MD05G1102000.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007574
Physical Location & Seq
Forward (+)
21054635 .. 21059839
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1102000.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGCTGGCGGCGGAGGAGGCCCCCAAAAGACAGATGACTTCCAGCCTCACCCCATCAAGGACCAGCTTCCCGGTGTCGACTTCTGTGTCACATCCTCTCCCCCATGGCCTGAAGCCATACTTCTGGGATTCCAGCATTTCCTGGTAATGCTTGGGACCACTGTGTTCATCCCCACATTACTTGTCCCTCTCATGGGTGGTGGCAATGTGGAGAAGGCTAAAGTGATTGAAACAATCCTCTTTGTTGCTGGGATCAACACACTCTTGCAGACTTGGTTCGGGACTCGGCTTCCAGTGGTCATGGGGGCCTCATATGCCTTCATCATCCCTGTTGTCTCCATTGCCTTGTCCAGGAGGTTCGATGTCTACATAGACCCCCACCGGAGGTTTAAGGAAACCATGAAAGCAGTGCAAGGAGCAATTGTGATTGCATCTCTCTTGCAGGTGATCTTTGGTTTTCTTGGGTTTGTGAGAATTTTTGGGAGGTATCTTAGTCCTCTCTCTGCAGTTCCTCTTGTAACGCTTACTGGACTTGGGTTCTTTGCATTTGGTTTCCCTCAGCTGGCAAAATGTGTTGAAATTGGATTACCAGCACTGATCCTTGTGGTTTTCATATCACAGTACACGATGAGGTTAAAACCGCCTATATTTGGTCGATTTGCCGTACTGTTTACAGTTGCGATTGTATGGACATATGCAGAAATTTTGACAGCAGCTGGTGCGTATAAGAAAAGATCTTATGCAACTCAAATTAGTTGCCGCACTGATCGTTCAGGGCTGATTAGCGCTGCTCCTTGGATAAGGATTCCCTATCCGTTTCAATGGGGGCGTCCTGATTTTAATGCTGGAGATATTTTTGCAATGATGGCTGCGGCTTTCGTTGCCATTATTGAGTCTACAGGTACATTTATTGCGGCGTCAAGATATGGGAGTGCCACCCCTATACCACCTTCTGTTATCAGCAGAGGTGTTGGCTGGCTGGGCGTAAGCACTTTTCTGGATGGAATATTTGGCTCAGTATCTGGCTCCACTGCTTCAGTTGAAAATGCTGGCCTTCTGGGAATGACACGGGTTGGAAGTCGGAGAGTAATTCAGATATCAGCAGCCTTTATGCTCTTCTTCTCTGTATTAGGAAAATTTGGGGCTTTTCTTGCTTCGATACCAATGCCAATTTTCGCAGCGTTGTATTGCGTCCTCTTCGCCTATGTTGCTTCTGCTGGCCTTGGTTTGCTCCAGTTCTGCAACCTAAACAGCTTTAGGACGATGTTCATTGTAGGCTTTTCTATCTTCATGGGTCTTTCGGTGACAGAATACTTTCATGAATACCACTTGATTTCTGATCGCGGTCCTGTTCACACTCGTTCCATTTGGTTCAACAATATCGTTGAAGTAATTTTCTCGTCTCCAGCAACCGTTGGCATCATTGTCGCTTTGTTCTTGGACTTAACTGTCAGCCGTGGACACAGCACAACTCGGAGAGACAGTGGTAGGCACTGGTGGGAAAAATTTCAGAATTACAACACAGATACCAGGAGTGAAGAGTTCTACTCACTGCCCTACAACCTCAATAGGCATTTCCCTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

530

Amino Acids

58.04

Weight (kDa)

9.53

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 39 - 439 1.4e-64 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 433
AasI GACNNNNNNGTC 2 cut(s) 86, 1448
Acc36I ACCTGC 1 cut(s) 433
AccI GTMKAC 3 cut(s) 78, 366, 896
AccII CGCG 1 cut(s) 1344
AciI CCGC 7 cut(s) 9, 12, 641, 760, 872, 914, 1344
AclWI GGATC 2 cut(s) 260, 592
AcsI RAATTY 4 cut(s) 474, 702, 1136, 1505
AcuI CTGAAG 3 cut(s) 132, 1020, 1566
AcyI GRCGYC 2 cut(s) 829, 917
AfaI GTAC 3 cut(s) 623, 666, 904
AfeI AGCGCT 1 cut(s) 787
AfiI CCNNNNNNNGG 7 cut(s) 58, 193, 194, 351, 384, 562, 650
AgsI TTSAA 6 cut(s) 230, 578, 821, 1043, 1375, 1388
AjnI CCWGG 3 cut(s) 141, 350, 1529
AluBI AGCT 4 cut(s) 67, 562, 716, 1254
