Rorug05G0568000

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011249
Physical Location & Seq
Forward (+)
76100109 .. 76102123
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0568000.1

Sequence Viewer

Length: 1572 bp
ATGTCCTCTGCAATGCTTAATGATGCCGCATTATGGTTCCTCATTATTCTTAATGTGATTGTTTCAACCAAAGGATATCCAGTAATGCTTTCCACGTTGGGGTTGCTACTCTTCACAATCTATATCATACGCCCACTTATGCTGTTGATTGTGAAGAAAACACCTGAAGGAGAAGAAGTAAAGGAAATCTATATAGTTCCGATACTTCTTGGGGTTCTAGTCATGGCCTTTATCACCGACGCCCTATGCCAAGTGTATCAATTGGCTCCTTTTCTTCTAGGTTTAATCATACCAGATGGACCACCACTTGGTGCAACATTAACAGCCAAGACCGAATTCTTGGTCTCAGAACTTCTTCTGCCAGTGTTCTTTTTTCTTATTGGCAACAGCTTTATAGATGAACAATTTTTCACTCAGCTGATCTTGTCTGCACTAGTGATCACAATGATCGTAACACCAATTGTACAATTCTCATACACTCCTCAAAGACAACTCCGAAGATCAACTAAAGATTCACAGGTCAGAAACATCCAATCACAACGAAATAACTCCGGAACTTTTCAGATTCTTTGTTGTGTTCACAATGAAGAAAGTGCCCGCAGCATCATCACCTTCTTAGAAGCTTCAAATCCAACAGAAGCAAGCCCCATATGCATGTACATTGTCCACGTAGTTGATTTAATTGGCAGTTCTGCACCACAATTGACACCTTACAAGAAGACAAAGAAATTTAGGCGGTTGGATTCACCTACACATCAAATGATGCGGGCCTTCGAGAATTACTCAGAAAACTCAAGAGGACCAGTGATAATTCATCCATATACTATGGTGGCTCCTTATAAATCCATGCACGAGACCATTTTTCGTCTAGCTCGGGAAAAGCTCGCTCCTCTGATTATCATCTCATTCCCTGATAACCACCACCAGTCTATTGTTGGCACTGCTCCCACTAGCCCCATACGCCAATTGAACCTTAATTTACAACAATTTTCTCAGTGTACAATTGGAATACTAGTTGACAAAGGCTTGCCTTGTCGGCCAAGTGTCTCCAACTTCTCTTACATTGTGGCCGTATTTTTCATTGGTGGACCAGATGACCGTGAGACATTAGCATATGCATCACGCATGGCAGACAACTCTGACGTGGGCATCATAGTGTTCAGGATTCTCGTGCGTATCAAAAAGATAGACAGTTCTGAGGAAGAGGAGAGAGAGTTTAAGCTAGACGAGTCATTGTTGGATGAGTTTAAGCTAAGAAACACCAGGAATGATAGGTTGAATTGGCGTGATATTGAGGTGGATGATGGTGTTGAGACTGTTGAGGCTATTAAAAATTTGCAGGGTGAGTACGACCTAGTGATGGTGGGGCGGCGACATTTGGATGTGTCGTTGAGGGATGAAGAAATGACAGACTTATTGCACAATCCAGAGCTGGGAGTGATTGGTGACATGCTCGCTTCTTCAGATTTTTGTGGTGGAATGGTGAATGTGTTGGTGATGCAAGAAAGCAGGAGCTTGAGTTGTGGAGCTTATTGTAACTCACTCCATGATATGTTACTAAAGTATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

523

Amino Acids

58.97

Weight (kDa)

