MD05G1045300.v1.1

Annexin repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016740
Physical Location & Seq
Forward (+)
7754439 .. 7757390
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1045300.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGCTACGCTTGTAGTACCGCAACCGGTCCCTCCGGTGGCTGAAGACTGTGAGAAGCTTCATAAAGCTATGCAAGGATGGGGAACAGATGAGAATACTATTATAAACATATTGACTCATAGGAATGGTAAGCAACGTTGTTTGATTCGACAAACTTATGCTGAGAAATATGGAGTGGAGTTATCCAAAGCTCTAGAAGACGAACTTTCCGGGGACTTTCTGAGGGCAATGCTTCTGTGGACACCGCATCCTGCTGAACGTGATGCATGGCTGGCTAATGAAGCGCTAATCTTGAAGGACAACTCAGGCCATCGTCACTACGTAATAATGGAAATCGCTTGTACAAGAACATCCCGAGATCTTTTCGAGGTGAGAGAAGATTACCATGCCCGCTACAAGAGATCTCTCGAAGGAGACGTTGCTTTTCACACAACTGGAGACTTTCGCAAGCTGTTGGTACCTCTTGTGAGTGCTTACAGGTACGAGGGAGCGGAGGTAGATATGACACTGGTGAACCCAGAGGCTACCATACTTCATGAGAAAATCTCTGAGAAGGCATATAATCATGAGGAGATCATCAGAATTCTAACTACAAGGAGCAAGGCGCAACTCAATGCAACTCTTCAACATTATAACGACCAATTTGGCCATCCAATTAACAAGGACCTGAAGAAAAACCCAAAGGATGAATACCTTAGGTTATTACGAGCCAGCATAAAATGCTTGACGTGCTCTGAAAAGTACTTTGAGAAAGCTCTGCGACTGTCAGTGAAAGGCCTTGGAACCACGGAGGAGATTCTGACGAGAGTTATAGTGACTCGAGCAGAGGTCGACATGAAGAAGATAATGGAACAGTACCAGCTCAAGAATGGTGTCTCCTTGGCTCAGGATATTAAGGGTGATACTTCTGGTGACTATTTGGCCACCTTGCTTTCCTTGATTGATCATCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Annexin

Protein Analysis

319

Amino Acids

36.38

Weight (kDa)

