s) 274, 325, 2197
HindIII AAGCTT 2 cut(s) 527, 1499
HphI GGTGA 4 cut(s) 90, 189, 737, 1663
Hpy166II GTNNAC 8 cut(s) 252, 274, 325, 681, 1229, 1442, 2047, 2197
Hpy188III TCNNGA 9 cut(s) 40, 1027, 1040, 1424, 1606, 1708, 1758, 1829, 2466
Hpy8I GTNNAC 8 cut(s) 252, 274, 325, 681, 1229, 1442, 2047, 2197
Hpy99I CGWCG 1 cut(s) 215
HpyCH4IV ACGT 4 cut(s) 210, 1031, 1124, 1791
HpyF3I CTNAG 8 cut(s) 384, 767, 987, 1041, 1175, 1674, 2106, 2425
HpySE526I ACGT 4 cut(s) 210, 1031, 1124, 1791
Hsp92I GRCGYC 3 cut(s) 237, 372, 1681
Hsp92II CATG 4 cut(s) 865, 1711, 1832, 2383
HspAI GCGC 3 cut(s) 5, 1418, 1681
KasI GGCGCC 1 cut(s) 1680
KflI GGGWCCC 1 cut(s) 1719
Ksp22I TGATCA 1 cut(s) 829
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
LguI GCTCTTC 1 cut(s) 1743
LmnI GCTCC 5 cut(s) 12, 335, 1465, 1473, 2440
Lsp1109I GCAGC 2 cut(s) 830, 1401
LweI GCATC 4 cut(s) 676, 1105, 2292, 2522
MabI ACCWGGT 1 cut(s) 1165
MaeI CTAG 5 cut(s) 632, 786, 1986, 2012, 2069
MaeII ACGT 4 cut(s) 210, 1031, 1124, 1791
MaeIII GTNAC 8 cut(s) 427, 802, 823, 994, 1151, 2051, 2162, 2239
MalI GATC 9 cut(s) 44, 467, 508, 669, 831, 891, 933, 1817, 2217
MbiI CCGCTC 2 cut(s) 1179, 2391
MboI GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
MboII GAAGA 7 cut(s) 369, 591, 976, 1760, 2012, 2134, 2236
MfeI CAATTG 2 cut(s) 645, 674
MhlI GDGCHC 2 cut(s) 1444, 1470
Mly113I GGCGCC 1 cut(s) 1681
MlyI GAGTC 5 cut(s) 269, 344, 1058, 1787, 2417
MmeI TCCRAC 4 cut(s) 207, 378, 984, 1495
MroXI GAANNNNTTC 2 cut(s) 1487, 1601
MseI TTAA 7 cut(s) 525, 570, 606, 915, 975, 1566, 1938
MslI CAYNNNNRTG 3 cut(s) 556, 2005, 2384
MspA1I CMGCKG 2 cut(s) 369, 821
MspR9I CCNGG 6 cut(s) 102, 139, 474, 475, 1144, 1167
MunI CAATTG 2 cut(s) 645, 674
Mva1269I GAATGC 1 cut(s) 1536
MvaI CCWGG 3 cut(s) 102, 139, 1167
MvnI CGCG 1 cut(s) 369
NarI GGCGCC 1 cut(s) 1681
NciI CCSGG 3 cut(s) 474, 475, 1144
NdeII GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
NheI GCTAGC 1 cut(s) 2011
NlaIII CATG 4 cut(s) 865, 1711, 1832, 2383
NmeAIII GCCGAG 3 cut(s) 147, 1779, 1865
NmuCI GTSAC 1 cut(s) 994
OliI CACNNNNGTG 1 cut(s) 556
PagI TCATGA 2 cut(s) 1707, 1828
PciSI GCTCTTC 1 cut(s) 1743
PcsI WCGNNNNNNNCGW 1 cut(s) 1932
PctI GAATGC 1 cut(s) 1536
PdmI GAANNNNTTC 2 cut(s) 1487, 1601
PfeI GAWTC 7 cut(s) 395, 461, 583, 763, 884, 1488, 1645
PflFI GACNNNGTC 1 cut(s) 1048
PflMI CCANNNNNTGG 1 cut(s) 345
PinAI ACCGGT 1 cut(s) 1444
Ple19I CGATCG 1 cut(s) 468
PleI GAGTC 5 cut(s) 269, 344, 1057, 1786, 2417
PluTI GGCGCC 1 cut(s) 1684
PpsI GAGTC 5 cut(s) 269, 344, 1057, 1786, 2417
PpuMI RGGWCCY 1 cut(s) 1719
PshBI ATTAAT 1 cut(s) 570
PsiI TTATAA 2 cut(s) 879, 1530
