MD05G1100900.v1.1

Glycosyl hydrolase family 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010160
Physical Location & Seq
Forward (+)
20910860 .. 20914037
3178 bp
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UTR
Exon/CDS
Intron
MD05G1100900.v1.1.491

Sequence Viewer

Length: 1734 bp
ATGTTCACAGAAGCTTGCACCTTATTGCTTTCATGCATCTTTCTGTATTTTGGGCATGGCACCCATGCATCATACTACAACTACTCCACAACAACAAAGTGCTTGGTAGAGCCACTAGATGCCCTATATGGAGGAGGGATTATAGTAAATCCAGAATTTAATTACAACATTGAAAGATGGGAGGCTTTTGGAAAAGGGAAAATAGAAGAACGGATATCGAAGAAAGGAAACAGGTTCATGGTGGCACACAGTAGAAAACATCCACTAGACAGTTTCTCGCAGAAGGTTTGCGTTGAGAAAGGGAAAATCTATAGCTTTTCTGCTTCTGTTCAAGTGAGTGAAGGAAGTGGGATAGTAGCTGTTGTTTTCAAGTTTCCCAACCGCGATGCTGTGCGTGGTGGTGATGTAATAGCTGAACATGGGTGTTGGACACTGCTAAAAGGCGGTTTCGTAGCCAATTTCACAAGCCCTGTTGAGATTCTTTTTGAGGTACGCAAAAATACATCAGTGGAAATAATGGTGGATAATGTCTCATTGCAACCTTTCACCAAGGAGGAATGGAGATCCCACCAAGACAAAAGCATTAAAGAGCTGCGAAAAAGTAAGGTGAGGTTCCATGTAACTCAGGCAAGTAAAAATCCTCTTAATGGTACTAAAGTCTCCATCAAGCAAGTTAAGTCGCATTTCCCATTTGGATGTGGCATGAACCATTACATCCTCACAAACTCCGATTACCAGAAATGGTTCGCCTCGAGATTTAAATGGACTACTTTCACCAATGAGATGAAATGGTACAGCACTGAGAAAGCACGTGGCCAAGAAAACTATACTATTGCAGATAACATGGTCAAATTTGCACAAAAAAATGGCATTTCTATTAGAGGTCACAATGTCTTCTGGGACGATCCCAAGTACCAGCCCGACTGGGTTAAATCCCTTTCCCCTGAGGAAGTACGAACAGCAGCAGCAAAGCGAATCAATTCAGTTGTTTCTAGATACCAAGGACAACTCATTGCTTGGGATGTAGTGAATGAGAATCTTCATTTTAGTTTCTATGAAGATAACCTCGGCGAGAATGCTTCAGCAGAATTCTATTCCACAGCTCAACAGCTTGATCCTGACACAACTATGTTCATGAATGAGTATAACACCATGGAATATAGTAGCGACGAGAAATCAAGTCCAGCCAACTACAAGAAAAAACTTGAGGAGATCTTATCATATCCCAGAAATGAAAATTTGTCGCTAGGAATAGGGTTGCAAGGTCACTTTGGTTCTGGTCAGCCAAATCTTGCGAACATGAGATCTACTTTAGACATGTTAGGTGCAATCGGACTGCCAATATGGCTTACAGAAGTGGATGTGGAAAAAGACCCGAATCAGGCTCAGTATTTGGAAGAGGTACTAAGGGAGGGATATTCTCATCCGGCGGTTCAAGGGATCATCATGTTTGTAGGTCCAGAGATAGCCGGGTTTAATGTGACCACCCTTGTAGATAAGAACTTCAAGAATACACCAGCAGGAGATGTTGTGGATAAGCTGATTGAGGAGTGGAACTCTAACACTCAGGAAATCATAGCAGATGATCAAGGATATATCGATGTTTCTTTGTTCCACGGTGATTATGAGATAACTGCAGAAGGCCGCGTTGCTAATACCTCGGCTACCCTAAGTCTTAGGGTTGCACGAGCTCATGAACCGCAGGCAATTGTTCACGTTCATATTGATACTTGA

Protein Analysis

578

Amino Acids

65.27

Weight (kDa)