6.51

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Annexin PF00191 16 - 79 1.2e-23 Annexin
Annexin PF00191 103 - 155 5.2e-12 Annexin
Annexin PF00191 175 - 236 3.2e-12 Annexin
Annexin PF00191 248 - 313 2.9e-16 Annexin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016740)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26060
malus_domestica MD05G1045300.v1.1
prunus_persica Prupe.8G062100_v2.0.a1 Prupe.8G062100_v2.0.a1
pyrus_communis pycom05g03780
rosa_chinensis RchiOBHm_Chr3g0485531
rosa_laevigata RLG00000023145
rosa_roxburghii Rroxscaffold_6G00396670
rosa_rugosa Rorug03G0219800
rosa_samantha Rh3AG268300 Rh3BG304000 Rh3CG302300 Rh3DG298900
rosa_wichuraiana Rw3G023930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 104, 633
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 1 cut(s) 457
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 831
AciI CCGC 4 cut(s) 20, 245, 391, 491
AclI AACGTT 1 cut(s) 136
AcoI YGGCCR 2 cut(s) 646, 921
AcsI RAATTY 1 cut(s) 582
AcuI CTGAAG 2 cut(s) 63, 689
AfaI GTAC 6 cut(s) 18, 343, 459, 482, 743, 857
AfeI AGCGCT 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 37
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 2 cut(s) 295, 626
AjiI CACGTC 1 cut(s) 729
AluBI AGCT 6 cut(s) 58, 68, 191, 451, 755, 862
AluI AGCT 6 cut(s) 58, 68, 191, 451, 755, 862
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 3 cut(s) 408, 432, 880
Ama87I CYCGRG 2 cut(s) 354, 819
Aor51HI AGCGCT 1 cut(s) 285
AoxI GGCC 4 cut(s) 307, 646, 775, 921
ApoI RAATTY 1 cut(s) 582
AsiGI ACCGGT 1 cut(s) 25
Asp718I GGTACC 1 cut(s) 457
AspLEI GCGC 2 cut(s) 286, 607
AspS9I GGNCC 2 cut(s) 28, 664
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 4 cut(s) 382, 523, 911, 923
AvaI CYCGRG 2 cut(s) 354, 819
AvaII GGWCC 2 cut(s) 28, 664
AxyI CCTNAGG 1 cut(s) 695
BalI TGGCCA 2 cut(s) 648, 923
BanI GGYRCC 1 cut(s) 457
BbsI GAAGAC 2 cut(s) 51, 204
Bbv12I GWGCWC 1 cut(s) 734
BccI CCATC 3 cut(s) 72, 318, 657
BclI TGATCA 1 cut(s) 943
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 408, 432, 880
BfaI CTAG 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 287
BglII AGATCT 2 cut(s) 358, 401
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 1 cut(s) 211
Bme18I GGWCC 2 cut(s) 28, 664
BmeT110I CYCGRG 2 cut(s) 354, 819
BmgBI CACGTC 1 cut(s) 729
BmgT120I GGNCC 2 cut(s) 28, 664
BmiI GGNNCC 3 cut(s) 30, 459, 784
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 2 cut(s) 253, 256
BpiI GAAGAC 2 cut(s) 51, 204
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 848
BpuMI CCSGG 1 cut(s) 211
BsaAI YACGTR 1 cut(s) 322
BsaJI CCNNGG 4 cut(s) 210, 778, 786, 879
BsaWI WCCGGW 2 cut(s) 25, 34
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 439, 513
Bse21I CCTNAGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 4 cut(s) 210, 778, 786, 879
BseGI GGATG 5 cut(s) 83, 247, 350, 649, 691
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 5 cut(s) 153, 212, 318, 540, 899
BseNI ACTGG 2 cut(s) 439, 513
BseRI GAGGAG 2 cut(s) 584, 806
BshFI GGCC 4 cut(s) 309, 648, 777, 923
BshNI GGYRCC 1 cut(s) 457
BshTI ACCGGT 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 734
BsiHKCI CYCGRG 2 cut(s) 354, 819
BsiSI CCGG 3 cut(s) 26, 35, 210
BslFI GGGAC 2 cut(s) 14, 227
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 3 cut(s) 408, 432, 880
BsmBI CGTCTC 1 cut(s) 408
BsmFI GGGAC 2 cut(s) 14, 227
BsnI GGCC 4 cut(s) 309, 648, 777, 923
BsoBI CYCGRG 2 cut(s) 354, 819
Bsp1286I GDGCHC 1 cut(s) 734
Bsp1407I TGTACA 1 cut(s) 341
Bsp143I GATC 4 cut(s) 358, 401, 573, 943
