Rw6G016020

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010037
Physical Location & Seq
Reverse (-)
31175579 .. 31178544
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016020.1

Sequence Viewer

Length: 2499 bp
ATGCCGATCAAATTCCGGCTTGCGCTCCTCCCTCTCTTCTGTCTTTTATCCCAAACCATCTGGGCCGCCACAATTTCACCCGGCTCTTCTCTCCTTGCTTCTAATCCTAACCAAACCTGGTCTTCCCCAGACAGCTCCTTCTCTTTCGGCTTCATTCCCTCTGACCCACCCACCTCACCCCCTTCTTTCACCGCCGCCATAACCTACTCCGGCGGCGTCCCCATCTGGTCTCCCGGCCGCAGCGTTGACTCCGCCGGGGCCCTCCACTTCCTCTCATCCGGCGCCCTCCGCCTCGTCGACGGCTCCAACAGAACCATATGGGACTCCGACACCGCCAGCCGCGGCGTCTCCTCCGCTGAGCTCGACGGTTCCGGCAACCTGGTGCTCCGGAACGGCACCGGTGCTGCGGTCTGGTCCAGCTTCGACAACCCGACGGATACGATCGTGCCGTCGCAGAACTTCACTGTGGGTAAGGTCTTGCGATCTGGTATGTACTCGTTTAAGCTGGTCAAGAATGGGAATCTGACTCTGTTGTGGAACGATAGTATTGTGTATTGGAATCAAGGGTTGAACTCGTCTGTTACTAACAACACTCCCAATTTGACTTCACCCACTTTAGGATTGCAGTCTATTGGTATTCTGAGCATTTCTGATCCAAAGTTGCCCACTGCTGCTATTGTTGCTTATAGTAATGATTATGCTGAGGCTGGTGATATTTTGAGGTTCTTGAAGCTAGAGAGTGATGGGAATGTGAGGATTTATAGCTCTAGTAAAGGTAGCGGGGATAAAACTGAGAGATGGGCAGCTGTTACTGATCAGTGTCAGGTGTTTGGCTACTGTGGGAACATGGGAATTTGCAGTTATAATGATTCCAATCCCATATGCAGCTGCCCTTCTCTTAATTTCGAGCCAGTTGATACCAAAGATAGCAGGAAAGGGTGTAGGAGGAAGATGGAGATTAAGGACTGCCCTCAGAGTGTGACTATGTTGGATTTGGTTCATACTAGGTTCTTGACCTACCCTCCTGAGACGGATTCACAGGTTTTCTTTGTGGGTATATCAGCCTGTAGGTTAAACTGTCTTGTTAATGATCCTTGTGATGCTTCAACATCTTTGTCTGATGGCACAGGGCTCTGTTACTACAAGACACCCGGTTTTCTCAGCGGGTATCACACTCCAGCTTTGACTAGCAGTTCATATATCAAGGTTTGTGGACCGGTGATTCCAAATCCGCCCTCTTCGTTGGATAGTGCTGGCAAGAAAAAGGATTGGAAGATCAGGGCTTGGATAGTGGCTCTTGTGGTTGTCGCTTCCCTTTTGGGTTTGATGGCACTGGAGGGTGGTCTGTGGTGGTGGTTTTGCAGGAACAGTCCAAGCTTTGGTGCATTGTCTGCTCAGTATGCTCTTCTTGAGTATGCTTCTGGTGCGCCAGTTCAGTTTTCGTACAAAGAACTCCAGCGCTCTACCAAGGGATTCAAGGAGAAGCTTGGAGCCGGAGGATTTGGAGCTGTTTATAAGGGCATTCTTGCTAATAGAACTGTTGTTGCAGTGAAGCAACTCGAGGGGATTGAGCAAGGAGAAAAGCAGTTTAGGATGGAGGTTGCAACAATTAGTAGCACCCATCATTTAAATCTAGTGAGGCTGATTGGTTTCTGCTCTGAGGGCCGTCATAGGCTTTTAGTATATGAGTTCATGAAAAATGGGTCCCTTGATGATTTCCTTTTTGCCACAGAAGAGCAGTCAGGAAAATTCTTAAGTTGGGAGAATCGATTCAAGATTGCCCTTGGCACTGCAAGGGGGATCACATACCTTCACGAGGAGTGCCGAGACTGCATTGTGCATTGTGATATAAAACCAGAGAACATTCTCTTGGATGAGAATTACAATTCCAAAGTATCTGATTTTGGCCTCGCTAAGCTTGTTAGTCCAAAGGACCACAGGTACCGAACTTTGACCAGTGTTAGAGGGACTAGAGGTTATTTGGCACCAGAATGGTTGGCAAATCTGCCTATAACTTCCAAATCTGATATTTATAGTTATGGTATGGTTCTGTTAGAGATAGTTAGTGGGAGAAGGAATTTTGAAGTTTCTGAGGAGACAGATAGGAAAAAGTTCTCCATATGGGCCTTTGACGAATTTGAGAAGGGTAATATCAAGGGAATCGTTGATAAAAGGCTGGCTGACCAAGATGTTGATATGGATCAAGTGATGAGAGCAATTCAGGTGACATTCTGGTGCATACAAGAGCAGCCATCTCATAGGCCAATGATGGGAAAAGTTGTACAAATGCTGGAAGGAATTACAGAGATGGGGAAACCCCCATCTCCAAGGGCTATCATTGAAGGGCCTATAATTGAAAGGCCTGTTAGTGGAACCAGCACTAGTGTTGTAGCCCCCTCCTCATTTTCATCATTTCAAATTTCAGAAGTATCGCCTTCTGCACCAGCTAGGAACATGGAGACAGCGACTGCATCCCTTATACAGTCAGACCTGAGTTGA

