Rroxscaffold_7G00208400

DNA repair and recombination protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008727
Physical Location & Seq
Reverse (-)
58422360 .. 58432023
9664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208400.1

Sequence Viewer

Length: 2889 bp
ATGGAAGGCGAAGGAGAAGTCATCTCACTCTCCGATTCCTCCTCCGATTCCAGTTCCAGCGACGACTACACTGCCGACCCACGAGACTTGGATCTCGGAGAGGAAGAAGAAGAAGAAGAAGACGAAGGAGACCAAGTGCTCGAAAACGAACCAGAGGTTCAAAACGACGACGTACGCGAGAATCGTCGGAGATCCAAGGCCCGCCCTCCGCTTCCGCCGCCGTCCGGAAAAGATCGGAAATCGAAGAATGTCGATGCTCTCCTAAGCGGCAACCTTGTGGTTAAAAGACAGCCTCTTCTTCCACGAGTTCTGTCGGTGACGGAAGGAGCAACAGTTTGTAGGAAACCCTTTAAACCACCATGCTCCAAAGGCTATGATGATGGAAATGACCAGCTCGCTCGGCGTCTCTCAGCACGCAGACGGTTTGTTCCCTGGGGCGGTTCATCAAGTGCACCGTTTTTTCCGGTTATTGAGAGGTTTCCTAGTTTGCCAAATGATGCTGAGAAAGATGTAGTGGAAGAGAGTGTGACCCTGCCACCTGGAATTGATCCTTTGGTCTTGTGGCAACCTGAAGAATTCGAGGATGGGGATGCTAATATGGTGCAGATAGTAGTGGACCCTCTGCTTGTTCGTTTCCTTCGACCACATCAAAGAGAAGGGGTTCAATTCATGTTTGATTGTGTTGCGGGCATAAATAGTGCTGCAAATATACATGGATGTATTCTTGCAGATGATATGGGGTTGGGAAAGACATTGCAGTCAATCACATTACTTTATACCCTTCTTTACCAAGGGTTTGATGGGAAATCAATGGTTAAAAAGGCCATTATAGTCACTCCCACCAGTCTTGTGAGCAATTGGGAGGCTGAGATAAAGAAGTGGGTCGGAGACAGAGTTTGCCTCATTGCTCTGTGTGAAAGTACCAGAGATGATGTTGTTTCTGGAATTGATCAGTTCACAAGTGCTCGCAGCCGTTTGCAGGTTCTAATTGTTTCTTATGAAACATTCCGAATGCATTGTACAAAGTTTAGCCACAGTGAATCATGTGACCTTCTCATATGTGATGAGGCTCACAGGCTAAAGAATGACCAAACGATGACGAATAGGGCATTGGCTGCTCTTTCATGCAAGCGGAGGATTTTGTTGTCAGGAACTCCAATGCAGAATGACCTAGAAGAGTTCTTTGCCATGGTCAACTTCACTAATCCAGGAATCTTAGGTGATGCTGCACATTTTCGTCGCTACTATGAGGCACCTATTATTTGTGGAAGAGAACCTAATGCCTGTGAAGAAGAGAGGAAACTAGGTCTTGAGCGCTCTTCAGAACTCAGTGCAAAAGTTAATCAGTTTATACTGCGGAGGACTAATGCACTACTTTCAAATCACCTGCCACCAAAGATAATTGAAGTTGTCTGTTGCAAGTTGACTCCTCTCCAATTGGACCTTTATAACCATTTTATACATTCCAAAAATGTTAAACGAGCAATCTCAGAAGACACAAAGCAAACAAAAATATTAGCTTATATAACTGCTTTAAAGAAGCTATGCAATCATCCAAAGCTCATCTATGATACTATAAAAAGTGGAAGTCCAGGAACTTCAGGTTTTGAGGACTGCATTCGCTTTTTCCCTCCACAGATGTTCTCTGGAAGATCTGGATCATGGACTGGCGGAGATGGGGCTTGGGTTGAACTGTCAGGAAAGATGCATGTTTTAGCTAGGTTACTTGCCCAATTACGAAAGAAGACTGATGACCGTATTGTCCTTGTCTCAAACTATACGCAGACACTGGACCTTTTTGCCCAATTGTGTCGAGAAAGAAGATACCCATATTTGAGGCTTGACGGAACAACTTCTATCAGTAAAAGACAGAAATTAGTAAACCAATTCAATGACCCCTCAAAGGATGAATTTGCATTTCTCTTAAGCAGTAAGGCTGGTGGCTGTGGTCTCAATTTGATAGGCGGAAATCGACTGGTGTTGTTTGATCCTGATTGGAATCCAGCCAATGATAAACAAGCTGCTGCTAGAGTTTGGAGGGATGGGCAAAAGAAGAGAGTATACATTTATAGATTTTTGAGTACTGGAACAATTGAAGAAAAGGTCTACCAGCGACAGATGTCAAAAGAAGGGCTTCAAAAAGTTATTCAGAAGGAACAAGCCGATAGCCCGATGGCACAGGGGAATCTTTCAATTGAGGACCTACGCGATTTGTTCACATTTCATGAGAATGTGAGGTCTGATGTGCATGAGAAGATGAACTGCAACCGTTGTCAAACTTATCATGATACACCTCAGAGTACAGCAGAGGAAGATATAAACCAATCAACTTATAAAAGCAGTGAGTCGGCACCAGAAATCTCTGATATAGGTGGATTTGCAGAAATTACTGGATGCTTACAGAAGTTAAAGAGCTCAGAAAAGCAGATCGGGACACCTCTAGAGGAAGATTTAAGTAACTGGGGGCATCATTTCTTCTCCACTTCAGTACCCGATGCTATTCTCCAAGCTTCAGCTGGTGATGAGGTCACTTTTGTTTTTACAAACCAAGTGGACGGAAAGCTTGTGCCTATAGAATCAAAAGCAAGTCCAAAGATGCAGGGAGAAGAAGGAAAAGAGAATATTTTCAAATCAAAGCAAAACCTGAATCAGAAATCCATGCCAATGTCTTGGCAAAGAAAACCACTTGAATCTCTTTCTTCGAGTGTAAATACTGCCAGAAGCACATTATCAGCCCCTTTCAGGCCCTCGCAAAACACCACTGTGCAATCAGGGATAACCTCTTTAAAGGGTTCAGTAGCATTGAAGCCTAAACTCTCTCTTGGAAATCGGTTGGCCCAGAAAAGATCTTCACCTGATACTGTTGAACATGATGATGATTTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000280 GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0033170 GO:0033554 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045003 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051701 GO:0051704 GO:0051716 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070887 GO:0071214 GO:0071241 GO:0071248 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0104004 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

962

Amino Acids

107.67

Weight (kDa)

5.98

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 213 - 386 5.9e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 216 - 521 6e-71 SNF2-related domain
DEAD PF00270 238 - 393 1.6e-08 DEAD/DEAH box helicase
Helicase_C PF00271 568 - 682 1.5e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1449, 2334
AarI CACCTGC 1 cut(s) 1395
AasI GACNNNNNNGTC 2 cut(s) 757, 1760
Acc36I ACCTGC 2 cut(s) 970, 1395
AccB1I GGYRCC 2 cut(s) 1252, 2350
AccI GTMKAC 2 cut(s) 2061, 2106
AccII CGCG 2 cut(s) 177, 2208
AccIII TCCGGA 1 cut(s) 224
AclWI GGATC 5 cut(s) 99, 186, 542, 1666, 1982
AcsI RAATTY 2 cut(s) 575, 1910
AcuI CTGAAG 5 cut(s) 591, 1305, 1584, 2469, 2496
AcyI GRCGYC 1 cut(s) 403
AfaI GTAC 6 cut(s) 174, 922, 1021, 2083, 2302, 2490
AfeI AGCGCT 1 cut(s) 1316
AfiI CCNNNNNNNGG 5 cut(s) 224, 437, 979, 1903, 2742
AflII CTTAAG 1 cut(s) 1924
AjnI CCWGG 4 cut(s) 431, 538, 1207, 1591
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AleI CACNNNNGTG 1 cut(s) 2762
Alw21I GWGCWC 4 cut(s) 141, 454, 967, 2417
Alw26I GTCTC 6 cut(s) 78, 123, 410, 882, 1774, 1955
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 5 cut(s) 99, 186, 542, 1666, 1982
AlwNI CAGNNNCTG 1 cut(s) 1789
Aor13HI TCCGGA 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 1316
AoxI GGCC 4 cut(s) 198, 822, 2744, 2835
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 6 cut(s) 701, 969, 1115, 1226, 2021, 2024
