MD05G1082900.v1.1

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011249
Physical Location & Seq
Forward (+)
17304299 .. 17309420
5122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1082900.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGAGCACAGACTTATTTTTGTTGCAACTCTGTTATCTACTATTTCACTCCTCGATCTTTCTTCAGCTCGCGAGGAGGGACAGAGATGTATCAAGAACAGCGACTGCGACTCGGGATTGCACTGCGAGACGTGCGTTGCAGACGGCAACGTGGTGCCCAAATGCACCAGAATCAAACCCATCAGCCCCATTTCTCAGGTTCAAACGCTACCGTTTAACAAGTATTCATGGTTGACAACCCACAATTCGTTTGCAAGGTTAGGTGACAGGTCGGCGACTGGCTCATTAATTATCGCTCCTACTAACCAGCAGGACTCCATTACTGAGCAGCTTAACAATGGTGTTAGAGGGTTAATGCTGGATATGTATGACTTCCAAAATGGTATTTGGTTATGTCACTCGTTCGGCGGCAACTGCTACAATTACACATCTTTTCAACCCGCCATTAATGTGCTCAAAGAAATTCAAGCCTTTCTTCAAGCAAACCCATCAGAGATTGTGACCATCATCATTGAAGACTATGTTACATCACCAAAGGGCTTGACCAAAGTGTTTGATGCAGCTGGTTTGAGAAAATTCTGGTTCCCAGTGTCAAGAATGCCAAAAAAAGGCGGGGATTGGCCGACGGTTGCTGATATGATCCAGAAAAATCAGCGTTTGGTGGTTTTCACTTCAAAACGAGCAAAGGAGGCAAGTGAAGGGATAGCCTATGAGTGGAACTATGTGGTCGAAAACCAATATGGAAAGGGTGGAATGGTTGCTGGATCATGTCCAAATCGCGCCGAATCATCTTCCATGAACACAACATCAAAATCGTTGGTCTTGGTCAACTATTTTCGAGATGCTCCCGATATAACCCAAGCTTGCAAGGATAATTCAGATGCATTGCTTCGCATGGTGAATACGTGTTATGATGCGTCTTCTAAAAGATGGCCCAACTTTATTGCGGTTGATTTTTACAAGAGGAGTGATGGTGGGGGAGCTCCTAAAGCTACGGATGTCGCAAACGGTCATTTAGTTTGCGGGTGTGGAACCATTGCTAGCTGCAAGACCAATGTGAGCTTTGGAGTATGTCCACAGCCTGAGGCTGAAGTTGTCCCAAGAACTGCACCATCAACAACAAACTTTGGTTATTCGAATACGGGACCAGTTCAGCTGCTGTGGTTGTTTGGGACTCTTCTTGTGACACTTCTAATGTTGTTTTCGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

44.26

Weight (kDa)

7.87

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 59 - 350 1.2e-165 PI-PLC-like catalytic domain
PI-PLC_X PF26146 100 - 324 4.1e-27 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49740 AT3G19310
fragaria_vesca FvH4_2g05830 FvH4_2g05830 FvH4_2g05830 FvH4_2g05830
malus_domestica MD05G1082900.v1.1
prunus_persica Prupe.8G127500_v2.0.a1
pyrus_communis pycom05g07800
rosa_chinensis RchiOBHm_Chr6g0257791
rosa_laevigata RLG00000014724
rosa_multiflora Rmu_co8372415.1_g000001
rosa_roxburghii Rroxscaffold_7G00208950
rosa_rugosa Rorug05G0568000
rosa_samantha Rh6AG085000 Rh6BG079400 Rh6CG074400 Rh6DG071300
rosa_wichuraiana Rw0G011950 Rw6G007630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 2 cut(s) 72, 780
AciI CCGC 5 cut(s) 408, 441, 612, 947, 1023
AclWI GGATC 2 cut(s) 634, 772
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 2 cut(s) 462, 575
AcuI CTGAAG 2 cut(s) 48, 1110
AfiI CCNNNNNNNGG 2 cut(s) 608, 714
AflIII ACRYGT 1 cut(s) 905
AgsI TTSAA 6 cut(s) 203, 437, 467, 479, 515, 675
AjiI CACGTC 1 cut(s) 132
AluBI AGCT 9 cut(s) 68, 331, 563, 863, 983, 992, 1044, 1062, 1156
AluI AGCT 9 cut(s) 68, 331, 563, 863, 983, 992, 1044, 1062, 1156
Alw21I GWGCWC 3 cut(s) 9, 456, 985
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 2 cut(s) 634, 772
