Rw6G007630

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011249
Physical Location & Seq
Reverse (-)
12617129 .. 12621216
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007630.1

Sequence Viewer

Length: 1203 bp
ATGGATCATAGACTCTTGCTCATTGCAACTCTGTTTTCTTTGCTTTGTCTCCTTCACCTTTCTTCAGCCCGCAAGGAGGGAGAGAAATGCATCAAGAACAGTGACTGCGACTCAGGATTGCACTGCGAGACTTGCGTTGCAAACAATAATTTTTTGCCGAAATGCACTAGAATAAAGCCCATAAGTCCAATTTCTCAAGTGAAAACGCTGCCGTTTAACAGGTATTCCTGGTTAACAACCCACAATTCCTTTGCGAAACTCGGTGACAAGTCGGCGACGGGATCTGTGATTTTGGCTCCGACTAATCAGCAGGACTCCATCACCGATCAGCTTAATAATGGTGTTAGAGGCTTAATGCTGGACATGTATGACTATTTAGGTGACATTTGGTTATGTCACTCGTACGGTGGCAACTGCTACAACTACACAGCTTTTCAACCTGCCATTAGAGAACTGAAAGAAATTACAGCATTTCTTGAAGCAAACCCGTCTGAGATTGTGACAATCATAATTGAAGACTACGTTACGTCACCAAAGGGCTTGACCAAAGTGTTTGATGCTGCTGGCTTGAGAAAATTCTGGTTCCCAGTTTCAAGAATGCCCAAAAAGGGTGGAGATTGGCCAACAGTTGCTGATATGATCCAGAAAAATCAGCGTTTGCTAGTCTTCACTTCTAAGCAAAGAAAAGAGGCTGATGAAGGCATTGCCTACCAGTGGAGTTACATGGTGGAAAACCAATATGGAAATGGTGGCATGAAAGCTGGATCATGTCCAAACCGTGCGGAATCATCAGCCATGAACACAACAACGAGATCATTAGTCCTAGTGAACTATTTCCGAGATGTCCCAACCCTCCCTCAATCTTGCAAAGATAATTCAGATTCATTGCTTGACATGGTTAATACCTGTTACAATGTGGCTGCTAAAAGATGGCCTAACTTCATTGCCGTTGATTTTTACAAGAGAAGTGATGGTGGCGGCGCTCCTAAAGCTGTAGATGTGGTAAATGGTCATTTAGTTTGCGGGTGTCCAAGCATAGCCAACTGCAGGGCGAATGTGAGCTACGGAACATGCCCGCCTGAGGCTGCTATAGTCCCGGAAAGTGCACCAGAAATTGGTGCTAGCAGCTTTGGTTATTCAAATAGAGGACCAGTGTTGTATGGGCCTGTTCTTGTGACCCTTACCTTCTTGTTGTCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

44.04

Weight (kDa)