ApoI RAATTY 2 cut(s) 575, 1910
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 4 cut(s) 660, 1852, 2133, 2624
AspLEI GCGC 1 cut(s) 1317
AspS9I GGNCC 7 cut(s) 199, 616, 1441, 1792, 2200, 2745, 2836
AsuHPI GGTGA 5 cut(s) 328, 1232, 1376, 2531, 2844
AvaII GGWCC 4 cut(s) 616, 1441, 1792, 2200
BaeGI GKGCMC 1 cut(s) 454
BaeI ACNNNNGTAYC 2 cut(s) 2472, 2505
BanI GGYRCC 2 cut(s) 1252, 2350
BanII GRGCYC 1 cut(s) 2417
BarI GAAGNNNNNNTAC 2 cut(s) 2045, 2077
BauI CACGAG 2 cut(s) 81, 303
BbsI GAAGAC 3 cut(s) 126, 1500, 1751
Bbv12I GWGCWC 4 cut(s) 141, 454, 967, 2417
BbvI GCAGC 6 cut(s) 688, 981, 1102, 1213, 2008, 2011
BccI CCATC 6 cut(s) 374, 578, 794, 1670, 2036, 2167
BceAI ACGGC 2 cut(s) 205, 957
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 4 cut(s) 433, 540, 1209, 1593
BclI TGATCA 1 cut(s) 949
BcoDI GTCTC 6 cut(s) 78, 123, 410, 882, 1774, 1955
BfaI CTAG 6 cut(s) 483, 1172, 1304, 1719, 2028, 2441
BfmI CTRYAG 1 cut(s) 2571
BfoI RGCGCY 1 cut(s) 1318
BfrI CTTAAG 1 cut(s) 1924
BfuAI ACCTGC 2 cut(s) 970, 1395
BglII AGATCT 2 cut(s) 1652, 2846
BisI GCNGC 8 cut(s) 218, 268, 702, 970, 1116, 1227, 2022, 2025
BlsI GCNGC 8 cut(s) 219, 269, 703, 971, 1117, 1228, 2023, 2026
BmcAI AGTACT 1 cut(s) 2083
Bme1390I CCNGG 4 cut(s) 433, 540, 1209, 1593
Bme18I GGWCC 4 cut(s) 616, 1441, 1792, 2200
BmgT120I GGNCC 7 cut(s) 199, 616, 1441, 1792, 2200, 2745, 2836
BmiI GGNNCC 3 cut(s) 618, 1254, 2352
BmrFI CCNGG 4 cut(s) 433, 540, 1209, 1593
BmrI ACTGGG 1 cut(s) 2470
BmsI GCATC 9 cut(s) 244, 487, 580, 1213, 1695, 2384, 2476, 2485, 2586
BmuI ACTGGG 1 cut(s) 2470
BoxI GACNNNNGTC 1 cut(s) 2119
BpiI GAAGAC 3 cut(s) 126, 1500, 1751
BplI GAGNNNNNCTC 2 cut(s) 885, 917
Bpu10I CCTNAGC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 1331
BsaBI GATNNNNATC 2 cut(s) 1657, 1998
BsaHI GRCGYC 1 cut(s) 403
BsaI GGTCTC 2 cut(s) 123, 1955
BsaJI CCNNGG 5 cut(s) 195, 431, 432, 790, 1188
BsaWI WCCGGW 2 cut(s) 224, 463
BsaXI ACNNNNNCTCC 6 cut(s) 120, 150, 318, 348, 879, 909
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 437, 979, 1903, 2742
Bse1I ACTGG 8 cut(s) 51, 843, 1672, 1794, 1980, 2089, 2395, 2465
Bse3DI GCAATG 2 cut(s) 752, 903
Bse8I GATNNNNATC 2 cut(s) 1657, 1998
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 4 cut(s) 433, 540, 1209, 1593
BseDI CCNNGG 5 cut(s) 195, 431, 432, 790, 1188
BseGI GGATG 7 cut(s) 589, 595, 722, 1552, 1912, 2047, 2399
BseJI GATNNNNATC 2 cut(s) 1657, 1998
BseLI CCNNNNNNNGG 5 cut(s) 224, 437, 979, 1903, 2742
