Rroxscaffold_7G00212400

Glycosyl hydrolase family 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010160
Physical Location & Seq
Forward (+)
62881975 .. 62884754
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212400.1

Sequence Viewer

Length: 726 bp
ATGAACCATAACATCCTCACAAGCACCGATTACCAGAATTGGTTTGCATCAAGATTTAGATATACCACTTTCACCAATGAGATGAAGTGGTACAGCACTGAGAAAATAGAAGACCAAGAAAACTATACTATTGCAGATGGCATGGTGAAATTTTCCAAAGAGAATGGCATATCTATTAGAGGTCACAATGTCTTCTGGGATGATCCAAAAATGCAGCCTGGCTGGGTTAACAGTACCATTTCCCCCGAGAAACTACAAACAGCATCAGCACAGAGAATCAATTCAGTGGTTTCTAGATACAGTGGACAACTAATTGCTTGGGATGTAATGAATGAGAATCTTCACTTCCGATTCTTTGAGGATAAACTTGGCGAGAACGCTTCTGCAGAATTCTACTTCACAGCTCAACAGCTTGATCCTGGCACAATGATGTTCATGAATGAGTATAATACCATGGAATATAGTGGCGACGAAGCATCAAGTCCAGCCAACTACAAGAAGAAACTTGAAGAGATCCTGTCATATCCTGGAAATGCTAATCTGACAGCAGGAATAGGTTTGCAAGGTCACTTTGGTTCTGGTCAGCCAAATCTTGCTTACATGAGATCTGTTTTAGACTTTTTAGGTGCAACTGGATATCCAATATGGCTTACAGAAGTGAGTGTGGGAGTAGGCCCATATCAGGTAATTCTAATAATTTGTTTTAAGCAATACTACAATATGTAA

Protein Analysis

241

Amino Acids

27.6

Weight (kDa)

