Rh6DG043300

Glycosyl hydrolase family 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010160
Physical Location & Seq
Reverse (-)
4654845 .. 4658271
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG043300.1

Sequence Viewer

Length: 1686 bp
ATGTTTTTTTGCAATACGTTCTTGCTAATTATGTTTTCTGTTACAGGGGATAGGACCTCTGCTTCTGCCTACAACTACACGACTACAACAAAGTGCTTGAAAGAGCCCCTAGAAGCTTTATATGGAGGAGGGATTATAGTGAACCCAGTTAATCACAATACTGAAGGATGGAAAGCATTTGGACAAGGAAAAATCAAAGAACGAATATCAAAAACTGCATCAAACAGTTTCTCCCAGAAGGTTCAAGTTGAGCAAGGAAAAATCTATACTATTTCTGCTTCTGTCCAAGTCAGTGAAGGAAGTGAGATTGTAGCTGTTGTTTTTAAGTTCCCCACCGGCGAAAACATACGTGGTGGTGATGTGGTAGCTGAGCATGGTTGCTGGACCCTGCTAAAAGGTGGCATAGTAGCAAATTTCACAAGTTCGGTTGAGATTCTTTTTGAGAGCAAAAATACATCAGTGGAAATATGGGTGGACAATGTCTCATTACAACCATTCACCAAGGAGGAGTGGAAATCACACCAAGACAAAAGCATTATTGAGGGACGAAAGAGTAAGGTGAGGTTGCAGGTAACTCAGGCAAATAAAGCTCCTCTTACAGGTGCTAAAGTGTCCATCAAGCAGACGAAGTCGGACTTCCCATTCGGATGTGGCATGAACCATAACATCCTCACAAGCACCGATTACCAGAATTGGTTTGCATCAAGATTTAGATATACCACTTTCACCAATGAGATGAAGTGGTACAGCACTGAGAAAATACAAGGCCAAGAAAACTATACTATTGCAGATGGCATGGTGAAATTTGCTAAAGAGAATGGCATTTCTATTAGAGGTCACAATGTCTTCTGGGACGATCCAAAAATGCAGCCTGGCTGGGTTAACAATACCCTTTCCTCCATGGAACTCTGTAGAGCATCAGCAGAGAGAATCAATTCAGTGGTTTCTAGATACAGAGGACAACTAATTGCTTGGGATGTAATGAATGAGAATCTTCACTTCAGATTCTTTGAGGATAAACTTGGCGAGAACGCTTCTGCAGCATTCTACTACACAGCTCAACAGCTTGATCCTGGCACAATGATGTTCATGAATGAGTATAACACCATGGAATATAGTGGCGACGAGGCATCAAGTCCAGCCAACTACAAGAAGAAACTTGAAGAGATCTTGTCATATCCTGGAAATGCTAATCTGACAGCAGGAATAGGTTTGCAAGGTCACTTTGGTTCTGGTCAGCCAAATCTTGCTTACATGAGATCTGTTTTAGACTTTTTAGGTGCAACTGGATATCCAATATGGCTTACAGAAGTGAGTGTGGGAATAGGCCCATATCAGGCCCAGTACTTGGAAGAGGTACTAAGGGAGGGTTATTCGCATCCTGCGGTCCAAGGGATCATCATGTTCGTAGGTCCAATCACAGCTGGTTTTAATGAAACAACTCTTGTGGATAAGAACTTCAACAACACTCCATCAGGAGATGTTGTGGATAAGCTGATTGCTGAGTGGAAAACGGGGCCTCTGGAAATGACAACTGATGATCAAGGATTTATAGATGTTTCCTTGTTTCATGGAGATTATGAGGTTACAGCTGAAGACAATGTCACCAATTCCTCAGCTACTCTAAGCTTTAAGGTTACAAAAGATGAACCACAGGCATTTGTTCAAGTTCAGATTGATACTTGA

Protein Analysis

561

Amino Acids

62.51

Weight (kDa)

