MD05G1118700.v1.1

Thaumatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006096
Physical Location & Seq
Forward (+)
23989552 .. 23993027
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1118700.v1.1.491

Sequence Viewer

Length: 825 bp
ATGGATCGTCCTCTACTCTCTGCCTCTCTTGTCGCTCTCGTCGCCCTCTCTTTCTTCTCAGAGGTGAACTCAGCTTCTTTCAAACTAGTCAACAAGTGCCGCTACGAAGTATGGCCCGGAATACTTTCCGGCGCCAACACGGCTCCGCTCTCCCCCACTGGCTTCGCGCTCAAACCCGGCAAGTCCCGGACCCTTTCCGTACCCAAATCTTGGTCGGGTCGGCTCTGGGCTCGGACTCTATGCACCGGATACTCGCCCGGTAACTTCTCCTGCGTCACAGCCGATTGCGGCTCGGGAAAAATCGAGTGTGCCGGAGGTGGCGCGAAACCGCCGGCTACGTTGGCCGAGTTCACGCTCAACGGCACCAACGGATTGGATTTTTATGACGTCAGCTTGGTGGACGGGTACAACTTGCCGATGCTGGTGGTGCCACGTGGAGGTACAAGAGGGGGATGCAGTCCGACTGGGTGCCTCGTCGACTTGAACGGCAAGTGCCCCAGGGCGCTGAGGGTGCGTGAGAGCGGAAGGGGGAGCGTGGCGTGTAGGAGCGCGTGCGAGGCGTTTGGGGATCCGCAGTTTTGTTGTAGTGAGGCGTACGCTACGCCCGACACGTGTCAACCGTCGGCGTACTCGCTGTTTTTCAAGCATGCTTGCCCACGCTCGTATAGCTACGCGTACGACGACAAGACGAGTACGTACACGTGCGCCTCCGCCGACTACGTCATCATTTTCTGCCCCTTGCCTTATACAAGTCTAAAGGTGTTGGGAGCTCGAAAAGATGGGGCCCCACTACCACTGGTTAACAAGACCATGATGTACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

275

Amino Acids

29.13

Weight (kDa)

