MD05G1079700.v1.1

DNA repair and recombination protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008727
Physical Location & Seq
Reverse (-)
16732196 .. 16740328
8133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1079700.v1.1.491

Sequence Viewer

Length: 2853 bp
ATGGAGGACGAAGACGAAGTTCTCTCAATCTCCGACGCCTCCGGTTCATCCGATTCCTCCTCCGAATCCAGCGAGGGGTACTCGGCTGACCAAGAAGAGCTAGACCAAGAAGAAGAAGAAGACCGCCAAAACGACTTCGTGGTGGTCGAGAATAGTCGAAAACCCATAGACCCTCCTCGGCCCACCGCCGAAGATCAGAAATTGAAAAACGTCGATGCTCTCGTCAGAGGTAACCTTGTCGTGAAAAGGCAGCCACTGCTTCCTCGAGTTCTCTCAGTGACGGAAGGAGCAGCAGTTTGTAGGAAACCCTTTAAACCTCCATGCTCTATTGGCTATGAGGATGGAACTGACCAGCTCGTGCGACGTCTCAGTGCACGCAGACGATTTGTTCCCTGGGGCTCATCAACTGCAGCATTCATTCCACATATTAATATGAATTTGAGTATAGAAAATGAGGCTGAGGAAGATCCAGTGGAAAAGAGTGTGACACTACCTACCGGAATTGATCCTTTGGTCTTGTGGCAATCTGAAGAATTTGAAATTGGGGCTCCTAATATGGTGCAGATAGTAGTGGACCCAGTGCTTGTTCGTTTCCTTCGACCACATCAAAGAGAAGGCGTTCAGTTTATGTTTGAATGTGTTTCAGGGTTAAATAGTGCATCAAATATATATGGATGCATTCTGGCAGATGATATGGGTTTGGGAAAGACATTGCAGTCTATCACATTACTATATACTCTTCTATGTCAAGGGTTTGATGGGAAACCAATGGTTAAAAAGGCCATTATAGTCACCCCTACCAGTCTTGTGAGCAACTGGGAGGCTGAGATAAAGAAGTGGGTTGGAGAAAGAGTTAATCTTATTGCCCTTTGTGAAAGTACCAGGGATGATGTTGTTTGTGGAATTGATCGATTCACAAGTCCTTGCGGCCGTGTACAGGTACTGATTGTTTCATACGAGACATTTCGGATGCATTCTGCAAAATTTAGCCAAAGCGAGTCCTGTGACCTTCTCATATGTGATGAGGCTCACCGTCTGAAGAATGATCAGACAATGACGAATCGGGCTTTGGCTGCTCTTTCATGTAAGCGGCGTATTCTATTGTCGGGAACTCCCATGCAGAATGACCTAGAAGAGTTCTTTGCCATGGTCAATTTTACTAATCCGGGAATCTTAGGTGATGCTGTATATTTTCGCCGCTACTATGAGGCACCTATTATTTGTGGAAGAGAACCTAATGCCTCTGAAGAAGAGAGGAAGCTAAGTGTTGAGCGATCTGCTGAACTAAGTGGAAAAGTTAATCAGTTTATATTGCGGAGGACTAATGCACTACTATCAAATCACCTCCCACCAAAGATAATTGAAGTTGTTTGTTGCAAGTTGACTCCTCTTCAAATAGACATATACAAACATTTCATACATTCCAAAAATGTTAAACGAGCAATTTCTGAAGACACGAAGCAATCAAAGATTTTGGCTTATATTACAGCTCTTAAGAAGCTATGCAATCATCCAAAGCTCATCTATGATACTATAAGAAGTGGAAGTCCAGGAACTTCAGGTTTTGAGGACTGTATTCGCTTTTTCCCTCCAGATATGTTCTCTGGAAGATCTGGATCATGGACTGGCGGGGATGGGGCTTGGGTTGAACTGTCTGGAAAAATGCATGTTTTAGCTCGGTTACTGGCCCATTTACGTAAGAAGACTGATGACCGTATTGTCCTTGTCTCAAACTATACGCAGACACTGGACCTTTTTGCGCAATTGTGTCGAGAAAGAAGATACCCATATTTGAGGCTTGACGGAACCACATCTATCAGTAAAAGACAGAAATTAGTAAACCATTTCAATGACCCCTCAAAGGATGAATTTGTATTTCTCTTAAGCAGCAAGGCTGGTGGTTGTGGTCTTAATTTAATAGGTGGAAATCGGCTAGTTTTGTTTGATCCTGACTGGAATCCTGCCAACGATAAACAAGCTGCTGCAAGAGTTTGGAGGGATGGGCAAAAGAAGAGAGTATACATTTACAGATTTTTGAGTACTGGAACAATTGAAGAAAAGGTGTACCAGCGCCAGATGTCAAAAGAAGGGCTTCAAAAGGTTATTCAGCACGAACAAACAGATAACCCAAAGGCACAGGGAAACCTTCTTTCAACAGAAGACCTGCGCGATCTATTCACATTTCATGAGAATGTGAGGTCTGACGTGCATGAGAAGATGAACTGCATCCGTTGCCAAAATACCAATGATACACCTCAGACCACAGCCGAGGGAGATGCAAACGAATCAACCAATCAAAATGGTCAGTCTGGGCAAGTAATATCTGATATAGGCAGATTTGCAGAACATGCTGGATGCTTCCATGAATTAAAGAGCTCGGAGAAGCAGGTAGGGACACCTCTAGAAGAAGATCTAAGTAATTGGGGGCACCATTTCTCCCCAACTTCAGTACCTGATTCTATTCTCCAAGCTTCAGCTGGAGATGAGGTCACATTTGTGTTTACAAACCAAGTGGATGGGAAGCTTGTACCAATTGAATCAAAAGTAAGGCCAAAGATTCAGGAACAAGAAGGAAAAGAGAACCGTCCCAGATTAAAACAAAACCTGAACCAGAAATCCATGCCATTGTCTTGGCAAAGAAAACCGCTCGAATCTGTTTCTTTGGGTGAAAATTCTATCAGAAGCACATCTGCCCCTTTCAAGCCCTCAGAAAAGTCCACCGTGAAATCAGTAAAAACTTCTTTGAAGGGTTCAGTGCATGTTGCATTAACGCCTGAACTGTCTCTTAAAAATCGATTGCCCCAGAAAAGATCGTCTCCTGATTCTGTGGAACACGACGACGATTTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000280 GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0033170 GO:0033554 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045003 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051701 GO:0051704 GO:0051716 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070887 GO:0071214 GO:0071241 GO:0071248 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0104004 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

951

Amino Acids

106.6

Weight (kDa)

6.13

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 199 - 372 9.8e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 202 - 507 2.7e-69 SNF2-related domain
DEAD PF00270 227 - 379 1.4e-07 DEAD/DEAH box helicase
Helicase_C PF00271 554 - 668 3.5e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 715, 1718
AatII GACGTC 1 cut(s) 367
Acc16I TGCGCA 1 cut(s) 1761
Acc36I ACCTGC 2 cut(s) 2172, 2377
AccB1I GGYRCC 2 cut(s) 1210, 2427
AccBSI CCGCTC 1 cut(s) 2647
AccI GTMKAC 1 cut(s) 2019
AccII CGCG 1 cut(s) 2169
AciI CCGC 8 cut(s) 124, 186, 927, 1090, 1198, 1315, 1629, 2645
AclWI GGATC 4 cut(s) 461, 500, 1624, 1940
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 5 cut(s) 436, 533, 983, 1868, 2671
AcuI CTGAAG 7 cut(s) 549, 1058, 1266, 1470, 1542, 2430, 2457
AcyI GRCGYC 2 cut(s) 36, 364
AfaI GTAC 8 cut(s) 80, 880, 936, 942, 2041, 2066, 2451, 2529
AfiI CCNNNNNNNGG 3 cut(s) 75, 937, 1861
AflII CTTAAG 2 cut(s) 1493, 1882
AjiI CACGTC 1 cut(s) 2206
AjnI CCWGG 3 cut(s) 392, 881, 1549
AjuI GAANNNNNNNTTGG 4 cut(s) 525, 557, 1052, 1084
AleI CACNNNNGTG 1 cut(s) 2495
AloI GAACNNNNNNTCC 2 cut(s) 2600, 2632
Alw21I GWGCWC 2 cut(s) 376, 2378
Alw26I GTCTC 5 cut(s) 371, 953, 1732, 2787, 2820
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 4 cut(s) 461, 500, 1624, 1940
AlwNI CAGNNNCTG 4 cut(s) 256, 943, 1747, 2453
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 5 cut(s) 179, 780, 928, 1686, 2549
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 7 cut(s) 250, 290, 410, 1073, 1887, 1979, 1982