BspACI CCGC 4 cut(s) 20, 245, 391, 491
BspANI GGCC 4 cut(s) 309, 648, 777, 923
BspCNI CTCAG 5 cut(s) 154, 213, 317, 541, 898
BspHI TCATGA 2 cut(s) 535, 565
BspLI GGNNCC 3 cut(s) 30, 459, 784
BspT107I GGYRCC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 341
BsrI ACTGG 2 cut(s) 439, 513
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 4 cut(s) 210, 778, 786, 879
BssMI GATC 4 cut(s) 358, 401, 573, 943
BssT1I CCWWGG 2 cut(s) 778, 879
Bst4CI ACNGT 3 cut(s) 50, 765, 855
Bst6I CTCTTC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 720
BstAUI TGTACA 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 4 cut(s) 273, 391, 449, 712
BstDEI CTNAG 6 cut(s) 162, 221, 304, 549, 695, 885
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 5 cut(s) 83, 247, 350, 649, 691
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 286, 607
BstKTI GATC 4 cut(s) 361, 404, 576, 946
BstMAI GTCTC 3 cut(s) 408, 432, 880
BstMBI GATC 4 cut(s) 358, 401, 573, 943
BstMWI GCNNNNNNNGC 4 cut(s) 272, 281, 720, 729
BstSCI CCNGG 1 cut(s) 209
BstSNI TACGTA 1 cut(s) 322
BstV2I GAAGAC 2 cut(s) 51, 204
BstX2I RGATCY 2 cut(s) 358, 401
BstYI RGATCY 2 cut(s) 358, 401
Bsu36I CCTNAGG 1 cut(s) 695
BsuRI GGCC 4 cut(s) 309, 648, 777, 923
BtgI CCRYGG 1 cut(s) 786
BtrI CACGTC 1 cut(s) 729
BtsCI GGATG 5 cut(s) 83, 247, 350, 649, 691
BtsIMutI CAGTG 2 cut(s) 506, 774
Cac8I GCNNGC 4 cut(s) 273, 391, 449, 712
CciI TCATGA 2 cut(s) 535, 565
CfoI GCGC 2 cut(s) 286, 607
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 28, 664
Csp6I GTAC 6 cut(s) 17, 342, 458, 481, 742, 856
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 7 cut(s) 267, 386, 536, 566, 835, 950, 954
CviQI GTAC 6 cut(s) 17, 342, 458, 481, 742, 856
DdeI CTNAG 6 cut(s) 162, 221, 304, 549, 695, 885
DpnI GATC 4 cut(s) 360, 403, 575, 945
DpnII GATC 4 cut(s) 358, 401, 573, 943
EaeI YGGCCR 2 cut(s) 646, 921
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco105I TACGTA 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 778, 879
Eco147I AGGCCT 1 cut(s) 777
Eco47I GGWCC 2 cut(s) 28, 664
Eco47III AGCGCT 1 cut(s) 285
Eco57I CTGAAG 2 cut(s) 63, 689
Eco81I CCTNAGG 1 cut(s) 695
Eco88I CYCGRG 2 cut(s) 354, 819
EcoO109I RGGNCCY 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 582
EcoT14I CCWWGG 2 cut(s) 778, 879
EcoT22I ATGCAT 2 cut(s) 268, 955
ErhI CCWWGG 2 cut(s) 778, 879
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 7 cut(s) 270, 389, 539, 569, 838, 953, 957
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 2 cut(s) 14, 227
FatI CATG 7 cut(s) 266, 385, 535, 565, 834, 949, 953
FauI CCCGC 1 cut(s) 398
FbaI TGATCA 1 cut(s) 943
FblI GTMKAC 1 cut(s) 831
FokI GGATG 5 cut(s) 90, 234, 337, 636, 698
FspBI CTAG 1 cut(s) 194
GlaI GCGC 2 cut(s) 285, 606
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 4 cut(s) 309, 648, 777, 923
HapII CCGG 3 cut(s) 26, 35, 210
HhaI GCGC 2 cut(s) 286, 607
Hin1II CATG 7 cut(s) 270, 389, 539, 569, 838, 953, 957
Hin6I GCGC 2 cut(s) 284, 605
HinP1I GCGC 2 cut(s) 284, 605
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 115, 145, 796, 817
HpaII CCGG 3 cut(s) 26, 35, 210
HphI GGTGA 4 cut(s) 382, 523, 911, 923
Hpy166II GTNNAC 3 cut(s) 240, 514, 832
Hpy188I TCNGA 5 cut(s) 222, 550, 581, 736, 801
Hpy188III TCNNGA 8 cut(s) 194, 292, 354, 407, 536, 566, 865, 887
Hpy8I GTNNAC 3 cut(s) 240, 514, 832
HpyAV CCTTC 3 cut(s) 289, 404, 547
HpyCH4III ACNGT 3 cut(s) 50, 765, 855
HpyCH4IV ACGT 5 cut(s) 136, 259, 321, 417, 728
HpyCH4V TGCA 4 cut(s) 73, 266, 617, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 281, 720, 729