AlwNI CAGNNNCTG 2 cut(s) 105, 1159
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 2 cut(s) 620, 932
ApeKI GCWGC 4 cut(s) 328, 560, 1044, 1156
ApoI RAATTY 2 cut(s) 462, 575
AseI ATTAAT 2 cut(s) 287, 447
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 2 cut(s) 933, 1145
AsuHPI GGTGA 3 cut(s) 275, 522, 910
AsuII TTCGAA 1 cut(s) 1136
AsuNHI GCTAGC 1 cut(s) 1040
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 1145
AxyI CCTNAGG 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 159
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 985
BbsI GAAGAC 2 cut(s) 522, 912
Bbv12I GWGCWC 3 cut(s) 9, 456, 985
BbvI GCAGC 4 cut(s) 340, 572, 1031, 1143
BccI CCATC 6 cut(s) 188, 496, 512, 924, 965, 1120
BceAI ACGGC 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 1041
BfmI CTRYAG 1 cut(s) 1208
BisI GCNGC 5 cut(s) 329, 409, 561, 1045, 1157
BlsI GCNGC 5 cut(s) 330, 410, 562, 1046, 1158
Bme18I GGWCC 1 cut(s) 1145
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 933, 1145
BmiI GGNNCC 4 cut(s) 156, 584, 1033, 1146
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 4 cut(s) 547, 832, 871, 904
BmtI GCTAGC 1 cut(s) 1044
BmuI ACTGGG 1 cut(s) 581
BpiI GAAGAC 2 cut(s) 522, 912
Bpu14I TTCGAA 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 906
BsaXI ACNNNNNCTCC 2 cut(s) 958, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 608, 714
Bse1I ACTGG 3 cut(s) 283, 587, 1148
Bse21I CCTNAGG 1 cut(s) 1083
Bse3DI GCAATG 2 cut(s) 884, 1035
BseGI GGATG 1 cut(s) 1003
BseLI CCNNNNNNNGG 2 cut(s) 608, 714
BseMI GCAATG 2 cut(s) 884, 1035
BseMII CTCAG 3 cut(s) 209, 315, 1074
BseNI ACTGG 3 cut(s) 283, 587, 1148
BseRI GAGGAG 3 cut(s) 41, 89, 979
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 4 cut(s) 340, 572, 1031, 1143
BsgI GTGCAG 1 cut(s) 1092
Bsh1236I CGCG 2 cut(s) 72, 780
BshFI GGCC 2 cut(s) 622, 934
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 3 cut(s) 9, 456, 985
BsiHKCI CYCGRG 1 cut(s) 112
BslFI GGGAC 4 cut(s) 93, 1082, 1158, 1186
BslI CCNNNNNNNGG 2 cut(s) 608, 714
BsmAI GTCTC 1 cut(s) 122
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 4 cut(s) 93, 1082, 1158, 1186
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 2 cut(s) 622, 934
BsoBI CYCGRG 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 1136
Bsp1286I GDGCHC 4 cut(s) 9, 159, 456, 985
Bsp143I GATC 3 cut(s) 55, 639, 764
Bsp68I TCGCGA 1 cut(s) 72
BspACI CCGC 5 cut(s) 408, 441, 612, 947, 1023
BspANI GGCC 2 cut(s) 622, 934
BspCNI CTCAG 3 cut(s) 208, 316, 1075
BspFNI CGCG 2 cut(s) 72, 780
BspLI GGNNCC 4 cut(s) 156, 584, 1033, 1146
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 2 cut(s) 634, 772
BspT104I TTCGAA 1 cut(s) 1136
BspT107I GGYRCC 1 cut(s) 154
BsrDI GCAATG 2 cut(s) 884, 1035
BsrI ACTGG 3 cut(s) 283, 587, 1148
BssMI GATC 3 cut(s) 55, 639, 764
Bst4CI ACNGT 3 cut(s) 213, 628, 1010