Protein Analysis

832

Amino Acids

91.03

Weight (kDa)

6.59

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 83 - 158 3.3e-13 D-mannose binding lectin
S_locus_glycop PF00954 237 - 298 1.1e-06 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 492 - 764 9e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 493 - 762 2.4e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 864, 1515
Acc65I GGTACC 1 cut(s) 1941
AccB1I GGYRCC 4 cut(s) 281, 395, 1941, 1984
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 387
AclWI GGATC 4 cut(s) 647, 1085, 1808, 2208
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 6 cut(s) 11, 852, 1748, 2077, 2135, 2418
AcyI GRCGYC 3 cut(s) 216, 282, 345
AfaI GTAC 4 cut(s) 494, 1445, 1943, 2283
AfeI AGCGCT 1 cut(s) 1460
AfiI CCNNNNNNNGG 5 cut(s) 617, 1379, 1816, 2270, 2369
AflII CTTAAG 1 cut(s) 1753
AgeI ACCGGT 2 cut(s) 398, 1216
AgsI TTSAA 9 cut(s) 571, 730, 1107, 1477, 1774, 2084, 2342, 2357, 2417
AhlI ACTAGT 1 cut(s) 2381
AjnI CCWGG 2 cut(s) 116, 378
AjuI GAANNNNNNNTTGG 2 cut(s) 1853, 1885
Alw21I GWGCWC 2 cut(s) 363, 387
Alw26I GTCTC 6 cut(s) 234, 352, 1022, 1821, 2090, 2453
AlwI GGATC 4 cut(s) 647, 1085, 1808, 2208
AlwNI CAGNNNCTG 1 cut(s) 2468
Ama87I CYCGRG 1 cut(s) 1559
Aor13HI TCCGGA 1 cut(s) 387
Aor51HI AGCGCT 1 cut(s) 1460
AoxI GGCC 9 cut(s) 63, 235, 258, 1663, 1906, 2124, 2261, 2345, 2360
ApaI GGGCCC 1 cut(s) 262
ApeKI GCWGC 7 cut(s) 240, 404, 671, 803, 885, 888, 2248
ApoI RAATTY 6 cut(s) 11, 852, 1748, 2077, 2135, 2418
AsiGI ACCGGT 2 cut(s) 398, 1216
Asp700I GAANNNNTTC 2 cut(s) 1769, 2111
Asp718I GGTACC 1 cut(s) 1941
AspLEI GCGC 4 cut(s) 25, 284, 1429, 1461
AsuC2I CCSGG 4 cut(s) 81, 234, 256, 1152
AsuHPI GGTGA 7 cut(s) 69, 168, 181, 600, 722, 1231, 2236
AvaI CYCGRG 1 cut(s) 1559
AvaII GGWCC 4 cut(s) 414, 1214, 1704, 1933
BaeGI GKGCMC 1 cut(s) 262
BaeI ACNNNNGTAYC 2 cut(s) 429, 462
BanI GGYRCC 4 cut(s) 281, 395, 1941, 1984
BanII GRGCYC 3 cut(s) 262, 363, 1134
BauI CACGAG 1 cut(s) 1814
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 363, 387
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 7 cut(s) 252, 391, 658, 815, 875, 897, 2260
BceAI ACGGC 4 cut(s) 316, 409, 433, 1650
BciT130I CCWGG 2 cut(s) 118, 380
BciVI GTATCC 1 cut(s) 430
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 4 cut(s) 81, 234, 256, 1152