ApoI RAATTY 5 cut(s) 436, 533, 983, 1868, 2671
ArsI GACNNNNNNTTYG 1 cut(s) 2828
AseI ATTAAT 1 cut(s) 429
Asp700I GAANNNNTTC 2 cut(s) 618, 2091
AspLEI GCGC 3 cut(s) 1762, 2073, 2169
AspS9I GGNCC 4 cut(s) 180, 574, 1687, 1750
AsuC2I CCSGG 1 cut(s) 1167
AsuHPI GGTGA 5 cut(s) 784, 1022, 1190, 1334, 2678
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 2 cut(s) 574, 1750
BaeGI GKGCMC 2 cut(s) 376, 2430
BaeI ACNNNNGTAYC 2 cut(s) 2433, 2466
BanI GGYRCC 2 cut(s) 1210, 2427
BanII GRGCYC 3 cut(s) 401, 550, 2378
BarI GAAGNNNNNNTAC 2 cut(s) 2003, 2035
BauI CACGAG 1 cut(s) 356
BbsI GAAGAC 5 cut(s) 18, 126, 1458, 1709, 2166
Bbv12I GWGCWC 2 cut(s) 376, 2378
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 7 cut(s) 262, 302, 422, 1060, 1899, 1966, 1969
BccI CCATC 5 cut(s) 335, 752, 1628, 1994, 2510
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 4 cut(s) 1751, 1785, 2258, 2292
BciT130I CCWGG 3 cut(s) 394, 883, 1551
BclI TGATCA 1 cut(s) 1045
BcnI CCSGG 1 cut(s) 1167
BcoDI GTCTC 5 cut(s) 371, 953, 1732, 2787, 2820
BfaI CTAG 4 cut(s) 101, 1130, 1934, 2402
BfmI CTRYAG 1 cut(s) 408
BfoI RGCGCY 1 cut(s) 2074
BfrI CTTAAG 2 cut(s) 1493, 1882
BfuAI ACCTGC 2 cut(s) 2172, 2377
BglII AGATCT 2 cut(s) 1610, 2410
BmcAI AGTACT 1 cut(s) 2041
Bme1390I CCNGG 4 cut(s) 394, 883, 1167, 1551
Bme18I GGWCC 2 cut(s) 574, 1750
BmeT110I CYCGRG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 2206
BmgT120I GGNCC 4 cut(s) 180, 574, 1687, 1750
BmiI GGNNCC 5 cut(s) 549, 576, 1212, 1807, 2429
BmrFI CCNGG 4 cut(s) 394, 883, 1167, 1551
BmrI ACTGGG 2 cut(s) 572, 826
BmsI GCATC 8 cut(s) 205, 665, 668, 960, 1171, 2235, 2266, 2345
BmuI ACTGGG 2 cut(s) 572, 826
BpiI GAAGAC 5 cut(s) 18, 126, 1458, 1709, 2166
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 2 cut(s) 1575, 2499
Bpu10I CCTNAGC 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 1167
Bsa29I ATCGAT 2 cut(s) 910, 2794
BsaAI YACGTR 1 cut(s) 1697
BsaBI GATNNNNATC 1 cut(s) 1615
BsaHI GRCGYC 2 cut(s) 36, 364
BsaJI CCNNGG 6 cut(s) 176, 392, 393, 882, 1146, 2268
BsaWI WCCGGW 2 cut(s) 41, 497
BsaXI ACNNNNNCTCC 8 cut(s) 279, 309, 837, 867, 2420, 2450, 2472, 2502
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 937, 1861
Bse1I ACTGG 9 cut(s) 470, 578, 801, 821, 1630, 1689, 1752, 1958, 2047
Bse3DI GCAATG 1 cut(s) 710
Bse8I GATNNNNATC 1 cut(s) 1615
BseBI CCWGG 3 cut(s) 394, 883, 1551
BseCI ATCGAT 2 cut(s) 910, 2794
BseDI CCNNGG 6 cut(s) 176, 392, 393, 882, 1146, 2268
BseJI GATNNNNATC 1 cut(s) 1615
BseLI CCNNNNNNNGG 3 cut(s) 75, 937, 1861
BseMI GCAATG 1 cut(s) 710