Bst6I CTCTTC 1 cut(s) 1182
BstBAI YACGTR 1 cut(s) 906
BstBI TTCGAA 1 cut(s) 1136
BstC8I GCNNGC 3 cut(s) 70, 865, 1042
BstDEI CTNAG 3 cut(s) 195, 324, 1083
BstF5I GGATG 1 cut(s) 1003
BstFNI CGCG 2 cut(s) 72, 780
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 3 cut(s) 58, 642, 767
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 3 cut(s) 55, 639, 764
BstMWI GCNNNNNNNGC 4 cut(s) 132, 414, 689, 989
BstSFI CTRYAG 1 cut(s) 1208
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 2 cut(s) 72, 780
BstV1I GCAGC 4 cut(s) 340, 572, 1031, 1143
BstV2I GAAGAC 2 cut(s) 522, 912
Bsu36I CCTNAGG 1 cut(s) 1083
BsuRI GGCC 2 cut(s) 622, 934
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 1003
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 121, 594
BtuMI TCGCGA 1 cut(s) 72
Cac8I GCNNGC 3 cut(s) 70, 865, 1042
CaiI CAGNNNCTG 2 cut(s) 105, 1159
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 933, 1145
CseI GACGC 1 cut(s) 906
CviAII CATG 4 cut(s) 228, 768, 796, 895
DdeI CTNAG 3 cut(s) 195, 324, 1083
DpnI GATC 3 cut(s) 57, 641, 766
DpnII GATC 3 cut(s) 55, 639, 764
EaeI YGGCCR 1 cut(s) 620
Eam1104I CTCTTC 1 cut(s) 1182
EarI CTCTTC 1 cut(s) 1182
Ecl136II GAGCTC 1 cut(s) 983
Eco24I GRGCYC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 1145
Eco53kI GAGCTC 1 cut(s) 983
Eco57I CTGAAG 2 cut(s) 48, 1110
Eco81I CCTNAGG 1 cut(s) 1083
Eco88I CYCGRG 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 983
EcoT22I ATGCAT 1 cut(s) 886
EcoT38I GRGCYC 1 cut(s) 985
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 4 cut(s) 231, 771, 799, 898
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 4 cut(s) 93, 1082, 1158, 1186
FatI CATG 4 cut(s) 227, 767, 795, 894
FauI CCCGC 3 cut(s) 448, 605, 1016
Fnu4HI GCNGC 5 cut(s) 329, 409, 561, 1045, 1157
FokI GGATG 1 cut(s) 1010
FriOI GRGCYC 1 cut(s) 985
Fsp4HI GCNGC 5 cut(s) 329, 409, 561, 1045, 1157
FspBI CTAG 1 cut(s) 1041
GlaI GCGC 1 cut(s) 781
GluI GCNGC 5 cut(s) 329, 409, 561, 1045, 1157
HaeIII GGCC 2 cut(s) 622, 934
HgaI GACGC 1 cut(s) 906
HhaI GCGC 1 cut(s) 782
Hin1II CATG 4 cut(s) 231, 771, 799, 898
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HincII GTYRAC 2 cut(s) 234, 829
HindII GTYRAC 2 cut(s) 234, 829
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 5 cut(s) 110, 171, 314, 785, 1174
HphI GGTGA 3 cut(s) 275, 522, 910
Hpy166II GTNNAC 3 cut(s) 234, 829, 1076
Hpy188I TCNGA 2 cut(s) 493, 880
Hpy188III TCNNGA 7 cut(s) 71, 94, 114, 594, 643, 839, 848
Hpy8I GTNNAC 3 cut(s) 234, 829, 1076
Hpy99I CGWCG 1 cut(s) 628
HpyAV CCTTC 1 cut(s) 692
HpyCH4III ACNGT 3 cut(s) 213, 628, 1010
HpyCH4IV ACGT 3 cut(s) 131, 150, 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 414, 689, 989
HpyF3I CTNAG 3 cut(s) 195, 324, 1083
HpySE526I ACGT 3 cut(s) 131, 150, 905
Hsp92II CATG 4 cut(s) 231, 771, 799, 898
HspAI GCGC 1 cut(s) 780