8.09

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 59 - 350 4.3e-163 PI-PLC-like catalytic domain
PI-PLC_X PF26146 98 - 324 2.5e-25 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49740 AT3G19310
fragaria_vesca FvH4_2g05830 FvH4_2g05830 FvH4_2g05830 FvH4_2g05830
malus_domestica MD05G1082900.v1.1
prunus_persica Prupe.8G127500_v2.0.a1
pyrus_communis pycom05g07800
rosa_chinensis RchiOBHm_Chr6g0257791
rosa_laevigata RLG00000014724
rosa_multiflora Rmu_co8372415.1_g000001
rosa_roxburghii Rroxscaffold_7G00208950
rosa_rugosa Rorug05G0568000
rosa_samantha Rh6AG085000 Rh6BG079400 Rh6CG074400 Rh6DG071300
rosa_wichuraiana Rw0G011950 Rw6G007630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 1115
AciI CCGC 5 cut(s) 70, 782, 978, 1023, 1076
AclWI GGATC 4 cut(s) 12, 289, 634, 772
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 6 cut(s) 76, 608, 714, 1047, 1081, 1115
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 5 cut(s) 437, 479, 515, 594, 1140
AjnI CCWGG 1 cut(s) 227
AluBI AGCT 6 cut(s) 331, 431, 761, 992, 1062, 1128
AluI AGCT 6 cut(s) 331, 431, 761, 992, 1062, 1128
Alw21I GWGCWC 1 cut(s) 1108
Alw26I GTCTC 2 cut(s) 53, 122
Alw44I GTGCAC 1 cut(s) 1104
AlwI GGATC 4 cut(s) 12, 289, 634, 772
AlwNI CAGNNNCTG 2 cut(s) 105, 632
AoxI GGCC 3 cut(s) 620, 932, 1163
ApaLI GTGCAC 1 cut(s) 1104
ApeKI GCWGC 5 cut(s) 208, 560, 920, 1085, 1125
ApoI RAATTY 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 983
AspS9I GGNCC 2 cut(s) 1148, 1163
AsuC2I CCSGG 1 cut(s) 1097
AsuHPI GGTGA 5 cut(s) 47, 275, 313, 392, 522
AsuNHI GCTAGC 1 cut(s) 1121
AvaII GGWCC 1 cut(s) 1148
AxyI CCTNAGG 1 cut(s) 1080
BaeGI GKGCMC 1 cut(s) 1108
BalI TGGCCA 1 cut(s) 622
BbsI GAAGAC 2 cut(s) 522, 658
Bbv12I GWGCWC 1 cut(s) 1108
BbvI GCAGC 5 cut(s) 195, 547, 907, 1072, 1137
BccI CCATC 3 cut(s) 326, 924, 965
BceAI ACGGC 2 cut(s) 196, 932
BciT130I CCWGG 1 cut(s) 229
BcnI CCSGG 1 cut(s) 1097
BcoDI GTCTC 2 cut(s) 53, 122
BfaI CTAG 4 cut(s) 168, 662, 824, 1122
BfmI CTRYAG 3 cut(s) 993, 1045, 1089
BfoI RGCGCY 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 6 cut(s) 209, 561, 921, 979, 1086, 1126
BlsI GCNGC 6 cut(s) 210, 562, 922, 980, 1087, 1127
Bme1390I CCNGG 2 cut(s) 229, 1097
Bme18I GGWCC 1 cut(s) 1148
BmgT120I GGNCC 2 cut(s) 1148, 1163
BmiI GGNNCC 2 cut(s) 297, 584
BmrFI CCNGG 2 cut(s) 229, 1097
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 2 cut(s) 99, 547
BmtI GCTAGC 1 cut(s) 1125
BmuI ACTGGG 1 cut(s) 581
BpiI GAAGAC 2 cut(s) 522, 658
BpuEI CTTGAG 2 cut(s) 180, 589
BpuMI CCSGG 1 cut(s) 1097
Bsc4I CCNNNNNNNGG 6 cut(s) 76, 608, 714, 1047, 1081, 1115
Bse1I ACTGG 3 cut(s) 587, 712, 1151
Bse21I CCTNAGG 1 cut(s) 1080
Bse3DI GCAATG 4 cut(s) 21, 702, 884, 942
BseBI CCWGG 1 cut(s) 229
BseLI CCNNNNNNNGG 6 cut(s) 76, 608, 714, 1047, 1081, 1115
BseMI GCAATG 4 cut(s) 21, 702, 884, 942
BseMII CTCAG 3 cut(s) 126, 483, 1071
BseNI ACTGG 3 cut(s) 587, 712, 1151
BseSI GKGCMC 1 cut(s) 1108
BseXI GCAGC 5 cut(s) 195, 547, 907, 1072, 1137
BshFI GGCC 3 cut(s) 622, 934, 1165
BsiHKAI GWGCWC 1 cut(s) 1108
BsiSI CCGG 1 cut(s) 1097
BsiWI CGTACG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 830, 1079
BslI CCNNNNNNNGG 6 cut(s) 76, 608, 714, 1047, 1081, 1115
BsmAI GTCTC 2 cut(s) 53, 122
BsmFI GGGAC 2 cut(s) 830, 1079
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 3 cut(s) 622, 934, 1165