BseMII CTCAG 6 cut(s) 288, 382, 450, 816, 2270, 2721
BseNI ACTGG 9 cut(s) 470, 578, 801, 821, 1630, 1689, 1752, 1958, 2047
BseRI GAGGAG 3 cut(s) 49, 165, 1377
BseSI GKGCMC 2 cut(s) 376, 2430
BseX3I CGGCCG 1 cut(s) 928
BseXI GCAGC 7 cut(s) 262, 302, 422, 1060, 1899, 1966, 1969
BsgI GTGCAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 2169
Bsh1285I CGRYCG 1 cut(s) 931
BshFI GGCC 5 cut(s) 181, 782, 930, 1688, 2551
BshNI GGYRCC 2 cut(s) 1210, 2427
BshVI ATCGAT 2 cut(s) 910, 2794
BsiEI CGRYCG 1 cut(s) 931
BsiHKAI GWGCWC 2 cut(s) 376, 2378
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 3 cut(s) 42, 498, 1166
BslFI GGGAC 2 cut(s) 2407, 2571
BslI CCNNNNNNNGG 3 cut(s) 75, 937, 1861
BsmAI GTCTC 5 cut(s) 371, 953, 1732, 2787, 2820
BsmBI CGTCTC 2 cut(s) 371, 2820
BsmFI GGGAC 2 cut(s) 2407, 2571
BsmI GAATGC 3 cut(s) 413, 678, 973
BsnI GGCC 5 cut(s) 181, 782, 930, 1688, 2551
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 5 cut(s) 376, 401, 550, 2378, 2430
Bsp1407I TGTACA 1 cut(s) 934
Bsp19I CCATGG 1 cut(s) 1146
BspACI CCGC 8 cut(s) 124, 186, 927, 1090, 1198, 1315, 1629, 2645
BspANI GGCC 5 cut(s) 181, 782, 930, 1688, 2551
BspCNI CTCAG 6 cut(s) 287, 381, 451, 817, 2269, 2720
BspDI ATCGAT 2 cut(s) 910, 2794
BspFNI CGCG 1 cut(s) 2169
BspHI TCATGA 1 cut(s) 2185
BspLI GGNNCC 5 cut(s) 549, 576, 1212, 1807, 2429
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 2 cut(s) 2172, 2377
BspPI GGATC 4 cut(s) 461, 500, 1624, 1940
BspQI GCTCTTC 1 cut(s) 90
BspT107I GGYRCC 2 cut(s) 1210, 2427
BspTI CTTAAG 2 cut(s) 1493, 1882
BsrBI CCGCTC 1 cut(s) 2647
BsrDI GCAATG 1 cut(s) 710
BsrGI TGTACA 1 cut(s) 934
BsrI ACTGG 9 cut(s) 470, 578, 801, 821, 1630, 1689, 1752, 1958, 2047
BssECI CCNNGG 6 cut(s) 176, 392, 393, 882, 1146, 2268
BssNAI GTATAC 1 cut(s) 2020
BssNI GRCGYC 2 cut(s) 36, 364
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 1146
Bst1107I GTATAC 1 cut(s) 2020
Bst2BI CACGAG 1 cut(s) 356
Bst2UI CCWGG 3 cut(s) 394, 883, 1551
Bst4CI ACNGT 7 cut(s) 1034, 1574, 1653, 1715, 2585, 2722, 2781
Bst6I CTCTTC 7 cut(s) 90, 744, 1128, 1222, 1245, 1395, 2006
BstACI GRCGYC 2 cut(s) 36, 364
BstAFI CTTAAG 2 cut(s) 1493, 1882
BstAPI GCANNNNNTGC 3 cut(s) 256, 2232, 2348
BstAUI TGTACA 1 cut(s) 934
BstBAI YACGTR 1 cut(s) 1697
BstC8I GCNNGC 1 cut(s) 376
BstDSI CCRYGG 1 cut(s) 1146
BstEII GGTNACC 1 cut(s) 230
BstFNI CGCG 1 cut(s) 2169
BstH2I RGCGCY 1 cut(s) 2074
BstHHI GCGC 3 cut(s) 1762, 2073, 2169
BstMAI GTCTC 5 cut(s) 371, 953, 1732, 2787, 2820
BstMCI CGRYCG 1 cut(s) 931
BstMWI GCNNNNNNNGC 5 cut(s) 256, 330, 1073, 2232, 2348
BstNI CCWGG 3 cut(s) 394, 883, 1551