Psp124BI GAGCTC 1 cut(s) 1470
Psp1406I AACGTT 1 cut(s) 1791
Psp5II RGGWCCY 1 cut(s) 1719
Psp6I CCWGG 3 cut(s) 100, 137, 1165
PspEI GGTNACC 1 cut(s) 2239
PspFI CCCAGC 1 cut(s) 2431
PspGI CCWGG 3 cut(s) 100, 137, 1165
PspPI GGNCC 7 cut(s) 141, 249, 438, 1229, 1719, 1948, 2531
PspPPI RGGWCCY 1 cut(s) 1719
PstNI CAGNNNCTG 1 cut(s) 2305
PsyI GACNNNGTC 1 cut(s) 1048
PvuI CGATCG 1 cut(s) 468
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 3 cut(s) 411, 518, 1034
RsaNI GTAC 3 cut(s) 410, 517, 1033
RseI CAYNNNNRTG 3 cut(s) 556, 2005, 2384
SacI GAGCTC 1 cut(s) 1470
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 2195
SapI GCTCTTC 1 cut(s) 1743
SaqAI TTAA 7 cut(s) 525, 570, 606, 915, 975, 1566, 1938
Sau3AI GATC 9 cut(s) 42, 465, 506, 667, 829, 889, 931, 1815, 2215
Sau96I GGNCC 7 cut(s) 141, 249, 438, 1229, 1719, 1948, 2531
SchI GAGTC 5 cut(s) 269, 344, 1058, 1787, 2417
ScrFI CCNGG 6 cut(s) 102, 139, 474, 475, 1144, 1167
SduI GDGCHC 2 cut(s) 1444, 1470
SexAI ACCWGGT 1 cut(s) 1165
SfaNI GCATC 4 cut(s) 676, 1105, 2292, 2522
SfoI GGCGCC 1 cut(s) 1682
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SinI GGWCC 6 cut(s) 141, 249, 438, 1229, 1719, 1948
SmaI CCCGGG 1 cut(s) 475
SmiMI CAYNNNNRTG 3 cut(s) 556, 2005, 2384
SmlI CTYRAG 2 cut(s) 1424, 1574
SmoI CTYRAG 2 cut(s) 1424, 1574
SspDI GGCGCC 1 cut(s) 1680
SspMI CTAG 5 cut(s) 632, 786, 1986, 2012, 2069
SstI GAGCTC 1 cut(s) 1470
StyD4I CCNGG 6 cut(s) 100, 137, 472, 473, 1142, 1165
StyI CCWWGG 2 cut(s) 936, 2342
TaiI ACGT 4 cut(s) 213, 1034, 1127, 1794
TaqI TCGA 6 cut(s) 447, 464, 1960, 2181, 2196, 2355
TatI WGTACW 1 cut(s) 516
TauI GCSGC 9 cut(s) 65, 77, 86, 122, 218, 237, 369, 372, 2420
TfiI GAWTC 7 cut(s) 395, 461, 583, 763, 884, 1488, 1645
Tru1I TTAA 7 cut(s) 525, 570, 606, 915, 975, 1566, 1938
Tru9I TTAA 7 cut(s) 525, 570, 606, 915, 975, 1566, 1938
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 2 cut(s) 818, 1389
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 6 cut(s) 163, 1200, 1696, 1724, 1817, 2439
TspGWI ACGGA 1 cut(s) 2513
TspMI CCCGGG 1 cut(s) 473
Tth111I GACNNNGTC 1 cut(s) 1048
Van91I CCANNNNNTGG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 1440
VpaK11BI GGWCC 6 cut(s) 141, 249, 438, 1229, 1719, 1948
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 2 cut(s) 630, 1829
XapI RAATTY 5 cut(s) 595, 613, 2092, 2150, 2460
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
XmaI CCCGGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 2196
XmnI GAANNNNTTC 2 cut(s) 1487, 1601
XspI CTAG 5 cut(s) 632, 786, 1986, 2012, 2069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.