5.27

Isoelectric Point (pI)

21.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 209 - 461 5.5e-37 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14690 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33820
fragaria_vesca FvH4_2g03791
malus_domestica MD05G1100900.v1.1
prunus_persica Prupe.8G145600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252821
rosa_laevigata RLG00000015063
rosa_multiflora Rmu_co8501457.1_g000001
rosa_roxburghii Rroxscaffold_7G00212400
rosa_rugosa Rorug05G0538800 Rorug05G0538800
rosa_samantha Rh6AG054900 Rh6BG048900 Rh6CG047800 Rh6DG043300
rosa_wichuraiana Rw6G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 1348
AciI CCGC 1 cut(s) 1385
AclWI GGATC 3 cut(s) 851, 1064, 1403
AcsI RAATTY 2 cut(s) 412, 803
AcuI CTGAAG 3 cut(s) 183, 985, 1614
AfaI GTAC 3 cut(s) 746, 1346, 1359
AfiI CCNNNNNNNGG 3 cut(s) 599, 1336, 1348
AgsI TTSAA 5 cut(s) 100, 245, 1163, 1462, 1667
AjnI CCWGG 3 cut(s) 871, 1072, 1180
Alw26I GTCTC 1 cut(s) 487
AlwI GGATC 3 cut(s) 851, 1064, 1403
AoxI GGCC 4 cut(s) 766, 1327, 1338, 1517
ApeKI GCWGC 2 cut(s) 868, 1040
ApoI RAATTY 2 cut(s) 412, 803
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
Asp700I GAANNNNTTC 1 cut(s) 934
AspS9I GGNCC 7 cut(s) 54, 384, 1328, 1339, 1387, 1412, 1517
AsuHPI GGTGA 6 cut(s) 368, 490, 571, 718, 811, 1597
AvaII GGWCC 4 cut(s) 54, 384, 1387, 1412
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 838, 1602
BbvCI CCTCAGC 1 cut(s) 1615
BbvI GCAGC 2 cut(s) 880, 1052
BccI CCATC 4 cut(s) 162, 623, 785, 1480
BciT130I CCWGG 3 cut(s) 873, 1074, 1182
BclI TGATCA 1 cut(s) 1540
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 2 cut(s) 110, 948
BfmI CTRYAG 2 cut(s) 910, 1038
BfuAI ACCTGC 1 cut(s) 559
BglII AGATCT 2 cut(s) 1167, 1259
BisI GCNGC 2 cut(s) 869, 1041
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 2 cut(s) 870, 1042
BmcAI AGTACT 1 cut(s) 1346
Bme1390I CCNGG 3 cut(s) 873, 1074, 1182
Bme18I GGWCC 4 cut(s) 54, 384, 1387, 1412
BmgT120I GGNCC 7 cut(s) 54, 384, 1328, 1339, 1387, 1412, 1517
BmiI GGNNCC 2 cut(s) 386, 1518
BmrFI CCNGG 3 cut(s) 873, 1074, 1182
BmrI ACTGGG 2 cut(s) 140, 1336
BmsI GCATC 5 cut(s) 227, 710, 926, 1139, 1387
BmuI ACTGGG 2 cut(s) 140, 1336
BpiI GAAGAC 2 cut(s) 838, 1602
Bpu10I CCTNAGC 1 cut(s) 1615
Bpu1102I GCTNAGC 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 350
BsaJI CCNNGG 4 cut(s) 501, 900, 1107, 1390
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 1470, 1500
Bsc4I CCNNNNNNNGG 3 cut(s) 599, 1336, 1348
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 3 cut(s) 146, 1291, 1342
BseBI CCWGG 3 cut(s) 873, 1074, 1182
BseDI CCNNGG 4 cut(s) 501, 900, 1107, 1390
BseGI GGATG 5 cut(s) 173, 653, 666, 982, 1378