BcoDI GTCTC 6 cut(s) 234, 352, 1022, 1821, 2090, 2453
BcuI ACTAGT 1 cut(s) 2381
BfaI CTAG 8 cut(s) 734, 768, 1005, 1188, 1634, 1971, 2382, 2448
BfmI CTRYAG 1 cut(s) 1066
BfoI RGCGCY 2 cut(s) 285, 1462
BfrI CTTAAG 1 cut(s) 1753
BfuI GTATCC 1 cut(s) 430
BlpI GCTNAGC 2 cut(s) 357, 1914
Bme1390I CCNGG 6 cut(s) 81, 118, 234, 256, 380, 1152
Bme18I GGWCC 4 cut(s) 414, 1214, 1704, 1933
BmeT110I CYCGRG 1 cut(s) 1559
BmrFI CCNGG 6 cut(s) 81, 118, 234, 256, 380, 1152
BmsI GCATC 2 cut(s) 1090, 2480
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 3 cut(s) 1161, 1355, 1439
Bpu10I CCTNAGC 1 cut(s) 702
Bpu1102I GCTNAGC 2 cut(s) 357, 1914
BpuEI CTTGAG 1 cut(s) 1430
BpuMI CCSGG 4 cut(s) 81, 234, 256, 1152
Bsa29I ATCGAT 1 cut(s) 1768
BsaBI GATNNNNATC 2 cut(s) 873, 2199
BsaHI GRCGYC 3 cut(s) 216, 282, 345
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 5 cut(s) 255, 340, 1467, 1783, 2327
BsaWI WCCGGW 3 cut(s) 387, 398, 1216
BsaXI ACNNNNNCTCC 2 cut(s) 1006, 1036
Bsc4I CCNNNNNNNGG 5 cut(s) 617, 1379, 1816, 2270, 2369
Bse118I RCCGGY 2 cut(s) 398, 1216
Bse1I ACTGG 4 cut(s) 911, 1338, 1430, 1956
Bse8I GATNNNNATC 2 cut(s) 873, 2199
BseAI TCCGGA 1 cut(s) 387
BseBI CCWGG 2 cut(s) 118, 380
BseCI ATCGAT 1 cut(s) 1768
BseDI CCNNGG 5 cut(s) 255, 340, 1467, 1783, 2327
BseGI GGATG 4 cut(s) 275, 1599, 1879, 2471
BseJI GATNNNNATC 2 cut(s) 873, 2199
BseLI CCNNNNNNNGG 5 cut(s) 617, 1379, 1816, 2270, 2369
BseNI ACTGG 4 cut(s) 911, 1338, 1430, 1956
BseRI GAGGAG 5 cut(s) 17, 340, 1832, 2108, 2389
BseSI GKGCMC 1 cut(s) 262
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 7 cut(s) 252, 391, 658, 815, 875, 897, 2260
BsgI GTGCAG 1 cut(s) 2424
Bsh1236I CGCG 1 cut(s) 342
Bsh1285I CGRYCG 2 cut(s) 238, 444
BshFI GGCC 9 cut(s) 65, 237, 260, 1665, 1908, 2126, 2263, 2347, 2362
BshNI GGYRCC 4 cut(s) 281, 395, 1941, 1984
BshTI ACCGGT 2 cut(s) 398, 1216
BshVI ATCGAT 1 cut(s) 1768
BsiEI CGRYCG 2 cut(s) 238, 444
BsiHKAI GWGCWC 2 cut(s) 363, 387
BsiHKCI CYCGRG 1 cut(s) 1559
BslFI GGGAC 4 cut(s) 203, 335, 1690, 1981
BslI CCNNNNNNNGG 5 cut(s) 617, 1379, 1816, 2270, 2369
BsmAI GTCTC 6 cut(s) 234, 352, 1022, 1821, 2090, 2453
BsmBI CGTCTC 2 cut(s) 352, 1022
BsmFI GGGAC 4 cut(s) 203, 335, 1690, 1981
BsmI GAATGC 1 cut(s) 1521
BsnI GGCC 9 cut(s) 65, 237, 260, 1665, 1908, 2126, 2263, 2347, 2362
Bso31I GGTCTC 1 cut(s) 234
BsoBI CYCGRG 1 cut(s) 1559
Bsp120I GGGCCC 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 262, 363, 387, 1134
Bsp13I TCCGGA 1 cut(s) 387
Bsp1407I TGTACA 1 cut(s) 2281
