RchiOBHm_Chr6g0252821

Glycosyl hydrolase family 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010160
Physical Location & Seq
Reverse (-)
7946021 .. 7948685
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22668

Sequence Viewer

Length: 1653 bp
ATGTTTAAAGAAGCTTGCACCAATCTATTGCTTGCCTGCATTTTTCTGTATTTTGGTTTTTGCTTGAAAGAGCCCCTAGAAGCTTTATATGGAGGAGGGATTATAGTTAACCCAGTTAATCACAACATTGAAGGATGGAAAGCATTTGGACAAGGAAAAATCAGAGAACGAATATCAAAAACTGCATCAAACAGTTTCTCGCAGAAGGTTCAAGTTGAGCAAGGGAAAATCTATACTCTTTCTGCTTCTGTCCAAGTCAGTGAAGGAAGTGAGATTGTAGCTGTTGTTTTTAAGTTCCCCACCGGCGAAAACATACGTGGTGGTGATGTGGTAGCTGAGCATGGTTGCTGGACCCTGCTAAAAGGTGGCATAGTAGCAAATTTCACAAGCTTGGTTGAGATTTTTTTTGAGAGCAAAAATACATTAGTGGAAATATGGGTGGACAATGTTTCGTTACAGCCATTCACCAAGAAGGAATGGAAATCACACCAAGACAAAAGCATTATTGAGGGACGAAAGAGTAAGGTGAGGTTGCAGGTAACTCAGGCAAATAAAGCTCCTCTTACAGGTGCTAAAGTGTCCATCAAGCAGACGAAGTCGGACTTCCCATTCGGATGTTGCATGAACCATAACATCCTCACAAGCACCGATTACCAGAATTGGTTTGCATCAAGATTTAGATATACCACTTTCACCAATGAGATGAAGTGGTACAGCACTGAGAAAATAGAAGGCCAAGAAAACTATACTATTGCAGATGGCATGGTGAAATTTTCCAAAGAGAATGGCATATCTATTAGAGGTCACAATGTCTTCTGGGATGATCCAAAAATGCAGCCTGGCTGGGTTAACAGTACCATTTCCCCCGAGAAACTACGAACAGCATCAGCACAGAGAATCAATTCAGTGGTTTCTAGATACAGAGGACAACTAATTGCTTGGGATGTAATGAATGAGAATCTTCACTTCCGATTCTTTGAGGATAAACTTGGCGAGAACGCTTCTGCAGCATTCTACTACACAGCTCAACAGCTTGATCCTGGCACAATGATGTTCATGAATGAGTATAATACCATGGAATATAGTGGCGACGAGGCATCAAGTCCAGCCAACTACAGGAAGAAACTTGAAGAGATCCTGTCATATCCTGGAAATGCTAATCTGACAGCAGGAATAGGTTTGCAAGGTCACTTTGGTTCTGGTCAGCCAAATCTTGCTTACATGAGATCTGTTTTAGACTTTTTAGGTGCAACTGGATATCCAATATGGATTACAGAAGTGAGTGTGGGAGTAGGCCCATATCAGGCCCAGTACTTGGAAGAGGTACTAAGGGAGGGTTATTCGCATCCTGCAGTCCAAGGGATCATCATGTTCGTAGGTCCAATCACAGCTGGTTTTAATGAAACCACTCTTGTAGATAAGAACTTCAACAACACTTCATCAGGAGATGTTGTGGATAAGCTGATTGCCGAGTGGAAAACGGGGACTCTGGAAATGACAACTGATGGTCAGGGATTTATAGACGTTTCCTTGTTTCATGGAGATTATGAGGTTACAGCTGAAGACAATGACACCAATTCCTCAGCTACACTAAGCTTTAAGGTCACAAAAGATGAACCACAGGCAAGTGTTCAAGTTCAGATTGATACTTGA

Protein Analysis

550

Amino Acids

61.4

Weight (kDa)