BseLI CCNNNNNNNGG 3 cut(s) 599, 1336, 1348
BseMII CTCAG 5 cut(s) 360, 590, 744, 1494, 1629
BseNI ACTGG 3 cut(s) 146, 1291, 1342
BseRI GAGGAG 3 cut(s) 141, 521, 582
BseXI GCAGC 2 cut(s) 880, 1052
BseYI CCCAGC 1 cut(s) 876
BshFI GGCC 4 cut(s) 768, 1329, 1340, 1519
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 2 cut(s) 558, 866
BslI CCNNNNNNNGG 3 cut(s) 599, 1336, 1348
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 2 cut(s) 558, 866
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 4 cut(s) 768, 1329, 1340, 1519
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
Bsp1720I GCTNAGC 1 cut(s) 369
Bsp19I CCATGG 2 cut(s) 900, 1107
BspACI CCGC 1 cut(s) 1385
BspANI GGCC 4 cut(s) 768, 1329, 1340, 1519
BspCNI CTCAG 5 cut(s) 361, 589, 745, 1495, 1628
BspHI TCATGA 1 cut(s) 1089
BspLI GGNNCC 2 cut(s) 386, 1518
BspMAI CTGCAG 1 cut(s) 1042
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 3 cut(s) 851, 1064, 1403
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 3 cut(s) 146, 1291, 1342
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 4 cut(s) 501, 900, 1107, 1390
BssMI GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
BssT1I CCWWGG 4 cut(s) 501, 900, 1107, 1390
Bst2UI CCWGG 3 cut(s) 873, 1074, 1182
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 1158, 1347
BstBAI YACGTR 1 cut(s) 350
BstDEI CTNAG 7 cut(s) 369, 576, 753, 1361, 1503, 1615, 1625
BstDSI CCRYGG 2 cut(s) 900, 1107
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 5 cut(s) 173, 653, 666, 982, 1378
BstKTI GATC 6 cut(s) 859, 1072, 1170, 1262, 1398, 1543
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
BstMWI GCNNNNNNNGC 2 cut(s) 587, 1040
BstNI CCWGG 3 cut(s) 873, 1074, 1182
BstSCI CCNGG 3 cut(s) 871, 1072, 1180
BstSFI CTRYAG 2 cut(s) 910, 1038
BstV1I GCAGC 2 cut(s) 880, 1052
BstV2I GAAGAC 2 cut(s) 838, 1602
BstX2I RGATCY 2 cut(s) 1167, 1259
BstYI RGATCY 2 cut(s) 1167, 1259
BsuRI GGCC 4 cut(s) 768, 1329, 1340, 1519
BtgI CCRYGG 2 cut(s) 900, 1107
BtsCI GGATG 5 cut(s) 173, 653, 666, 982, 1378
BtsIMutI CAGTG 4 cut(s) 298, 465, 750, 945
BveI ACCTGC 1 cut(s) 559
CciI TCATGA 1 cut(s) 1089
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 7 cut(s) 54, 384, 1328, 1339, 1387, 1412, 1517
Csp6I GTAC 3 cut(s) 745, 1345, 1358
CspCI CAANNNNNGTGG 2 cut(s) 1428, 1463
CviAII CATG 9 cut(s) 374, 655, 796, 901, 1090, 1108, 1255, 1402, 1571
CviQI GTAC 3 cut(s) 745, 1345, 1358
DdeI CTNAG 7 cut(s) 369, 576, 753, 1361, 1503, 1615, 1625
DpnI GATC 6 cut(s) 858, 1071, 1169, 1261, 1397, 1542
DpnII GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
Eam1104I CTCTTC 2 cut(s) 1158, 1347
EarI CTCTTC 2 cut(s) 1158, 1347
Eco130I CCWWGG 4 cut(s) 501, 900, 1107, 1390