HpyF3I CTNAG 6 cut(s) 162, 221, 304, 549, 695, 885
HpySE526I ACGT 5 cut(s) 136, 259, 321, 417, 728
Hsp92II CATG 7 cut(s) 270, 389, 539, 569, 838, 953, 957
HspAI GCGC 2 cut(s) 284, 605
KpnI GGTACC 1 cut(s) 461
Ksp22I TGATCA 1 cut(s) 943
Kzo9I GATC 4 cut(s) 358, 401, 573, 943
LmnI GCTCC 2 cut(s) 488, 597
LweI GCATC 2 cut(s) 253, 256
MaeI CTAG 1 cut(s) 194
MaeII ACGT 5 cut(s) 136, 259, 321, 417, 728
MaeIII GTNAC 3 cut(s) 314, 814, 911
MalI GATC 4 cut(s) 360, 403, 575, 945
MbiI CCGCTC 1 cut(s) 491
MboI GATC 4 cut(s) 358, 401, 573, 943
MboII GAAGA 7 cut(s) 56, 209, 389, 614, 682, 850, 853
MflI RGATCY 2 cut(s) 358, 401
MhlI GDGCHC 1 cut(s) 734
MlsI TGGCCA 2 cut(s) 648, 923
MluCI AATT 3 cut(s) 582, 641, 654
MluNI TGGCCA 2 cut(s) 648, 923
MlyI GAGTC 2 cut(s) 109, 811
Mox20I TGGCCA 2 cut(s) 648, 923
Mph1103I ATGCAT 2 cut(s) 268, 955
MscI TGGCCA 2 cut(s) 648, 923
MseI TTAA 2 cut(s) 657, 894
MslI CAYNNNNRTG 1 cut(s) 123
Msp20I TGGCCA 2 cut(s) 648, 923
MspI CCGG 3 cut(s) 26, 35, 210
MspR9I CCNGG 1 cut(s) 211
MwoI GCNNNNNNNGC 4 cut(s) 272, 281, 720, 729
NciI CCSGG 1 cut(s) 211
NdeII GATC 4 cut(s) 358, 401, 573, 943
NlaIII CATG 7 cut(s) 270, 389, 539, 569, 838, 953, 957
NlaIV GGNNCC 3 cut(s) 30, 459, 784
NmuCI GTSAC 3 cut(s) 314, 814, 911
NsiI ATGCAT 2 cut(s) 268, 955
PaeR7I CTCGAG 1 cut(s) 819
PagI TCATGA 2 cut(s) 535, 565
PceI AGGCCT 1 cut(s) 777
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 2 cut(s) 145, 796
PinAI ACCGGT 1 cut(s) 25
PleI GAGTC 2 cut(s) 109, 811
PpsI GAGTC 2 cut(s) 109, 811
Ppu21I YACGTR 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 664
PsiI TTATAA 2 cut(s) 104, 633
Psp1406I AACGTT 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 664
PspN4I GGNNCC 3 cut(s) 30, 459, 784
PspPI GGNCC 2 cut(s) 28, 664
PspPPI RGGWCCY 1 cut(s) 664
PspXI VCTCGAGB 1 cut(s) 819
PsuI RGATCY 2 cut(s) 358, 401
RsaI GTAC 6 cut(s) 18, 343, 459, 482, 743, 857
RsaNI GTAC 6 cut(s) 17, 342, 458, 481, 742, 856
RseI CAYNNNNRTG 1 cut(s) 123
SalI GTCGAC 1 cut(s) 830
SaqAI TTAA 2 cut(s) 657, 894
Sau3AI GATC 4 cut(s) 358, 401, 573, 943
Sau96I GGNCC 2 cut(s) 28, 664
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 2 cut(s) 109, 811
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 1 cut(s) 734
SfaNI GCATC 2 cut(s) 253, 256
Sfr274I CTCGAG 1 cut(s) 819
SinI GGWCC 2 cut(s) 28, 664
SlaI CTCGAG 1 cut(s) 819
SmiMI CAYNNNNRTG 1 cut(s) 123
SmlI CTYRAG 2 cut(s) 819, 863
SmoI CTYRAG 2 cut(s) 819, 863
SnaBI TACGTA 1 cut(s) 322
Sse9I AATT 3 cut(s) 582, 641, 654
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 4 cut(s) 20, 245, 391, 491
SspMI CTAG 1 cut(s) 194
StuI AGGCCT 1 cut(s) 777
StyD4I CCNGG 1 cut(s) 209
StyI CCWWGG 2 cut(s) 778, 879
TaaI ACNGT 3 cut(s) 50, 765, 855
TaiI ACGT 5 cut(s) 139, 262, 324, 420, 731
TaqI TCGA 5 cut(s) 148, 366, 408, 820, 831
TasI AATT 3 cut(s) 582, 641, 654
TatI WGTACW 2 cut(s) 341, 741
TfiI GAWTC 2 cut(s) 145, 796
Tru1I TTAA 2 cut(s) 657, 894
Tru9I TTAA 2 cut(s) 657, 894
TscAI CASTG 2 cut(s) 513, 774
TseFI GTSAC 3 cut(s) 314, 814, 911
Tsp45I GTSAC 3 cut(s) 314, 814, 911
TspDTI ATGAA 5 cut(s) 50, 294, 524, 702, 851
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 2 cut(s) 513, 774
VpaK11BI GGWCC 2 cut(s) 28, 664
XapI RAATTY 1 cut(s) 582
XbaI TCTAGA 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 866
XhoI CTCGAG 1 cut(s) 819
XmiI GTMKAC 1 cut(s) 831
XspI CTAG 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 2 cut(s) 268, 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.