Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010037

Physical Location & Seq

Genomic Coordinates Forward (+)

6678472 .. 6681021

Gene Structure 2550 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1039500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGCGCTCCAAACCCATATCAGTCTTTCACACCTTCTCTTGATTGCCTCTCTCCTCTCCGCCGCCGTTCCCGCCGCCACCACAATCTCACCAGGCTCCTCTCTCTCCGCCGCCAACCCAACCACCACCTGGTCCTCCCCAAATGACACCTTCTCCCTCGGCTTCATCCCCTCCGACCCCCCCACTTCACCCCCTTCTTACATCGCCGCCATCTCCTACTCCGGCGGCGTACCCGTCTGGTCCGCCGGTGCAGGCAAAGCAGTTGATTCCGGTGGAACCCTCCAGTTCCTCCCATCCGGCACCCTTCGCCTCGTCAACGGCTCTGGCGCCACTCTCTGGGACTCCAACACCACCAGCCGCGGCGTCTCTTCGGCTGGGATTGACGATTCGGGCAACTTGGTACTTCGAAACGGCACCGTTACGGTCTGGTCCAGCTTCGACAACCCCACCGATTCGATCGTCCCTGGGCAGAACTTCACTGTGAGTAAGGTTTTGCGGTCTGGGTTGTACTCGTTTAAGCTTGTAAAAAACGGGAACCTTACACTTTTGTGGAATGATATTACTGTGTATTGGAATCAGGGATTGAATTCTTCTGTTAATACGAATTTGAGTTCGCCGACTCTAGGATTGCGGTCAATTGGTTTTCTGTCAATTTCTGATCCACAATTGTCCACTGATGCTATTGTTGCTTATAGTAGTGATTATGCCGAAGTGGGTGATATTTTGCGGTTTTTGAAGTTGGGGAATGATGGGAATCTGAGGATTTATAGCTCTACTAGAGGTAGTGGGACCGTAACTGAGAGATGGGCAGCTGTTACTGATCAGTGTGAGGTTTTCGGGTATTGTGGGGACATGGGAGTTTGTACTTATAACGATACTGATCCGGTATGCGGATGTATGTCTCTTAATTTTGAGCTTGTTGATCCCAAGGATAGCAGGAAAGGGTGCAAGAGGAAGATGGAGATTAAGGATTGTCCTCAGAGTGTGATTATGTTGGACTTGGAACATACTCGGTTCTTGTCGTACCCTCCTGAGACAGACTCGCAGATTTTCTTTTTCTCTGCGGGAATATCGGCTTGCAGGTTGAATTGTCTTGGAAGTAGTGCTTGTGATGCTTCGACGTCTTTGTCTGATGGCACCGGGCTATGTTATTCCAAGACACCAGGTTTTCTGAGTGGGTATCACGGTCCAGCTATGTCCAGTAGTTCATATATCAAGGTATGTGGACCAGTGGTTCCAAATCCATCATCATCACTGGGGACTGCTGGCAACAAAAAAGATGGGAAGTTGCGTGCTTGGATAGTGGTTGTTGTTGTTATTGCCACCCTCTTTGGTTTGATGGCATTTGAAGGTGGTTTGTGGTGGTGGTTTTGCAGAAACAGTCGCAGCTTTGGTGGATTGTCTGCTCAATATGCGCTTCTTGAGTATGCTTCTGGTGCACCGGTTCAGTTCTTGTATAAGGAGCTCCAACGCTCAACGAAGGGATTCAAGGAGAAGCTTGGGGCAGGGGGATTTGGGACTGTTTATAAAGGCATTCTTGCTAATAGAACTGTTGTTGCAGTTAAGCAACTTGAGGGGATTGAGCAAGGGGAGAAACAGTTCAGGATGGAGGTTGCAACAATTAGTAGCACACACCATTTGAATCTGGTGAGGCTGATTGGTTTTTGCTCTGAGGGGCGCCATAGGCTTCTGGTATATGAGTTCATGAAAAATGGGTCCCTTGATAGTTTCCTTTTTGCAACAGAAGAGCAATCAGGAAAGTTGTTGAATTGGGAGTCTCGGTTCAACGTTGCCCTCGGCACTGCAAGGGGGATCACATACCTTCATGAGGAGTGCCGAGACTGCATTGTGCATTGTGATATAAAACCGGAGAACATTCTGTTAGATGAGAATTACAATGCCAAAGTGTCAGATTTTGGCCTCGCAAAGCTCGTTAATCCAAAGGACCATAGGTATCGAACCTTGACAAGTGTTAGAGGTACTAGAGGGTATTTGGCACCAGAATGGCTGGCAAATCTTCCAATAACTTCCAAATCTGATGTTTACAGTTACGGTATGGTTTTGCTAGAGATAGTGAGTGGGAGAAGGAATTTTGAAGTTTCTGAGGCGACAAATAGGAAGAAGTTGTCCTTATGGGCTTTTGAGGAATTTGAGAAGGGTAACATCAAGGGAATTGTCGATAGAATTGTCGATAGAAGGTTAGTCGACCCAGATGTTGATATGGATCAAGTGAAGAGAGCAATTCAGGTAACCTTCTGGTGCATACAGGAACAACCATCTCACAGGCCAATGATGGGAAAGGTTGTGCAGATGCTGGAAGGAATTACAGATATTGAAAACCCCCCAGACCCAAGGGCTGTCATCGAAGGGTCTGCCAGTGGAACCAGCATGACTGTGAGCAGTAATATCAGCCCTTTGTCCACTGCCGCAGGCTCAGCCCCAGCTCCCTCCTCATTTTCATCATTCCAAATTTCGGGAGTATCGCCTCTAACATCAGGGAGGAATACGGAGAAGGCAACTGCATCCCTTATACAGTCCGACCCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

850

Amino Acids

91.73

Weight (kDa)

6.45

Isoelectric Point (pI)

40.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	79 - 164	1.1e-12	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	496 - 772	5.3e-46	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	497 - 770	1.6e-50	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010037)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34300
fragaria_vesca	FvH4_2g12390
malus_domestica	MD05G1039400.v1.1 MD05G1039500.v1.1 MD10G1044900.v1.1
prunus_persica	Prupe.8G051900_v2.0.a1
pyrus_communis	pycom05g03200 pycom05g03220 pycom10g03130 pycom10g03140
rosa_chinensis	RchiOBHm_Chr6g0272421
rosa_laevigata	RLG00000013671
rosa_multiflora	Rmu_co8482727.1_g000001 Rmu_sc0017109.1_g000001 Rmu_ssc0000204.1_g000064
rosa_rugosa	Rorug06G0069700
rosa_samantha	Rh6AG185500 Rh6BG188600 Rh6CG186600 Rh6DG178200
rosa_wichuraiana	Rw6G016020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	870, 1527
AasI	GACNNNNNNGTC	1 cut(s)	1126
AatII	GACGTC	1 cut(s)	1124
Acc36I	ACCTGC	1 cut(s)	1071
AccB1I	GGYRCC	6 cut(s)	299, 326, 413, 1136, 1677, 1996
AccB7I	CCANNNNNTGG	2 cut(s)	129, 336
AccI	GTMKAC	1 cut(s)	2205
AccII	CGCG	1 cut(s)	360
AclI	AACGTT	1 cut(s)	1788
AclWI	GGATC	5 cut(s)	653, 875, 917, 1820, 2232
AcsI	RAATTY	5 cut(s)	586, 604, 2089, 2147, 2469
AcyI	GRCGYC	4 cut(s)	327, 363, 1121, 1678
AfaI	GTAC	6 cut(s)	231, 402, 509, 865, 1025, 1981
AfiI	CCNNNNNNNGG	7 cut(s)	129, 336, 623, 890, 1828, 2294, 2473