5.5

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_C PF23259 355 - 502 2.2e-10 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49740 AT3G19310
fragaria_vesca FvH4_2g05830 FvH4_2g05830 FvH4_2g05830 FvH4_2g05830
malus_domestica MD05G1082900.v1.1
prunus_persica Prupe.8G127500_v2.0.a1
pyrus_communis pycom05g07800
rosa_chinensis RchiOBHm_Chr6g0257791
rosa_laevigata RLG00000014724
rosa_multiflora Rmu_co8372415.1_g000001
rosa_roxburghii Rroxscaffold_7G00208950
rosa_rugosa Rorug05G0568000
rosa_samantha Rh6AG085000 Rh6BG079400 Rh6CG074400 Rh6DG071300
rosa_wichuraiana Rw0G011950 Rw6G007630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AccB7I CCANNNNNTGG 1 cut(s) 308
AccIII TCCGGA 1 cut(s) 551
AciI CCGC 5 cut(s) 27, 598, 736, 766, 1369
AcoI YGGCCR 2 cut(s) 1037, 1068
AcsI RAATTY 3 cut(s) 335, 728, 1333
AcuI CTGAAG 2 cut(s) 186, 1446
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 311
AfaI GTAC 4 cut(s) 465, 659, 1000, 1349
AfiI CCNNNNNNNGG 5 cut(s) 33, 99, 308, 1360, 1433
AgsI TTSAA 4 cut(s) 66, 627, 970, 1279
AhlI ACTAGT 2 cut(s) 433, 1012
AjiI CACGTC 1 cut(s) 1144
AjnI CCWGG 1 cut(s) 1262
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
Alw26I GTCTC 5 cut(s) 349, 848, 1051, 1097, 1307
Ama87I CYCGRG 1 cut(s) 873
Aor13HI TCCGGA 1 cut(s) 551
AoxI GGCC 4 cut(s) 225, 768, 1037, 1068
ApeKI GCWGC 1 cut(s) 600
ApoI RAATTY 3 cut(s) 335, 728, 1333
ArsI GACNNNNNNTTYG 2 cut(s) 327, 359
Asp700I GAANNNNTTC 1 cut(s) 354
AspS9I GGNCC 4 cut(s) 299, 768, 800, 1088
AsuHPI GGTGA 7 cut(s) 226, 601, 738, 1355, 1457, 1495, 1507
AvaI CYCGRG 1 cut(s) 873
AvaII GGWCC 3 cut(s) 299, 800, 1088
BaeGI GKGCMC 1 cut(s) 598
BauI CACGAG 2 cut(s) 851, 1169
BbsI GAAGAC 1 cut(s) 725
BbvI GCAGC 1 cut(s) 612
BccI CCATC 3 cut(s) 290, 1298, 1354
BceAI ACGGC 1 cut(s) 1055
BciT130I CCWGG 1 cut(s) 1264
BclI TGATCA 1 cut(s) 438
BcoDI GTCTC 5 cut(s) 349, 848, 1051, 1097, 1307
BcuI ACTAGT 2 cut(s) 433, 1012
BfaI CTAG 8 cut(s) 218, 278, 434, 869, 951, 1013, 1223, 1355
BglI GCCNNNNNGGC 1 cut(s) 1036
BisI GCNGC 3 cut(s) 27, 601, 1370
BlsI GCNGC 3 cut(s) 28, 602, 1371
Bme1390I CCNGG 1 cut(s) 1264
Bme18I GGWCC 3 cut(s) 299, 800, 1088
BmeT110I CYCGRG 1 cut(s) 873
BmgBI CACGTC 1 cut(s) 1144
BmgT120I GGNCC 4 cut(s) 299, 768, 800, 1088
BmiI GGNNCC 3 cut(s) 38, 267, 834
BmrFI CCNGG 1 cut(s) 1264
BmsI GCATC 6 cut(s) 13, 612, 753, 1127, 1158, 1488
BpiI GAAGAC 1 cut(s) 725
BplI GAGNNNNNCTC 2 cut(s) 767, 799
BpuEI CTTGAG 2 cut(s) 778, 1537
BsaAI YACGTR 1 cut(s) 670
BsaBI GATNNNNATC 1 cut(s) 899
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 2 cut(s) 349, 848
BsaWI WCCGGW 1 cut(s) 551
BsaXI ACNNNNNCTCC 6 cut(s) 162, 192, 1199, 1229, 1504, 1534