AluI AGCT 4 cut(s) 67, 562, 716, 1254
Alw26I GTCTC 3 cut(s) 340, 1407, 1473
AlwI GGATC 2 cut(s) 260, 592
AlwNI CAGNNNCTG 2 cut(s) 716, 1022
Aor51HI AGCGCT 1 cut(s) 787
AoxI GGCC 5 cut(s) 19, 107, 306, 1051, 1219
ApeKI GCWGC 5 cut(s) 713, 788, 869, 1103, 1178
ApoI RAATTY 4 cut(s) 474, 702, 1136, 1505
Asp700I GAANNNNTTC 2 cut(s) 1314, 1506
AspLEI GCGC 1 cut(s) 788
AspS9I GGNCC 5 cut(s) 20, 61, 156, 306, 1346
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 41, 457, 1315
AvaII GGWCC 3 cut(s) 61, 156, 1346
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 5 cut(s) 725, 775, 856, 1115, 1190
BccI CCATC 3 cut(s) 62, 859, 995
BceAI ACGGC 2 cut(s) 647, 1440
BcgI CGANNNNNNTGC 4 cut(s) 1147, 1181, 1408, 1442
BciT130I CCWGG 3 cut(s) 143, 352, 1531
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 3 cut(s) 340, 1407, 1473
BfmI CTRYAG 2 cut(s) 504, 897
BfoI RGCGCY 1 cut(s) 789
BfuAI ACCTGC 1 cut(s) 433
BglII AGATCT 1 cut(s) 734
BisI GCNGC 9 cut(s) 10, 714, 760, 789, 870, 873, 915, 1104, 1179
BlsI GCNGC 9 cut(s) 11, 715, 761, 790, 871, 874, 916, 1105, 1180
Bme1390I CCNGG 4 cut(s) 72, 143, 352, 1531
Bme18I GGWCC 3 cut(s) 61, 156, 1346
BmgT120I GGNCC 5 cut(s) 20, 61, 156, 306, 1346
BmiI GGNNCC 4 cut(s) 22, 157, 307, 1027
BmrFI CCNGG 4 cut(s) 72, 143, 352, 1531
BmsI GCATC 2 cut(s) 440, 1428
BplI GAGNNNNNCTC 2 cut(s) 1532, 1564
BpmI CTGGAG 3 cut(s) 867, 1217, 1389
Bpu10I CCTNAGC 1 cut(s) 558
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 2 cut(s) 829, 917
BsaJI CCNNGG 4 cut(s) 104, 794, 1222, 1456
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 6 cut(s) 82, 112, 408, 438, 1468, 1498
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 193, 194, 351, 384, 562, 650
Bse1I ACTGG 4 cut(s) 293, 532, 1234, 1499
Bse3DI GCAATG 3 cut(s) 211, 339, 867
BseBI CCWGG 3 cut(s) 143, 352, 1531
BseDI CCNNGG 4 cut(s) 104, 794, 1222, 1456
BseGI GGATG 4 cut(s) 92, 168, 324, 1006
BseLI CCNNNNNNNGG 7 cut(s) 58, 193, 194, 351, 384, 562, 650
BseMI GCAATG 3 cut(s) 211, 339, 867
BseMII CTCAG 2 cut(s) 572, 1029
BseNI ACTGG 4 cut(s) 293, 532, 1234, 1499
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 5 cut(s) 725, 775, 856, 1115, 1190
BseYI CCCAGC 2 cut(s) 248, 979
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 5 cut(s) 21, 109, 308, 1053, 1221
BsiSI CCGG 2 cut(s) 72, 382
BslFI GGGAC 3 cut(s) 169, 170, 295
BslI CCNNNNNNNGG 7 cut(s) 58, 193, 194, 351, 384, 562, 650
BsmAI GTCTC 3 cut(s) 340, 1407, 1473
BsmBI CGTCTC 1 cut(s) 1407
BsmFI GGGAC 3 cut(s) 169, 170, 295
BsnI GGCC 5 cut(s) 21, 109, 308, 1053, 1221
Bsp143I GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 7 cut(s) 9, 12, 641, 760, 872, 914, 1344