8.74

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 31 - 246 4.2e-82 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 4 cut(s) 131, 362, 427, 468
AccB7I CCANNNNNTGG 1 cut(s) 210
AccBSI CCGCTC 2 cut(s) 148, 522
AccI GTMKAC 1 cut(s) 477
AccII CGCG 4 cut(s) 167, 323, 551, 674
AciI CCGC 7 cut(s) 100, 146, 288, 329, 522, 572, 711
AclWI GGATC 3 cut(s) 12, 563, 576
AcoI YGGCCR 1 cut(s) 342
AcvI CACGTG 3 cut(s) 434, 612, 702
AcyI GRCGYC 2 cut(s) 132, 387
AfaI GTAC 9 cut(s) 201, 407, 442, 596, 629, 677, 694, 698, 818
AfiI CCNNNNNNNGG 2 cut(s) 210, 437
AflIII ACRYGT 4 cut(s) 609, 611, 672, 699
AgsI TTSAA 3 cut(s) 82, 484, 643
AhlI ACTAGT 1 cut(s) 85
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 4 cut(s) 74, 393, 669, 770
AluI AGCT 4 cut(s) 74, 393, 669, 770
Alw21I GWGCWC 1 cut(s) 772
AlwI GGATC 3 cut(s) 12, 563, 576
Ama87I CYCGRG 1 cut(s) 292
AoxI GGCC 3 cut(s) 113, 342, 783
ApaI GGGCCC 1 cut(s) 787
Asp700I GAANNNNTTC 1 cut(s) 124
AspLEI GCGC 6 cut(s) 134, 169, 323, 505, 551, 707
AspS9I GGNCC 4 cut(s) 114, 189, 783, 784
AsuC2I CCSGG 4 cut(s) 117, 177, 187, 258
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 292
AvaII GGWCC 1 cut(s) 189
BaeGI GKGCMC 2 cut(s) 497, 787
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 4 cut(s) 131, 362, 427, 468
BanII GRGCYC 3 cut(s) 232, 772, 787
BbrPI CACGTG 3 cut(s) 434, 612, 702
Bbv12I GWGCWC 1 cut(s) 772
BbvCI CCTCAGC 1 cut(s) 506
BccI CCATC 1 cut(s) 773
BceAI ACGGC 3 cut(s) 156, 376, 502
BciT130I CCWGG 1 cut(s) 499
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 4 cut(s) 117, 177, 187, 258
BcuI ACTAGT 1 cut(s) 85
BfaI CTAG 1 cut(s) 86
BfoI RGCGCY 2 cut(s) 135, 506
BfuI GTATCC 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 100, 289
BlsI GCNGC 2 cut(s) 101, 290
Bme1390I CCNGG 5 cut(s) 117, 177, 187, 258, 499
Bme18I GGWCC 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 4 cut(s) 114, 189, 783, 784
BmrFI CCNGG 5 cut(s) 117, 177, 187, 258, 499
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 2 cut(s) 408, 443
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 612
BplI GAGNNNNNCTC 2 cut(s) 53, 85
Bpu10I CCTNAGC 1 cut(s) 506
BpuMI CCSGG 4 cut(s) 117, 177, 187, 258
BsaAI YACGTR 4 cut(s) 434, 612, 696, 702
BsaHI GRCGYC 2 cut(s) 132, 387
BsaJI CCNNGG 2 cut(s) 497, 498
BsaWI WCCGGW 1 cut(s) 245
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 437
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 3 cut(s) 163, 469, 801
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 2 cut(s) 497, 498
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 2 cut(s) 210, 437
BseMII CTCAG 3 cut(s) 72, 84, 497
BseNI ACTGG 3 cut(s) 163, 469, 801
BseSI GKGCMC 2 cut(s) 497, 787
Bsh1236I CGCG 4 cut(s) 167, 323, 551, 674
BshFI GGCC 3 cut(s) 115, 344, 785
BshNI GGYRCC 4 cut(s) 131, 362, 427, 468
BsiHKAI GWGCWC 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 8 cut(s) 117, 129, 177, 187, 246, 258, 312, 332
BsiWI CGTACG 2 cut(s) 594, 675
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 2 cut(s) 210, 437
BsmFI GGGAC 1 cut(s) 169
BsnI GGCC 3 cut(s) 115, 344, 785
BsoBI CYCGRG 1 cut(s) 292
Bsp120I GGGCCC 1 cut(s) 783
Bsp1286I GDGCHC 4 cut(s) 232, 497, 772, 787
Bsp143I GATC 2 cut(s) 4, 568
BspACI CCGC 7 cut(s) 100, 146, 288, 329, 522, 572, 711
BspANI GGCC 3 cut(s) 115, 344, 785
BspCNI CTCAG 3 cut(s) 71, 83, 498
BspFNI CGCG 4 cut(s) 167, 323, 551, 674
BspPI GGATC 3 cut(s) 12, 563, 576
BspT107I GGYRCC 4 cut(s) 131, 362, 427, 468