BstNSI RCATGY 3 cut(s) 1670, 2351, 2762
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 392, 881, 1165, 1549
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 2 cut(s) 376, 2430
BstSNI TACGTA 1 cut(s) 1697
BstUI CGCG 1 cut(s) 2169
BstV1I GCAGC 7 cut(s) 262, 302, 422, 1060, 1899, 1966, 1969
BstV2I GAAGAC 5 cut(s) 18, 126, 1458, 1709, 2166
BstX2I RGATCY 3 cut(s) 466, 1610, 2410
BstXI CCANNNNNNTGG 2 cut(s) 2516, 2631
BstYI RGATCY 3 cut(s) 466, 1610, 2410
BstZ17I GTATAC 1 cut(s) 2020
BstZI CGGCCG 1 cut(s) 928
Bsu15I ATCGAT 2 cut(s) 910, 2794
BsuRI GGCC 5 cut(s) 181, 782, 930, 1688, 2551
BsuTUI ATCGAT 2 cut(s) 910, 2794
BtgI CCRYGG 1 cut(s) 1146
BtrI CACGTC 1 cut(s) 2206
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 7 cut(s) 254, 282, 376, 477, 585, 1745, 2760
BveI ACCTGC 2 cut(s) 2172, 2377
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 4 cut(s) 256, 943, 1747, 2453
CciI TCATGA 1 cut(s) 2185
CfoI GCGC 3 cut(s) 1762, 2073, 2169
Cfr13I GGNCC 4 cut(s) 180, 574, 1687, 1750
ClaI ATCGAT 2 cut(s) 910, 2794
CseI GACGC 1 cut(s) 44
Csp6I GTAC 8 cut(s) 79, 879, 935, 941, 2040, 2065, 2450, 2528
CspCI CAANNNNNGTGG 4 cut(s) 1879, 1914, 2493, 2528
CviQI GTAC 8 cut(s) 79, 879, 935, 941, 2040, 2065, 2450, 2528
DraI TTTAAA 1 cut(s) 313
DrdI GACNNNNNNGTC 2 cut(s) 715, 1718
DseDI GACNNNNNNGTC 2 cut(s) 715, 1718
EaeI YGGCCR 1 cut(s) 928
EagI CGGCCG 1 cut(s) 928
Eam1104I CTCTTC 7 cut(s) 90, 744, 1128, 1222, 1245, 1395, 2006
EarI CTCTTC 7 cut(s) 90, 744, 1128, 1222, 1245, 1395, 2006
Ecl136II GAGCTC 1 cut(s) 2376
EclXI CGGCCG 1 cut(s) 928
Eco105I TACGTA 1 cut(s) 1697
Eco130I CCWWGG 1 cut(s) 1146
Eco24I GRGCYC 3 cut(s) 401, 550, 2378
Eco47I GGWCC 2 cut(s) 574, 1750
Eco52I CGGCCG 1 cut(s) 928
Eco53kI GAGCTC 1 cut(s) 2376
Eco57I CTGAAG 7 cut(s) 549, 1058, 1266, 1470, 1542, 2430, 2457
Eco88I CYCGRG 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 2376
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 392, 881, 1549
EcoT14I CCWWGG 1 cut(s) 1146
EcoT22I ATGCAT 3 cut(s) 680, 975, 1668
EcoT38I GRGCYC 3 cut(s) 401, 550, 2378
ErhI CCWWGG 1 cut(s) 1146
Esp3I CGTCTC 2 cut(s) 371, 2820
FalI AAGNNNNNCTT 2 cut(s) 2076, 2108
FaqI GGGAC 2 cut(s) 2407, 2571
FauI CCCGC 1 cut(s) 1622
FauNDI CATATG 1 cut(s) 1016
FbaI TGATCA 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 2019
FriOI GRGCYC 3 cut(s) 401, 550, 2378
FspBI CTAG 4 cut(s) 101, 1130, 1934, 2402
FspI TGCGCA 1 cut(s) 1761
GlaI GCGC 3 cut(s) 1761, 2072, 2168
GsuI CTGGAG 2 cut(s) 1575, 2499
HaeII RGCGCY 1 cut(s) 2074
HaeIII GGCC 5 cut(s) 181, 782, 930, 1688, 2551
