MD05G1041300.v1.1
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008740
Physical Location & Seq
Forward (+)
6967698 .. 6971168
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1041300.v1.1.491

Sequence Viewer

Length: 606 bp
ATGATTGTGCAACACGCTTTTGCTTTTCTACTGTTGAATTTGCCTGATGTTGTAAATACGGCACCGAATGTCAGATTGCGTAATTTTTCACAAAATCCAAACAGAGTAGCTTGCTCACCAGGTTTGTCTACCAAGAAATGGGTATTACACTCAACTGCAGAGATTGAAAATACGACTTTGAAAGATGAAGACAGGAAGGCATGGGAAACATGCCGGGAAGCTCTATCTGCATTTAGTATCAGCAATGAAGAGGGAGAGAAGATACTTGGAAAAGCATTTGGCCTGGTTCATTCACCGTACTGGGGTGAAAAACGGAAAACTGAAGTACCAAAACTTGAGATTGTAATCGAAACACTGGACTATCTTCGGAGTTTAAGCCTTTCTGACGATGACCTGTGTAAGTTGCTAAAAAAGTTTCCAGAGGTTCTTGGATGTGATCTTGAACAGGAGGTGAAAATCAATGTGCAGATCTTGGAAAAGGAGTGGGGAATAAAAGGGAAATCTCTCCGAAACCTCCTTCTTCGAAATCCAAGAGTATTGGGTTTCAATGTTGATTGCAAGGGAGATTGTATGGCACAGTGCACGCGATGCTGGGTTCGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

202

Amino Acids

22.93

Weight (kDa)