AgeI	ACCGGT	1 cut(s)	1441
AjnI	CCWGG	4 cut(s)	91, 128, 463, 1162
AleI	CACNNNNGTG	1 cut(s)	547
AloI	GAACNNNNNNTCC	6 cut(s)	1218, 1250, 1583, 1615, 1766, 1798
Alw21I	GWGCWC	2 cut(s)	1441, 1467
Alw26I	GTCTC	5 cut(s)	370, 906, 1028, 1782, 1833
Alw44I	GTGCAC	1 cut(s)	1437
AlwI	GGATC	5 cut(s)	653, 875, 917, 1820, 2232
AlwNI	CAGNNNCTG	1 cut(s)	2314
AoxI	GGCC	2 cut(s)	1918, 2285
ApaLI	GTGCAC	1 cut(s)	1437
ApeKI	GCWGC	2 cut(s)	809, 1386
ApoI	RAATTY	5 cut(s)	586, 604, 2089, 2147, 2469
AsiGI	ACCGGT	1 cut(s)	1441
Asp700I	GAANNNNTTC	2 cut(s)	1484, 1598
AspLEI	GCGC	4 cut(s)	7, 329, 1417, 1680
AspS9I	GGNCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
AsuC2I	CCSGG	1 cut(s)	1141
AsuHPI	GGTGA	4 cut(s)	81, 180, 728, 1660
AsuII	TTCGAA	1 cut(s)	406
AvaII	GGWCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
BaeGI	GKGCMC	1 cut(s)	1441
BanI	GGYRCC	6 cut(s)	299, 326, 413, 1136, 1677, 1996
BanII	GRGCYC	1 cut(s)	1467
Bbv12I	GWGCWC	2 cut(s)	1441, 1467
BbvI	GCAGC	2 cut(s)	821, 1398
BceAI	ACGGC	3 cut(s)	50, 334, 427
BcgI	CGANNNNNNTGC	2 cut(s)	2354, 2388
BciT130I	CCWGG	4 cut(s)	93, 130, 465, 1164
BclI	TGATCA	1 cut(s)	820
BcnI	CCSGG	1 cut(s)	1141
BcoDI	GTCTC	5 cut(s)	370, 906, 1028, 1782, 1833
BfaI	CTAG	4 cut(s)	623, 777, 1983, 2066
BfoI	RGCGCY	3 cut(s)	8, 330, 1681
BfuAI	ACCTGC	1 cut(s)	1071
BlpI	GCTNAGC	1 cut(s)	2434
Bme1390I	CCNGG	5 cut(s)	93, 130, 465, 1141, 1164
Bme18I	GGWCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
BmgT120I	GGNCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
BmrFI	CCNGG	5 cut(s)	93, 130, 465, 1141, 1164
BmrI	ACTGGG	1 cut(s)	1265
BmsI	GCATC	4 cut(s)	667, 1102, 2301, 2531
BmuI	ACTGGG	1 cut(s)	1265
BplI	GAGNNNNNCTC	2 cut(s)	1025, 1057
BpmI	CTGGAG	1 cut(s)	266
Bpu1102I	GCTNAGC	1 cut(s)	2434
Bpu14I	TTCGAA	1 cut(s)	406
BpuEI	CTTGAG	2 cut(s)	1442, 1592
BpuMI	CCSGG	1 cut(s)	1141
BsaBI	GATNNNNATC	3 cut(s)	753, 879, 2223
BsaHI	GRCGYC	4 cut(s)	327, 363, 1121, 1678
BsaJI	CCNNGG	7 cut(s)	157, 358, 463, 464, 927, 1795, 2351
BsaWI	WCCGGW	4 cut(s)	269, 883, 1441, 1867
BsaXI	ACNNNNNCTCC	6 cut(s)	137, 167, 1583, 1613, 1766, 1796
Bsc4I	CCNNNNNNNGG	7 cut(s)	129, 336, 623, 890, 1828, 2294, 2473