Bsp143I GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
Bsp1720I GCTNAGC 2 cut(s) 357, 1914
BspANI GGCC 9 cut(s) 65, 237, 260, 1665, 1908, 2126, 2263, 2347, 2362
BspDI ATCGAT 1 cut(s) 1768
BspEI TCCGGA 1 cut(s) 387
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 1692
BspPI GGATC 4 cut(s) 647, 1085, 1808, 2208
BspQI GCTCTTC 3 cut(s) 91, 1410, 1728
BspT107I GGYRCC 4 cut(s) 281, 395, 1941, 1984
BspTI CTTAAG 1 cut(s) 1753
BspTNI GGTCTC 1 cut(s) 234
BsrFI RCCGGY 2 cut(s) 398, 1216
BsrGI TGTACA 1 cut(s) 2281
BsrI ACTGG 4 cut(s) 911, 1338, 1430, 1956
BssAI RCCGGY 2 cut(s) 398, 1216
BssECI CCNNGG 5 cut(s) 255, 340, 1467, 1783, 2327
BssMI GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
BssNI GRCGYC 3 cut(s) 216, 282, 345
BssSI CACGAG 1 cut(s) 1814
BssT1I CCWWGG 3 cut(s) 1467, 1783, 2327
Bst2BI CACGAG 1 cut(s) 1814
Bst2UI CCWGG 2 cut(s) 118, 380
Bst4CI ACNGT 7 cut(s) 368, 466, 839, 1079, 1370, 1540, 2484
Bst6I CTCTTC 5 cut(s) 41, 91, 1243, 1410, 1728
BstACI GRCGYC 3 cut(s) 216, 282, 345
BstAFI CTTAAG 1 cut(s) 1753
BstAPI GCANNNNNTGC 1 cut(s) 1391
BstAUI TGTACA 1 cut(s) 2281
BstC8I GCNNGC 4 cut(s) 21, 337, 1255, 2178
BstDSI CCRYGG 1 cut(s) 340
BstENI CCTNNNNNAGG 1 cut(s) 1814
BstF5I GGATG 4 cut(s) 275, 1599, 1879, 2471
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 2 cut(s) 285, 1462
BstHHI GCGC 4 cut(s) 25, 284, 1429, 1461
BstKTI GATC 9 cut(s) 9, 444, 485, 655, 817, 1093, 1278, 1803, 2203
BstMAI GTCTC 6 cut(s) 234, 352, 1022, 1821, 2090, 2453
BstMBI GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
BstMCI CGRYCG 2 cut(s) 238, 444
BstMWI GCNNNNNNNGC 7 cut(s) 288, 680, 1391, 1400, 1424, 1662, 1830
BstNI CCWGG 2 cut(s) 118, 380
BstSCI CCNGG 6 cut(s) 79, 116, 232, 254, 378, 1150
BstSFI CTRYAG 1 cut(s) 1066
BstSLI GKGCMC 1 cut(s) 262
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 7 cut(s) 252, 391, 658, 815, 875, 897, 2260
BstV2I GAAGAC 1 cut(s) 114
BstZI CGGCCG 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 1768
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 9 cut(s) 65, 237, 260, 1665, 1908, 2126, 2263, 2347, 2362
BsuTUI ATCGAT 1 cut(s) 1768
BtgI CCRYGG 1 cut(s) 340
BtsCI GGATG 4 cut(s) 275, 1599, 1879, 2471
BtsI GCAGTG 3 cut(s) 666, 1554, 1788
BtsIMutI CAGTG 7 cut(s) 462, 666, 824, 1331, 1554, 1788, 1963
Cac8I GCNNGC 4 cut(s) 21, 337, 1255, 2178
CaiI CAGNNNCTG 1 cut(s) 2468
CciI TCATGA 1 cut(s) 1692
CfoI GCGC 4 cut(s) 25, 284, 1429, 1461
Cfr10I RCCGGY 2 cut(s) 398, 1216
Cfr42I CCGCGG 1 cut(s) 343
ClaI ATCGAT 1 cut(s) 1768
CseI GACGC 2 cut(s) 205, 334