8.09

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 119 - 184 9.7e-09 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccB7I CCANNNNNTGG 1 cut(s) 138
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 586
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 2 cut(s) 299, 327
AfiI CCNNNNNNNGG 2 cut(s) 138, 302
AgsI TTSAA 5 cut(s) 37, 167, 181, 443, 547
AjnI CCWGG 2 cut(s) 118, 282
AluBI AGCT 2 cut(s) 110, 221
AluI AGCT 2 cut(s) 110, 221
Alw21I GWGCWC 1 cut(s) 584
Alw44I GTGCAC 1 cut(s) 580
AoxI GGCC 1 cut(s) 280
ApaLI GTGCAC 1 cut(s) 580
ApoI RAATTY 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 108, 285, 317, 463
AsuII TTCGAA 1 cut(s) 523
BaeGI GKGCMC 1 cut(s) 584
BaeI ACNNNNGTAYC 2 cut(s) 309, 342
BanI GGYRCC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 120, 284
BcnI CCSGG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 156
BglII AGATCT 1 cut(s) 468
Bme1390I CCNGG 3 cut(s) 120, 215, 284
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 3 cut(s) 120, 215, 284
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 578
BmuI ACTGGG 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 344
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 302
Bse1I ACTGG 2 cut(s) 305, 360
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 2 cut(s) 120, 284
BseGI GGATG 1 cut(s) 437
BseJI GATNNNNATC 1 cut(s) 344
BseLI CCNNNNNNNGG 2 cut(s) 138, 302
BseMI GCAATG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 305, 360
BseSI GKGCMC 1 cut(s) 584
BseYI CCCAGC 1 cut(s) 591
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 586
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 584
BsiSI CCGG 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 138, 302
BsnI GGCC 1 cut(s) 282
Bsp119I TTCGAA 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 2 cut(s) 436, 468
BspANI GGCC 1 cut(s) 282
BspFNI CGCG 1 cut(s) 586
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 2 cut(s) 305, 360
BssMI GATC 2 cut(s) 436, 468
Bst2UI CCWGG 2 cut(s) 120, 284
Bst4CI ACNGT 3 cut(s) 33, 297, 579
Bst6I CTCTTC 1 cut(s) 243
BstAPI GCANNNNNTGC 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 523
BstC8I GCNNGC 2 cut(s) 112, 584
BstF5I GGATG 1 cut(s) 437
BstFNI CGCG 1 cut(s) 586
BstKTI GATC 2 cut(s) 439, 471
BstMBI GATC 2 cut(s) 436, 468
BstMWI GCNNNNNNNGC 2 cut(s) 227, 588
BstNI CCWGG 2 cut(s) 120, 284
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 3 cut(s) 118, 213, 282
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 584
BstUI CGCG 1 cut(s) 586
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 601
BtsCI GGATG 1 cut(s) 437
BtsIMutI CAGTG 2 cut(s) 353, 584
Cac8I GCNNGC 2 cut(s) 112, 584
CsiI ACCWGGT 1 cut(s) 118
Csp6I GTAC 2 cut(s) 298, 326
CviAII CATG 2 cut(s) 201, 210
CviJI RGCY 4 cut(s) 110, 221, 282, 378
CviKI_1 RGCY 4 cut(s) 110, 221, 282, 378
CviQI GTAC 2 cut(s) 298, 326
DpnI GATC 2 cut(s) 438, 470
DpnII GATC 2 cut(s) 436, 468
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 118, 282
FaeI CATG 2 cut(s) 204, 213
FaiI YATR 3 cut(s) 202, 211, 572
FatI CATG 2 cut(s) 200, 209
FblI GTMKAC 1 cut(s) 128
FokI GGATG 1 cut(s) 444
GsaI CCCAGC 1 cut(s) 595
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 214
Hin1II CATG 2 cut(s) 204, 213
HpaII CCGG 1 cut(s) 214
HphI GGTGA 4 cut(s) 108, 285, 317, 463
Hpy166II GTNNAC 2 cut(s) 129, 582
Hpy188I TCNGA 4 cut(s) 74, 369, 385, 509
Hpy188III TCNNGA 2 cut(s) 419, 440
Hpy8I GTNNAC 2 cut(s) 129, 582
HpyAV CCTTC 2 cut(s) 190, 527
HpyCH4III ACNGT 3 cut(s) 33, 297, 579
HpyCH4V TGCA 6 cut(s) 10, 158, 230, 466, 558, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 588
Hsp92II CATG 2 cut(s) 204, 213
Kzo9I GATC 2 cut(s) 436, 468
LweI GCATC 1 cut(s) 578
MabI ACCWGGT 1 cut(s) 118
MalI GATC 2 cut(s) 438, 470
MboI GATC 2 cut(s) 436, 468
MboII GAAGA 5 cut(s) 200, 260, 271, 356, 512
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 584
MluCI AATT 2 cut(s) 37, 82
MnlI CCTC 4 cut(s) 244, 415, 442, 524
MseI TTAA 1 cut(s) 374
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 3 cut(s) 120, 215, 284
MvaI CCWGG 2 cut(s) 120, 284
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 227, 588
NciI CCSGG 1 cut(s) 215
NdeII GATC 2 cut(s) 436, 468
NlaIII CATG 2 cut(s) 204, 213
NlaIV GGNNCC 1 cut(s) 63
NspI RCATGY 1 cut(s) 213
NspV TTCGAA 1 cut(s) 523
PflMI CCANNNNNTGG 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 118, 282
PspFI CCCAGC 1 cut(s) 591
PspGI CCWGG 2 cut(s) 118, 282
PspN4I GGNNCC 1 cut(s) 63
PstI CTGCAG 1 cut(s) 160
PsuI RGATCY 1 cut(s) 468
RsaI GTAC 2 cut(s) 299, 327
RsaNI GTAC 2 cut(s) 298, 326
SaqAI TTAA 1 cut(s) 374
Sau3AI GATC 2 cut(s) 436, 468
ScrFI CCNGG 3 cut(s) 120, 215, 284
SduI GDGCHC 1 cut(s) 584
SetI ASST 7 cut(s) 112, 124, 223, 396, 426, 453, 516
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 1 cut(s) 578
SfcI CTRYAG 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 37, 82
StyD4I CCNGG 3 cut(s) 118, 213, 282
TaaI ACNGT 3 cut(s) 33, 297, 579
TaqI TCGA 2 cut(s) 348, 523
TasI AATT 2 cut(s) 37, 82
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 2 cut(s) 360, 584
TspDTI ATGAA 3 cut(s) 201, 261, 278
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 2 cut(s) 360, 584
Van91I CCANNNNNTGG 1 cut(s) 138
VneI GTGCAC 1 cut(s) 580
XapI RAATTY 1 cut(s) 37
XceI RCATGY 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.