Bse118I	RCCGGY	2 cut(s)	245, 1441
Bse1I	ACTGG	5 cut(s)	283, 1200, 1229, 1260, 2376
Bse8I	GATNNNNATC	3 cut(s)	753, 879, 2223
BseBI	CCWGG	4 cut(s)	93, 130, 465, 1164
BseDI	CCNNGG	7 cut(s)	157, 358, 463, 464, 927, 1795, 2351
BseGI	GGATG	5 cut(s)	165, 293, 899, 1611, 2522
BseJI	GATNNNNATC	3 cut(s)	753, 879, 2223
BseLI	CCNNNNNNNGG	7 cut(s)	129, 336, 623, 890, 1828, 2294, 2473
BseMII	CTCAG	8 cut(s)	749, 789, 992, 1023, 1163, 1662, 2094, 2448
BseNI	ACTGG	5 cut(s)	283, 1200, 1229, 1260, 2376
BseRI	GAGGAG	4 cut(s)	44, 88, 1844, 2440
BseSI	GKGCMC	1 cut(s)	1441
BseXI	GCAGC	2 cut(s)	821, 1398
BseYI	CCCAGC	2 cut(s)	374, 2440
BsgI	GTGCAG	2 cut(s)	270, 2327
Bsh1236I	CGCG	1 cut(s)	360
Bsh1285I	CGRYCG	1 cut(s)	459
BshFI	GGCC	2 cut(s)	1920, 2287
BshNI	GGYRCC	6 cut(s)	299, 326, 413, 1136, 1677, 1996
BshTI	ACCGGT	1 cut(s)	1441
BsiEI	CGRYCG	1 cut(s)	459
BsiHKAI	GWGCWC	2 cut(s)	1441, 1467
BsiSI	CCGG	8 cut(s)	222, 246, 270, 297, 884, 1140, 1442, 1868
BslFI	GGGAC	7 cut(s)	353, 446, 802, 863, 1273, 1531, 1702
BslI	CCNNNNNNNGG	7 cut(s)	129, 336, 623, 890, 1828, 2294, 2473
BsmAI	GTCTC	5 cut(s)	370, 906, 1028, 1782, 1833
BsmBI	CGTCTC	1 cut(s)	370
BsmFI	GGGAC	7 cut(s)	353, 446, 802, 863, 1273, 1531, 1702
BsmI	GAATGC	1 cut(s)	1533
BsnI	GGCC	2 cut(s)	1920, 2287
Bsp119I	TTCGAA	1 cut(s)	406
Bsp1286I	GDGCHC	2 cut(s)	1441, 1467
Bsp143I	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
Bsp1720I	GCTNAGC	1 cut(s)	2434
BspANI	GGCC	2 cut(s)	1920, 2287
BspCNI	CTCAG	8 cut(s)	750, 790, 991, 1024, 1164, 1663, 2095, 2447
BspFNI	CGCG	1 cut(s)	360
BspHI	TCATGA	2 cut(s)	1704, 1825
BspMI	ACCTGC	1 cut(s)	1071
BspPI	GGATC	5 cut(s)	653, 875, 917, 1820, 2232
BspQI	GCTCTTC	1 cut(s)	1740
BspT104I	TTCGAA	1 cut(s)	406
BspT107I	GGYRCC	6 cut(s)	299, 326, 413, 1136, 1677, 1996
BsrFI	RCCGGY	2 cut(s)	245, 1441
BsrI	ACTGG	5 cut(s)	283, 1200, 1229, 1260, 2376
BssAI	RCCGGY	2 cut(s)	245, 1441
BssECI	CCNNGG	7 cut(s)	157, 358, 463, 464, 927, 1795, 2351
BssMI	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
BssNI	GRCGYC	4 cut(s)	327, 363, 1121, 1678
BssT1I	CCWWGG	2 cut(s)	927, 2351
Bst2UI	CCWGG	4 cut(s)	93, 130, 465, 1164
Bst6I	CTCTTC	3 cut(s)	373, 1740, 2228