CsiI ACCWGGT 2 cut(s) 116, 378
Csp6I GTAC 4 cut(s) 493, 1444, 1942, 2282
CspAI ACCGGT 2 cut(s) 398, 1216
CspCI CAANNNNNGTGG 2 cut(s) 1192, 1227
CviAII CATG 3 cut(s) 847, 1693, 2455
CviQI GTAC 4 cut(s) 493, 1444, 1942, 2282
DinI GGCGCC 1 cut(s) 283
DpnI GATC 9 cut(s) 8, 443, 484, 654, 816, 1092, 1277, 1802, 2202
DpnII GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
DraI TTTAAA 1 cut(s) 1629
EaeI YGGCCR 1 cut(s) 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 5 cut(s) 41, 91, 1243, 1410, 1728
EarI CTCTTC 5 cut(s) 41, 91, 1243, 1410, 1728
EciI GGCGGA 3 cut(s) 241, 278, 1221
Ecl136II GAGCTC 1 cut(s) 361
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 1467, 1783, 2327
Eco147I AGGCCT 1 cut(s) 2362
Eco24I GRGCYC 3 cut(s) 262, 363, 1134
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 414, 1214, 1704, 1933
Eco47III AGCGCT 1 cut(s) 1460
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 361
Eco88I CYCGRG 1 cut(s) 1559
EcoICRI GAGCTC 1 cut(s) 361
EcoNI CCTNNNNNAGG 1 cut(s) 1814
EcoO109I RGGNCCY 4 cut(s) 258, 259, 1704, 2345
EcoRII CCWGG 2 cut(s) 116, 378
EcoT14I CCWWGG 3 cut(s) 1467, 1783, 2327
EcoT38I GRGCYC 3 cut(s) 262, 363, 1134
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 3 cut(s) 1467, 1783, 2327
Esp3I CGTCTC 2 cut(s) 352, 1022
FaeI CATG 3 cut(s) 850, 1696, 2458
FaqI GGGAC 4 cut(s) 203, 335, 1690, 1981
FatI CATG 3 cut(s) 846, 1692, 2454
FauI CCCGC 2 cut(s) 773, 1157
FauNDI CATATG 3 cut(s) 317, 881, 2120
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 297
FokI GGATG 4 cut(s) 262, 1606, 1886, 2458
FriOI GRGCYC 3 cut(s) 262, 363, 1134
FspBI CTAG 8 cut(s) 734, 768, 1005, 1188, 1634, 1971, 2382, 2448
GlaI GCGC 4 cut(s) 24, 283, 1428, 1460
GsuI CTGGAG 3 cut(s) 1161, 1355, 1439
HaeII RGCGCY 2 cut(s) 285, 1462
HaeIII GGCC 9 cut(s) 65, 237, 260, 1665, 1908, 2126, 2263, 2347, 2362
HgaI GACGC 2 cut(s) 205, 334
HhaI GCGC 4 cut(s) 25, 284, 1429, 1461
Hin1I GRCGYC 3 cut(s) 216, 282, 345
Hin1II CATG 3 cut(s) 850, 1696, 2458
Hin6I GCGC 4 cut(s) 23, 282, 1427, 1459
HinP1I GCGC 4 cut(s) 23, 282, 1427, 1459
HincII GTYRAC 2 cut(s) 247, 298
HindII GTYRAC 2 cut(s) 247, 298
HindIII AAGCTT 3 cut(s) 1375, 1484, 1916
HphI GGTGA 7 cut(s) 69, 168, 181, 600, 722, 1231, 2236
Hpy166II GTNNAC 3 cut(s) 247, 298, 1214
Hpy8I GTNNAC 3 cut(s) 247, 298, 1214
Hpy99I CGWCG 5 cut(s) 299, 302, 368, 436, 454
HpyAV CCTTC 9 cut(s) 148, 192, 903, 1820, 2067, 2137, 2288, 2336, 2445
HpyCH4III ACNGT 7 cut(s) 368, 466, 839, 1079, 1370, 1540, 2484