BseMI GCAATG 2 cut(s) 752, 903
BseMII CTCAG 7 cut(s) 423, 492, 858, 1342, 1503, 2309, 2430
BseNI ACTGG 8 cut(s) 51, 843, 1672, 1794, 1980, 2089, 2395, 2465
BseRI GAGGAG 2 cut(s) 31, 1419
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 6 cut(s) 688, 981, 1102, 1213, 2008, 2011
BsgI GTGCAG 2 cut(s) 623, 1212
Bsh1236I CGCG 2 cut(s) 177, 2208
BshFI GGCC 4 cut(s) 200, 824, 2746, 2837
BshNI GGYRCC 2 cut(s) 1252, 2350
BsiHKAI GWGCWC 4 cut(s) 141, 454, 967, 2417
BsiSI CCGG 2 cut(s) 225, 464
BsiWI CGTACG 1 cut(s) 172
BslFI GGGAC 1 cut(s) 2446
BslI CCNNNNNNNGG 5 cut(s) 224, 437, 979, 1903, 2742
BsmAI GTCTC 6 cut(s) 78, 123, 410, 882, 1774, 1955
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 2446
BsmI GAATGC 2 cut(s) 1017, 1617
BsnI GGCC 4 cut(s) 200, 824, 2746, 2837
Bso31I GGTCTC 2 cut(s) 123, 1955
Bsp1286I GDGCHC 4 cut(s) 141, 454, 967, 2417
Bsp13I TCCGGA 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 1019
Bsp19I CCATGG 1 cut(s) 1188
BspANI GGCC 4 cut(s) 200, 824, 2746, 2837
BspCNI CTCAG 7 cut(s) 422, 493, 859, 1341, 1502, 2308, 2429
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 2 cut(s) 177, 2208
BspHI TCATGA 2 cut(s) 2224, 2284
BspLI GGNNCC 3 cut(s) 618, 1254, 2352
BspMI ACCTGC 2 cut(s) 970, 1395
BspPI GGATC 5 cut(s) 99, 186, 542, 1666, 1982
BspQI GCTCTTC 1 cut(s) 1324
BspT107I GGYRCC 2 cut(s) 1252, 2350
BspTI CTTAAG 1 cut(s) 1924
BspTNI GGTCTC 2 cut(s) 123, 1955
BsrDI GCAATG 2 cut(s) 752, 903
BsrGI TGTACA 1 cut(s) 1019
BsrI ACTGG 8 cut(s) 51, 843, 1672, 1794, 1980, 2089, 2395, 2465
BssECI CCNNGG 5 cut(s) 195, 431, 432, 790, 1188
BssNAI GTATAC 1 cut(s) 2062
BssNI GRCGYC 1 cut(s) 403
BssSI CACGAG 2 cut(s) 81, 303
BssT1I CCWWGG 3 cut(s) 195, 790, 1188
Bst1107I GTATAC 1 cut(s) 2062
Bst2BI CACGAG 2 cut(s) 81, 303
Bst2UI CCWGG 4 cut(s) 433, 540, 1209, 1593
Bst4CI ACNGT 9 cut(s) 334, 423, 456, 1037, 1695, 1757, 2270, 2764, 2863
Bst6I CTCTTC 7 cut(s) 300, 513, 1170, 1264, 1287, 1324, 2048
BstACI GRCGYC 1 cut(s) 403
BstAFI CTTAAG 1 cut(s) 1924
BstAPI GCANNNNNTGC 1 cut(s) 1115
BstAUI TGTACA 1 cut(s) 1019
BstC8I GCNNGC 6 cut(s) 202, 396, 415, 688, 967, 1130
BstDEI CTNAG 9 cut(s) 263, 409, 501, 867, 1216, 1328, 1489, 2295, 2416
BstDSI CCRYGG 1 cut(s) 1188
BstF5I GGATG 7 cut(s) 589, 595, 722, 1552, 1912, 2047, 2399
BstFNI CGCG 2 cut(s) 177, 2208
BstH2I RGCGCY 1 cut(s) 1318
BstHHI GCGC 1 cut(s) 1317
BstMAI GTCTC 6 cut(s) 78, 123, 410, 882, 1774, 1955
BstMWI GCNNNNNNNGC 4 cut(s) 217, 369, 400, 1115
BstNI CCWGG 4 cut(s) 433, 540, 1209, 1593
BstNSI RCATGY 1 cut(s) 1712