BsrBI CCGCTC 2 cut(s) 148, 522
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 3 cut(s) 163, 469, 801
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 497, 498
BssMI GATC 2 cut(s) 4, 568
BssNI GRCGYC 2 cut(s) 132, 387
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 1 cut(s) 621
BstACI GRCGYC 2 cut(s) 132, 387
BstBAI YACGTR 4 cut(s) 434, 612, 696, 702
BstC8I GCNNGC 4 cut(s) 333, 553, 648, 652
BstDEI CTNAG 3 cut(s) 58, 70, 506
BstF5I GGATG 1 cut(s) 458
BstFNI CGCG 4 cut(s) 167, 323, 551, 674
BstH2I RGCGCY 2 cut(s) 135, 506
BstHHI GCGC 6 cut(s) 134, 169, 323, 505, 551, 707
BstKTI GATC 2 cut(s) 7, 571
BstMBI GATC 2 cut(s) 4, 568
BstMWI GCNNNNNNNGC 7 cut(s) 41, 140, 341, 427, 511, 557, 666
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 1 cut(s) 650
BstPAI GACNNNNGTC 1 cut(s) 612
BstSCI CCNGG 5 cut(s) 115, 175, 185, 256, 497
BstSLI GKGCMC 2 cut(s) 497, 787
BstSNI TACGTA 1 cut(s) 696
BstUI CGCG 4 cut(s) 167, 323, 551, 674
BstX2I RGATCY 1 cut(s) 568
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 568
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 3 cut(s) 115, 344, 785
BtsCI GGATG 1 cut(s) 458
BtsIMutI CAGTG 2 cut(s) 156, 794
Cac8I GCNNGC 4 cut(s) 333, 553, 648, 652
CfoI GCGC 6 cut(s) 134, 169, 323, 505, 551, 707
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 114, 189, 783, 784
CseI GACGC 1 cut(s) 262
Csp6I GTAC 9 cut(s) 200, 406, 441, 595, 628, 676, 693, 697, 817
CviAII CATG 2 cut(s) 647, 811
CviQI GTAC 9 cut(s) 200, 406, 441, 595, 628, 676, 693, 697, 817
DdeI CTNAG 3 cut(s) 58, 70, 506
DinI GGCGCC 1 cut(s) 133
DpnI GATC 2 cut(s) 6, 570
DpnII GATC 2 cut(s) 4, 568
EaeI YGGCCR 1 cut(s) 342
EciI GGCGGA 1 cut(s) 700
Ecl136II GAGCTC 1 cut(s) 770
Eco105I TACGTA 1 cut(s) 696
Eco24I GRGCYC 3 cut(s) 232, 772, 787
Eco47I GGWCC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 770
Eco72I CACGTG 3 cut(s) 434, 612, 702
Eco88I CYCGRG 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 783, 784
EcoRII CCWGG 1 cut(s) 497
EcoT38I GRGCYC 3 cut(s) 232, 772, 787
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
FaeI CATG 2 cut(s) 650, 814
FaiI YATR 7 cut(s) 112, 241, 384, 648, 666, 747, 812
FaqI GGGAC 1 cut(s) 169
FatI CATG 2 cut(s) 646, 810
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 2 cut(s) 100, 289
FokI GGATG 1 cut(s) 465
FriOI GRGCYC 3 cut(s) 232, 772, 787
Fsp4HI GCNGC 2 cut(s) 100, 289
FspBI CTAG 1 cut(s) 86
GlaI GCGC 6 cut(s) 133, 168, 322, 504, 550, 706
GluI GCNGC 2 cut(s) 100, 289
HaeII RGCGCY 2 cut(s) 135, 506
HaeIII GGCC 3 cut(s) 115, 344, 785
HapII CCGG 8 cut(s) 117, 129, 177, 187, 246, 258, 312, 332
HgaI GACGC 1 cut(s) 262
HhaI GCGC 6 cut(s) 134, 169, 323, 505, 551, 707
Hin1I GRCGYC 2 cut(s) 132, 387
Hin1II CATG 2 cut(s) 650, 814
Hin6I GCGC 6 cut(s) 132, 167, 321, 503, 549, 705
HinP1I GCGC 6 cut(s) 132, 167, 321, 503, 549, 705
HincII GTYRAC 4 cut(s) 91, 478, 617, 802
HindII GTYRAC 4 cut(s) 91, 478, 617, 802
HinfI GANTC 1 cut(s) 235
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 8 cut(s) 117, 129, 177, 187, 246, 258, 312, 332
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 8 cut(s) 67, 91, 351, 400, 478, 617, 699, 802
Hpy188I TCNGA 3 cut(s) 61, 234, 462
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 8 cut(s) 67, 91, 351, 400, 478, 617, 699, 802
Hpy99I CGWCG 4 cut(s) 44, 479, 625, 683
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 7 cut(s) 338, 387, 433, 611, 695, 701, 720
HpyCH4V TGCA 2 cut(s) 243, 456