HpyF10VI GCNNNNNNNGC 7 cut(s) 288, 680, 1391, 1400, 1424, 1662, 1830
Hsp92I GRCGYC 3 cut(s) 216, 282, 345
Hsp92II CATG 3 cut(s) 850, 1696, 2458
HspAI GCGC 4 cut(s) 23, 282, 1427, 1459
KasI GGCGCC 1 cut(s) 281
KflI GGGWCCC 1 cut(s) 1704
Kpn2I TCCGGA 1 cut(s) 387
KpnI GGTACC 1 cut(s) 1945
Ksp22I TGATCA 1 cut(s) 814
KspI CCGCGG 1 cut(s) 343
Kzo9I GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
LguI GCTCTTC 3 cut(s) 91, 1410, 1728
LmnI GCTCC 6 cut(s) 30, 140, 308, 390, 1490, 1505
Lsp1109I GCAGC 7 cut(s) 252, 391, 658, 815, 875, 897, 2260
LweI GCATC 2 cut(s) 1090, 2480
MabI ACCWGGT 2 cut(s) 116, 378
MaeI CTAG 8 cut(s) 734, 768, 1005, 1188, 1634, 1971, 2382, 2448
MaeIII GTNAC 5 cut(s) 580, 808, 979, 1136, 2224
MalI GATC 9 cut(s) 8, 443, 484, 654, 816, 1092, 1277, 1802, 2202
MboI GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
MboII GAAGA 8 cut(s) 28, 78, 114, 961, 1230, 1285, 1397, 1745
MhlI GDGCHC 4 cut(s) 262, 363, 387, 1134
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 3 cut(s) 242, 317, 520
MmeI TCCRAC 4 cut(s) 330, 351, 969, 1224
MroI TCCGGA 1 cut(s) 387
MroXI GAANNNNTTC 2 cut(s) 1769, 2111
MseI TTAA 7 cut(s) 501, 900, 960, 1073, 1086, 1628, 1754
MslI CAYNNNNRTG 2 cut(s) 1990, 2233
MspA1I CMGCKG 5 cut(s) 342, 356, 806, 888, 1164
MspCI CTTAAG 1 cut(s) 1753
MspR9I CCNGG 6 cut(s) 81, 118, 234, 256, 380, 1152
Mva1269I GAATGC 1 cut(s) 1521
MvaI CCWGG 2 cut(s) 118, 380
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 7 cut(s) 288, 680, 1391, 1400, 1424, 1662, 1830
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 4 cut(s) 81, 234, 256, 1152
NdeI CATATG 3 cut(s) 317, 881, 2120
NdeII GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
NlaIII CATG 3 cut(s) 850, 1696, 2458
NmeAIII GCCGAG 1 cut(s) 1850
NmuCI GTSAC 2 cut(s) 979, 2224
PaeR7I CTCGAG 1 cut(s) 1559
PagI TCATGA 1 cut(s) 1692
PceI AGGCCT 1 cut(s) 2362
PciSI GCTCTTC 3 cut(s) 91, 1410, 1728
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PctI GAATGC 1 cut(s) 1521
PdmI GAANNNNTTC 2 cut(s) 1769, 2111
PfeI GAWTC 9 cut(s) 520, 559, 869, 1034, 1222, 1473, 1765, 1770, 2160
PflMI CCANNNNNTGG 1 cut(s) 1379
PinAI ACCGGT 2 cut(s) 398, 1216
Ple19I CGATCG 1 cut(s) 444
PleI GAGTC 3 cut(s) 242, 317, 520
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 3 cut(s) 242, 317, 520
PpuMI RGGWCCY 1 cut(s) 1704
PsiI TTATAA 2 cut(s) 864, 1515
Psp124BI GAGCTC 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 1704
Psp6I CCWGG 2 cut(s) 116, 378
PspGI CCWGG 2 cut(s) 116, 378
PspOMI GGGCCC 1 cut(s) 258
PspPPI RGGWCCY 1 cut(s) 1704