5.98

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 225 - 482 1.8e-44 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14690 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33820
fragaria_vesca FvH4_2g03791
malus_domestica MD05G1100900.v1.1
prunus_persica Prupe.8G145600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252821
rosa_laevigata RLG00000015063
rosa_multiflora Rmu_co8501457.1_g000001
rosa_roxburghii Rroxscaffold_7G00212400
rosa_rugosa Rorug05G0538800 Rorug05G0538800
rosa_samantha Rh6AG054900 Rh6BG048900 Rh6CG047800 Rh6DG043300
rosa_wichuraiana Rw6G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccII CGCG 2 cut(s) 384, 1647
AciI CCGC 5 cut(s) 382, 444, 1430, 1645, 1700
AclWI GGATC 4 cut(s) 558, 899, 1109, 1448
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 4 cut(s) 155, 851, 1088, 1237
AcuI CTGAAG 1 cut(s) 1065
AcvI CACGTG 1 cut(s) 812
AfaI GTAC 6 cut(s) 492, 652, 794, 914, 954, 1404
AfiI CCNNNNNNNGG 2 cut(s) 647, 1381
AflIII ACRYGT 1 cut(s) 1317
AgsI TTSAA 5 cut(s) 173, 332, 370, 1436, 1507
AluBI AGCT 9 cut(s) 14, 315, 359, 413, 592, 1103, 1111, 1540, 1691
AluI AGCT 9 cut(s) 14, 315, 359, 413, 592, 1103, 1111, 1540, 1691
Alw21I GWGCWC 1 cut(s) 1693
Alw26I GTCTC 2 cut(s) 535, 664
AlwI GGATC 4 cut(s) 558, 899, 1109, 1448
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 2 cut(s) 814, 1642
ApeKI GCWGC 3 cut(s) 592, 962, 965
ApoI RAATTY 4 cut(s) 155, 851, 1088, 1237
Asp700I GAANNNNTTC 2 cut(s) 743, 979
AspS9I GGNCC 1 cut(s) 1457
AsuC2I CCSGG 1 cut(s) 1471
AsuHPI GGTGA 5 cut(s) 413, 538, 619, 766, 1631
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 1 cut(s) 1457
AxyI CCTNAGG 1 cut(s) 945
BaeI ACNNNNGTAYC 2 cut(s) 642, 675
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 1693
BauI CACGAG 1 cut(s) 1686
BbrPI CACGTG 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 886
Bbv12I GWGCWC 1 cut(s) 1693
BbvI GCAGC 3 cut(s) 579, 974, 977
BccI CCATC 2 cut(s) 171, 671
BclI TGATCA 1 cut(s) 1585
BcnI CCSGG 1 cut(s) 1471
BcoDI GTCTC 2 cut(s) 535, 664
BfaI CTAG 4 cut(s) 116, 266, 993, 1247
BfmI CTRYAG 2 cut(s) 310, 1635
BglI GCCNNNNNGGC 1 cut(s) 1345
BglII AGATCT 2 cut(s) 1212, 1304
BisI GCNGC 4 cut(s) 593, 963, 966, 1645
BlsI GCNGC 4 cut(s) 594, 964, 967, 1646
Bme1390I CCNGG 1 cut(s) 1471
Bme18I GGWCC 1 cut(s) 1457
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 1 cut(s) 1457
BmiI GGNNCC 2 cut(s) 61, 614
BmrFI CCNGG 1 cut(s) 1471
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 4 cut(s) 45, 77, 109, 376
BmuI ACTGGG 1 cut(s) 934
BpiI GAAGAC 1 cut(s) 886
BplI GAGNNNNNCTC 2 cut(s) 1541, 1573
BpuEI CTTGAG 1 cut(s) 1226
BpuMI CCSGG 1 cut(s) 1471
Bsa29I ATCGAT 1 cut(s) 1599
BsaAI YACGTR 1 cut(s) 812
BsaJI CCNNGG 6 cut(s) 549, 1000, 1066, 1152, 1615, 1659
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1515, 1545