5.02

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 4 - 222 1.3e-34 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14690 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33810 AT4G33820
fragaria_vesca FvH4_2g03791
malus_domestica MD05G1100900.v1.1
prunus_persica Prupe.8G145600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252821
rosa_laevigata RLG00000015063
rosa_multiflora Rmu_co8501457.1_g000001
rosa_roxburghii Rroxscaffold_7G00212400
rosa_rugosa Rorug05G0538800 Rorug05G0538800
rosa_samantha Rh6AG054900 Rh6BG048900 Rh6CG047800 Rh6DG043300
rosa_wichuraiana Rw6G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 197, 410, 508
AcsI RAATTY 2 cut(s) 149, 389
AfaI GTAC 2 cut(s) 92, 235
AfiI CCNNNNNNNGG 1 cut(s) 682
AgsI TTSAA 1 cut(s) 509
AjnI CCWGG 3 cut(s) 217, 418, 526
AluBI AGCT 2 cut(s) 404, 412
AluI AGCT 2 cut(s) 404, 412
AlwI GGATC 3 cut(s) 197, 410, 508
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 1 cut(s) 673
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 2 cut(s) 149, 389
Asp700I GAANNNNTTC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 2 cut(s) 64, 157
AvaI CYCGRG 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 117, 184
BbvI GCAGC 1 cut(s) 226
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 3 cut(s) 219, 420, 528
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 1 cut(s) 384
BglII AGATCT 1 cut(s) 605
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
Bme1390I CCNGG 3 cut(s) 219, 420, 528
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 674
BmrFI CCNGG 3 cut(s) 219, 420, 528
BmsI GCATC 3 cut(s) 56, 272, 485
BpiI GAAGAC 2 cut(s) 117, 184
BsaJI CCNNGG 1 cut(s) 453
Bsc4I CCNNNNNNNGG 1 cut(s) 682
Bse1I ACTGG 1 cut(s) 637
BseBI CCWGG 3 cut(s) 219, 420, 528
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 12, 205, 328
BseLI CCNNNNNNNGG 1 cut(s) 682
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 637
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 1 cut(s) 675
BsiHKCI CYCGRG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 682
BsnI GGCC 1 cut(s) 675
BsoBI CYCGRG 1 cut(s) 245
Bsp143I GATC 4 cut(s) 202, 415, 513, 605
Bsp19I CCATGG 1 cut(s) 453
BspANI GGCC 1 cut(s) 675
BspCNI CTCAG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 3 cut(s) 197, 410, 508
BsrI ACTGG 1 cut(s) 637
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 4 cut(s) 202, 415, 513, 605
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 219, 420, 528
Bst4CI ACNGT 2 cut(s) 233, 302
Bst6I CTCTTC 1 cut(s) 504
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 3 cut(s) 12, 205, 328
BstKTI GATC 4 cut(s) 205, 418, 516, 608
BstMBI GATC 4 cut(s) 202, 415, 513, 605
BstNI CCWGG 3 cut(s) 219, 420, 528
BstSCI CCNGG 3 cut(s) 217, 418, 526
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 226
BstV2I GAAGAC 2 cut(s) 117, 184
BstX2I RGATCY 2 cut(s) 513, 605
BstYI RGATCY 2 cut(s) 513, 605
BsuRI GGCC 1 cut(s) 675
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 3 cut(s) 12, 205, 328
BtsIMutI CAGTG 3 cut(s) 96, 291, 307
CciI TCATGA 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 674
Csp6I GTAC 2 cut(s) 91, 234
CviAII CATG 4 cut(s) 142, 436, 454, 601
CviJI RGCY 8 cut(s) 217, 222, 404, 412, 488, 586, 649, 675
CviKI_1 RGCY 8 cut(s) 217, 222, 404, 412, 488, 586, 649, 675
CviQI GTAC 2 cut(s) 91, 234
DdeI CTNAG 1 cut(s) 99
DpnI GATC 4 cut(s) 204, 417, 515, 607
DpnII GATC 4 cut(s) 202, 415, 513, 605
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 453
Eco32I GATATC 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 3 cut(s) 217, 418, 526
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 4 cut(s) 145, 439, 457, 604
FatI CATG 4 cut(s) 141, 435, 453, 600
Fnu4HI GCNGC 1 cut(s) 215
FokI GGATG 2 cut(s) 212, 335
Fsp4HI GCNGC 1 cut(s) 215
FspBI CTAG 1 cut(s) 294
GluI GCNGC 1 cut(s) 215
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 1 cut(s) 675
Hin1II CATG 4 cut(s) 145, 439, 457, 604
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 3 cut(s) 276, 337, 351
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 2 cut(s) 64, 157
Hpy166II GTNNAC 2 cut(s) 229, 305
Hpy188I TCNGA 2 cut(s) 350, 543
Hpy188III TCNNGA 3 cut(s) 51, 294, 436
Hpy8I GTNNAC 2 cut(s) 229, 305
Hpy99I CGWCG 1 cut(s) 473
HpyCH4III ACNGT 2 cut(s) 233, 302
HpyCH4V TGCA 6 cut(s) 47, 134, 214, 386, 562, 629
HpyF3I CTNAG 1 cut(s) 99
Hsp92II CATG 4 cut(s) 145, 439, 457, 604
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 202, 415, 513, 605
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 3 cut(s) 56, 272, 485
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 182, 566
MalI GATC 4 cut(s) 204, 417, 515, 607
MboI GATC 4 cut(s) 202, 415, 513, 605
MboII GAAGA 5 cut(s) 122, 184, 332, 511, 521
MflI RGATCY 2 cut(s) 513, 605
MluCI AATT 7 cut(s) 37, 149, 280, 312, 389, 687, 696
MnlI CCTC 3 cut(s) 26, 173, 352
MroXI GAANNNNTTC 1 cut(s) 280
MseI TTAA 2 cut(s) 228, 705
MslI CAYNNNNRTG 1 cut(s) 428
MspR9I CCNGG 3 cut(s) 219, 420, 528
MvaI CCWGG 3 cut(s) 219, 420, 528
NcoI CCATGG 1 cut(s) 453
NdeII GATC 4 cut(s) 202, 415, 513, 605
NlaIII CATG 4 cut(s) 145, 439, 457, 604
NmuCI GTSAC 2 cut(s) 182, 566
PagI TCATGA 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 3 cut(s) 276, 337, 351
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 1 cut(s) 216
Psp6I CCWGG 3 cut(s) 217, 418, 526
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 3 cut(s) 217, 418, 526
PspPI GGNCC 1 cut(s) 674
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 513, 605
RsaI GTAC 2 cut(s) 92, 235
RsaNI GTAC 2 cut(s) 91, 234
RseI CAYNNNNRTG 1 cut(s) 428
SaqAI TTAA 2 cut(s) 228, 705
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 4 cut(s) 202, 415, 513, 605
Sau96I GGNCC 1 cut(s) 674
ScrFI CCNGG 3 cut(s) 219, 420, 528
SetI ASST 7 cut(s) 184, 406, 414, 559, 568, 628, 687
SfaNI GCATC 3 cut(s) 56, 272, 485
SfcI CTRYAG 1 cut(s) 384
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 7 cut(s) 37, 149, 280, 312, 389, 687, 696
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 3 cut(s) 217, 418, 526
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 233, 302
TasI AATT 7 cut(s) 37, 149, 280, 312, 389, 687, 696
TfiI GAWTC 3 cut(s) 276, 337, 351
Tru1I TTAA 2 cut(s) 228, 705
Tru9I TTAA 2 cut(s) 228, 705
TscAI CASTG 3 cut(s) 103, 291, 307
TseFI GTSAC 2 cut(s) 182, 566
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 2 cut(s) 182, 566
TspDTI ATGAA 5 cut(s) 17, 98, 344, 424, 452
TspRI CASTG 3 cut(s) 103, 291, 307
XapI RAATTY 2 cut(s) 149, 389
XbaI TCTAGA 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.