BstPAI GACNNNNGTC 1 cut(s) 2119
BstSCI CCNGG 4 cut(s) 431, 538, 1207, 1591
BstSFI CTRYAG 1 cut(s) 2571
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 2 cut(s) 177, 2208
BstV1I GCAGC 6 cut(s) 688, 981, 1102, 1213, 2008, 2011
BstV2I GAAGAC 3 cut(s) 126, 1500, 1751
BstX2I RGATCY 4 cut(s) 91, 191, 1652, 2846
BstXI CCANNNNNNTGG 1 cut(s) 2670
BstYI RGATCY 4 cut(s) 91, 191, 1652, 2846
BstZ17I GTATAC 1 cut(s) 2062
BsuRI GGCC 4 cut(s) 200, 824, 2746, 2837
BtgI CCRYGG 1 cut(s) 1188
BtsCI GGATG 7 cut(s) 589, 595, 722, 1552, 1912, 2047, 2399
BtsI GCAGTG 2 cut(s) 69, 2347
BtsIMutI CAGTG 6 cut(s) 69, 1042, 1336, 1787, 2347, 2760
BveI ACCTGC 2 cut(s) 970, 1395
Cac8I GCNNGC 6 cut(s) 202, 396, 415, 688, 967, 1130
CaiI CAGNNNCTG 1 cut(s) 1789
CciI TCATGA 2 cut(s) 2224, 2284
CfoI GCGC 1 cut(s) 1317
Cfr13I GGNCC 7 cut(s) 199, 616, 1441, 1792, 2200, 2745, 2836
CseI GACGC 1 cut(s) 392
Csp6I GTAC 6 cut(s) 173, 921, 1020, 2082, 2301, 2489
CspCI CAANNNNNGTGG 6 cut(s) 69, 104, 829, 864, 2532, 2567
CviQI GTAC 6 cut(s) 173, 921, 1020, 2082, 2301, 2489
DdeI CTNAG 9 cut(s) 263, 409, 501, 867, 1216, 1328, 1489, 2295, 2416
DraI TTTAAA 3 cut(s) 352, 1536, 2787
DrdI GACNNNNNNGTC 2 cut(s) 757, 1760
DseDI GACNNNNNNGTC 2 cut(s) 757, 1760
Eam1104I CTCTTC 7 cut(s) 300, 513, 1170, 1264, 1287, 1324, 2048
EarI CTCTTC 7 cut(s) 300, 513, 1170, 1264, 1287, 1324, 2048
EciI GGCGGA 3 cut(s) 204, 1686, 1980
Ecl136II GAGCTC 1 cut(s) 2415
Eco130I CCWWGG 3 cut(s) 195, 790, 1188
Eco24I GRGCYC 1 cut(s) 2417
Eco31I GGTCTC 2 cut(s) 123, 1955
Eco47I GGWCC 4 cut(s) 616, 1441, 1792, 2200
Eco47III AGCGCT 1 cut(s) 1316
Eco53kI GAGCTC 1 cut(s) 2415
Eco57I CTGAAG 5 cut(s) 591, 1305, 1584, 2469, 2496
EcoICRI GAGCTC 1 cut(s) 2415
EcoO109I RGGNCCY 2 cut(s) 2200, 2745
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 4 cut(s) 431, 538, 1207, 1591
EcoT14I CCWWGG 3 cut(s) 195, 790, 1188
EcoT22I ATGCAT 2 cut(s) 1017, 1710
EcoT38I GRGCYC 1 cut(s) 2417
ErhI CCWWGG 3 cut(s) 195, 790, 1188
Esp3I CGTCTC 1 cut(s) 410
FalI AAGNNNNNCTT 2 cut(s) 2118, 2150
FaqI GGGAC 1 cut(s) 2446
FauI CCCGC 2 cut(s) 209, 679
FauNDI CATATG 1 cut(s) 1058
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 2 cut(s) 2061, 2106
Fnu4HI GCNGC 8 cut(s) 218, 268, 702, 970, 1116, 1227, 2022, 2025
FokI GGATG 7 cut(s) 596, 602, 729, 1539, 1919, 2054, 2406
FriOI GRGCYC 1 cut(s) 2417
Fsp4HI GCNGC 8 cut(s) 218, 268, 702, 970, 1116, 1227, 2022, 2025
FspBI CTAG 6 cut(s) 483, 1172, 1304, 1719, 2028, 2441
GlaI GCGC 1 cut(s) 1316
GluI GCNGC 8 cut(s) 218, 268, 702, 970, 1116, 1227, 2022, 2025