Bsc4I CCNNNNNNNGG 2 cut(s) 647, 1381
Bse1I ACTGG 1 cut(s) 929
Bse21I CCTNAGG 1 cut(s) 945
Bse3DI GCAATG 2 cut(s) 533, 1011
BseCI ATCGAT 1 cut(s) 1599
BseDI CCNNGG 6 cut(s) 549, 1000, 1066, 1152, 1615, 1659
BseGI GGATG 6 cut(s) 259, 701, 714, 1027, 1366, 1423
BseLI CCNNNNNNNGG 2 cut(s) 647, 1381
BseMI GCAATG 2 cut(s) 533, 1011
BseMII CTCAG 5 cut(s) 638, 792, 936, 1400, 1580
BseNI ACTGG 1 cut(s) 929
BseRI GAGGAG 3 cut(s) 147, 1223, 1562
BseXI GCAGC 3 cut(s) 579, 974, 977
Bsh1236I CGCG 2 cut(s) 384, 1647
BshFI GGCC 2 cut(s) 816, 1644
BshNI GGYRCC 1 cut(s) 59
BshVI ATCGAT 1 cut(s) 1599
BsiHKAI GWGCWC 1 cut(s) 1693
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 2 cut(s) 1427, 1470
BslFI GGGAC 1 cut(s) 914
BslI CCNNNNNNNGG 2 cut(s) 647, 1381
BsmAI GTCTC 2 cut(s) 535, 664
BsmFI GGGAC 1 cut(s) 914
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 2 cut(s) 816, 1644
BsoBI CYCGRG 1 cut(s) 751
Bsp1286I GDGCHC 1 cut(s) 1693
Bsp143I GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 5 cut(s) 382, 444, 1430, 1645, 1700
BspANI GGCC 2 cut(s) 816, 1644
BspCNI CTCAG 5 cut(s) 637, 793, 937, 1399, 1579
BspDI ATCGAT 1 cut(s) 1599
BspFNI CGCG 2 cut(s) 384, 1647
BspHI TCATGA 2 cut(s) 1134, 1693
BspLI GGNNCC 2 cut(s) 61, 614
BspMAI CTGCAG 1 cut(s) 1639
BspPI GGATC 4 cut(s) 558, 899, 1109, 1448
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 533, 1011
BsrI ACTGG 1 cut(s) 929
BssECI CCNNGG 6 cut(s) 549, 1000, 1066, 1152, 1615, 1659
BssMI GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
BssSI CACGAG 1 cut(s) 1686
BssT1I CCWWGG 3 cut(s) 549, 1000, 1152
Bst2BI CACGAG 1 cut(s) 1686
Bst4CI ACNGT 3 cut(s) 251, 272, 1619
Bst6I CTCTTC 1 cut(s) 1392
BstBAI YACGTR 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 16, 1704
BstDEI CTNAG 8 cut(s) 624, 801, 945, 1386, 1406, 1566, 1670, 1676
BstDSI CCRYGG 2 cut(s) 1152, 1615
BstF5I GGATG 6 cut(s) 259, 701, 714, 1027, 1366, 1423
BstFNI CGCG 2 cut(s) 384, 1647
BstKTI GATC 7 cut(s) 566, 907, 1117, 1215, 1307, 1443, 1588
BstMAI GTCTC 2 cut(s) 535, 664
BstMBI GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
BstMWI GCNNNNNNNGC 1 cut(s) 1345
BstNSI RCATGY 1 cut(s) 1321
BstSCI CCNGG 1 cut(s) 1469
BstSFI CTRYAG 2 cut(s) 310, 1635
BstUI CGCG 2 cut(s) 384, 1647
BstV1I GCAGC 3 cut(s) 579, 974, 977
BstV2I GAAGAC 1 cut(s) 886
BstX2I RGATCY 3 cut(s) 563, 1212, 1304
BstYI RGATCY 3 cut(s) 563, 1212, 1304
Bsu15I ATCGAT 1 cut(s) 1599
Bsu36I CCTNAGG 1 cut(s) 945
BsuRI GGCC 2 cut(s) 816, 1644
BsuTUI ATCGAT 1 cut(s) 1599
BtgI CCRYGG 2 cut(s) 1152, 1615
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 6 cut(s) 259, 701, 714, 1027, 1366, 1423
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 3 cut(s) 431, 513, 798
Cac8I GCNNGC 2 cut(s) 16, 1704
CciI TCATGA 2 cut(s) 1134, 1693
Cfr13I GGNCC 1 cut(s) 1457