5.4

Isoelectric Point (pI)

26.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 198 - 448 4.6e-38 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14690 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33820
fragaria_vesca FvH4_2g03791
malus_domestica MD05G1100900.v1.1
prunus_persica Prupe.8G145600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252821
rosa_laevigata RLG00000015063
rosa_multiflora Rmu_co8501457.1_g000001
rosa_roxburghii Rroxscaffold_7G00212400
rosa_rugosa Rorug05G0538800 Rorug05G0538800
rosa_samantha Rh6AG054900 Rh6BG048900 Rh6CG047800 Rh6DG043300
rosa_wichuraiana Rw6G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 526
AccB7I CCANNNNNTGG 1 cut(s) 1315
AclWI GGATC 4 cut(s) 818, 1031, 1129, 1370
AcsI RAATTY 2 cut(s) 379, 770
AcuI CTGAAG 1 cut(s) 1581
AfaI GTAC 4 cut(s) 713, 856, 1313, 1326
AfiI CCNNNNNNNGG 4 cut(s) 566, 1116, 1303, 1315
AgsI TTSAA 6 cut(s) 67, 131, 212, 1130, 1429, 1634
AjnI CCWGG 3 cut(s) 838, 1039, 1147
AlwI GGATC 4 cut(s) 818, 1031, 1129, 1370
Ama87I CYCGRG 1 cut(s) 866
AoxI GGCC 3 cut(s) 733, 1294, 1305
ApeKI GCWGC 2 cut(s) 835, 1007
ApoI RAATTY 2 cut(s) 379, 770
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
Asp700I GAANNNNTTC 1 cut(s) 901
AspS9I GGNCC 4 cut(s) 351, 1295, 1306, 1379
AsuHPI GGTGA 5 cut(s) 335, 457, 538, 685, 778
AvaI CYCGRG 1 cut(s) 866
AvaII GGWCC 2 cut(s) 351, 1379
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 2 cut(s) 805, 1569
BbvCI CCTCAGC 1 cut(s) 1582
BbvI GCAGC 2 cut(s) 847, 1019
BccI CCATC 4 cut(s) 129, 590, 752, 1499
BciT130I CCWGG 3 cut(s) 840, 1041, 1149
BfaI CTAG 2 cut(s) 77, 915
BfmI CTRYAG 3 cut(s) 1005, 1114, 1350
BfuAI ACCTGC 1 cut(s) 526
BglII AGATCT 1 cut(s) 1226
BisI GCNGC 2 cut(s) 836, 1008
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 2 cut(s) 837, 1009
BmcAI AGTACT 1 cut(s) 1313
Bme1390I CCNGG 3 cut(s) 840, 1041, 1149
Bme18I GGWCC 2 cut(s) 351, 1379
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 4 cut(s) 351, 1295, 1306, 1379
BmiI GGNNCC 1 cut(s) 353
BmrFI CCNGG 3 cut(s) 840, 1041, 1149
BmrI ACTGGG 2 cut(s) 107, 1303
BmsI GCATC 5 cut(s) 194, 677, 893, 1106, 1354
BmuI ACTGGG 2 cut(s) 107, 1303
BpiI GAAGAC 2 cut(s) 805, 1569
Bpu10I CCTNAGC 1 cut(s) 1582
Bpu1102I GCTNAGC 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 1074, 1357
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 4 cut(s) 566, 1116, 1303, 1315
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 113, 1258, 1309
BseBI CCWGG 3 cut(s) 840, 1041, 1149
BseDI CCNNGG 2 cut(s) 1074, 1357
BseGI GGATG 6 cut(s) 140, 620, 633, 826, 949, 1345