BstACI	GRCGYC	4 cut(s)	327, 363, 1121, 1678
BstBI	TTCGAA	1 cut(s)	406
BstC8I	GCNNGC	6 cut(s)	253, 1078, 1267, 1293, 2010, 2431
BstDEI	CTNAG	8 cut(s)	758, 798, 978, 1032, 1172, 1671, 2103, 2434
BstDSI	CCRYGG	1 cut(s)	358
BstEII	GGTNACC	1 cut(s)	2248
BstENI	CCTNNNNNAGG	1 cut(s)	1826
BstF5I	GGATG	5 cut(s)	165, 293, 899, 1611, 2522
BstFNI	CGCG	1 cut(s)	360
BstH2I	RGCGCY	3 cut(s)	8, 330, 1681
BstHHI	GCGC	4 cut(s)	7, 329, 1417, 1680
BstKTI	GATC	7 cut(s)	459, 661, 823, 883, 925, 1815, 2227
BstMAI	GTCTC	5 cut(s)	370, 906, 1028, 1782, 1833
BstMBI	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
BstMCI	CGRYCG	1 cut(s)	459
BstNI	CCWGG	4 cut(s)	93, 130, 465, 1164
BstPI	GGTNACC	1 cut(s)	2248
BstSCI	CCNGG	5 cut(s)	91, 128, 463, 1139, 1162
BstSLI	GKGCMC	1 cut(s)	1441
BstUI	CGCG	1 cut(s)	360
BstV1I	GCAGC	2 cut(s)	821, 1398
BsuRI	GGCC	2 cut(s)	1920, 2287
BtgI	CCRYGG	1 cut(s)	358
BtgZI	GCGATG	1 cut(s)	187
BtsCI	GGATG	5 cut(s)	165, 293, 899, 1611, 2522
BtsI	GCAGTG	2 cut(s)	1800, 2421
BtsIMutI	CAGTG	8 cut(s)	477, 672, 830, 1236, 1253, 1800, 2383, 2421
BveI	ACCTGC	1 cut(s)	1071
Cac8I	GCNNGC	6 cut(s)	253, 1078, 1267, 1293, 2010, 2431
CaiI	CAGNNNCTG	1 cut(s)	2314
CciI	TCATGA	2 cut(s)	1704, 1825
CfoI	GCGC	4 cut(s)	7, 329, 1417, 1680
Cfr10I	RCCGGY	2 cut(s)	245, 1441
Cfr13I	GGNCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
Cfr42I	CCGCGG	1 cut(s)	361
CseI	GACGC	1 cut(s)	352
CsiI	ACCWGGT	2 cut(s)	128, 1162
Csp6I	GTAC	6 cut(s)	230, 401, 508, 864, 1024, 1980
CspAI	ACCGGT	1 cut(s)	1441
CspCI	CAANNNNNGTGG	4 cut(s)	1204, 1239, 1312, 1347
CviAII	CATG	4 cut(s)	853, 1705, 1826, 2389
CviQI	GTAC	6 cut(s)	230, 401, 508, 864, 1024, 1980
DdeI	CTNAG	8 cut(s)	758, 798, 978, 1032, 1172, 1671, 2103, 2434
DinI	GGCGCC	2 cut(s)	328, 1679
DpnI	GATC	7 cut(s)	458, 660, 822, 882, 924, 1814, 2226
DpnII	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
DrdI	GACNNNNNNGTC	1 cut(s)	1126
DseDI	GACNNNNNNGTC	1 cut(s)	1126
Eam1104I	CTCTTC	3 cut(s)	373, 1740, 2228
EarI	CTCTTC	3 cut(s)	373, 1740, 2228
EciI	GGCGGA	3 cut(s)	49, 97, 232
Ecl136II	GAGCTC	1 cut(s)	1465
Eco130I	CCWWGG	2 cut(s)	927, 2351
Eco24I	GRGCYC	1 cut(s)	1467
Eco47I	GGWCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