ClaI ATCGAT 1 cut(s) 1599
Csp6I GTAC 6 cut(s) 491, 651, 793, 913, 953, 1403
CviQI GTAC 6 cut(s) 491, 651, 793, 913, 953, 1403
DdeI CTNAG 8 cut(s) 624, 801, 945, 1386, 1406, 1566, 1670, 1676
DpnI GATC 7 cut(s) 565, 906, 1116, 1214, 1306, 1442, 1587
DpnII GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
DraI TTTAAA 1 cut(s) 760
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 1 cut(s) 1392
EarI CTCTTC 1 cut(s) 1392
Ecl136II GAGCTC 1 cut(s) 1691
Eco130I CCWWGG 3 cut(s) 549, 1000, 1152
Eco24I GRGCYC 1 cut(s) 1693
Eco32I GATATC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 1457
Eco53kI GAGCTC 1 cut(s) 1691
Eco57I CTGAAG 1 cut(s) 1065
Eco72I CACGTG 1 cut(s) 812
Eco81I CCTNAGG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 751
EcoICRI GAGCTC 1 cut(s) 1691
EcoRI GAATTC 1 cut(s) 1088
EcoRV GATATC 1 cut(s) 216
EcoT14I CCWWGG 3 cut(s) 549, 1000, 1152
EcoT22I ATGCAT 2 cut(s) 38, 70
EcoT38I GRGCYC 1 cut(s) 1693
ErhI CCWWGG 3 cut(s) 549, 1000, 1152
FaqI GGGAC 1 cut(s) 914
FbaI TGATCA 1 cut(s) 1585
Fnu4HI GCNGC 4 cut(s) 593, 963, 966, 1645
FokI GGATG 6 cut(s) 246, 701, 708, 1034, 1373, 1410
FriOI GRGCYC 1 cut(s) 1693
Fsp4HI GCNGC 4 cut(s) 593, 963, 966, 1645
FspBI CTAG 4 cut(s) 116, 266, 993, 1247
GluI GCNGC 4 cut(s) 593, 963, 966, 1645
HaeIII GGCC 2 cut(s) 816, 1644
HapII CCGG 2 cut(s) 1427, 1470
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 478, 975, 1036, 1378
HpaII CCGG 2 cut(s) 1427, 1470
HphI GGTGA 5 cut(s) 413, 538, 619, 766, 1631
Hpy166II GTNNAC 2 cut(s) 6, 1714
Hpy188I TCNGA 2 cut(s) 730, 1334
Hpy188III TCNNGA 9 cut(s) 152, 753, 993, 1118, 1135, 1460, 1507, 1568, 1694
Hpy8I GTNNAC 2 cut(s) 6, 1714
Hpy99I CGWCG 1 cut(s) 1172
HpyAV CCTTC 3 cut(s) 277, 335, 1634
HpyCH4III ACNGT 3 cut(s) 251, 272, 1619
HpyCH4IV ACGT 2 cut(s) 811, 1716
HpyF10VI GCNNNNNNNGC 1 cut(s) 1345
HpyF3I CTNAG 8 cut(s) 624, 801, 945, 1386, 1406, 1566, 1670, 1676
HpySE526I ACGT 2 cut(s) 811, 1716
Ksp22I TGATCA 1 cut(s) 1585
Kzo9I GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
Lsp1109I GCAGC 3 cut(s) 579, 974, 977
LweI GCATC 4 cut(s) 45, 77, 109, 376
MaeI CTAG 4 cut(s) 116, 266, 993, 1247
MaeII ACGT 2 cut(s) 811, 1716
MaeIII GTNAC 4 cut(s) 619, 884, 1265, 1480
MalI GATC 7 cut(s) 565, 906, 1116, 1214, 1306, 1442, 1587
MboI GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
MboII GAAGA 6 cut(s) 218, 232, 886, 1031, 1070, 1409
MfeI CAATTG 1 cut(s) 1707
MflI RGATCY 3 cut(s) 563, 1212, 1304
MhlI GDGCHC 1 cut(s) 1693
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 8 cut(s) 155, 160, 457, 851, 979, 1088, 1237, 1707
MluNI TGGCCA 1 cut(s) 816
MmeI TCCRAC 1 cut(s) 407
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 2 cut(s) 38, 70
MroXI GAANNNNTTC 2 cut(s) 743, 979
MscI TGGCCA 1 cut(s) 816
MseI TTAA 7 cut(s) 159, 585, 645, 675, 759, 930, 1476