Bsp1286I GDGCHC 1 cut(s) 1108
Bsp143I GATC 6 cut(s) 4, 281, 325, 639, 764, 812
BspACI CCGC 5 cut(s) 70, 782, 978, 1023, 1076
BspANI GGCC 3 cut(s) 622, 934, 1165
BspCNI CTCAG 3 cut(s) 125, 484, 1072
BspLI GGNNCC 2 cut(s) 297, 584
BspMAI CTGCAG 1 cut(s) 1049
BspMI ACCTGC 1 cut(s) 448
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 4 cut(s) 12, 289, 634, 772
BsrDI GCAATG 4 cut(s) 21, 702, 884, 942
BsrI ACTGG 3 cut(s) 587, 712, 1151
BssMI GATC 6 cut(s) 4, 281, 325, 639, 764, 812
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 4 cut(s) 101, 407, 628, 779
BstC8I GCNNGC 4 cut(s) 70, 565, 1076, 1123
BstDEI CTNAG 4 cut(s) 112, 492, 675, 1080
BstH2I RGCGCY 1 cut(s) 984
BstHHI GCGC 1 cut(s) 983
BstKTI GATC 6 cut(s) 7, 284, 328, 642, 767, 815
BstMAI GTCTC 2 cut(s) 53, 122
BstMBI GATC 6 cut(s) 4, 281, 325, 639, 764, 812
BstMWI GCNNNNNNNGC 2 cut(s) 132, 989
BstNI CCWGG 1 cut(s) 229
BstNSI RCATGY 2 cut(s) 367, 1074
BstSCI CCNGG 2 cut(s) 227, 1095
BstSFI CTRYAG 3 cut(s) 993, 1045, 1089
BstSLI GKGCMC 1 cut(s) 1108
BstV1I GCAGC 5 cut(s) 195, 547, 907, 1072, 1137
BstV2I GAAGAC 2 cut(s) 522, 658
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
Bsu36I CCTNAGG 1 cut(s) 1080
BsuRI GGCC 3 cut(s) 622, 934, 1165
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 4 cut(s) 106, 121, 719, 1158
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 4 cut(s) 70, 565, 1076, 1123
CaiI CAGNNNCTG 2 cut(s) 105, 632
CfoI GCGC 1 cut(s) 983
Cfr13I GGNCC 2 cut(s) 1148, 1163
Csp6I GTAC 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 7 cut(s) 364, 724, 754, 768, 796, 895, 1071
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 4 cut(s) 112, 492, 675, 1080
DpnI GATC 6 cut(s) 6, 283, 327, 641, 766, 814
DpnII GATC 6 cut(s) 4, 281, 325, 639, 764, 812
EaeI YGGCCR 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 1148
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 1080
EcoRII CCWGG 1 cut(s) 227
EcoT22I ATGCAT 1 cut(s) 92
FaeI CATG 7 cut(s) 367, 727, 757, 771, 799, 898, 1074
FaqI GGGAC 2 cut(s) 830, 1079
FatI CATG 7 cut(s) 363, 723, 753, 767, 795, 894, 1070
FauI CCCGC 3 cut(s) 77, 1016, 1083
Fnu4HI GCNGC 6 cut(s) 209, 561, 921, 979, 1086, 1126
Fsp4HI GCNGC 6 cut(s) 209, 561, 921, 979, 1086, 1126
FspBI CTAG 4 cut(s) 168, 662, 824, 1122
GlaI GCGC 1 cut(s) 982
GluI GCNGC 6 cut(s) 209, 561, 921, 979, 1086, 1126
HaeII RGCGCY 1 cut(s) 984
HaeIII GGCC 3 cut(s) 622, 934, 1165
HapII CCGG 1 cut(s) 1097
HhaI GCGC 1 cut(s) 983
Hin1II CATG 7 cut(s) 367, 727, 757, 771, 799, 898, 1074
Hin6I GCGC 1 cut(s) 981
HinP1I GCGC 1 cut(s) 981
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 5 cut(s) 12, 110, 314, 785, 881
HpaI GTTAAC 1 cut(s) 234
HpaII CCGG 1 cut(s) 1097
HphI GGTGA 5 cut(s) 47, 275, 313, 392, 522
Hpy166II GTNNAC 3 cut(s) 234, 829, 1106
Hpy188I TCNGA 4 cut(s) 300, 493, 839, 880
Hpy188III TCNNGA 5 cut(s) 94, 114, 476, 594, 643
Hpy8I GTNNAC 3 cut(s) 234, 829, 1106
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 3 cut(s) 62, 692, 1195
HpyCH4III ACNGT 4 cut(s) 101, 407, 628, 779
HpyCH4IV ACGT 2 cut(s) 522, 527
HpyCH4V TGCA 8 cut(s) 26, 90, 121, 140, 165, 867, 1047, 1106
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 989
HpyF3I CTNAG 4 cut(s) 112, 492, 675, 1080
HpySE526I ACGT 2 cut(s) 522, 527
Hsp92II CATG 7 cut(s) 367, 727, 757, 771, 799, 898, 1074
HspAI GCGC 1 cut(s) 981