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 99, 308, 1360, 1433
Bse1I ACTGG 4 cut(s) 80, 362, 803, 925
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 899
BseAI TCCGGA 1 cut(s) 551
BseBI CCWGG 1 cut(s) 1264
BseGI GGATG 6 cut(s) 528, 814, 1246, 1306, 1387, 1402
BseJI GATNNNNATC 1 cut(s) 899
BseLI CCNNNNNNNGG 5 cut(s) 33, 99, 308, 1360, 1433
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 5 cut(s) 360, 428, 798, 1007, 1188
BseNI ACTGG 4 cut(s) 80, 362, 803, 925
BseRI GAGGAG 3 cut(s) 471, 879, 1220
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 1 cut(s) 612
BseYI CCCAGC 1 cut(s) 1432
BsgI GTGCAG 2 cut(s) 414, 678
BshFI GGCC 4 cut(s) 227, 770, 1039, 1070
BsiHKCI CYCGRG 1 cut(s) 873
BsiSI CCGG 1 cut(s) 552
BslI CCNNNNNNNGG 5 cut(s) 33, 99, 308, 1360, 1433
BsmAI GTCTC 5 cut(s) 349, 848, 1051, 1097, 1307
BsnI GGCC 4 cut(s) 227, 770, 1039, 1070
Bso31I GGTCTC 2 cut(s) 349, 848
BsoBI CYCGRG 1 cut(s) 873
Bsp1286I GDGCHC 1 cut(s) 598
Bsp13I TCCGGA 1 cut(s) 551
Bsp1407I TGTACA 3 cut(s) 463, 657, 998
Bsp143I GATC 4 cut(s) 420, 438, 447, 500
BspACI CCGC 5 cut(s) 27, 598, 736, 766, 1369
BspANI GGCC 4 cut(s) 227, 770, 1039, 1070
BspCNI CTCAG 5 cut(s) 359, 427, 797, 1006, 1189
BspEI TCCGGA 1 cut(s) 551
BspLI GGNNCC 3 cut(s) 38, 267, 834
BspTNI GGTCTC 2 cut(s) 349, 848
BsrDI GCAATG 1 cut(s) 18
BsrGI TGTACA 3 cut(s) 463, 657, 998
BsrI ACTGG 4 cut(s) 80, 362, 803, 925
BssMI GATC 4 cut(s) 420, 438, 447, 500
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 2 cut(s) 851, 1169
Bst2BI CACGAG 2 cut(s) 851, 1169
Bst2UI CCWGG 1 cut(s) 1264
Bst4CI ACNGT 3 cut(s) 1100, 1193, 1318
Bst6I CTCTTC 2 cut(s) 116, 1197
BstACI GRCGYC 1 cut(s) 240
BstAUI TGTACA 3 cut(s) 463, 657, 998
BstBAI YACGTR 1 cut(s) 670
BstC8I GCNNGC 6 cut(s) 598, 643, 768, 885, 1028, 1455
BstDEI CTNAG 7 cut(s) 346, 414, 616, 784, 993, 1197, 1253
BstF5I GGATG 6 cut(s) 528, 814, 1246, 1306, 1387, 1402
BstKTI GATC 4 cut(s) 423, 441, 450, 503
BstMAI GTCTC 5 cut(s) 349, 848, 1051, 1097, 1307
BstMBI GATC 4 cut(s) 420, 438, 447, 500
BstMWI GCNNNNNNNGC 3 cut(s) 651, 960, 1036
BstNI CCWGG 1 cut(s) 1264
BstNSI RCATGY 2 cut(s) 658, 1453
BstSCI CCNGG 1 cut(s) 1262
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 1 cut(s) 612
BstV2I GAAGAC 1 cut(s) 725
BsuRI GGCC 4 cut(s) 227, 770, 1039, 1070
BtrI CACGTC 1 cut(s) 1144
BtsCI GGATG 6 cut(s) 528, 814, 1246, 1306, 1387, 1402
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 4 cut(s) 369, 810, 939, 1001
Cac8I GCNNGC 6 cut(s) 598, 643, 768, 885, 1028, 1455
Cfr13I GGNCC 4 cut(s) 299, 768, 800, 1088
CseI GACGC 1 cut(s) 248
Csp6I GTAC 4 cut(s) 464, 658, 999, 1348