Eco24I GRGCYC 1 cut(s) 108
Eco32I GATATC 1 cut(s) 1292
Eco47I GGWCC 4 cut(s) 54, 384, 1387, 1412
Eco57I CTGAAG 3 cut(s) 183, 985, 1614
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoO109I RGGNCCY 2 cut(s) 54, 1517
EcoRII CCWGG 3 cut(s) 871, 1072, 1180
EcoRV GATATC 1 cut(s) 1292
EcoT14I CCWWGG 4 cut(s) 501, 900, 1107, 1390
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 4 cut(s) 501, 900, 1107, 1390
FaeI CATG 9 cut(s) 377, 658, 799, 904, 1093, 1111, 1258, 1405, 1574
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 620, 652
FaqI GGGAC 2 cut(s) 558, 866
FatI CATG 9 cut(s) 373, 654, 795, 900, 1089, 1107, 1254, 1401, 1570
FbaI TGATCA 1 cut(s) 1540
Fnu4HI GCNGC 2 cut(s) 869, 1041
FokI GGATG 5 cut(s) 180, 653, 660, 989, 1365
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 2 cut(s) 869, 1041
FspBI CTAG 2 cut(s) 110, 948
GluI GCNGC 2 cut(s) 869, 1041
GsaI CCCAGC 1 cut(s) 880
HaeIII GGCC 4 cut(s) 768, 1329, 1340, 1519
HapII CCGG 1 cut(s) 336
Hin1II CATG 9 cut(s) 377, 658, 799, 904, 1093, 1111, 1258, 1405, 1574
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HindIII AAGCTT 2 cut(s) 114, 1627
HinfI GANTC 4 cut(s) 433, 930, 991, 1005
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 1 cut(s) 336
HphI GGTGA 6 cut(s) 368, 490, 571, 718, 811, 1597
Hpy166II GTNNAC 3 cut(s) 142, 475, 883
Hpy188I TCNGA 5 cut(s) 634, 647, 1004, 1197, 1674
Hpy188III TCNNGA 5 cut(s) 705, 948, 1090, 1476, 1523
Hpy8I GTNNAC 3 cut(s) 142, 475, 883
Hpy99I CGWCG 1 cut(s) 1127
HpyAV CCTTC 3 cut(s) 158, 232, 290
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 2 cut(s) 17, 349
HpyCH4V TGCA 9 cut(s) 12, 218, 568, 701, 788, 868, 1040, 1216, 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 1040
HpyF3I CTNAG 7 cut(s) 369, 576, 753, 1361, 1503, 1615, 1625
HpySE526I ACGT 2 cut(s) 17, 349
Hsp92II CATG 9 cut(s) 377, 658, 799, 904, 1093, 1111, 1258, 1405, 1574
Ksp22I TGATCA 1 cut(s) 1540
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 2 cut(s) 880, 1052
LweI GCATC 5 cut(s) 227, 710, 926, 1139, 1387
MaeI CTAG 2 cut(s) 110, 948
MaeII ACGT 2 cut(s) 17, 349
MaeIII GTNAC 7 cut(s) 40, 571, 836, 1220, 1585, 1603, 1636
MalI GATC 6 cut(s) 858, 1071, 1169, 1261, 1397, 1542
MboI GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
MboII GAAGA 6 cut(s) 838, 986, 1165, 1175, 1364, 1607
MflI RGATCY 2 cut(s) 1167, 1259
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 7 cut(s) 27, 412, 691, 803, 934, 966, 1609
MmeI TCCRAC 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 934
MseI TTAA 5 cut(s) 150, 324, 882, 1431, 1632
MslI CAYNNNNRTG 2 cut(s) 646, 1082
MspA1I CMGCKG 2 cut(s) 1424, 1592