Kzo9I GATC 3 cut(s) 55, 639, 764
LmnI GCTCC 5 cut(s) 4, 301, 850, 980, 988
Lsp1109I GCAGC 4 cut(s) 340, 572, 1031, 1143
LweI GCATC 4 cut(s) 547, 832, 871, 904
MaeI CTAG 1 cut(s) 1041
MaeII ACGT 3 cut(s) 131, 150, 905
MaeIII GTNAC 5 cut(s) 263, 395, 499, 523, 1183
MalI GATC 3 cut(s) 57, 641, 766
MboI GATC 3 cut(s) 55, 639, 764
MboII GAAGA 6 cut(s) 54, 467, 527, 783, 912, 1169
MhlI GDGCHC 4 cut(s) 9, 159, 456, 985
MluCI AATT 6 cut(s) 244, 288, 421, 462, 575, 874
MlyI GAGTC 3 cut(s) 104, 308, 1168
MnlI CCTC 7 cut(s) 62, 67, 70, 341, 682, 957, 1078
Mph1103I ATGCAT 1 cut(s) 886
MseI TTAA 5 cut(s) 216, 287, 333, 353, 447
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 563, 1156
Mva1269I GAATGC 1 cut(s) 603
MvnI CGCG 2 cut(s) 72, 780
MwoI GCNNNNNNNGC 4 cut(s) 132, 414, 689, 989
NdeII GATC 3 cut(s) 55, 639, 764
NheI GCTAGC 1 cut(s) 1040
NlaIII CATG 4 cut(s) 231, 771, 799, 898
NlaIV GGNNCC 4 cut(s) 156, 584, 1033, 1146
NmuCI GTSAC 4 cut(s) 263, 395, 499, 1183
NruI TCGCGA 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 886
NspV TTCGAA 1 cut(s) 1136
PctI GAATGC 1 cut(s) 603
PfeI GAWTC 2 cut(s) 171, 785
PkrI GCNGC 5 cut(s) 330, 410, 562, 1046, 1158
PleI GAGTC 3 cut(s) 104, 308, 1168
PpsI GAGTC 3 cut(s) 104, 308, 1168
Ppu21I YACGTR 1 cut(s) 906
PshBI ATTAAT 2 cut(s) 287, 447
Psp124BI GAGCTC 1 cut(s) 985
PspN4I GGNNCC 4 cut(s) 156, 584, 1033, 1146
PspPI GGNCC 2 cut(s) 933, 1145
PstNI CAGNNNCTG 2 cut(s) 105, 1159
PvuII CAGCTG 2 cut(s) 563, 1156
RruI TCGCGA 1 cut(s) 72
RseI CAYNNNNRTG 1 cut(s) 449
SacI GAGCTC 1 cut(s) 985
SaqAI TTAA 5 cut(s) 216, 287, 333, 353, 447
SatI GCNGC 5 cut(s) 329, 409, 561, 1045, 1157
Sau3AI GATC 3 cut(s) 55, 639, 764
Sau96I GGNCC 2 cut(s) 933, 1145
SchI GAGTC 3 cut(s) 104, 308, 1168
SduI GDGCHC 4 cut(s) 9, 159, 456, 985
SfaNI GCATC 4 cut(s) 547, 832, 871, 904
SfcI CTRYAG 1 cut(s) 1208
SfuI TTCGAA 1 cut(s) 1136
SinI GGWCC 1 cut(s) 1145
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 6 cut(s) 244, 288, 421, 462, 575, 874
SsiI CCGC 5 cut(s) 408, 441, 612, 947, 1023
SspMI CTAG 1 cut(s) 1041
SstI GAGCTC 1 cut(s) 985
TaaI ACNGT 3 cut(s) 213, 628, 1010
TaiI ACGT 3 cut(s) 134, 153, 908
TaqI TCGA 4 cut(s) 54, 729, 838, 1136
TasI AATT 6 cut(s) 244, 288, 421, 462, 575, 874
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 2 cut(s) 171, 785
Tru1I TTAA 5 cut(s) 216, 287, 333, 353, 447
Tru9I TTAA 5 cut(s) 216, 287, 333, 353, 447
TscAI CASTG 2 cut(s) 128, 594
TseFI GTSAC 4 cut(s) 263, 395, 499, 1183
TseI GCWGC 4 cut(s) 328, 560, 1044, 1156
Tsp45I GTSAC 4 cut(s) 263, 395, 499, 1183
TspDTI ATGAA 2 cut(s) 216, 812
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 2 cut(s) 128, 594
VpaK11BI GGWCC 1 cut(s) 1145
VspI ATTAAT 2 cut(s) 287, 447
XapI RAATTY 2 cut(s) 462, 575
XspI CTAG 1 cut(s) 1041
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.