HapII CCGG 3 cut(s) 42, 498, 1166
HgaI GACGC 1 cut(s) 44
HhaI GCGC 3 cut(s) 1762, 2073, 2169
Hin1I GRCGYC 2 cut(s) 36, 364
Hin6I GCGC 3 cut(s) 1760, 2071, 2167
HinP1I GCGC 3 cut(s) 1760, 2071, 2167
HincII GTYRAC 1 cut(s) 1383
HindII GTYRAC 1 cut(s) 1383
HindIII AAGCTT 2 cut(s) 2469, 2522
HpaII CCGG 3 cut(s) 42, 498, 1166
HphI GGTGA 5 cut(s) 784, 1022, 1190, 1334, 2678
Hpy166II GTNNAC 9 cut(s) 374, 574, 935, 1383, 1840, 2020, 2065, 2502, 2718
Hpy8I GTNNAC 9 cut(s) 374, 574, 935, 1383, 1840, 2020, 2065, 2502, 2718
Hpy99I CGWCG 5 cut(s) 38, 215, 366, 2840, 2843
HpyAV CCTTC 8 cut(s) 278, 605, 608, 1019, 2081, 2156, 2564, 2740
HpyCH4III ACNGT 7 cut(s) 1034, 1574, 1653, 1715, 2585, 2722, 2781
HpyCH4IV ACGT 4 cut(s) 210, 364, 1696, 2205
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 330, 1073, 2232, 2348
HpySE526I ACGT 4 cut(s) 210, 364, 1696, 2205
Hsp92I GRCGYC 2 cut(s) 36, 364
HspAI GCGC 3 cut(s) 1760, 2071, 2167
Ksp22I TGATCA 1 cut(s) 1045
LguI GCTCTTC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 287, 553
Lsp1109I GCAGC 7 cut(s) 262, 302, 422, 1060, 1899, 1966, 1969
LweI GCATC 8 cut(s) 205, 665, 668, 960, 1171, 2235, 2266, 2345
MaeI CTAG 4 cut(s) 101, 1130, 1934, 2402
MaeII ACGT 4 cut(s) 210, 364, 1696, 2205
MaeIII GTNAC 7 cut(s) 230, 277, 484, 790, 1004, 1680, 2488
MbiI CCGCTC 1 cut(s) 2647
MfeI CAATTG 3 cut(s) 1763, 2049, 2532
MflI RGATCY 3 cut(s) 466, 1610, 2410
MhlI GDGCHC 5 cut(s) 376, 401, 550, 2378, 2430
MlyI GAGTC 2 cut(s) 1007, 1378
MmeI TCCRAC 2 cut(s) 57, 823
Mph1103I ATGCAT 3 cut(s) 680, 975, 1668
MroXI GAANNNNTTC 2 cut(s) 618, 2091
MslI CAYNNNNRTG 3 cut(s) 1848, 2190, 2495
MspA1I CMGCKG 1 cut(s) 2477
MspCI CTTAAG 2 cut(s) 1493, 1882
MspI CCGG 3 cut(s) 42, 498, 1166
MspR9I CCNGG 4 cut(s) 394, 883, 1167, 1551
MunI CAATTG 3 cut(s) 1763, 2049, 2532
Mva1269I GAATGC 3 cut(s) 413, 678, 973
MvaI CCWGG 3 cut(s) 394, 883, 1551
MvnI CGCG 1 cut(s) 2169
MwoI GCNNNNNNNGC 5 cut(s) 256, 330, 1073, 2232, 2348
NciI CCSGG 1 cut(s) 1167
NcoI CCATGG 1 cut(s) 1146
NdeI CATATG 1 cut(s) 1016
NlaIV GGNNCC 5 cut(s) 549, 576, 1212, 1807, 2429
NmeAIII GCCGAG 3 cut(s) 62, 157, 2293
NmuCI GTSAC 5 cut(s) 277, 484, 790, 1004, 2488
NsbI TGCGCA 1 cut(s) 1761
NsiI ATGCAT 3 cut(s) 680, 975, 1668
NspI RCATGY 3 cut(s) 1670, 2351, 2762
OliI CACNNNNGTG 1 cut(s) 2495
PaeR7I CTCGAG 1 cut(s) 264
PagI TCATGA 1 cut(s) 2185
PasI CCCWGGG 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 90
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 219, 595
PctI GAATGC 3 cut(s) 413, 678, 973
PdmI GAANNNNTTC 2 cut(s) 618, 2091
PfoI TCCNGGA 2 cut(s) 1165, 1549