BseLI CCNNNNNNNGG 4 cut(s) 566, 1116, 1303, 1315
BseMII CTCAG 4 cut(s) 327, 557, 711, 1596
BseNI ACTGG 3 cut(s) 113, 1258, 1309
BseRI GAGGAG 2 cut(s) 108, 549
BseXI GCAGC 2 cut(s) 847, 1019
BseYI CCCAGC 1 cut(s) 843
BshFI GGCC 3 cut(s) 735, 1296, 1307
BsiHKCI CYCGRG 1 cut(s) 866
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 2 cut(s) 525, 1498
BslI CCNNNNNNNGG 4 cut(s) 566, 1116, 1303, 1315
BsmFI GGGAC 2 cut(s) 525, 1498
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 3 cut(s) 735, 1296, 1307
BsoBI CYCGRG 1 cut(s) 866
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
Bsp1720I GCTNAGC 1 cut(s) 336
Bsp19I CCATGG 1 cut(s) 1074
BspANI GGCC 3 cut(s) 735, 1296, 1307
BspCNI CTCAG 4 cut(s) 328, 556, 712, 1595
BspHI TCATGA 1 cut(s) 1056
BspLI GGNNCC 1 cut(s) 353
BspMAI CTGCAG 2 cut(s) 1009, 1354
BspMI ACCTGC 1 cut(s) 526
BspPI GGATC 4 cut(s) 818, 1031, 1129, 1370
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 3 cut(s) 113, 1258, 1309
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 2 cut(s) 1074, 1357
BssMI GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
BssT1I CCWWGG 2 cut(s) 1074, 1357
Bst2UI CCWGG 3 cut(s) 840, 1041, 1149
Bst4CI ACNGT 2 cut(s) 194, 854
Bst6I CTCTTC 2 cut(s) 1125, 1314
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 16, 33, 37
BstDEI CTNAG 6 cut(s) 336, 543, 720, 1328, 1582, 1592
BstDSI CCRYGG 1 cut(s) 1074
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 6 cut(s) 140, 620, 633, 826, 949, 1345
BstKTI GATC 5 cut(s) 826, 1039, 1137, 1229, 1365
BstMBI GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
BstMWI GCNNNNNNNGC 2 cut(s) 554, 1007
BstNI CCWGG 3 cut(s) 840, 1041, 1149
BstSCI CCNGG 3 cut(s) 838, 1039, 1147
BstSFI CTRYAG 3 cut(s) 1005, 1114, 1350
BstV1I GCAGC 2 cut(s) 847, 1019
BstV2I GAAGAC 2 cut(s) 805, 1569
BstX2I RGATCY 2 cut(s) 1134, 1226
BstYI RGATCY 2 cut(s) 1134, 1226
BsuRI GGCC 3 cut(s) 735, 1296, 1307
BtgI CCRYGG 1 cut(s) 1074
BtsCI GGATG 6 cut(s) 140, 620, 633, 826, 949, 1345
BtsIMutI CAGTG 3 cut(s) 265, 717, 912
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 3 cut(s) 16, 33, 37
CciI TCATGA 1 cut(s) 1056
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 4 cut(s) 351, 1295, 1306, 1379
Csp6I GTAC 4 cut(s) 712, 855, 1312, 1325
CviAII CATG 8 cut(s) 341, 622, 763, 1057, 1075, 1222, 1369, 1538
CviQI GTAC 4 cut(s) 712, 855, 1312, 1325
DdeI CTNAG 6 cut(s) 336, 543, 720, 1328, 1582, 1592
DpnI GATC 5 cut(s) 825, 1038, 1136, 1228, 1364
DpnII GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
DraI TTTAAA 1 cut(s) 7
Eam1104I CTCTTC 2 cut(s) 1125, 1314
EarI CTCTTC 2 cut(s) 1125, 1314