Eco53kI	GAGCTC	1 cut(s)	1465
Eco91I	GGTNACC	1 cut(s)	2248
EcoICRI	GAGCTC	1 cut(s)	1465
EcoNI	CCTNNNNNAGG	1 cut(s)	1826
EcoO109I	RGGNCCY	1 cut(s)	1716
EcoO65I	GGTNACC	1 cut(s)	2248
EcoRI	GAATTC	1 cut(s)	586
EcoRII	CCWGG	4 cut(s)	91, 128, 463, 1162
EcoT14I	CCWWGG	2 cut(s)	927, 2351
EcoT38I	GRGCYC	1 cut(s)	1467
EgeI	GGCGCC	2 cut(s)	328, 1679
EheI	GGCGCC	2 cut(s)	328, 1679
ErhI	CCWWGG	2 cut(s)	927, 2351
Esp3I	CGTCTC	1 cut(s)	370
FaeI	CATG	4 cut(s)	856, 1708, 1829, 2392
FalI	AAGNNNNNCTT	6 cut(s)	1090, 1122, 1521, 1553, 2114, 2146
FaqI	GGGAC	7 cut(s)	353, 446, 802, 863, 1273, 1531, 1702
FatI	CATG	4 cut(s)	852, 1704, 1825, 2388
FauI	CCCGC	2 cut(s)	79, 1057
FbaI	TGATCA	1 cut(s)	820
FblI	GTMKAC	1 cut(s)	2205
FokI	GGATG	5 cut(s)	152, 280, 906, 1618, 2509
FriOI	GRGCYC	1 cut(s)	1467
FspBI	CTAG	4 cut(s)	623, 777, 1983, 2066
GlaI	GCGC	4 cut(s)	6, 328, 1416, 1679
GsaI	CCCAGC	2 cut(s)	378, 2444
GsuI	CTGGAG	1 cut(s)	266
HaeII	RGCGCY	3 cut(s)	8, 330, 1681
HaeIII	GGCC	2 cut(s)	1920, 2287
HapII	CCGG	8 cut(s)	222, 246, 270, 297, 884, 1140, 1442, 1868
HgaI	GACGC	1 cut(s)	352
HhaI	GCGC	4 cut(s)	7, 329, 1417, 1680
Hin1I	GRCGYC	4 cut(s)	327, 363, 1121, 1678
Hin1II	CATG	4 cut(s)	856, 1708, 1829, 2392
Hin6I	GCGC	4 cut(s)	5, 327, 1415, 1678
HinP1I	GCGC	4 cut(s)	5, 327, 1415, 1678
HincII	GTYRAC	2 cut(s)	316, 2206
HindII	GTYRAC	2 cut(s)	316, 2206
HindIII	AAGCTT	2 cut(s)	518, 1496
HpaII	CCGG	8 cut(s)	222, 246, 270, 297, 884, 1140, 1442, 1868
HphI	GGTGA	4 cut(s)	81, 180, 728, 1660
Hpy166II	GTNNAC	7 cut(s)	316, 672, 1226, 1439, 2044, 2206, 2421
Hpy188III	TCNNGA	8 cut(s)	40, 1031, 1421, 1603, 1705, 1755, 1826, 2475
Hpy8I	GTNNAC	7 cut(s)	316, 672, 1226, 1439, 2044, 2206, 2421
Hpy99I	CGWCG	1 cut(s)	1123
HpyCH4IV	ACGT	2 cut(s)	1121, 1788
HpyF3I	CTNAG	8 cut(s)	758, 798, 978, 1032, 1172, 1671, 2103, 2434
HpySE526I	ACGT	2 cut(s)	1121, 1788
Hsp92I	GRCGYC	4 cut(s)	327, 363, 1121, 1678
Hsp92II	CATG	4 cut(s)	856, 1708, 1829, 2392
HspAI	GCGC	4 cut(s)	5, 327, 1415, 1678
KasI	GGCGCC	2 cut(s)	326, 1677
KflI	GGGWCCC	1 cut(s)	1716
Ksp22I	TGATCA	1 cut(s)	820
KspI	CCGCGG	1 cut(s)	361
Kzo9I	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