HaeII RGCGCY 1 cut(s) 1318
HaeIII GGCC 4 cut(s) 200, 824, 2746, 2837
HapII CCGG 2 cut(s) 225, 464
HgaI GACGC 1 cut(s) 392
HhaI GCGC 1 cut(s) 1317
Hin1I GRCGYC 1 cut(s) 403
Hin6I GCGC 1 cut(s) 1315
HinP1I GCGC 1 cut(s) 1315
HincII GTYRAC 2 cut(s) 1195, 1425
HindII GTYRAC 2 cut(s) 1195, 1425
HindIII AAGCTT 2 cut(s) 2508, 2561
HpaII CCGG 2 cut(s) 225, 464
HphI GGTGA 5 cut(s) 328, 1232, 1376, 2531, 2844
Hpy99I CGWCG 5 cut(s) 65, 170, 173, 189, 1242
HpyCH4III ACNGT 9 cut(s) 334, 423, 456, 1037, 1695, 1757, 2270, 2764, 2863
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 369, 400, 1115
HpyF3I CTNAG 9 cut(s) 263, 409, 501, 867, 1216, 1328, 1489, 2295, 2416
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 403
HspAI GCGC 1 cut(s) 1315
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 949
LguI GCTCTTC 1 cut(s) 1324
LmnI GCTCC 2 cut(s) 326, 368
Lsp1109I GCAGC 6 cut(s) 688, 981, 1102, 1213, 2008, 2011
LweI GCATC 9 cut(s) 244, 487, 580, 1213, 1695, 2384, 2476, 2485, 2586
MaeI CTAG 6 cut(s) 483, 1172, 1304, 1719, 2028, 2441
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 7 cut(s) 316, 526, 832, 1046, 1722, 2456, 2527
MfeI CAATTG 5 cut(s) 856, 1436, 1805, 2091, 2193
MflI RGATCY 4 cut(s) 91, 191, 1652, 2846
MhlI GDGCHC 4 cut(s) 141, 454, 967, 2417
MlyI GAGTC 2 cut(s) 1420, 2354
MmeI TCCRAC 2 cut(s) 167, 865
Mph1103I ATGCAT 2 cut(s) 1017, 1710
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 4 cut(s) 660, 1852, 2133, 2624
MslI CAYNNNNRTG 4 cut(s) 2229, 2663, 2762, 2874
MspA1I CMGCKG 1 cut(s) 2516
MspCI CTTAAG 1 cut(s) 1924
MspI CCGG 2 cut(s) 225, 464
MspR9I CCNGG 4 cut(s) 433, 540, 1209, 1593
MunI CAATTG 5 cut(s) 856, 1436, 1805, 2091, 2193
Mva1269I GAATGC 2 cut(s) 1017, 1617
MvaI CCWGG 4 cut(s) 433, 540, 1209, 1593
MvnI CGCG 2 cut(s) 177, 2208
MwoI GCNNNNNNNGC 4 cut(s) 217, 369, 400, 1115
NcoI CCATGG 1 cut(s) 1188
NdeI CATATG 1 cut(s) 1058
NlaIV GGNNCC 3 cut(s) 618, 1254, 2352
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 5 cut(s) 316, 526, 832, 1046, 2527
NsiI ATGCAT 2 cut(s) 1017, 1710
NspI RCATGY 1 cut(s) 1712
OliI CACNNNNGTG 1 cut(s) 2762
PagI TCATGA 2 cut(s) 2224, 2284
PaqCI CACCTGC 1 cut(s) 1395
PasI CCCWGGG 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 1324
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 181, 637
PctI GAATGC 2 cut(s) 1017, 1617
PdmI GAANNNNTTC 4 cut(s) 660, 1852, 2133, 2624
Pfl23II CGTACG 1 cut(s) 172
PfoI TCCNGGA 2 cut(s) 1207, 1591
PkrI GCNGC 8 cut(s) 219, 269, 703, 971, 1117, 1228, 2023, 2026