Eco130I CCWWGG 2 cut(s) 1074, 1357
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 1259
Eco47I GGWCC 2 cut(s) 351, 1379
Eco57I CTGAAG 1 cut(s) 1581
Eco88I CYCGRG 1 cut(s) 866
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 3 cut(s) 838, 1039, 1147
EcoRV GATATC 1 cut(s) 1259
EcoT14I CCWWGG 2 cut(s) 1074, 1357
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 1074, 1357
FaeI CATG 8 cut(s) 344, 625, 766, 1060, 1078, 1225, 1372, 1541
FalI AAGNNNNNCTT 4 cut(s) 546, 578, 587, 619
FaqI GGGAC 2 cut(s) 525, 1498
FatI CATG 8 cut(s) 340, 621, 762, 1056, 1074, 1221, 1368, 1537
Fnu4HI GCNGC 2 cut(s) 836, 1008
FokI GGATG 6 cut(s) 147, 620, 627, 833, 956, 1332
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 836, 1008
FspBI CTAG 2 cut(s) 77, 915
GluI GCNGC 2 cut(s) 836, 1008
GsaI CCCAGC 1 cut(s) 847
HaeIII GGCC 3 cut(s) 735, 1296, 1307
HapII CCGG 1 cut(s) 303
Hin1II CATG 8 cut(s) 344, 625, 766, 1060, 1078, 1225, 1372, 1541
HincII GTYRAC 2 cut(s) 109, 850
HindII GTYRAC 2 cut(s) 109, 850
HindIII AAGCTT 4 cut(s) 12, 81, 388, 1594
HinfI GANTC 4 cut(s) 897, 958, 972, 1486
HpaI GTTAAC 2 cut(s) 109, 850
HpaII CCGG 1 cut(s) 303
HphI GGTGA 5 cut(s) 335, 457, 538, 685, 778
Hpy166II GTNNAC 3 cut(s) 109, 442, 850
Hpy188I TCNGA 6 cut(s) 164, 601, 614, 971, 1164, 1641
Hpy188III TCNNGA 5 cut(s) 672, 915, 1057, 1443, 1490
Hpy8I GTNNAC 3 cut(s) 109, 442, 850
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 5 cut(s) 125, 199, 257, 466, 725
HpyCH4III ACNGT 2 cut(s) 194, 854
HpyCH4IV ACGT 2 cut(s) 316, 1524
HpyF10VI GCNNNNNNNGC 2 cut(s) 554, 1007
HpyF3I CTNAG 6 cut(s) 336, 543, 720, 1328, 1582, 1592
HpySE526I ACGT 2 cut(s) 316, 1524
Hsp92II CATG 8 cut(s) 344, 625, 766, 1060, 1078, 1225, 1372, 1541
KspAI GTTAAC 2 cut(s) 109, 850
Kzo9I GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
LmnI GCTCC 1 cut(s) 562
Lsp1109I GCAGC 2 cut(s) 847, 1019
LweI GCATC 5 cut(s) 194, 677, 893, 1106, 1354
MaeI CTAG 2 cut(s) 77, 915
MaeII ACGT 2 cut(s) 316, 1524
MaeIII GTNAC 6 cut(s) 453, 538, 803, 1187, 1552, 1603
MalI GATC 5 cut(s) 825, 1038, 1136, 1228, 1364
MboI GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
MboII GAAGA 6 cut(s) 805, 953, 1132, 1142, 1331, 1574
MflI RGATCY 2 cut(s) 1134, 1226
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 6 cut(s) 379, 658, 770, 901, 933, 1576
MlyI GAGTC 1 cut(s) 1480
MmeI TCCRAC 1 cut(s) 579
MroXI GAANNNNTTC 1 cut(s) 901
MseI TTAA 7 cut(s) 6, 108, 117, 291, 849, 1398, 1599
MslI CAYNNNNRTG 2 cut(s) 613, 1049
MspA1I CMGCKG 2 cut(s) 1391, 1559
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 3 cut(s) 840, 1041, 1149