BspANI GGCC 5 cut(s) 21, 109, 308, 1053, 1221
BspCNI CTCAG 2 cut(s) 571, 1028
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 4 cut(s) 22, 157, 307, 1027
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 2 cut(s) 260, 592
BspQI GCTCTTC 1 cut(s) 1121
BsrDI GCAATG 3 cut(s) 211, 339, 867
BsrI ACTGG 4 cut(s) 293, 532, 1234, 1499
BssECI CCNNGG 4 cut(s) 104, 794, 1222, 1456
BssMI GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
BssNI GRCGYC 2 cut(s) 829, 917
BssT1I CCWWGG 3 cut(s) 104, 794, 1222
Bst2UI CCWGG 3 cut(s) 143, 352, 1531
Bst4CI ACNGT 7 cut(s) 163, 621, 669, 676, 1414, 1450, 1484
Bst6I CTCTTC 3 cut(s) 1121, 1202, 1533
BstACI GRCGYC 2 cut(s) 829, 917
BstAPI GCANNNNNTGC 1 cut(s) 719
BstC8I GCNNGC 5 cut(s) 7, 564, 977, 1051, 1219
BstDEI CTNAG 3 cut(s) 491, 558, 1015
BstDSI CCRYGG 2 cut(s) 104, 1456
BstENI CCTNNNNNAGG 1 cut(s) 349
BstF5I GGATG 4 cut(s) 92, 168, 324, 1006
BstFNI CGCG 1 cut(s) 1344
BstH2I RGCGCY 1 cut(s) 789
BstHHI GCGC 1 cut(s) 788
BstKTI GATC 6 cut(s) 255, 450, 600, 737, 769, 1342
BstMAI GTCTC 3 cut(s) 340, 1407, 1473
BstMBI GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
BstMWI GCNNNNNNNGC 8 cut(s) 18, 314, 719, 866, 881, 981, 1151, 1208
BstNI CCWGG 3 cut(s) 143, 352, 1531
BstSCI CCNGG 4 cut(s) 70, 141, 350, 1529
BstSFI CTRYAG 2 cut(s) 504, 897
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 5 cut(s) 725, 775, 856, 1115, 1190
BstX2I RGATCY 1 cut(s) 734
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 1 cut(s) 734
BsuRI GGCC 5 cut(s) 21, 109, 308, 1053, 1221
BtgI CCRYGG 2 cut(s) 104, 1456
BtsCI GGATG 4 cut(s) 92, 168, 324, 1006
BtsI GCAGTG 3 cut(s) 414, 1029, 1550
BtsIMutI CAGTG 9 cut(s) 159, 300, 414, 593, 762, 1029, 1489, 1492, 1550
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 5 cut(s) 7, 564, 977, 1051, 1219
CaiI CAGNNNCTG 2 cut(s) 716, 1022
CciI TCATGA 1 cut(s) 1318
CfoI GCGC 1 cut(s) 788
Cfr13I GGNCC 5 cut(s) 20, 61, 156, 306, 1346
CseI GACGC 3 cut(s) 818, 906, 1180
Csp6I GTAC 3 cut(s) 622, 665, 903
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 6 cut(s) 105, 193, 301, 400, 1291, 1319
CviQI GTAC 3 cut(s) 622, 665, 903
DdeI CTNAG 3 cut(s) 491, 558, 1015
DpnI GATC 6 cut(s) 254, 449, 599, 736, 768, 1341
DpnII GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
DrdI GACNNNNNNGTC 2 cut(s) 86, 1448
DseDI GACNNNNNNGTC 2 cut(s) 86, 1448
Eam1104I CTCTTC 3 cut(s) 1121, 1202, 1533
EarI CTCTTC 3 cut(s) 1121, 1202, 1533
EciI GGCGGA 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 104, 794, 1222
Eco32I GATATC 1 cut(s) 1098
Eco47I GGWCC 3 cut(s) 61, 156, 1346