PleI GAGTC 2 cut(s) 1006, 1378
PpsI GAGTC 2 cut(s) 1006, 1378
Ppu21I YACGTR 1 cut(s) 1697
PshBI ATTAAT 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 2378
Psp6I CCWGG 3 cut(s) 392, 881, 1549
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 3 cut(s) 392, 881, 1549
PspN4I GGNNCC 5 cut(s) 549, 576, 1212, 1807, 2429
PspPI GGNCC 4 cut(s) 180, 574, 1687, 1750
PspXI VCTCGAGB 1 cut(s) 264
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 4 cut(s) 256, 943, 1747, 2453
PsuI RGATCY 3 cut(s) 466, 1610, 2410
PvuII CAGCTG 1 cut(s) 2477
RsaI GTAC 8 cut(s) 80, 880, 936, 942, 2041, 2066, 2451, 2529
RsaNI GTAC 8 cut(s) 79, 879, 935, 941, 2040, 2065, 2450, 2528
RseI CAYNNNNRTG 3 cut(s) 1848, 2190, 2495
SacI GAGCTC 1 cut(s) 2378
SapI GCTCTTC 1 cut(s) 90
Sau96I GGNCC 4 cut(s) 180, 574, 1687, 1750
ScaI AGTACT 1 cut(s) 2041
SchI GAGTC 2 cut(s) 1007, 1378
ScrFI CCNGG 4 cut(s) 394, 883, 1167, 1551
SduI GDGCHC 5 cut(s) 376, 401, 550, 2378, 2430
SfaNI GCATC 8 cut(s) 205, 665, 668, 960, 1171, 2235, 2266, 2345
SfcI CTRYAG 1 cut(s) 408
Sfr274I CTCGAG 1 cut(s) 264
SinI GGWCC 2 cut(s) 574, 1750
SlaI CTCGAG 1 cut(s) 264
SmiMI CAYNNNNRTG 3 cut(s) 1848, 2190, 2495
SmlI CTYRAG 3 cut(s) 264, 1493, 1882
SmoI CTYRAG 3 cut(s) 264, 1493, 1882
SnaBI TACGTA 1 cut(s) 1697
SsiI CCGC 8 cut(s) 124, 186, 927, 1090, 1198, 1315, 1629, 2645
SspMI CTAG 4 cut(s) 101, 1130, 1934, 2402
SstI GAGCTC 1 cut(s) 2378
StyD4I CCNGG 4 cut(s) 392, 881, 1165, 1549
StyI CCWWGG 1 cut(s) 1146
TaaI ACNGT 7 cut(s) 1034, 1574, 1653, 1715, 2585, 2722, 2781
TaiI ACGT 4 cut(s) 213, 367, 1699, 2208
TaqI TCGA 9 cut(s) 147, 157, 213, 265, 598, 910, 1771, 2649, 2794
TatI WGTACW 2 cut(s) 934, 2039
TauI GCSGC 3 cut(s) 930, 1093, 1200
TscAI CASTG 7 cut(s) 261, 282, 376, 477, 585, 1752, 2760
TseFI GTSAC 5 cut(s) 277, 484, 790, 1004, 2488
TseI GCWGC 7 cut(s) 250, 290, 410, 1073, 1887, 1979, 1982
Tsp45I GTSAC 5 cut(s) 277, 484, 790, 1004, 2488
TspGWI ACGGA 3 cut(s) 296, 1818, 2219
TspRI CASTG 7 cut(s) 261, 282, 376, 477, 585, 1752, 2760
Vha464I CTTAAG 2 cut(s) 1493, 1882
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 2 cut(s) 574, 1750
VspI ATTAAT 1 cut(s) 429
XapI RAATTY 5 cut(s) 436, 533, 983, 1868, 2671
XbaI TCTAGA 1 cut(s) 2401
XceI RCATGY 3 cut(s) 1670, 2351, 2762
XcmI CCANNNNNNNNNTGG 1 cut(s) 2474
XhoI CTCGAG 1 cut(s) 264
XmiI GTMKAC 1 cut(s) 2019
XmnI GAANNNNTTC 2 cut(s) 618, 2091
XspI CTAG 4 cut(s) 101, 1130, 1934, 2402
ZraI GACGTC 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 2041
Zsp2I ATGCAT 3 cut(s) 680, 975, 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.