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 3 cut(s) 840, 1041, 1149
MwoI GCNNNNNNNGC 2 cut(s) 554, 1007
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
NlaIII CATG 8 cut(s) 344, 625, 766, 1060, 1078, 1225, 1372, 1541
NlaIV GGNNCC 1 cut(s) 353
NmeAIII GCCGAG 1 cut(s) 1495
NmuCI GTSAC 3 cut(s) 803, 1187, 1603
PagI TCATGA 1 cut(s) 1056
PctI GAATGC 1 cut(s) 1010
PdmI GAANNNNTTC 1 cut(s) 901
PfeI GAWTC 3 cut(s) 897, 958, 972
PflFI GACNNNGTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 1315
PfoI TCCNGGA 1 cut(s) 1147
PkrI GCNGC 2 cut(s) 837, 1009
PleI GAGTC 1 cut(s) 1480
PpsI GAGTC 1 cut(s) 1480
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 3 cut(s) 838, 1039, 1147
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 3 cut(s) 838, 1039, 1147
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 4 cut(s) 351, 1295, 1306, 1379
PstI CTGCAG 2 cut(s) 1009, 1354
PsuI RGATCY 2 cut(s) 1134, 1226
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 1391, 1559
RsaI GTAC 4 cut(s) 713, 856, 1313, 1326
RsaNI GTAC 4 cut(s) 712, 855, 1312, 1325
RseI CAYNNNNRTG 2 cut(s) 613, 1049
SaqAI TTAA 7 cut(s) 6, 108, 117, 291, 849, 1398, 1599
SatI GCNGC 2 cut(s) 836, 1008
Sau3AI GATC 5 cut(s) 823, 1036, 1134, 1226, 1362
Sau96I GGNCC 4 cut(s) 351, 1295, 1306, 1379
ScaI AGTACT 1 cut(s) 1313
SchI GAGTC 1 cut(s) 1480
ScrFI CCNGG 3 cut(s) 840, 1041, 1149
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 5 cut(s) 194, 677, 893, 1106, 1354
SfcI CTRYAG 3 cut(s) 1005, 1114, 1350
SgrAI CRCCGGYG 1 cut(s) 302
SinI GGWCC 2 cut(s) 351, 1379
SmiMI CAYNNNNRTG 2 cut(s) 613, 1049
Sse9I AATT 6 cut(s) 379, 658, 770, 901, 933, 1576
SspMI CTAG 2 cut(s) 77, 915
StyD4I CCNGG 3 cut(s) 838, 1039, 1147
StyI CCWWGG 2 cut(s) 1074, 1357
TaaI ACNGT 2 cut(s) 194, 854
TaiI ACGT 2 cut(s) 319, 1527
TasI AATT 6 cut(s) 379, 658, 770, 901, 933, 1576
TatI WGTACW 1 cut(s) 1311
TfiI GAWTC 3 cut(s) 897, 958, 972
Tru1I TTAA 7 cut(s) 6, 108, 117, 291, 849, 1398, 1599
Tru9I TTAA 7 cut(s) 6, 108, 117, 291, 849, 1398, 1599
TscAI CASTG 3 cut(s) 265, 724, 912
TseFI GTSAC 3 cut(s) 803, 1187, 1603
TseI GCWGC 2 cut(s) 835, 1007
Tsp45I GTSAC 3 cut(s) 803, 1187, 1603
TspDTI ATGAA 9 cut(s) 638, 719, 965, 1045, 1073, 1416, 1428, 1526, 1629
TspRI CASTG 3 cut(s) 265, 724, 912
Tth111I GACNNNGTC 1 cut(s) 595
Van91I CCANNNNNTGG 1 cut(s) 1315
VpaK11BI GGWCC 2 cut(s) 351, 1379
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 2 cut(s) 379, 770
XbaI TCTAGA 1 cut(s) 914
XmnI GAANNNNTTC 1 cut(s) 901
XspI CTAG 2 cut(s) 77, 915
ZrmI AGTACT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.