Eco47III AGCGCT 1 cut(s) 787
Eco57I CTGAAG 3 cut(s) 132, 1020, 1566
EcoNI CCTNNNNNAGG 1 cut(s) 349
EcoO109I RGGNCCY 2 cut(s) 20, 306
EcoRII CCWGG 3 cut(s) 141, 350, 1529
EcoRV GATATC 1 cut(s) 1098
EcoT14I CCWWGG 3 cut(s) 104, 794, 1222
ErhI CCWWGG 3 cut(s) 104, 794, 1222
Esp3I CGTCTC 1 cut(s) 1407
FaeI CATG 6 cut(s) 108, 196, 304, 403, 1294, 1322
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 3 cut(s) 169, 170, 295
FatI CATG 6 cut(s) 104, 192, 300, 399, 1290, 1318
FauNDI CATATG 2 cut(s) 313, 694
FblI GTMKAC 3 cut(s) 78, 366, 896
Fnu4HI GCNGC 9 cut(s) 10, 714, 760, 789, 870, 873, 915, 1104, 1179
FokI GGATG 4 cut(s) 79, 155, 311, 1013
Fsp4HI GCNGC 9 cut(s) 10, 714, 760, 789, 870, 873, 915, 1104, 1179
GlaI GCGC 1 cut(s) 787
GluI GCNGC 9 cut(s) 10, 714, 760, 789, 870, 873, 915, 1104, 1179
GsaI CCCAGC 2 cut(s) 252, 983
GsuI CTGGAG 3 cut(s) 867, 1217, 1389
HaeII RGCGCY 1 cut(s) 789
HaeIII GGCC 5 cut(s) 21, 109, 308, 1053, 1221
HapII CCGG 2 cut(s) 72, 382
HgaI GACGC 3 cut(s) 818, 906, 1180
HhaI GCGC 1 cut(s) 788
Hin1I GRCGYC 2 cut(s) 829, 917
Hin1II CATG 6 cut(s) 108, 196, 304, 403, 1294, 1322
Hin6I GCGC 1 cut(s) 786
HinP1I GCGC 1 cut(s) 786
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 4 cut(s) 129, 283, 805, 893
HpaII CCGG 2 cut(s) 72, 382
HphI GGTGA 3 cut(s) 41, 457, 1315
Hpy166II GTNNAC 7 cut(s) 79, 367, 624, 672, 897, 1354, 1460
Hpy188I TCNGA 5 cut(s) 1083, 1095, 1339, 1476, 1512
Hpy188III TCNNGA 5 cut(s) 280, 833, 921, 998, 1319
Hpy8I GTNNAC 7 cut(s) 79, 367, 624, 672, 897, 1354, 1460
HpyAV CCTTC 5 cut(s) 208, 328, 960, 1064, 1590
HpyCH4III ACNGT 7 cut(s) 163, 621, 669, 676, 1414, 1450, 1484
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 314, 719, 866, 881, 981, 1151, 1208
HpyF3I CTNAG 3 cut(s) 491, 558, 1015
Hsp92I GRCGYC 2 cut(s) 829, 917
Hsp92II CATG 6 cut(s) 108, 196, 304, 403, 1294, 1322
HspAI GCGC 1 cut(s) 786
Kzo9I GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
LguI GCTCTTC 1 cut(s) 1121
LmnI GCTCC 4 cut(s) 416, 796, 1031, 1236
Lsp1109I GCAGC 5 cut(s) 725, 775, 856, 1115, 1190
LweI GCATC 2 cut(s) 440, 1428
MaeIII GTNAC 3 cut(s) 88, 517, 1303
MalI GATC 6 cut(s) 254, 449, 599, 736, 768, 1341
MboI GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
MboII GAAGA 5 cut(s) 1108, 1111, 1189, 1279, 1550
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 1 cut(s) 734
MlyI GAGTC 2 cut(s) 277, 902
MmeI TCCRAC 2 cut(s) 1054, 1061
MroXI GAANNNNTTC 2 cut(s) 1314, 1506
MseI TTAA 4 cut(s) 390, 635, 840, 1445
MspA1I CMGCKG 2 cut(s) 562, 716
MspI CCGG 2 cut(s) 72, 382
MspR9I CCNGG 4 cut(s) 72, 143, 352, 1531