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 3 cut(s) 873, 1074, 1182
Mva1269I GAATGC 1 cut(s) 1043
MvaI CCWGG 3 cut(s) 873, 1074, 1182
MwoI GCNNNNNNNGC 2 cut(s) 587, 1040
NcoI CCATGG 2 cut(s) 900, 1107
NdeII GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
NlaIII CATG 9 cut(s) 377, 658, 799, 904, 1093, 1111, 1258, 1405, 1574
NlaIV GGNNCC 2 cut(s) 386, 1518
NmuCI GTSAC 3 cut(s) 836, 1220, 1603
PagI TCATGA 1 cut(s) 1089
PctI GAATGC 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 934
PfeI GAWTC 4 cut(s) 433, 930, 991, 1005
PflFI GACNNNGTC 3 cut(s) 479, 628, 1601
PflMI CCANNNNNTGG 1 cut(s) 1348
PfoI TCCNGGA 1 cut(s) 1180
PkrI GCNGC 2 cut(s) 870, 1042
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 871, 1072, 1180
PspFI CCCAGC 1 cut(s) 876
PspGI CCWGG 3 cut(s) 871, 1072, 1180
PspN4I GGNNCC 2 cut(s) 386, 1518
PspPI GGNCC 7 cut(s) 54, 384, 1328, 1339, 1387, 1412, 1517
PspPPI RGGWCCY 1 cut(s) 54
PstI CTGCAG 1 cut(s) 1042
PsuI RGATCY 2 cut(s) 1167, 1259
PsyI GACNNNGTC 3 cut(s) 479, 628, 1601
PvuII CAGCTG 2 cut(s) 1424, 1592
RsaI GTAC 3 cut(s) 746, 1346, 1359
RsaNI GTAC 3 cut(s) 745, 1345, 1358
RseI CAYNNNNRTG 2 cut(s) 646, 1082
SaqAI TTAA 5 cut(s) 150, 324, 882, 1431, 1632
SatI GCNGC 2 cut(s) 869, 1041
Sau3AI GATC 6 cut(s) 856, 1069, 1167, 1259, 1395, 1540
Sau96I GGNCC 7 cut(s) 54, 384, 1328, 1339, 1387, 1412, 1517
ScaI AGTACT 1 cut(s) 1346
ScrFI CCNGG 3 cut(s) 873, 1074, 1182
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 5 cut(s) 227, 710, 926, 1139, 1387
SfcI CTRYAG 2 cut(s) 910, 1038
SgrAI CRCCGGYG 1 cut(s) 335
SinI GGWCC 4 cut(s) 54, 384, 1387, 1412
SmiMI CAYNNNNRTG 2 cut(s) 646, 1082
Sse9I AATT 7 cut(s) 27, 412, 691, 803, 934, 966, 1609
SsiI CCGC 1 cut(s) 1385
SspMI CTAG 2 cut(s) 110, 948
StyD4I CCNGG 3 cut(s) 871, 1072, 1180
StyI CCWWGG 4 cut(s) 501, 900, 1107, 1390
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 2 cut(s) 20, 352
TasI AATT 7 cut(s) 27, 412, 691, 803, 934, 966, 1609
TatI WGTACW 1 cut(s) 1344
TfiI GAWTC 4 cut(s) 433, 930, 991, 1005
Tru1I TTAA 5 cut(s) 150, 324, 882, 1431, 1632
Tru9I TTAA 5 cut(s) 150, 324, 882, 1431, 1632
TscAI CASTG 4 cut(s) 298, 465, 757, 945
TseFI GTSAC 3 cut(s) 836, 1220, 1603
TseI GCWGC 2 cut(s) 868, 1040
Tsp45I GTSAC 3 cut(s) 836, 1220, 1603
TspDTI ATGAA 8 cut(s) 671, 752, 998, 1078, 1106, 1449, 1559, 1662
TspRI CASTG 4 cut(s) 298, 465, 757, 945
Tth111I GACNNNGTC 3 cut(s) 479, 628, 1601
Van91I CCANNNNNTGG 1 cut(s) 1348
VpaK11BI GGWCC 4 cut(s) 54, 384, 1387, 1412
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 2 cut(s) 412, 803
XbaI TCTAGA 1 cut(s) 947
XmnI GAANNNNTTC 1 cut(s) 934
XspI CTAG 2 cut(s) 110, 948
ZrmI AGTACT 1 cut(s) 1346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.