PspXI VCTCGAGB 1 cut(s) 1559
PstNI CAGNNNCTG 1 cut(s) 2468
PvuI CGATCG 1 cut(s) 444
PvuII CAGCTG 2 cut(s) 806, 888
RsaI GTAC 4 cut(s) 494, 1445, 1943, 2283
RsaNI GTAC 4 cut(s) 493, 1444, 1942, 2282
RseI CAYNNNNRTG 2 cut(s) 1990, 2233
SacI GAGCTC 1 cut(s) 363
SacII CCGCGG 1 cut(s) 343
SalI GTCGAC 1 cut(s) 296
SapI GCTCTTC 3 cut(s) 91, 1410, 1728
SaqAI TTAA 7 cut(s) 501, 900, 960, 1073, 1086, 1628, 1754
Sau3AI GATC 9 cut(s) 6, 441, 482, 652, 814, 1090, 1275, 1800, 2200
SchI GAGTC 3 cut(s) 242, 317, 520
ScrFI CCNGG 6 cut(s) 81, 118, 234, 256, 380, 1152
SduI GDGCHC 4 cut(s) 262, 363, 387, 1134
SexAI ACCWGGT 2 cut(s) 116, 378
SfaNI GCATC 2 cut(s) 1090, 2480
SfcI CTRYAG 1 cut(s) 1066
SfoI GGCGCC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 1559
Sfr303I CCGCGG 1 cut(s) 343
SgrAI CRCCGGYG 1 cut(s) 398
SgrBI CCGCGG 1 cut(s) 343
SgrDI CGTCGACG 1 cut(s) 296
SinI GGWCC 4 cut(s) 414, 1214, 1704, 1933
SlaI CTCGAG 1 cut(s) 1559
SmiI ATTTAAAT 1 cut(s) 1629
SmiMI CAYNNNNRTG 2 cut(s) 1990, 2233
SmlI CTYRAG 3 cut(s) 1409, 1559, 1753
SmoI CTYRAG 3 cut(s) 1409, 1559, 1753
SpeI ACTAGT 1 cut(s) 2381
SseBI AGGCCT 1 cut(s) 2362
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 8 cut(s) 734, 768, 1005, 1188, 1634, 1971, 2382, 2448
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 2362
StyD4I CCNGG 6 cut(s) 79, 116, 232, 254, 378, 1150
StyI CCWWGG 3 cut(s) 1467, 1783, 2327
SwaI ATTTAAAT 1 cut(s) 1629
TaaI ACNGT 7 cut(s) 368, 466, 839, 1079, 1370, 1540, 2484
TaqI TCGA 6 cut(s) 297, 363, 423, 906, 1560, 1768
TatI WGTACW 2 cut(s) 492, 2281
TauI GCSGC 6 cut(s) 68, 197, 216, 240, 342, 345
TfiI GAWTC 9 cut(s) 520, 559, 869, 1034, 1222, 1473, 1765, 1770, 2160
Tru1I TTAA 7 cut(s) 501, 900, 960, 1073, 1086, 1628, 1754
Tru9I TTAA 7 cut(s) 501, 900, 960, 1073, 1086, 1628, 1754
TscAI CASTG 7 cut(s) 469, 673, 824, 1338, 1554, 1795, 1963
TseFI GTSAC 2 cut(s) 979, 2224
TseI GCWGC 7 cut(s) 240, 404, 671, 803, 885, 888, 2248
Tsp45I GTSAC 2 cut(s) 979, 2224
TspDTI ATGAA 6 cut(s) 142, 989, 1185, 1681, 1709, 2397
TspGWI ACGGA 2 cut(s) 449, 1046
TspRI CASTG 7 cut(s) 469, 673, 824, 1338, 1554, 1795, 1963
Van91I CCANNNNNTGG 1 cut(s) 1379
Vha464I CTTAAG 1 cut(s) 1753
VpaK11BI GGWCC 4 cut(s) 414, 1214, 1704, 1933
XagI CCTNNNNNAGG 1 cut(s) 1814
XapI RAATTY 6 cut(s) 11, 852, 1748, 2077, 2135, 2418
XhoI CTCGAG 1 cut(s) 1559
XmiI GTMKAC 1 cut(s) 297
XmnI GAANNNNTTC 2 cut(s) 1769, 2111
XspI CTAG 8 cut(s) 734, 768, 1005, 1188, 1634, 1971, 2382, 2448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.