CviAII CATG 6 cut(s) 223, 655, 847, 1126, 1450, 1547
CviQI GTAC 4 cut(s) 464, 658, 999, 1348
DdeI CTNAG 7 cut(s) 346, 414, 616, 784, 993, 1197, 1253
DpnI GATC 4 cut(s) 422, 440, 449, 502
DpnII GATC 4 cut(s) 420, 438, 447, 500
DraIII CACNNNGTG 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 1037, 1068
Eam1104I CTCTTC 2 cut(s) 116, 1197
EarI CTCTTC 2 cut(s) 116, 1197
Eco31I GGTCTC 2 cut(s) 349, 848
Eco32I GATATC 1 cut(s) 77
Eco47I GGWCC 3 cut(s) 299, 800, 1088
Eco57I CTGAAG 2 cut(s) 186, 1446
Eco88I CYCGRG 1 cut(s) 873
EcoRI GAATTC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 1262
EcoRV GATATC 1 cut(s) 77
EcoT22I ATGCAT 2 cut(s) 656, 1120
FaeI CATG 6 cut(s) 226, 658, 850, 1129, 1453, 1550
FatI CATG 6 cut(s) 222, 654, 846, 1125, 1449, 1546
FauI CCCGC 2 cut(s) 605, 759
FauNDI CATATG 2 cut(s) 650, 1114
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 27, 601, 1370
FokI GGATG 6 cut(s) 515, 801, 1253, 1313, 1394, 1409
Fsp4HI GCNGC 3 cut(s) 27, 601, 1370
FspBI CTAG 8 cut(s) 218, 278, 434, 869, 951, 1013, 1223, 1355
GluI GCNGC 3 cut(s) 27, 601, 1370
GsaI CCCAGC 1 cut(s) 1436
HaeIII GGCC 4 cut(s) 227, 770, 1039, 1070
HapII CCGG 1 cut(s) 552
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 6 cut(s) 226, 658, 850, 1129, 1453, 1550
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 5 cut(s) 512, 565, 743, 1165, 1229
HpaII CCGG 1 cut(s) 552
HphI GGTGA 7 cut(s) 226, 601, 738, 1355, 1457, 1495, 1507
Hpy166II GTNNAC 5 cut(s) 580, 667, 999, 1018, 1088
Hpy188III TCNNGA 6 cut(s) 552, 775, 795, 875, 1162, 1427
Hpy8I GTNNAC 5 cut(s) 580, 667, 999, 1018, 1088
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 161, 622, 781
HpyCH4III ACNGT 3 cut(s) 1100, 1193, 1318
HpyCH4IV ACGT 3 cut(s) 95, 669, 1143
HpyF10VI GCNNNNNNNGC 3 cut(s) 651, 960, 1036
HpyF3I CTNAG 7 cut(s) 346, 414, 616, 784, 993, 1197, 1253
HpySE526I ACGT 3 cut(s) 95, 669, 1143
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 6 cut(s) 226, 658, 850, 1129, 1453, 1550
Kpn2I TCCGGA 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 4 cut(s) 420, 438, 447, 500
LmnI GCTCC 6 cut(s) 271, 838, 892, 949, 1512, 1526
Lsp1109I GCAGC 1 cut(s) 612
LweI GCATC 6 cut(s) 13, 612, 753, 1127, 1158, 1488
MaeI CTAG 8 cut(s) 218, 278, 434, 869, 951, 1013, 1223, 1355
MaeII ACGT 3 cut(s) 95, 669, 1143
MaeIII GTNAC 4 cut(s) 451, 1445, 1535, 1554
MalI GATC 4 cut(s) 422, 440, 449, 502
MboI GATC 4 cut(s) 420, 438, 447, 500
MfeI CAATTG 5 cut(s) 260, 459, 701, 965, 1002
MhlI GDGCHC 1 cut(s) 598
MlyI GAGTC 1 cut(s) 1238
MmeI TCCRAC 4 cut(s) 656, 720, 1074, 1218
Mph1103I ATGCAT 2 cut(s) 656, 1120
MroI TCCGGA 1 cut(s) 551
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 9 cut(s) 18, 51, 284, 320, 680, 975, 1218, 1248, 1329