KspAI GTTAAC 1 cut(s) 234
Kzo9I GATC 6 cut(s) 4, 281, 325, 639, 764, 812
LmnI GCTCC 2 cut(s) 301, 988
Lsp1109I GCAGC 5 cut(s) 195, 547, 907, 1072, 1137
LweI GCATC 2 cut(s) 99, 547
MaeI CTAG 4 cut(s) 168, 662, 824, 1122
MaeII ACGT 2 cut(s) 522, 527
MalI GATC 6 cut(s) 6, 283, 327, 641, 766, 814
MboI GATC 6 cut(s) 4, 281, 325, 639, 764, 812
MboII GAAGA 3 cut(s) 54, 527, 658
MflI RGATCY 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 1108
MlsI TGGCCA 1 cut(s) 622
MluCI AATT 8 cut(s) 148, 189, 244, 462, 510, 575, 874, 1113
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 3 cut(s) 6, 104, 308
MmeI TCCRAC 1 cut(s) 323
MnlI CCTC 7 cut(s) 70, 341, 682, 863, 867, 1075, 1139
Mox20I TGGCCA 1 cut(s) 622
Mph1103I ATGCAT 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 833
MscI TGGCCA 1 cut(s) 622
MseI TTAA 5 cut(s) 216, 233, 333, 353, 900
Msp20I TGGCCA 1 cut(s) 622
MspI CCGG 1 cut(s) 1097
MspR9I CCNGG 2 cut(s) 229, 1097
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 132, 989
NciI CCSGG 1 cut(s) 1097
NdeII GATC 6 cut(s) 4, 281, 325, 639, 764, 812
NheI GCTAGC 1 cut(s) 1121
NlaIII CATG 7 cut(s) 367, 727, 757, 771, 799, 898, 1074
NlaIV GGNNCC 2 cut(s) 297, 584
NmuCI GTSAC 7 cut(s) 101, 263, 380, 395, 499, 528, 1174
NsiI ATGCAT 1 cut(s) 92
NspI RCATGY 2 cut(s) 367, 1074
PciI ACATGT 1 cut(s) 363
PctI GAATGC 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 785, 881
Pfl23II CGTACG 1 cut(s) 402
PflMI CCANNNNNTGG 1 cut(s) 1115
PfoI TCCNGGA 1 cut(s) 1095
PkrI GCNGC 6 cut(s) 210, 562, 922, 980, 1087, 1127
PleI GAGTC 3 cut(s) 6, 104, 308
PpsI GAGTC 3 cut(s) 6, 104, 308
PscI ACATGT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 227
PspLI CGTACG 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 297, 584
PspPI GGNCC 2 cut(s) 1148, 1163
PstI CTGCAG 1 cut(s) 1049
PstNI CAGNNNCTG 2 cut(s) 105, 632
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 5 cut(s) 216, 233, 333, 353, 900
SatI GCNGC 6 cut(s) 209, 561, 921, 979, 1086, 1126
Sau3AI GATC 6 cut(s) 4, 281, 325, 639, 764, 812
Sau96I GGNCC 2 cut(s) 1148, 1163
SchI GAGTC 3 cut(s) 6, 104, 308
ScrFI CCNGG 2 cut(s) 229, 1097
SduI GDGCHC 1 cut(s) 1108
SfaNI GCATC 2 cut(s) 99, 547
SfcI CTRYAG 3 cut(s) 993, 1045, 1089
SinI GGWCC 1 cut(s) 1148
SmlI CTYRAG 2 cut(s) 195, 568
SmoI CTYRAG 2 cut(s) 195, 568
Sse9I AATT 8 cut(s) 148, 189, 244, 462, 510, 575, 874, 1113
SsiI CCGC 5 cut(s) 70, 782, 978, 1023, 1076
SspMI CTAG 4 cut(s) 168, 662, 824, 1122
StyD4I CCNGG 2 cut(s) 227, 1095
TaaI ACNGT 4 cut(s) 101, 407, 628, 779
TaiI ACGT 2 cut(s) 525, 530
TasI AATT 8 cut(s) 148, 189, 244, 462, 510, 575, 874, 1113
TauI GCSGC 1 cut(s) 981
TfiI GAWTC 2 cut(s) 785, 881
Tru1I TTAA 5 cut(s) 216, 233, 333, 353, 900
Tru9I TTAA 5 cut(s) 216, 233, 333, 353, 900
TscAI CASTG 4 cut(s) 106, 128, 719, 1158
TseFI GTSAC 7 cut(s) 101, 263, 380, 395, 499, 528, 1174
TseI GCWGC 5 cut(s) 208, 560, 920, 1085, 1125
Tsp45I GTSAC 7 cut(s) 101, 263, 380, 395, 499, 528, 1174
TspDTI ATGAA 5 cut(s) 711, 770, 812, 873, 931
TspGWI ACGGA 1 cut(s) 1080
TspRI CASTG 4 cut(s) 106, 128, 719, 1158
Van91I CCANNNNNTGG 1 cut(s) 1115
VneI GTGCAC 1 cut(s) 1104
VpaK11BI GGWCC 1 cut(s) 1148
XapI RAATTY 1 cut(s) 575
XceI RCATGY 2 cut(s) 367, 1074
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 4 cut(s) 168, 662, 824, 1122
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.