MslI CAYNNNNRTG 2 cut(s) 694, 1127
Msp20I TGGCCA 1 cut(s) 816
MspI CCGG 2 cut(s) 1427, 1470
MspR9I CCNGG 1 cut(s) 1471
MunI CAATTG 1 cut(s) 1707
Mva1269I GAATGC 1 cut(s) 1081
MvnI CGCG 2 cut(s) 384, 1647
MwoI GCNNNNNNNGC 1 cut(s) 1345
NciI CCSGG 1 cut(s) 1471
NcoI CCATGG 1 cut(s) 1152
NdeII GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
NlaIV GGNNCC 2 cut(s) 61, 614
NmeAIII GCCGAG 2 cut(s) 1047, 1640
NmuCI GTSAC 3 cut(s) 884, 1265, 1480
NsiI ATGCAT 2 cut(s) 38, 70
NspI RCATGY 1 cut(s) 1321
PaeR7I CTCGAG 1 cut(s) 751
PagI TCATGA 2 cut(s) 1134, 1693
PciI ACATGT 1 cut(s) 1317
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 2 cut(s) 743, 979
PfeI GAWTC 4 cut(s) 478, 975, 1036, 1378
PkrI GCNGC 4 cut(s) 594, 964, 967, 1646
PmaCI CACGTG 1 cut(s) 812
PmlI CACGTG 1 cut(s) 812
Ppu21I YACGTR 1 cut(s) 812
PscI ACATGT 1 cut(s) 1317
Psp124BI GAGCTC 1 cut(s) 1693
PspCI CACGTG 1 cut(s) 812
PspN4I GGNNCC 2 cut(s) 61, 614
PspPI GGNCC 1 cut(s) 1457
PstI CTGCAG 1 cut(s) 1639
PsuI RGATCY 3 cut(s) 563, 1212, 1304
RsaI GTAC 6 cut(s) 492, 652, 794, 914, 954, 1404
RsaNI GTAC 6 cut(s) 491, 651, 793, 913, 953, 1403
RseI CAYNNNNRTG 2 cut(s) 694, 1127
SacI GAGCTC 1 cut(s) 1693
SaqAI TTAA 7 cut(s) 159, 585, 645, 675, 759, 930, 1476
SatI GCNGC 4 cut(s) 593, 963, 966, 1645
Sau3AI GATC 7 cut(s) 563, 904, 1114, 1212, 1304, 1440, 1585
Sau96I GGNCC 1 cut(s) 1457
ScrFI CCNGG 1 cut(s) 1471
SduI GDGCHC 1 cut(s) 1693
SfaNI GCATC 4 cut(s) 45, 77, 109, 376
SfcI CTRYAG 2 cut(s) 310, 1635
Sfr274I CTCGAG 1 cut(s) 751
SinI GGWCC 1 cut(s) 1457
SlaI CTCGAG 1 cut(s) 751
SmiI ATTTAAAT 1 cut(s) 760
SmiMI CAYNNNNRTG 2 cut(s) 694, 1127
SmlI CTYRAG 2 cut(s) 751, 1205
SmoI CTYRAG 2 cut(s) 751, 1205
Sse9I AATT 8 cut(s) 155, 160, 457, 851, 979, 1088, 1237, 1707
SsiI CCGC 5 cut(s) 382, 444, 1430, 1645, 1700
SspMI CTAG 4 cut(s) 116, 266, 993, 1247
SstI GAGCTC 1 cut(s) 1693
StyD4I CCNGG 1 cut(s) 1469
StyI CCWWGG 3 cut(s) 549, 1000, 1152
SwaI ATTTAAAT 1 cut(s) 760
TaaI ACNGT 3 cut(s) 251, 272, 1619
TaiI ACGT 2 cut(s) 814, 1719
TaqI TCGA 3 cut(s) 218, 752, 1599
TasI AATT 8 cut(s) 155, 160, 457, 851, 979, 1088, 1237, 1707
TauI GCSGC 1 cut(s) 1647
TfiI GAWTC 4 cut(s) 478, 975, 1036, 1378
Tru1I TTAA 7 cut(s) 159, 585, 645, 675, 759, 930, 1476
Tru9I TTAA 7 cut(s) 159, 585, 645, 675, 759, 930, 1476
TscAI CASTG 3 cut(s) 438, 513, 805
TseFI GTSAC 3 cut(s) 884, 1265, 1480
TseI GCWGC 3 cut(s) 592, 962, 965
Tsp45I GTSAC 3 cut(s) 884, 1265, 1480
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 3 cut(s) 438, 513, 805
VpaK11BI GGWCC 1 cut(s) 1457
XapI RAATTY 4 cut(s) 155, 851, 1088, 1237
XbaI TCTAGA 1 cut(s) 992
XceI RCATGY 1 cut(s) 1321
XhoI CTCGAG 1 cut(s) 751
XmnI GAANNNNTTC 2 cut(s) 743, 979
XspI CTAG 4 cut(s) 116, 266, 993, 1247
Zsp2I ATGCAT 2 cut(s) 38, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.