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 140, 341, 427, 511, 557, 666
HpyF3I CTNAG 3 cut(s) 58, 70, 506
HpySE526I ACGT 7 cut(s) 338, 387, 433, 611, 695, 701, 720
Hsp92I GRCGYC 2 cut(s) 132, 387
Hsp92II CATG 2 cut(s) 650, 814
HspAI GCGC 6 cut(s) 132, 167, 321, 503, 549, 705
KasI GGCGCC 1 cut(s) 131
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 2 cut(s) 4, 568
LmnI GCTCC 4 cut(s) 148, 531, 546, 767
LweI GCATC 2 cut(s) 408, 443
MaeI CTAG 1 cut(s) 86
MaeII ACGT 7 cut(s) 338, 387, 433, 611, 695, 701, 720
MaeIII GTNAC 2 cut(s) 260, 274
MalI GATC 2 cut(s) 6, 570
MbiI CCGCTC 2 cut(s) 148, 522
MboI GATC 2 cut(s) 4, 568
MboII GAAGA 1 cut(s) 46
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 4 cut(s) 232, 497, 772, 787
MluI ACGCGT 1 cut(s) 672
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 485
MroNI GCCGGC 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 801
MspI CCGG 8 cut(s) 117, 129, 177, 187, 246, 258, 312, 332
MspR9I CCNGG 5 cut(s) 117, 177, 187, 258, 499
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 4 cut(s) 167, 323, 551, 674
MwoI GCNNNNNNNGC 7 cut(s) 41, 140, 341, 427, 511, 557, 666
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 4 cut(s) 117, 177, 187, 258
NdeII GATC 2 cut(s) 4, 568
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 2 cut(s) 650, 814
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PasI CCCWGGG 1 cut(s) 498
PcsI WCGNNNNNNNCGW 4 cut(s) 603, 608, 617, 678
PdiI GCCGGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 124
Pfl23II CGTACG 2 cut(s) 594, 675
PflFI GACNNNGTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 185
PkrI GCNGC 2 cut(s) 101, 290
PleI GAGTC 1 cut(s) 229
PluTI GGCGCC 1 cut(s) 135
PmaCI CACGTG 3 cut(s) 434, 612, 702
PmlI CACGTG 3 cut(s) 434, 612, 702
PpsI GAGTC 1 cut(s) 229
Ppu21I YACGTR 4 cut(s) 434, 612, 696, 702
PshAI GACNNNNGTC 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 772
Psp6I CCWGG 1 cut(s) 497
PspCI CACGTG 3 cut(s) 434, 612, 702
PspGI CCWGG 1 cut(s) 497
PspLI CGTACG 2 cut(s) 594, 675
PspOMI GGGCCC 1 cut(s) 783
PspPI GGNCC 4 cut(s) 114, 189, 783, 784
PsuI RGATCY 1 cut(s) 568
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 9 cut(s) 201, 407, 442, 596, 629, 677, 694, 698, 818
RsaNI GTAC 9 cut(s) 200, 406, 441, 595, 628, 676, 693, 697, 817
SacI GAGCTC 1 cut(s) 772
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 2 cut(s) 100, 289
Sau3AI GATC 2 cut(s) 4, 568
Sau96I GGNCC 4 cut(s) 114, 189, 783, 784
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 5 cut(s) 117, 177, 187, 258, 499
SduI GDGCHC 4 cut(s) 232, 497, 772, 787
SfaNI GCATC 2 cut(s) 408, 443
SfoI GGCGCC 1 cut(s) 133
SinI GGWCC 1 cut(s) 189
SnaBI TACGTA 1 cut(s) 696
SpeI ACTAGT 1 cut(s) 85
SphI GCATGC 1 cut(s) 650
SsiI CCGC 7 cut(s) 100, 146, 288, 329, 522, 572, 711
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 772
StyD4I CCNGG 5 cut(s) 115, 175, 185, 256, 497
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 7 cut(s) 341, 390, 436, 614, 698, 704, 723
TaqI TCGA 3 cut(s) 303, 477, 772
TauI GCSGC 2 cut(s) 102, 291
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 2 cut(s) 163, 801
TseFI GTSAC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 274
TspGWI ACGGA 2 cut(s) 187, 384
TspRI CASTG 2 cut(s) 163, 801
Tth111I GACNNNGTC 1 cut(s) 719
Van91I CCANNNNNTGG 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 189
XceI RCATGY 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 1 cut(s) 86
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.