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 3 cut(s) 143, 352, 1531
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 8 cut(s) 18, 314, 719, 866, 881, 981, 1151, 1208
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 1 cut(s) 104
NdeI CATATG 2 cut(s) 313, 694
NdeII GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
NlaIII CATG 6 cut(s) 108, 196, 304, 403, 1294, 1322
NlaIV GGNNCC 4 cut(s) 22, 157, 307, 1027
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 88, 1303
PagI TCATGA 1 cut(s) 1318
PaqCI CACCTGC 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 1121
PdmI GAANNNNTTC 2 cut(s) 1314, 1506
PfeI GAWTC 2 cut(s) 129, 805
PfoI TCCNGGA 1 cut(s) 350
PkrI GCNGC 9 cut(s) 11, 715, 761, 790, 871, 874, 916, 1105, 1180
PleI GAGTC 2 cut(s) 277, 901
PpsI GAGTC 2 cut(s) 277, 901
Psp6I CCWGG 3 cut(s) 141, 350, 1529
PspFI CCCAGC 2 cut(s) 248, 979
PspGI CCWGG 3 cut(s) 141, 350, 1529
PspN4I GGNNCC 4 cut(s) 22, 157, 307, 1027
PspPI GGNCC 5 cut(s) 20, 61, 156, 306, 1346
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 2 cut(s) 716, 1022
PsuI RGATCY 1 cut(s) 734
PvuII CAGCTG 2 cut(s) 562, 716
RsaI GTAC 3 cut(s) 623, 666, 904
RsaNI GTAC 3 cut(s) 622, 665, 903
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 1121
SaqAI TTAA 4 cut(s) 390, 635, 840, 1445
SatI GCNGC 9 cut(s) 10, 714, 760, 789, 870, 873, 915, 1104, 1179
Sau3AI GATC 6 cut(s) 252, 447, 597, 734, 766, 1339
Sau96I GGNCC 5 cut(s) 20, 61, 156, 306, 1346
SchI GAGTC 2 cut(s) 277, 902
ScrFI CCNGG 4 cut(s) 72, 143, 352, 1531
SfaNI GCATC 2 cut(s) 440, 1428
SfcI CTRYAG 2 cut(s) 504, 897
SinI GGWCC 3 cut(s) 61, 156, 1346
SsiI CCGC 7 cut(s) 9, 12, 641, 760, 872, 914, 1344
SspI AATATT 1 cut(s) 1008
StyD4I CCNGG 4 cut(s) 70, 141, 350, 1529
StyI CCWWGG 3 cut(s) 104, 794, 1222
TaaI ACNGT 7 cut(s) 163, 621, 669, 676, 1414, 1450, 1484
TaqI TCGA 4 cut(s) 78, 360, 655, 1157
TatI WGTACW 1 cut(s) 621
TauI GCSGC 4 cut(s) 12, 762, 875, 917
TfiI GAWTC 2 cut(s) 129, 805
Tru1I TTAA 4 cut(s) 390, 635, 840, 1445
Tru9I TTAA 4 cut(s) 390, 635, 840, 1445
TscAI CASTG 9 cut(s) 166, 300, 414, 600, 769, 1036, 1489, 1499, 1557
TseFI GTSAC 2 cut(s) 88, 1303
TseI GCWGC 5 cut(s) 713, 788, 869, 1103, 1178
Tsp45I GTSAC 2 cut(s) 88, 1303
TspDTI ATGAA 8 cut(s) 157, 310, 416, 601, 1258, 1279, 1307, 1335
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 9 cut(s) 166, 300, 414, 600, 769, 1036, 1489, 1499, 1557
VpaK11BI GGWCC 3 cut(s) 61, 156, 1346
XagI CCTNNNNNAGG 1 cut(s) 349
XapI RAATTY 4 cut(s) 474, 702, 1136, 1505
XmiI GTMKAC 3 cut(s) 78, 366, 896
XmnI GAANNNNTTC 2 cut(s) 1314, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.