MslI CAYNNNNRTG 3 cut(s) 653, 1154, 1380
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 1 cut(s) 552
MspR9I CCNGG 1 cut(s) 1264
MunI CAATTG 5 cut(s) 260, 459, 701, 965, 1002
MvaI CCWGG 1 cut(s) 1264
MwoI GCNNNNNNNGC 3 cut(s) 651, 960, 1036
NdeI CATATG 2 cut(s) 650, 1114
NdeII GATC 4 cut(s) 420, 438, 447, 500
NlaIII CATG 6 cut(s) 226, 658, 850, 1129, 1453, 1550
NlaIV GGNNCC 3 cut(s) 38, 267, 834
NmuCI GTSAC 1 cut(s) 1445
NsiI ATGCAT 2 cut(s) 656, 1120
NspI RCATGY 2 cut(s) 658, 1453
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 4 cut(s) 512, 565, 743, 1165
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 3 cut(s) 28, 602, 1371
PleI GAGTC 1 cut(s) 1237
PpsI GAGTC 1 cut(s) 1237
Ppu21I YACGTR 1 cut(s) 670
PsiI TTATAA 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 1262
PspFI CCCAGC 1 cut(s) 1432
PspGI CCWGG 1 cut(s) 1262
PspN4I GGNNCC 3 cut(s) 38, 267, 834
PspPI GGNCC 4 cut(s) 299, 768, 800, 1088
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 4 cut(s) 465, 659, 1000, 1349
RsaNI GTAC 4 cut(s) 464, 658, 999, 1348
RseI CAYNNNNRTG 3 cut(s) 653, 1154, 1380
SaqAI TTAA 9 cut(s) 18, 51, 284, 320, 680, 975, 1218, 1248, 1329
SatI GCNGC 3 cut(s) 27, 601, 1370
Sau3AI GATC 4 cut(s) 420, 438, 447, 500
Sau96I GGNCC 4 cut(s) 299, 768, 800, 1088
SchI GAGTC 1 cut(s) 1238
ScrFI CCNGG 1 cut(s) 1264
SduI GDGCHC 1 cut(s) 598
SfaNI GCATC 6 cut(s) 13, 612, 753, 1127, 1158, 1488
SinI GGWCC 3 cut(s) 299, 800, 1088
SmiMI CAYNNNNRTG 3 cut(s) 653, 1154, 1380
SmlI CTYRAG 2 cut(s) 793, 1516
SmoI CTYRAG 2 cut(s) 793, 1516
SpeI ACTAGT 2 cut(s) 433, 1012
SsiI CCGC 5 cut(s) 27, 598, 736, 766, 1369
SspMI CTAG 8 cut(s) 218, 278, 434, 869, 951, 1013, 1223, 1355
StyD4I CCNGG 1 cut(s) 1262
TaaI ACNGT 3 cut(s) 1100, 1193, 1318
TaiI ACGT 3 cut(s) 98, 672, 1146
TaqI TCGA 1 cut(s) 774
TaqII GACCGA 1 cut(s) 347
TatI WGTACW 3 cut(s) 463, 657, 998
TauI GCSGC 2 cut(s) 29, 1372
TfiI GAWTC 4 cut(s) 512, 565, 743, 1165
Tru1I TTAA 9 cut(s) 18, 51, 284, 320, 680, 975, 1218, 1248, 1329
Tru9I TTAA 9 cut(s) 18, 51, 284, 320, 680, 975, 1218, 1248, 1329
TscAI CASTG 4 cut(s) 369, 810, 946, 1001
TseFI GTSAC 1 cut(s) 1445
TseI GCWGC 1 cut(s) 600
Tsp45I GTSAC 1 cut(s) 1445
TspDTI ATGAA 5 cut(s) 414, 600, 803, 1069, 1413
TspRI CASTG 4 cut(s) 369, 810, 946, 1001
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 3 cut(s) 299, 800, 1088
XapI RAATTY 3 cut(s) 335, 728, 1333
XceI RCATGY 2 cut(s) 658, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 8 cut(s) 218, 278, 434, 869, 951, 1013, 1223, 1355
Zsp2I ATGCAT 2 cut(s) 656, 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.