PleI GAGTC 2 cut(s) 1420, 2353
PpsI GAGTC 2 cut(s) 1420, 2353
PpuMI RGGWCCY 1 cut(s) 2200
PshAI GACNNNNGTC 1 cut(s) 2119
PsiI TTATAA 2 cut(s) 1449, 2334
Psp124BI GAGCTC 1 cut(s) 2417
Psp5II RGGWCCY 1 cut(s) 2200
Psp6I CCWGG 4 cut(s) 431, 538, 1207, 1591
PspGI CCWGG 4 cut(s) 431, 538, 1207, 1591
PspLI CGTACG 1 cut(s) 172
PspN4I GGNNCC 3 cut(s) 618, 1254, 2352
PspPI GGNCC 7 cut(s) 199, 616, 1441, 1792, 2200, 2745, 2836
PspPPI RGGWCCY 1 cut(s) 2200
PstNI CAGNNNCTG 1 cut(s) 1789
PsuI RGATCY 4 cut(s) 91, 191, 1652, 2846
PvuII CAGCTG 1 cut(s) 2516
RsaI GTAC 6 cut(s) 174, 922, 1021, 2083, 2302, 2490
RsaNI GTAC 6 cut(s) 173, 921, 1020, 2082, 2301, 2489
RseI CAYNNNNRTG 4 cut(s) 2229, 2663, 2762, 2874
SacI GAGCTC 1 cut(s) 2417
SapI GCTCTTC 1 cut(s) 1324
SatI GCNGC 8 cut(s) 218, 268, 702, 970, 1116, 1227, 2022, 2025
Sau96I GGNCC 7 cut(s) 199, 616, 1441, 1792, 2200, 2745, 2836
ScaI AGTACT 1 cut(s) 2083
SchI GAGTC 2 cut(s) 1420, 2354
ScrFI CCNGG 4 cut(s) 433, 540, 1209, 1593
SduI GDGCHC 4 cut(s) 141, 454, 967, 2417
SfaNI GCATC 9 cut(s) 244, 487, 580, 1213, 1695, 2384, 2476, 2485, 2586
SfcI CTRYAG 1 cut(s) 2571
SinI GGWCC 4 cut(s) 616, 1441, 1792, 2200
SmiMI CAYNNNNRTG 4 cut(s) 2229, 2663, 2762, 2874
SmlI CTYRAG 2 cut(s) 1310, 1924
SmoI CTYRAG 2 cut(s) 1310, 1924
SspI AATATT 2 cut(s) 1515, 2623
SspMI CTAG 6 cut(s) 483, 1172, 1304, 1719, 2028, 2441
SstI GAGCTC 1 cut(s) 2417
StyD4I CCNGG 4 cut(s) 431, 538, 1207, 1591
StyI CCWWGG 3 cut(s) 195, 790, 1188
TaaI ACNGT 9 cut(s) 334, 423, 456, 1037, 1695, 1757, 2270, 2764, 2863
TaiI ACGT 1 cut(s) 174
TaqI TCGA 8 cut(s) 141, 242, 252, 579, 640, 1813, 1972, 2702
TatI WGTACW 3 cut(s) 1019, 2081, 2300
TauI GCSGC 2 cut(s) 220, 270
TscAI CASTG 6 cut(s) 76, 1042, 1336, 1794, 2347, 2767
TseFI GTSAC 5 cut(s) 316, 526, 832, 1046, 2527
TseI GCWGC 6 cut(s) 701, 969, 1115, 1226, 2021, 2024
Tsp45I GTSAC 5 cut(s) 316, 526, 832, 1046, 2527
TspDTI ATGAA 7 cut(s) 432, 658, 1014, 1113, 1923, 2213, 2273
TspGWI ACGGA 3 cut(s) 335, 1860, 2571
TspRI CASTG 6 cut(s) 76, 1042, 1336, 1794, 2347, 2767
Vha464I CTTAAG 1 cut(s) 1924
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 4 cut(s) 616, 1441, 1792, 2200
XapI RAATTY 2 cut(s) 575, 1910
XbaI TCTAGA 1 cut(s) 2440
XceI RCATGY 1 cut(s) 1712
XcmI CCANNNNNNNNNTGG 2 cut(s) 797, 2513
XmiI GTMKAC 2 cut(s) 2061, 2106
XmnI GAANNNNTTC 4 cut(s) 660, 1852, 2133, 2624
XspI CTAG 6 cut(s) 483, 1172, 1304, 1719, 2028, 2441
ZrmI AGTACT 1 cut(s) 2083
Zsp2I ATGCAT 2 cut(s) 1017, 1710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.