LguI	GCTCTTC	1 cut(s)	1740
LmnI	GCTCC	5 cut(s)	12, 101, 1462, 1470, 2449
Lsp1109I	GCAGC	2 cut(s)	821, 1398
LweI	GCATC	4 cut(s)	667, 1102, 2301, 2531
MabI	ACCWGGT	2 cut(s)	128, 1162
MaeI	CTAG	4 cut(s)	623, 777, 1983, 2066
MaeII	ACGT	2 cut(s)	1121, 1788
MaeIII	GTNAC	6 cut(s)	418, 793, 814, 2048, 2159, 2248
MalI	GATC	7 cut(s)	458, 660, 822, 882, 924, 1814, 2226
MboI	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
MboII	GAAGA	7 cut(s)	360, 582, 967, 1757, 2009, 2131, 2245
MfeI	CAATTG	2 cut(s)	636, 665
MhlI	GDGCHC	2 cut(s)	1441, 1467
Mly113I	GGCGCC	2 cut(s)	327, 1678
MlyI	GAGTC	4 cut(s)	335, 613, 1034, 1784
MmeI	TCCRAC	4 cut(s)	198, 369, 975, 1492
MroXI	GAANNNNTTC	2 cut(s)	1484, 1598
MseI	TTAA	6 cut(s)	516, 597, 906, 966, 1563, 1935
MslI	CAYNNNNRTG	3 cut(s)	547, 2002, 2393
MspA1I	CMGCKG	2 cut(s)	360, 812
MspI	CCGG	8 cut(s)	222, 246, 270, 297, 884, 1140, 1442, 1868
MspR9I	CCNGG	5 cut(s)	93, 130, 465, 1141, 1164
MunI	CAATTG	2 cut(s)	636, 665
Mva1269I	GAATGC	1 cut(s)	1533
MvaI	CCWGG	4 cut(s)	93, 130, 465, 1164
MvnI	CGCG	1 cut(s)	360
NarI	GGCGCC	2 cut(s)	327, 1678
NciI	CCSGG	1 cut(s)	1141
NdeII	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
NlaIII	CATG	4 cut(s)	856, 1708, 1829, 2392
NmeAIII	GCCGAG	3 cut(s)	138, 1776, 1862
NspV	TTCGAA	1 cut(s)	406
OliI	CACNNNNGTG	1 cut(s)	547
PagI	TCATGA	2 cut(s)	1704, 1825
PasI	CCCWGGG	1 cut(s)	464
PciSI	GCTCTTC	1 cut(s)	1740
PcsI	WCGNNNNNNNCGW	1 cut(s)	1929
PctI	GAATGC	1 cut(s)	1533
PdmI	GAANNNNTTC	2 cut(s)	1484, 1598
PfeI	GAWTC	7 cut(s)	266, 386, 452, 574, 754, 1485, 1642
PflMI	CCANNNNNTGG	2 cut(s)	129, 336
PinAI	ACCGGT	1 cut(s)	1441
Ple19I	CGATCG	1 cut(s)	459
PleI	GAGTC	4 cut(s)	335, 613, 1034, 1783
PluTI	GGCGCC	2 cut(s)	330, 1681
PpsI	GAGTC	4 cut(s)	335, 613, 1034, 1783
PpuMI	RGGWCCY	1 cut(s)	1716
PsiI	TTATAA	2 cut(s)	870, 1527
Psp124BI	GAGCTC	1 cut(s)	1467
Psp1406I	AACGTT	1 cut(s)	1788
Psp5II	RGGWCCY	1 cut(s)	1716
Psp6I	CCWGG	4 cut(s)	91, 128, 463, 1162
PspEI	GGTNACC	1 cut(s)	2248
PspFI	CCCAGC	2 cut(s)	374, 2440
PspGI	CCWGG	4 cut(s)	91, 128, 463, 1162
PspPI	GGNCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
PspPPI	RGGWCCY	1 cut(s)	1716
PstNI	CAGNNNCTG	1 cut(s)	2314
PvuI	CGATCG	1 cut(s)	459
PvuII	CAGCTG	1 cut(s)	812
RsaI	GTAC	6 cut(s)	231, 402, 509, 865, 1025, 1981
RsaNI	GTAC	6 cut(s)	230, 401, 508, 864, 1024, 1980
RseI	CAYNNNNRTG	3 cut(s)	547, 2002, 2393
SacI	GAGCTC	1 cut(s)	1467
SacII	CCGCGG	1 cut(s)	361
SalI	GTCGAC	1 cut(s)	2204
SapI	GCTCTTC	1 cut(s)	1740
SaqAI	TTAA	6 cut(s)	516, 597, 906, 966, 1563, 1935
Sau3AI	GATC	7 cut(s)	456, 658, 820, 880, 922, 1812, 2224
Sau96I	GGNCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
SchI	GAGTC	4 cut(s)	335, 613, 1034, 1784
ScrFI	CCNGG	5 cut(s)	93, 130, 465, 1141, 1164
SduI	GDGCHC	2 cut(s)	1441, 1467
SexAI	ACCWGGT	2 cut(s)	128, 1162
SfaNI	GCATC	4 cut(s)	667, 1102, 2301, 2531
SfoI	GGCGCC	2 cut(s)	328, 1679
Sfr303I	CCGCGG	1 cut(s)	361
SfuI	TTCGAA	1 cut(s)	406
SgrAI	CRCCGGYG	1 cut(s)	245
SgrBI	CCGCGG	1 cut(s)	361
SinI	GGWCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
SmiMI	CAYNNNNRTG	3 cut(s)	547, 2002, 2393
SmlI	CTYRAG	2 cut(s)	1421, 1571
SmoI	CTYRAG	2 cut(s)	1421, 1571
SspDI	GGCGCC	2 cut(s)	326, 1677
SspMI	CTAG	4 cut(s)	623, 777, 1983, 2066
SstI	GAGCTC	1 cut(s)	1467
StyD4I	CCNGG	5 cut(s)	91, 128, 463, 1139, 1162
StyI	CCWWGG	2 cut(s)	927, 2351
TaiI	ACGT	2 cut(s)	1124, 1791
TaqI	TCGA	9 cut(s)	406, 438, 455, 1118, 1957, 2178, 2190, 2205, 2364
TatI	WGTACW	2 cut(s)	507, 863
TauI	GCSGC	8 cut(s)	65, 77, 113, 209, 228, 360, 363, 2429
TfiI	GAWTC	7 cut(s)	266, 386, 452, 574, 754, 1485, 1642
Tru1I	TTAA	6 cut(s)	516, 597, 906, 966, 1563, 1935
Tru9I	TTAA	6 cut(s)	516, 597, 906, 966, 1563, 1935
TscAI	CASTG	8 cut(s)	484, 679, 830, 1236, 1260, 1807, 2383, 2428
TseI	GCWGC	2 cut(s)	809, 1386
TspDTI	ATGAA	6 cut(s)	154, 1197, 1693, 1721, 1814, 2448
TspGWI	ACGGA	1 cut(s)	2522
TspRI	CASTG	8 cut(s)	484, 679, 830, 1236, 1260, 1807, 2383, 2428
Van91I	CCANNNNNTGG	2 cut(s)	129, 336
VneI	GTGCAC	1 cut(s)	1437
VpaK11BI	GGWCC	8 cut(s)	132, 240, 429, 789, 1187, 1226, 1716, 1945
XagI	CCTNNNNNAGG	1 cut(s)	1826
XapI	RAATTY	5 cut(s)	586, 604, 2089, 2147, 2469
XcmI	CCANNNNNNNNNTGG	1 cut(s)	126
XmiI	GTMKAC	1 cut(s)	2205
XmnI	GAANNNNTTC	2 cut(s)	1484, 1598
XspI	CTAG	4 cut(s)	623, 777, 1983, 2066
ZraI	GACGTC	1 cut(s)	1122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.